StmLy StmLy_E_1to9 KAUST Clara Stanschewski SdHlW_M_g SdHlW KAUST Clara Stanschewski KAUST Clara Stanschewski PltNoHv PltNoHv_Ct_plant PcleCol PcleCol_E_4to16 KAUST Clara Stanschewski HI KAUST Clara Stanschewski HI_C_pct Clara Stanschewski SdPrm_Comp_mm KAUST SdPrm Quinoa ontology PcleDen_E_1to7 PcleDen KAUST Clara Stanschewski PcleLfs_E_1to7 KAUST Clara Stanschewski PcleLfs SdWRepPt SdWRepPt_M_g KAUST Clara Stanschewski Clara Stanschewski SdAvgRed SdAvgRed_Comp_rgb KAUST SdAvgBlue_Comp_rgb SdAvgBlue KAUST Clara Stanschewski KAUST PcleShp Clara Stanschewski PcleShp_E_1to3 KAUST Clara Stanschewski LfMgn LfMgn_E_1to5 SdGerm SdGerm_M_pct Clara Stanschewski KAUST BGDBM KAUST Clara Stanschewski BGDBM_M_g SdWpPt_C_g SdWpPt KAUST Clara Stanschewski StmDiaBse StmDiaBse_M_mm KAUST Clara Stanschewski SdSht_E_1to7 KAUST Clara Stanschewski SdSht StmLodg_E_1to9 KAUST Clara Stanschewski StmLodg KAUST Clara Stanschewski PtHt_M_cm PtHt Brnch KAUST Clara Stanschewski Brnch_E_1to7 Clara Stanschewski SigPcle KAUST SigPcle_Ct_panicle acronym AGDBM_M_g AGDBM KAUST Clara Stanschewski PcleLng PcleLng_M_cm KAUST Clara Stanschewski KAUST Clara Stanschewski PlotHomo_E_1to7 PlotHomo KAUST Clara Stanschewski TGW_M_g TGW KAUST Clara Stanschewski AxlPig_E_0to1 AxlPig Clara Stanschewski SdA SdA_Comp_mm KAUST PlotCov PlotCov_E_1to9 KAUST Clara Stanschewski StmAng KAUST Clara Stanschewski StmAng_E_1to7 Clara Stanschewski KAUST StmBrk StmBrk_E_1to9 KAUST Clara Stanschewski GH GH_E_1to7 KAUST Clara Stanschewski PcleAng PcleAng_E_1to7 SdAvgGreen KAUST Clara Stanschewski SdAvgGreen_Comp_rgb Clara Stanschewski PltNoHvRp KAUST PltNoHvRp_Ct_plant SdWpPlot SdWpPlot_M_g KAUST Clara Stanschewski KAUST Clara Stanschewski StmStr StmStr_E_0to1 attribute KAUST Clara Stanschewski StmLfShp StmLfShp_E_1to2 StmCol_E_4to15 StmCol KAUST Clara Stanschewski GrStg Clara Stanschewski KAUST GrStg_E_BBCH KAUST Clara Stanschewski StmDiaPcle_M_mm StmDiaPcle entity Panicle density 1-3-5-7 Harvest index computation None Panicle leafiness 1-3-5-7 None Seed average red color computation AGDBM Cutting plants at the very base with secateurs and drying the entire above-ground section of the plant. Recording total dry weight and number of plants that make up total dry weight. Divide total weight with number of plants to give weight per plant Above-ground dry biomass Plant Plant above-ground dry biomass Stem Lodging incidence StmLodg Stem lodging incidence Plants are prostrate, on or near the ground, with intact stems; assessing the percentage of the plot affected SdAvgBlue Seed average blue color Numerical seed color blue component, RGB B Component from image analysis average blue color Seed Seed yield for representative plants measurement None Plot Percentage of the plot covered, 1 = poor to 7 = good establishment Coverage PlotCov Plot coverage None Stem lying incidence estimation Stem diameter near plant base measurement None SdAvgGreen Seed average green color Numerical seed color green value, RGB G Component from image analysis average green color Seed Panicle color 13-16-4-7-15 Thickness of the stem measured just underneath the main panicle Stem diameter under the panicle Stem Diameter under the panicle StmDiaPcle Plant branchiness 1-3-5-7 Number of representative plants harvested counting None panicle Panicle axis angle estimation None None Number of significant panicles counting Panicle axis angle 1-3-5-7 None Plant above-ground dry biomass measurement Categorized into green (13), red (4), or no pigmentation (15) StmCol Color Stem color Stem Stem color estimation None Panicle Panicle axis angle Axis angle The angle at which the majority of panicle axes are leaning, measured between an upright panicle on the verticle and a panicle pointing towards the ground PcleAng Stem striae Striae Presence (1) absence (0) scoring of stem streaks or stripes Stem StmStr Density Scored from 1 = lax (loose) with panicle axes easily visible to 7 = tight and compact panicles PcleDen Panicle Panicle density None Plant below-ground dry biomass measurement Seed shattering estimation None Panicle shape Shape Classified into one of the three categories: glomerulate, intermediate, or amarantiform Panicle PcleShp Seed average red color Seed average red color SdAvgRed Numerical seed color red value, RGB R Component from image analysis StmDiaBse Diameter at the plant base Stem Thickness of the stem measured with calipers at the middle of the bottom third of the plant stem Stem diameter near plant base Panicle color Color Panicle Categorized according to the color scoring card PcleCol Axil pigmentation estimation None Plot coverage 1-3-5-7-9 Seed average blue color computation None Scored from 1 = no leaves to 7 = many leaves Panicle Panicle leafiness Leafiness PcleLfs None Leaf margin estimation Seed yield per plant computation None SdWpPlot Seed Seed yield per plot Yield total Harvesting the panicles remaining per plot while excluding borders, and adding the weight to that from the four representative plants, seed dried in oven to constant weight Panicle color estimation None None Stem striae estimation g Below-ground dry biomass If possible, root biomass could also be measured (especially when plants are growing in sandy soil) BGDBM Plant Plant below-ground dry biomass Seed Seed yield for representative plants SdWRepPt Seed mass of approximately four representative plants that were harvested from the center of the plot (seed should be dried to constant weight) Yield of representative plants Plant branchiness estimation None LfMgn Margin Leaf margin Leaf margins are classified into 3 groups Leaf Stem striae 0-1 Growth stage Phenological growth stage; very important to record at mature phenotyping Plant Growth stage GrStg Score for the overall amount of side branches of representative plants in a plot, ignoring very small and spindly branches, ranging from 1 = no branches to 7 = bushy plant with many major lateral branches Plant branchiness Branchiness Brnch Plant None Plant height measurement Length Length of the primary panicle measured with the same stick. Measured from the base of the panicle to the tip PcleLng Panicle length Panicle Stem lodging incidence estimation None Count of the number of significant panicles, i.e., larger panicles, near the top of the plant, harvestable, that provide a major contribution to the seed harvested from the plant Number of significant panicles SigPcle Number of significant Panicle variable_of BBCH plant Stem color 13-4-15 Stem lying incidence 1-3-5-7-9 Plot Total number of plants that were harvested of the plot. PltNoHv Plants harvested Number of plants harvested per plot Stem lodging incidence 1-3-5-7-9 rgb None Panicle shape estimation Angle The angle at which the majority of plants are leaning, measured between the vertical axis and the horizontal axis StmAng Stem Stem angle StmBrk Breakage incidence Stem Stems are broken or detached, assessing the percentage of the plot affected Stem breakage incidence Harvest index Yield/ above-ground biomass HI Havest index Plant Plant growth habit 1-3-5-7 None Panicle length measurement Leaf margin 1-3-5 Seed Seed yield per plant Total harvested seed mass per plant may be calculated from number of plants harvested and the seed mass of all plants in the plot (SdWtpPlot/PltNoHv) SdWpPt Yield per plant mm TGW Thousand Grain Weight (TGW), the weight of 1000 seeds Seed Thousand grain weight Thousand Grain Weight Plot coverage estimation None None Stem leaf shape estimation Panicle shape 1-3-5 Stem leaf shape Leaves of the stem are categorized into two groups: rhomboidal (1) and triangular (2) Stem StmLfShp Leaf shape Lying incidence Stem lying incidence StmLy Stem of the plant is not emerging straight up from the soil but has a kink at the base, growing along the ground before rising; assessing the percentage of the plot affected Stem Stem diameter under the panicle measurement None Seed yield per plot measurement None Thousand Grain Weight measurement None Seed Hectoliter weight, or specific weight of seeds: Estimation of density, determined by weighing all seeds fitting into a 100 ml volume Seed hectoliter weight Hectoliter weight SdHlW Axil pigmentation 0-1 None Stem breakage incidence estimation None Stem angle estimation Seed shattering 1-3-5-7 Number of representative plants harvested PltNoHvRp Count the number of plants that were harvested seperately from the majority of the plot, as representative plants. Plot Representative plants harvested Seed SdPrm Average perimeter of the seeds from image analysis average perimeter Seed average perimeter None Seed average area computation % Stem angle 1-3-5-7 None Panicle density estimation Germination rate SdGerm Seed Tested in petri dishes (%) Seed germination rate Plot population homogeneity 1-3-5-7 None Seed average perimeter computation cm SdA Seed average area Average area of the seeds from image analysis average area Seed SdSht Seed shattering Shattering Grain persistence in the plant at physiological maturity. Assessing how easy seeds fall off the panicle upon light touch: 1 = No seeds falling to 7 = a lot of seeds falling Seed Axil AxlPig Presence (1) absence (0) scoring of pigmented axils Pigmentation Axil pigmentation scale_of Height of the most representative plants of the plot, usually from the middle of the plot, measured with a long measuring stick from soil to the tip of the panicle. If more than one distinct phenotype is present, more than one plant may be recorded in a new row of the spreadsheet, with all phenotypes that are differing recorded separately PtHt Plant height Plant Height Plot population homogeneity estimation None Seed average green color computation None method_of Plant growth habit estimation None Stem breakage incidence 1-3-5-7-9 GH Four categories of growth habit described in images on the scoring card. Here the focus lies on whether branching is present from the base of the plant and if a main inflorescence can be identified Growth habit Plant growth habit Plant None Growth stage estimation Panicle leafiness estimation None Plot Population homogeneity PlotHomo Judgment of homogeneity of the accession, 1 = homogeneous to 7 = mixed Plot population homogeneity Stem leaf shape 1-2 Number of plants harvested per plot counting None Seed hectoliter weight measurement None None Seed germination rate measurement