FRCOL1_FRCOL1M_date USDA FLDIA_FLDIAM_1-9FLDIAUPOVscale UPOV PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale USDA RPO_RPOM_% USDA RosBREED GDR SMYFR_SMYFRM_1-9SUSCEPscale LFRW_FRFRESHWLGM_g USDA Università Politecnica delle Marche SKINCOL_REFLECTCIXM_ChromaIndex Capocasa et al. 2008 SPO_SPOM_1-9SUSCEPscale GDR RosBREED SDE_SDEM_1-9SUSCEPscale RosBREED GDR RosBREED GDR ACHENEVIABLE_ACHVIAM_% Pinkerton & Finn RPP_RPPM_Rf USDA NEM-RF_RPPM_Rf USDA STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale Feldmann, et al. SVD_SVD1-5M_1-5SUSCEPscale UC Davis FRYIELD_FRYIELDTWM_g USDA calyx attachment is sunken below shoulder 5 very firm 9 not reflexed 1 Biswas et al. 2019 University of Delaware LFPUBESC_LFPUBESCM_1-3LFPUBESCscale pinkish white 1 BEARINGTYPE_BEARINGRBM_count RosBREED GDR Università Politecnica delle Marche Capocasa et al. 2008 FRAP_FRAPFSHM_FSHEQumoles/g fruit BLDATE1_FLDATE1M_FLDATE1date USDA USDA RPF_RPF%M_% ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq RosBREED GDR PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale RosBREED GDR RDE_REDM_1-9RESISTscale USDA 2 long conic HD_FIRSTHARVM_date USDA USDA RPOAP_RPOAP%M_% ovate 4 USDA PLANTHT_PLANTHTM_cm Zurn, et al. RMaPh_ RMaPh%M_ % USDA SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale UPOV CALYXSZ_CALYXSZM_1-3CALYXSZscale RosBREED GDR SPOAP_SPOAPM_1-9SUSCEPscale RosBREED GDR stem breaks well above calyx Y** 1 not remontant rhombic 6.0 1 inserted RosBREED GDR FRYIELD_FRYIELD1-9M_1-9FRYIELDscale medium number of flowers 5 USDA RFOF_RFOF%M_% Zurn, et al. TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale UPOV cordate 3 stamens taller than receptacle 9 2 calyx size equal to fruit diameter RosBREED GDR STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale RosBREED GDR SPF_SPFM_0-5SUSCEPscale 9 dark red slightly uneven 2 USDA 5FRW_FRFRESHWT5LM_g highly resistant/no symptoms 9 GDR RosBREED CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale RPCCR_RPCCR%M_% USDA downwards 3 BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale UPOV USDA DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL Pincot et al. 2020 RVD_RVD1-9M_1-9RESISTScale University of California, Davis RosBREED GDR PPMF_PPMFM_0-1PMFscale USDA RTL_RUNTOTLENM_m GDR RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale stem does not break, berry separates from calyx easily N Salinas et al. 2019 University of Florida FRRAFR_FRAFR%M_% USDA FRpH_pH2M_pH level with surface 3 PEDICELLEN_PEDICELLENM_cm USDA FRDEV_FRDEVM_days USDA FRUITSET_FRSETM_1-9FRUITSETscale USDA stamens shorter than receptacle 1 RPCCR_RPCCR1-8M_1-8RESISTscale NCRI Eikemo, et al. SSC_SSCM_SSCg/100g RosBREED GDR RosBREED GDR RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale RosBREED GDR TITA_TITAM_TITAMeq/100gCA USDA FRD_FRRIPEM_days 2 medium density 3 low FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale UPOV Jiménez, et al. UC Davis RPCCR_RPCCR1-5M_1-5RESISTscale pedicle does not break 1 CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale UPOV very sweet, no off-flavors 9 strongly below 1 4 day neutral 3 slightly uneven USDA STEMBREAK_PEDBRKM_text EPILGT_EPILGTM_cm USDA SKINCOL_SKINCOL1-9M_1-9SKINCOLscale RosBREED GDR 1 long conic very light brown or green 9 FRFLCOLDEPTH_FRCOLDEPTHM_% RosBREED GDR tough 9 FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale USDA PLOIDY_FCDAPIICFM_x USDA low yield 3 2 conic FLDATE1_FLDATE1M_FLDATE1date USDA FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale CPBR even 2 DRIPLOSS_DRIPLOSSM_g RosBREED GDR 5.0 medium RMaPh_ RMaPhM_1-5RESISTscale Knapp, et al. UC Davis hundreds 9 1 upwards FRFIRM_PENEM_g Università Politecnica delle Marche Capocasa et al. 2008 8.0 medium red TREXPOS_TREXPOS0-1M_0-1TREXPOSscale RosBREED GDR 1 dull Pincot, et al. RFOF_RFOF1-5M_1-5RESISTscale UC Davis RPF_RPF0-5M_0-5RESISTscale RosBREED GDR 5 globose-conic RosBREED GDR SKINGLOSS_SKINGLOSSM_1-9GLOSSscale even or very slightly uneven 1 highly susceptible/severe symptoms 1 RPCRR_RPC%M_% USDA RosBREED PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale GDR GDR RosBREED FRFLAV_FRFLAVM_1-9FRFLAVscale light orange 3 3 raised USDA FRRP_FRRPM_Ratio 2.5 very symptomatic very glossy 9 mildly symptomatic 2.0 Università Politecnica delle Marche Capocasa et al. 2008 SKINCOL_REFLECTLM_L* RUNNERDATE_FRUNNERM_date USDA UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale 2 outwards USDA FRLEN_FRLENM_mm STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale USDA medium 5 UPOV FLDIA_FLDIAM_cm 3 conic-wedge 3 calyx attachment is even with fruit FRSHAPE_FRSHAPEGGM_TXT USDA USDA Pinkerton & Finn RMH_RMHM_1-5RMHscale 1 very soft RUNNERNUM_RUNNERNUMCT_count USDA GDR FRpH_pHM_pH RosBREED oblate 7.0 TRUSSSIZ_TRUSSCOUNTM_ave RosBREED GDR USDA FRYIELD_FRYIELD1-4M_1-4FRYIELDscale 1 highly resistant/no symptoms RosBREED GDR ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale white 1 FLDATE100_FLDATE100M_FLDATE100date USDA small 3 not uniform/malformed 1 USDA ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq 9 plant has stamens CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale USDA very small to small 2 cordiform 7 greenish 1 GDR ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale RosBREED susceptible S RNEOP_RNEOP0-6M_0-6RESISTscale University of North Carolina Fernandez et al. very high 9.0 CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale UPOV very resistant, healthy 1.0 6.0 medium to large FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale UPOV no fruit set 1 partially remontant 2 UPOV TRUSSSIZ_TRUSSM_1-9scale 5.0 divides right before fruit acronym 3 same level 1 does not remove 7.0 large yellow 2 reniform 1 RosBREED GDR FRFLCOL_FRFLCOLM_1-9FRFLCOLscale pedicle breaks well above calyx 7 TotalPhenolics_F-CM_mg/L_GallicAcidEQ GDR RosBREED very large 9.0 Chandra et al. 2021 University of Florida SCG_SCGWKM_R-Scategory late 7 RosBREED GDR CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale USDA RVD_RVD%_% RMYFR_RMYFRM_% USDA Università Politecnica delle Marche Capocasa et al. 2008 TITA_TITAM_TITAMeqNAOH/100g 1 highly resistant/no symptoms globose 3 divides 50% from crown 3 RosBREED GDR HARVDATE_HARVDATEM_date 4 divides 75% from crown no fruit 1 RosBREED GDR ECOGDDREQH_GDDHM_days 1 strongly below surface Y Stem breaks just above calyx, good for fresh market USDA FLDATE2_FLDATE2M_date 7 many 3 strongly uneven 4 globose highly susceptible/severe symptoms 9 CE_CROPEST_g USDA TITA_TITA0.5M_TITAMeq/100gCA USDA fingerling 6.0 GDR FRFIRM_FRFIRMM_1-9FRFIRMscale RosBREED 2 medium yield dead 1 1 very early 9 completely uniform/symmetrical and attractive 1 absent or very weak USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV yellowish white 2 highly resistant/no symptoms 1 Oregon State Vining, et al. RVD_RVD1-3M_1-3RESISTscale RosBREED FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale GDR 9 extremely vigorous 1 soft medium 5 RosBREED GDR CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale EVERBERIN_EVER0-1M_0-1EVERscale USDA level with fruit 2 sunken 1 dark 1 1 no fruit 3 breakage is inconsistent BOTRYTIS_RBCM_0-1BOTRYTISscale USDA upright 1 V variable, no consistent breaking habit spreading 1 Università Politecnica delle Marche Capocasa et al. 2008 FRAP_FRAPTROM_TroloxEQumoles/g FW RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale CPBR RPOAP_RPOAP1-9M_1-9RESISTscale USDA even 1 FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale UPOV blackish red 9 4 no fruit set 2 slightly below USDA DNARATIO_DNADAPIICFM_DNA/stdDNAILF 1 highly susceptible/severe symptoms blackish red 10.0 ECOGDDREQ_GDDFM_days RosBREED GDR RosBREED GDR FLEVAL_FLEVALM_0-1FLEVALscale LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW University of Delaware Biswas et al. 2019 1 divides close to crown no runners 1 5.0 orange red high density 1 3 early 1 not sweet, bad off-flavors 7.0 light red fully remontant 3 1.5 slightly symptomatic semi-upright 2 dead 3.0 9 erect calyx size smaller than fruit diameter 1 calyx attachment raised above shoulder (necked) 1 high 7 PLANTHABIT_PLHABM_1-9PLHABscale USDA 1 no flowers CPBR PLANTHABIT_PLHABM_1-3PLHABscale pink 6.0 strongly uneven 5 highly susceptible/severe symptoms 8 highly susceptible/severe symptoms 5 protruding 3 USDA PLOIDY_FCDAPIIBLM_x 1 conic 8.0 large to very large 9.0 dark red plant has no stamens 1 Strawberry ontology strongly above 5.0 FLPETALNUM_FLPETALNUMM_average USDA 8.0 circular 3 calyx size larger than fruit diameter 2 slightly below surface 3 spreading intermediate 5 4 medium orange 9.0 oblate 3 few pedicle breaks just above calyx 5 FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale USDA RosBREED GDR FRFRESHWT_FRFRESHWT5M_g small to medium 4 4 slightly above 7 many flowers fully reflexed 9 USDA TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq 3 low density 1 white above surface 4 entity oblong 5.0 wedged 9.0 1 high yield 9.0 very late 9 very easily removed divides at 25% from crown 2 resistant R DAU_RUNDAUM_count USDA globose-conic 5.0 3 few flowers attribute very small 1 highly resistant/no symptoms 9 9 complete fruit set 3 red 9 overcropped Ease of calyx removal. Calyx Calyx ease of removal CALYXREMOVE ease of removal Observe where the pedicle breaks during harvest. Pedicle breakage method Observe average peduncle length and record it. Peduncle length method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight method resistance to Colletotrichum acutatum anthracnose fruit rot RAFR Fruit Fruit resistance to Colletotrichum acutatum anthracnose fruit rot The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Skin color chromameter reflect chroma index method date % Observe the plant and record the date when at the end of flowering. Flower date last method Plant resistance to Pratylenchus penetrans root lesion nematode RPP The plant resistance to root lesion nematode Pratylenchus penetrans. Plant resistance to Pratylenchus penetrans root lesion nematode Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Fruit firmness method STAMENPRES Stamen presence Stamen presence The presence of stamens. Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Resistance to Meloidogyne hapla method Harvest and count the number of runners collected from a single crown on a single day in a specific location. Number of runners count method count 1-8 RESIST scale Reproductive factor Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Susceptibility to Diplocarpon earlianum method 0-1 BOTRYITIS scale Count the total number of daughter plants from a single plant harvested on a single day. Daughter plantlet count Perpetual flowering method Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Flower date 100% method Observe the plant and record the date when at peak flowering. A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. Titratable acidity 0.5 g citric acid method Achene position 1-4 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Flower date first method Observe the plant and record the date the first flowers open. Calyx plug Calyx Presence or absence of central plug after calyx removal. CALYXPLUG plug Number of runners 1-7 method Observe and rate the number of runners per plant. 1-9 FRFLCOL scale Runner length total RTL Runner total length The total length of runners from a single plant harvested on a single day at a specific location. Record the number of weeks of flowering after week 11 from the first week of bloom. Perpetual flowering RB method The pH of fruit. pH Fruit Fruit pH FRpH Presence of powdery mildew on fruit method Observe the presence of powdery mildew on ripe fruit. Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. RFOF Plant resistance to Fusarium oxysporum f. sp. fragariae wilt Plant resistance to Fusarium oxysporum f. sp. fragariae wilt The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. 1-9 PLHAB scale Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Fruit flesh color method Flower date first relative Flower date first relative FLDATE1REL Relative date of first open flowers for an individual, whole plant, or population. Fruit yield 1-9 O method Estimate fruit yield and record it. harvest date Record the Julian date when first fruit ripens at a specific location. Fruit harvest date HARVDATE Fruit 1-3 CALYXSZ scale 1-9 FLDATE1REL UPOV scale Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method Total anthocyanins TACY Fruit total anthocyanin content The total anthocyanins of fruit. LFTOTANTIOX Leaf total antioxidant content Leaf total antioxidant content Total antioxidant content of leaves. Fruit shape UPOV method Observe and rate average fruit shape on 3-5 fruit per genotype. 0-1 STAMPRES scale Plant fruit development Plant ECOGDDREQH Growing degree days to first harvest. fruit development Growing degree days harvest method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Pedicle breakage relative to the calyx. STEMBREAK Pedicle breaking habit Stem breaking Select 3 flower trusses, count the flowers and average the results. Number of flowers per truss count viable The percentage of achenes that are viable. Achene Percentage of viable achenes ACHENEVIABLE Fruit development Fruit Number of days from flowering to ripe fruit. development FRDEV Ferric reducing antioxidant power trolox method Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). The total number of daughter plantlets per plant harvested on a single day at a specific location. Runner daughters daughters Runner DAU The weight of fresh fruit. Fruit fresh weight FRFRESHWT Fruit fresh weight The toughness of skin. SKINTOUGH Skin toughness Skin toughness SDE Plant susceptibility to leaf scorch caused by Diplocarpon earliana Plant The plant susceptibility to leaf scorch caused by Diplocarpon earliana susceptibility to Diplocarpon earliana 1-9 STAMENHGHT scale susceptibility to Phytophthora fragariae red stele root rot Plant susceptibility to Phytophthora fragariae red stele root rot The plant susceptibility to red stele root rot caused by Phytophthora fragariae. Plant SPF First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface Plant resistance to Mycosphaerella fragariae leaf spot resistance to Mycosphaerella fragariae leaf spot Plant RMYFR The plant resistance to Mycosphaerella fragariae. 1-9 FRYIELD Ordinal scale g The position of the calyx attachment relative to the fruit shoulder. position Calyx position CALYXPOS Calyx 1-9 FRSHAPE RB scale mg/L Gallic Acid EQ The plant resistance to powdery mildew caused by Podosphaera aphanis. Plant resistance to Podosphaera aphanis powdery mildew Plant RPOAP resistance to Podosphaera aphanis powdery mildew The weight of fresh fruit. Fruit fresh weight largest LFRW Largest fruit weight Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Susceptibility to Mycosphaerella fragariae method The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. SCG susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant resistance to necrosis caused by Neopestalotiopsis sp. Plant resistance to necrosis caused by Neopestalotiopsis sp. The plant resistance to necrosis caused by Neopestalotiopsis sp. Ferric reducing antioxidant power FSH method Plant RNEOP Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). FRYIELD Plant fruit yield Plant fruit yield The fruit yield of one individual. Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR 1-5 method Fruit ripening trait The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA Fruit FRRP ripening trait Pedicle length length Stem length of primary fruit from calyx to truss. Pedicle PEDICELLEN Observe the plant and record the relative flowering of the first open flowers. Flower date first relative method The plant resistance to verticillium wilt caused by Verticillium dahliae. Plant resistance to Verticillium dahliae wilt Plant resistance to Verticillium dahliae wilt RVD The degree of erectness of the leaves. PLANTHABIT Plant habit Plant habit Observe presence of anthers and record it. Presence of stamens 0-1 method Titratable acidity NaOH method Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. STAMENHGHT Stamen height Stamen height The height of the stamens relative to the receptacles. PLANTHT Plant height Plant Height of tallest trifoliate leaf. height DNA/std DNA_ILF Plant susceptibility to Phomopsis obscurans leaf blight Plant susceptibility to Phomopsis obscurans leaf blight SPO The plant susceptibility to leaf blight caused by Phomopsis obscurans. Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-3 method Fruit total water soluble solid content The soluble solids content of fruit. Fruit SSC total water soluble solid content Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae % method presence of grey mold BOTRYTIS The presence of grey mold caused by Botrytis cinerea on the plant or fruit. Indication of resistance to Botrytis Plant 1-3 CALYXREFLX UPOV scale Petal number average method Count the number of petals on 10 flowers and average the results Calyx reflexion CALYXREFLX Reflexion of the calyx relative to the fruit shoulders. Calyx reflexion DNARATIO Plant DNA ratio DNA ratio Plant Ratio of sample DNA to standard DNA. 0-5 SUSCEP scale g/100g Achene position ACHENEPOS The postion of achene relative to the fruit surface. position Achene Julian date of peak flowering for an individual, whole plant, or population. date 100% Flower date 100% Flower FLDATE100 RDE Plant resistance to Diplocarpon earlianum leaf scorch resistance to Diplocarpon earlianum leaf scorch The plant resistance to leaf scorch caused by Diplocarpon earliana. Plant Plant resistance to Phytophthora cactorum crown rot Plant resistance to Phytophthora cactorum crown rot The plant resistance to crown rot caused by Phytophthora cactorum. RPCCR mg/L Pg-3-gluc kg−1 The plant susceptibility to verticillium wilt caused by Verticillium dahliae. SVD susceptibility to Verticillium dahliae wilt Plant susceptibility to Verticillium dahliae wilt Plant 0-1 EVER scale x resistance to Phomopsis obscurans leaf blight The plant resistance to leaf blight caused by Phomopsis obscurans. RPO Plant resistance to Phomopsis obscurans leaf blight Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Folin-Ciocalteu method FRFIRM The firmness of fruit flesh. Fruit flesh firmness flesh firmness Fruit ECOGDDREQ Plant flower development Plant Growing degree days to first flowering. flower development 1-9 FRFIRM scale Julian date of first open flowers for an individual, whole plant, or population. FLDATE1 date first Flower date first Flower cm Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. Depth of fruit flesh color Susceptibility to Podosphaera aphanis method Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. Plant The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. SMYFR susceptibility to Mycosphaerella fragariae leaf spot Plant susceptibility to Mycosphaerella fragariae leaf spot Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Resistance to Podosphaera aphanis % method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Fruit ripen days Number of days from flowering to ripe fruit. FRD development Fruit The plant resistance to verticillium wilt caused by Verticillium dahliae. Plant resistance to Verticillium dahliae wilt RVD Plant resistance to Verticillium dahliae Plant Plant resistance to Meloidogyne hapla root knot nematode The plant resistance to root knot nematode Meloidogyne hapla. RMH resistance to Meloidogyne hapla root knot nematode 1-9 PLANTVIGOR scale Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis 1-9 method Skin color SKINCOL Skin Skin color on the sun side of a fully ripe fruit. color Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. Resistance to Colletotrichum acutatum anthracnose fruit rot % method 1-9 STAMPRES scale Calyx size The size of the calyx. CALYXSZ Calyx size Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-3 method Fruit fresh weight 1 method Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Resistance to Verticillium dahliae scale 1-9 1-9 RESIST scale ferric reducing antioxidant power Ferric reducing antioxidant power of fruit. Fruit ferric reducing antioxidant power Fruit FRAP 1-3 ACHENEPOS scale Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method color Skin SKINCOL Skin color on the sun side of a fully ripe fruit. Skin color The flavor of fruit. FRFLAV Fruit flavor Fruit flavor 1-3 RESIST scale Stamen height method Observe and rate the height of the stamens relative to the receptacle at flower opening. Average flower petal number. petal number Flower Flower petal number FLPETALNUM Harvest and record the largest fruit over the harvest season. Fruit fresh weight largest method Plant height method Measure the tallest trifoliate leaf from crown to base of leaflets. Observe and rate the number of runners per plant. Number of runners 1-9 method flesh color The color of fruit flesh. Fruit FRFLCOL Fruit flesh color Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Susceptibility to Phomopsis obscurans method Leaf pubescence The density of trichomes on the leaf surface. pubescence Leaf LFPUBESC Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae 0-5 method Runner total length method Harvest all runners on a single day and measure the total length. Fruit pH 2 method Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Fruit weight The weight of fresh fruit. 5FRW fresh weight Fruit 1-10 SKINCOL UPOV scale Fruit presence of powdery mildew PPMF Fruit presence of powdery mildew The presence of powdery mildew on fruit. R-S category Observe the plant weekly and record if it is flowering. Weekly flowering evaluation Truss exposure 1-5 method Observe the truss location relative to the foliage canopy and record it. 0-1 PMF scale Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. Percentage of viable achenes method The color of achene on the sun side of a fully ripe fruit. Achene ACHENECOL Achene color color resistance to Phytophthora cactorum root rot Plant resistance to Phytophthora cactorum root rot The plant resistance to root rot caused by Phytophthora cactorum. Plant RPCRR Fruit The total phenolic acids of fruit. total phenolic acids content TPHE Fruit total phenolic acids content Presence of stamens 1-9 method Observe presence of anthers and record it. Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. SSC:TA method Fruit total anthocyanin content The total anthocyanins of fruit. Fruit total anthocyanin content ATCYN Fruit appearance method Observe fruit appearance from not uniform to completely uniform. 1-9 FRYIELD scale Plant PLANTVIGOR Plant vigor The vigor of plant. vigor fruit yield The amount of harvested fruit per plant per season. Crop estimate Plant CE Harvest date Record the Julian date when first fruit ripens at a specific location. harvest date HD Fruit Observe and rate the fruit skin color. Skin color 1-10 method Fruit yield 1-4 method Estimate fruit yield and record it. 0-1 TREXPOS scale PLOIDY Plant ploidy The number of sets of chromosomes in the nucleus. Plant ploidy Observe and rate average fruit shape on 5 fruit per genotype. Fruit shape GG method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days flowering method 1-9 FRSHAPE UPOV scale Fruit firmness penetrometer method Measure fruit firmness in grams using a hand-held penetrometer with a piston. GG scale Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Flower Weekly flower evaluation FLEVAL development The weekly development of flowers. Susceptibility to Colletotrichum gloeosporioides weekly method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. Fruit fresh weight 5L method Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants Resistance to Neopestalotiopsis sp. scale 0-6 set Fruit FRUITSET The effeciency of fruit set at a specific location. Fruit set uniformity of color Fruit Fruit uniformity of color. FRCOLORUNIF Fruit uniformity of color length Epipodium Stem length of primary fruit from calyx to truss. Epipodium length EPILGT Observe the truss location relative to the foliage canopy and record it. Truss exposure 0-1 method mm 1-3 ACHENECOL UPOV scale FLDATE2 date Fruit drip loss drip loss Fruit Percent weight lost when frozen berries are thawed. DRIP LOSS resistance to Macrophomina phaseolina charcoal rot Plant RMaPh Plant resistance to Macrophomina phaseolina charcoal rot The plant resistance to charcoal rot caused by Macrophomina phaseolina. Record the number of days from first flowering to ripe fruit. Fruit development method Fruit Fruit shape FRSHAPE shape The shape of fruit. 1-4 PERPFL UPOV scale Observe the number of flowers per inflorescence. Number of flowers per truss estimation scale_of 1-9 SUSCEP scale Observe fruit color uniformity from even or very slighly unevento strongly uneven. Color uniformity method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Skin color chromameter reflect L* brightness method 1-9 FRFLAV scale Skin The glossiness of skin. gloss Skin gloss SKINGLOSS Plant habit scale method Observe the plant and assess the erectness or spreading of the leaves along the ground. Truss The position of the truss relative to the foliage. Truss exposure exposure TREXPO length PEDUNCLEN The length of peduncle. Peduncle length Peduncle Susceptibility to Phytophthora fragariae method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. 1-4 ACHENEPOS UPOV scale Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-3 method Calyx plug method Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx reflexion method Observe the plant and record the reflexion of the calyx relative to the fruit shoulders Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. Soluble solids method Calyx position 1-5 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina % method Fruit Depth of fruit flesh color. FRFLCOLDEPTH flesh depth of color Fruit flesh depth of color 1-9 FRSHAPUNI GRIN scale Resistance to Mycosphaerella fragariae method Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. First runner date method Observe the plant and record the Julian date the first runners emerge at a specific location. titratable acidity TITA The titratable acidity content of fruit. Fruit Fruit titratable acidity Plant susceptibility to Podosphaera aphanis The plant susceptibility to powdery mildew caused by Podosphaera aphanis. Plant susceptibility to Podosphaera aphanis powdery mildew SPOAP Resistance to Verticillium dahliae 1-3 method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Measure the diameter of the flowers at fully open Flower diameter method Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method The diameter of flower at fully open. Flower diameter Flower FLDIA diameter resistance to Phytophthora fragariae red stele root rot Plant resistance to Phytophthora fragariae red stele root rot Plant RPF The plant resistance to red stele root rot caused by Phytophthora fragariae. 1-5 TREXPOS UPOV scale meters Resistance to Phytophthora fragariae % method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Everbearing flowering method Observe the presence of flowers during each week in July, August and September. Plant Plant bearing type Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. BEARINGTYPE bearing type Meq NaOH/100g Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Ease of calyx removal method FRpH Epipodium measurement method Measure the length of the epipodium of the primary fruit from the calyx to the truss. Trolox EQ μmoles/g FW FRSHAPEUNIF Fruit shape uniformity shape uniformity Fruit Fruit shape uniformity. Stembreak txt 1-9 FRUITSET scale Fruit FRFIRM Fruit flesh firmness The firmness of fruit flesh. flesh firmness Harvest date method Record the Julian date when first fruit ripens at a specific location. Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Pg-3-gluc method Observe the number of fruit that a single plant sets in a specific location. Fruit set method Resistance to Diplocarpon earlianum method Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. The Julian date of appearance of the first runners. Runner First runner date RUNNERDATE first emergence Runner number RUNNERNUM Runner number The number of runners. 1-9 FLDIA UPOV scale FRCOL1 first color appearance Appearance of first color on fruit. Fruit Appearance of first color on fruit 1-3 FRCOLUNI scale 0-1 CALPLUG scale Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Flower diameter UPOV method Chroma Index Length of fruit from calyx to tip. Fruit length Fruit length FRLEN days Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina method Observe the presence of grey mold on the plant or fruit. Resistance to Botrytis cinerea method 1-5 CALYXPOS scale 1-5 RESIST scale 1-9 CALYXREM scale Resistance to Phytophthora cactorum CR % method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. The titratable acidity content of fruit. TITA Fruit Fruit titratable acidity titratable acidity Plant EVERBERIN Whether or not the plant has an everbearing habit bearing type Everbearing Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. Calyx size method 1-9 CALYXREFLX scale Ratio Meq/100g citric acid Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Drip loss method 1-5 PEDUNCLEN scale Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Susceptibility to Verticillium dahliae 1-5 method TRUSSSIZ Truss Number of flowers per truss Truss size size 1-5 SUSCEP scale Crop estimate method Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. 1-3 PLHAB scale Pedicle breakage relative to the calyx. breaking habit Pedicle breaking habit Pedicle PEDICLEBREAK grams Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. Fruit pH method Fruit yield 1-9 method Estimate fruit yield and record it. mg/L Cy-3-gluc Eq FLDATE1 Date Plant vigor method Observe plant vigor of the two clones and record the average. Skin color 1-9 method Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Fruit flavor method Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. DNA ration determination using DAPI with internal standard = iceberg lettuce method 1-5 RMH scale 1-9 FRGLOSS scale 0-6 RESIST scale Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. 1-7 PEDBRK scale DNA/std DNA_IBL average Total anthocyanins Cy-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. FLDATE2 Flower Julian date of last flowering for an individual, whole plant, or population. Flower date last date last 1-5 FRCOLUNI scale Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-9 method Measure the length of the fruit from the calyx to the tip and record in mm. Fruit length method Julian date of first open flowers for an individual, whole plant, or population. date first Bloom date first BDF Flower 1-4 FRYIELD scale Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape RB method 1-9 ACHENECOL scale 1-3 pubescence scale Weigh total fruit yield and record it. Fruit yield total weight method 0-7 TRUSS scale Measure the length of the pedicel of the primary fruit from the calyx to the truss. Pedicel measurement method 1-3 CALYXPOS UPOV scale FLDATE100 date FSH EQ μmoles/g fruit 1-9 SKINTOUGH scale Resistance to Verticillium dahliae % method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Leaf total antioxidant assay method Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Titratable acidity citric acid method Luminance 1-9 RUNNERNUM scale 1-7 RUNNERNUM scale method_of 1-9 SKINCOL scale 0-1 FLEVAL scale variable_of