7.0 large TRUSSSIZ_TRUSSCOUNTM_ave GDR RosBREED USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg Pinkerton & Finn USDA RMH_RMHM_1-5RMHscale CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale UPOV USDA FRUITSET_FRSETM_1-9FRUITSETscale USDA RPOAP_RPOAP1-9M_1-9RESISTscale 4 above surface DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL USDA USDA STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale 9.0 oblate 6.0 pink Biswas et al. 2019 University of Delaware LFPUBESC_LFPUBESCM_1-3LFPUBESCscale RMaPh_ RMaPh%M_ % USDA Zurn, et al. PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale GDR RosBREED TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA GDR RosBREED FLEVAL_FLEVALM_0-1FLEVALscale DAU_RUNDAUM_count USDA 5.0 oblong DNARATIO_DNADAPIICFM_DNA/stdDNAILF USDA USDA EVERBERIN_EVER0-1M_0-1EVERscale GDR RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale GDR RosBREED FRFRESHWT_FRFRESHWT5M_g 7 many tough 9 oblate 7.0 USDA PLOIDY_FCDAPIICFM_x 6.0 medium to large USDA FRDEV_FRDEVM_days GDR RosBREED SKINGLOSS_SKINGLOSSM_1-9GLOSSscale Pinkerton & Finn USDA RPP_RPPM_Rf NEM-RF_RPPM_Rf GDR RosBREED FRpH_pHM_pH 3 downwards very soft 1 entity highly resistant/no symptoms 1 RosBREED CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale GDR FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale CPBR very large 9.0 very easily removed 9 Università Politecnica delle Marche Capocasa et al. 2008 FRFIRM_PENEM_g GDR CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale RosBREED USDA FLDATE1_FLDATE1M_FLDATE1date FRFIRM_FRFIRMM_1-9FRFIRMscale GDR RosBREED UC Davis Knapp, et al. RMaPh_ RMaPhM_1-5RESISTscale pedicle does not break 1 FRCOL1_FRCOL1M_date USDA ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV medium 5.0 RosBREED GDR ECOGDDREQ_GDDFM_days FRSHAPE_FRSHAPEGGM_TXT USDA strongly below surface 1 RPOAP_RPOAP%M_% USDA GDR RosBREED SMYFR_SMYFRM_1-9SUSCEPscale USDA LFRW_FRFRESHWLGM_g FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale UPOV conic 2 GDR RosBREED BEARINGTYPE_BEARINGRBM_count UPOV FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale SKINCOL_REFLECTCIXM_ChromaIndex Università Politecnica delle Marche Capocasa et al. 2008 USDA FRLEN_FRLENM_mm 3 strongly uneven GDR PPMF_PPMFM_0-1PMFscale RosBREED RPF_RPF%M_% USDA 5 medium USDA HD_FIRSTHARVM_date GDR PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale RosBREED 5 medium V variable, no consistent breaking habit 1 sunken Pincot et al. 2020 University of California, Davis RVD_RVD1-9M_1-9RESISTScale 1 no fruit low 3 plant has no stamens 1 ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq RosBREED GDR RosBREED GDR STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale cordiform 7 STEMBREAK_PEDBRKM_text USDA no runners 1 RUNNERDATE_FRUNNERM_date USDA CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale UPOV 1 high yield medium density 2 UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale RosBREED GDR GDR RosBREED FRFLCOL_FRFLCOLM_1-9FRFLCOLscale BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale UPOV USDA FRYIELD_FRYIELDTWM_g RUNNERNUM_RUNNERNUMCT_count USDA 1 reniform USDA PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale GDR RosBREED FRFLCOLDEPTH_FRCOLDEPTHM_% PEDICELLEN_PEDICELLENM_cm USDA ovate 4 5.0 strongly above slightly uneven 3 8.0 large to very large TotalPhenolics_F-CM_mg/L_GallicAcidEQ GDR RosBREED RFOF_RFOF%M_% Zurn, et al. USDA RosBREED RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale GDR UPOV FLDIA_FLDIAM_cm 9 fully reflexed FRAP_FRAPTROM_TroloxEQumoles/g FW Università Politecnica delle Marche Capocasa et al. 2008 medium red 8.0 USDA RTL_RUNTOTLENM_m 3 calyx size larger than fruit diameter slightly below 2 GDR RosBREED FRYIELD_FRYIELD1-9M_1-9FRYIELDscale BOTRYTIS_RBCM_0-1BOTRYTISscale USDA UC Davis RFOF_RFOF1-5M_1-5RESISTscale Pincot, et al. highly susceptible/severe symptoms 9 USDA STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale yellowish white 2 Feldmann, et al. SVD_SVD1-5M_1-5SUSCEPscale UC Davis GDR RosBREED TITA_TITAM_TITAMeq/100gCA 3 early 5.0 globose-conic 8 highly susceptible/severe symptoms 9 completely uniform/symmetrical and attractive RMYFR_RMYFRM_% USDA ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale GDR RosBREED dark red 9.0 divides right before fruit 5.0 raised 3 3 small does not remove 1 Eikemo, et al. RPCCR_RPCCR1-8M_1-8RESISTscale NCRI RosBREED SPF_SPFM_0-5SUSCEPscale GDR UPOV FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale Università Politecnica delle Marche Capocasa et al. 2008 FRAP_FRAPFSHM_FSHEQumoles/g fruit spreading 3 RPF_RPF0-5M_0-5RESISTscale GDR RosBREED 7 high 1 soft day neutral 4 9 erect 9 highly resistant/no symptoms USDA FRD_FRRIPEM_days GDR RosBREED HARVDATE_HARVDATEM_date inserted 1 highly resistant/no symptoms 1 medium yield 2 GDR RosBREED DRIPLOSS_DRIPLOSSM_g 7 many flowers medium number of flowers 5 RNEOP_RNEOP0-6M_0-6RESISTscale University of North Carolina Fernandez et al. PLANTHABIT_PLHABM_1-3PLHABscale CPBR same level 3 high density 1 3 cordate FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale USDA very high 9.0 USDA RPO_RPOM_% 4 medium orange FRYIELD_FRYIELD1-4M_1-4FRYIELDscale USDA Università Politecnica delle Marche Capocasa et al. 2008 TITA_TITAM_TITAMeqNAOH/100g ACHENEVIABLE_ACHVIAM_% RosBREED GDR FRRP_FRRPM_Ratio USDA 7 late USDA FLDATE100_FLDATE100M_FLDATE100date RPCRR_RPC%M_% USDA 1 not reflexed TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale UPOV University of Florida Salinas et al. 2019 FRRAFR_FRAFR%M_% PLOIDY_FCDAPIIBLM_x USDA 9 very light brown or green TREXPOS_TREXPOS0-1M_0-1TREXPOSscale GDR RosBREED 3 fully remontant 2.5 very symptomatic long conic 1 1 strongly below GDR RosBREED CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale GDR FRFLAV_FRFLAVM_1-9FRFLAVscale RosBREED 5 calyx attachment is sunken below shoulder 1 absent or very weak orange red 5.0 GDR RosBREED SDE_SDEM_1-9SUSCEPscale USDA ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq RVD_RVD%_% USDA RPCCR_RPCCR%M_% USDA 5 intermediate FLPETALNUM_FLPETALNUMM_average USDA 2 long conic Università Politecnica delle Marche Capocasa et al. 2008 SKINCOL_REFLECTLM_L* 9 blackish red Jiménez, et al. UC Davis RPCCR_RPCCR1-5M_1-5RESISTscale 1 not remontant FLDIA_FLDIAM_1-9FLDIAUPOVscale UPOV circular 8.0 1 upright rhombic 6.0 9 very firm USDA EPILGT_EPILGTM_cm very late 9.0 CALYXSZ_CALYXSZM_1-3CALYXSZscale GDR RosBREED 6.0 fingerling 1 not sweet, bad off-flavors FLDATE2_FLDATE2M_date USDA calyx attachment is even with fruit 3 2.0 mildly symptomatic R resistant 3 low yield USDA CE_CROPEST_g GDR RosBREED SKINCOL_SKINCOL1-9M_1-9SKINCOLscale highly resistant/no symptoms 9 CPBR RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale few flowers 3 3 conic-wedge acronym calyx size equal to fruit diameter 2 1.5 slightly symptomatic USDA PLANTHABIT_PLHABM_1-9PLHABscale low density 3 2 slightly below surface GDR RosBREED SPO_SPOM_1-9SUSCEPscale few 3 BLDATE1_FLDATE1M_FLDATE1date USDA Y** stem breaks well above calyx 1 white Vining, et al. RVD_RVD1-3M_1-3RESISTscale Oregon State level with fruit 2 UPOV TRUSSSIZ_TRUSSM_1-9scale hundreds 9 4 divides 75% from crown slightly above 4 TITA_TITA0.5M_TITAMeq/100gCA USDA 3 light orange SPOAP_SPOAPM_1-9SUSCEPscale RosBREED GDR UPOV SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale 1 white globose-conic 5 4 globose pedicle breaks just above calyx 5 2 partially remontant upwards 1 stamens shorter than receptacle 1 GDR RosBREED ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale FRpH_pH2M_pH USDA strongly uneven 5 9 dark red 2 divides at 25% from crown 9 complete fruit set 1 calyx attachment raised above shoulder (necked) 1 even very early 1 breakage is inconsistent 3 no fruit set 4 3 red divides close to crown 1 very small 1 RDE_REDM_1-9RESISTscale USDA 1 highly susceptible/severe symptoms very glossy 9 9 very sweet, no off-flavors 1 spreading no fruit set 1 Biswas et al. 2019 University of Delaware LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW globose 3 9 stamens taller than receptacle highly susceptible/severe symptoms 1 SSC_SSCM_SSCg/100g GDR RosBREED FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale USDA 2 semi-upright 1 no flowers 10.0 blackish red light red 7.0 level with surface 3 3.0 dead 4 small to medium ECOGDDREQH_GDDHM_days GDR RosBREED N stem does not break, berry separates from calyx easily 1 dead S susceptible extremely vigorous 9 very small to small 2 pedicle breaks well above calyx 7 not uniform/malformed 1 Y Stem breaks just above calyx, good for fresh market even 2 USDA 5FRW_FRFRESHWT5LM_g 1 even or very slightly uneven Chandra et al. 2021 University of Florida SCG_SCGWKM_R-Scategory 1.0 very resistant, healthy calyx size smaller than fruit diameter 1 plant has stamens 9 conic 1 PLANTHT_PLANTHTM_cm USDA 1 highly resistant/no symptoms attribute outwards 2 2 slightly uneven CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale USDA divides 50% from crown 3 greenish 1 highly susceptible/severe symptoms 5 no fruit 1 2 yellow Strawberry ontology 3 protruding pinkish white 1 overcropped 9 9.0 wedged 1 dark dull 1 Achene viable Percentage of viable achenes The percentage of achenes that are viable. ACHENEVIABLE DNA ration determination using DAPI with internal standard = iceberg lettuce method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Total anthocyanins Pg-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Record the number of days from first flowering to ripe fruit. Fruit development method Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Fruit pH 2 method Susceptibility to Colletotrichum gloeosporioides weekly method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Relative date of first open flowers for an individual, whole plant, or population. Flower Flower date first relative FLDATE1REL date first relative RPCRR The plant resistance to root rot caused by Phytophthora cactorum. Plant Plant resistance to Phytophthora cactorum root rot resistance to Phytophthora cactorum root rot Fruit yield 1-9 method Estimate fruit yield and record it. SVD Plant The plant susceptibility to verticillium wilt caused by Verticillium dahliae. Plant susceptibility to Verticillium dahliae wilt susceptibility to Verticillium dahliae wilt Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Record the Julian date when first fruit ripens at a specific location. Harvest date HD harvest date Fruit Harvest date method Record the Julian date when first fruit ripens at a specific location. Plant vigor method Observe plant vigor of the two clones and record the average. Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. DNA/std DNA_ILF 1-9 FRYIELD Ordinal scale The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. susceptibility to Mycosphaerella fragariae leaf spot Plant susceptibility to Mycosphaerella fragariae leaf spot SMYFR Plant Skin color on the sun side of a fully ripe fruit. Skin color SKINCOL color Skin grams Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. Susceptibility to Phytophthora fragariae method R-S category Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Ease of calyx removal method 1-9 SKINCOL scale first emergence Runner RUNNERDATE The Julian date of appearance of the first runners. First runner date Achene color 1-3 method Observe the average achene color on 3-5 fruit per genotype and record it. mg/L Gallic Acid EQ cm % 1-3 RESIST scale Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method FSH EQ μmoles/g fruit 1-9 PLANTVIGOR scale Resistance to Macrophomina phaseolina method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Fruit flesh color method Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. Susceptibility to Podosphaera aphanis method Titratable acidity citric acid method Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. FRYIELD Plant Plant fruit yield The fruit yield of one individual. fruit yield The length of peduncle. Peduncle length Peduncle length PEDUNCLEN Leaf total antioxidant content LFTOTANTIOX total antioxidant content Total antioxidant content of leaves. Leaf Observe the truss location relative to the foliage canopy and record it. Truss exposure 0-1 method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae % method Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Resistance to Diplocarpon earlianum method 0-6 RESIST scale 1-5 PEDUNCLEN scale 1-3 pubescence scale Calyx reflexion reflexion Calyx Reflexion of the calyx relative to the fruit shoulders. CALYXREFLX Measure the diameter of the flowers at fully open Flower diameter method Observe the presence of flowers during each week in July, August and September. Everbearing flowering method Observe the plant and record the date when at peak flowering. Flower date 100% method Petal number average method Count the number of petals on 10 flowers and average the results Plant BEARINGTYPE bearing type Plant bearing type Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. 1-3 FRCOLUNI scale Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Susceptibility to Verticillium dahliae 1-5 method Fruit flesh firmness FRFIRM flesh firmness The firmness of fruit flesh. Fruit Plant resistance to Meloidogyne hapla root knot nematode RMH Plant resistance to Meloidogyne hapla root knot nematode The plant resistance to root knot nematode Meloidogyne hapla. Plant susceptibility to leaf scorch caused by Diplocarpon earliana SDE The plant susceptibility to leaf scorch caused by Diplocarpon earliana Plant susceptibility to Diplocarpon earliana DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. size The size of the calyx. CALYXSZ Calyx Calyx size Fruit presence of powdery mildew Fruit The presence of powdery mildew on fruit. PPMF presence of powdery mildew Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method 1-9 CALYXREM scale Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. SPOAP Plant The plant susceptibility to powdery mildew caused by Podosphaera aphanis. Plant susceptibility to Podosphaera aphanis powdery mildew susceptibility to Podosphaera aphanis Peduncle length method Observe average peduncle length and record it. The total phenolic acids of fruit. TPHE Fruit total phenolic acids content Fruit total phenolic acids content Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Skin color chromameter reflect chroma index method 1-4 ACHENEPOS UPOV scale Measure the length of the epipodium of the primary fruit from the calyx to the truss. Epipodium measurement method FRpH Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Skin color chromameter reflect L* brightness method Measure the length of the pedicel of the primary fruit from the calyx to the truss. Pedicel measurement method Number of runners count method Harvest and count the number of runners collected from a single crown on a single day in a specific location. 5FRW fresh weight The weight of fresh fruit. Fruit weight Fruit Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight method 1-9 FRSHAPE RB scale The plant resistance to Mycosphaerella fragariae. RMYFR Plant resistance to Mycosphaerella fragariae leaf spot Plant resistance to Mycosphaerella fragariae leaf spot Fruit pH method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. PLOIDY Plant ploidy The number of sets of chromosomes in the nucleus. Plant ploidy days Fruit flesh depth of color Fruit Depth of fruit flesh color. flesh depth of color FRFLCOLDEPTH SPF susceptibility to Phytophthora fragariae red stele root rot Plant The plant susceptibility to red stele root rot caused by Phytophthora fragariae. Plant susceptibility to Phytophthora fragariae red stele root rot Measure fruit firmness in grams using a hand-held penetrometer with a piston. Fruit firmness penetrometer method pubescence The density of trichomes on the leaf surface. Leaf pubescence LFPUBESC Leaf Estimate fruit yield and record it. Fruit yield 1-4 method Plant The plant resistance to powdery mildew caused by Podosphaera aphanis. RPOAP Plant resistance to Podosphaera aphanis powdery mildew resistance to Podosphaera aphanis powdery mildew Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days flowering method Plant RPCCR The plant resistance to crown rot caused by Phytophthora cactorum. Plant resistance to Phytophthora cactorum crown rot resistance to Phytophthora cactorum crown rot Observe the plant weekly and record if it is flowering. Weekly flowering evaluation 1-7 RUNNERNUM scale Plant susceptibility to Phomopsis obscurans leaf blight SPO susceptibility to Phomopsis obscurans leaf blight The plant susceptibility to leaf blight caused by Phomopsis obscurans. Plant Whether or not the plant has an everbearing habit Plant Everbearing EVERBERIN bearing type x Total anthocyanins Cy-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. 1-9 SUSCEP scale height STAMENHGHT Stamen Stamen height The height of the stamens relative to the receptacles. count Estimate fruit yield and record it. Fruit yield 1-9 O method Resistance to Podosphaera aphanis 1-9 method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. FRSHAPEUNIF shape uniformity Fruit Fruit shape uniformity Fruit shape uniformity. drip loss Fruit DRIP LOSS Fruit drip loss Percent weight lost when frozen berries are thawed. meters 1-4 PERPFL UPOV scale g PLANTHT Height of tallest trifoliate leaf. height Plant height Plant 1-5 SUSCEP scale The plant resistance to necrosis caused by Neopestalotiopsis sp. Plant resistance to necrosis caused by Neopestalotiopsis sp. RNEOP resistance to necrosis caused by Neopestalotiopsis sp. Plant Ferric reducing antioxidant power FSH method Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). Plant ECOGDDREQ Plant flower development flower development Growing degree days to first flowering. Fruit FRCOLORUNIF uniformity of color Fruit uniformity of color. Fruit uniformity of color Stembreak txt resistance to Colletotrichum acutatum anthracnose fruit rot RAFR The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. Fruit Fruit resistance to Colletotrichum acutatum anthracnose fruit rot 0-1 STAMPRES scale Epipodium length Epipodium EPILGT length Stem length of primary fruit from calyx to truss. Plant resistance to Macrophomina phaseolina charcoal rot Plant The plant resistance to charcoal rot caused by Macrophomina phaseolina. resistance to Macrophomina phaseolina charcoal rot RMaPh First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface Plant height method Measure the tallest trifoliate leaf from crown to base of leaflets. 1-3 ACHENEPOS scale TITA Fruit titratable acidity titratable acidity Fruit The titratable acidity content of fruit. Plant resistance to Phytophthora fragariae red stele root rot Plant The plant resistance to red stele root rot caused by Phytophthora fragariae. RPF resistance to Phytophthora fragariae red stele root rot SCG The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant Soluble solids method Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. Achene color The color of achene on the sun side of a fully ripe fruit. Achene color ACHENECOL Number of flowers per truss TRUSSSIZ Truss size size Truss Pedicle breakage method Observe where the pedicle breaks during harvest. 1-10 SKINCOL UPOV scale Fruit Fruit set set FRUITSET The effeciency of fruit set at a specific location. SSC:TA method Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. 1-3 PLHAB scale Luminance Average flower petal number. Flower petal number Flower petal number FLPETALNUM Number of runners 1-7 method Observe and rate the number of runners per plant. Presence of stamens 1-9 method Observe presence of anthers and record it. Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae % method Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Susceptibility to Diplocarpon earlianum method 0-1 EVER scale Skin Skin gloss gloss SKINGLOSS The glossiness of skin. Fruit development FRD Number of days from flowering to ripe fruit. Fruit ripen days Flower date first Julian date of first open flowers for an individual, whole plant, or population. date first FLDATE1 Flower Truss exposure The position of the truss relative to the foliage. exposure TREXPO Truss Indication of resistance to Botrytis Plant presence of grey mold BOTRYTIS The presence of grey mold caused by Botrytis cinerea on the plant or fruit. Plant resistance to Verticillium dahliae wilt The plant resistance to verticillium wilt caused by Verticillium dahliae. RVD resistance to Verticillium dahliae wilt Plant Number of runners 1-9 method Observe and rate the number of runners per plant. Fruit HARVDATE Record the Julian date when first fruit ripens at a specific location. harvest date Fruit harvest date Skin color 1-10 method Observe and rate the fruit skin color. Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. Fruit fresh weight 5L method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days harvest method Fruit firmness method Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Resistance to Colletotrichum acutatum anthracnose fruit rot % method Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. flesh color The color of fruit flesh. FRFLCOL Fruit flesh color Fruit 1-9 RUNNERNUM scale The toughness of skin. Skin Skin toughness SKINTOUGH toughness Plant PLANTHABIT The degree of erectness of the leaves. Plant habit habit Trolox EQ μmoles/g FW Resistance to Phytophthora cactorum CR % method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Leaf total antioxidant assay method Fruit length method Measure the length of the fruit from the calyx to the tip and record in mm. Fruit shape GG method Observe and rate average fruit shape on 5 fruit per genotype. The position of the calyx attachment relative to the fruit shoulder. position CALYXPOS Calyx Calyx position Observe the truss location relative to the foliage canopy and record it. Truss exposure 1-5 method Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. 1-9 PLHAB scale Ratio of sample DNA to standard DNA. Plant Plant DNA ratio DNARATIO DNA ratio Harvest and record the largest fruit over the harvest season. Fruit fresh weight largest method Fruit FRFLAV flavor The flavor of fruit. Fruit flavor Stamen height method Observe and rate the height of the stamens relative to the receptacle at flower opening. Fruit appearance method Observe fruit appearance from not uniform to completely uniform. 0-1 BOTRYITIS scale 1-9 FRFIRM scale 1-3 CALYXREFLX UPOV scale The plant resistance to verticillium wilt caused by Verticillium dahliae. RVD Plant resistance to Verticillium dahliae wilt Plant resistance to Verticillium dahliae resistance to Diplocarpon earlianum leaf scorch RDE The plant resistance to leaf scorch caused by Diplocarpon earliana. Plant resistance to Diplocarpon earlianum leaf scorch Plant 1-8 RESIST scale Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Susceptibility to Mycosphaerella fragariae method 1-9 STAMENHGHT scale 0-1 CALPLUG scale Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-4 method Observe the number of flowers per inflorescence. Number of flowers per truss estimation Skin color on the sun side of a fully ripe fruit. SKINCOL color Skin Skin color CALYXPLUG Calyx plug plug Presence or absence of central plug after calyx removal. Calyx 1-9 FLDATE1REL UPOV scale TITA titratable acidity Fruit The titratable acidity content of fruit. Fruit titratable acidity 1-3 CALYXSZ scale 1-9 FRUITSET scale GG scale Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Resistance to Macrophomina phaseolina % method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. mg/L Cy-3-gluc Eq average Resistance to Verticillium dahliae scale 1-9 Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. 1-9 FLDIA UPOV scale Achene position 1-3 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. The amount of harvested fruit per plant per season. Plant CE Crop estimate fruit yield 1-9 FRFLAV scale The total anthocyanins of fruit. Fruit total anthocyanin content ATCYN Fruit total anthocyanin content development The weekly development of flowers. Weekly flower evaluation FLEVAL Flower Titratable acidity 0.5 g citric acid method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis % method 1-5 RESIST scale 1-5 CALYXPOS scale 1-9 SKINTOUGH scale Observe presence of anthers and record it. Presence of stamens 0-1 method Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Flower diameter UPOV method position ACHENEPOS The postion of achene relative to the fruit surface. Achene position Achene Flower FLDIA Flower diameter diameter The diameter of flower at fully open. Folin-Ciocalteu method Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Resistance to Phytophthora fragariae % method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Verticillium dahliae 1-3 method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Pedicle Pedicle breaking habit Pedicle breakage relative to the calyx. PEDICLEBREAK breaking habit resistance to Phomopsis obscurans leaf blight RPO Plant resistance to Phomopsis obscurans leaf blight The plant resistance to leaf blight caused by Phomopsis obscurans. Flower date first method Observe the plant and record the date the first flowers open. length Pedicle length Stem length of primary fruit from calyx to truss. Pedicle PEDICELLEN Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method FLDATE2 Flower Flower date last Julian date of last flowering for an individual, whole plant, or population. date last FRLEN Fruit length Fruit Length of fruit from calyx to tip. length Flower date last method Observe the plant and record the date when at the end of flowering. date Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight 1 method Fruit flavor method Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Fruit shape UPOV method Observe and rate average fruit shape on 3-5 fruit per genotype. 0-1 TREXPOS scale Flower date 100% date 100% FLDATE100 Flower Julian date of peak flowering for an individual, whole plant, or population. The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA FRRP ripening trait Fruit Fruit ripening trait The presence of stamens. STAMENPRES Stamen presence presence Stamen Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Drip loss method 1-9 CALYXREFLX scale DNA/std DNA_IBL Reproductive factor Meq/100g citric acid Observe the plant and assess the erectness or spreading of the leaves along the ground. Plant habit scale method FRFRESHWT The weight of fresh fruit. Fruit Fruit fresh weight fresh weight Fruit development FRDEV Number of days from flowering to ripe fruit. development Fruit RPP resistance to Pratylenchus penetrans root lesion nematode Plant resistance to Pratylenchus penetrans root lesion nematode The plant resistance to root lesion nematode Pratylenchus penetrans. Plant Observe the plant and record the relative flowering of the first open flowers. Flower date first relative method Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-9 method Pedicle Stem breaking breaking habit STEMBREAK Pedicle breakage relative to the calyx. Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. Percentage of viable achenes method Calyx size method Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. DAU daughters The total number of daughter plantlets per plant harvested on a single day at a specific location. Runner Runner daughters Plant resistance to Fusarium oxysporum f. sp. fragariae wilt RFOF Plant The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. resistance to Fusarium oxysporum f. sp. fragariae wilt Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. Depth of fruit flesh color The firmness of fruit flesh. Fruit flesh firmness FRFIRM Fruit flesh firmness Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR 1-5 method Runner RTL Runner total length length total The total length of runners from a single plant harvested on a single day at a specific location. fruit development Plant fruit development Plant Growing degree days to first harvest. ECOGDDREQH Ferric reducing antioxidant power of fruit. Fruit ferric reducing antioxidant power ferric reducing antioxidant power Fruit FRAP 1-7 PEDBRK scale Runner total length method Harvest all runners on a single day and measure the total length. Resistance to Meloidogyne hapla method Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Perpetual flowering method 1-4 FRYIELD scale Observe the plant and record the Julian date the first runners emerge at a specific location. First runner date method SSC total water soluble solid content The soluble solids content of fruit. Fruit Fruit total water soluble solid content mg/L Pg-3-gluc kg−1 0-7 TRUSS scale FLDATE100 date variable_of Largest fruit weight fresh weight largest The weight of fresh fruit. Fruit LFRW FLDATE2 date Calyx ease of removal Ease of calyx removal. CALYXREMOVE Calyx ease of removal shape Fruit shape Fruit The shape of fruit. FRSHAPE Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Susceptibility to Phomopsis obscurans method Plant vigor Plant The vigor of plant. PLANTVIGOR vigor Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae 0-5 method Meq NaOH/100g Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Ferric reducing antioxidant power trolox method Resistance to Mycosphaerella fragariae method Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-3 method 1-9 FRSHAPUNI GRIN scale Chroma Index Color uniformity method Observe fruit color uniformity from even or very slighly unevento strongly uneven. Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Titratable acidity NaOH method Fruit yield total weight method Weigh total fruit yield and record it. 1-3 ACHENECOL UPOV scale mm 1-5 TREXPOS UPOV scale 1-9 FRSHAPE UPOV scale Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape RB method Calyx position 1-5 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. The total anthocyanins of fruit. Total anthocyanins total anthocyanin content Fruit TACY Flower BDF date first Bloom date first Julian date of first open flowers for an individual, whole plant, or population. Skin color 1-9 method Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Observe the presence of powdery mildew on ripe fruit. Presence of powdery mildew on fruit method Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx plug method 1-9 FRGLOSS scale 1-9 FRYIELD scale Observe the number of fruit that a single plant sets in a specific location. Fruit set method 1-9 ACHENECOL scale Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method 0-1 PMF scale Perpetual flowering RB method Record the number of weeks of flowering after week 11 from the first week of bloom. pH The pH of fruit. Fruit pH Fruit FRpH 1-9 RESIST scale 1-5 FRCOLUNI scale 1-9 STAMPRES scale Appearance of first color on fruit Fruit Appearance of first color on fruit. first color appearance FRCOL1 1-3 CALYXPOS UPOV scale Ratio Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. Runner RUNNERNUM The number of runners. Runner number number Resistance to Neopestalotiopsis sp. scale 0-6 Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants 1-9 FRFLCOL scale g/100g FLDATE1 Date method_of 1-5 RMH scale Daughter plantlet count Count the total number of daughter plants from a single plant harvested on a single day. Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Crop estimate method Calyx reflexion method Observe the plant and record the reflexion of the calyx relative to the fruit shoulders 0-1 FLEVAL scale scale_of 0-5 SUSCEP scale