Rice Ontology 
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Concept details
| Key | Value |
|---|---|
| method_id | CO_320:0000326 |
| method_name | Pollen sterility calculation |
| ontology_id | CO_320 |
| ontology_name | Rice |
| method_class | Computation |
| method_description | Collect pollen from at least ten florets at anthesis, fix in 70% alcohol. Place 2-3 anthers from each of five florets on a glass slide, and squeeze the pollen out in a drop of 1% iodine potassium iodide (I2-KI) solution with a spear-shaped needle. Fertile pollen grains will be round and well stained, sterile pollen grains will be unstained or partially stained, and withered or round. Using a microscope at magnification 10x10, count fertile and sterile grains in at least three microscopic fields, calculate the proportion that are sterile |
| method_reference | IRIS:196, SES:21A |
| language | EN |
| created_at | 2023-07-25-13:52:48 |