FLDATE2_FLDATE2M_date USDA SPOAP_SPOAPM_1-9SUSCEPscale GDR RosBREED 5FRW_FRFRESHWT5LM_g USDA DAU_RUNDAUM_count USDA CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale GDR RosBREED GDR RosBREED TotalPhenolics_F-CM_mg/L_GallicAcidEQ HD_FIRSTHARVM_date USDA SKINCOL_SKINCOL1-9M_1-9SKINCOLscale GDR RosBREED Capocasa et al. 2008 Università Politecnica delle Marche FRAP_FRAPTROM_TroloxEQumoles/g FW Zurn, et al. USDA RMaPh_ RMaPh%M_ % RosBREED GDR SPF_SPFM_0-5SUSCEPscale 6.0 pink CPBR RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale RUNNERDATE_FRUNNERM_date USDA 1 strongly below RosBREED ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale GDR Università Politecnica delle Marche Capocasa et al. 2008 FRAP_FRAPFSHM_FSHEQumoles/g fruit FRYIELD_FRYIELD1-4M_1-4FRYIELDscale USDA USDA RPCRR_RPC%M_% Capocasa et al. 2008 Università Politecnica delle Marche TITA_TITAM_TITAMeqNAOH/100g RosBREED FRYIELD_FRYIELD1-9M_1-9FRYIELDscale GDR PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale RosBREED GDR ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale RosBREED GDR RPP_RPPM_Rf NEM-RF_RPPM_Rf USDA Pinkerton & Finn PLOIDY_FCDAPIICFM_x USDA Vining, et al. Oregon State RVD_RVD1-3M_1-3RESISTscale 2 slightly below highly susceptible/severe symptoms 1 Capocasa et al. 2008 Università Politecnica delle Marche SKINCOL_REFLECTLM_L* blackish red 9 Jiménez, et al. UC Davis RPCCR_RPCCR1-5M_1-5RESISTscale 5 medium low yield 3 breakage is inconsistent 3 CPBR FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale Pincot, et al. UC Davis RFOF_RFOF1-5M_1-5RESISTscale intermediate 5 USDA FRD_FRRIPEM_days 3 few flowers GDR ECOGDDREQH_GDDHM_days RosBREED 5 medium number of flowers 3 fully remontant 3 conic-wedge PLANTHT_PLANTHTM_cm USDA GDR RosBREED SDE_SDEM_1-9SUSCEPscale 1 high yield DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL USDA USDA CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale 9.0 very late USDA RVD_RVD%_% 4 ovate FRUITSET_FRSETM_1-9FRUITSETscale USDA FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale UPOV UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale USDA GDR RosBREED FLEVAL_FLEVALM_0-1FLEVALscale RosBREED GDR FRFLAV_FRFLAVM_1-9FRFLAVscale RosBREED GDR CALYXSZ_CALYXSZM_1-3CALYXSZscale 9 very firm R resistant small to medium 4 highly susceptible/severe symptoms 8 GDR RosBREED CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale many 7 3 low density USDA RPCCR_RPCCR%M_% RPOAP_RPOAP1-9M_1-9RESISTscale USDA RosBREED GDR FRFLCOLDEPTH_FRCOLDEPTHM_% USDA ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA DNARATIO_DNADAPIICFM_DNA/stdDNAILF RMYFR_RMYFRM_% USDA RosBREED GDR TITA_TITAM_TITAMeq/100gCA SMYFR_SMYFRM_1-9SUSCEPscale RosBREED GDR FLDATE100_FLDATE100M_FLDATE100date USDA USDA FLPETALNUM_FLPETALNUMM_average PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale USDA UPOV BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale sunken 1 RFOF_RFOF%M_% Zurn, et al. USDA reniform 1 1 no flowers USDA RDE_REDM_1-9RESISTscale 7.0 oblate fully reflexed 9 highly susceptible/severe symptoms 9 FRCOL1_FRCOL1M_date USDA 3 slightly uneven GDR RosBREED ECOGDDREQ_GDDFM_days University of Delaware LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW Biswas et al. 2019 GDR RosBREED PPMF_PPMFM_0-1PMFscale medium 5.0 9 plant has stamens RPCCR_RPCCR1-8M_1-8RESISTscale NCRI Eikemo, et al. USDA LFRW_FRFRESHWLGM_g USDA EPILGT_EPILGTM_cm 2 yellowish white RosBREED GDR CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale highly resistant/no symptoms 1 FRLEN_FRLENM_mm USDA CPBR PLANTHABIT_PLHABM_1-3PLHABscale 1 very soft 4 medium orange PLANTHABIT_PLHABM_1-9PLHABscale USDA high density 1 HARVDATE_HARVDATEM_date GDR RosBREED USDA EVERBERIN_EVER0-1M_0-1EVERscale oblate 9.0 divides right before fruit 5.0 3 strongly uneven RosBREED GDR RPF_RPF0-5M_0-5RESISTscale ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale USDA 1 not sweet, bad off-flavors calyx size smaller than fruit diameter 1 3 calyx attachment is even with fruit GDR RosBREED ACHENEVIABLE_ACHVIAM_% University of Delaware LFPUBESC_LFPUBESCM_1-3LFPUBESCscale Biswas et al. 2019 GDR RosBREED FRFLCOL_FRFLCOLM_1-9FRFLCOLscale 5.0 orange red very small 1 white 1 upright 1 Università Politecnica delle Marche SKINCOL_REFLECTCIXM_ChromaIndex Capocasa et al. 2008 RPO_RPOM_% USDA RosBREED GDR FRFIRM_FRFIRMM_1-9FRFIRMscale 1 no runners light orange 3 Salinas et al. 2019 University of Florida FRRAFR_FRAFR%M_% conic 1 USDA FRYIELD_FRYIELDTWM_g 9 very sweet, no off-flavors SCG_SCGWKM_R-Scategory Chandra et al. 2021 University of Florida USDA FRpH_pH2M_pH cordate 3 semi-upright 2 even 1 red 3 USDA FRRP_FRRPM_Ratio globose 4 3 protruding USDA STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale UPOV 4 day neutral SSC_SSCM_SSCg/100g RosBREED GDR wedged 9.0 GDR RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale very glossy 9 GDR RosBREED DRIPLOSS_DRIPLOSSM_g stem does not break, berry separates from calyx easily N RTL_RUNTOTLENM_m USDA strongly above 5.0 1 no fruit GDR RosBREED SPO_SPOM_1-9SUSCEPscale stamens shorter than receptacle 1 UPOV FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale USDA FLDATE1_FLDATE1M_FLDATE1date USDA Pinkerton & Finn RMH_RMHM_1-5RMHscale 2 yellow blackish red 10.0 SVD_SVD1-5M_1-5SUSCEPscale UC Davis Feldmann, et al. CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale UPOV medium 5 1 pedicle does not break TITA_TITA0.5M_TITAMeq/100gCA USDA RosBREED GDR FRpH_pHM_pH USDA RPF_RPF%M_% 3 divides 50% from crown SKINGLOSS_SKINGLOSSM_1-9GLOSSscale GDR RosBREED RosBREED FRFRESHWT_FRFRESHWT5M_g GDR RMaPh_ RMaPhM_1-5RESISTscale UC Davis Knapp, et al. slightly above 4 3 downwards RosBREED GDR STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale mildly symptomatic 2.0 highly resistant/no symptoms 9 UPOV CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale USDA RPOAP_RPOAP%M_% BOTRYTIS_RBCM_0-1BOTRYTISscale USDA 9.0 very large 1 spreading USDA BLDATE1_FLDATE1M_FLDATE1date 9 stamens taller than receptacle calyx attachment raised above shoulder (necked) 1 UPOV SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale not reflexed 1 7 late FLDIA_FLDIAM_cm UPOV globose-conic 5 3 small 1 strongly below surface 3 low 5 highly susceptible/severe symptoms GDR RosBREED FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale 1 upwards even or very slightly uneven 1 3.0 dead 7.0 light red 1 highly susceptible/severe symptoms Strawberry ontology divides close to crown 1 1 very early FRDEV_FRDEVM_days USDA 9 very easily removed UPOV FLDIA_FLDIAM_1-9FLDIAUPOVscale 2 outwards 1 absent or very weak 1 plant has no stamens 9 very light brown or green TRUSSSIZ_TRUSSM_1-9scale UPOV USDA FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale 7 cordiform same level 3 2 even 9 dark red FRFIRM_PENEM_g Capocasa et al. 2008 Università Politecnica delle Marche greenish 1 GDR RosBREED ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq 9 erect many flowers 7 5 pedicle breaks just above calyx 1 long conic very high 9.0 conic 2 USDA TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq 1 no fruit slightly below surface 2 UPOV FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale University of North Carolina Fernandez et al. RNEOP_RNEOP0-6M_0-6RESISTscale FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg USDA 1 not uniform/malformed TRUSSSIZ_TRUSSCOUNTM_ave GDR RosBREED 3 calyx size larger than fruit diameter extremely vigorous 9 7 pedicle breaks well above calyx 6.0 rhombic divides at 25% from crown 2 RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale GDR RosBREED 2 partially remontant stem breaks well above calyx Y** PLOIDY_FCDAPIIBLM_x USDA calyx size equal to fruit diameter 2 USDA PEDICELLEN_PEDICELLENM_cm TREXPOS_TREXPOS0-1M_0-1TREXPOSscale RosBREED GDR 4 no fruit set 9 complete fruit set oblong 5.0 attribute 2 slightly uneven long conic 2 very small to small 2 8.0 large to very large USDA CE_CROPEST_g very symptomatic 2.5 RVD_RVD1-9M_1-9RESISTScale University of California, Davis Pincot et al. 2020 acronym 9 highly resistant/no symptoms susceptible S 1 not remontant 8.0 circular Stem breaks just above calyx, good for fresh market Y 1 inserted V variable, no consistent breaking habit pinkish white 1 USDA FRSHAPE_FRSHAPEGGM_TXT hundreds 9 medium yield 2 USDA RUNNERNUM_RUNNERNUMCT_count GDR RosBREED PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale does not remove 1 fingerling 6.0 RosBREED GDR BEARINGTYPE_BEARINGRBM_count large 7.0 USDA STEMBREAK_PEDBRKM_text dull 1 overcropped 9 globose-conic 5.0 divides 75% from crown 4 dark red 9.0 early 3 level with surface 3 4 above surface 9 completely uniform/symmetrical and attractive 1.5 slightly symptomatic globose 3 few 3 soft 1 1 no fruit set 1 dead 3 spreading 5 strongly uneven highly resistant/no symptoms 1 8.0 medium red 7 high 2 medium density 1.0 very resistant, healthy 1 dark 5 calyx attachment is sunken below shoulder 9 tough level with fruit 2 entity 1 white raised 3 medium to large 6.0 1 highly resistant/no symptoms The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. resistance to Fusarium oxysporum f. sp. fragariae wilt RFOF Plant Plant resistance to Fusarium oxysporum f. sp. fragariae wilt Measure the length of the epipodium of the primary fruit from the calyx to the truss. Epipodium measurement method Observe the plant and record the relative flowering of the first open flowers. Flower date first relative method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Cy-3-gluc method Calyx reflexion method Observe the plant and record the reflexion of the calyx relative to the fruit shoulders 0-1 EVER scale date first Bloom date first Julian date of first open flowers for an individual, whole plant, or population. BDF Flower The plant resistance to root lesion nematode Pratylenchus penetrans. resistance to Pratylenchus penetrans root lesion nematode Plant RPP Plant resistance to Pratylenchus penetrans root lesion nematode 1-9 FLDATE1REL UPOV scale Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Fruit pH 2 method Calyx reflexion reflexion Reflexion of the calyx relative to the fruit shoulders. CALYXREFLX Calyx Calyx size Calyx size CALYXSZ The size of the calyx. mg/L Pg-3-gluc kg−1 average 1-5 PEDUNCLEN scale PEDICELLEN Pedicle length length Stem length of primary fruit from calyx to truss. Pedicle Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Drip loss method Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Fruit firmness method Achene position 1-4 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. SCG Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant SPO Plant susceptibility to Phomopsis obscurans leaf blight susceptibility to Phomopsis obscurans leaf blight Plant The plant susceptibility to leaf blight caused by Phomopsis obscurans. Peduncle length method Observe average peduncle length and record it. mm 1-9 RESIST scale 5FRW Fruit weight Fruit The weight of fresh fruit. fresh weight Fruit fresh weight method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit flavor method Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Relative date of first open flowers for an individual, whole plant, or population. Flower date first relative date first relative FLDATE1REL Flower Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days flowering method First runner date method Observe the plant and record the Julian date the first runners emerge at a specific location. Fruit uniformity of color Fruit uniformity of color. Fruit FRCOLORUNIF uniformity of color Resistance to Phomopsis obscurans method Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. 1-3 ACHENEPOS scale 1-3 pubescence scale Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Perpetual flowering method first emergence The Julian date of appearance of the first runners. First runner date Runner RUNNERDATE FLDIA The diameter of flower at fully open. diameter Flower Flower diameter harvest date Fruit harvest date Fruit Record the Julian date when first fruit ripens at a specific location. HARVDATE Plant Ratio of sample DNA to standard DNA. DNA ratio DNARATIO Plant DNA ratio The total anthocyanins of fruit. Fruit total anthocyanin content TACY Total anthocyanins Susceptibility to Podosphaera aphanis method Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. Petal number average method Count the number of petals on 10 flowers and average the results Fruit shape uniformity. FRSHAPEUNIF Fruit shape uniformity shape uniformity Fruit Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Susceptibility to Mycosphaerella fragariae method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-5 method Percentage of viable achenes Achene ACHENEVIABLE The percentage of achenes that are viable. viable Fruit yield total weight method Weigh total fruit yield and record it. Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Estimate fruit yield and record it. Fruit yield 1-9 method Daughter plantlet count Count the total number of daughter plants from a single plant harvested on a single day. Fruit length method Measure the length of the fruit from the calyx to the tip and record in mm. Flower Weekly flower evaluation The weekly development of flowers. development FLEVAL Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape UPOV method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum RR% method Indication of resistance to Botrytis Plant presence of grey mold The presence of grey mold caused by Botrytis cinerea on the plant or fruit. BOTRYTIS 0-6 RESIST scale FRFIRM The firmness of fruit flesh. Fruit Fruit flesh firmness flesh firmness Calyx CALYXPOS Calyx position position The position of the calyx attachment relative to the fruit shoulder. Flower Julian date of first open flowers for an individual, whole plant, or population. FLDATE1 date first Flower date first Observe fruit appearance from not uniform to completely uniform. Fruit appearance method FRFLAV Fruit flavor Fruit flavor The flavor of fruit. development Number of days from flowering to ripe fruit. FRD Fruit Fruit ripen days mg/L Gallic Acid EQ SKINTOUGH Skin toughness Skin toughness The toughness of skin. Fruit drip loss DRIP LOSS Fruit drip loss Percent weight lost when frozen berries are thawed. Fruit set set FRUITSET Fruit The effeciency of fruit set at a specific location. Observe the plant and record the date the first flowers open. Flower date first method Stamen height method Observe and rate the height of the stamens relative to the receptacle at flower opening. Harvest date method Record the Julian date when first fruit ripens at a specific location. days number RUNNERNUM Runner number Runner The number of runners. FLDATE100 date 1-9 FRGLOSS scale Fruit set method Observe the number of fruit that a single plant sets in a specific location. Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. Calyx size method Susceptibility to Diplocarpon earlianum method Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Susceptibility to Phomopsis obscurans method Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. grams The plant resistance to Mycosphaerella fragariae. resistance to Mycosphaerella fragariae leaf spot RMYFR Plant Plant resistance to Mycosphaerella fragariae leaf spot Titratable acidity NaOH method Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR 1-5 method Ferric reducing antioxidant power of fruit. Fruit ferric reducing antioxidant power FRAP Fruit ferric reducing antioxidant power resistance to Phytophthora cactorum root rot Plant Plant resistance to Phytophthora cactorum root rot RPCRR The plant resistance to root rot caused by Phytophthora cactorum. Plant Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. Plant bearing type BEARINGTYPE bearing type Meq NaOH/100g Fruit TITA Fruit titratable acidity The titratable acidity content of fruit. titratable acidity 1-9 PLANTVIGOR scale 1-9 CALYXREM scale 1-9 FRFLCOL scale g RMH resistance to Meloidogyne hapla root knot nematode Plant resistance to Meloidogyne hapla root knot nematode The plant resistance to root knot nematode Meloidogyne hapla. Plant Perpetual flowering RB method Record the number of weeks of flowering after week 11 from the first week of bloom. DNA/std DNA_IBL Achene color 1-3 method Observe the average achene color on 3-5 fruit per genotype and record it. 1-9 SKINTOUGH scale presence of powdery mildew The presence of powdery mildew on fruit. Fruit Fruit presence of powdery mildew PPMF FLDATE1 Date STEMBREAK Stem breaking Pedicle breakage relative to the calyx. Pedicle breaking habit Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. Percentage of viable achenes method Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. 1-9 FRUITSET scale 0-1 PMF scale Calyx position 1-3 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae % method 1-8 RESIST scale Skin Skin color on the sun side of a fully ripe fruit. SKINCOL Skin color color Plant susceptibility to leaf scorch caused by Diplocarpon earliana susceptibility to Diplocarpon earliana Plant SDE The plant susceptibility to leaf scorch caused by Diplocarpon earliana Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Resistance to Diplocarpon earlianum method Observe the plant and assess the erectness or spreading of the leaves along the ground. Plant habit scale method susceptibility to Phytophthora fragariae red stele root rot Plant susceptibility to Phytophthora fragariae red stele root rot Plant SPF The plant susceptibility to red stele root rot caused by Phytophthora fragariae. Fruit shape GG method Observe and rate average fruit shape on 5 fruit per genotype. Select 3 flower trusses, count the flowers and average the results. Number of flowers per truss count Weekly flowering evaluation Observe the plant weekly and record if it is flowering. Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR % method 0-1 TREXPOS scale TPHE Fruit The total phenolic acids of fruit. total phenolic acids content Fruit total phenolic acids content Resistance to Phytophthora fragariae % method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Number of runners 1-9 method Observe and rate the number of runners per plant. Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Susceptibility to Colletotrichum gloeosporioides weekly method fruit yield Crop estimate The amount of harvested fruit per plant per season. CE Plant Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. The color of achene on the sun side of a fully ripe fruit. color Achene ACHENECOL Achene color Observe the number of flowers per inflorescence. Number of flowers per truss estimation Plant fruit development Plant fruit development ECOGDDREQH Growing degree days to first harvest. Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. FLPETALNUM Flower petal number Flower petal number Average flower petal number. 0-1 CALPLUG scale cm The position of the truss relative to the foliage. exposure TREXPO Truss Truss exposure Luminance Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina method Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method PEDICLEBREAK Pedicle breakage relative to the calyx. Pedicle breaking habit Pedicle breaking habit resistance to Diplocarpon earlianum leaf scorch The plant resistance to leaf scorch caused by Diplocarpon earliana. Plant resistance to Diplocarpon earlianum leaf scorch Plant RDE Harvest all runners on a single day and measure the total length. Runner total length method meters flesh depth of color Fruit flesh depth of color FRFLCOLDEPTH Fruit Depth of fruit flesh color. The plant resistance to verticillium wilt caused by Verticillium dahliae. Plant resistance to Verticillium dahliae wilt resistance to Verticillium dahliae Plant RVD Fruit FRCOL1 Appearance of first color on fruit. Appearance of first color on fruit first color appearance Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. resistance to Phytophthora cactorum crown rot The plant resistance to crown rot caused by Phytophthora cactorum. RPCCR Plant resistance to Phytophthora cactorum crown rot Plant count x 1-9 SKINCOL scale The glossiness of skin. SKINGLOSS Skin gloss Skin gloss harvest date Record the Julian date when first fruit ripens at a specific location. Fruit HD Harvest date Skin Skin color on the sun side of a fully ripe fruit. SKINCOL color Skin color 1-4 ACHENEPOS UPOV scale Calyx plug method Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. RTL Runner total length length total Runner The total length of runners from a single plant harvested on a single day at a specific location. FLDATE100 Flower Flower date 100% date 100% Julian date of peak flowering for an individual, whole plant, or population. Observe presence of anthers and record it. Presence of stamens 0-1 method Plant resistance to necrosis caused by Neopestalotiopsis sp. The plant resistance to necrosis caused by Neopestalotiopsis sp. RNEOP Ferric reducing antioxidant power FSH method resistance to necrosis caused by Neopestalotiopsis sp. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). Plant Truss exposure 1-5 method Observe the truss location relative to the foliage canopy and record it. Titratable acidity 0.5 g citric acid method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. LFRW Fruit fresh weight largest Largest fruit weight The weight of fresh fruit. Plant vigor method Observe plant vigor of the two clones and record the average. DNA/std DNA_ILF resistance to Colletotrichum acutatum anthracnose fruit rot Fruit The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. RAFR Fruit resistance to Colletotrichum acutatum anthracnose fruit rot Fruit fresh weight 5L method Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface Fruit pH The pH of fruit. Fruit FRpH pH SSC The soluble solids content of fruit. Fruit total water soluble solid content Fruit total water soluble solid content Stembreak txt Resistance to Pratylenchus penetrans number of nematodes/g root method Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. 1-7 PEDBRK scale SMYFR Plant susceptibility to Mycosphaerella fragariae leaf spot Plant susceptibility to Mycosphaerella fragariae leaf spot The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. % 1-5 SUSCEP scale Ease of calyx removal. ease of removal Calyx CALYXREMOVE Calyx ease of removal Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Folin-Ciocalteu method date last Julian date of last flowering for an individual, whole plant, or population. Flower FLDATE2 Flower date last Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. 1-5 RESIST scale Observe fruit color uniformity from even or very slighly unevento strongly uneven. Color uniformity method Plant height method Measure the tallest trifoliate leaf from crown to base of leaflets. 1-9 ACHENECOL scale Meq/100g citric acid 1-9 FLDIA UPOV scale DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. 0-1 FLEVAL scale 1-9 FRYIELD Ordinal scale 1-9 RUNNERNUM scale Observe the presence of flowers during each week in July, August and September. Everbearing flowering method 1-3 CALYXPOS UPOV scale Flower date 100% method Observe the plant and record the date when at peak flowering. total antioxidant content LFTOTANTIOX Leaf Total antioxidant content of leaves. Leaf total antioxidant content 0-1 STAMPRES scale 1-5 TREXPOS UPOV scale PLANTHABIT Plant The degree of erectness of the leaves. habit Plant habit 0-1 BOTRYITIS scale Measure the diameter of the flowers at fully open Flower diameter method resistance to Macrophomina phaseolina charcoal rot RMaPh Plant Plant resistance to Macrophomina phaseolina charcoal rot The plant resistance to charcoal rot caused by Macrophomina phaseolina. Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Skin color chromameter reflect L* brightness method 1-9 STAMENHGHT scale Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae 1-3 method DAU daughters Runner The total number of daughter plantlets per plant harvested on a single day at a specific location. Runner daughters Fruit shape Fruit shape FRSHAPE The shape of fruit. pubescence Leaf The density of trichomes on the leaf surface. LFPUBESC Leaf pubescence length The length of peduncle. Peduncle length Peduncle PEDUNCLEN Fruit flesh color method Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Resistance to Podosphaera aphanis % method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. The number of sets of chromosomes in the nucleus. Plant ploidy Plant ploidy PLOIDY The postion of achene relative to the fruit surface. ACHENEPOS Achene Achene position position Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Resistance to Meloidogyne hapla method RPF Plant resistance to Phytophthora fragariae red stele root rot Plant resistance to Phytophthora fragariae red stele root rot The plant resistance to red stele root rot caused by Phytophthora fragariae. DNA ration determination using DAPI with internal standard = iceberg lettuce method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis 1-9 method variable_of method_of 1-3 FRCOLUNI scale Fruit Fruit flesh color flesh color The color of fruit flesh. FRFLCOL 1-9 FRFIRM scale GG scale Fruit FRDEV Fruit development Number of days from flowering to ripe fruit. development total anthocyanin content ATCYN Fruit The total anthocyanins of fruit. Fruit total anthocyanin content Estimate fruit yield and record it. Fruit yield 1-9 O method Pedicle breakage method Observe where the pedicle breaks during harvest. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Ferric reducing antioxidant power trolox method Presence of powdery mildew on fruit method Observe the presence of powdery mildew on ripe fruit. 1-4 PERPFL UPOV scale Skin color 1-10 method Observe and rate the fruit skin color. Susceptibility to Phytophthora fragariae method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. Number of runners 1-7 method Observe and rate the number of runners per plant. 1-4 FRYIELD scale ECOGDDREQ flower development Plant flower development Growing degree days to first flowering. Plant Plant resistance to Podosphaera aphanis powdery mildew RPOAP resistance to Podosphaera aphanis powdery mildew Plant The plant resistance to powdery mildew caused by Podosphaera aphanis. Ratio Trolox EQ μmoles/g FW Fruit yield 1-4 method Estimate fruit yield and record it. Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Pg-3-gluc method 1-3 CALYXREFLX UPOV scale Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Resistance to Mycosphaerella fragariae method Pedicel measurement method Measure the length of the pedicel of the primary fruit from the calyx to the truss. 1-7 RUNNERNUM scale scale_of 1-9 CALYXREFLX scale Resistance to Verticillium dahliae scale 1-9 Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. Fruit pH method Plant fruit yield The fruit yield of one individual. Plant fruit yield FRYIELD 1-9 STAMPRES scale mg/L Cy-3-gluc Eq length Epipodium length EPILGT Epipodium Stem length of primary fruit from calyx to truss. Fruit fresh weight 1 method Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. 0-7 TRUSS scale Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Flower diameter UPOV method Plant Plant height Height of tallest trifoliate leaf. PLANTHT height The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA Fruit FRRP ripening trait Fruit ripening trait Susceptibility to Verticillium dahliae 1-5 method Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days harvest method Calyx Calyx plug Presence or absence of central plug after calyx removal. CALYXPLUG plug Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. Depth of fruit flesh color Observe the plant and record the date when at the end of flowering. Flower date last method 1-3 CALYXSZ scale Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-9 method R-S category Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants Resistance to Neopestalotiopsis sp. scale 0-6 EVERBERIN Whether or not the plant has an everbearing habit Plant bearing type Everbearing Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae 0-5 method Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape RB method 1-9 FRSHAPE UPOV scale The firmness of fruit flesh. Fruit FRFIRM flesh firmness Fruit flesh firmness Length of fruit from calyx to tip. length Fruit length FRLEN Fruit date Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae % method Observe the truss location relative to the foliage canopy and record it. Truss exposure 0-1 method 0-5 SUSCEP scale Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina % method Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Titratable acidity citric acid method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Chroma Index Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-3 method Plant resistance to Phomopsis obscurans leaf blight The plant resistance to leaf blight caused by Phomopsis obscurans. resistance to Phomopsis obscurans leaf blight RPO Fruit fresh weight fresh weight The weight of fresh fruit. FRFRESHWT Fruit 1-5 FRCOLUNI scale susceptibility to Podosphaera aphanis The plant susceptibility to powdery mildew caused by Podosphaera aphanis. SPOAP Plant Plant susceptibility to Podosphaera aphanis powdery mildew Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Ease of calyx removal method FRpH Reproductive factor 1-3 RESIST scale The titratable acidity content of fruit. titratable acidity Fruit TITA Fruit titratable acidity 1-9 FRFLAV scale 1-9 SUSCEP scale 1-9 FRSHAPE RB scale 1-3 ACHENECOL UPOV scale FLDATE2 date Stamen height The height of the stamens relative to the receptacles. height Stamen STAMENHGHT 1-9 FRSHAPUNI GRIN scale 1-9 FRYIELD scale Measure fruit firmness in grams using a hand-held penetrometer with a piston. Fruit firmness penetrometer method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Skin color chromameter reflect chroma index method 1-5 CALYXPOS scale Fruit development method Record the number of days from first flowering to ripe fruit. susceptibility to Verticillium dahliae wilt The plant susceptibility to verticillium wilt caused by Verticillium dahliae. Plant SVD Plant susceptibility to Verticillium dahliae wilt presence The presence of stamens. Stamen presence STAMENPRES Stamen Resistance to Colletotrichum acutatum anthracnose fruit rot % method Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. 1-10 SKINCOL UPOV scale 1-5 RMH scale FSH EQ μmoles/g fruit 1-9 PLHAB scale Plant PLANTVIGOR vigor The vigor of plant. Plant vigor Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. SSC:TA method Skin color 1-9 method Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Observe presence of anthers and record it. Presence of stamens 1-9 method Soluble solids method Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. RVD resistance to Verticillium dahliae wilt Plant Plant resistance to Verticillium dahliae wilt The plant resistance to verticillium wilt caused by Verticillium dahliae. 1-3 PLHAB scale Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Crop estimate method g/100g Harvest and record the largest fruit over the harvest season. Fruit fresh weight largest method Harvest and count the number of runners collected from a single crown on a single day in a specific location. Number of runners count method Truss size Number of flowers per truss TRUSSSIZ size Truss Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Leaf total antioxidant assay method