FLDIA_FLDIAM_1-9FLDIAUPOVscale UPOV GDR CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale RosBREED FRAP_FRAPTROM_TroloxEQumoles/g FW Capocasa et al. 2008 Università Politecnica delle Marche FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale USDA 2 yellowish white large to very large 8.0 1 sunken Capocasa et al. 2008 Università Politecnica delle Marche SKINCOL_REFLECTCIXM_ChromaIndex USDA RPCCR_RPCCR%M_% GDR RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale RosBREED outwards 2 UC Davis RPCCR_RPCCR1-5M_1-5RESISTscale Jiménez, et al. RPCCR_RPCCR1-8M_1-8RESISTscale Eikemo, et al. NCRI FLDATE1_FLDATE1M_FLDATE1date USDA BLDATE1_FLDATE1M_FLDATE1date USDA overcropped 9 divides 50% from crown 3 USDA FRYIELD_FRYIELDTWM_g BOTRYTIS_RBCM_0-1BOTRYTISscale USDA FLDIA_FLDIAM_cm UPOV USDA FRCOL1_FRCOL1M_date USDA LFRW_FRFRESHWLGM_g 9.0 dark red USDA RPF_RPF%M_% SCG_SCGWKM_R-Scategory University of Florida Chandra et al. 2021 conic-wedge 3 TotalPhenolics_F-CM_mg/L_GallicAcidEQ RosBREED GDR DAU_RUNDAUM_count USDA 9 completely uniform/symmetrical and attractive USDA 5FRW_FRFRESHWT5LM_g SKINCOL_SKINCOL1-9M_1-9SKINCOLscale RosBREED GDR 7 many RosBREED GDR HARVDATE_HARVDATEM_date calyx attachment is even with fruit 3 UPOV FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale FRFIRM_FRFIRMM_1-9FRFIRMscale RosBREED GDR 1 even or very slightly uneven UPOV TRUSSSIZ_TRUSSM_1-9scale FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale UPOV UPOV TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale 9.0 very high no fruit set 4 high density 1 ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV FRFLAV_FRFLAVM_1-9FRFLAVscale RosBREED GDR ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale RosBREED GDR GDR SMYFR_SMYFRM_1-9SUSCEPscale RosBREED RosBREED ECOGDDREQH_GDDHM_days GDR ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale RosBREED GDR SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale RosBREED GDR RosBREED GDR FRYIELD_FRYIELD1-9M_1-9FRYIELDscale oblate 7.0 STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale USDA 9.0 very late blackish red 10.0 highly susceptible/severe symptoms 5 GDR SSC_SSCM_SSCg/100g RosBREED RosBREED GDR RPF_RPF0-5M_0-5RESISTscale RosBREED TRUSSSIZ_TRUSSCOUNTM_ave GDR erect 9 1 highly resistant/no symptoms 1 highly susceptible/severe symptoms USDA FRYIELD_FRYIELD1-4M_1-4FRYIELDscale RosBREED SPO_SPOM_1-9SUSCEPscale GDR FRDEV_FRDEVM_days USDA 1 absent or very weak ECOGDDREQ_GDDFM_days RosBREED GDR USDA RDE_REDM_1-9RESISTscale ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA semi-upright 2 high yield 1 5 pedicle breaks just above calyx FRD_FRRIPEM_days USDA CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale UPOV CALYXSZ_CALYXSZM_1-3CALYXSZscale RosBREED GDR 3 slightly uneven no flowers 1 not reflexed 1 USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg 5.0 oblong even 2 1 pinkish white RosBREED GDR SDE_SDEM_1-9SUSCEPscale 9.0 oblate GDR ACHENEVIABLE_ACHVIAM_% RosBREED GDR TREXPOS_TREXPOS0-1M_0-1TREXPOSscale RosBREED USDA PLOIDY_FCDAPIIBLM_x stem breaks well above calyx Y** USDA FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale Università Politecnica delle Marche TITA_TITAM_TITAMeqNAOH/100g Capocasa et al. 2008 USDA EVERBERIN_EVER0-1M_0-1EVERscale USDA TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq hundreds 9 R resistant USDA RMH_RMHM_1-5RMHscale Pinkerton & Finn 1 plant has no stamens spreading 3 RFOF_RFOF%M_% USDA Zurn, et al. 1 stamens shorter than receptacle FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale RosBREED GDR 5 intermediate SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale UPOV USDA FRRP_FRRPM_Ratio USDA FLPETALNUM_FLPETALNUMM_average RUNNERDATE_FRUNNERM_date USDA GDR STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale RosBREED V variable, no consistent breaking habit USDA RPOAP_RPOAP1-9M_1-9RESISTscale 6.0 fingerling TITA_TITA0.5M_TITAMeq/100gCA USDA RosBREED FLEVAL_FLEVALM_0-1FLEVALscale GDR 3 few Pincot et al. 2020 RVD_RVD1-9M_1-9RESISTScale University of California, Davis 7 pedicle breaks well above calyx low 3 6.0 pink RVD_RVD%_% USDA Feldmann, et al. SVD_SVD1-5M_1-5SUSCEPscale UC Davis plant has stamens 9 medium 5 USDA RUNNERNUM_RUNNERNUMCT_count medium 5 SPF_SPFM_0-5SUSCEPscale RosBREED GDR Strawberry ontology University of Delaware Biswas et al. 2019 LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW RosBREED SPOAP_SPOAPM_1-9SUSCEPscale GDR USDA PEDICELLEN_PEDICELLENM_cm USDA FLDATE2_FLDATE2M_date stem does not break, berry separates from calyx easily N 3 small very small 1 HD_FIRSTHARVM_date USDA even 1 1 dull 2 divides at 25% from crown USDA STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale CPBR FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale cordiform 7 DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL USDA 5.0 orange red 1 not remontant PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale USDA Pincot, et al. UC Davis RFOF_RFOF1-5M_1-5RESISTscale yellow 2 CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale RosBREED GDR slightly symptomatic 1.5 Università Politecnica delle Marche Capocasa et al. 2008 FRFIRM_PENEM_g 1 highly resistant/no symptoms UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale attribute 1 upwards medium to large 6.0 RosBREED GDR ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq very sweet, no off-flavors 9 RMYFR_RMYFRM_% USDA small to medium 4 UPOV FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale 5.0 strongly above USDA RMaPh_ RMaPh%M_ % Zurn, et al. 1 does not remove USDA PLOIDY_FCDAPIICFM_x 3.0 dead long conic 2 highly susceptible/severe symptoms 1 Salinas et al. 2019 FRRAFR_FRAFR%M_% University of Florida UPOV BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale 3 downwards FRSHAPE_FRSHAPEGGM_TXT USDA 5 strongly uneven soft 1 medium red 8.0 RosBREED GDR DRIPLOSS_DRIPLOSSM_g FRpH_pH2M_pH USDA Y Stem breaks just above calyx, good for fresh market University of North Carolina Fernandez et al. RNEOP_RNEOP0-6M_0-6RESISTscale raised 3 cordate 3 entity GDR SKINGLOSS_SKINGLOSSM_1-9GLOSSscale RosBREED level with surface 3 very firm 9 USDA FRUITSET_FRSETM_1-9FRUITSETscale globose 4 USDA EPILGT_EPILGTM_cm USDA RPO_RPOM_% long conic 1 Biswas et al. 2019 University of Delaware LFPUBESC_LFPUBESCM_1-3LFPUBESCscale divides right before fruit 5.0 9 blackish red RosBREED GDR FRFLCOLDEPTH_FRCOLDEPTHM_% CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale RosBREED GDR 1 white spreading 1 9 highly resistant/no symptoms very small to small 2 USDA DNARATIO_DNADAPIICFM_DNA/stdDNAILF 2 medium yield RPOAP_RPOAP%M_% USDA CPBR RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale Capocasa et al. 2008 FRAP_FRAPFSHM_FSHEQumoles/g fruit Università Politecnica delle Marche slightly uneven 2 USDA PLANTHABIT_PLHABM_1-9PLHABscale 5.0 globose-conic 1 no runners calyx attachment is sunken below shoulder 5 highly susceptible/severe symptoms 8 RPCRR_RPC%M_% USDA red 3 no fruit set 1 FRLEN_FRLENM_mm USDA 1 no fruit CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale USDA high 7 USDA CE_CROPEST_g 7 many flowers RMaPh_ RMaPhM_1-5RESISTscale Knapp, et al. UC Davis dark 1 Pinkerton & Finn USDA RPP_RPPM_Rf NEM-RF_RPPM_Rf 1 pedicle does not break 7 late 3 low density 5.0 medium 1 inserted RosBREED PPMF_PPMFM_0-1PMFscale GDR 1 white slightly below 2 1 highly resistant/no symptoms 9 very easily removed USDA FLDATE100_FLDATE100M_FLDATE100date 9 tough RosBREED BEARINGTYPE_BEARINGRBM_count GDR rhombic 6.0 RTL_RUNTOTLENM_m USDA light orange 3 FRpH_pHM_pH RosBREED GDR conic 1 PLANTHABIT_PLHABM_1-3PLHABscale CPBR 1 reniform very resistant, healthy 1.0 USDA STEMBREAK_PEDBRKM_text RVD_RVD1-3M_1-3RESISTscale Vining, et al. Oregon State 8.0 circular RosBREED GDR FRFLCOL_FRFLCOLM_1-9FRFLCOLscale not sweet, bad off-flavors 1 light red 7.0 3 few flowers 7.0 large globose 3 strongly uneven 3 calyx size larger than fruit diameter 3 PLANTHT_PLANTHTM_cm USDA 3 same level Capocasa et al. 2008 SKINCOL_REFLECTLM_L* Università Politecnica delle Marche fully reflexed 9 GDR PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale RosBREED RosBREED GDR PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale UPOV CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale 1 very early 9 very light brown or green slightly below surface 2 calyx size equal to fruit diameter 2 conic 2 3 fully remontant dead 1 upright 1 9.0 wedged 9 highly resistant/no symptoms calyx size smaller than fruit diameter 1 low yield 3 very glossy 9 2 level with fruit 3 protruding susceptible S 1 greenish RosBREED GDR FRFRESHWT_FRFRESHWT5M_g 4 ovate 9.0 very large medium number of flowers 5 medium orange 4 slightly above 4 1 strongly below 1 calyx attachment raised above shoulder (necked) partially remontant 2 extremely vigorous 9 early 3 divides 75% from crown 4 1 divides close to crown TITA_TITAM_TITAMeq/100gCA RosBREED GDR globose-conic 5 2 medium density dark red 9 day neutral 4 2.0 mildly symptomatic highly susceptible/severe symptoms 9 breakage is inconsistent 3 9 stamens taller than receptacle complete fruit set 9 no fruit 1 above surface 4 1 not uniform/malformed very symptomatic 2.5 very soft 1 acronym strongly below surface 1 Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days flowering method Plant resistance to Fusarium oxysporum f. sp. fragariae wilt RFOF The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Plant resistance to Fusarium oxysporum f. sp. fragariae wilt flesh firmness Fruit flesh firmness The firmness of fruit flesh. Fruit FRFIRM Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. fresh weight largest The weight of fresh fruit. Largest fruit weight LFRW Fruit 0-1 PMF scale Plant The fruit yield of one individual. Plant fruit yield fruit yield FRYIELD Fruit Fruit weight 5FRW fresh weight The weight of fresh fruit. 0-1 TREXPOS scale 1-4 PERPFL UPOV scale Plant Crop estimate The amount of harvested fruit per plant per season. fruit yield CE g Harvest all runners on a single day and measure the total length. Runner total length method SVD Plant susceptibility to Verticillium dahliae wilt Plant susceptibility to Verticillium dahliae wilt The plant susceptibility to verticillium wilt caused by Verticillium dahliae. Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Susceptibility to Mycosphaerella fragariae method Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Resistance to Mycosphaerella fragariae method Observe the truss location relative to the foliage canopy and record it. Truss exposure 0-1 method Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Ease of calyx removal method Flower date last FLDATE2 Julian date of last flowering for an individual, whole plant, or population. Flower date last Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Ferric reducing antioxidant power trolox method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina method Appearance of first color on fruit Fruit Appearance of first color on fruit. first color appearance FRCOL1 % susceptibility to Mycosphaerella fragariae leaf spot The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. Plant SMYFR Plant susceptibility to Mycosphaerella fragariae leaf spot Fruit yield 1-4 method Estimate fruit yield and record it. The total number of daughter plantlets per plant harvested on a single day at a specific location. Runner daughters Runner daughters DAU Observe the truss location relative to the foliage canopy and record it. Truss exposure 1-5 method Fruit total phenolic acids content Fruit total phenolic acids content The total phenolic acids of fruit. TPHE Plant resistance to Macrophomina phaseolina charcoal rot The plant resistance to charcoal rot caused by Macrophomina phaseolina. resistance to Macrophomina phaseolina charcoal rot RMaPh Plant Weekly flower evaluation The weekly development of flowers. FLEVAL development Flower Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. SSC:TA method Total antioxidant content of leaves. LFTOTANTIOX total antioxidant content Leaf Leaf total antioxidant content Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape UPOV method 1-5 RESIST scale susceptibility to Diplocarpon earliana Plant susceptibility to leaf scorch caused by Diplocarpon earliana Plant The plant susceptibility to leaf scorch caused by Diplocarpon earliana SDE Fruit The color of fruit flesh. FRFLCOL flesh color Fruit flesh color DNA/std DNA_IBL Plant height method Measure the tallest trifoliate leaf from crown to base of leaflets. Fruit yield total weight method Weigh total fruit yield and record it. Plant RDE Plant resistance to Diplocarpon earlianum leaf scorch The plant resistance to leaf scorch caused by Diplocarpon earliana. resistance to Diplocarpon earlianum leaf scorch ease of removal Ease of calyx removal. Calyx ease of removal Calyx CALYXREMOVE Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae % method 1-9 FRYIELD Ordinal scale Crop estimate method Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Number of runners 1-7 method Observe and rate the number of runners per plant. 1-9 FRFLCOL scale Plant susceptibility to Phytophthora fragariae red stele root rot Plant susceptibility to Phytophthora fragariae red stele root rot SPF The plant susceptibility to red stele root rot caused by Phytophthora fragariae. Skin color 1-9 method Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. 1-9 FRFLAV scale Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR 1-8 method mm FRpH resistance to Meloidogyne hapla root knot nematode The plant resistance to root knot nematode Meloidogyne hapla. Plant RMH Plant resistance to Meloidogyne hapla root knot nematode Fruit FRFIRM flesh firmness Fruit flesh firmness The firmness of fruit flesh. Daughter plantlet count Count the total number of daughter plants from a single plant harvested on a single day. resistance to Colletotrichum acutatum anthracnose fruit rot Fruit resistance to Colletotrichum acutatum anthracnose fruit rot RAFR The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. Fruit Plant flower development Growing degree days to first flowering. Plant flower development ECOGDDREQ FLPETALNUM petal number Flower Flower petal number Average flower petal number. Resistance to Verticillium dahliae scale 1-9 Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms development Number of days from flowering to ripe fruit. Fruit FRDEV Fruit development Fruit yield 1-9 O method Estimate fruit yield and record it. Resistance to Phytophthora fragariae % method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. EVERBERIN Whether or not the plant has an everbearing habit Everbearing Plant bearing type Fruit Total anthocyanins total anthocyanin content TACY The total anthocyanins of fruit. Observe where the pedicle breaks during harvest. Pedicle breakage method flavor Fruit FRFLAV Fruit flavor The flavor of fruit. The pH of fruit. Fruit pH Fruit pH FRpH RPCCR Plant resistance to Phytophthora cactorum crown rot The plant resistance to crown rot caused by Phytophthora cactorum. Plant resistance to Phytophthora cactorum crown rot FLDATE100 date The presence of powdery mildew on fruit. PPMF presence of powdery mildew Fruit Fruit presence of powdery mildew FRFRESHWT Fruit fresh weight fresh weight Fruit The weight of fresh fruit. harvest date HARVDATE Record the Julian date when first fruit ripens at a specific location. Fruit harvest date Fruit PEDICLEBREAK Pedicle Pedicle breaking habit breaking habit Pedicle breakage relative to the calyx. Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Titratable acidity citric acid method Fruit Percent weight lost when frozen berries are thawed. drip loss DRIP LOSS Fruit drip loss Plant resistance to Pratylenchus penetrans root lesion nematode Plant resistance to Pratylenchus penetrans root lesion nematode RPP The plant resistance to root lesion nematode Pratylenchus penetrans. ferric reducing antioxidant power FRAP Fruit ferric reducing antioxidant power Fruit Ferric reducing antioxidant power of fruit. Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-3 method diameter The diameter of flower at fully open. FLDIA Flower diameter Flower 0-1 EVER scale 1-9 SUSCEP scale set Fruit set FRUITSET The effeciency of fruit set at a specific location. Fruit Plant Indication of resistance to Botrytis The presence of grey mold caused by Botrytis cinerea on the plant or fruit. BOTRYTIS presence of grey mold Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-9 method DNA/std DNA_ILF Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Resistance to Diplocarpon earlianum method Epipodium measurement method Measure the length of the epipodium of the primary fruit from the calyx to the truss. Stamen presence Stamen presence STAMENPRES The presence of stamens. 0-1 CALPLUG scale BDF Flower Julian date of first open flowers for an individual, whole plant, or population. Bloom date first date first FRLEN Fruit Length of fruit from calyx to tip. length Fruit length 1-9 STAMPRES scale Number of runners 1-9 method Observe and rate the number of runners per plant. Record the number of days from first flowering to ripe fruit. Fruit development method RVD The plant resistance to verticillium wilt caused by Verticillium dahliae. Plant Plant resistance to Verticillium dahliae wilt resistance to Verticillium dahliae wilt harvest date Harvest date HD Record the Julian date when first fruit ripens at a specific location. Fruit PEDICELLEN Stem length of primary fruit from calyx to truss. length Pedicle Pedicle length Flower diameter UPOV method Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Plant The plant resistance to necrosis caused by Neopestalotiopsis sp. Ferric reducing antioxidant power FSH method Plant resistance to necrosis caused by Neopestalotiopsis sp. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). resistance to necrosis caused by Neopestalotiopsis sp. RNEOP Runner total length RTL length total The total length of runners from a single plant harvested on a single day at a specific location. Runner Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. 1-3 CALYXPOS UPOV scale Depth of fruit flesh color Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. Fruit Fruit total water soluble solid content total water soluble solid content SSC The soluble solids content of fruit. cm Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Skin color chromameter reflect chroma index method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. count Observe the presence of flowers during each week in July, August and September. Everbearing flowering method Weekly flowering evaluation Observe the plant weekly and record if it is flowering. average Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina % method x Epipodium length Epipodium Stem length of primary fruit from calyx to truss. length EPILGT 0-7 TRUSS scale 1-3 RESIST scale Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Titratable acidity NaOH method First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface Susceptibility to Podosphaera aphanis method Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. TRUSSSIZ Number of flowers per truss size Truss size Truss CALYXPOS Calyx position The position of the calyx attachment relative to the fruit shoulder. Calyx position Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae 1-3 method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae 0-5 method Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape RB method Reproductive factor Resistance to Neopestalotiopsis sp. scale 0-6 Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants uniformity of color FRCOLORUNIF Fruit uniformity of color. Fruit Fruit uniformity of color toughness SKINTOUGH Skin toughness Skin The toughness of skin. Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. Resistance to Colletotrichum acutatum anthracnose fruit rot % method Fruit total anthocyanin content The total anthocyanins of fruit. ATCYN Fruit total anthocyanin content grams Achene ACHENEPOS Achene position position The postion of achene relative to the fruit surface. Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Fruit flesh color method Chroma Index Luminance 1-4 ACHENEPOS UPOV scale Resistance to Phytophthora cactorum CR 1-5 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. 1-9 FRGLOSS scale Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. Percentage of viable achenes method 1-3 FRCOLUNI scale Peduncle length PEDUNCLEN The length of peduncle. Peduncle length The position of the truss relative to the foliage. Truss exposure Truss exposure TREXPO Observe the number of fruit that a single plant sets in a specific location. Fruit set method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Pg-3-gluc method Plant susceptibility to Phomopsis obscurans leaf blight The plant susceptibility to leaf blight caused by Phomopsis obscurans. susceptibility to Phomopsis obscurans leaf blight SPO Plant Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-4 method 0-6 RESIST scale Observe plant vigor of the two clones and record the average. Plant vigor method Fruit fresh weight method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit shape Fruit shape The shape of fruit. FRSHAPE Measure the diameter of the flowers at fully open Flower diameter method 1-9 CALYXREFLX scale Plant BEARINGTYPE Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. Plant bearing type bearing type Fruit shape uniformity FRSHAPEUNIF Fruit Fruit shape uniformity. shape uniformity Fruit shape GG method Observe and rate average fruit shape on 5 fruit per genotype. Skin toughness method Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. color Achene color ACHENECOL The color of achene on the sun side of a fully ripe fruit. Achene 0-1 BOTRYITIS scale Plant fruit development fruit development ECOGDDREQH Growing degree days to first harvest. Plant Folin-Ciocalteu method Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Calyx plug method Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant SCG The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. 0-5 SUSCEP scale Calyx position 1-3 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Fruit flesh depth of color Depth of fruit flesh color. FRFLCOLDEPTH Fruit flesh depth of color 1-5 PEDUNCLEN scale date Plant RVD Plant resistance to Verticillium dahliae wilt The plant resistance to verticillium wilt caused by Verticillium dahliae. resistance to Verticillium dahliae 1-9 FRSHAPUNI GRIN scale Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Resistance to Meloidogyne hapla method 1-9 FRUITSET scale 0-1 STAMPRES scale DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. 1-7 RUNNERNUM scale 1-3 ACHENEPOS scale Color uniformity method Observe fruit color uniformity from even or very slighly unevento strongly uneven. The plant resistance to leaf blight caused by Phomopsis obscurans. RPO Plant resistance to Phomopsis obscurans leaf blight resistance to Phomopsis obscurans leaf blight Observe the number of flowers per inflorescence. Number of flowers per truss estimation Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. FLDATE2 date 1-10 SKINCOL UPOV scale The degree of erectness of the leaves. habit PLANTHABIT Plant Plant habit 1-9 STAMENHGHT scale Observe and rate the fruit skin color. Skin color 1-10 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR % method Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight 1 method Skin color chromameter reflect L* brightness method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. date first relative Flower Flower date first relative Relative date of first open flowers for an individual, whole plant, or population. FLDATE1REL Observe the plant and record the date when at peak flowering. Flower date 100% method 1-9 PLHAB scale Observe and rate the height of the stamens relative to the receptacle at flower opening. Stamen height method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Leaf pubescence image capturing method Plant Plant ploidy The number of sets of chromosomes in the nucleus. ploidy PLOIDY Fruit firmness penetrometer method Measure fruit firmness in grams using a hand-held penetrometer with a piston. R-S category Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Fruit flavor method Presence of stamens 1-9 method Observe presence of anthers and record it. Peduncle length method Observe average peduncle length and record it. DNARATIO DNA ratio Ratio of sample DNA to standard DNA. Plant DNA ratio Plant Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Leaf total antioxidant assay method Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Susceptibility to Verticillium dahliae 1-5 method The glossiness of skin. SKINGLOSS gloss Skin gloss Skin The height of the stamens relative to the receptacles. STAMENHGHT Stamen height Stamen height Fruit appearance method Observe fruit appearance from not uniform to completely uniform. 1-9 RESIST scale Ratio The plant resistance to powdery mildew caused by Podosphaera aphanis. Plant Plant resistance to Podosphaera aphanis powdery mildew resistance to Podosphaera aphanis powdery mildew RPOAP Estimate fruit yield and record it. Fruit yield 1-9 method size Calyx size The size of the calyx. Calyx CALYXSZ Observe the plant and record the reflexion of the calyx relative to the fruit shoulders Calyx reflexion method TITA Fruit titratable acidity Fruit titratable acidity The titratable acidity content of fruit. Resistance to Podosphaera aphanis % method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. ACHENEVIABLE The percentage of achenes that are viable. Percentage of viable achenes Achene viable 1-3 ACHENECOL UPOV scale Meq/100g citric acid Stembreak txt Susceptibility to Phomopsis obscurans method Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Pratylenchus penetrans number of nematodes/g root method Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. 1-3 PLHAB scale 1-5 RMH scale 1-8 RESIST scale Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Flower date first relative method Observe the plant and record the relative flowering of the first open flowers. Presence or absence of central plug after calyx removal. CALYXPLUG Calyx plug Calyx plug meters Meq NaOH/100g mg/L Cy-3-gluc Eq Plant resistance to Phytophthora cactorum root rot resistance to Phytophthora cactorum root rot Plant RPCRR The plant resistance to root rot caused by Phytophthora cactorum. Observe the plant and assess the erectness or spreading of the leaves along the ground. Plant habit scale method Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. Harvest and record the largest fruit over the harvest season. Fruit fresh weight largest method First runner date Runner The Julian date of appearance of the first runners. RUNNERDATE first emergence 1-9 FRSHAPE UPOV scale days Presence of powdery mildew on fruit method Observe the presence of powdery mildew on ripe fruit. Flower date first method Observe the plant and record the date the first flowers open. Presence of stamens 0-1 method Observe presence of anthers and record it. date first Flower date first FLDATE1 Flower Julian date of first open flowers for an individual, whole plant, or population. 1-9 PLANTVIGOR scale Count the number of petals on 10 flowers and average the results Petal number average method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. Fruit pH method Perpetual flowering method Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-5 method FLDATE1 Date 1-5 CALYXPOS scale Fruit ripening trait Fruit ripening trait FRRP The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA The titratable acidity content of fruit. Fruit TITA Fruit titratable acidity titratable acidity 1-9 CALYXREM scale susceptibility to Podosphaera aphanis The plant susceptibility to powdery mildew caused by Podosphaera aphanis. SPOAP Plant Plant susceptibility to Podosphaera aphanis powdery mildew Susceptibility to Colletotrichum gloeosporioides weekly method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. Calyx size method 1-5 TREXPOS UPOV scale Number of runners count method Harvest and count the number of runners collected from a single crown on a single day in a specific location. Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method The density of trichomes on the leaf surface. Leaf LFPUBESC pubescence Leaf pubescence Plant resistance to Phytophthora fragariae red stele root rot The plant resistance to red stele root rot caused by Phytophthora fragariae. Plant RPF resistance to Phytophthora fragariae red stele root rot Plant resistance to Mycosphaerella fragariae leaf spot resistance to Mycosphaerella fragariae leaf spot RMYFR The plant resistance to Mycosphaerella fragariae. Plant 1-3 pubescence scale Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae % method Calyx Reflexion of the calyx relative to the fruit shoulders. CALYXREFLX Calyx reflexion reflexion Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Susceptibility to Diplocarpon earlianum method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. Titratable acidity 0.5 g citric acid method SKINCOL Skin color on the sun side of a fully ripe fruit. Skin color color Skin 1-9 FLDATE1REL UPOV scale 1-9 SKINTOUGH scale Record the Julian date when first fruit ripens at a specific location. Harvest date method Drip loss method Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Skin color SKINCOL Skin color on the sun side of a fully ripe fruit. Skin color DNA ration determination using DAPI with internal standard = iceberg lettuce method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Pedicel measurement method Measure the length of the pedicel of the primary fruit from the calyx to the truss. The number of runners. number Runner RUNNERNUM Runner number Achene position 1-3 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Fruit fresh weight 5L method Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. method_of 1-9 FRYIELD scale breaking habit Pedicle breakage relative to the calyx. Pedicle STEMBREAK Stem breaking 1-3 CALYXREFLX UPOV scale GG scale Susceptibility to Phytophthora fragariae method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. variable_of 1-9 FRFIRM scale Record the number of weeks of flowering after week 11 from the first week of bloom. Perpetual flowering RB method Soluble solids method Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. 1-7 PEDBRK scale FSH EQ μmoles/g fruit mg/L Pg-3-gluc kg−1 Plant height height Plant Height of tallest trifoliate leaf. PLANTHT 1-9 FLDIA UPOV scale 1-3 CALYXSZ scale g/100g vigor Plant vigor PLANTVIGOR Plant The vigor of plant. Fruit ripen days development Fruit FRD Number of days from flowering to ripe fruit. 1-9 RUNNERNUM scale 0-1 FLEVAL scale Measure the length of the fruit from the calyx to the tip and record in mm. Fruit length method 1-9 FRSHAPE RB scale First runner date method Observe the plant and record the Julian date the first runners emerge at a specific location. FLDATE100 date 100% Flower Julian date of peak flowering for an individual, whole plant, or population. Flower date 100% Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis 1-9 method Growing degree days harvest method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. 1-5 SUSCEP scale 1-4 FRYIELD scale Flower date last method Observe the plant and record the date when at the end of flowering. scale_of mg/L Gallic Acid EQ 1-9 ACHENECOL scale Fruit pH 2 method Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Cy-3-gluc method Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Fruit firmness method Trolox EQ μmoles/g FW 1-9 SKINCOL scale 1-5 FRCOLUNI scale