RPO_RPOM_% USDA USDA RMYFR_RMYFRM_% 7.0 light red FRFRESHWT_FRFRESHWT5M_g RosBREED GDR GDR RosBREED SMYFR_SMYFRM_1-9SUSCEPscale 3 breakage is inconsistent DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL USDA RMaPh_ RMaPh%M_ % USDA Zurn, et al. 1 dead GDR DRIPLOSS_DRIPLOSSM_g RosBREED STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale USDA 1 highly resistant/no symptoms LFPUBESC_LFPUBESCM_1-3LFPUBESCscale University of Delaware Biswas et al. 2019 1 absent or very weak RosBREED TRUSSSIZ_TRUSSCOUNTM_ave GDR EVERBERIN_EVER0-1M_0-1EVERscale USDA EPILGT_EPILGTM_cm USDA UPOV TRUSSSIZ_TRUSSM_1-9scale FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale USDA RosBREED ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq GDR RPOAP_RPOAP%M_% USDA 5FRW_FRFRESHWT5LM_g USDA RNEOP_RNEOP0-6M_0-6RESISTscale University of North Carolina Fernandez et al. USDA RTL_RUNTOTLENM_m USDA FRYIELD_FRYIELD1-4M_1-4FRYIELDscale small to medium 4 9 erect RosBREED GDR CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale UPOV FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale attribute HARVDATE_HARVDATEM_date GDR RosBREED GDR ECOGDDREQ_GDDFM_days RosBREED USDA FRRP_FRRPM_Ratio high density 1 1.5 slightly symptomatic FLDATE100_FLDATE100M_FLDATE100date USDA dead 3.0 1 even USDA FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale 3 few UPOV TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale SPF_SPFM_0-5SUSCEPscale GDR RosBREED RUNNERDATE_FRUNNERM_date USDA 9.0 wedged 5.0 orange red TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA RosBREED GDR SSC_SSCM_SSCg/100g Eikemo, et al. RPCCR_RPCCR1-8M_1-8RESISTscale NCRI USDA FRDEV_FRDEVM_days FRYIELD_FRYIELDTWM_g USDA very small 1 UPOV FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale many 7 5 medium 1 conic USDA RPCCR_RPCCR%M_% UPOV CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale Jiménez, et al. RPCCR_RPCCR1-5M_1-5RESISTscale UC Davis USDA RPOAP_RPOAP1-9M_1-9RESISTscale USDA NEM-RF_RPPM_Rf RPP_RPPM_Rf Pinkerton & Finn PLANTHT_PLANTHTM_cm USDA ovate 4 UPOV FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale Capocasa et al. 2008 FRAP_FRAPTROM_TroloxEQumoles/g FW Università Politecnica delle Marche Università Politecnica delle Marche FRAP_FRAPFSHM_FSHEQumoles/g fruit Capocasa et al. 2008 RosBREED CALYXSZ_CALYXSZM_1-3CALYXSZscale GDR RPF_RPF%M_% USDA FRFLAV_FRFLAVM_1-9FRFLAVscale RosBREED GDR medium number of flowers 5 4 globose 1 spreading DAU_RUNDAUM_count USDA 1 no runners RosBREED FRpH_pHM_pH GDR USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg RPCRR_RPC%M_% USDA 10.0 blackish red Chandra et al. 2021 University of Florida SCG_SCGWKM_R-Scategory USDA Pinkerton & Finn RMH_RMHM_1-5RMHscale 3 slightly uneven 3 globose 9 highly resistant/no symptoms day neutral 4 1 no fruit set Strawberry ontology level with fruit 2 RosBREED CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale GDR PLANTHABIT_PLHABM_1-9PLHABscale USDA completely uniform/symmetrical and attractive 9 protruding 3 3 raised RosBREED GDR FRFLCOL_FRFLCOLM_1-9FRFLCOLscale RosBREED GDR PPMF_PPMFM_0-1PMFscale 7 high Capocasa et al. 2008 Università Politecnica delle Marche SKINCOL_REFLECTCIXM_ChromaIndex oblong 5.0 PEDICELLEN_PEDICELLENM_cm USDA RosBREED GDR ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale USDA FRD_FRRIPEM_days USDA BLDATE1_FLDATE1M_FLDATE1date 1 not remontant RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale GDR SDE_SDEM_1-9SUSCEPscale RosBREED GDR GDR SKINCOL_SKINCOL1-9M_1-9SKINCOLscale RosBREED 3 low yield RosBREED SKINGLOSS_SKINGLOSSM_1-9GLOSSscale GDR BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale UPOV RFOF_RFOF1-5M_1-5RESISTscale Pincot, et al. UC Davis 4 above surface RUNNERNUM_RUNNERNUMCT_count USDA stamens taller than receptacle 9 medium density 2 5 medium USDA FRLEN_FRLENM_mm 2.5 very symptomatic very large 9.0 3 downwards USDA FRUITSET_FRSETM_1-9FRUITSETscale RosBREED ACHENEVIABLE_ACHVIAM_% GDR 9.0 very high FRCOL1_FRCOL1M_date USDA DNARATIO_DNADAPIICFM_DNA/stdDNAILF USDA very small to small 2 Università Politecnica delle Marche Capocasa et al. 2008 SKINCOL_REFLECTLM_L* 3 cordate RosBREED FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale GDR highly susceptible/severe symptoms 1 pedicle does not break 1 FLDATE2_FLDATE2M_date USDA 1 highly resistant/no symptoms 9 plant has stamens PLOIDY_FCDAPIIBLM_x USDA UC Davis Feldmann, et al. SVD_SVD1-5M_1-5SUSCEPscale RVD_RVD1-9M_1-9RESISTScale Pincot et al. 2020 University of California, Davis many flowers 7 2 slightly uneven USDA TITA_TITA0.5M_TITAMeq/100gCA low 3 1 dull USDA BOTRYTIS_RBCM_0-1BOTRYTISscale RosBREED GDR FRFLCOLDEPTH_FRCOLDEPTHM_% FLDIA_FLDIAM_1-9FLDIAUPOVscale UPOV RosBREED RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale GDR calyx attachment is even with fruit 3 Università Politecnica delle Marche TITA_TITAM_TITAMeqNAOH/100g Capocasa et al. 2008 2 slightly below surface 5.0 globose-conic 4 no fruit set RosBREED PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale GDR 9 very easily removed medium orange 4 FRSHAPE_FRSHAPEGGM_TXT USDA USDA CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale GDR FLEVAL_FLEVALM_0-1FLEVALscale RosBREED 5 globose-conic 7.0 oblate Biswas et al. 2019 LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW University of Delaware 1.0 very resistant, healthy 1 inserted ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA 5.0 strongly above 7 pedicle breaks well above calyx CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale UPOV STEMBREAK_PEDBRKM_text USDA RosBREED BEARINGTYPE_BEARINGRBM_count GDR SPO_SPOM_1-9SUSCEPscale RosBREED GDR USDA HD_FIRSTHARVM_date hundreds 9 strongly below 1 GDR ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale RosBREED RFOF_RFOF%M_% USDA Zurn, et al. LFRW_FRFRESHWLGM_g USDA 1 dark very glossy 9 RPF_RPF0-5M_0-5RESISTscale GDR RosBREED RosBREED TITA_TITAM_TITAMeq/100gCA GDR fully remontant 3 RosBREED GDR ECOGDDREQH_GDDHM_days 9 tough 1 upwards RosBREED GDR TREXPOS_TREXPOS0-1M_0-1TREXPOSscale 3 level with surface USDA FLDATE1_FLDATE1M_FLDATE1date USDA CE_CROPEST_g USDA FLPETALNUM_FLPETALNUMM_average 1 sunken reniform 1 3 conic-wedge ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale UPOV 9.0 oblate 9 very sweet, no off-flavors 1 long conic FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale CPBR RosBREED SPOAP_SPOAPM_1-9SUSCEPscale GDR RDE_REDM_1-9RESISTscale USDA FRFIRM_FRFIRMM_1-9FRFIRMscale RosBREED GDR FRpH_pH2M_pH USDA 1 no flowers USDA PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale GDR RosBREED STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale USDA PLOIDY_FCDAPIICFM_x GDR FRYIELD_FRYIELD1-9M_1-9FRYIELDscale RosBREED no fruit 1 Vining, et al. RVD_RVD1-3M_1-3RESISTscale Oregon State cordiform 7 divides at 25% from crown 2 8.0 medium red entity 5 highly susceptible/severe symptoms STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale USDA white 1 complete fruit set 9 medium 5.0 divides right before fruit 5.0 9 fully reflexed calyx size smaller than fruit diameter 1 3 small RosBREED CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale GDR highly susceptible/severe symptoms 1 TotalPhenolics_F-CM_mg/L_GallicAcidEQ GDR RosBREED dark red 9 CPBR PLANTHABIT_PLHABM_1-3PLHABscale 9 highly susceptible/severe symptoms UPOV FLDIA_FLDIAM_cm not uniform/malformed 1 stamens shorter than receptacle 1 very soft 1 fingerling 6.0 1 even or very slightly uneven 3 spreading 8.0 large to very large 5 calyx attachment is sunken below shoulder UC Davis RMaPh_ RMaPhM_1-5RESISTscale Knapp, et al. RVD_RVD%_% USDA large 7.0 long conic 2 4 divides 75% from crown 1 does not remove 9 blackish red conic 2 strongly below surface 1 Università Politecnica delle Marche Capocasa et al. 2008 FRFIRM_PENEM_g 9 extremely vigorous 1 white 1 greenish yellowish white 2 pedicle breaks just above calyx 5 stem does not break, berry separates from calyx easily N 8 highly susceptible/severe symptoms very late 9.0 calyx size equal to fruit diameter 2 intermediate 5 GDR PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale RosBREED FRRAFR_FRAFR%M_% University of Florida Salinas et al. 2019 even 2 1 calyx attachment raised above shoulder (necked) RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale CPBR 3 low density 1 pinkish white early 3 UPOV SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale 9 overcropped 6.0 pink divides 50% from crown 3 4 slightly above 1 highly resistant/no symptoms UPOV ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale 1 divides close to crown calyx size larger than fruit diameter 3 no fruit 1 9 very firm red 3 medium yield 2 3 strongly uneven 1 upright dark red 9.0 2 slightly below very light brown or green 9 8.0 circular variable, no consistent breaking habit V not reflexed 1 1 not sweet, bad off-flavors acronym late 7 medium to large 6.0 5 strongly uneven 2.0 mildly symptomatic susceptible S 2 outwards R resistant few flowers 3 Y** stem breaks well above calyx same level 3 soft 1 Y Stem breaks just above calyx, good for fresh market semi-upright 2 yellow 2 1 high yield partially remontant 2 light orange 3 plant has no stamens 1 1 very early rhombic 6.0 highly resistant/no symptoms 9 Crop estimate method Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. 1-7 RUNNERNUM scale Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method Fruit total anthocyanin content Fruit The total anthocyanins of fruit. total anthocyanin content ATCYN LFTOTANTIOX Leaf total antioxidant content Total antioxidant content of leaves. Leaf total antioxidant content Observe plant vigor of the two clones and record the average. Plant vigor method Calyx position 1-5 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. ripening trait Fruit Fruit ripening trait The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA FRRP Fruit set The effeciency of fruit set at a specific location. Fruit set FRUITSET The presence of grey mold caused by Botrytis cinerea on the plant or fruit. Plant presence of grey mold Indication of resistance to Botrytis BOTRYTIS Plant DNA ratio DNA ratio DNARATIO Plant Ratio of sample DNA to standard DNA. Fruit fresh weight Fruit The weight of fresh fruit. fresh weight FRFRESHWT Plant The degree of erectness of the leaves. PLANTHABIT Plant habit habit Fruit The color of fruit flesh. FRFLCOL flesh color Fruit flesh color 1-9 STAMPRES scale Susceptibility to Diplocarpon earlianum method Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Fruit yield 1-9 O method Estimate fruit yield and record it. 1-3 FRCOLUNI scale Titratable acidity citric acid method Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Presence of stamens 1-9 method Observe presence of anthers and record it. fruit yield Crop estimate CE The amount of harvested fruit per plant per season. Plant Skin color 1-10 method Observe and rate the fruit skin color. Meq/100g citric acid Record the Julian date when first fruit ripens at a specific location. Harvest date method Observe and rate average fruit shape on 5 fruit per genotype. Fruit shape GG method Truss exposure 1-5 method Observe the truss location relative to the foliage canopy and record it. Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Susceptibility to Colletotrichum gloeosporioides weekly method Fruit drip loss Percent weight lost when frozen berries are thawed. Fruit drip loss DRIP LOSS Fruit fresh weight 5L method Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. Observe and rate the number of runners per plant. Number of runners 1-9 method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Resistance to Macrophomina phaseolina method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. method_of 0-1 BOTRYITIS scale Observe the plant and assess the erectness or spreading of the leaves along the ground. Plant habit scale method 1-7 PEDBRK scale Number of runners 1-7 method Observe and rate the number of runners per plant. 1-9 FRSHAPE UPOV scale 1-9 FRSHAPE RB scale Plant fruit yield FRYIELD Plant fruit yield The fruit yield of one individual. Achene position 1-4 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Skin color chromameter reflect chroma index method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. The plant resistance to leaf scorch caused by Diplocarpon earliana. Plant resistance to Diplocarpon earlianum leaf scorch Plant resistance to Diplocarpon earlianum leaf scorch RDE Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Leaf total antioxidant assay method Fruit fresh weight method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. 1-9 CALYXREM scale grams 0-1 FLEVAL scale FLEVAL The weekly development of flowers. Weekly flower evaluation Flower development The length of peduncle. Peduncle PEDUNCLEN length Peduncle length Stamen presence presence STAMENPRES Stamen The presence of stamens. Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Fruit pH 2 method 1-3 CALYXPOS UPOV scale Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape UPOV method Fruit Fruit flavor FRFLAV flavor The flavor of fruit. SKINGLOSS gloss Skin gloss Skin The glossiness of skin. Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. Percentage of viable achenes method Observe average peduncle length and record it. Peduncle length method bearing type Plant Whether or not the plant has an everbearing habit EVERBERIN Everbearing Flower date first relative FLDATE1REL date first relative Relative date of first open flowers for an individual, whole plant, or population. Flower 1-9 FRGLOSS scale EPILGT Stem length of primary fruit from calyx to truss. Epipodium length length Epipodium Julian date of first open flowers for an individual, whole plant, or population. BDF Bloom date first date first Flower number Runner number RUNNERNUM Runner The number of runners. The position of the truss relative to the foliage. exposure TREXPO Truss Truss exposure SMYFR The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. Plant susceptibility to Mycosphaerella fragariae leaf spot susceptibility to Mycosphaerella fragariae leaf spot Plant FRCOLORUNIF Fruit Fruit uniformity of color. Fruit uniformity of color uniformity of color variable_of Calyx plug Presence or absence of central plug after calyx removal. CALYXPLUG Calyx plug FRFIRM The firmness of fruit flesh. flesh firmness Fruit flesh firmness Fruit days Fruit presence of powdery mildew presence of powdery mildew PPMF The presence of powdery mildew on fruit. Fruit The shape of fruit. FRSHAPE Fruit shape shape Fruit Fruit flesh depth of color Fruit flesh depth of color Depth of fruit flesh color. FRFLCOLDEPTH Fruit weight Fruit The weight of fresh fruit. 5FRW fresh weight Fruit appearance method Observe fruit appearance from not uniform to completely uniform. Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae % method Achene position ACHENEPOS position Achene The postion of achene relative to the fruit surface. Record the number of weeks of flowering after week 11 from the first week of bloom. Perpetual flowering RB method Fruit fresh weight 1 method Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Stamen height The height of the stamens relative to the receptacles. STAMENHGHT Stamen height SSC:TA method Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. Skin color 1-9 method Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. % Skin SKINCOL color Skin color Skin color on the sun side of a fully ripe fruit. Resistance to Phytophthora cactorum CR % method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. resistance to Podosphaera aphanis powdery mildew RPOAP Plant resistance to Podosphaera aphanis powdery mildew The plant resistance to powdery mildew caused by Podosphaera aphanis. Plant Estimate fruit yield and record it. Fruit yield 1-9 method Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Titratable acidity NaOH method date 100% Flower date 100% Flower Julian date of peak flowering for an individual, whole plant, or population. FLDATE100 RPO Plant resistance to Phomopsis obscurans leaf blight The plant resistance to leaf blight caused by Phomopsis obscurans. resistance to Phomopsis obscurans leaf blight 1-9 RUNNERNUM scale Runner total length method Harvest all runners on a single day and measure the total length. Fruit flesh color method Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina % method Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Ferric reducing antioxidant power trolox method Fruit titratable acidity titratable acidity Fruit The titratable acidity content of fruit. TITA FRpH FLDATE2 date Plant resistance to necrosis caused by Neopestalotiopsis sp. The plant resistance to necrosis caused by Neopestalotiopsis sp. Ferric reducing antioxidant power FSH method resistance to necrosis caused by Neopestalotiopsis sp. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). Plant RNEOP 1-4 FRYIELD scale susceptibility to Phomopsis obscurans leaf blight The plant susceptibility to leaf blight caused by Phomopsis obscurans. SPO Plant susceptibility to Phomopsis obscurans leaf blight Plant Susceptibility to Verticillium dahliae 1-5 method Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Fruit Total anthocyanins TACY total anthocyanin content The total anthocyanins of fruit. Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Pg-3-gluc method size TRUSSSIZ Truss Truss size Number of flowers per truss Growing degree days flowering method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Skin toughness Skin toughness SKINTOUGH The toughness of skin. Plant resistance to Macrophomina phaseolina charcoal rot The plant resistance to charcoal rot caused by Macrophomina phaseolina. RMaPh Plant resistance to Macrophomina phaseolina charcoal rot Runner total length Runner length total RTL The total length of runners from a single plant harvested on a single day at a specific location. Observe the number of fruit that a single plant sets in a specific location. Fruit set method 1-3 pubescence scale Measure the diameter of the flowers at fully open Flower diameter method 1-9 ACHENECOL scale Plant susceptibility to Podosphaera aphanis powdery mildew susceptibility to Podosphaera aphanis SPOAP Plant The plant susceptibility to powdery mildew caused by Podosphaera aphanis. Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae 1-3 method Presence of powdery mildew on fruit method Observe the presence of powdery mildew on ripe fruit. Observe the plant and record the reflexion of the calyx relative to the fruit shoulders Calyx reflexion method Skin color Skin color SKINCOL Skin color on the sun side of a fully ripe fruit. mg/L Gallic Acid EQ Achene color Achene The color of achene on the sun side of a fully ripe fruit. ACHENECOL color 0-5 SUSCEP scale g 1-5 SUSCEP scale ease of removal Calyx Ease of calyx removal. Calyx ease of removal CALYXREMOVE Daughter plantlet count Count the total number of daughter plants from a single plant harvested on a single day. Fruit development Number of days from flowering to ripe fruit. Fruit development FRDEV Meq NaOH/100g date HD Record the Julian date when first fruit ripens at a specific location. Harvest date Fruit harvest date Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx plug method Observe the plant and record the Julian date the first runners emerge at a specific location. First runner date method Observe the presence of grey mold on the plant or fruit. Resistance to Botrytis cinerea method The plant susceptibility to red stele root rot caused by Phytophthora fragariae. Plant susceptibility to Phytophthora fragariae red stele root rot Plant susceptibility to Phytophthora fragariae red stele root rot SPF Fruit titratable acidity titratable acidity The titratable acidity content of fruit. TITA Fruit 1-9 FLDIA UPOV scale Observe the plant and record the date when at the end of flowering. Flower date last method LFPUBESC The density of trichomes on the leaf surface. Leaf pubescence pubescence Leaf PLOIDY ploidy Plant The number of sets of chromosomes in the nucleus. Plant ploidy Observe the plant weekly and record if it is flowering. Weekly flowering evaluation petal number Average flower petal number. Flower FLPETALNUM Flower petal number resistance to Colletotrichum acutatum anthracnose fruit rot Fruit resistance to Colletotrichum acutatum anthracnose fruit rot Fruit RAFR The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape RB method Observe and rate the height of the stamens relative to the receptacle at flower opening. Stamen height method RPP Plant resistance to Pratylenchus penetrans root lesion nematode resistance to Pratylenchus penetrans root lesion nematode Plant The plant resistance to root lesion nematode Pratylenchus penetrans. Plant Plant resistance to Meloidogyne hapla root knot nematode resistance to Meloidogyne hapla root knot nematode The plant resistance to root knot nematode Meloidogyne hapla. RMH Resistance to Phytophthora fragariae % method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. 1-10 SKINCOL UPOV scale 1-5 RESIST scale Count the number of petals on 10 flowers and average the results Petal number average method Resistance to Podosphaera aphanis % method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. 1-5 PEDUNCLEN scale Calyx reflexion CALYXREFLX Calyx Reflexion of the calyx relative to the fruit shoulders. reflexion Luminance Percentage of viable achenes viable The percentage of achenes that are viable. Achene ACHENEVIABLE Resistance to Fusarium oxysporum f. sp. fragariae % method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. RPCCR Plant resistance to Phytophthora cactorum crown rot resistance to Phytophthora cactorum crown rot The plant resistance to crown rot caused by Phytophthora cactorum. Plant 1-5 CALYXPOS scale Weigh total fruit yield and record it. Fruit yield total weight method Pedicle length PEDICELLEN Pedicle Stem length of primary fruit from calyx to truss. length The soluble solids content of fruit. Fruit Fruit total water soluble solid content SSC total water soluble solid content Resistance to Meloidogyne hapla method Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Observe where the pedicle breaks during harvest. Pedicle breakage method Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Fruit firmness method Resistance to Diplocarpon earlianum method Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. fruit development ECOGDDREQH Plant fruit development Growing degree days to first harvest. Plant FLDIA The diameter of flower at fully open. diameter Flower diameter Flower Plant Plant resistance to Phytophthora fragariae red stele root rot resistance to Phytophthora fragariae red stele root rot RPF The plant resistance to red stele root rot caused by Phytophthora fragariae. The size of the calyx. CALYXSZ Calyx Calyx size size 1-9 SKINCOL scale Plant height method Measure the tallest trifoliate leaf from crown to base of leaflets. Fruit yield 1-4 method Estimate fruit yield and record it. Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae 0-5 method 1-8 RESIST scale Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. 1-4 PERPFL UPOV scale Perpetual flowering method Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Susceptibility to Mycosphaerella fragariae method 0-1 STAMPRES scale RFOF Plant resistance to Fusarium oxysporum f. sp. fragariae wilt resistance to Fusarium oxysporum f. sp. fragariae wilt Plant The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Fruit flavor method Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Truss exposure 0-1 method Observe the truss location relative to the foliage canopy and record it. Titratable acidity 0.5 g citric acid method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. 0-7 TRUSS scale first color appearance Fruit FRCOL1 Appearance of first color on fruit. Appearance of first color on fruit FSH EQ μmoles/g fruit Resistance to Colletotrichum acutatum anthracnose fruit rot % method Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. cm 1-3 ACHENECOL UPOV scale susceptibility to Verticillium dahliae wilt SVD Plant The plant susceptibility to verticillium wilt caused by Verticillium dahliae. Plant susceptibility to Verticillium dahliae wilt Stem breaking Pedicle STEMBREAK breaking habit Pedicle breakage relative to the calyx. Soluble solids method Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. 1-9 PLANTVIGOR scale 1-9 STAMENHGHT scale Resistance to Podosphaera aphanis 1-9 method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Reproductive factor Observe the number of flowers per inflorescence. Number of flowers per truss estimation height Plant height Plant PLANTHT Height of tallest trifoliate leaf. fresh weight largest Fruit The weight of fresh fruit. LFRW Largest fruit weight 1-5 TREXPOS UPOV scale The plant susceptibility to leaf scorch caused by Diplocarpon earliana SDE Plant susceptibility to Diplocarpon earliana Plant susceptibility to leaf scorch caused by Diplocarpon earliana Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. 1-9 FRFIRM scale The firmness of fruit flesh. FRFIRM flesh firmness Fruit flesh firmness Fruit The Julian date of appearance of the first runners. Runner first emergence RUNNERDATE First runner date 1-9 PLHAB scale Growing degree days to first flowering. Plant flower development flower development Plant ECOGDDREQ Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-3 method Drip loss method Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. 1-3 CALYXSZ scale Presence of stamens 0-1 method Observe presence of anthers and record it. Depth of fruit flesh color Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. vigor Plant Plant vigor PLANTVIGOR The vigor of plant. ferric reducing antioxidant power Ferric reducing antioxidant power of fruit. Fruit ferric reducing antioxidant power Fruit FRAP 1-9 FRYIELD scale Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. Susceptibility to Phytophthora fragariae method Susceptibility to Podosphaera aphanis method Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. total phenolic acids content Fruit total phenolic acids content Fruit TPHE The total phenolic acids of fruit. count Fruit length method Measure the length of the fruit from the calyx to the tip and record in mm. average mg/L Pg-3-gluc kg−1 1-9 RESIST scale Trolox EQ μmoles/g FW 1-3 ACHENEPOS scale susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides SCG The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. Plant Calyx position CALYXPOS The position of the calyx attachment relative to the fruit shoulder. position Calyx Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Cy-3-gluc method Folin-Ciocalteu method Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Susceptibility to Phomopsis obscurans method Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Flower diameter UPOV method Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. The plant resistance to verticillium wilt caused by Verticillium dahliae. resistance to Verticillium dahliae RVD Plant resistance to Verticillium dahliae wilt Plant 1-4 ACHENEPOS UPOV scale 1-3 CALYXREFLX UPOV scale g/100g Julian date of first open flowers for an individual, whole plant, or population. date first FLDATE1 Flower Flower date first meters 1-9 FRSHAPUNI GRIN scale Fruit shape uniformity shape uniformity FRSHAPEUNIF Fruit Fruit shape uniformity. Color uniformity method Observe fruit color uniformity from even or very slighly unevento strongly uneven. date last Julian date of last flowering for an individual, whole plant, or population. Flower date last FLDATE2 Flower Resistance to Mycosphaerella fragariae method Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. mg/L Cy-3-gluc Eq Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. Fruit pH method Plant resistance to Mycosphaerella fragariae leaf spot The plant resistance to Mycosphaerella fragariae. Plant resistance to Mycosphaerella fragariae leaf spot RMYFR 0-1 CALPLUG scale Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. 0-1 TREXPOS scale Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-3 method Ratio Measure the length of the pedicel of the primary fruit from the calyx to the truss. Pedicel measurement method R-S category 0-1 EVER scale FRpH The pH of fruit. Fruit Fruit pH pH 0-1 PMF scale 1-5 RMH scale Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Number of runners count method Harvest and count the number of runners collected from a single crown on a single day in a specific location. The plant resistance to verticillium wilt caused by Verticillium dahliae. RVD Plant resistance to Verticillium dahliae wilt Plant resistance to Verticillium dahliae wilt 1-9 FRYIELD Ordinal scale Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-9 method Fruit Fruit harvest date harvest date HARVDATE Record the Julian date when first fruit ripens at a specific location. Observe the plant and record the date the first flowers open. Flower date first method 1-5 FRCOLUNI scale 1-9 SKINTOUGH scale The total number of daughter plantlets per plant harvested on a single day at a specific location. Runner Runner daughters daughters DAU 1-9 CALYXREFLX scale Everbearing flowering method Observe the presence of flowers during each week in July, August and September. DNA/std DNA_IBL Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-3 method 1-3 RESIST scale Observe the plant and record the relative flowering of the first open flowers. Flower date first relative method scale_of Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR 1-8 method Resistance to Phytophthora cactorum CR 1-5 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. GG scale 0-6 RESIST scale FLDATE1 Date DNA/std DNA_ILF mm DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. DNA ration determination using DAPI with internal standard = iceberg lettuce method Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. Plant resistance to Phytophthora cactorum root rot RPCRR Plant The plant resistance to root rot caused by Phytophthora cactorum. resistance to Phytophthora cactorum root rot Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. BEARINGTYPE Plant bearing type bearing type Plant 1-9 SUSCEP scale Ease of calyx removal method Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Stembreak txt Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Leaf pubescence image capturing method 1-9 FRFLCOL scale FRLEN length Fruit length Fruit Length of fruit from calyx to tip. Number of days from flowering to ripe fruit. Fruit Fruit ripen days development FRD Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days harvest method Resistance to Verticillium dahliae scale 1-9 Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Harvest and record the largest fruit over the harvest season. Fruit fresh weight largest method Resistance to Neopestalotiopsis sp. scale 0-6 Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants 1-3 PLHAB scale Epipodium measurement method Measure the length of the epipodium of the primary fruit from the calyx to the truss. Pedicle breaking habit PEDICLEBREAK breaking habit Pedicle Pedicle breakage relative to the calyx. x Skin color chromameter reflect L* brightness method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Chroma Index FLDATE100 date DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. 1-9 FLDATE1REL UPOV scale Measure fruit firmness in grams using a hand-held penetrometer with a piston. Fruit firmness penetrometer method Flower date 100% method Observe the plant and record the date when at peak flowering. Calyx size method Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. 1-9 FRUITSET scale Record the number of days from first flowering to ripe fruit. Fruit development method First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface 1-9 FRFLAV scale