FRRP_FRRPM_Ratio USDA LFPUBESC_LFPUBESCM_1-3LFPUBESCscale Biswas et al. 2019 University of Delaware 5 medium 7 many CPBR PLANTHABIT_PLHABM_1-3PLHABscale USDA STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale 2 medium density GDR FRYIELD_FRYIELD1-9M_1-9FRYIELDscale RosBREED 7 late 4 day neutral downwards 3 many flowers 7 7.0 oblate UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale 1 highly susceptible/severe symptoms Capocasa et al. 2008 SKINCOL_REFLECTLM_L* Università Politecnica delle Marche BOTRYTIS_RBCM_0-1BOTRYTISscale USDA GDR RosBREED PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale USDA RPOAP_RPOAP1-9M_1-9RESISTscale circular 8.0 spreading 3 USDA EPILGT_EPILGTM_cm FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale UPOV GDR RosBREED SKINCOL_SKINCOL1-9M_1-9SKINCOLscale 7.0 light red TITA_TITAM_TITAMeq/100gCA GDR RosBREED RosBREED CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale GDR FRRAFR_FRAFR%M_% Salinas et al. 2019 University of Florida 1 dead FRUITSET_FRSETM_1-9FRUITSETscale USDA highly susceptible/severe symptoms 1 USDA PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale UPOV FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale SVD_SVD1-5M_1-5SUSCEPscale UC Davis Feldmann, et al. FRCOL1_FRCOL1M_date USDA Capocasa et al. 2008 Università Politecnica delle Marche FRAP_FRAPFSHM_FSHEQumoles/g fruit CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale USDA 2.0 mildly symptomatic GDR RosBREED ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq RosBREED TREXPOS_TREXPOS0-1M_0-1TREXPOSscale GDR medium red 8.0 UC Davis Jiménez, et al. RPCCR_RPCCR1-5M_1-5RESISTscale GDR FRpH_pHM_pH RosBREED not remontant 1 ECOGDDREQH_GDDHM_days GDR RosBREED 5.0 oblong pedicle breaks just above calyx 5 9.0 oblate 9.0 very late University of Florida SCG_SCGWKM_R-Scategory Chandra et al. 2021 FRAP_FRAPTROM_TroloxEQumoles/g FW Capocasa et al. 2008 Università Politecnica delle Marche Strawberry ontology USDA PLOIDY_FCDAPIIBLM_x PLANTHABIT_PLHABM_1-9PLHABscale USDA 3.0 dead RosBREED PPMF_PPMFM_0-1PMFscale GDR FRFIRM_FRFIRMM_1-9FRFIRMscale GDR RosBREED very early 1 highly resistant/no symptoms 1 RUNNERDATE_FRUNNERM_date USDA GDR RosBREED RPF_RPF0-5M_0-5RESISTscale SPO_SPOM_1-9SUSCEPscale RosBREED GDR high 7 UPOV SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale 2 even GDR RosBREED STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale 2 calyx size equal to fruit diameter highly susceptible/severe symptoms 5 HD_FIRSTHARVM_date USDA USDA 5FRW_FRFRESHWT5LM_g USDA FLDATE100_FLDATE100M_FLDATE100date 2 semi-upright DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL USDA outwards 2 UPOV CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale RosBREED CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale GDR 6.0 rhombic slightly below 2 GDR RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale RosBREED UPOV FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale GDR RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale RosBREED GDR highly resistant/no symptoms 1 dark red 9.0 GDR RosBREED FRFLCOL_FRFLCOLM_1-9FRFLCOLscale RosBREED GDR FRFLCOLDEPTH_FRCOLDEPTHM_% long conic 2 GDR RosBREED SPF_SPFM_0-5SUSCEPscale USDA LFRW_FRFRESHWLGM_g ACHENEVIABLE_ACHVIAM_% GDR RosBREED 1 long conic 2 divides at 25% from crown FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale USDA 3 breakage is inconsistent 9 stamens taller than receptacle Capocasa et al. 2008 Università Politecnica delle Marche FRFIRM_PENEM_g 4 globose USDA FRLEN_FRLENM_mm UPOV TRUSSSIZ_TRUSSM_1-9scale RosBREED SKINGLOSS_SKINGLOSSM_1-9GLOSSscale GDR PLOIDY_FCDAPIICFM_x USDA 9 very light brown or green USDA FRSHAPE_FRSHAPEGGM_TXT RFOF_RFOF1-5M_1-5RESISTscale UC Davis Pincot, et al. EVERBERIN_EVER0-1M_0-1EVERscale USDA University of Delaware LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW Biswas et al. 2019 strongly below surface 1 divides right before fruit 5.0 2 slightly uneven USDA FRDEV_FRDEVM_days 9 fully reflexed USDA RMH_RMHM_1-5RMHscale Pinkerton & Finn GDR TRUSSSIZ_TRUSSCOUNTM_ave RosBREED 1 conic GDR FLEVAL_FLEVALM_0-1FLEVALscale RosBREED USDA RPF_RPF%M_% soft 1 GDR RosBREED SDE_SDEM_1-9SUSCEPscale UPOV BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale yellowish white 2 RNEOP_RNEOP0-6M_0-6RESISTscale Fernandez et al. University of North Carolina USDA FRpH_pH2M_pH FLDATE1_FLDATE1M_FLDATE1date USDA GDR ECOGDDREQ_GDDFM_days RosBREED level with surface 3 no fruit 1 BEARINGTYPE_BEARINGRBM_count GDR RosBREED 1 plant has no stamens RosBREED GDR SPOAP_SPOAPM_1-9SUSCEPscale CE_CROPEST_g USDA light orange 3 Zurn, et al. RFOF_RFOF%M_% USDA FLDATE2_FLDATE2M_date USDA slightly uneven 3 FLDIA_FLDIAM_cm UPOV strongly uneven 3 USDA DAU_RUNDAUM_count 1.0 very resistant, healthy USDA ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq RosBREED HARVDATE_HARVDATEM_date GDR 2 level with fruit SKINCOL_REFLECTCIXM_ChromaIndex Capocasa et al. 2008 Università Politecnica delle Marche GDR RosBREED ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale 3 small USDA TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq Vining, et al. RVD_RVD1-3M_1-3RESISTscale Oregon State GDR RosBREED DRIPLOSS_DRIPLOSSM_g divides 75% from crown 4 FRD_FRRIPEM_days USDA early 3 protruding 3 9 extremely vigorous RMaPh_ RMaPh%M_ % USDA Zurn, et al. 1 does not remove 1 even or very slightly uneven USDA RMYFR_RMYFRM_% FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale USDA USDA STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale Università Politecnica delle Marche Capocasa et al. 2008 TITA_TITAM_TITAMeqNAOH/100g TITA_TITA0.5M_TITAMeq/100gCA USDA resistant R STEMBREAK_PEDBRKM_text USDA 9 very easily removed 1 stamens shorter than receptacle slightly symptomatic 1.5 calyx attachment raised above shoulder (necked) 1 GDR RosBREED PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale medium orange 4 9 blackish red 8.0 large to very large ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale UPOV USDA RPO_RPOM_% 4 slightly above 5 medium number of flowers highly susceptible/severe symptoms 8 plant has stamens 9 3 low red 3 very large 9.0 GDR RosBREED FRFRESHWT_FRFRESHWT5M_g 1 divides close to crown USDA PLANTHT_PLANTHTM_cm very high 9.0 GDR RosBREED SSC_SSCM_SSCg/100g 10.0 blackish red 2 slightly below surface 1 no runners UC Davis RMaPh_ RMaPhM_1-5RESISTscale Knapp, et al. reniform 1 BLDATE1_FLDATE1M_FLDATE1date USDA 6.0 pink spreading 1 Pinkerton & Finn USDA RPP_RPPM_Rf NEM-RF_RPPM_Rf intermediate 5 complete fruit set 9 1 not uniform/malformed high yield 1 white 1 conic-wedge 3 RVD_RVD%_% USDA GDR ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale RosBREED 1 calyx size smaller than fruit diameter 9 hundreds USDA RTL_RUNTOTLENM_m greenish 1 USDA RPCRR_RPC%M_% inserted 1 3 raised USDA RPOAP_RPOAP%M_% USDA FLPETALNUM_FLPETALNUMM_average UPOV CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale globose 3 CPBR RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale 9 completely uniform/symmetrical and attractive dull 1 orange red 5.0 CPBR FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale strongly uneven 5 5.0 medium S susceptible few flowers 3 RosBREED GDR CALYXSZ_CALYXSZM_1-3CALYXSZscale calyx attachment is sunken below shoulder 5 NCRI RPCCR_RPCCR1-8M_1-8RESISTscale Eikemo, et al. GDR SMYFR_SMYFRM_1-9SUSCEPscale RosBREED conic 2 pinkish white 1 white 1 acronym RDE_REDM_1-9RESISTscale USDA not sweet, bad off-flavors 1 University of California, Davis Pincot et al. 2020 RVD_RVD1-9M_1-9RESISTScale 9 very firm highly susceptible/severe symptoms 9 2 yellow RUNNERNUM_RUNNERNUMCT_count USDA USDA RPCCR_RPCCR%M_% low density 3 2 partially remontant fully remontant 3 3 same level highly resistant/no symptoms 9 1 no fruit set low yield 3 large 7.0 Y Stem breaks just above calyx, good for fresh market FRYIELD_FRYIELD1-4M_1-4FRYIELDscale USDA very small to small 2 7 pedicle breaks well above calyx highly resistant/no symptoms 9 globose-conic 5.0 V variable, no consistent breaking habit cordate 3 Y** stem breaks well above calyx small to medium 4 9 dark red upright 1 UPOV FLDIA_FLDIAM_1-9FLDIAUPOVscale 1 high density 1 no flowers strongly below 1 4 ovate PEDICELLEN_PEDICELLENM_cm USDA 1 very soft DNARATIO_DNADAPIICFM_DNA/stdDNAILF USDA N stem does not break, berry separates from calyx easily overcropped 9 FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale GDR RosBREED few 3 not reflexed 1 very glossy 9 above surface 4 upwards 1 USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg 3 calyx attachment is even with fruit 2.5 very symptomatic 5.0 strongly above 5 globose-conic 5 medium 1 sunken 6.0 fingerling FRFLAV_FRFLAVM_1-9FRFLAVscale GDR RosBREED GDR RosBREED TotalPhenolics_F-CM_mg/L_GallicAcidEQ wedged 9.0 3 calyx size larger than fruit diameter divides 50% from crown 3 no fruit set 4 7 cordiform highly resistant/no symptoms 1 pedicle does not break 1 9 tough FRYIELD_FRYIELDTWM_g USDA 9 erect medium to large 6.0 1 dark even 1 9 very sweet, no off-flavors no fruit 1 medium yield 2 attribute 1 very small entity absent or very weak 1 Plant The plant resistance to red stele root rot caused by Phytophthora fragariae. Plant resistance to Phytophthora fragariae red stele root rot resistance to Phytophthora fragariae red stele root rot RPF PEDICLEBREAK Pedicle breakage relative to the calyx. breaking habit Pedicle breaking habit Pedicle SVD Plant Plant susceptibility to Verticillium dahliae wilt The plant susceptibility to verticillium wilt caused by Verticillium dahliae. susceptibility to Verticillium dahliae wilt 1-3 ACHENECOL UPOV scale BDF date first Bloom date first Julian date of first open flowers for an individual, whole plant, or population. Flower Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days flowering method Fruit development method Record the number of days from first flowering to ripe fruit. CALYXPLUG Calyx plug Presence or absence of central plug after calyx removal. Calyx plug 1-5 FRCOLUNI scale length The length of peduncle. Peduncle length Peduncle PEDUNCLEN fresh weight Fruit weight Fruit The weight of fresh fruit. 5FRW STAMENHGHT Stamen The height of the stamens relative to the receptacles. Stamen height height Largest fruit weight LFRW fresh weight largest Fruit The weight of fresh fruit. Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. Fruit Fruit titratable acidity TITA The titratable acidity content of fruit. titratable acidity Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. Calyx size method 1-9 RESIST scale Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Ferric reducing antioxidant power trolox method Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Flower diameter UPOV method 0-1 FLEVAL scale Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae % method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Observe and rate the height of the stamens relative to the receptacle at flower opening. Stamen height method Plant The plant resistance to powdery mildew caused by Podosphaera aphanis. RPOAP resistance to Podosphaera aphanis powdery mildew Plant resistance to Podosphaera aphanis powdery mildew The plant resistance to root knot nematode Meloidogyne hapla. Plant resistance to Meloidogyne hapla root knot nematode Plant RMH resistance to Meloidogyne hapla root knot nematode Resistance to Neopestalotiopsis sp. scale 0-6 Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants date Reproductive factor The total phenolic acids of fruit. Fruit Fruit total phenolic acids content TPHE total phenolic acids content Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Fruit pH 2 method 1-9 FLDIA UPOV scale Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Fruit firmness method Fruit FRFIRM Fruit flesh firmness flesh firmness The firmness of fruit flesh. Ferric reducing antioxidant power FSH method Plant resistance to necrosis caused by Neopestalotiopsis sp. resistance to necrosis caused by Neopestalotiopsis sp. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). The plant resistance to necrosis caused by Neopestalotiopsis sp. Plant RNEOP Harvest and record the largest fruit over the harvest season. Fruit fresh weight largest method Plant habit scale method Observe the plant and assess the erectness or spreading of the leaves along the ground. Flower diameter diameter Flower FLDIA The diameter of flower at fully open. Fruit uniformity of color Fruit uniformity of color Fruit uniformity of color. FRCOLORUNIF Plant The fruit yield of one individual. fruit yield Plant fruit yield FRYIELD The toughness of skin. Skin SKINTOUGH Skin toughness toughness Susceptibility to Colletotrichum gloeosporioides weekly method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-3 method 1-9 FRSHAPE UPOV scale Plant Plant resistance to Pratylenchus penetrans root lesion nematode The plant resistance to root lesion nematode Pratylenchus penetrans. resistance to Pratylenchus penetrans root lesion nematode RPP flower development Plant flower development Plant ECOGDDREQ Growing degree days to first flowering. Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Resistance to Mycosphaerella fragariae method The presence of powdery mildew on fruit. Fruit Fruit presence of powdery mildew presence of powdery mildew PPMF Depth of fruit flesh color Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. count FLDATE1 Flower date first Julian date of first open flowers for an individual, whole plant, or population. date first Flower % length Fruit length Fruit Length of fruit from calyx to tip. FRLEN Stem length of primary fruit from calyx to truss. Pedicle length length Pedicle PEDICELLEN Observe the plant and record the reflexion of the calyx relative to the fruit shoulders Calyx reflexion method Flower date last method Observe the plant and record the date when at the end of flowering. Total anthocyanins Cy-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Number of flowers per truss TRUSSSIZ Truss size size Truss meters Number of runners 1-9 method Observe and rate the number of runners per plant. x 1-9 CALYXREM scale total antioxidant content Leaf total antioxidant content Leaf Total antioxidant content of leaves. LFTOTANTIOX Estimate fruit yield and record it. Fruit yield 1-9 O method Fruit Harvest date harvest date HD Record the Julian date when first fruit ripens at a specific location. Fruit firmness penetrometer method Measure fruit firmness in grams using a hand-held penetrometer with a piston. 1-5 RESIST scale Observe and rate average fruit shape on 5 fruit per genotype. Fruit shape GG method Select 3 flower trusses, count the flowers and average the results. Number of flowers per truss count position CALYXPOS The position of the calyx attachment relative to the fruit shoulder. Calyx position Calyx Stamen Stamen presence presence The presence of stamens. STAMENPRES date first relative Flower date first relative Relative date of first open flowers for an individual, whole plant, or population. Flower FLDATE1REL Resistance to Macrophomina phaseolina method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. 1-5 TREXPOS UPOV scale Calyx position 1-3 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method Total anthocyanins Pg-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Fruit yield total weight method Weigh total fruit yield and record it. First runner date Runner RUNNERDATE first emergence The Julian date of appearance of the first runners. Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method Truss exposure 1-5 method Observe the truss location relative to the foliage canopy and record it. SDE susceptibility to Diplocarpon earliana Plant susceptibility to leaf scorch caused by Diplocarpon earliana Plant The plant susceptibility to leaf scorch caused by Diplocarpon earliana 1-4 ACHENEPOS UPOV scale The plant resistance to leaf blight caused by Phomopsis obscurans. resistance to Phomopsis obscurans leaf blight Plant resistance to Phomopsis obscurans leaf blight RPO Skin color chromameter reflect chroma index method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Indication of resistance to Botrytis The presence of grey mold caused by Botrytis cinerea on the plant or fruit. Plant BOTRYTIS presence of grey mold days 0-7 TRUSS scale Presence of powdery mildew on fruit method Observe the presence of powdery mildew on ripe fruit. Ease of calyx removal method Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Susceptibility to Podosphaera aphanis method Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. BEARINGTYPE bearing type Plant bearing type Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. Plant 1-9 STAMPRES scale 1-8 RESIST scale Measure the length of the fruit from the calyx to the tip and record in mm. Fruit length method Fruit ferric reducing antioxidant power ferric reducing antioxidant power Fruit Ferric reducing antioxidant power of fruit. FRAP Fruit shape UPOV method Observe and rate average fruit shape on 3-5 fruit per genotype. Skin color color SKINCOL Skin Skin color on the sun side of a fully ripe fruit. Fruit ripening trait Fruit ripening trait FRRP The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA FSH EQ μmoles/g fruit Record the Julian date when first fruit ripens at a specific location. harvest date HARVDATE Fruit harvest date Fruit Observe the number of fruit that a single plant sets in a specific location. Fruit set method Meq NaOH/100g Fruit Fruit development FRDEV Number of days from flowering to ripe fruit. development 1-3 CALYXSZ scale 1-9 PLANTVIGOR scale 1-9 FRSHAPUNI GRIN scale Flower date first method Observe the plant and record the date the first flowers open. Achene position 1-4 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Skin color chromameter reflect L* brightness method Plant vigor The vigor of plant. PLANTVIGOR vigor Plant Fruit total water soluble solid content SSC The soluble solids content of fruit. Fruit total water soluble solid content Plant resistance to Phytophthora cactorum root rot RPCRR Plant resistance to Phytophthora cactorum root rot The plant resistance to root rot caused by Phytophthora cactorum. DNA/std DNA_ILF FLDATE100 date FRFLCOL flesh color The color of fruit flesh. Fruit Fruit flesh color Plant habit PLANTHABIT habit Plant The degree of erectness of the leaves. Record the number of weeks of flowering after week 11 from the first week of bloom. Perpetual flowering RB method R-S category 0-1 STAMPRES scale Plant The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. SMYFR susceptibility to Mycosphaerella fragariae leaf spot Plant susceptibility to Mycosphaerella fragariae leaf spot Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. FLDATE1 Date FLEVAL The weekly development of flowers. Weekly flower evaluation development Flower Fruit yield 1-4 method Estimate fruit yield and record it. Fruit shape RB method Observe and rate average fruit shape on 3-5 fruit per genotype. STEMBREAK Pedicle Pedicle breakage relative to the calyx. breaking habit Stem breaking The number of runners. Runner number number RUNNERNUM Runner development Number of days from flowering to ripe fruit. FRD Fruit ripen days Fruit Julian date of peak flowering for an individual, whole plant, or population. Flower date 100% FLDATE100 date 100% Flower Achene color ACHENECOL The color of achene on the sun side of a fully ripe fruit. Achene color Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. Percentage of viable achenes method Truss exposure 0-1 method Observe the truss location relative to the foliage canopy and record it. Plant susceptibility to Phytophthora fragariae red stele root rot The plant susceptibility to red stele root rot caused by Phytophthora fragariae. SPF Plant susceptibility to Phytophthora fragariae red stele root rot GG scale Count the number of petals on 10 flowers and average the results Petal number average method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method average DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method 0-1 BOTRYITIS scale Luminance The flavor of fruit. Fruit flavor FRFLAV flavor Fruit The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides SCG Plant 1-9 FRSHAPE RB scale Plant resistance to Verticillium dahliae wilt RVD Plant resistance to Verticillium dahliae The plant resistance to verticillium wilt caused by Verticillium dahliae. Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Titratable acidity citric acid method FRpH Susceptibility to Phomopsis obscurans method Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Harvest date method Record the Julian date when first fruit ripens at a specific location. Meq/100g citric acid Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight 1 method Plant fruit development Growing degree days to first harvest. ECOGDDREQH fruit development Plant 0-5 SUSCEP scale LFPUBESC Leaf pubescence The density of trichomes on the leaf surface. Leaf pubescence size CALYXSZ Calyx size Calyx The size of the calyx. 1-9 FRGLOSS scale 1-5 PEDUNCLEN scale 1-3 pubescence scale DNA/std DNA_IBL Ease of calyx removal. Calyx ease of removal Calyx ease of removal CALYXREMOVE FRUITSET Fruit set Fruit The effeciency of fruit set at a specific location. set Number of runners count method Harvest and count the number of runners collected from a single crown on a single day in a specific location. Color uniformity method Observe fruit color uniformity from even or very slighly unevento strongly uneven. 1-9 SUSCEP scale Record the Julian date when first fruit develops ripe color on at least half of its surface First fruit color method 1-9 FRFLCOL scale Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Harvest all runners on a single day and measure the total length. Runner total length method Count the total number of daughter plants from a single plant harvested on a single day. Daughter plantlet count cm 1-9 FRYIELD scale Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Resistance to Meloidogyne hapla method Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Skin color 1-9 method Runner The total length of runners from a single plant harvested on a single day at a specific location. Runner total length RTL length total FLDATE2 date last Julian date of last flowering for an individual, whole plant, or population. Flower Flower date last The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. resistance to Colletotrichum acutatum anthracnose fruit rot Fruit resistance to Colletotrichum acutatum anthracnose fruit rot Fruit RAFR Presence of stamens 0-1 method Observe presence of anthers and record it. Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx plug method Measure the diameter of the flowers at fully open Flower diameter method Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Susceptibility to Verticillium dahliae 1-5 method Chroma Index Fruit drip loss drip loss Fruit DRIP LOSS Percent weight lost when frozen berries are thawed. Appearance of first color on fruit FRCOL1 Appearance of first color on fruit. first color appearance Fruit Resistance to Verticillium dahliae 1-3 method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. mg/L Pg-3-gluc kg−1 exposure Truss Truss exposure The position of the truss relative to the foliage. TREXPO Resistance to Phytophthora cactorum CR 1-5 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. 1-3 PLHAB scale Skin color 1-10 method Observe and rate the fruit skin color. Depth of fruit flesh color. Fruit flesh depth of color FRFLCOLDEPTH flesh depth of color Fruit Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR % method Fruit shape uniformity shape uniformity FRSHAPEUNIF Fruit shape uniformity. Fruit Resistance to Phytophthora fragariae 0-5 method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Resistance to Verticillium dahliae scale 1-9 SSC:TA method Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. 1-9 FRFIRM scale The weight of fresh fruit. Fruit fresh weight FRFRESHWT Fruit fresh weight Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae % method Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. Fruit Fruit total anthocyanin content ATCYN total anthocyanin content The total anthocyanins of fruit. SPOAP susceptibility to Podosphaera aphanis Plant Plant susceptibility to Podosphaera aphanis powdery mildew The plant susceptibility to powdery mildew caused by Podosphaera aphanis. Observe presence of anthers and record it. Presence of stamens 1-9 method Plant resistance to Macrophomina phaseolina charcoal rot Plant resistance to Macrophomina phaseolina charcoal rot RMaPh The plant resistance to charcoal rot caused by Macrophomina phaseolina. Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Fruit flesh color method 1-9 RUNNERNUM scale 1-9 SKINTOUGH scale 1-9 FRUITSET scale Flower date first relative method Observe the plant and record the relative flowering of the first open flowers. 0-1 TREXPOS scale The firmness of fruit flesh. Fruit FRFIRM Fruit flesh firmness flesh firmness Drip loss method Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. height PLANTHT Plant height Plant Height of tallest trifoliate leaf. Fruit pH method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. CE The amount of harvested fruit per plant per season. Plant fruit yield Crop estimate Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-5 method The glossiness of skin. SKINGLOSS Skin gloss Skin gloss Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Perpetual flowering method reflexion Reflexion of the calyx relative to the fruit shoulders. CALYXREFLX Calyx reflexion Calyx Flower date 100% method Observe the plant and record the date when at peak flowering. 1-5 RMH scale total anthocyanin content Fruit TACY Total anthocyanins The total anthocyanins of fruit. Plant resistance to Verticillium dahliae wilt Plant RVD The plant resistance to verticillium wilt caused by Verticillium dahliae. resistance to Verticillium dahliae wilt DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. DNA ration determination using DAPI with internal standard = iceberg lettuce method Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. Observe where the pedicle breaks during harvest. Pedicle breakage method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Observe the plant and record the Julian date the first runners emerge at a specific location. First runner date method 1-7 RUNNERNUM scale Measure the length of the pedicel of the primary fruit from the calyx to the truss. Pedicel measurement method Ratio of sample DNA to standard DNA. Plant DNA ratio Plant DNA ratio DNARATIO Weekly flowering evaluation Observe the plant weekly and record if it is flowering. Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-9 method Plant resistance to Diplocarpon earlianum leaf scorch resistance to Diplocarpon earlianum leaf scorch RDE The plant resistance to leaf scorch caused by Diplocarpon earliana. Plant 1-9 FRFLAV scale 1-9 SKINCOL scale Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Susceptibility to Diplocarpon earlianum method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. Susceptibility to Phytophthora fragariae method Observe the number of flowers per inflorescence. Number of flowers per truss estimation g/100g Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight method Plant bearing type Whether or not the plant has an everbearing habit EVERBERIN Everbearing ACHENEVIABLE The percentage of achenes that are viable. viable Achene Percentage of viable achenes Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-3 method resistance to Fusarium oxysporum f. sp. fragariae wilt Plant Plant resistance to Fusarium oxysporum f. sp. fragariae wilt RFOF The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Stembreak txt Soluble solids method Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina % method Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method Growing degree days harvest method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Plant height method Measure the tallest trifoliate leaf from crown to base of leaflets. mg/L Gallic Acid EQ Susceptibility to Mycosphaerella fragariae method Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. The titratable acidity content of fruit. titratable acidity Fruit titratable acidity Fruit TITA Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Crop estimate method grams Achene position The postion of achene relative to the fruit surface. ACHENEPOS Achene position Observe average peduncle length and record it. Peduncle length method mg/L Cy-3-gluc Eq Trolox EQ μmoles/g FW variable_of Plant susceptibility to Phomopsis obscurans leaf blight The plant susceptibility to leaf blight caused by Phomopsis obscurans. susceptibility to Phomopsis obscurans leaf blight Plant SPO Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. Fruit fresh weight 5L method 1-4 FRYIELD scale DAU Runner Runner daughters The total number of daughter plantlets per plant harvested on a single day at a specific location. daughters mm Observe plant vigor of the two clones and record the average. Plant vigor method Fruit yield 1-9 method Estimate fruit yield and record it. 1-9 FLDATE1REL UPOV scale method_of Resistance to Verticillium dahliae % method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. 0-1 CALPLUG scale Resistance to Diplocarpon earlianum method Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Fruit pH pH FRpH Fruit The pH of fruit. Observe and rate the number of runners per plant. Number of runners 1-7 method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis % method 1-9 FRYIELD Ordinal scale Fruit flavor method Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Folin-Ciocalteu method 0-1 EVER scale Observe fruit appearance from not uniform to completely uniform. Fruit appearance method Skin color on the sun side of a fully ripe fruit. color SKINCOL Skin Skin color 1-3 ACHENEPOS scale The shape of fruit. Fruit shape Fruit shape FRSHAPE 1-7 PEDBRK scale 1-3 CALYXPOS UPOV scale 1-3 RESIST scale Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Leaf total antioxidant assay method Measure the length of the epipodium of the primary fruit from the calyx to the truss. Epipodium measurement method 0-6 RESIST scale g 1-3 FRCOLUNI scale length EPILGT Epipodium Stem length of primary fruit from calyx to truss. Epipodium length Observe the presence of flowers during each week in July, August and September. Everbearing flowering method 1-9 STAMENHGHT scale 1-4 PERPFL UPOV scale Average flower petal number. Flower FLPETALNUM petal number Flower petal number Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Titratable acidity NaOH method 1-9 ACHENECOL scale scale_of Ratio Plant resistance to Mycosphaerella fragariae leaf spot The plant resistance to Mycosphaerella fragariae. resistance to Mycosphaerella fragariae leaf spot Plant RMYFR 1-3 CALYXREFLX UPOV scale Resistance to Colletotrichum acutatum anthracnose fruit rot % method Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. FLDATE2 date resistance to Phytophthora cactorum crown rot Plant resistance to Phytophthora cactorum crown rot RPCCR The plant resistance to crown rot caused by Phytophthora cactorum. Plant 1-10 SKINCOL UPOV scale A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. Titratable acidity 0.5 g citric acid method 1-9 PLHAB scale 0-1 PMF scale Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis 1-9 method 1-5 SUSCEP scale 1-9 CALYXREFLX scale ploidy Plant PLOIDY The number of sets of chromosomes in the nucleus. Plant ploidy 1-5 CALYXPOS scale