pseudostemFreshMass_Meas_g Sebastien Carpentier KU Leuven pseudostem_fresh massFreshPseudostem Sebastien Carpentier KU Leuven numLeaf numLeaves_Count_unitless totalNumLeaves Rachel Chase PsdHght_E Bioversity International Bioversity International Rhiannon Crichton DateColl_Date_dmy Rhiannon Crichton FstExtDiSmptFW_Date_dmy Bioversity International Bioversity International YlLfPercFW_Comp_pct Rhiannon Crichton Tendo Ssali IITA temp44 Bioversity International/IITA Inge Van den Bergh/Danny Coyne temp49 5 other Allan Brown/Guillaume Bauchet (temp.) FngUnSmshdPlpTxt_Est_1to5 IITA-BTI rateWaterLossPerDay waterLossDay_Comp_ml Sebastien Carpentier waterLossDaily KU Leuven Rhiannon Crichton MltHandWt_M_kgPlnt Bioversity International PA_EstResp_Txt Bioversity Noel Madalla Bioversity International Rachel Chase PdcFsn_Est_1to2 no internal symptoms 1 Bioversity PA_EstFemale_Txt Noel Madalla Sebastien Carpentier lengthSum KU Leuven totLeafLength_Meas_cm CmpdTplLbCl_Est_1to5 Bioversity International Rachel Chase strongly bottle-necked (wider under tip than number 2) 4 Rhiannon Crichton PSuckN_Ct_PSuck Bioversity International Bioversity International MlBdShp_Comp_1to3 Rachel Chase Rachel Chase Bioversity International FrtApxPt_Est_1to5 BoiPlantnMois_Meas_0to10 Boiled plantain Moistness scale 0-10 Hermann Antonin KOUASSI CIRAD / UNA, Côte d’Ivoire BlkLfStrkSvrt_Est_Faure IITA-Bioversity International Hermann Antonin KOUASSI BoiPlantnStic_Meas_0to10 CIRAD / UNA, Côte d’Ivoire Boiled plantain Stickiness scale 0-10 Bioversity International Rhiannon Crichton PltDead_Ct_Plnt IITA Tendo Ssali WvlAdlt_Ct_weevil DamEst_Date_dmy Bioversity International Rhiannon Crichton Danny Coyne NmRpFctr_Ct_Nema IITA red-purple 2 Rhiannon Crichton Bioversity International PtlClpFW_Comp_pct Bioversity International Rachel Chase BrctClExtFc_Int_1to8 Bioversity International Rachel Chase PMargClasp_Est_1or2 heightPseudostem lengthPseudostem_Meas_cm heightPS Sebastien Carpentier KU Leuven 5 > 100 egg masses Sebastien Carpentier KU Leuven areaRatio leafAreaRatio_Comp_unitless areaPlant_per_areaTot MlBdLngthRt_M_1to3 Rachel Chase Bioversity International Tendo Ssali IITA temp42 yellow 3 Rhiannon Crichton FlwHvstTime_Comp_d Bioversity International Bioversity International/IITA temp52 Inge Van den Bergh/Danny Coyne red-purple 4 Bioversity International BlkLfStrkSvrtLfNb_Ct_grd5 Rhiannon Crichton IITA-Bioversity International NSpotLfProp_Comp_idx Guillaume Bauchet BnchMatStg_Est_1to2 IITA-BTI transpSyst waterLossSyst_Meas_ml waterLossSystem Sebastien Carpentier KU Leuven 2 intermeditae Bioversity International HandAvgWt_M_kgPlnt Rhiannon Crichton Inge Van den Bergh Bioversity International PStmDiscol_Est_cm IITA WvlAdltBdWght_M_mg Tendo Ssali IITA-BTI Guillaume Bauchet FrtDiam_M_cm Rhiannon Crichton Bioversity International SuckHtShtStg_M_cm Tendo Ssali IITA DmgPrphl_M_% Bioversity International Rachel Chase FrtApxFlwRlt_Est_1to3 GdBnch_Est_1to2 Allan Brown IITA Noel Madalla Bioversity Selection criteria for genotype that was ranked highest by female respondents during preference scoring exercise Bioversity International BnchWt_M_kg Rhiannon Crichton curved (sharp curve) 4 5 firm orange red 10 Bioversity International temp16 Rhiannon Crichton 1 cream Rachel Chase Bioversity International BlotchA_Est_1to4 Rhiannon Crichton HndFrtAvgNum_Ct_Frt Bioversity International PA_EstMale_Txt Noel Madalla Bioversity green 2 Bioversity International Rhiannon Crichton PltDamDesc_Est_txt PMargCol_Esr_1to16 Bioversity International Rachel Chase DmgIdxInn_M_% IITA Tendo Ssali 3 < 1/3 discoloured Rhiannon Crichton Shoot_Date_dmy Bioversity International AvgWvlLvHdCps_M_µM Tendo Ssali IITA PratyCofNumRtWt2_Comp_Nema Danny Coyne IITA MeloiNumRtWt2_Comp_Nema Danny Coyne IITA Rhiannon Crichton PtYd_Comp_TnHaY Bioversity International Bioversity International Rhiannon Crichton PltHvstd_Ct_Plnt IITA-BTI Guillaume Bauchet FrtGrth_M_cm FrtftPdclLgth_M_mm Bioversity International Rachel Chase IITA Tendo Ssali AvgWvlAdltBdLgt_M_µM PltFstLfSptd_Ct_leaf Rhiannon Crichton Bioversity International PetColpPres_Est_0or1 Bioversity International Inge Van den Bergh 2 13-16 pointed 1 Rachel Chase Bioversity International MgnBhvPtCnlThrdLf_Est_1to5 PltEst_Date_ymd IITA-BTI Guillaume Bauchet dislike 2 Danny Coyne IITA RadoNumRtWt2_Comp_Nema Amos Alakonya-Rhiannon Crichton IITA-Bioversity International FstYngLfsptd_Est_dmy MlRchsApp_Estm_1to7 Rachel Chase Bioversity International PMargColL_Est_1or2 Rachel Chase Bioversity International Bioversity International Inge Van den Bergh PetColpPres_Est_1or2 growthPS_per_leafFormed Sebastien Carpentier pseudostemHeightIncreasePerFormedLeaf_Comp_cm KU Leuven Selection criteria for genotype that was ranked lowest by female respondents during preference scoring exercise Noel Madalla Bioversity root_fresh massFreshRoot Sebastien Carpentier KU Leuven rootFreshMass_Meas_g Sebastien Carpentier KU Leuven lengthSingleLeaf singleLeafLength_Meas_cm lengthPerLeaf NARO, Uganda MatSurfYellV_Meas_0to10 Matooke surface yellowness measurement scale 0-10 Elizabeth Kakhasa Elizabeth Kakhasa MatSweet_Meas_0to10 Matooke sweetness measurement scale 0-10 NARO, Uganda BlkLfStrkSvrtLfNb_Ct_grd0 Rhiannon Crichton Bioversity International Hermann Antonin KOUASSI Boiled plantain Chewiness by number of chews CIRAD / UNA, Côte d’Ivoire BoiPlantnChew_Meas_number of chews 4 First-spot stage: the streaks broaden and become more or less fusiform or elliptical in outline and a water soaked border appears around each lesion. orange red 10 Bioversity International/IITA Inge Van den Bergh/Danny Coyne DeadRtNum2_Ct_Rt Bioversity International Rhiannon Crichton SuckNum_Ct_Suck FrtCvShp_Est_1to5 Rachel Chase Bioversity International Bioversity International PStmSplitPres_Est_1or2 Inge Van den Bergh totLeafArea_Meas_cm2 areaTotLeaves Sebastien Carpentier KU Leuven areaLeaves BrctImbrcApxMlBd_Est_1to3 Rachel Chase Bioversity International 7 Other PratyGooNumRtWt2_Comp_Nema Danny Coyne IITA Danny Coyne IITA NmNumSlWt Inge Van den Bergh LfChgPres_Est_1or2 Bioversity International Bioversity International/IITA Inge Van den Bergh/Danny Coyne temp45 Selection criteria for genotype that was ranked second lowest by female respondents during preference scoring exercise Noel Madalla Bioversity large blotches 3 AvgWvlAdltBdWght_M_mg Tendo Ssali IITA WvlAdltBdLgt_M_µM Tendo Ssali IITA PStmSplitPres_Est_0or1 Bioversity International Inge Van den Bergh Bioversity International Rachel Chase BlotchA_Est_1to5 BoiPlantnSwt_Meas_0to10 Boiled plantain Sweetness scale 0-10 Hermann Antonin KOUASSI CIRAD / UNA, Côte d’Ivoire Bioversity International Rachel Chase FrtApxFlwRlt_Est_1to4 no visible sign of fusion 1 10 bright yellow NARO, Uganda Matooke astringency measurement scale 0-10 MatAstr_Meas_0to10 Elizabeth Kakhasa Rhiannon Crichton AvgPulpDiam_M_mm Bioversity International FngSmshdPlpSmth_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) IITA-BTI isolated points of discolouration in vascular tissue 2 Altus Viljoen temp38 Stellenbosch University FstExtDiSmptFW_Date_ymd Rhiannon Crichton Bioversity International Sebastien Carpentier plantGrowth_dry KU Leuven growthDry growthDry_Comp_g Noel Madalla Bioversity Selection criteria for genotype that was ranked third highest by female respondents during preference scoring exercise Bioversity International Rachel Chase FstBrctApxShp_Est_1to4 2 yellow KU Leuven rwcLeaf rwcLeaf_Comp_unitless leaf_rwc Sebastien Carpentier PltDth_Comp_d Rhiannon Crichton Bioversity International 9 other Sebastien Carpentier KU Leuven rwcPseudostem_Comp_unitless ps_rwc rwcPseudostem curved (sharp curve) 3 Bioversity International FrtWtAvg_M_g Rhiannon Crichton IITA-BTI FrtIntLng_M_cm Guillaume Bauchet 3 asymmetrical 26-30 cm 4 BoiPlantnFirm_Meas_0to10 Boiled plantain Firmness scale 0-10 Hermann Antonin KOUASSI CIRAD / UNA, Côte d’Ivoire 1 brown PratyNumRtWt2_Comp_Nema Danny Coyne IITA WvlAdltHdCps_M_µM Tendo Ssali IITA IITA-BTI Guillaume Bauchet AvgFrtIntLng_M_cm Bioversity International PlntgDisTim_Comp_d Inge Van den Bergh Rhiannon Crichton PStmHt_M2_cm Bioversity International 8 other 3 fat (w/y equal to or more than 0.55) IITA Tendo Ssali AvgWvlLvBdLgt_M_µM HndNumMsrd_Ct_Hnd Rhiannon Crichton Bioversity International OffTypEst_Date_ymd IITA-BTI Guillaume Bauchet CigLfUnrBrnStgB_Date_ymd Bioversity International Rhiannon Crichton few flower relicts (< 20% of the fruits with relicts) 2 FngSmshdPlpTxt_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) IITA-BTI Rhiannon Crichton Bioversity International CauDam_Est_txt IITA-BTI Guillaume Bauchet DateColl_Date_ymd Sebastien Carpentier waterLossPlant KU Leuven waterLossPlant_Meas_ml transpPlant 3 other (specify on answer sheet) Bioversity International FrtExtLng_M_cm Rhiannon Crichton BnchPst_Estm_1to5 Bioversity International Rachel Chase Rhiannon Crichton Bioversity International MltFrtWt_M_gFruit Bioversity International temp8 Rhiannon Crichton temp2 Rhiannon Crichton Bioversity International 1 open with margins spreading pointed 1 Tendo Ssali TrpNum_Ct_weeviltrap IITA less than or equal to 20 cm 1 Amos Alakonya-Rhiannon Crichton StdLf_Ct_leaf IITA-Bioversity International Rachel Chase BrctClExtFc_Est_1to16 Bioversity International MlBdShd_Comp_1to3 Bioversity International Rachel Chase temp22 Bioversity International Rhiannon Crichton Rachel Chase PMargCol_Esr_1to4 Bioversity International Elizabeth Kakhasa Matooke surface homogeneity of colour measurement scale 1-10 NARO, Uganda MatSurfHomColV_Meas_0to10 Rachel Chase Bioversity International BrctBhvBfFll_Est_1to2 MatMoistM_Meas_0to10 Elizabeth Kakhasa Matooke moisture in mouth measurement scale 0-10 NARO, Uganda Bioversity Noel Madalla Selection criteria for genotype that was ranked lowest by male respondents during preference scoring exercise Bioversity International CigLfPigm_Est_1to3 Rachel Chase PulpDiam_M_cm IITA-BTI Guillaume Bauchet absent 0 soft 0 IITA WvlLv_Ct_weevil Tendo Ssali pseudostemDryMass_Meas_g massDryPseudostem Sebastien Carpentier KU Leuven ps_dry Bioversity International AvgFrtDiam_M_mm Rhiannon Crichton FngSmshdPlpArm_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) IITA-BTI moldable 10 Bioversity International PltSpcg_Comp_m2 Rhiannon Crichton purple 5 Bioversity International Rhiannon Crichton DthPrp_Comp_DdPltRt RotyNumSlWt_CompNema Danny Coyne IITA HelicoNumSlWt_Comp_Nema Danny Coyne IITA Rhiannon Crichton Bioversity International SptPsdStmPercFW_Comp_pct 2 lengthily pointed (like plantain) Rhiannon Crichton temp12 Bioversity International Rhiannon Crichton Bioversity International temp21 Bioversity International PeelThck_M_mm Rhiannon Crichton Tendo Ssali WvlLvBdWght_M_mg IITA 4 extensive pigmentation Bioversity International CmpdTplLbCl_Est_1to16 Rachel Chase 1 Initial speck stage: faint, minute, reddish-brown specks on the lower surface of the leaf. 2 medium (20 cm < y < 30 cm) winged and not clasping the pseudostem 2 Bioversity International HvstDth_Comp_d Rhiannon Crichton 3 low shouldered (x/y more than or equal to 0.30) Rhiannon Crichton temp40 Bioversity International 1-2 egg masses 1 Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd6 Bioversity International other 10 Bioversity International/IITA Inge Van den Bergh/Danny Coyne temp53 DET_Comp_d Rhiannon Crichton Bioversity International Bioversity International RkPvsMktLf_Comp_leafrank Rhiannon Crichton black (anthers aborted) 7 Bioversity International Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd3 IITA DmgIdxOut_M_% Tendo Ssali Elizabeth Kakhasa NARO, Uganda Matooke stickiness in hand measurement scale 0-10 MatStickT_Meas_0to10 1 less than or equal to 15 cm BlotchCol_Est_1to4 Rachel Chase Bioversity International IITA WvlLvHdCps_M_µM Tendo Ssali Bioversity International Rachel Chase CmpdTplMnCl_Est_1to6 Allan Brown/Guillaume Bauchet (temp.) IITA-BTI FngUnSmshdPlpSmth_Est_1to5 MlBdLngth_M_1to3 Rachel Chase Bioversity International 3 watery green Rhiannon Crichton temp31 Bioversity International green 7 16-20 cm 2 5 margins overlapping low intensity 0 2 fusarium wilt temp37 Stellenbosch University Altus Viljoen Rhiannon Crichton BlkLfStrkLfFrStg1_Date_dmy Bioversity International PS_CompResp_PrefScale Noel Madalla Bioversity Rhiannon Crichton Bioversity International FstYngLfsptd_Est_ymd red 8 TEtotalDry transpirationEfficiency_Comp_gml Sebastien Carpentier TE_dry KU Leuven KU Leuven leafFreshMass_Meas_g massFreshLeaf leaf_fresh Sebastien Carpentier IITA WvlAdltMsrd_Ct_weevil Tendo Ssali temp47 Bioversity International/IITA Inge Van den Bergh/Danny Coyne 1 revolute (rolling) yellow 1 temp48 Bioversity International/IITA Inge Van den Bergh/Danny Coyne First-spot stage: the streak broadens and becomes more or less fusiform and elliptical in outline and similar to the first-spot stage of Sigatoka (Leach 1946). The transition from streak to spot is further characterised by the development of a light brown, water-soaked border around the spot. This water-soaked effect is especially clear in the early morning, when dew is still present on the leaf, or after rain. 4 Bioversity International FrtGrth_M_mm Rhiannon Crichton DateDeath_Est_ymd IITA-BTI Guillaume Bauchet Rachel Chase Bioversity International BlotchCol_Est_1to5 Sebastien Carpentier KU Leuven massDryratioLeaf relativeLeaf_dry leafToPlantMassRatio_Comp_unitless Rhiannon Crichton Bioversity International LfEmRate_Comp_LfWk erect 5 Bioversity International CrmIntDiscFW_Est_1to5 Inge Van den Bergh 2 few internal spots Matooke firmness in mouth measurement scale 0-10 NARO, Uganda MatFirmM_Meas_0to10 Elizabeth Kakhasa obtuse and split 4 Rhiannon Crichton Bioversity International DIT_Comp_d temp11 Rhiannon Crichton Bioversity International Rhiannon Crichton PltPltd_Ct_Plnt Bioversity International DateHarv_Date_dmy Bioversity International Rhiannon Crichton numFuncLeaf numFuncLeaves_Count_unitless totalNumFunctionalLeaves Sebastien Carpentier KU Leuven Elizabeth Kakhasa NARO, Uganda Matooke aroma measurement scale 0-10 MatAro_Meas_0to10 FnctLeav_Ct_Leaf Rhiannon Crichton Bioversity International absent 0 IITA temp43 Tendo Ssali Guillaume Bauchet DamEst_Date_ymd IITA-BTI 3 long (equal to or more than 30 cm) Rhiannon Crichton BlkLfStrkLfFrStg1_Date_ymd Bioversity International Rhiannon Crichton DeathCause_Est Bioversity International 2 slightly angled 7 orange red 21-25 cm 3 Rhiannon Crichton PStmHt_M1_cm Bioversity International Rhiannon Crichton Bioversity International temp32 Bioversity International Rachel Chase BrctClExtFc_Est_1to10 5 plant dead, with brown leaves hanging down the pseudostem Rhiannon Crichton Bioversity International ChgNwLfPercFW_Comp_pct Bioversity International temp15 Rhiannon Crichton topLeafArea_Meas_cm2 Sebastien Carpentier area_topView areaPlantTop KU Leuven IITA GdFrtFll_Est_1to2 Allan Brown Rhiannon Crichton AvgFrtExtLng_M_cm Bioversity International 5 banana streak virus (BSV) massDryratioRoot relativeRoot_dry rootToPlantMassRatio_Comp_unitless Sebastien Carpentier KU Leuven 2 First streak stage: the initial speck elongates, becoming slightly wider, to form a characteristic narrow, reddish brown streak up to 20 mm long and 2.0 mm wide, with the long axis parallel to the leaf venation. At this stage, streaks are more clearly visible on the lower surface of the leaf than on the upper one. The distribution of streaks is variable. Sometimes they are most numerous near the edge of the left side of the leaf. At other times they are equally numerous on both sides of the leaf, and more or less evenly distributed. Frequently they are densely aggregated in a band several cm wide on one side or both sides of the mid-rib, becoming less numerous towards the edge of the leaf. Streaks may be so numerous that every cm2 of leaf surface bears one or more. There is considerable variation in the length of individual streaks on a given leaf, and they frequently overlap to form larger, compound streaks. 6 purple brown growthFresh plantGrowth_fresh Sebastien Carpentier KU Leuven growthFresh_Comp_g not dwarf 2 PdltFrt_Est_1to2 Allan Brown IITA Bioversity International/IITA Inge Van den Bergh/Danny Coyne NmNumRtWt wide with erect margins 2 smooth 10 PltNtHvstd_Ct_Plnt Bioversity International Rhiannon Crichton 1/3-2/3 discoloured 4 highly imbricate 3 2 small blotches no visual leaf symptoms 1 5 > 1/3 discoloured Third or mature spot stage: the centre of the spot dries out, becoming light grey or buff-coloured, and further depressed. The spot is surrounded by a narrow, well-defined, dark brown or black border. between the latter and the normal green colour of the leaf, there is often a bright yellow transitional zone. After the leaf has collapsed and withered, spots remain clearly visible because of the light-coloured centre and dark border. 6 SpotLfNum_Ct_Lf Amos Alakonya-Rhiannon Crichton IITA-Bioversity International MatGrass_Meas_2pt Matooke grassy aroma measurement 2pt scale NARO, Uganda Elizabeth Kakhasa Selection criteria for genotype that was ranked second lowest by male respondents during preference scoring exercise Noel Madalla Bioversity 10 high intensity Bioversity International/IITA Inge Van den Bergh/Danny Coyne temp46 Boiled plantain Mealiness scale 0-10 Hermann Antonin KOUASSI CIRAD / UNA, Côte d’Ivoire BoiPlantnMeal_Meas_0to10 other 5 Sebastien Carpentier KU Leuven plantFreshMass_Meas_g plant_fresh massFreshPlant Rhiannon Crichton MSuckN_Ct_MSuck Bioversity International Bioversity International Rhiannon Crichton BlkLfStrkLfFrStg6_Date_dmy massDryPlant Sebastien Carpentier KU Leuven plant_dry plantDryMass_Meas_g Bioversity International Rhiannon Crichton temp17 rwcRoot Sebastien Carpentier root_rwc KU Leuven rwcRoot_Comp_unitless FrtLngth_M_cm Rachel Chase Bioversity International pointed 1 IITA-Bioversity International BlkLfStrkSvrt_Est_pct RchsWt_M_kg Rhiannon Crichton Bioversity International 21-30 cm 2 3 straight in the distal part Rhiannon Crichton HndMltpFrtNum_Ct_Frt Bioversity International Allan Brown Sckr_Est_1to2 IITA intermediate 3 6 51-100% of lamina with symptoms Danny Coyne RadoNumSlWt_Comp_Nema IITA Rhiannon Crichton HndRnk_Ct_HndRnk Bioversity International IITA-BTI FngUnSmshdPlpTst_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) RkYgstLf_Comp_leafrank Bioversity International Rhiannon Crichton SSuckN_Ct_SSuck Bioversity International Rhiannon Crichton WvlLvPrTrp_Ct_weevil Tendo Ssali IITA present 2 Danny Coyne PratyNumSlWt_Comp_Nema IITA temp9 Bioversity International Rhiannon Crichton less than or equal to 2 m 1 6 Small bunch from neutral/hermaphrodite flowers just above the male bud Rhiannon Crichton AvgFrtGrth_M_mm Bioversity International Allan Brown/Guillaume Bauchet (temp.) IITA-BTI FngUnSmshdPlpCl_Est_1to5 Sebastien Carpentier waterLossSystem_per_Area KU Leuven waterLossSystPerLeafArea_Meas_mlcm2 Elizabeth Kakhasa MatSour_Meas_0to10 NARO, Uganda Matooke sourness measurement scale 0-10 5 Stage 5 is when the elliptical spot is totally black and has spread to the underside of the limb. It is surrounded by a yellow halo with the centre beginning to flatten out. FuncRtNum2_Ct_Rt Bioversity International/IITA Inge Van den Bergh/Danny Coyne Bioversity International DateDeath_Est_dmy Rhiannon Crichton 1 margins spreading horizontal or supra-horizontal 4 mole rats 8 BlkLfStrkSvrt_Est_Gauhl IITA-Bioversity International Bioversity International Rachel Chase MlBdShp_Comp_1to5 MeloiNumSlWt_Comp_Nema Danny Coyne IITA lanceolate 2 Bioversity International PStmCol_Est_1to16 Rachel Chase Rhiannon Crichton CropCyc_Comp_d Bioversity International Rhiannon Crichton temp19 Bioversity International BnchFrtNum_Comp_Frt Rhiannon Crichton Bioversity International Bioversity International Rhiannon Crichton AvgPeelThck_M_mm temp50 Bioversity International/IITA Inge Van den Bergh/Danny Coyne Rhiannon Crichton Bioversity International OffTypEst_Date_dmy Danny Coyne HoploNumSlWt_Comp_Nema IITA 3 34-66 % of older leaves turning yellow, with some hanging down the pseudostem Guillaume Bauchet FlwT_Date_ymd IITA-BTI HndNbWhlBnch_Ct_hands Bioversity International Rachel Chase FrtftPdclLgth_Est_1to3 Rachel Chase Bioversity International Inge Van den Bergh YellowPres_Est_1or2 Bioversity International 0 no egg masses FrtCvShp_Est_1to6 Bioversity International Rachel Chase IITA Dwfsm_Est_1to2 Allan Brown sparse blotching (<20%) 2 1 skinny (w/y less than or equal to 0.45) LfChgPres_Est_0or1 Bioversity International Inge Van den Bergh FlwT_Date_dmy Bioversity International Rhiannon Crichton Moist 10 CIRAD Christophe Jenny MlRchsTp_Estm_1to2 Rhiannon Crichton Bioversity International BlkLfStrkSvrtLfNb_Ct_grd1 Allan Brown/Guillaume Bauchet (temp.) FngSmshdPlpCl_Est_1to5 IITA-BTI Bioversity International Rhiannon Crichton NtHvstPrp_Comp_PlntNb temp41 Rhiannon Crichton Bioversity International Bioversity Selection criteria for genotype that was ranked third highest by male respondents during preference scoring exercise Noel Madalla Noel Madalla Bioversity PS_CompFemale_PrefScale MatMolT_Meas_0to10 Elizabeth Kakhasa NARO, Uganda Matooke moldability measurement scale 0-10 rootShootDry_Comp_unitless Sebastien Carpentier KU Leuven massDryRootShoot rootShootRatioDry Rhiannon Crichton Bioversity International FrtExtLng_M_mm 2 1-5% of lamina with symptoms 4 67-100 % of all leaves turning yellow and necrotic, with leaves hanging down the pseudostem Bioversity International WSuckN_Ct_WSuck Rhiannon Crichton 4 ovoid Rhiannon Crichton Bioversity International HvstPrp_Comp_PlntNb IITA Tendo Ssali AvgWvlLvBdWght_M_mg pink-purple 8 discolouration of between one-third and two-thirds of vascular tissue 4 11-30 egg masses 3 0 non sticky 2 neutral flowers on one to few hands only near the bunch (rest of stalk is bare) 5 > 75 % of roots galled Bioversity International Rhiannon Crichton FrtDiam_M_mm 2 dark brown 31-100 egg masses 4 IITA VitCCnt_M_ Allan Brown/Guillaume Bauchet (temp.) persistent style 2 BrctApxShp_Est_1to5 Bioversity International Rachel Chase Selection criteria for genotype that was ranked highest by male respondents during preference scoring exercise Noel Madalla Bioversity white 1 Bioversity International IntDisProp_Comp_pct Inge Van den Bergh heterogeneous 0 HandWt_M_kgPlnt Bioversity International Rhiannon Crichton 10 dont know 5 brown/rusty brown 1 less than or equal to 10 mm 1 bad Bioversity International Rhiannon Crichton BnchHndNum_Ct_Hnd 2 straight in the distal part 9 orange-red High shouldered (x/y less than or equal to 0.28) 1 Bioversity International FrtWt_M_gFruit Rhiannon Crichton Second-streak stage: streaks change colour from reddish brown to dark brown or black, sometimes with a purplish tinge, clearly visible at the upper surface of the leaf. 3 MlRchsPst_Estm_1to5 Rachel Chase Bioversity International Inge Van den Bergh/Danny Coyne temp51 Bioversity International/IITA slightly pointed 2 Noel Madalla Bioversity Selection criteria for genotype that was ranked third lowest by male respondents during preference scoring exercise temp30 Bioversity International Rhiannon Crichton 4 egg like very much 4 Danny Coyne IITA PratyGooNumSlWt_Comp_Nema FrtNb_Ct_gFruit Bioversity International Rhiannon Crichton IITA PratyCofNumSlWt_Comp_Nema Danny Coyne Rhiannon Crichton temp10 Bioversity International Noel Madalla Bioversity Selection criteria for genotype that was ranked third lowest by female respondents during preference scoring exercise Rhiannon Crichton LfEmRate_Comp_LfMth Bioversity International acronym no contrast between margin and petiole (without a colour line along) 1 without pigmentation 5 Rhiannon Crichton temp13 Bioversity International convolute 1 2 First streak stage: specks elongate, becoming slightly wider to form narrow reddish-brown streaks. Danny Coyne IITA HoploNumRtWt2_Comp_Nema Rachel Chase PMargWing_Est_1or5 Bioversity International Bioversity International Inge Van den Bergh YellowPres_Est_0or1 7 nematodes lumpy 0 rwcPlant Sebastien Carpentier KU Leuven rwcPlant_Comp_unitless plant_rwc 5 not winged and not clasping the pseudostem Rhiannon Crichton AnPltPrp_Comp_KgHaY Bioversity International 9 whitish Amos Alakonya-Rhiannon Crichton SptLfNb_Ct_leaf IITA-Bioversity International IITA-BTI DateHarv_Date_ymd Guillaume Bauchet KU Leuven leafChlorosis_Comp_unitless leafChlorosis Sebastien Carpentier 1 dislike very much temp35 Rhiannon Crichton Bioversity International Sebastien Carpentier KU Leuven pseudostemHeightIncrease pseudostemHeightIncrease_Comp_cm2 0 no intensity AnthrCl_Est_1to8 Bioversity International Rachel Chase 6-15% of lamina with symptoms 3 Selection criteria for genotype that was ranked second highest by male respondents during preference scoring exercise Noel Madalla Bioversity green yellow 4 4 grey medium intensity 5 male flowers/bracts above the male bud (rest of stalk is bare) 3 Matooke hardness in hand measurement scale 0-10 Elizabeth Kakhasa MatHardT_Meas_0to10 NARO, Uganda BlkLfStrkLfFrStg6_Date_ymd Rhiannon Crichton Bioversity International 0 no intensity Stage 1 appears as a small depigmentation spot whose whitish or yellow colour resembles stage 1 of Yellow Sigatoka disease. These symptoms are not visible in transmitted light and can be observed only on the underside of the leaf. 1 10 pink/pink-purple Bioversity International Rhiannon Crichton temp23 at an angle 2 temp18 Bioversity International Rhiannon Crichton 1 like a top weevils 6 Rhiannon Crichton PltEst_Date_dmy Bioversity International IITA Tendo Ssali AvgWvlAdltHdCps_M_µM IITA-Bioversity International BlkLfStrkSvrt_Est_MedLawCdsd 2 0-33 % of older banana leaves turning yellow Second-streak stage: the streaks may elongate slightly, but the most notable change is that in colour from reddish brown to dark brown or almost black, sometimes with a purplish tinge. The streak is now clearly visible on the upper surface of the leaf. The distribution of streaks at this stage of development varies in the same way as mentioned above. When streaks are very numerous, and more or less evenly distributed, the entire leaf blackens. 3 less than or equal to 12 1 Bioversity International temp14 Rhiannon Crichton straight (or slightly curved) 1 IITA-Bioversity International BlkLfStrkSvrt_Est_MedLawCpt orange-brown (mahogany, like in Pisang Mas) 1 entity 3 equal to or more than 3 m 4 spiral (all fruit are attached to a unique crown coiled around the stalk) margins curved inward 3 Sebastien Carpentier KU Leuven root_dry rootDryMass_Meas_g massDryRoot Bioversity International HvstDst_Comp_PlntHa Rhiannon Crichton Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd2 Bioversity International rounded 5 straight with erect margins 3 AnCrpCyclPrp_Comp_CrpCclY Bioversity International Rhiannon Crichton 3 intermediate medium green 6 ExtDisProp_Comp_pct Inge Van den Bergh Bioversity International FrtNbMdHndBnch_Ct_ft Bioversity International Rachel Chase Inge Van den Bergh Bioversity International CormDiscol_Est_1to6 Bioversity International Rachel Chase CmpdTplMnCl_Est_1to16 Stage 3 differs from the previous one by its dimensions. The stripe becomes longer, is enlarged and in certain conditions (weak inoculum and unfavourable climatic conditions) can reach 2 or 3 cm. 3 Shoot_Date_ymd IITA-BTI Guillaume Bauchet 4 yellow singleLeafWidth_Meas_cm widthPerLeaf widthSingleLeaf Sebastien Carpentier KU Leuven like extremely 5 2 brown 2 adult female Bioversity International PltDnsty_Comp_PlntHa Rhiannon Crichton Bioversity International BnchShp_Estm_1to5 Rachel Chase Rhiannon Crichton NtHvstDst_Comp_PlntHa Bioversity International Inge Van den Bergh CrmDiscDistTlstSckFW_M_cm Bioversity International 1 hanging vertically 3 banana Xanthomonas wilt (BXW) equal to or more than 31 cm 3 Second-spot stage: the dark brown or black central area of the spot becomes slightly depressed and the water-soaked border becomes more pronounced due to darkening. At this stage, a slight yellowing of the leaf tissue immediately surrounding the water-soaked border may occur. 5 3-10 egg masses 2 MatPump_Meas_2pt NARO, Uganda Matooke pumpkin aroma measurement 2pt scale Elizabeth Kakhasa Bioversity International BnchFrtNum_Ct_Frt Rhiannon Crichton Tendo Ssali WvlAdltPrTrp_Ct_weevil IITA curved in slight S shape (double curvature) 5 Bioversity International Rachel Chase MgnBhvPtCnlThrdLf_Est_1to4 leaf spots 1 Rhiannon Crichton Bioversity International BlkLfStrkSvrtLfNb_Ct_grd4 2 2.1-2.9 m 3 totally fused (more than 50 % of the length of the pedicel) Sebastien Carpentier leavesFormedPerPseudostemGrowth_Comp_percm KU Leuven leaves_per_heightPS not winged 2 3 hanging at a 45 degree angle with a curve 3 2 truncate ( 4 16-33% of lamina with symptoms 5 light green ** 2 CigLfUnrBrnStgB_Date_dmy Bioversity International Rhiannon Crichton Elizabeth Kakhasa Matooke smoothness in hand measurement scale 0-10 NARO, Uganda MatSmoothM_Meas_0to10 leafDryMass_Meas_g massDryLeaf leaf_dry Sebastien Carpentier KU Leuven IITA Tendo Ssali WvlLvMsrd_Ct_weevil leafAreaIncrease leafAreaIncrease_Comp_cm2 Sebastien Carpentier KU Leuven high intensity 10 temp20 Bioversity International Rhiannon Crichton 0 no symptoms areaSingleLeaf Sebastien Carpentier areaPerLeaf KU Leuven singleLeafArea_Meas_cm2 attribute other 8 cream 2 6 orange Rhiannon Crichton Bioversity International BlkLfStrkInfIndex_Comp_Idx blue 7 5 grainy 4 black-purple Bioversity International PulpDiam_M_mm Rhiannon Crichton 2 dont know 3 equal to or more than 21 mm 5 Second-spot stage: the dark brown or black centre of each lesion becomes slightly depressed and water soaked border becomes more pronounced. 2 partially fused (up to 50 % of the length of the pedicel) Bioversity International Rhiannon Crichton PlntShtTime_Comp_d Banana ontology Plantain ontology 5 obtuse and split Noel Madalla Bioversity Selection criteria for genotype that was ranked second highest by female respondents during preference scoring exercise 1 without flower relicts Bioversity International FngWtTtl_Comp_kg Rhiannon Crichton 3 ivory Tendo Ssali IITA WvlLvBdLgt_M_µM 4 Stage 4 appears on the underside as a brown spot and on the upper side as a black spot. Rachel Chase Bioversity International PMargWing_Est_1or2 1 present Bioversity International PStmGrth_M_cm Rhiannon Crichton base of the style prominent 3 Allan Brown/Guillaume Bauchet (temp.) IITA-BTI FngSmshdPlpTst_Est_1to5 5 discolouration greater than two-thirds of vascular tissue 0 absent homogeneous 10 1 add** 3 black-purple 1 present 3 juvenile pink/pink-purple 6 clasping 1 absent 1 Bioversity International HndFrtNum_Ct_Frt Rhiannon Crichton 3 obtuse 1 sparse blotching one yellow finger 2 3 discolouration of up to one-third of vascular tissue truncated 1 2 11-20 mm no pigmentation 1 other 5 yellow 2 10 firm 2 contrast between margin and petiole (with a contrasting colour line along) DthDnst_Comp_PlntHa Rhiannon Crichton Bioversity International not winged and clasping the pseudostem 4 3 persistent flower relicts (> 20% of the fruits with relicts) RtnCrpCyc_Comp_d Rhiannon Crichton Bioversity International Stage 6 is when the centre of the spot dries out, turns clear gray and is surrounded by a well-defined black ring, which is, in turn, surrounded by a bright yellow halo. These spots remain visible after the leaf has dried out because the ring persists. 6 10 high intensity 5 erect total discolouration of vascular tissue 6 Bioversity PS_CompMale_PrefScale Noel Madalla 50-75 % of roots galled 4 not clasping 2 3 25-50 % of roots galled falling vertically 1 3 moderate blotching (20-50%) trace infections with a few small galls 1 neutral/male flowers on the whole stalk without persistent bracts (still firmly attached to the rachis) 5 7 pink-purple dwarf 1 2 medium (0.45 < w/y < 0.55) medium shouldered (0.28 < x/y < 0.30) 2 1 winged and undulating Bioversity International Rachel Chase BrctClExtFc_Int_1to16 extensive pigmentation (>50%) 4 Bioversity International OdStdLfRk_Ct_leaf Rhiannon Crichton watery green 3 10 high intensity 7 green 5 medium intensity short (y less than or equal to 20 cm) 1 light green 5 5 bright yellow 3 purple to blue 10 sticky 4 neutral/male flowers and presence of withered bracts on the entire stalk Rachel Chase AnthrCl_Est_1to16 Bioversity International pseudostemToPlantMassRatio_Comp_unitless massDryratioPseudostem relativePS_dry Sebastien Carpentier KU Leuven Rhiannon Crichton Bioversity International AcAnYd_Comp_TnHaY curved in S shape (double curvature) 4 very light yellow 0 equal to or more than 31 cm 5 9 whitish Dry 0 34-50% of lamina with symptoms 5 obtuse 4 3 like fairly 6 purple fully green 1 medium intensity 5 4 only base of the style persists 5 Other 1 without any floral relicts Bioversity International Rachel Chase FrtLngth_M_1to5 red 3 ShtHvstTime_Comp_d Bioversity International Rhiannon Crichton 1 bare corm completely clean, no vascular discolouration 1 less than 1% of lamina with symptoms, only streaks and/or up to 10 spots 1 green yellow 4 2 not parthenocarpic dark green 8 Initial speck stage: symptoms are first visible to the naked eye as faint, minute (less than 0.25 mm diam), reddish brown specks on the lower surface of the leaf. Specks are often most abundant near the margin of the left side of the leaf, particularly towards the tip. Where leaf spot is severe, specks have been observed on the second leaf of plants that have not yet produced a bunch. Elsewhere they usually appear on the third, fourth, or older leaves. 1 1 cylindrical 2 margins erect 2 present 1 adult male green 1 2 slightly curved medium intensity 5 other 6 medium green 6 soft 0 hard 10 2 good 5 purple-brown 3 equal to or more than 17 horizontal 4 other 4 4 margins overlapping 3 winged and clasping the pseudostem 2 moderately imbricate pink-purple to red 2 parthenocarpic 1 < 25 % of roots galled 2 brown-black 3 1 white 4 banana bunchy top (BBTD) Third or mature spot stage: the centres of the lesions dry out becoming light grey or buff coloured and a bright yellow transitional zone appears between them and the normal green colour of the leaf. The lesions remain clearly visible after the leaf has collapsed or withered because of their light coloured centre and dark border. 6 dark green 8 other 4 margins curved inward 4 crumbly 0 1 straight winged 1 2 not revolute (not rolling) 0 no intensity 1 cream 1 green entire inner rhizome discoloured 6 9 red-purple 5 rounded 2 Stage 2 appears as a stripe, generally brown in colour and visible on the underside of the leaf, later the symptom also appears on the upper part of the limb as a stripe, the yellow colour of which resembles the stripe at stage 1 of Yellow Sigatoka. The colour of this stripe will change progressively from brown and later to black on the upper side of the limb, but will retain the brown colour on the underside. cream 2 3 blunt-tipped (plateau at tip) Time from shooting to harvest Time from shooting to harvest The time elapsed from shooting (when the inflorescence emerges from the pseudostem and is still in an erect position) to the harvest of the bunch. shoot2harvest Banana weevil larvae head capsule width The width of a weevil larvae head capsule. Head capsule width Visually observe the last bract to lift from the hands, ideally when the bract has lifted to a horizontal position, and choose the option that is most similar, from the 2 photos of descriptpr 6.5.12 in the reference material. Bract behaviour before falling - estimation blotch pigmentation scale (1 to 5) Combined proportion of plants that are dead deathprop The proportion of plants that are dead, from the number of plants planted. Death proportion Calculate as: the length of a year (e.g. 365 days) divided by the Plant crop cycle or the Ratoon crop cycle, e.g. 365 / 334 = 1.09. Annual crop cycle proportion - computation Length Length of the fruit pedicel from rachis to the beginning of the pulp Fruit pedicel length Potential annual yield - computation Calculate as: the Bunch weight divided by 1,000, multiplied by the Annual crop cycle proportion, multiplied by the Plant density, e.g. (50 / 1,000) * 1.09 * 1,667 = 90.85. The proportion of plants that are alive but not harvested (e.g. due to slow or poor development), from the number of plants planted. Non-harvest proportion Combined proportion of plants not harvested nonharvprop Calculate as: the Number of functional roots divided by the Number of all roots, multiplied by 100, e.g. 12 / 20 * 100 = 60 % functional roots. Percentage of functional roots - computation firmness scale 0-10 Record the date of event. Date of damage - estimation mg Length [x at maturity] Length of the internal arc of a finger ** subsume Fruit length Measurement under microscope Weevil larvae head capsule - measurement BD colour chart A - 2016 leaf Number of fingers measured - counting Count how many fingers are used to obtain a measurement. Genotype selection traits Description of traits considered important in selection of third least genotype by respondents Selection criteria for genotype ranked third lowest by respondents Presence absence 0 Calculate as: the Number of plants planted, minus the Number of plants dead, minus the Number of plants harvested, e.g. 24 - 4 - 18 = 2. Number of plants not harvested - computation The smoothness-mouth feel of the smashed pulp of a mature fruit Fruit smashed pulp smoothness-mouth feel Smoothness-mouth feel Time from harvest to death Time from harvest to death harvest2death The time elapsed from the harvest of the bunch of the plant from the preceding crop cycle to the death of the plant from the current crop cycle. Presence Presence or absence of colour line along edge of petiole margin Colour line along edge of petiole margin mL / cm? % open answer None Index of black leaf streak disease severity Average finger weight - computation Calculate as: the sum of the the Finger weight measurements, divided by the number of those measurements, e.g. (150 + 146 + 147 + 152 + 140 + 146) / 6 = 147. waterLossDay Daily rate of water loss The average daily water loss Rate Time elapsed from Bruns stage B of unrolling to Foures black leaf streak disease severity scale 6 DDT The time elapsed between a tagged cigar leaf at Bruns stage B of unrolling and the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6. Black leaf streak disease development time Weigh multiple fingers together, using scales. Associate the data with the Hank rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Multiple finger weight - measurement % Dry weight - measurement 80?C, for 14 days, or longer untill dry Fruit pulp lateral diameter - measurement Cut a finger in half, crosswise at its widest point, and on the cross-section measure the lateral diameter of the fruit pulp, from the left to the right side (not from the ventral to the dorsal side), using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Date of weevil damage to corm assessment - estimation Record the date of event. Measure the circumference of the pseudostem of the plant at 75 cm from the collar, or from the pseudostem base at the ground if the collar is not visible, using a tape measure. Plant circumference 75 cm - measurement Focus group discussion for second lowest ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked second lowest in preference ranking exercise Record the date and time of event. End date and time of data collection - estimation Leaf trait correlated to the health stage of the leaf leafChlorosis Leaf chlorosis Chlorosis Nematode egg-laying females extent - estimation Collect all roots from a standard size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Carefully wash the soil from the roots with tap water. Cut the roots into 1 cm pieces. Take a subsample of 5 g, add 100 ml distilled water and store the roots at 4 degrees Celsius. Stain the egg masses by immersing the roots in 0.15 g/L phloxine B for 15 mins. Count the number of egg-laying females under a stereo microscope. numfingersouterwhorl2nddistalhand Number of fingers in outer whorl of 2nd-most distal hand Number of fingers in outer whorl The number of fingers in the outer whorl of the second-most distal hand []/cm bunch shape scale (1 to 5) 1 to 5 acceptability score End date and time of data collection Date and time of end of data collection The date and time of the end of data collection on a plant. dateendtimedatacoll Average thickness of the peel avepeelthickness Average finger peel thickness The average thickness of the peel of a finger. Number of leaves with a black leaf streak disease severity grade of 0 The number of leaves on a plant with a black leaf streak disease severity grade of 0. Number of leaves with black leaf streak disease severity grade 0 Damage percent X-upper-outer The amount of damage to the outer corm (cortex) upper cross-section, assessed by percentage of tunnels caused by weevils. Percentage damage to upper cross-section outer corm caused by weevils Date of flowering dateflower The date the last bracts fall from the most distal hand (closest to the male bud) to display the female flowers. Date of flowering dd/mm/yyyy Black leaf streak disease development time - computation Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 and the Date of tagged cigar leaf unrolling at stage B, e.g. 24/06/2014 - 24/03/2014 = 92 days. Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Record on the last developed leaf at [x flowering stage] [shooting]. Colour line along edge of petiole margin - estimation Record the date of event. Date of flowering - estimation Root knot nematode galling Focus group discussion for second lowest ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was second ranked lowest in preference ranking exercise Distance of discolouration The distance of discolouration in the pseudostem of the plant, caused by Fusarium wilt. Distance of internal discolouration in pseudostem of plant caused by Fusarium wilt Vitamin C content The Vitamin C content of freeze-dried smashed pulp of a mature fruit Vitamin C content Calculate as: the time elapsed between the Date of shooting to the Date of harvest. E.g. 24/12/2014 - 24/09/2014 = 91 days. Time from shooting to harvest - computation Visually observe the fusion of pedicels and choose the option that is most similar, from the 3 photos of descriptor 6.7.11. in the reference materials. Observe before they join the rachis at the bract scar. Look upwards from the bottom of the bunch. Fusion of pedicels - estimation Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. Count the number of dead roots. Number of dead roots - counting Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Radopholus similis ) per unit fresh root weight -method The average weight of the fingers. avefingerweight Average weight Average finger weight Time from flowering to harvest - computation Calculate as: the time elapsed between the Date of flowering to the Date of harvest, e.g. 24/12/2014 - 24/10/2014 = 61 days. Male bud length Length of male bud from end of rachis to apex of the bud Length Average number of banana weevil adults The average of how many adult banana weevils caught per trap. Average number of banana weevil adults per trap Number of Pratylenchus goodeyi in soil Population density Number of Pratylenchus goodeyi per soil sample bud shoulder scale (1 to 3) Visual assessment. Presence of splitting pseudostem base caused by Fusarium wilt - estimation Cut a hand from the rachis and weigh the hand, using scales. Associate the data with the Hand rank. Hand weight - measurement Manual height measurement Length - measurement Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked highest in preference ranking exercise Focus group discussion for top traits with male respondents Number of fingers in 2nd-most distal hand - counting Count how many fingers are in the second-most distal hand. Plant crop cycle plant2harvest The time elapsed from planting to the harvest of the bunch. Time from planting to harvest maiden sucker leaf rank Blotches area - estimation Record the relative surface area coverage by blotches. [x Look at several plants if possible to get an overall idea. Observe at flowering time]. Finger feel assessment of smashed and unsmashed fruit pulp Finger feel - estimation Calculate as: the Plant density multiplied by the Non-harvest proportion, e.g. 1,667 * 0.08 = 133. Non-harvest density - computation nematodes/unit of fresh root weight The weight of multiple hands. Multiple hand weight multhandweight Weight mL Put a part of the product and by retro-olfaction evaluate the presence and the intensity of matoke-like aroma Matooke-like Aroma method plants dead/plants planted Monthly leaf emission rate LER Monthly leaf emission rate A rate that expresses the number of leaves that have emerged from the pseudostem in a month. day cm Number of leaves with a black leaf streak disease severity grade of 1 Number of leaves with black leaf streak disease severity grade 1 The number of leaves on a plant with a black leaf streak disease severity grade of 1. kg/plant/y Tallest sucker height - measurement On the tallest sucker, measure the distance from the pseudostem base at the ground to the intersection of the petioles of the two youngest leaves (leaf ranks 1 and 2), using a measuring pole or sliding ruler. Number of nematodes (Meloidogyne spp.) per unit fresh root weight - method Count from 100 ml of soil sample multiplication factor Nematode species - estimation Identify the nematode species present in the banana plant root sample (Radopholus similis, Pratylenchus coffeae, Pratylenchus goodeyi, Helicotylenchus multicinctus, Meloidogyne incognita, ?) The number of leaves on the plant that have 50 % or more of the leaf surface area as green, healthy, photosynthetic tissue. Number of functional leaves functleaves Number of functional leaves Leaf width - measurement The shortest diagonal of a fitted ellips Ratio - computation Ratio of root dry mass versus plant dry mass bract behaviour scale (1 to 2) The height of the pseudostem of the plant, from the collar to the intersection of the petioles of the two youngest leaves. Plant height plantheightpetioles Height The width of a leaf Width Single leaf width singleLeafWidth Pest and/or disease assessment and/or sample collection options pestdiseasesampleoptions Pest/disease assessment/sample collection options The options to record the pest and/or disease assessment and/or sample collection done at the time of data collection. Apex point Fruit apex point Description of fruit apex blotch color scale (1 to 5) Selection criteria for genotype ranked third highest by respondents Genotype selection traits Description of traits considered important in selection of third best genotype by respondents Angle of the rachis between last hand and male bud Angle of the part of the rachis between the last hand and the male bud Male rachis position The date of death of the plant. Date of death datedeath Date of death hands Growth - computation Dry weight difference between timepoints (refer to experimental metadata) Number of hands in bunch - counting Count how many hands are in the bunch. bract apex scale (1 to 4) functional roots PC Presence of petiole collapse caused by Fusarium wilt The presence/absence of petiole collapse, caused by Fusarium wilt. Presence/absence of collapse Calculate as: the Rank of previously marked leaf at one point in time, minus 1 (equivalent to the Rank of marked youngest leaf), divided by the time elapsed between the two Date of data collection events when the marked leaf was recorded as 1) the Rank of marked youngest leaf and 2) the Rank of previously marked leaf, e.g. (4 - 1) / 1 month = LER of 3 leaves/month during a particular time period. Monthly leaf emission rate - computation Fresh weight - measurement Weighing Record the date of event. Date of first external disease symptoms caused by Fusarium wilt - estimation Pest and/or disease assessment and/or sample collection description - estimation Describe the pest and/or disease assessment and/or sample collection taking place at the time of data collection. Dry weight The dry weight of a leaf leafDryMass Leaf dry weight The average length of the fingers measured along the external (dorsal) arc, excluding the pedicel and the fruit tip. avefingerextlength Average finger external length Average external length Firmness Boiled plantain Boiled plantain Firmness BoiPlantnFirm Force required to obtain deformation, penetration or rupture of the banana plantcircumf75cm Plant circumference 75 cm from the collar Circumference 75 cm above the ground The circumference of the pseudostem of the plant at 75 cm from the collar. Leaf area - measurement Top view image of a single leave Matooke Moldability in hand Matooke moldability mechanical textural attribute relating to the degree to which a substance can be deformed before it breaks MatMolT Measure the length of the internal arc of a fruit, without pedicel. Record on the inner fruit in the middle of the mid-hand of the bunch. If there is an even number of hands, there will be two middle hands so use the upper hand that developed first. Record the exact value. Fruit length - exact value - measurement MatStickT Stickiness in hand Matooke stickiness mechanical textural attribute relating to the force required to remove material that sticks to the mouth Matooke Combined number of plants that are dead The number of plants that are dead. numdead Number of plants dead Population density Number of nematodes in soil Nematode population density by species in soil Male rachis position - estimation Visually observe the male rachis position (the part between the last hand and the male bud) and choose the option that is most similar, from the 5 schematic drawings of descriptor 6.4.12 in the reference material. Visually observe the remains, or not, of flower relicts at the finger apex, and choose the option that is most similar, from the 4 photos of descriptor 6.7.7 in the reference materials. Remains of flower relicts at fruit apex - estimation Multiple number of fingers in hand - counting Count how many fingers are in multiple hands. Associate the data with the Hand rank and the Number of hands measured. The recommendation is to collect this data from the second and second-most distal hands. Number of maiden suckers - counting Count how many maiden suckers are in the mat. Water loss rate - computation Measurement of system water loss between two timepoints (refer to experimental metadata) Number of hands Number of hands on bunch **subsume Number of hands on whole bunch The time elapsed from the harvest of the bunch of the plant from the preceding crop cycle to the harvest of the bunch of the plant from the current crop cycle. harvest2harvest Ratoon crop cycle Ratoon crop cycle Count how many functional leaves (leaves that have 50% or more of their surface as green, healthy, photosynthetic tissue) are on the plant. Number of functional leaves - counting Calculate as: the Bunch weight, minus the Rachis weight, e.g. 50 - 10 = 40. Total finger weight - computation Calculate as: the sum of the number of values for Date of death. Number of plants dead - computation The length of a banana weevil adult body, from the head to the tip of the abdomen. Banana weevil adult body length Body length Damage to upper inner corm weevils - measurement Cut a transverse cross section of the corm at the collar (upper cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Visually observe the colour of the external face of the first unlifted bract. Use colour chart A and observe out of direct sunlight. Colour of bract external face - estimation Calculate as: the sum of values with a Black leaf streak disease severity grade of 5. Number of leaves with black leaf streak disease severity grade 5 - computation Calculate as: the sum of the number of values for Presence of splitting pseudostem base caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 24 / 300 * 100 = 8%. Percentage of plants with a splitting pseudostem base caused by Fusarium wilt - computation The dry weight of the root system Root dry weight Dry weight rootDryMass Ratio rootShootDry The ratio of dry mass of belowground (root) to abovegrund (shoot) biomass Ratio root to shoot Pest and/or disease assessment and/or sample collection options - estimation Record the pest and/or disease assessment and/or sample collection taking place at the time of data collection. Measure the length of a finger, along the internal (ventral) arc, excluding the pedicel and the fruit tip, using a tape measure. Associate the data with the Hand rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger internal length - measurement Date of shooting - estimation Record the date of event. avenumfingshand Average number of fingers in hand The average number of fingers in a hand. Average number of fingers Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 The date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6. Date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6 A description of the damage to the plant. describedamage Damage description Description of damage Subjective taste assessment of smashed and unsmashed fruit pulp Taste - estimation Number of hands in bunch numhands Number of hands The number of hands in the bunch. Number The number of functional roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Number of functional roots Rank of marked youngest leaf rankyoungleaf Rank The rank (position) of the youngest completely unrolled leaf. Preference number scale mm avefingerlatdiam Average lateral diameter Average finger lateral diameter The average lateral (side to side) diameter of a finger. Extent of internal discolouration in corm of plant caused by Fusarium wilt Extent of discolouration The extent of discolouration in the corm of the plant, caused by Fusarium wilt. Date of death - estimation Record the date of event. The total number of all roots (both dead and functional) in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Number of all roots Number Calculate as: the sum of values with a Black leaf streak disease severity grade of 1. Number of leaves with black leaf streak disease severity grade 1 - computation Start date and time of data collection - estimation Record the date and time of event. finger An index to express the proportion of standing leaves without the typical late-stage symptoms of black leaf streak disease, i.e. a black spot with a necrotic centre. This index provides an estimation of available photosynthetic leaf area prior to fruit filling and is a measure of resistance. It also corrects for the difference in the number of leaves produced by different types of bananas. Index of non-spotted leaves Index of non-spotted leaves INSL Number of sword suckers - counting Count how many sword suckers are in the mat. (Leaf Fresh Weight - Leaf Dry Weight) / Leaf Fresh Weight Water content - computation Average body length Banana weevil adult average body length The average length of a banana weevil adult body, from the head to the tip of the abdomen. Grassy-like Aroma method Put a part of the product and by retro-olfaction evaluate the presence and the intensity of grassy-like aroma Banana weevil adult average head capsule width Average head capsule width The average width of a banana weevil adult head. Calculate as: the time elapsed between the Date of planting and the Date of first youngest leaf spotted, e.g. 24/06/2014 - 24/01/2014 = 151 days. Time from planting to first youngest spotted leaf - computation Count how many fingers are in a hand. Associate the data with the Hand rank. The recommendation is to collect this data from the second and second-most distal hands. Number of fingers in hand - counting Date and time of start of data collection datestarttimedatacoll Start date and time of data collection The date and time of the start of data collection on a plant. Calculate as: the sum of the number of values for Presence of changes in new leaves caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 67 / 300 * 100 = 22%. Percentage of plants with changes in new leaves caused by Fusarium wilt - computation Matooke Matooke aroma grassy Aroma of fresh grass MatAroGrassA Grassy-like aroma Finger weight - measurement Weigh a finger, using scales. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. The average of how many banana weevil larvae caught per trap. Average number of banana weevil larvae per trap Average number of banana weevil larvae dateshoot Date of shooting Date of shooting The date the inflorescence emerges from the pseudostem and is still in an erect position. Calculate as: 100 multiplied by (Youngest leaf spotted, minus 1), divided by Number of standing leaves, e.g. 100 * (6 - 1) / 12 = 42. Index of non-spotted leaves - computation kg Visual estimation of suckering, for Early Evaluation Trial stage only. Sucker quality - estimation Number of Radopholus similis in roots Number of Radopholus similis per fresh root weight Population density Taste method Sweetness Put a piece of banana in your mouth, chew it and swirl it around your tongue to detect the sweet flavor Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Pratylenchus coffeae) per unit fresh root weight -method Chewiness Sensory Texture method Place the sample in the mouth, chew it at the rate of one chewing per second and assess the number of chews before swallowing. State description statedescription A description of the state of the plant. Plant state description Count how many fingers are in the third hand. Number of fingers in 3rd hand - counting Number of nematodes (Pratylenchus coffeae) per unit fresh root weight - method Count from 100 ml of soil sample unitless Extent of root knot nematodes Nematode egg-laying females extent The extent of egg-laying female root knot nematodes, observed internally as pit-like structures on the roots. Record the date of event. Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 - estimation The dry weight of the pseudostem Pseudostem dry weight Dry weight pseudostemDryMass g Measure the lateral diameter of a finger from the left to the right side (not from the ventral to the dorsal side), at the widest point, using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger lateral diameter - measurement Stickiness scale 0-10 plants/unit of area Bunch shape - estimation Visually observe the shape of the mature bunch on a fully developed plant that is not experiencing environmental stress and choose the option that is most similar, from the 4 photos of descriptor 6.4.7. in the reference material. How many banana weevil larvae caught per trap. Number of banana weevil larvae Number of banana weevil larvae per trap Hand weight The weight of a hand. handweight Weight Firmness scale 0-10 Damage to lower inner corm weevils - measurement Cut a transverse cross section of the corm at 10 cm below the collar (lower cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Number of fingers in 3rd hand The number of fingers in the third hand. numfingers3rdhand Number of fingers Ratio Measure for the biomass allocation: dry mass of leaves versus whole plant dry mass ratio Leaf to whole plant mass ratio leafToPlantMassRatio_dry suckering Overall suckering quality The overall estimation of sucker quality on a mat Sucker quality Visual estimation of a fruit fill, (or parthenocarpic) for Early Evaluation Trial stage only. Fruit parthenocarpy - estimation Calculate as: the Total finger weight, divided by the Number of hands in bunch, e.g. 46.8 / 16 = 2.9. Average hand weight - all - computation Leaf area increase between timepoints (refer to experimental metadata) per number of new leaves formed between timepoints (refer to experimental metadata) Growth - computation Ratio of non green to green plant pixels Leaf chlorosis - computation Anther colour Main colour of the face of anther opposite to dehiscence Colour Fresh weight - measurement Weighing Put a part of the sample between thumb and index fingers and using tapping motions, evaluate the amount of product adhering on them Stickiness in hand method Visually observe the angle of the bunch and choose the option that is most similar, from the 5 schematic drawings of descriptor 6.4.6 in the reference material. Bunch position - estimation Weighing Fresh weight - measurement The percentage of the root cortex of five functional roots approximately 10 cm long that is necrotic due to nematode damage. Root necrosis caused by nematodes Percentage necrotic Description of traits considered important in selection of second best genotype by respondents Selection criteria for genotype ranked second highest by respondents Genotype selection traits Leaf area to plant area ratio Ratio leafAreaRatio Ratio of projected leaf area to the actual leaf area, measure for the compactness of the plant crown Fresh plant growth growthFresh Fresh mass accumulation over time Growth Fresh weight pseudostemFreshMass Pseudostem fresh weight A pseudostem weight trait which is the fresh weight of a pseudostem Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Observation should be made at shooting on the neck, where the petiole and pseudostem meet. Margin is the part of the petiole that can be bent outwards/inwards. [x Observe at flowering time.] Petiole margin clasping - estimation Number of banana weevil traps used How many How many banana weevil traps used. Record the rank (order) of the youngest leaf spotted (the first fully unfurled leaf with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres), counting the rank by starting with the youngest completely unrolled leaf as 1 and moving downwards, associating the data with the Date of data collection. Youngest leaf spotted - counting potannyield The average potential annual yield, assuming that all plants are harvested, from the number of plants planted. Average potential annual yield Average potential annual yield Damage percent X-lower-inner Percentage damage to lower cross-section inner corm caused by weevils The amount of damage to the inner corm (central cylinder) lower cross-section, assessed by percentage of tunnels caused by weevils. Annual yield Plant annual yield The bunch weight produced by the plant over the course of the crop cycle, averaged across one year. plantannyield Texture The texture of the unsmashed pulp of a mature fruit Fruit unsmashed pulp texture dwarfism Non-harvest proportion - computation Calculate as: the Number of plants not harvested, divided by the Number of plants planted, e.g. 2 / 24 = 0.08. Calculate as: the time elapsed between the Date of planting and the Date of death, e.g. 24/05/2014 - 24/01/2014 = 120 days. Time from planting to death - computation Compound tepal main colour - estimation The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the colour of the backside middle of tepal. Use colour chart B and observe out of direct sunlight. bud shape scale (1 to 3) Combined density of plants not harvested nonharvdens The number of plants that are alive but not harvested (e.g. due to slow or poor development), in a defined area. Non-harvest density Record the date of event. Date of planting - estimation Firmness in mouth method Put a part of the sample in your mouth, evaluate during the first bite (between molars) how hard is the sample. Matooke Aroma of the local matooke. MatAroMatA Matooke aroma matooke Matooke-like aroma Body weight Banana weevil adult body weight The weight of a banana weevil adult weevil body. Black leaf streak disease evolution time - computation Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 and the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1, e.g. 24/06/2014 - 24/04/2014 = 61 days. Damage percent X-lower-outer The amount of damage to the outer corm (cortex) lower cross-section, assessed by percentage of tunnels caused by weevils. Percentage damage to lower cross-section outer corm caused by weevils Efficiency The growth (weight increase) per volume transpired water Transpiration efficiency transpirationEfficiency Put a part of the product and by retro-olfaction evaluate the presence and the intensity of pumpkin-like aroma Pumpkin-like Aroma method Population density Number of Rotylenchulus spp.per soil sample Number of Rotylenchulus spp.in soil Leaf area ratio - computation Computation of top leaf area with total (loose) leaf area Cause of damage The apparent cause of damage to the plant. Cause of damage causedamage dd/mm/yyyy hh:mm:ss The date the assessment of weevil damage to the corm of the plant is conducted. Date of weevil damage to corm assessment Date of weevil damage assessment kg Number of adult weevils measured How many banana weevil adults used to obtain a measurement. Number of banana weevil adults measured Hand rank of 2nd-most distal hand Rank The rank (order) of a the second-most distal hand in the bunch. handid2ndmostdistal rachisweight Weight Rachis weight The weight of the rachis. plant Record the rank (order) of the oldest standing leaf (with an erect petiole), counting the rank by starting with the youngest completely unrolled leaf as 1 and moving downwards, associating the data with the Date of data collection. Rank of oldest standing leaf - counting Percentage of plants with external disease symptoms caused by Fusarium wilt - computation Calculate as: the sum of the number of plants with any or multiple of the external disease symptoms caused by Fusarium wilt - yellowing leaves, splitting of pseudostem base, changes in new leaves, petiole collapse - divided by the Number of plants planted * 100, e.g. 112 / 300 * 100 = 37%. Calculation of preference score by respondents Overall plant appearance PS Preference score Banana weevil larvae average body length The average length of a weevil larvae body, from the head to the tip of the abdomen. Average body length The current rank (position) of the leaf that was previously marked as the youngest leaf at the time of the last data collection event. Rank rankprevleaf Rank of previously marked leaf Cause of damage - estimation Record the apparent cause of the damage to the plant, e.g. strong winds, weevil damage, etc. pendulantfruit Finger pendulance quality Overall fruit pendulance quality The overall estimation fruit pendulance on a bunch Ratio of belowground (root) to aboveground (shoot) dry mass Ratio - computation dateplant The date of planting the plant. Date of planting Date of planting Tallest sucker number of functional leaves - counting Count how many functional leaves (leaves that have 50% or more of their surface as green, healthy, photosynthetic tissue) are on the tallest sucker. Index of black leaf streak disease severity Percentage of plants with changes in new leaves caused by Fusarium wilt Combined percentage with changes in new leaves Percentage of plants with changes in new leaves, caused by Fusarium wilt. Fruit smashed pulp aroma/smell The pulp aroma of the smashed pulp of a mature fruit Aromas The dry weight of the whole plant. Plant dry weight plantDryMass Dry weight Meredith and Lawrence (1969) - complete Index of non-spotted leaves Matooke sweetness basic taste produced by dilute aqueous solutions of natural or artificial substances such as sucrose MatSweet Matooke Sweetness moisture scale 0-10 Dwarfism The presence or absence of dwarfism on a mat Dwarfism of plants dwarfism Species present The nematode species found in the banana plant root sample. Nematode species Weighing of water loss though system normalized by leaf area Water loss - measurement Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked lowest in preference ranking exercise Focus group discussion for lowest ranked traits with male respondents Main underlying colour of pseudostem Main colour of the pseudostem under the outermost sheath. Main underlying colour Average head capsule width Banana weevil larvae average head capsule width The average width of a banana weevil larvae head capsule. Number The number of all leaves (including dried/dead/wilted/yellow) in a shoot system Leaf number numLeaves BD colour chart A - 2016 Calculate as: the Rank of previously marked leaf at one point in time, minus 1 (equivalent to the Rank of marked youngest leaf), divided by the time elapsed between the two Date of data collection events when the marked leaf was recorded as 1) the Rank of marked youngest leaf and 2) the Rank of previously marked leaf, e.g. (2 - 1) / 1 week = LER of 1 leaves/week during a particular time period. Weekly leaf emission rate - computation Adult weevil body - measurement Electronic weighing method The length of a pseudostem Pseudostem length Length lengthPseudostem Number of Pratylenchus coffeae per soil sample Population density Number of Pratylenchus coffeae in soil Visually observe and record the amount of the surface area of a standing leaf that is affected by black leaf streak disease. Black leaf streak disease severity - estimation blotch color scale (1 to 4) Ratio of pseudostem dry mass versus plant dry mass Ratio - computation Percentage damage to upper cross-section inner corm caused by weevils Damage percent X-upper-inner The amount of damage to the inner corm (central cylinder) upper cross-section, assessed by the percentage of tunnels caused by weevils. Calculate as: the sum of the Finger external length measurements, divided by the number of those measurements, e.g. (30 + 34 + 32 + 33 + 33 + 31) / 6 = 32.1. Average finger external length - computation BD colour chart B - 1996 Number of nematodes (Radopholus similis ) per unit fresh root weight - method Count from 100 ml of soil sample t/unit of area/y yellowness scale 0-10 Population density Number of Pratylenchus goodeyi per fresh root weight Number of Pratylenchus goodeyi in roots A root weight trait which is the fresh weight of a root rootFreshMass Root fresh weight Fresh weight Angle to the vertical Bunch position Angle between the axis of the bunch and the vertical Dry weight - measurement 80?C, for 14 days, or longer untill dry Count only fully developed fruit. If there is an even number of hands, there will be two middle hands. Count the middle hand that developed first. Number of fruits on mid-hand of the bunch - counting Measurement under microscope Weevil larvae body length - measurement pestdiseasesampledescription Pest/disease assessment/sample collection description A description of the pest and/or disease assessment and/or sample collection being done at the time of data collection. Pest and/or disease assessment and/or sample collection description Fruit unsmashed pulp taste Taste The taste of the unsmashed pulp of a mature fruit Colour Main colour of the petiole margin. Where the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Petiole margin colour The circumference of the pseudostem of the plant at 20 cm from the collar. plantcircumf20cm Plant circumference 20 cm from the collar Circumference 20 cm above the ground Date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1 The date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1. Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked third lowest in preference ranking exercise Focus group discussion for third lowest ranked traits with male respondents Plant crop cycle - computation Calculate as: the time elapsed between the Date of planting to the Date of harvest. E.g. 24/12/2014 - 24/01/2014 = 334 days. peeper sucker Volume System water loss waterLossSyst The amount of water loss in the system over time Bunch shape Shape of the fully developed bunch on a plant Shape Number of leaves with a black leaf streak disease severity grade of 5 The number of leaves on a plant with a black leaf streak disease severity grade of 5. Number of leaves with black leaf streak disease severity grade 5 Number of Hoplolaimus spp. per soil sample Number of Hoplolaimus spp. in soil Population density Colour of the internal face Colour of bract internal face Main colour of the first external unlifted bract internal face Leaf area - measurement Top view image of loose leaves Dry mass accumulation over time Growth growthDry Dry plant growth Dry weight - measurement 80?C, for 14 days, or longer untill dry Preference scale Harvest density - computation Calculate as: the Plant density multiplied by the Harvest proportion, e.g. 1,667 * 0.75 = 1,250. Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 - estimation Record the date of event. Nematode root knot galling presence - estimation Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. From the functional roots, randomly select five primary roots that are at least 10 cm, and reduce their length to 10 cm. Determine the presence/absence of root knot nematodes, observed externally as galls on the roots. Detach the outermost sheath from the pseudostem (the sheath should not be too dry). Record the overall impression of colour of the exposed surface of the underlying pseudostem. Note that this main colour should cover more than 75% of the underlying pseudostem surface. Use colour chart A and observe out of direct sunlight. Pseudostem colour - estimation plants not harvested/plants planted Calculate as: multiply the distance of the spacing between the plants, both latitudinally and longitudinally, e.g. 2 m x 3 m = 6 m2. Plant spacing - computation Record the rank (order) of the second-most distal hand in the bunch, starting with the hand at the proximal end (closest to the pseudostem) of the bunch as 1 and continuing to the hand at the most distal end (closest to the male bud). Hand rank - counting Circumference at collar plantcircumfcollar The circumference of the pseudostem of the plant at the collar. Plant circumference at collar Overall bunch quality goodbunch Quality The overall estimation of the bunch quality on a mat Finger internal length Internal length The length of a finger measured along the internal (ventral) arc, excluding the pedicel and the fruit tip. fingerintlength crop cycle duration/year avepulpdiam The average lateral (side to side) diameter of the pulp of a fruit. Average lateral diameter of the pulp Average fruit pulp lateral diameter Color of the surface of the sample from light yellow to bright yellow Matooke Matooke surface yellowness intensity MatSurfYellV Surface yellowness intensity Moisture in mouth Matooke perception of moisture content of a food by the tactile receptors in the mouth and also in relation to the lubricating properties of the product Matooke moisture MatMoistM Number of adult larvae - counting Visual counting method Water content - computation (Pseudostem Fresh Weight - Pseudostem Dry Weight) / Pseudostem Fresh Weight yyyy/mm/dd rachis type scale (1 to 2) Rank hand rank Hand rank The rank (order) of a hand in the bunch. handid Body weight The weight of a banana weevil larvae body. Banana weevil larvae body weight Calculate as: the sum of the Number of fingers in hand, divided by the Number of hands in bunch, e.g. (10 + 10 + 11 + 10 + 10 + 10 + 12 + 12 + 12 + 11 +12 + 12 + 10 + 10 + 10 + 11) / 16 = 10.8. Average number of fingers in hand - hands - computation The number of functional leaves (greenness>50%) in a shoot system numFuncLeaves Number Functional leaf number The percentage of all roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant that are functional. Percentage of functional roots Percentage of functional roots Moistness scale 0-10 Selection traits (text) Weighing of water loss though system between timepoints (refer to experimental metadata) Water loss - measurement Volume waterLossPlant The amount of water loss through the plant over time Plant water loss Fruit apex point - estimation Visually observe the fruit apex point and choose the option that is most similar, from the 5 photos of descriptor 6.7.6 in the reference material. moldability scale 0-10 Mealiness scale 0-10 Count the number of leaves (functional only > 50% estimated green area) Leaf number - counting The date of the appearance on the plant of any of the external disease symptoms - yellowing leaves, splitting pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Date of appearance of external symptoms caused by Fusairum wilt Date of first external disease symptoms caused by Fusarium wilt The texture of the smashed pulp of a mature fruit Texture Fruit smashed pulp texture The color of the unsmashed pulp of a mature fruit Fruit unsmashed pulp colour Colour Male bud shape - computation Calculate the ratio w/y, i.e. the broadest width of the male bud to the total length of the male bud. Do not measure the dimensions along the bud but rather on a projection/outline of the bud (e.g. trace the outline of the bud on paper) and use ruler or measuring tape. Number of nematodes per unit of fresh weight The number of nematode individuals per unit of fresh root weight. Root nematode density deathdens Combined density of plants that are dead The number of plants that are dead, in a defined area. Death density Root nematode density - computation Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant, do not include roots from adjacent plants. Carefully wash the soil from the roots with tap water. Cut the roots into 10 cm long pieces and dry with paper tissue. Take a subsample of 15 g, add 100 ml distilled water and store the roots at 4 degrees Celsius. Put the roots in 100 ml of distilled water in a kitchen blender. Macerate the roots 3 times for 10 secs (separated by 5 sec intervals). Pour the macerated suspension through 250, 106 and 40 micro-metre sieves and rinse the sieves with tap water. Using distilled water, collect in a beaker the nematodes from the 40 micro-metre sieve. Using distilled water, dilute to 200 ml the nematode suspension in a graduated cylinder. Blow air through the nematode suspension with a pipette (to homogenise the suspension). Take a subsample of 6 ml (counting dish) or 2 ml (counting slide). Count the nematodes in the counting dish (stereo microscope) or in the counting slide (light microscope). Calculate the final nematode population per unit of fresh root weight. Peripheral damage - measurement Visual observation The date a youngest leaf spotted is first observed on the plant, caused by black leaf streak disease. Date of first youngest leaf spotted Date of first youngest leaf spotted MatAroPumpA Aroma of pumpkin. Possibly, precise if the taste is like boiled pumpkin or fried... Matooke Pumpkin-like aroma Matooke aroma pumpkin Weevil larvae body weight - measurement Measurement under microscope Time from flowering to harvest Time from flowering to harvest The time elapsed from flowering (when the last bracts fall from the most distal hand to display the female flowers) to the harvest of the bunch. flower2harvest MatAstr Matooke complex sensation, accompanied by shrinking, drawing or puckering of the skin or mucosal surface in the mouth, produced by substances such as kaki tannins or sloe tannins Astringency Matooke astringency Number of leaves with black leaf streak disease severity grade 2 Number of leaves with a black leaf streak disease severity grade of 2 The number of leaves on a plant with a black leaf streak disease severity grade of 2. Date of recognising off-type - estimation Record the date of event. rootToPlantMassRatio_dry Measure for the biomass allocation: dry mass of roots versus whole plant dry mass ratio Ratio Root to whole plant mass ratio Individual female respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. Preference scoring scale female respondents The number of plants that are planted. Number of plants planted numplanted Combined number of plants planted The estimated number of fingers in the bunch based on a calculation using the number of fingers in the third and second-most distal hands and the number of hands in the bunch. estnumfingers Number of fingers Calculated number of fingers in bunch The date the plant is first recognised as an off-type. Date of recognising off-type Date of recognising off-type dateofftype finger Measure from the scar on the rachis until the beginning of the fruit. Record on the inner fruit in the middle of the mid-hand of the bunch. Tip: use string to measure or trace outline of fruit on paper. Record the exact value. Fruit pedicel length - exact value - measurement Number of Pratylenchus spp. in soil Population density Number of Pratylenchus spp. per soil sample Visual assessment. Rating of internal symptoms of Fusarium wilt in the greenhouse - estimation Male bud shoulder - computation Calculate the ratio w/y, i.e. the broadest width of the male bud to the total length of the male bud. Do not measure the dimensions along the bud but rather on a projection/outline of the bud (e.g. trace the outline of the bud on paper) and use ruler or measuring tape Average finger circumference - computation Calculate as: the sum of the Finger circumference measurements, divided by the number of those measurements, e.g. (7.0 + 8.0 + 9.0 + 10.0 + 7.0 + 9.5) / 6 = 8.4. 2 pt scale Smoothness in mouth Matooke smoothness Matooke geometrical textural attribute relating to lack of presence of particles in a product MatSmoothM Presence of splitting pseudostem base caused by Fusarium wilt - estimation Visual assessment. Number of nematodes (Pratylenchus spp.) per unit fresh root weight - method Count from 100 ml of soil sample Plant state description - estimation Record a description of the state of the plant. Percentage of plants with a splitting pseudostem base caused by Fusarium wilt Percentage of plants with a splitting pseudostem base, caused by Fusarium wilt. Combined percentage with splitting pseudostem base Presence of yellowing leaves caused by Fusarium wilt - estimation Visual assessment. Number of leaves with black leaf streak disease severity grade 0 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 0. Measure the length of the internal arc of a fruit, without pedicel. Record on the inner fruit in the middle of the mid-hand of the bunch. If there is an even number of hands, there will be two middle hands so use the upper hand that developed first. Record the range. Fruit length - range - measurement Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked second highest in preference ranking exercise Focus group discussion for second ranked traits with male respondents No. per 10g Winged petiole margin - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards. [x Observe at flowering time.] Number of fingers in 2nd hand The number of fingers in the second hand. numfingers2ndhand Number of fingers mm Calculate as: the sum of the number of values for Presence of petiole collapse caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 84 / 300 * 100 = 28%. Percentage of plants with petiole collapse caused by Fusarium wilt - computation Visual appearance - estimation Subjective visual assessment of smashed and unsmashed fruit pulp The number of fingers used to obtain a measurement. Number used to obtain a measurement Number of fingers measured numfingersmeas The number of all types of suckers in the mat. numsuckers Number of suckers Number of all types of suckers fruit relicts scale (1 to 3) Number of nematodes (Meloidogyne spp.) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Pratylenchus goodeyi) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Cut multiple hands from the rachis and weigh them together, using scales. Associate the data with the Hank rank and the Number of hands measured. Multiple hand weight - measurement The number of hands used to obtain a measurement. Number of hands measured Number used to obtain a measurement numhandsmeas Number of fingers numfingershand Number of fingers in hand The number of fingers in a hand. bract imbrication scale (1 to 3) Individual male respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. Preference scoring scale male respondents The relative water content in a root Root relative water content rwcRoot Content Calculate as: the Number of dead roots divided by the Number of all roots, multiplied by 100, e.g. 8 / 20 * 100 = 40 % dead roots. Percentage of dead roots - computation Calculation from count Nematode multiplication from single root inoculation - computation For the plant, the complete corm should be removed from the soil, the roots cut off and excess soil removed. Cross-sections of the corm should be cut (using a guillotine or other suitable device) to obtain five slices of equal thickness. The upper surface of each cut section should be examined and visually evaluate the extent of the vascular discolouration. Distance of internal discolouration in pseudostem of plant caused by Fusarium wilt Matooke Mechanical textural attribute relating to the force required to achieve a given deformation, penetration, or breakage of a product. MatFirmM Matooke firmness Firmness in mouth Black leaf streak disease incubation time Time elapsed from Bruns stage B of unrolling to Foures black leaf streak disease severity scale 1 DIT The time elapsed between a tagged cigar leaf at Bruns stage B of unrolling and the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1. The third leaf (Leaf III) is counted from the last leaf produced before [x bunch emergence] [shooting]. Cut the petiole halfway between the pseudostem and the leaf blade and examine the cross section. [x Observe at flowering time]. Margin behaviour on petiole canal of third leaf - estimation The number of plants that are harvested, in a defined area. Combined density of plants harvested Harvest density harvdens fusion of pedicel scale (1 to 2) Date of harvest Date of harvest The date the bunch is harvested from the plant. dateharvest kg Main colour of the backside of the compound tepal Main colour Compound tepal main colour Number of nematodes (Helicotylenchus multicinctus) per unit fresh root weight - method Count from 100 ml of soil sample petiole-margin contrast Male rachis type Behaviour of male rachis - truncated or present. Truncated means there is no bract scar below the last hand. Present means there is a degenerated or persistent male bud Presence The length of a leaf totLeafLength Total length of all leaves Length numharv Combined number of plants harvested Number of plants harvested The number of plants that are harvested. Total finger weight The weight of all the hands of the bunch, excluding the rachis. totalfingerweight Combined weight The proportion of the crop cycle that takes place in one year. anncropcycprop Proportion of crop cycle that takes place in one year Annual crop cycle proportion Cause of death The cause of death of the plant. Cause of death causedeath Sourness Matooke gustatory complex sensation, generally due to presence of organic acids MatSour Matooke sourness BD colour chart B - 2016 Nematode lifecycle stage Lifecycle stages present The lifecycle stage(s) of the nematode species found in the banana plant root sample. Growth - computation Pseudostem height difference between timepoints (refer to experimental metadata) per number of new leaves formed between timepoints (refer to experimental metadata) clasping or not clasping The number of plants that are planted, in a defined area. Combined density of plants planted Plant density plantdens Put a part of the sample in the mouth and evaluate the intensity of astringency impression due to the sample Astringency method Calculate as: the time elapsed between the Date of planting or the Date of harvest of the bunch from the plant of the previous crop cycle and the Date of first external disease symptoms caused by Fusarium wilt. Time from start of crop cycle to first external disease symptoms caused by Fusarium wilt - computation Formation Stunting expressed as number of leaves per height growth leavesFormedPerPseudostemGrowth Leaves formed per pseudostem height increase Bunch weight - measurement Harvest the bunch by cutting the peduncle 10 cm above the first ridge above the most proximal, hand and immediately below the most distal hand and weigh the bunch (including the rachis), using scales. Multiple finger weight multfingerweight Weight The weight of multiple fingers. Finger external length External length fingerextlength The length of a finger measured along the external (dorsal) arc, excluding the pedicel and the fruit tip. plantheightneck The height of the pseudostem of the plant from the collar to the curved neck of the peduncle. Plant height to neck of peduncle Height smoothness scale 0-10 Calculate as: the Plant density multiplied by the Death proportion, e.g. 1,667 * 0.17 = 283. Death density - computation fruit pedicel scale (1 to 3) fruit relicts scale (1 to 4) margin behaviour scale (1 to 5) Number of Radopholus similis per soil sample Population density Number of Radopholus similis in soil Petiole margins winged Wing presence Behaviour of the petiole margins - winged or not winged. the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Calculate as: Bunch weight minus the sum of the Hand weight measurements coming from the count of number of hands (equivalent to Number of hands in bunch), e.g. 50 - (2.4 + 3.6 + 3.2 + 4.1 + 4.0 + 3.9 + 3.6 + 3.3 + 3.3 + 2.9 + 2.9 + 2.8) = 10.0. Rachis weight - computation Count how many fingers are in the bunch. Number of fingers in bunch - counting Number of Meloidogyne spp. in roots Number of Meloidogyne spp. per fresh root weight Population density DET The time elapsed between the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1, to 6. Time elapsed from Foures black leaf streak disease severity scale 1 to 6 Black leaf streak disease evolution time Pseudostem to whole plant mass ratio Ratio pseudostemToPlantMassRatio_dry Measure for the biomass allocation: dry mass of pseudostem versus whole plant dry mass ratio The overall estimation of fruit parthenocarpy level on a bunch goodfruitfill Parthenocarpy Parthenocarpy Number of Pratylenchus coffeae in roots Population density Number of Pratylenchus coffeae per fresh root weight Fusion of pedicels Fusion Degree of fusion of the pedicels before they join the rachis bad good Death proportion - computation Calculate as: the Number of plants dead, divided by the Number of plants planted, e.g. 4 / 24 = 0.17. Cause of death - estimation Record the apparent cause of death of the plant, e.g. strong winds, Fusarium wilt, etc. fruitpulpcolour Pulp colour Fruit pulp colour The colour of the fruit pulp. sword sucker numswordsuckers The number of sword suckers (suckers with narrow leaves and a large rhizome) in the mat. Number of sword suckers Number of sword suckers Put a part of the sample in the mouth and evaluate the intensity of the sourness Sourness method Count the number of leaves Leaf number - counting Genotype selection traits Selection criteria for genotype ranked highest by respondents Description of traits considered important in selection of best genotype by respondents Take a part of the sample between fingers and evaluate how hard the sample is Hardness in hand method The area allocated to one plant. Area allocated to one plant Plant spacing plantspacing Percentage of dead roots The percentage of all roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant that are dead. Percentage of dead roots Behaviour of the petiole margins - winged or not winged. the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Margins clasping or not clasping Petiole margins clasping Number of plants planted - computation Calculate as: the sum of the number of values for Date of planting. leaf rank sucker Water content - computation (Root Fresh Weight - Root Dry Weight) / Root Fresh Weight Moistness Mouth impression method Once the flavors are detected, moisture is felt by turning the product in the mouth Lobe colour of tip of compound tepal Main colour of the tip of the compound tepal (lobe) Lobe colour at tip of the tepal The percentage of damage to the periphery of the peeled corm, assessed by tunnels caused by weevils Peripheral damage caused by weevils Damage to periphery of peeled corm caused by weevils Presence of root knot nematodes Nematode root knot galling presence The presence of root knot nematodes, observed externally as galls on the roots. Black leaf streak disease severity index - computation Calculate as: ((((Number of leaves with black leaf streak disease severity grade 0, multiplied by 0), plus (Number of leaves with black leaf streak disease severity grade 1, multiplied by 1), plus (Number of leaves with black leaf streak disease severity grade 2, multiplied by 2), plus (Number of leaves with black leaf streak disease severity grade 3, multiplied by 3), plus (Number of leaves with black leaf streak disease severity grade 4, multiplied by 4), plus (Number of leaves with black leaf streak disease severity grade 5, multiplied by 5), plus (Number of leaves with black leaf streak disease severity grade 6, multiplied by 6)), divided by the number of grades in the scale minus 1 (i.e. 7-1)), divided by the Number of standing leaves), multiplied by 100. For example, ((((3 * 0) + (1 * 1) + (1 * 2) + (3 * 3) + (5 * 4) + (0 * 5) + (0 * 6)) / 6) / 13) * 100 = ((32 / 6) / 13) * 100 = 41. Fruit unsmashed pulp smoothness-mouth feel Smoothness-mouth feel The smoothness-mouth feel of the unsmashed pulp of a mature fruit Pigmentation of outer surface Pigmentation of outer surface of cigar leaf on sucker Colour of pigmentation on outer surface of cigar leaf on a developed sucker Calculate as: number of square metres in the area unit of choice, divided by the Plant spacing, e.g. 10,000 square metres in 1 hectare / 6 = 1,667 plants/ha. Plant density - computation The number of fingers in the second-most distal hand Number of fingers in 2nd-most distal hand numfingers2nddistalhand Number of fingers Fruit shape of curve - estimation Visually observe the shape of the curve of the fruit and choose the option that is most similar, from the 5 photos of descriptor 6.7.4. in the reference material. Observe the inner fruit in the middle of the mid-hand of the bunch. In case of an asymmetric bunch that has straight and curved fruits, please indicate it in the note section and score only the fruit on the upper side of the bunch. leaves/week Damage percent X-average The average percentage of damage in the whole corm (the inner corm and the outer corm) caused by weevils. Average percentage damage to the corm caused by weevils Pseudostem height increase over time pseudostemHeightIncrease Growth Height increase of pseudostem sourness scale 0-10 The presence/absence of yellowing leaves, caused by Fusarium wilt. YL Presence/absence of yellowing Presence of yellowing leaves caused by Fusarium wilt Pseudostem height increase per leaf formed Stunting expressed as height growth per new formed leaf Growth pseudostemHeightIncreasePerFormedLeaf Alignment of bracts at the apex of the male bud Imbrication Bract imbrication at apex of male bud Petiole margin colour - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Use colour chart A and observe out of direct sunlight. Record the colour of the margin (general colour is below the rim). [x Observe at flowering time.] Male rachis type - estimation Visually observe the part of the rachis between the last hand and the male bud. Truncated means there is no bract scar below the last hand. Present means there is a degenerated or persistent male bud Number of banana weevil adults per trap How many banana weevil adults caught per trap. Number of banana weevil adults fruit curve shape (scale 1 to 5) Pseudostem relative water content The relative water content in a pseudostem Content rwcPseudostem Finger weight Weight The weight of a finger. fingerweight Population density Number of Pratylenchus spp. in roots Number of Pratylenchus spp. per fresh root weight bud size scale (1 to 3) Genotype selection traits Description of traits considered important in selection of least genotype by respondents Selection criteria for genotype ranked lowest by respondents Rating of external symptoms of Fusarium wilt in the field - estimation Visual assessment. Number of nematodes (Pratylenchus spp.) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Head capsule width The width of a banana weevil adult weevil head. Banana weevil adult head capsule width Surface yellowness intensity Appearance method When you receive the sample, observe the surface and evaluate the intensity of the color Smoothness-Mouth feel - estimation Mouth feel assessment of smashed and unsmashed fruit pulp Preference scoring scale individual respondents Individual respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. The average actual annual yield, taking into account the proportion of plants harvested, from the number of plants planted Average actual annual yield actualannyield Average actual annual yield Growth - computation Leaf area difference between timepoints (refer to experimental metadata) Number of leaves with a black leaf streak disease severity grade of 4 Number of leaves with black leaf streak disease severity grade 4 The number of leaves on a plant with a black leaf streak disease severity grade of 4. topLeafArea Projected top area Area The projected leaf area of a plant (photosynthetic surface) Record the date of event. Date of tagged cigar leaf unrolling at Bruns stage B - estimation Fruit smashed pulp colour Colour The color of the smashed pulp of a mature fruit Plant circumference at collar - measurement Measure the circumference of the pseudostem of the plant at the collar, or from the pseudostem base at the ground if the collar is not visible. datedamage The date damage to the plant is first observed. Date of damage Date of damage Visual assessment. Presence of changes in new leaves caused by Fusarium wilt - estimation Actual annual yield - computation Calculate as: the Bunch weight divided by 1,000, multiplied by the Annual crop cycle proportion, multiplied by the Harvest density, e.g. (50 / 1,000) * 1.09 * 1,250 = 68.13. Fresh weight - measurement Weighing Average weight avehandweight Average hand weight The average weight of the hands. Count how many hands are used to obtain a measurement. Number of hands measured - counting Measure the circumference of a finger at its widest point, using a tape measure. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger circumference - measurement Meredith and Lawrence (1969) - condensed Number of nematodes (Rotylenchulus spp.) per unit fresh root weight - method Count from 100 ml of soil sample g / mL Bract apex shape Shape of the first external unlifted bract of the male bud Apex shape wing presence/absence and clasping presence/absence scale 1 to 5 Male bud shoulder Male bud shoulder description Ratio Calculate as: the sum of Damage to inner corm upper cross-section caused by weevils, Damage to outer corm upper cross-section caused by weevils, Damage to inner corm lower cross-section caused by weevils, and Damage to outer corm lower cross-section caused by weevils, divided by the number of traits included in the sum, e.g. (25 + 36 + 18 + 20) / 4 = 24.8. Average damage to the corm weevils - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 6. Number of leaves with black leaf streak disease severity grade 6 - computation Calculate as: the sum of the Finger internal length measurements, divided by the number of those measurements, e.g. (27 + 31 + 29 + 30 + 30 + 28) / 6 = 29.2. Average finger internal length - computation hand wing presence/absence Egg-laying female root knot nematode Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked highest in preference ranking exercise Focus group discussion for top traits with female respondents Sweetness scale 0-10 Number of nematodes (Pratylenchus goodeyi) per unit fresh root weight - method Count from 100 ml of soil sample color homogeneity scale 0-10 BoiPlantnStic Force required to peel off the fraction of product adhering to the interior of the oral cavity Boiled plantain Stickiness Boiled plantain Stickiness Number of leaves with black leaf streak disease severity grade 2 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 2. Matooke Surface color homogeneity Uniformity of color of the surface of the sample Matooke homogeneity of surface colour MatSurfHomColV Cut the hands off the rachis and weigh the rachis, using scales. Rachis weight - measurement Count how many water suckers are in the mat. Number of water suckers - counting Thickness of the peel Finger peel thickness peelthickness The thickness of the peel of a finger. Count how many fingers are in the third hand. Number of fingers in 2nd hand - counting numpeepersuckers The number of peeper suckers (suckers less than 15 cm tall) in the mat. Number of peeper suckers Number of peeper suckers Chewiness µM The time elapsed from the start of the crop cycle to the appearance on the plant of any of the external disease symptoms - - yellowing leaves, splitting pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Time from start of crop cycle to first external disease symptoms caused by Fusarium wilt Time from start of crop cycle to appearance of external disease symptoms caused by Fusarium wilt Calculate as: the time elapsed between the Date of harvest of the bunch of the plant from the preceeding crop cycle and the Date of harvest of the bunch of the plant from the current crop cycle, e.g. 24/11/2015 - 24/12/2014 = 335 days. Ratoon crop cycle - computation Rating of external symptoms of Fusarium wilt in the field Leaf yellowing and wilting caused by Fusarium wilt rating A rating of the extent of leaf yellowing and wilting, caused by Fusarium wilt. Visual estimation of dwarfism, for Early Evaluation Trial stage only. Dwarfism - estimation A description of what made the plant recogniseable as an off-type. describeofftype Off-type description Description of how off-type Elemental flavor caused by dilute aqueous solutions of various substances such as sucrose or aspartame BoiPlantnSwt Boiled plantain Sweetness Sweetness Boiled plantain parthenocarpy Number of fingers Number of fruits on the mid-hand of the bunch **subsume Number of fruits on mid-hand of the bunch The date of data collection. datedatacoll Date of data collection Date of data collection Measure the distance from the collar, or from the pseudostem base at the ground if the collar is not visible, to the intersection of the petioles of the two youngest leaves (leaf ranks 1 and 2), using a measuring pole or sliding ruler. Plant height - petiole of two youngest leaves - measurement nematode lifecycle stages Extent of discolouration The extent of discolouration in the corm of the tallest sucker, caused by Fusarium wilt. Extent of internal discolouration in corm of tallest sucker caused by Fusarium wilt Transpiration efficiency - computation Gram accumulated biomass per volume water transpired LER Weekly leaf emission rate A rate that expresses the number of leaves that have emerged from the pseudostem in a week. Weekly leaf emission rate Percentage of plants with internal disease symptoms caused by Fusarium wilt The percentage of plants with any or multiple of the internal disease symptoms - discolouration in the corm, and/or discolouration in the pseudostem, caused by Fusarium wilt. Combined percentage with internal symptoms of Fusarium wilt Visual counting method Number of weevil larvae - counting Number of Helicotylenchus multicinctus per soil sample Population density Number of Helicotylenchus multicinctus in soil The time elapsed from planting to the death of the plant. plant2death Time from planting to death Time from planting to death Calculate as: the Number of dead roots plus the Number of functional roots, e.g. 8 + 12 = 20 roots. Number of all roots - computation Record a description of what made the plant recogniseable as an off-type. Off-type description - estimation Behaviour before falling, whether revolute or not revolute Bract behaviour before falling Behaviour of the last lifted bract - revolute or not revolute Anther colour - estimation The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the anther colour on the face opposite to the dehiscence split of the anther. Use colour chart B and observe out of direct sunlight. Fusarium wilt external symptoms scale BD colour chart A - 1996 m2 Distance of internal discolouration in pseudostem of tallest sucker, caused by Fusarium wilt Distance of internal discolouration in pseudostem of tallest sucker caused by Fusarium wilt For the tallest sucker, the complete corm should be removed from the soil, the roots cut off and excess soil removed. Cross-sections of the corm should be cut (using a guillotine or other suitable device) to obtain five slices of equal thickness. The upper surface of each cut section should be examined and visually evaluate the extent of the vascular discolouration. Colour of the external face Colour of bract external face Main colour of the first external unlifted bract external face variable_of BoiPlantnMeal Mealiness Boiled plantain Boiled plantain Mealiness Mechanical property linked to cohesion and the presence of fine particles in the product during chewing Calculate as: the sum of the Number of fingers in 3rd hand and the Number of fingers in 2nd-most distal hand, divided by 2, and multiplied by the Number of hands in bunch, e.g. (16 + 12) / 2 * 8 = 112. Number of fingers in bunch - estimation Calculate as: the Bunch weight multiplied by the Annual crop cycle proportion, e.g. 48 * 1.09 = 52.32. Annual plant productivity - computation Date of data collection - estimation Record the date of event. Nematode multiplication from single root inoculation of 50 nematodes per 8 cm length after 12 weeks Nematode reproduction factor (microplot screening) Population density Recorded from the base of the pseudostem to the emerging point of the peduncle. Pseudostem height - estimation Male bud length - measurement Measure the length (y) of a projection/outline of the male bud on paper, with a measuring tape or ruler. plants harvested/plants planted Circumference 1 m above the ground Plant circumference 100 cm from the collar plantcircumf100cm The circumference of the pseudostem of the plant at 100 cm from the collar. Ex: g/100 mL juice???? Fruit smashed pulp taste Taste The taste of the smashed pulp of a mature fruit Percentage of plants with yellowing leaves caused by Fusarium wilt - computation Calculate as: the sum of the number of values for Presence of yellowing leaves caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 78 / 300 * 100 = 26%. Lateral diameter of the pulp pulpdiam The lateral (side to side) diameter of the pulp of a fruit. Fruit pulp lateral diameter Blotches relative area at petiole base Percentage coverage of petiole base by blotches. Blotch surface area Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 and the Date of tagged cigar leaf unrolling at stage B, e.g. 24/04/2014 - 24/03/2014 = 31 days. Black leaf streak disease incubation time - computation finger Count how many peeper suckers are in the mat. Number of peeper suckers - counting Number Number of dead roots The number of dead roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Presence absence 1 The relative water content in a leaf rwcLeaf Content Leaf relative water content Harvest proportion - computation Calculate as: the Number of plants harvested, divided by the Number of plants planted, e.g. 18 / 24 = 0.75. Calculate as: the sum of the number of values for Date of harvest. Number of plants harvested - computation weevil Number of adult weevil trap - counting Visual counting method of Weevil traps (the trap should be minimum 30 cm long). Time from planting to shooting - computation Calculate as: the time elapsed between the Date of planting and the Date of shooting. E.g. 24/09/2014 - 24/01/2014 = 243 days. Put a part of the sample in the mouth, chew and evaluate the quantity of water within the sample. Moisture in mouth method Matooke hardness mechanical textural attribute relating to the force required to achieve a given deformation, penetration, or breakage of a product. MatHardT Matooke Hardness in hand height score (1 to 3) Presence absence 0 The number of leaves on the tallest sucker that have 50 % or more of the leaf surface area as green, healthy, photosynthetic tissue. tallsuckererfunctleaves Tallest sucker number of functional leaves Number of functional leaves Firmness Sensory Texture method Put in the mouth a piece of banana and Evaluate the force necessary to obtain the deformation of the product between the teeth during the first compression Damage description - estimation Record a description of the damage to the plant, e.g. leaves ripped, pseudostem snapped, plant toppled, etc. Youngest leaf spotted Rank of youngest leaf spotted The rank (order) of the first fully unfurled leaf with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 leaf spot light-coloued dry centres, caused by black leaf streak disease. YLS Number of water suckers numwatersuckers The number of water suckers (suckers with broad leaves, a small rhizome, and a weak connection to the plant) in the mat. Number of water suckers Shape of male bud Shape Male bud shape fingercircumf Finger circumference The circumference of a finger. Circumference scale_of A rating of the extent of rhizome (corm) discolouration, caused by Fusarium wilt. Rating of internal symptoms of Fusarium wilt in the greenhouse Discolouration caused by Fusarium wilt rating Total finger weight - measurement Cut the hands off from the rachis and weigh all the hands together, using scales. Calculate as: the sum of values with a Black leaf streak disease severity grade of 3. Number of leaves with black leaf streak disease severity grade 3 - computation When you receive the sample, observe the surface and evaluate the homogeneity Surface color homogeneity Appearance method Number of hands on whole bunch - counting On a bunch with mostly hands of > 10 fingers, a possible ultimate hand with 1-5 (rather smaller) fingers should not be counted. bunch position scale(1 to 5) Record the date of event. Date of first youngest leaf spotted - estimation Male bud size - measurement Length and maximum diamter of male bud at harvest. Average fruit pulp lateral diameter - computation Calculate as: the sum of the Fruit pulp lateral diameter measurements, divided by the number of those measurements, e.g. (3.1 + 4.8 + 3.7 + 4.6 + 5.6 + 6.4) / 6 = 4.7. Time from planting to first youngest leaf spotted Time elapsed from planting to first youngest leaf spotted Time elapsed between planting and the appearance of the first youngest leaf spotted. Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. From the functional roots, randomly select five primary roots that are at least 10 cm, and reduce their length to 10 cm. Where root knot nematode galls are present, determine the extent of the galling using the scale. Nematode root knot galling extent - estimation CNL Presence/absence of changes The presence/absence of changes in new leaves - irregular pale margins, narrowing of lamina, burning plus ripping of lamina, lamina becoming more erect, caused by Fusarium wilt. Presence of changes in new leaves caused by Fusarium wilt Blotches colour - estimation Observe visually the colour of the blotches on the upper leaf sheath. Finger lateral diameter The lateral (side to side) diameter of a finger. Lateral diameter fingerlatdiam Presence of petiole collapse caused by Fusarium wilt - estimation Visual assessment. Press a piece of banana between the molars and appreciate the adhesion of the product Stickiness Sensory Texture method Water content - computation (Plant Fresh Weight - Plant Dry Weight) / Plant Fresh Weight Cut a finger in half, crosswise at its widest point, and on the cross-section measure the width of the peel, using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger peel thickness - measurement Calculate as: the sum of the Number of fingers in hand measurements from all the hands in the bunch (number equivalent to Number of hands in bunch), e.g. 16 + 17 + 19 + 18 + 19 + 18 + 17 + 17 + 16 + 14 + 14 + 13 = 198. Number of fingers in bunch - computation Presence of splitting pseudostem base caused by Fusarium wilt Presence/absence of splitting SP The presence/absence of a splitting pseudostem base, caused by Fusarium wilt. BD colour chart A - 2016 Number of Hoplolaimus spp. per fresh root weight Population density Number of Hoplolaimus spp. in roots Weighing of water loss though plant between timepoints (refer to experimental metadata) Water loss - measurement Presence of changes in new leaves caused by Fusarium wilt - estimation Visual assessment. Record the date of event. Date of harvest - estimation leaf The amount of water loss in the system normalized by the leaf area System water loss per leaf area Volume waterLossSystPerLeafArea Bract apex shape - estimation Refers to the first external unlifted bract. Visually observe the apex shape of flattened bracts to determine shape and choose the option that is most similar, from the 4 photos of descriptor 6.5.2. in the reference material. bunch maturity Visually observe the colour of the internal face of the first unlifted bract. Use colour chart A and observe out of direct sunlight. Colour of bract internal face - estimation Cut a transverse cross section of the corm at 10 cm below the collar (lower cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Damage to lower outer corm weevils - measurement Mealiness Sensory Texture method Put a piece of banana in your mouth and assess the presence of mealiness particles during chewing Number of functional roots - counting Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. Count the number of functional roots. method_of BD colour chart A - 1996 Male rachis appearance - estimation Visually observe the male rachis appearance and choose the option that is most similar. Calculate as: the sum of the Finger peel thickness measurements, divided by the number of those measurements, e.g. (2 + 2 + 2 + 3 + 2 + 3 + 3) / 6 = 2. Average finger peel thickness - computation Population density Number of Meloidogyne spp. in soil Number of Meloidogyne spp. per soil sample Bunch weight Weight The weight of the bunch, including the rachis. bunchweight The time elapsed from planting to shooting (when the inflorescence emerges from the pseudostem and is still in an erect position). plant2shoot Time from planting to shooting Time from planting to shooting Margin behaviour on petiole canal of third leaf Margin behaviour of the petiole canal of the third leaf before bunch. Behaviour fruit length scale (1 to 5) Percentage of plants with yellowing leaves, caused by Fusarium wilt. Percentage of plants with yellowing leaves caused by Fusarium wilt Combined percentage with yellowing leaves Average finger lateral diameter - computation Calculate as: the sum of the Finger lateral diameter measurements, divided by the number of those measurements, e.g. (3.5 + 5.2 + 4.1 + 5 + 6 + 6.8) / 6 = 5.1. The average weight of a banana weevil larvae body. Average body weight Banana weevil larvae average body weight Measure the length of a finger that is at the ready to eat stage, along the external (dorsal) arc, excluding the pedicel and the fruit tip, using a tape measure. Associate the data with the Hand rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger external length - measurement Open question The rank (order) of the oldest (most-distal) standing leaf (with an erect petiole). Rank of oldest standing leaf Rank of oldest standing leaf The extent to which vascular discolouration extends up the pseudostem of the tallest sucker should be determined by making cross-section cuts, from the base of the pseudostem upwards, and examining the internal tissues following each cut. The point at which discolouration is no longer visible should be noted and the distance from this point to the pseudostem base recorded. Extent of internal discolouration in corm of tallest sucker caused by Fusarium wilt - estimation Appearance of flowers/bracts on the male rachis Male rachis appearance Appearance in relation to the presence or absence of neutral, male and/or hermaphrodite flowers Average internal length The average length of the fingers measured along the internal (ventral) arc, excluding the pedicel and the fruit tip. avefingerintlength Average finger internal length Focus group discussion for third ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked third highest in preference ranking exercise astringency scale 0-10 Growth - computation Pseudostem height difference between timepoints (refer to experimental metadata) cm? Preference score individual respondents Researchers calculate the preference score by respondents aroma scale 0-10 plantFreshMass Fresh weight Plant fresh weight A plant weight trait which is the fresh weight of a plant PA Assessment by respondents of physical features of a plant by giving it a score on the preference scale Overall plant appearance Overall plant appearance Black leaf streak disease severity An indication of the extent of damage on an individual leaf caused by black leaf streak disease. Black leaf streak disease severity BLS roots Identify the nematode stage, using the options. Nematode stage - estimation Date of tagged cigar leaf unrolling at Bruns stage B Date of tagging at Bruns stage B of unrolling The date a tagged cigar leaf is at Bruns stage B of unrolling. The number of leaves on a plant with a black leaf streak disease severity grade of 6. Number of leaves with a black leaf streak disease severity grade of 6 Number of leaves with black leaf streak disease severity grade 6 Sweetness tasting method Put a part of the sample in the mouth and evaluate the intensity of taste of sweetness Visual estimation of fruit pendulance on a bunch, for Early Evaluation Trial stage only. Fruit pendulance - estimation Banana weevil larvae body length Body length The length of a weevil larvae body, from the weevil larvae head to the tip of the abdomen. 5 pt corm discolouration scale Adult weevil head capsule - measurement Measurement under microscope Fresh weight difference between timepoints (refer to experimental metadata) Growth - computation bud length scale (1 to 3) Measure the distance from the collar, or from the pseudostem base at the ground if the collar is not visible, to under the neck of the curved peduncle, using a measuring pole or sliding ruler. Plant height - neck of peduncle - measurement Number of banana weevil larvae measured Number of banana weevil larvae measured How many banana weevil larvae used to obtain a measurement. Lobe colour of tip of compound tepal - estimation The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the colour of the lobe at the tip of the tepal. Use colour chart B and observe out of direct sunlight. Estimate maturity stage of the bunch. Bunch maturity stage - estimation hand Total finger weight - hands - computation Calculate as: the sum of the Hand weight measurements from all the hands in the bunch (number equivalent to Number of hands in bunch), e.g. 2.4 + 3.6 + 3.2 + 4.1 + 4.0 + 3.9 + 3.6 + 3.3 + 3.3 + 2.9 + 2.9 + 2.8 = 40.0. Remains of flower relicts at apex Remains of flower relicts at fruit apex Percentage of flower relicts at fruit apex Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked lowest in preference ranking exercise Focus group discussion for lowest ranked traits with female respondents Leaf length - measurement The longest diagonal of a fitted ellips Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked third lowest in preference ranking exercise Focus group discussion for third lowest ranked traits with female respondents Focus group discussion for third ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked third highest in preference ranking exercise Percentage of plants with petiole collapse caused by Fusarium wilt Percentage of plants with petiole collapse, caused by Fusarium wilt. Combined percentage with petiole collapse Gauhls modification of Stovers severity scoring system (YYYY) Visually observe the pigmentation of the outer surface of the cigar leaf of a developed sucker or on another plant of the same accession. [x Observation should be made before shooting, on a developed sucker or on another plant of the same accession.] Pigmentation of outer surface of cigar leaf - estimation Number of fingers in outer whorl of 2nd-most distal hand - counting Count how many fingers are in the outer whorl of the second-most distal hand. Researchers calculate the preference score by female respondents Preference score female respondents margin behaviour scale (1 to 4) multnumfingshand The number of fingers in multiple hands. Number of fingers Multiple number of fingers in hand bract apex scale (1 to 5) Number of maiden suckers Number of maiden suckers The number of maiden suckers (fully grown suckers with foliage leaves) in the mat. nummaidensuckers Tallest sucker height The height of the pseudostem of the tallest sucker within the same mat as the plant (the sucker that will become the next plant with a bunch, once the bunch from the current plant is harvested) suckerheight Height rachis appearance scale (1 to 5) Visually observe the bract imbrication of the apex of the male bud and choose the option that is most similar, from the 3 photos of descriptor 6.5.3 in the reference material. Bract imbrication at apex of male bud - estimation The longest diagonal of a fitted ellips Leaf length - measurement Average finger circumference Average circumference The average circumference of the fingers. avefingercircumf Preference score male respondents Researchers calculate the preference score by male respondents Leaf fresh weight Fresh weight A leaf weight trait which is the fresh weight of a leaf leafFreshMass The average weight of a banana weevil adult body. Banana weevil adult average body weight Average body weight Fruit pulp colour - estimation Visually observe the fruit pulp colour and select the option in the colour chart that is most representative. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Count how many of all types of suckers are in the mat. Number of suckers - counting Number of spotted leaves - counting Count how many of the standing leaves are spotted (with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres), considering all leaves in between and inclusive of, the youngest leaf and the oldest standing leaf. sweetness scale 0-10 Cut a transverse cross section of the corm at the collar (upper cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Damage to upper outer corm weevils - measurement Petiole margin colour - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Use colour chart A and observe out of direct sunlight. Record the colour of the margin (general colour is below the rim). [x Observe at flowering time.] open answer Top view image Leaf area - measurement Extent of internal discolouration in corm of plant caused by Fusarium wilt - estimation The extent to which vascular discolouration extends up the pseudostem should be determined by making cross-section cuts, from the base of the pseudostem upwards, and examining the internal tissues following each cut. The point at which discolouration is no longer visible should be noted and the distance from this point to the pseudostem base recorded. Vitamin C - measurement Calculate as: the sum of the number of values for Presence of petiole collapse caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 84 / 300 * 100 = 28%. The extent of root knot nematodes, observed externally as galls on the roots. Extent of root knot nematodes Nematode root knot galling extent Plant relative water content Content The relative water content in the whole plant rwcPlant Dry weight - measurement 80?C, for 14 days, or longer untill dry Count from 100 ml of soil sample Number of nematodes (Hoplolaimus spp.) per unit fresh root weight - method Increase of leaf area leafAreaIncrease Leaf area increase over time Growth rachis angle scale (1 to 2) Focus group discussion for second ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked second highest in preference ranking exercise Boiled plantain Moistness BoiPlantnMois Perception of the amount of water absorbed or released by the product Boiled plantain Moistness Calculate as: the sum of the number of plants with any or multiple of the internal disease symptoms caused by Fusarium wilt - discolouration in the corm, and/or discolouration in the pseudostem - divided by the Number of plants planted * 100, e.g. 108 / 300 * 100 = 36%. Percentage of plants with internal disease symptoms caused by Fusarium wilt - computation blotch pigmentation scale (1 to 4) singleLeafLength Single leaf length The length of a leaf Length Distance from the base of the pseudostem to the emerging point of the peduncle. Height Pseudostem height Record the rank of the previously marked leaf (the leaf that was marked in the Rank of marked youngest leaf during the last data collection), counting the leaf rank by starting with the newest completely unrolled leaf as 1 and moving downwards. Associate the data with the Date of data collection. Rank of previously marked leaf - counting fruit curve shape (scale 1 to 6) Mark the youngest completely unrolled leaf and record its rank, which is always 1. Associate the data with the Date of data collection. Rank of marked youngest leaf - counting The number of leaves with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres, caused by black leaf streak disease. Number of spotted leaves Number of spotted leaves harvprop Harvest proportion The proportion of plants that are harvested, from the number of plants planted. Combined proportion of plants harvested Number of nematodes (Hoplolaimus spp.) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Fruit pedical length - range - measurement Measure from the scar on the rachis until the beginning of the fruit. Record on the inner fruit in the middle of the mid-hand of the bunch. Tip: use string to measure or trace outline of fruit on paper. Record the range. Number of standing leaves - counting Count how many of the leaves are standing leaves (without a bent or broken petiole) Chewiness Boiled plantain Chewiness BoiPlantnChew Energy or number of chews necessary to chew the banana to make it ready to be swallowed Boiled plantain Number of leaves with black leaf streak disease severity grade 4 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 4. Main colour Colour of the blotches on the upper leaf sheath of the petiole base. Blotches colour at the petiole base Visual assessment. Presence of yellowing leaves caused by Fusarium wilt - estimation Banana pest disease pulp colour Count from 100 ml of soil sample Number of nematodes (unclassified spp.) per unit fresh root weight - method Fusarium wilt internal symptoms scale The area of a leaf singleLeafArea Leaf area Area hardness scale 0-10 numnotharv Number of plants not harvested The number of plants that are alive but not harvested (e.g. due to slow or poor development). Combined number of plants not harvested fruit apex scale (1 to 5) Visual assessment. Presence of petiole collapse caused by Fusarium wilt - estimation leaves/month Visual estimation of bunch quality, for Early Evaluation Trial stage only. Bunch quality - estimation Nosal smell - estimation Nosal smell assessment of smashed and unsmashed fruit pulp aromas stickiness scale 0-10 Number of standing leaves Number of standing leaves The number of leaves attached to the plant that have an erect petiole (not bent or broken). Time from harvest to death - computation Calculate as: the time elapsed between the Date of harvest of the bunch of the plant from the preceeding crop cycle and the Date of death of the current plant, e.g. 24/03/2015 - 24/12/2014 = 90 days. bud shape scale (1 to 5) Ratio - computation Ratio of leaf dry mass versus plant dry mass Try to make a ball (agglomerate) of the sample and evaluate how easy it is to deform or break the sample Moldability in hand method Select at random five functional primary roots, at least 10 cm long. Reduce the length of the five selected roots to 10 cm and slice through the roots length-wise. Evaluate one half of each of the five roots for the percentage of root cortex showing necrosis. The maximum root necrosis per root half can be 20 %, giving a maximum root necrosis of 100 % for the five halves together. Mentally determine the necrosis of the individual roots (1 to 5) and sum these together to get the total root necrosis of the sample. Root necrosis caused by nematodes - estimation The percentage of plants with any or multiple of the external disease symptoms - yellowing leaves, splitting of pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Percentage of plants with external disease symptoms caused by Fusarium wilt Combined percentage with external symptoms of Fusarium wilt Visual counting method of Weevils per trap (the trap should be minimum 30 cm long) Number of adult weevils - counting Bunch maturity stage at harvest Stage of maturity at harvest The stage of maturity of the bunch at harvest. bunchmaturity Faures stages of development of BLS disease scale (1987) water suckers Smoothness in mouth method Put a part of the sample in mouth, chew it and after 5 chews, evaluate between tongue and palate the number and the size of the particles. The number of leaves on a plant with a black leaf streak disease severity grade of 3. Number of leaves with a black leaf streak disease severity grade of 3 Number of leaves with black leaf streak disease severity grade 3 Fruit shape of curve Shape of a fruit (finger) in the middle of the bunch **subsume Shape of the longitudinal curvature Selection criteria for genotype ranked second lowest by respondents Genotype selection traits Description of traits considered important in selection of second least genotype by respondents No. per 100ml