high yield 1 5.0 globose-conic 1 not remontant Capocasa et al. 2008 Università Politecnica delle Marche FRAP_FRAPTROM_TroloxEQumoles/g FW USDA RPOAP_RPOAP%M_% SKINCOL_REFLECTCIXM_ChromaIndex Capocasa et al. 2008 Università Politecnica delle Marche PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale RosBREED GDR conic 1 USDA RMaPh_ RMaPh%M_ % Zurn, et al. PEDICELLEN_PEDICELLENM_cm USDA pedicle breaks just above calyx 5 CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale USDA UC Davis SVD_SVD1-5M_1-5SUSCEPscale Feldmann, et al. 1 greenish FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale UPOV 1 upright Knapp, et al. UC Davis RMaPh_ RMaPhM_1-5RESISTscale GDR FRFLAV_FRFLAVM_1-9FRFLAVscale RosBREED inserted 1 UPOV CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale UPOV SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale FRFLCOL_FRFLCOLM_1-9FRFLCOLscale RosBREED GDR DAU_RUNDAUM_count USDA RVD_RVD1-3M_1-3RESISTscale Vining, et al. Oregon State SKINCOL_REFLECTLM_L* Capocasa et al. 2008 Università Politecnica delle Marche ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale UPOV very late 9.0 PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale USDA Fernandez et al. University of North Carolina RNEOP_RNEOP0-6M_0-6RESISTscale 9 tough spreading 3 Eikemo, et al. RPCCR_RPCCR1-8M_1-8RESISTscale NCRI 5FRW_FRFRESHWT5LM_g USDA Capocasa et al. 2008 Università Politecnica delle Marche TITA_TITAM_TITAMeqNAOH/100g RPP_RPPM_Rf USDA NEM-RF_RPPM_Rf Pinkerton & Finn highly susceptible/severe symptoms 1 very firm 9 UPOV FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale USDA RUNNERNUM_RUNNERNUMCT_count USDA FLDATE2_FLDATE2M_date PLANTHABIT_PLHABM_1-9PLHABscale USDA DNARATIO_DNADAPIICFM_DNA/stdDNAILF USDA very large 9.0 1 pinkish white circular 8.0 RosBREED FRFIRM_FRFIRMM_1-9FRFIRMscale GDR Pincot, et al. UC Davis RFOF_RFOF1-5M_1-5RESISTscale 1 not reflexed GDR FRFRESHWT_FRFRESHWT5M_g RosBREED GDR RosBREED CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale RosBREED TITA_TITAM_TITAMeq/100gCA GDR highly susceptible/severe symptoms 5 GDR RosBREED TRUSSSIZ_TRUSSCOUNTM_ave 2 outwards RosBREED GDR FLEVAL_FLEVALM_0-1FLEVALscale strongly uneven 5 FRpH_pHM_pH RosBREED GDR USDA RPF_RPF%M_% plant has no stamens 1 globose 4 FRFIRM_PENEM_g Università Politecnica delle Marche Capocasa et al. 2008 RPCRR_RPC%M_% USDA FRpH_pH2M_pH USDA TITA_TITA0.5M_TITAMeq/100gCA USDA RosBREED BEARINGTYPE_BEARINGRBM_count GDR day neutral 4 1.5 slightly symptomatic dark red 9 SKINCOL_SKINCOL1-9M_1-9SKINCOLscale RosBREED GDR 1 very soft FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale USDA 1 highly resistant/no symptoms RPOAP_RPOAP1-9M_1-9RESISTscale USDA fully reflexed 9 RosBREED PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale GDR few 3 attribute ECOGDDREQH_GDDHM_days RosBREED GDR FRLEN_FRLENM_mm USDA 9 extremely vigorous USDA FRYIELD_FRYIELD1-4M_1-4FRYIELDscale 5 calyx attachment is sunken below shoulder RosBREED SKINGLOSS_SKINGLOSSM_1-9GLOSSscale GDR RosBREED GDR FRYIELD_FRYIELD1-9M_1-9FRYIELDscale USDA FRDEV_FRDEVM_days GDR CALYXSZ_CALYXSZM_1-3CALYXSZscale RosBREED very early 1 USDA BOTRYTIS_RBCM_0-1BOTRYTISscale 7 many flowers 3 few flowers stem does not break, berry separates from calyx easily N partially remontant 2 GDR CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale RosBREED very symptomatic 2.5 very light brown or green 9 CPBR RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale SPF_SPFM_0-5SUSCEPscale RosBREED GDR EVERBERIN_EVER0-1M_0-1EVERscale USDA FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale RosBREED GDR 1 strongly below USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg 9 highly resistant/no symptoms RosBREED SMYFR_SMYFRM_1-9SUSCEPscale GDR 9 erect USDA FRCOL1_FRCOL1M_date USDA RUNNERDATE_FRUNNERM_date STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale RosBREED GDR highly susceptible/severe symptoms 8 RosBREED GDR SSC_SSCM_SSCg/100g 3 raised USDA RPO_RPOM_% no fruit 1 TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA large 7.0 STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale USDA USDA RMH_RMHM_1-5RMHscale Pinkerton & Finn above surface 4 BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale UPOV blackish red 10.0 spreading 1 GDR SPO_SPOM_1-9SUSCEPscale RosBREED FRSHAPE_FRSHAPEGGM_TXT USDA USDA EPILGT_EPILGTM_cm SCG_SCGWKM_R-Scategory Chandra et al. 2021 University of Florida GDR RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale ACHENEVIABLE_ACHVIAM_% RosBREED GDR USDA FRD_FRRIPEM_days 4 no fruit set 9.0 dark red RDE_REDM_1-9RESISTscale USDA RosBREED GDR TREXPOS_TREXPOS0-1M_0-1TREXPOSscale RosBREED ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale GDR overcropped 9 RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale GDR RosBREED medium density 2 USDA RFOF_RFOF%M_% Zurn, et al. 1 white UPOV TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale LFRW_FRFRESHWLGM_g USDA FRRP_FRRPM_Ratio USDA globose 3 UPOV FLDIA_FLDIAM_cm ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV RosBREED TotalPhenolics_F-CM_mg/L_GallicAcidEQ GDR slightly uneven 3 medium yield 2 RTL_RUNTOTLENM_m USDA 9 very sweet, no off-flavors USDA FRYIELD_FRYIELDTWM_g 2 slightly below surface 9 stamens taller than receptacle divides 50% from crown 3 semi-upright 2 RosBREED GDR ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq globose-conic 5 GDR RosBREED ECOGDDREQ_GDDFM_days sunken 1 5.0 medium RPF_RPF0-5M_0-5RESISTscale RosBREED GDR 2 level with fruit 9 very glossy RMYFR_RMYFRM_% USDA USDA STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale USDA ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq PLANTHT_PLANTHTM_cm USDA DRIPLOSS_DRIPLOSSM_g RosBREED GDR TRUSSSIZ_TRUSSM_1-9scale UPOV LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW Biswas et al. 2019 University of Delaware RosBREED GDR SPOAP_SPOAPM_1-9SUSCEPscale FLDATE100_FLDATE100M_FLDATE100date USDA CE_CROPEST_g USDA 3 early USDA FRUITSET_FRSETM_1-9FRUITSETscale FRRAFR_FRAFR%M_% University of Florida Salinas et al. 2019 hundreds 9 RosBREED GDR CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale 5.0 oblong mildly symptomatic 2.0 USDA FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale USDA FLPETALNUM_FLPETALNUMM_average FLDIA_FLDIAM_1-9FLDIAUPOVscale UPOV not uniform/malformed 1 oblate 9.0 1 reniform RosBREED SDE_SDEM_1-9SUSCEPscale GDR 6.0 medium to large LFPUBESC_LFPUBESCM_1-3LFPUBESCscale Biswas et al. 2019 University of Delaware same level 3 not sweet, bad off-flavors 1 3 breakage is inconsistent calyx size equal to fruit diameter 2 GDR ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale RosBREED variable, no consistent breaking habit V low 3 RosBREED HARVDATE_HARVDATEM_date GDR even 2 CPBR FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale late 7 small 3 2 long conic blackish red 9 9 complete fruit set susceptible S 1 dull 9 plant has stamens small to medium 4 pink 6.0 USDA BLDATE1_FLDATE1M_FLDATE1date UPOV FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale long conic 1 GDR PPMF_PPMFM_0-1PMFscale RosBREED USDA PLOIDY_FCDAPIIBLM_x 9 highly resistant/no symptoms 3 conic-wedge RosBREED FRFLCOLDEPTH_FRCOLDEPTHM_% GDR calyx attachment is even with fruit 3 pedicle breaks well above calyx 7 red 3 upwards 1 3 calyx size larger than fruit diameter RPCCR_RPCCR%M_% USDA Pincot et al. 2020 RVD_RVD1-9M_1-9RESISTScale University of California, Davis Jiménez, et al. RPCCR_RPCCR1-5M_1-5RESISTscale UC Davis completely uniform/symmetrical and attractive 9 no runners 1 slightly uneven 2 USDA RVD_RVD%_% 1 highly resistant/no symptoms 3 level with surface Strawberry ontology 9 very easily removed stamens shorter than receptacle 1 Università Politecnica delle Marche FRAP_FRAPFSHM_FSHEQumoles/g fruit Capocasa et al. 2008 dead 3.0 3 low yield 1 absent or very weak cordiform 7 USDA FLDATE1_FLDATE1M_FLDATE1date 5.0 divides right before fruit fingerling 6.0 large to very large 8.0 1 high density 8.0 medium red 1 dead CPBR PLANTHABIT_PLHABM_1-3PLHABscale calyx size smaller than fruit diameter 1 USDA DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL PLOIDY_FCDAPIICFM_x USDA protruding 3 CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale UPOV 1 no fruit set very resistant, healthy 1.0 many 7 4 slightly above even 1 1 divides close to crown medium number of flowers 5 2 divides at 25% from crown STEMBREAK_PEDBRKM_text USDA 1 does not remove 1 highly susceptible/severe symptoms 5 medium 5.0 orange red 4 ovate HD_FIRSTHARVM_date USDA conic 2 1 even or very slightly uneven 1 no fruit 4 medium orange 1 highly resistant/no symptoms medium 5 oblate 7.0 7 high entity strongly uneven 3 dark 1 yellow 2 highly susceptible/severe symptoms 9 strongly above 5.0 1 very small low density 3 3 downwards soft 1 R resistant 9.0 wedged white 1 fully remontant 3 9.0 very high light red 7.0 slightly below 2 2 very small to small 1 strongly below surface yellowish white 2 calyx attachment raised above shoulder (necked) 1 acronym 1 pedicle does not break 1 no flowers intermediate 5 cordate 3 Stem breaks just above calyx, good for fresh market Y 3 light orange 6.0 rhombic Y** stem breaks well above calyx divides 75% from crown 4 Peduncle length method Observe average peduncle length and record it. Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. Flower diameter method Measure the diameter of the flowers at fully open The plant susceptibility to leaf scorch caused by Diplocarpon earliana Plant susceptibility to leaf scorch caused by Diplocarpon earliana SDE susceptibility to Diplocarpon earliana Plant Resistance to Phytophthora cactorum CR 1-5 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. HARVDATE harvest date Fruit Fruit harvest date Record the Julian date when first fruit ripens at a specific location. Calyx Calyx reflexion reflexion Reflexion of the calyx relative to the fruit shoulders. CALYXREFLX The number of sets of chromosomes in the nucleus. Plant ploidy PLOIDY Plant ploidy SSC:TA method Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. R-S category Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Resistance to Diplocarpon earlianum method Plant vigor method Observe plant vigor of the two clones and record the average. days Julian date of first open flowers for an individual, whole plant, or population. date first Bloom date first BDF Flower The weight of fresh fruit. fresh weight 5FRW Fruit weight Fruit 1-5 CALYXPOS scale Fruit flesh firmness The firmness of fruit flesh. FRFIRM flesh firmness Fruit resistance to Verticillium dahliae Plant The plant resistance to verticillium wilt caused by Verticillium dahliae. RVD Plant resistance to Verticillium dahliae wilt TACY Total anthocyanins Fruit total anthocyanin content The total anthocyanins of fruit. Trolox EQ μmoles/g FW Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. 1-9 CALYXREFLX scale g 1-9 FRSHAPE RB scale Skin color color Skin Skin color on the sun side of a fully ripe fruit. SKINCOL The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA Fruit ripening trait FRRP Fruit ripening trait development FRD Number of days from flowering to ripe fruit. Fruit ripen days Fruit 1-9 FRFLAV scale BEARINGTYPE Plant bearing type Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. Plant bearing type Susceptibility to Phomopsis obscurans method Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Everbearing Whether or not the plant has an everbearing habit EVERBERIN Plant bearing type FRCOL1 first color appearance Appearance of first color on fruit Fruit Appearance of first color on fruit. The density of trichomes on the leaf surface. Leaf pubescence Leaf LFPUBESC pubescence 1-9 RUNNERNUM scale Plant DNA ratio DNARATIO Plant DNA ratio Ratio of sample DNA to standard DNA. 0-1 TREXPOS scale SKINCOL Skin Skin color color Skin color on the sun side of a fully ripe fruit. Calyx size The size of the calyx. CALYXSZ size Calyx Fruit FRCOLORUNIF uniformity of color Fruit uniformity of color Fruit uniformity of color. Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. Susceptibility to Podosphaera aphanis method 1-9 SUSCEP scale 1-5 FRCOLUNI scale Estimate fruit yield and record it. Fruit yield 1-9 O method RMaPh The plant resistance to charcoal rot caused by Macrophomina phaseolina. resistance to Macrophomina phaseolina charcoal rot Plant Plant resistance to Macrophomina phaseolina charcoal rot Susceptibility to Colletotrichum gloeosporioides weekly method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Fruit FRSHAPE Fruit shape The shape of fruit. shape % Fruit titratable acidity TITA The titratable acidity content of fruit. titratable acidity Fruit Plant Plant susceptibility to Phytophthora fragariae red stele root rot The plant susceptibility to red stele root rot caused by Phytophthora fragariae. SPF susceptibility to Phytophthora fragariae red stele root rot Fruit presence of powdery mildew presence of powdery mildew The presence of powdery mildew on fruit. Fruit PPMF 1-8 RESIST scale Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-3 method diameter FLDIA Flower diameter The diameter of flower at fully open. Flower ATCYN The total anthocyanins of fruit. Fruit total anthocyanin content total anthocyanin content Fruit Resistance to Phomopsis obscurans method Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. FRDEV Number of days from flowering to ripe fruit. Fruit development development Fruit Depth of fruit flesh color. FRFLCOLDEPTH Fruit flesh depth of color Fruit flesh depth of color 1-3 CALYXPOS UPOV scale Ratio Harvest and count the number of runners collected from a single crown on a single day in a specific location. Number of runners count method Epipodium length Stem length of primary fruit from calyx to truss. EPILGT Epipodium length 0-6 RESIST scale Plant resistance to Podosphaera aphanis powdery mildew Plant resistance to Podosphaera aphanis powdery mildew The plant resistance to powdery mildew caused by Podosphaera aphanis. RPOAP Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Ferric reducing antioxidant power trolox method Truss size Truss size TRUSSSIZ Number of flowers per truss Calyx position 1-5 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. FLDATE100 date flesh color FRFLCOL Fruit Fruit flesh color The color of fruit flesh. 1-9 CALYXREM scale Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. Calyx size method The presence of grey mold caused by Botrytis cinerea on the plant or fruit. BOTRYTIS Indication of resistance to Botrytis Plant presence of grey mold Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Ease of calyx removal method Calyx CALYXREMOVE Ease of calyx removal. ease of removal Calyx ease of removal Fruit fresh weight method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. 1-4 FRYIELD scale Plant habit Plant The degree of erectness of the leaves. habit PLANTHABIT Achene color 1-3 method Observe the average achene color on 3-5 fruit per genotype and record it. 1-9 FRSHAPE UPOV scale Peduncle length The length of peduncle. length PEDUNCLEN Peduncle First runner date method Observe the plant and record the Julian date the first runners emerge at a specific location. Total anthocyanins Cy-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Crop estimate method Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Presence of powdery mildew on fruit method Observe the presence of powdery mildew on ripe fruit. Achene color 1-9 method Observe the average achene color on 3-5 fruit per genotype and record it. RFOF The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. resistance to Fusarium oxysporum f. sp. fragariae wilt Plant resistance to Fusarium oxysporum f. sp. fragariae wilt Plant FSH EQ μmoles/g fruit DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method titratable acidity TITA Fruit The titratable acidity content of fruit. Fruit titratable acidity Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Titratable acidity NaOH method STAMENHGHT Stamen height The height of the stamens relative to the receptacles. height Stamen 1-3 PLHAB scale petal number Flower Flower petal number Average flower petal number. FLPETALNUM Achene color The color of achene on the sun side of a fully ripe fruit. color ACHENECOL Achene Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-4 method Observe fruit appearance from not uniform to completely uniform. Fruit appearance method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days harvest method The Julian date of appearance of the first runners. First runner date RUNNERDATE first emergence Runner Depth of fruit flesh color Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. Percentage of viable achenes method Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. plug Presence or absence of central plug after calyx removal. Calyx plug Calyx CALYXPLUG resistance to Phytophthora fragariae red stele root rot RPF Plant resistance to Phytophthora fragariae red stele root rot Plant The plant resistance to red stele root rot caused by Phytophthora fragariae. 1-3 ACHENEPOS scale fresh weight largest Largest fruit weight The weight of fresh fruit. Fruit LFRW DNA/std DNA_IBL Fruit ferric reducing antioxidant power FRAP Ferric reducing antioxidant power of fruit. ferric reducing antioxidant power Fruit Leaf total antioxidant assay method Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. total antioxidant content Leaf LFTOTANTIOX Leaf total antioxidant content Total antioxidant content of leaves. Flower date first relative date first relative FLDATE1REL Relative date of first open flowers for an individual, whole plant, or population. Flower 1-7 RUNNERNUM scale FLDATE2 date x 1-9 FRUITSET scale SPOAP Plant susceptibility to Podosphaera aphanis powdery mildew susceptibility to Podosphaera aphanis Plant The plant susceptibility to powdery mildew caused by Podosphaera aphanis. Meq NaOH/100g DRIP LOSS Fruit drip loss drip loss Fruit Percent weight lost when frozen berries are thawed. Fruit pH method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. 1-3 pubescence scale The plant resistance to crown rot caused by Phytophthora cactorum. Plant resistance to Phytophthora cactorum crown rot Plant RPCCR resistance to Phytophthora cactorum crown rot The percentage of achenes that are viable. ACHENEVIABLE Percentage of viable achenes Achene viable Runner total length method Harvest all runners on a single day and measure the total length. date last Flower date last FLDATE2 Flower Julian date of last flowering for an individual, whole plant, or population. date Harvest date method Record the Julian date when first fruit ripens at a specific location. Flower date first method Observe the plant and record the date the first flowers open. Resistance to Meloidogyne hapla method Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. TREXPO Truss The position of the truss relative to the foliage. Truss exposure exposure ACHENEPOS position Achene The postion of achene relative to the fruit surface. Achene position 1-9 RESIST scale Stembreak txt Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina method Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants Resistance to Neopestalotiopsis sp. scale 0-6 Pedicle breakage method Observe where the pedicle breaks during harvest. mg/L Pg-3-gluc kg−1 The soluble solids content of fruit. SSC Fruit total water soluble solid content total water soluble solid content Fruit Observe the plant and record the date when at peak flowering. Flower date 100% method RUNNERNUM Runner The number of runners. number Runner number Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Skin color 1-9 method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. Susceptibility to Phytophthora fragariae method 1-9 FRFIRM scale Observe the plant and record the reflexion of the calyx relative to the fruit shoulders Calyx reflexion method Fruit yield 1-4 method Estimate fruit yield and record it. Fruit flesh color method Observe and rate the average fruit flesh color on 3-5 fruit per genotype. DNA ration determination using DAPI with internal standard = iceberg lettuce method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. The total length of runners from a single plant harvested on a single day at a specific location. Runner total length Runner length total RTL 1-9 FLDIA UPOV scale Flower date last method Observe the plant and record the date when at the end of flowering. position CALYXPOS Calyx position Calyx The position of the calyx attachment relative to the fruit shoulder. Count the total number of daughter plants from a single plant harvested on a single day. Daughter plantlet count Plant habit scale method Observe the plant and assess the erectness or spreading of the leaves along the ground. SMYFR Plant susceptibility to Mycosphaerella fragariae leaf spot Plant The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. susceptibility to Mycosphaerella fragariae leaf spot Calyx position 1-3 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Truss exposure 1-5 method Observe the truss location relative to the foliage canopy and record it. Skin toughness The toughness of skin. toughness SKINTOUGH Skin RVD Plant resistance to Verticillium dahliae wilt resistance to Verticillium dahliae wilt Plant The plant resistance to verticillium wilt caused by Verticillium dahliae. Resistance to Phytophthora fragariae % method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. 1-10 SKINCOL UPOV scale Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Resistance to Verticillium dahliae scale 1-9 Fruit set method Observe the number of fruit that a single plant sets in a specific location. Fruit fresh weight 1 method Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Plant The plant resistance to root knot nematode Meloidogyne hapla. resistance to Meloidogyne hapla root knot nematode RMH Plant resistance to Meloidogyne hapla root knot nematode meters 1-9 SKINCOL scale Record the number of days from first flowering to ripe fruit. Fruit development method development Weekly flower evaluation FLEVAL Flower The weekly development of flowers. Skin color chromameter reflect L* brightness method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. count Skin Skin gloss gloss The glossiness of skin. SKINGLOSS Skin color 1-10 method Observe and rate the fruit skin color. Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Resistance to Mycosphaerella fragariae method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. The plant resistance to necrosis caused by Neopestalotiopsis sp. resistance to necrosis caused by Neopestalotiopsis sp. Plant RNEOP Ferric reducing antioxidant power FSH method Plant resistance to necrosis caused by Neopestalotiopsis sp. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). Fruit yield total weight method Weigh total fruit yield and record it. Plant Plant vigor vigor The vigor of plant. PLANTVIGOR Fruit shape GG method Observe and rate average fruit shape on 5 fruit per genotype. Resistance to Podosphaera aphanis % method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Meq/100g citric acid Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. Resistance to Colletotrichum acutatum anthracnose fruit rot % method Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx plug method Fruit fresh weight 5L method Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. Number of runners 1-9 method Observe and rate the number of runners per plant. 0-1 BOTRYITIS scale Plant Plant susceptibility to Phomopsis obscurans leaf blight susceptibility to Phomopsis obscurans leaf blight The plant susceptibility to leaf blight caused by Phomopsis obscurans. SPO method_of Perpetual flowering method Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. GG scale 1-5 SUSCEP scale FLDATE1 Flower date first date first Flower Julian date of first open flowers for an individual, whole plant, or population. 1-3 RESIST scale Fruit fresh weight Fruit FRFRESHWT fresh weight The weight of fresh fruit. Count the number of petals on 10 flowers and average the results Petal number average method Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides SCG The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides The plant resistance to leaf blight caused by Phomopsis obscurans. resistance to Phomopsis obscurans leaf blight RPO Plant resistance to Phomopsis obscurans leaf blight Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae % method The plant resistance to root lesion nematode Pratylenchus penetrans. Plant RPP Plant resistance to Pratylenchus penetrans root lesion nematode resistance to Pratylenchus penetrans root lesion nematode 1-9 FRSHAPUNI GRIN scale Observe fruit color uniformity from even or very slighly unevento strongly uneven. Color uniformity method 1-5 TREXPOS UPOV scale harvest date Harvest date HD Record the Julian date when first fruit ripens at a specific location. Fruit 1-3 CALYXSZ scale Measure the length of the epipodium of the primary fruit from the calyx to the truss. Epipodium measurement method Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Susceptibility to Verticillium dahliae 1-5 method Observe the truss location relative to the foliage canopy and record it. Truss exposure 0-1 method FRLEN Fruit length Fruit Length of fruit from calyx to tip. length 1-9 FRFLCOL scale 0-1 EVER scale Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina % method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Observe the number of flowers per inflorescence. Number of flowers per truss estimation Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae 0-5 method 1-3 FRCOLUNI scale 0-1 PMF scale Plant The plant resistance to root rot caused by Phytophthora cactorum. RPCRR Plant resistance to Phytophthora cactorum root rot resistance to Phytophthora cactorum root rot FLDATE100 date 100% Julian date of peak flowering for an individual, whole plant, or population. Flower date 100% Flower The total number of daughter plantlets per plant harvested on a single day at a specific location. daughters Runner DAU Runner daughters 0-1 STAMPRES scale 1-3 ACHENECOL UPOV scale The plant resistance to Mycosphaerella fragariae. Plant resistance to Mycosphaerella fragariae leaf spot Plant resistance to Mycosphaerella fragariae leaf spot RMYFR Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. Soluble solids method 1-9 STAMENHGHT scale Plant The fruit yield of one individual. Plant fruit yield fruit yield FRYIELD 1-5 RESIST scale ECOGDDREQH Plant fruit development Growing degree days to first harvest. fruit development Plant FRpH 1-9 ACHENECOL scale shape uniformity Fruit shape uniformity. Fruit shape uniformity FRSHAPEUNIF Fruit mg/L Gallic Acid EQ Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method cm Fruit pH 2 method Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Total anthocyanins Pg-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Number of runners 1-7 method Observe and rate the number of runners per plant. Fruit The effeciency of fruit set at a specific location. Fruit set FRUITSET set Weekly flowering evaluation Observe the plant weekly and record if it is flowering. 1-9 FRYIELD scale Chroma Index Skin color chromameter reflect chroma index method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Plant susceptibility to Verticillium dahliae wilt The plant susceptibility to verticillium wilt caused by Verticillium dahliae. Plant SVD susceptibility to Verticillium dahliae wilt Fruit pH Fruit pH The pH of fruit. FRpH Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method Measure the length of the fruit from the calyx to the tip and record in mm. Fruit length method mg/L Cy-3-gluc Eq Fruit fresh weight largest method Harvest and record the largest fruit over the harvest season. Reproductive factor 1-9 STAMPRES scale Measure the length of the pedicel of the primary fruit from the calyx to the truss. Pedicel measurement method Everbearing flowering method Observe the presence of flowers during each week in July, August and September. 0-1 CALPLUG scale Plant Plant resistance to Diplocarpon earlianum leaf scorch The plant resistance to leaf scorch caused by Diplocarpon earliana. resistance to Diplocarpon earlianum leaf scorch RDE 0-5 SUSCEP scale breaking habit PEDICLEBREAK Pedicle breaking habit Pedicle Pedicle breakage relative to the calyx. 1-9 FRGLOSS scale 1-7 PEDBRK scale 1-9 FLDATE1REL UPOV scale Resistance to Fusarium oxysporum f. sp. fragariae % method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. fruit yield Plant The amount of harvested fruit per plant per season. Crop estimate CE 1-3 CALYXREFLX UPOV scale Flower date first relative method Observe the plant and record the relative flowering of the first open flowers. scale_of mm Perpetual flowering RB method Record the number of weeks of flowering after week 11 from the first week of bloom. Susceptibility to Mycosphaerella fragariae method Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Observe presence of anthers and record it. Presence of stamens 0-1 method Fruit shape UPOV method Observe and rate average fruit shape on 3-5 fruit per genotype. Pedicle Stem breaking STEMBREAK Pedicle breakage relative to the calyx. breaking habit 1-9 PLHAB scale ECOGDDREQ flower development Plant Plant flower development Growing degree days to first flowering. 0-7 TRUSS scale Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape RB method Measure fruit firmness in grams using a hand-held penetrometer with a piston. Fruit firmness penetrometer method average 1-4 ACHENEPOS UPOV scale The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. Fruit resistance to Colletotrichum acutatum anthracnose fruit rot resistance to Colletotrichum acutatum anthracnose fruit rot Fruit RAFR First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Fruit yield 1-9 method Estimate fruit yield and record it. Drip loss method Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Flower diameter UPOV method Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Pedicle PEDICELLEN length Stem length of primary fruit from calyx to truss. Pedicle length Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Fruit firmness method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae 1-3 method Luminance grams 1-5 PEDUNCLEN scale Plant height Height of tallest trifoliate leaf. Plant height PLANTHT Stamen height method Observe and rate the height of the stamens relative to the receptacle at flower opening. Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis 1-9 method Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Fruit flavor method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. Titratable acidity 0.5 g citric acid method 1-9 PLANTVIGOR scale 1-4 PERPFL UPOV scale Resistance to Phytophthora cactorum CR % method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Fruit The flavor of fruit. FRFLAV Fruit flavor flavor DNA/std DNA_ILF 0-1 FLEVAL scale 1-9 FRYIELD Ordinal scale g/100g 1-5 RMH scale FLDATE1 Date Presence of stamens 1-9 method Observe presence of anthers and record it. Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. Fruit flesh firmness Fruit The firmness of fruit flesh. FRFIRM flesh firmness Titratable acidity citric acid method Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Stamen presence Stamen The presence of stamens. STAMENPRES presence variable_of Growing degree days flowering method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Folin-Ciocalteu method Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). 1-9 SKINTOUGH scale The total phenolic acids of fruit. Fruit TPHE total phenolic acids content Fruit total phenolic acids content Measure the tallest trifoliate leaf from crown to base of leaflets. Plant height method Susceptibility to Diplocarpon earlianum method Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum.