FRRAFR_FRAFR%M_% University of Florida Salinas et al. 2019 FLDIA_FLDIAM_cm UPOV GDR RosBREED FRFIRM_FRFIRMM_1-9FRFIRMscale very resistant, healthy 1.0 RFOF_RFOF%M_% USDA Zurn, et al. FLDATE100_FLDATE100M_FLDATE100date USDA FRYIELD_FRYIELD1-9M_1-9FRYIELDscale GDR RosBREED PEDICELLEN_PEDICELLENM_cm USDA GDR ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale RosBREED GDR TotalPhenolics_F-CM_mg/L_GallicAcidEQ RosBREED RPOAP_RPOAP1-9M_1-9RESISTscale USDA USDA TITA_TITA0.5M_TITAMeq/100gCA RosBREED SPOAP_SPOAPM_1-9SUSCEPscale GDR GDR RosBREED SPF_SPFM_0-5SUSCEPscale Y Stem breaks just above calyx, good for fresh market UC Davis Feldmann, et al. SVD_SVD1-5M_1-5SUSCEPscale FRUITSET_FRSETM_1-9FRUITSETscale USDA CPBR RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale UPOV Zurn, et al. RMaPh_ RMaPh%M_ % USDA oblate 7.0 USDA CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale RPCRR_RPC%M_% USDA 5FRW_FRFRESHWT5LM_g USDA GDR STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale RosBREED USDA LFRW_FRFRESHWLGM_g 3 low yield GDR RPF_RPF0-5M_0-5RESISTscale RosBREED PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale GDR RosBREED red 3 USDA FRYIELD_FRYIELD1-4M_1-4FRYIELDscale calyx attachment raised above shoulder (necked) 1 RosBREED CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale GDR USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg UPOV TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale 2 calyx size equal to fruit diameter 1 dead USDA RPCCR_RPCCR%M_% 3 protruding 3 few flowers GDR RosBREED PPMF_PPMFM_0-1PMFscale ACHENEVIABLE_ACHVIAM_% RosBREED GDR RPCCR_RPCCR1-5M_1-5RESISTscale UC Davis Jiménez, et al. FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale UPOV FRSHAPE_FRSHAPEGGM_TXT USDA FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale UPOV high 7 USDA EVERBERIN_EVER0-1M_0-1EVERscale USDA STEMBREAK_PEDBRKM_text FRpH_pH2M_pH USDA strongly below 1 FRD_FRRIPEM_days USDA GDR HARVDATE_HARVDATEM_date RosBREED DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL USDA 9 stamens taller than receptacle USDA EPILGT_EPILGTM_cm Università Politecnica delle Marche SKINCOL_REFLECTLM_L* Capocasa et al. 2008 FRpH_pHM_pH GDR RosBREED FRDEV_FRDEVM_days USDA 3 conic-wedge STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale USDA 3 calyx size larger than fruit diameter very light brown or green 9 DAU_RUNDAUM_count USDA TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA RosBREED FLEVAL_FLEVALM_0-1FLEVALscale GDR 2 divides at 25% from crown above surface 4 1 reniform GDR RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale 9 highly resistant/no symptoms FRLEN_FRLENM_mm USDA resistant R 7 pedicle breaks well above calyx USDA CE_CROPEST_g PLANTHT_PLANTHTM_cm USDA USDA RPF_RPF%M_% GDR RosBREED ECOGDDREQ_GDDFM_days RosBREED FRFLCOL_FRFLCOLM_1-9FRFLCOLscale GDR RVD_RVD%_% USDA 2 medium yield HD_FIRSTHARVM_date USDA divides right before fruit 5.0 ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA calyx size smaller than fruit diameter 1 partially remontant 2 GDR SKINGLOSS_SKINGLOSSM_1-9GLOSSscale RosBREED RosBREED TRUSSSIZ_TRUSSCOUNTM_ave GDR 6.0 medium to large USDA RUNNERDATE_FRUNNERM_date Pinkerton & Finn RPP_RPPM_Rf NEM-RF_RPPM_Rf USDA Capocasa et al. 2008 FRAP_FRAPFSHM_FSHEQumoles/g fruit Università Politecnica delle Marche USDA RDE_REDM_1-9RESISTscale RFOF_RFOF1-5M_1-5RESISTscale Pincot, et al. UC Davis FLDIA_FLDIAM_1-9FLDIAUPOVscale UPOV calyx attachment is sunken below shoulder 5 highly susceptible/severe symptoms 1 USDA FLDATE1_FLDATE1M_FLDATE1date 1 highly resistant/no symptoms 6.0 pink NCRI RPCCR_RPCCR1-8M_1-8RESISTscale Eikemo, et al. CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale GDR RosBREED USDA FRRP_FRRPM_Ratio GDR SMYFR_SMYFRM_1-9SUSCEPscale RosBREED highly resistant/no symptoms 9 slightly symptomatic 1.5 TITA_TITAM_TITAMeq/100gCA GDR RosBREED 1 no flowers STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale USDA RosBREED CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale GDR BEARINGTYPE_BEARINGRBM_count GDR RosBREED GDR RosBREED TREXPOS_TREXPOS0-1M_0-1TREXPOSscale no fruit set 1 UPOV CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale 1 highly susceptible/severe symptoms USDA RTL_RUNTOTLENM_m fully remontant 3 1 pedicle does not break 1 high density 9.0 wedged 1 white circular 8.0 7.0 light red 7.0 large FLDATE2_FLDATE2M_date USDA long conic 1 3 small 10.0 blackish red RosBREED GDR RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale 6.0 rhombic RVD_RVD1-3M_1-3RESISTscale Oregon State Vining, et al. dark 1 RosBREED PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale GDR 8 highly susceptible/severe symptoms many 7 PLANTHABIT_PLHABM_1-9PLHABscale USDA 5.0 oblong very sweet, no off-flavors 9 GDR SPO_SPOM_1-9SUSCEPscale RosBREED RosBREED FRFRESHWT_FRFRESHWT5M_g GDR BLDATE1_FLDATE1M_FLDATE1date USDA SCG_SCGWKM_R-Scategory University of Florida Chandra et al. 2021 PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale USDA 4 small to medium Capocasa et al. 2008 SKINCOL_REFLECTCIXM_ChromaIndex Università Politecnica delle Marche 5 intermediate low density 3 9 blackish red Knapp, et al. RMaPh_ RMaPhM_1-5RESISTscale UC Davis cordiform 7 Strawberry ontology 9.0 very late plant has stamens 9 entity even 2 7 late FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale CPBR USDA RPOAP_RPOAP%M_% 4 medium orange 1 very early slightly uneven 2 yellowish white 2 3 light orange 5.0 medium ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq GDR RosBREED ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV 5 pedicle breaks just above calyx Università Politecnica delle Marche FRFIRM_PENEM_g Capocasa et al. 2008 erect 9 same level 3 2 very small to small RosBREED GDR FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale GDR RosBREED FRFLAV_FRFLAVM_1-9FRFLAVscale USDA PLOIDY_FCDAPIICFM_x GDR SDE_SDEM_1-9SUSCEPscale RosBREED ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale UPOV DRIPLOSS_DRIPLOSSM_g GDR RosBREED Fernandez et al. RNEOP_RNEOP0-6M_0-6RESISTscale University of North Carolina 2 slightly below surface GDR SKINCOL_SKINCOL1-9M_1-9SKINCOLscale RosBREED 1 no fruit 9 very firm Capocasa et al. 2008 TITA_TITAM_TITAMeqNAOH/100g Università Politecnica delle Marche 2 semi-upright UPOV BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale USDA FRCOL1_FRCOL1M_date 5 globose-conic plant has no stamens 1 GDR SSC_SSCM_SSCg/100g RosBREED Università Politecnica delle Marche FRAP_FRAPTROM_TroloxEQumoles/g FW Capocasa et al. 2008 University of Delaware LFPUBESC_LFPUBESCM_1-3LFPUBESCscale Biswas et al. 2019 divides 75% from crown 4 DNARATIO_DNADAPIICFM_DNA/stdDNAILF USDA dull 1 5 strongly uneven very soft 1 USDA RPO_RPOM_% 5.0 globose-conic GDR RosBREED FRFLCOLDEPTH_FRCOLDEPTHM_% Biswas et al. 2019 LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW University of Delaware PLOIDY_FCDAPIIBLM_x USDA completely uniform/symmetrical and attractive 9 strongly below surface 1 FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale USDA very glossy 9 orange red 5.0 slightly uneven 3 USDA FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale globose 3 1 not uniform/malformed 1 highly resistant/no symptoms 2 level with fruit no fruit 1 3 raised 3 downwards no runners 1 susceptible S University of California, Davis Pincot et al. 2020 RVD_RVD1-9M_1-9RESISTScale CPBR PLANTHABIT_PLHABM_1-3PLHABscale 9 hundreds 1 high yield 3 level with surface Y** stem breaks well above calyx USDA FRYIELD_FRYIELDTWM_g pinkish white 1 USDA RMH_RMHM_1-5RMHscale Pinkerton & Finn 1 not remontant no fruit set 4 highly susceptible/severe symptoms 9 USDA RMYFR_RMYFRM_% few 3 9 complete fruit set 9 fully reflexed 1 upwards GDR ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale RosBREED white 1 3 calyx attachment is even with fruit 9.0 dark red USDA BOTRYTIS_RBCM_0-1BOTRYTISscale 2 outwards upright 1 1 greenish mildly symptomatic 2.0 V variable, no consistent breaking habit medium density 2 dark red 9 tough 9 stem does not break, berry separates from calyx easily N overcropped 9 1 even CALYXSZ_CALYXSZM_1-3CALYXSZscale GDR RosBREED 1 highly resistant/no symptoms strongly above 5.0 long conic 2 slightly above 4 slightly below 2 4 ovate very symptomatic 2.5 RosBREED ECOGDDREQH_GDDHM_days GDR very large 9.0 medium 5 UPOV TRUSSSIZ_TRUSSM_1-9scale RUNNERNUM_RUNNERNUMCT_count USDA not reflexed 1 1 conic 1 stamens shorter than receptacle 3 strongly uneven absent or very weak 1 3 low early 3 SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale UPOV acronym many flowers 7 3 divides 50% from crown 6.0 fingerling UPOV FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale 3 spreading 1 sunken very easily removed 9 2 conic 9.0 very high globose 4 even or very slightly uneven 1 extremely vigorous 9 medium 5 8.0 large to very large not sweet, bad off-flavors 1 8.0 medium red does not remove 1 breakage is inconsistent 3 1 inserted attribute 1 very small cordate 3 2 yellow FLPETALNUM_FLPETALNUMM_average USDA 4 day neutral 1 spreading soft 1 highly susceptible/severe symptoms 5 divides close to crown 1 3.0 dead medium number of flowers 5 9.0 oblate date Observe presence of anthers and record it. Presence of stamens 1-9 method titratable acidity TITA Fruit titratable acidity The titratable acidity content of fruit. Fruit Count the total number of daughter plants from a single plant harvested on a single day. Daughter plantlet count Growing degree days to first flowering. Plant ECOGDDREQ Plant flower development flower development STAMENPRES Stamen Stamen presence The presence of stamens. presence Plant habit habit Plant PLANTHABIT The degree of erectness of the leaves. Runner total length method Harvest all runners on a single day and measure the total length. The position of the truss relative to the foliage. Truss exposure TREXPO exposure Truss % FLDATE1 Date Skin toughness SKINTOUGH The toughness of skin. Skin toughness Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-3 method Titratable acidity citric acid method Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Skin color on the sun side of a fully ripe fruit. color SKINCOL Skin Skin color Resistance to Macrophomina phaseolina method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. 1-9 ACHENECOL scale 1-9 CALYXREM scale Number of runners 1-7 method Observe and rate the number of runners per plant. Record the Julian date when first fruit ripens at a specific location. Fruit Harvest date harvest date HD FLEVAL The weekly development of flowers. Weekly flower evaluation development Flower FRLEN length Fruit length Length of fruit from calyx to tip. Fruit The plant resistance to powdery mildew caused by Podosphaera aphanis. Plant resistance to Podosphaera aphanis powdery mildew RPOAP Plant resistance to Podosphaera aphanis powdery mildew Folin-Ciocalteu method Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). mm PLANTVIGOR vigor Plant The vigor of plant. Plant vigor Weekly flowering evaluation Observe the plant weekly and record if it is flowering. Record the number of days from first flowering to ripe fruit. Fruit development method average Pedicle breaking habit Pedicle breaking habit PEDICLEBREAK Pedicle breakage relative to the calyx. Susceptibility to Phytophthora fragariae method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. 5FRW Fruit fresh weight The weight of fresh fruit. Fruit weight grams Resistance to Diplocarpon earlianum method Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. 1-9 CALYXREFLX scale Fruit The firmness of fruit flesh. FRFIRM flesh firmness Fruit flesh firmness resistance to necrosis caused by Neopestalotiopsis sp. Ferric reducing antioxidant power FSH method The plant resistance to necrosis caused by Neopestalotiopsis sp. Plant Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). RNEOP Plant resistance to necrosis caused by Neopestalotiopsis sp. resistance to Phomopsis obscurans leaf blight Plant resistance to Phomopsis obscurans leaf blight RPO The plant resistance to leaf blight caused by Phomopsis obscurans. DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method 1-8 RESIST scale g/100g resistance to Diplocarpon earlianum leaf scorch Plant resistance to Diplocarpon earlianum leaf scorch The plant resistance to leaf scorch caused by Diplocarpon earliana. RDE Plant 1-3 CALYXREFLX UPOV scale Percent weight lost when frozen berries are thawed. Fruit DRIP LOSS drip loss Fruit drip loss 1-9 STAMENHGHT scale Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method Fruit pH method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. Number of flowers per truss estimation Observe the number of flowers per inflorescence. Observe and rate average fruit shape on 5 fruit per genotype. Fruit shape GG method Plant SVD The plant susceptibility to verticillium wilt caused by Verticillium dahliae. Plant susceptibility to Verticillium dahliae wilt susceptibility to Verticillium dahliae wilt Peduncle length method Observe average peduncle length and record it. Growing degree days harvest method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Susceptibility to Podosphaera aphanis method Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. The percentage of achenes that are viable. viable Achene Percentage of viable achenes ACHENEVIABLE Observe the plant and record the date when at peak flowering. Flower date 100% method RFOF The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Plant resistance to Fusarium oxysporum f. sp. fragariae wilt Plant resistance to Fusarium oxysporum f. sp. fragariae wilt flesh depth of color FRFLCOLDEPTH Depth of fruit flesh color. Fruit Fruit flesh depth of color resistance to Macrophomina phaseolina charcoal rot Plant The plant resistance to charcoal rot caused by Macrophomina phaseolina. RMaPh Plant resistance to Macrophomina phaseolina charcoal rot Fruit shape shape FRSHAPE Fruit The shape of fruit. 1-9 SKINTOUGH scale Resistance to Phytophthora cactorum CR 1-5 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. 1-9 PLANTVIGOR scale 1-5 RESIST scale Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. SSC:TA method Fruit appearance method Observe fruit appearance from not uniform to completely uniform. Estimate fruit yield and record it. Fruit yield 1-9 O method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-3 method Crop estimate method Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Observe the truss location relative to the foliage canopy and record it. Truss exposure 0-1 method Chroma Index total anthocyanin content Fruit total anthocyanin content The total anthocyanins of fruit. Fruit ATCYN Reproductive factor Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Fruit flavor method 1-9 SUSCEP scale Flower date first relative method Observe the plant and record the relative flowering of the first open flowers. 1-3 CALYXPOS UPOV scale Flower date first method Observe the plant and record the date the first flowers open. resistance to Phytophthora fragariae red stele root rot RPF Plant Plant resistance to Phytophthora fragariae red stele root rot The plant resistance to red stele root rot caused by Phytophthora fragariae. Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Resistance to Meloidogyne hapla method Fruit The weight of fresh fruit. Largest fruit weight fresh weight largest LFRW Observe presence of anthers and record it. Presence of stamens 0-1 method R-S category length Pedicle length Stem length of primary fruit from calyx to truss. Pedicle PEDICELLEN total antioxidant content Leaf Leaf total antioxidant content LFTOTANTIOX Total antioxidant content of leaves. 1-4 ACHENEPOS UPOV scale cm 0-6 RESIST scale RMH resistance to Meloidogyne hapla root knot nematode The plant resistance to root knot nematode Meloidogyne hapla. Plant resistance to Meloidogyne hapla root knot nematode Plant size Calyx CALYXSZ Calyx size The size of the calyx. Fruit flavor The flavor of fruit. flavor FRFLAV Fruit 1-9 FRYIELD Ordinal scale 1-10 SKINCOL UPOV scale GG scale resistance to Phytophthora cactorum root rot The plant resistance to root rot caused by Phytophthora cactorum. Plant resistance to Phytophthora cactorum root rot RPCRR Plant Fruit yield 1-9 method Estimate fruit yield and record it. Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. Resistance to Colletotrichum acutatum anthracnose fruit rot % method Estimate fruit yield and record it. Fruit yield 1-4 method Calyx reflexion method Observe the plant and record the reflexion of the calyx relative to the fruit shoulders Resistance to Macrophomina phaseolina % method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Calyx Calyx reflexion CALYXREFLX reflexion Reflexion of the calyx relative to the fruit shoulders. Titratable acidity 0.5 g citric acid method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. Plant Plant ploidy ploidy The number of sets of chromosomes in the nucleus. PLOIDY color Achene The color of achene on the sun side of a fully ripe fruit. ACHENECOL Achene color Fruit yield total weight method Weigh total fruit yield and record it. 1-5 RMH scale 1-9 FRFLCOL scale Plant resistance to Pratylenchus penetrans root lesion nematode RPP Plant The plant resistance to root lesion nematode Pratylenchus penetrans. resistance to Pratylenchus penetrans root lesion nematode Leaf LFPUBESC pubescence Leaf pubescence The density of trichomes on the leaf surface. Number of runners 1-9 method Observe and rate the number of runners per plant. Plant RVD resistance to Verticillium dahliae The plant resistance to verticillium wilt caused by Verticillium dahliae. Plant resistance to Verticillium dahliae wilt Meq NaOH/100g Achene position The postion of achene relative to the fruit surface. Achene position ACHENEPOS Stamen height method Observe and rate the height of the stamens relative to the receptacle at flower opening. Observe the plant and record the Julian date the first runners emerge at a specific location. First runner date method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR % method 1-9 SKINCOL scale Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. Average flower petal number. Flower petal number Flower petal number FLPETALNUM mg/L Cy-3-gluc Eq The amount of harvested fruit per plant per season. fruit yield Crop estimate CE Plant Percentage of viable achenes method Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. DNA ration determination using DAPI with internal standard = iceberg lettuce method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Runner daughters DAU Runner The total number of daughter plantlets per plant harvested on a single day at a specific location. daughters Fruit Appearance of first color on fruit Appearance of first color on fruit. first color appearance FRCOL1 Calyx position 1-3 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. 1-9 RESIST scale 1-3 CALYXSZ scale Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. 1-7 PEDBRK scale days 1-5 FRCOLUNI scale Leaf total antioxidant assay method Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Titratable acidity NaOH method Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Pedicel measurement method Measure the length of the pedicel of the primary fruit from the calyx to the truss. Flower diameter UPOV method Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Harvest date method Record the Julian date when first fruit ripens at a specific location. STAMENHGHT Stamen height Stamen height The height of the stamens relative to the receptacles. Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Susceptibility to Diplocarpon earlianum method 1-9 FRGLOSS scale 1-9 FRFIRM scale Fruit harvest date Record the Julian date when first fruit ripens at a specific location. harvest date HARVDATE Fruit Plant Everbearing EVERBERIN bearing type Whether or not the plant has an everbearing habit Susceptibility to Phomopsis obscurans method Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Fruit ripening trait FRRP Fruit ripening trait The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA Skin gloss gloss Skin SKINGLOSS The glossiness of skin. Fruit ripen days Number of days from flowering to ripe fruit. development FRD Fruit Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface 1-9 FRFLAV scale Petal number average method Count the number of petals on 10 flowers and average the results Runner number The number of runners. RUNNERNUM Runner number Fruit uniformity of color uniformity of color Fruit uniformity of color. FRCOLORUNIF Fruit FLDATE100 Flower date 100% Flower date 100% Julian date of peak flowering for an individual, whole plant, or population. Trolox EQ μmoles/g FW g Peduncle length length Peduncle PEDUNCLEN The length of peduncle. FLDATE2 date Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae 1-3 method FRDEV Fruit development development Fruit Number of days from flowering to ripe fruit. Fruit set set Fruit FRUITSET The effeciency of fruit set at a specific location. The soluble solids content of fruit. Fruit total water soluble solid content total water soluble solid content SSC Fruit Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method Record the number of weeks of flowering after week 11 from the first week of bloom. Perpetual flowering RB method Observe the presence of flowers during each week in July, August and September. Everbearing flowering method presence of grey mold The presence of grey mold caused by Botrytis cinerea on the plant or fruit. BOTRYTIS Plant Indication of resistance to Botrytis Soluble solids method Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. Stembreak txt Achene color 1-9 method Observe the average achene color on 3-5 fruit per genotype and record it. Skin color color SKINCOL Skin color on the sun side of a fully ripe fruit. Skin Observe where the pedicle breaks during harvest. Pedicle breakage method Relative date of first open flowers for an individual, whole plant, or population. Flower date first relative FLDATE1REL Flower date first relative FLDATE100 date Resistance to Fusarium oxysporum f. sp. fragariae % method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Achene position 1-4 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. mg/L Gallic Acid EQ ECOGDDREQH fruit development Plant fruit development Growing degree days to first harvest. Plant Fruit Total anthocyanins TACY The total anthocyanins of fruit. total anthocyanin content 0-1 FLEVAL scale Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Skin color chromameter reflect chroma index method The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. resistance to Colletotrichum acutatum anthracnose fruit rot RAFR Fruit resistance to Colletotrichum acutatum anthracnose fruit rot Fruit The pH of fruit. pH Fruit pH Fruit FRpH The diameter of flower at fully open. Flower diameter diameter Flower FLDIA Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Skin color chromameter reflect L* brightness method Resistance to Verticillium dahliae % method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. RMYFR Plant resistance to Mycosphaerella fragariae leaf spot Plant The plant resistance to Mycosphaerella fragariae. resistance to Mycosphaerella fragariae leaf spot Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. presence of powdery mildew PPMF Fruit presence of powdery mildew The presence of powdery mildew on fruit. Fruit Runner RTL length total The total length of runners from a single plant harvested on a single day at a specific location. Runner total length Fruit fresh weight largest method Harvest and record the largest fruit over the harvest season. FRpH 0-1 PMF scale Ratio Depth of fruit flesh color Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. Plant height method Measure the tallest trifoliate leaf from crown to base of leaflets. Luminance Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Ease of calyx removal method susceptibility to Podosphaera aphanis The plant susceptibility to powdery mildew caused by Podosphaera aphanis. SPOAP Plant Plant susceptibility to Podosphaera aphanis powdery mildew Pedicle breakage relative to the calyx. STEMBREAK breaking habit Pedicle Stem breaking Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants Resistance to Neopestalotiopsis sp. scale 0-6 FRAP Fruit ferric reducing antioxidant power Ferric reducing antioxidant power of fruit. Fruit ferric reducing antioxidant power Flower date last method Observe the plant and record the date when at the end of flowering. Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis % method mg/L Pg-3-gluc kg−1 Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Ferric reducing antioxidant power trolox method Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). size Truss Truss size TRUSSSIZ Number of flowers per truss Fruit total phenolic acids content The total phenolic acids of fruit. TPHE total phenolic acids content Fruit Julian date of first open flowers for an individual, whole plant, or population. date first Flower BDF Bloom date first Plant height PLANTHT Height of tallest trifoliate leaf. Plant height EPILGT Stem length of primary fruit from calyx to truss. Epipodium length length Epipodium 1-4 PERPFL UPOV scale Fruit shape RB method Observe and rate average fruit shape on 3-5 fruit per genotype. Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Skin color 1-9 method Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant SCG Plant fruit yield FRYIELD fruit yield The fruit yield of one individual. Plant Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Pg-3-gluc method Plant The plant susceptibility to red stele root rot caused by Phytophthora fragariae. susceptibility to Phytophthora fragariae red stele root rot Plant susceptibility to Phytophthora fragariae red stele root rot SPF date last Flower date last FLDATE2 Julian date of last flowering for an individual, whole plant, or population. Flower Observe the number of fruit that a single plant sets in a specific location. Fruit set method Fruit length method Measure the length of the fruit from the calyx to the tip and record in mm. 1-3 ACHENEPOS scale Plant susceptibility to Mycosphaerella fragariae leaf spot SMYFR The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. Plant susceptibility to Mycosphaerella fragariae leaf spot Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. Plant BEARINGTYPE bearing type Plant bearing type The plant resistance to crown rot caused by Phytophthora cactorum. RPCCR Plant resistance to Phytophthora cactorum crown rot Plant resistance to Phytophthora cactorum crown rot Observe fruit color uniformity from even or very slighly unevento strongly uneven. Color uniformity method The plant resistance to verticillium wilt caused by Verticillium dahliae. Plant resistance to Verticillium dahliae wilt resistance to Verticillium dahliae wilt Plant RVD Observe the plant and assess the erectness or spreading of the leaves along the ground. Plant habit scale method Julian date of first open flowers for an individual, whole plant, or population. date first Flower FLDATE1 Flower date first 1-9 RUNNERNUM scale Plant susceptibility to leaf scorch caused by Diplocarpon earliana The plant susceptibility to leaf scorch caused by Diplocarpon earliana SDE Plant susceptibility to Diplocarpon earliana Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. Calyx size method Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Drip loss method Observe the presence of powdery mildew on ripe fruit. Presence of powdery mildew on fruit method 0-1 TREXPOS scale 1-9 STAMPRES scale 0-1 EVER scale 1-3 ACHENECOL UPOV scale Fruit The firmness of fruit flesh. flesh firmness FRFIRM Fruit flesh firmness Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Resistance to Mycosphaerella fragariae method 1-9 FLDIA UPOV scale 1-5 CALYXPOS scale Observe plant vigor of the two clones and record the average. Plant vigor method Susceptibility to Mycosphaerella fragariae method Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. titratable acidity Fruit TITA Fruit titratable acidity The titratable acidity content of fruit. Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. Measure the diameter of the flowers at fully open Flower diameter method CALYXPLUG Calyx plug plug Presence or absence of central plug after calyx removal. Calyx fresh weight The weight of fresh fruit. FRFRESHWT Fruit Fruit fresh weight Fruit flesh color method Observe and rate the average fruit flesh color on 3-5 fruit per genotype. 1-3 RESIST scale first emergence Runner The Julian date of appearance of the first runners. First runner date RUNNERDATE Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Fruit firmness method Calyx CALYXREMOVE Calyx ease of removal Ease of calyx removal. ease of removal 1-3 pubescence scale Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days flowering method Fruit fresh weight method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. 1-5 TREXPOS UPOV scale 1-9 PLHAB scale Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis 1-9 method Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight 1 method Measure the length of the epipodium of the primary fruit from the calyx to the truss. Epipodium measurement method 1-9 FRSHAPUNI GRIN scale 1-9 FRSHAPE UPOV scale Resistance to Phytophthora fragariae 0-5 method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Perpetual flowering method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method 1-5 PEDUNCLEN scale meters Observe and rate the fruit skin color. Skin color 1-10 method DNA/std DNA_IBL Total anthocyanins Cy-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Susceptibility to Verticillium dahliae 1-5 method Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. 0-5 SUSCEP scale Resistance to Phytophthora fragariae % method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. DNA/std DNA_ILF Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape UPOV method 1-9 FRSHAPE RB scale 1-9 FRYIELD scale Plant DNA ratio Ratio of sample DNA to standard DNA. DNA ratio DNARATIO Plant FRSHAPEUNIF Fruit shape uniformity. Fruit shape uniformity Fruit shape uniformity scale_of 0-1 STAMPRES scale CALYXPOS The position of the calyx attachment relative to the fruit shoulder. Calyx position Calyx position Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. 1-9 FLDATE1REL UPOV scale 0-1 BOTRYITIS scale Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-5 method Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. Fruit fresh weight 5L method FSH EQ μmoles/g fruit Resistance to Verticillium dahliae scale 1-9 Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms The plant susceptibility to leaf blight caused by Phomopsis obscurans. SPO susceptibility to Phomopsis obscurans leaf blight Plant susceptibility to Phomopsis obscurans leaf blight Plant Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Fruit pH 2 method Observe the truss location relative to the foliage canopy and record it. Truss exposure 1-5 method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Susceptibility to Colletotrichum gloeosporioides weekly method Measure fruit firmness in grams using a hand-held penetrometer with a piston. Fruit firmness penetrometer method count 1-9 FRUITSET scale Meq/100g citric acid 0-1 CALPLUG scale method_of variable_of FRFLCOL The color of fruit flesh. Fruit flesh color Fruit flesh color 1-4 FRYIELD scale Number of runners count method Harvest and count the number of runners collected from a single crown on a single day in a specific location. 1-5 SUSCEP scale x Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx plug method 1-7 RUNNERNUM scale 1-3 FRCOLUNI scale 1-3 PLHAB scale 0-7 TRUSS scale