Brassica Ontology
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Curators Sophie Durand, INRAe
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Lead Center
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=Term, 
=Trait, 
=Method, and 
=Scale
Variables
Concept details
| Key | Value |
|---|---|
| method_id | CO_348:3100011 |
| method_name | HPLC (High performance liquid chromatography) analysis - Measurement |
| ontology_id | CO_348 |
| ontology_name | Brassica |
| method_class | Measurement |
| method_description | A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents |
| language | EN |