UPOV FLDIA_FLDIAM_cm FRYIELD_FRYIELDTWM_g USDA USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg University of California, Davis Pincot et al. 2020 RVD_RVD1-9M_1-9RESISTScale Feldmann, et al. SVD_SVD1-5M_1-5SUSCEPscale UC Davis 6.0 medium to large GDR RosBREED HARVDATE_HARVDATEM_date PEDICELLEN_PEDICELLENM_cm USDA few 3 4 globose GDR RosBREED ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq FRDEV_FRDEVM_days USDA Biswas et al. 2019 University of Delaware LFPUBESC_LFPUBESCM_1-3LFPUBESCscale PLANTHT_PLANTHTM_cm USDA 2 slightly uneven USDA FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale 1 upright GDR FRpH_pHM_pH RosBREED BEARINGTYPE_BEARINGRBM_count GDR RosBREED RDE_REDM_1-9RESISTscale USDA USDA RPOAP_RPOAP%M_% GDR FRFRESHWT_FRFRESHWT5M_g RosBREED RosBREED GDR FLEVAL_FLEVALM_0-1FLEVALscale pedicle breaks just above calyx 5 RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale CPBR USDA FRRP_FRRPM_Ratio hundreds 9 9 very light brown or green GDR TREXPOS_TREXPOS0-1M_0-1TREXPOSscale RosBREED FLDATE2_FLDATE2M_date USDA USDA FRYIELD_FRYIELD1-4M_1-4FRYIELDscale 9 very easily removed GDR CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale RosBREED CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale UPOV GDR RosBREED ECOGDDREQ_GDDFM_days RosBREED SMYFR_SMYFRM_1-9SUSCEPscale GDR 2 yellow does not remove 1 stem does not break, berry separates from calyx easily N USDA STEMBREAK_PEDBRKM_text TotalPhenolics_F-CM_mg/L_GallicAcidEQ GDR RosBREED DRIPLOSS_DRIPLOSSM_g RosBREED GDR 7 pedicle breaks well above calyx USDA DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL conic 1 USDA RUNNERDATE_FRUNNERM_date UPOV FLDIA_FLDIAM_1-9FLDIAUPOVscale calyx size smaller than fruit diameter 1 2 conic RosBREED ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale GDR DAU_RUNDAUM_count USDA medium 5 FLDATE1_FLDATE1M_FLDATE1date USDA dark red 9 globose-conic 5 GDR RosBREED STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale oblong 5.0 FRRAFR_FRAFR%M_% University of Florida Salinas et al. 2019 divides right before fruit 5.0 R resistant not reflexed 1 UC Davis RFOF_RFOF1-5M_1-5RESISTscale Pincot, et al. very sweet, no off-flavors 9 ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale GDR RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale RosBREED GDR GDR RosBREED CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale highly susceptible/severe symptoms 9 9 extremely vigorous USDA TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq dead 3.0 oblate 7.0 large 7.0 RMaPh_ RMaPhM_1-5RESISTscale Knapp, et al. UC Davis 2 yellowish white 1 high yield GDR RosBREED SPOAP_SPOAPM_1-9SUSCEPscale USDA RPOAP_RPOAP1-9M_1-9RESISTscale RosBREED GDR SKINCOL_SKINCOL1-9M_1-9SKINCOLscale 9.0 dark red 1 very small low 3 USDA PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale USDA FRLEN_FRLENM_mm CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale USDA PLANTHABIT_PLHABM_1-9PLHABscale USDA RosBREED FRFLCOL_FRFLCOLM_1-9FRFLCOLscale GDR 1 no fruit 2 semi-upright USDA RPF_RPF%M_% Capocasa et al. 2008 Università Politecnica delle Marche SKINCOL_REFLECTLM_L* CALYXSZ_CALYXSZM_1-3CALYXSZscale GDR RosBREED very firm 9 USDA RTL_RUNTOTLENM_m SKINCOL_REFLECTCIXM_ChromaIndex Capocasa et al. 2008 Università Politecnica delle Marche EVERBERIN_EVER0-1M_0-1EVERscale USDA RosBREED FRFLAV_FRFLAVM_1-9FRFLAVscale GDR PPMF_PPMFM_0-1PMFscale GDR RosBREED inserted 1 TITA_TITAM_TITAMeqNAOH/100g Università Politecnica delle Marche Capocasa et al. 2008 low yield 3 slightly symptomatic 1.5 RosBREED GDR SKINGLOSS_SKINGLOSSM_1-9GLOSSscale GDR RosBREED ECOGDDREQH_GDDHM_days 3 early high 7 even or very slightly uneven 1 FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale GDR RosBREED raised 3 RosBREED PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale GDR BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale UPOV downwards 3 GDR SDE_SDEM_1-9SUSCEPscale RosBREED USDA Zurn, et al. RMaPh_ RMaPh%M_ % Capocasa et al. 2008 Università Politecnica delle Marche FRFIRM_PENEM_g TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale UPOV 4 slightly above USDA FRD_FRRIPEM_days BOTRYTIS_RBCM_0-1BOTRYTISscale USDA PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale GDR RosBREED FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale USDA same level 3 6.0 rhombic 4 ovate divides close to crown 1 1 not remontant 1 highly resistant/no symptoms light orange 3 9.0 very large GDR RosBREED FRFLCOLDEPTH_FRCOLDEPTHM_% 8.0 circular FLDATE100_FLDATE100M_FLDATE100date USDA fingerling 6.0 USDA RPCCR_RPCCR%M_% RUNNERNUM_RUNNERNUMCT_count USDA RMH_RMHM_1-5RMHscale USDA Pinkerton & Finn RPP_RPPM_Rf NEM-RF_RPPM_Rf USDA Pinkerton & Finn USDA LFRW_FRFRESHWLGM_g USDA BLDATE1_FLDATE1M_FLDATE1date plant has stamens 9 slightly uneven 3 GDR SSC_SSCM_SSCg/100g RosBREED 1 high density white 1 UPOV SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale highly resistant/no symptoms 9 globose-conic 5.0 reniform 1 1 sunken RPCCR_RPCCR1-5M_1-5RESISTscale UC Davis Jiménez, et al. USDA RMYFR_RMYFRM_% completely uniform/symmetrical and attractive 9 USDA 5FRW_FRFRESHWT5LM_g oblate 9.0 5.0 strongly above USDA FLPETALNUM_FLPETALNUMM_average RosBREED TITA_TITAM_TITAMeq/100gCA GDR UPOV TRUSSSIZ_TRUSSM_1-9scale Eikemo, et al. NCRI RPCCR_RPCCR1-8M_1-8RESISTscale 1 highly susceptible/severe symptoms 3 strongly uneven strongly below 1 LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW Biswas et al. 2019 University of Delaware Capocasa et al. 2008 FRAP_FRAPFSHM_FSHEQumoles/g fruit Università Politecnica delle Marche UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale RNEOP_RNEOP0-6M_0-6RESISTscale University of North Carolina Fernandez et al. calyx size equal to fruit diameter 2 3 cordate outwards 2 GDR RosBREED ACHENEVIABLE_ACHVIAM_% USDA PLOIDY_FCDAPIICFM_x 9 tough medium density 2 USDA STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA 3 spreading Università Politecnica delle Marche Capocasa et al. 2008 FRAP_FRAPTROM_TroloxEQumoles/g FW TITA_TITA0.5M_TITAMeq/100gCA USDA GDR RosBREED SPO_SPOM_1-9SUSCEPscale medium 5 greenish 1 USDA RPCRR_RPC%M_% no flowers 1 RosBREED GDR CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV medium red 8.0 FRUITSET_FRSETM_1-9FRUITSETscale USDA FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale UPOV 3 small RFOF_RFOF%M_% USDA Zurn, et al. DNARATIO_DNADAPIICFM_DNA/stdDNAILF USDA FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale CPBR 1 long conic GDR RosBREED SPF_SPFM_0-5SUSCEPscale medium orange 4 USDA FRCOL1_FRCOL1M_date breakage is inconsistent 3 RVD_RVD1-3M_1-3RESISTscale Vining, et al. Oregon State GDR TRUSSSIZ_TRUSSCOUNTM_ave RosBREED GDR RosBREED RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale large to very large 8.0 attribute Strawberry ontology 4 divides 75% from crown 2 slightly below CE_CROPEST_g USDA 9.0 very high FRpH_pH2M_pH USDA 1 stamens shorter than receptacle level with fruit 2 FRSHAPE_FRSHAPEGGM_TXT USDA very late 9.0 above surface 4 orange red 5.0 USDA EPILGT_EPILGTM_cm 1 highly resistant/no symptoms 3 red USDA HD_FIRSTHARVM_date small to medium 4 GDR RPF_RPF0-5M_0-5RESISTscale RosBREED 1 dull even 1 highly resistant/no symptoms 1 7 late blackish red 10.0 CPBR PLANTHABIT_PLHABM_1-3PLHABscale 1 no fruit 6.0 pink spreading 1 1 white entity very small to small 2 many 7 RosBREED FRYIELD_FRYIELD1-9M_1-9FRYIELDscale GDR medium yield 2 stamens taller than receptacle 9 soft 1 University of Florida Chandra et al. 2021 SCG_SCGWKM_R-Scategory 7 many flowers very resistant, healthy 1.0 9.0 wedged 1 very soft divides 50% from crown 3 erect 9 plant has no stamens 1 GDR RosBREED FRFIRM_FRFIRMM_1-9FRFIRMscale medium number of flowers 5 2 divides at 25% from crown 2 partially remontant V variable, no consistent breaking habit 9 highly resistant/no symptoms UPOV FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale 1 pinkish white 2 long conic Y Stem breaks just above calyx, good for fresh market FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale UPOV 3 protruding 5 calyx attachment is sunken below shoulder USDA PLOIDY_FCDAPIIBLM_x 8 highly susceptible/severe symptoms USDA RVD_RVD%_% mildly symptomatic 2.0 globose 3 very early 1 susceptible S stem breaks well above calyx Y** 9 complete fruit set acronym 9 blackish red not sweet, bad off-flavors 1 not uniform/malformed 1 1 dark STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale USDA USDA RPO_RPOM_% strongly below surface 1 1 upwards level with surface 3 1 pedicle does not break CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale UPOV 3 low density even 2 3 few flowers no runners 1 fully reflexed 9 4 no fruit set 9 overcropped cordiform 7 highly susceptible/severe symptoms 1 1 no fruit set very glossy 9 day neutral 4 highly susceptible/severe symptoms 5 dead 1 absent or very weak 1 1 calyx attachment raised above shoulder (necked) very symptomatic 2.5 medium 5.0 5 intermediate 7.0 light red 3 calyx size larger than fruit diameter fully remontant 3 2 slightly below surface strongly uneven 5 conic-wedge 3 calyx attachment is even with fruit 3 Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Fruit flavor method count Runner total length method Harvest all runners on a single day and measure the total length. Harvest date method Record the Julian date when first fruit ripens at a specific location. Plant fruit development Plant fruit development Growing degree days to first harvest. ECOGDDREQH 1-9 FLDIA UPOV scale development Flower Weekly flower evaluation The weekly development of flowers. FLEVAL Achene position 1-3 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. Depth of fruit flesh color pH Fruit The pH of fruit. Fruit pH FRpH Fruit development method Record the number of days from first flowering to ripe fruit. Crop estimate Plant CE fruit yield The amount of harvested fruit per plant per season. Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days harvest method RPP resistance to Pratylenchus penetrans root lesion nematode Plant resistance to Pratylenchus penetrans root lesion nematode The plant resistance to root lesion nematode Pratylenchus penetrans. Plant Flower FLDIA diameter The diameter of flower at fully open. Flower diameter g/100g date Estimate fruit yield and record it. Fruit yield 1-9 O method % Pedicle length Stem length of primary fruit from calyx to truss. PEDICELLEN Pedicle length 1-5 RESIST scale The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. susceptibility to Mycosphaerella fragariae leaf spot Plant susceptibility to Mycosphaerella fragariae leaf spot SMYFR Plant Daughter plantlet count Count the total number of daughter plants from a single plant harvested on a single day. Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Susceptibility to Verticillium dahliae 1-5 method Plant resistance to Verticillium dahliae wilt The plant resistance to verticillium wilt caused by Verticillium dahliae. RVD Plant resistance to Verticillium dahliae wilt mg/L Cy-3-gluc Eq RPO Plant resistance to Phomopsis obscurans leaf blight resistance to Phomopsis obscurans leaf blight The plant resistance to leaf blight caused by Phomopsis obscurans. 1-4 FRYIELD scale Calyx ease of removal CALYXREMOVE Ease of calyx removal. ease of removal Calyx Titratable acidity NaOH method Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Flower diameter method Measure the diameter of the flowers at fully open Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR % method Pedicle breakage relative to the calyx. breaking habit Pedicle STEMBREAK Stem breaking 1-9 FRFLCOL scale Pedicle breakage relative to the calyx. Pedicle breaking habit PEDICLEBREAK breaking habit Pedicle habit The degree of erectness of the leaves. PLANTHABIT Plant Plant habit 0-1 CALPLUG scale resistance to Phytophthora cactorum crown rot Plant Plant resistance to Phytophthora cactorum crown rot The plant resistance to crown rot caused by Phytophthora cactorum. RPCCR flesh depth of color Fruit FRFLCOLDEPTH Depth of fruit flesh color. Fruit flesh depth of color The total phenolic acids of fruit. Fruit total phenolic acids content total phenolic acids content Fruit TPHE Fruit fresh weight method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. Plant resistance to Meloidogyne hapla root knot nematode The plant resistance to root knot nematode Meloidogyne hapla. Plant resistance to Meloidogyne hapla root knot nematode RMH susceptibility to Phytophthora fragariae red stele root rot SPF Plant The plant susceptibility to red stele root rot caused by Phytophthora fragariae. Plant susceptibility to Phytophthora fragariae red stele root rot Fruit flesh color Fruit flesh color The color of fruit flesh. FRFLCOL resistance to Colletotrichum acutatum anthracnose fruit rot Fruit resistance to Colletotrichum acutatum anthracnose fruit rot Fruit RAFR The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. position Achene position ACHENEPOS The postion of achene relative to the fruit surface. Achene Harvest and record the largest fruit over the harvest season. Fruit fresh weight largest method Harvest and count the number of runners collected from a single crown on a single day in a specific location. Number of runners count method FRYIELD fruit yield Plant Plant fruit yield The fruit yield of one individual. FLPETALNUM Flower petal number Average flower petal number. Flower petal number Skin color 1-10 method Observe and rate the fruit skin color. Truss Truss exposure The position of the truss relative to the foliage. TREXPO exposure Skin toughness SKINTOUGH The toughness of skin. toughness Skin average Meq/100g citric acid Epipodium length length Stem length of primary fruit from calyx to truss. Epipodium EPILGT mm ATCYN The total anthocyanins of fruit. Fruit total anthocyanin content total anthocyanin content Fruit Resistance to Diplocarpon earlianum method Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Susceptibility to Phomopsis obscurans method Fruit shape UPOV method Observe and rate average fruit shape on 3-5 fruit per genotype. Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Crop estimate method Skin color SKINCOL Skin color on the sun side of a fully ripe fruit. color Skin FRUITSET Fruit set set The effeciency of fruit set at a specific location. Fruit Plant The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides SCG The shape of fruit. Fruit shape FRSHAPE shape Fruit 1-3 CALYXSZ scale Fruit harvest date HD Record the Julian date when first fruit ripens at a specific location. Harvest date Stamen STAMENHGHT Stamen height height The height of the stamens relative to the receptacles. Record the number of weeks of flowering after week 11 from the first week of bloom. Perpetual flowering RB method Bloom date first Julian date of first open flowers for an individual, whole plant, or population. Flower date first BDF SDE Plant Plant susceptibility to leaf scorch caused by Diplocarpon earliana susceptibility to Diplocarpon earliana The plant susceptibility to leaf scorch caused by Diplocarpon earliana Skin color 1-9 method Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. size TRUSSSIZ Truss size Number of flowers per truss Truss Plant susceptibility to Phomopsis obscurans leaf blight SPO Plant The plant susceptibility to leaf blight caused by Phomopsis obscurans. susceptibility to Phomopsis obscurans leaf blight 1-3 CALYXREFLX UPOV scale Meq NaOH/100g first emergence The Julian date of appearance of the first runners. RUNNERDATE First runner date Runner length The length of peduncle. Peduncle Peduncle length PEDUNCLEN Chroma Index Susceptibility to Mycosphaerella fragariae method Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Ferric reducing antioxidant power trolox method Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Percentage of viable achenes method Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. TITA titratable acidity Fruit The titratable acidity content of fruit. Fruit titratable acidity Total antioxidant content of leaves. total antioxidant content LFTOTANTIOX Leaf Leaf total antioxidant content Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum RR% method Leaf total antioxidant assay method Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. FLDATE1 Date Fruit uniformity of color Fruit Fruit uniformity of color. uniformity of color FRCOLORUNIF Resistance to Phytophthora cactorum CR 1-5 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. color Skin SKINCOL Skin color Skin color on the sun side of a fully ripe fruit. Plant resistance to Macrophomina phaseolina charcoal rot The plant resistance to charcoal rot caused by Macrophomina phaseolina. RMaPh Plant resistance to Macrophomina phaseolina charcoal rot Fruit total water soluble solid content SSC Fruit total water soluble solid content The soluble solids content of fruit. 1-3 ACHENECOL UPOV scale 1-3 pubescence scale Petal number average method Count the number of petals on 10 flowers and average the results Skin color chromameter reflect L* brightness method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. Observe the presence of flowers during each week in July, August and September. Everbearing flowering method Luminance FRDEV Number of days from flowering to ripe fruit. Fruit development development Fruit Observe the truss location relative to the foliage canopy and record it. Truss exposure 1-5 method FLDATE2 date 1-9 RESIST scale 1-9 CALYXREM scale Total anthocyanins Pg-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. 1-5 RMH scale The glossiness of skin. Skin SKINGLOSS gloss Skin gloss Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. Susceptibility to Podosphaera aphanis method 1-9 SKINCOL scale Fruit shape RB method Observe and rate average fruit shape on 3-5 fruit per genotype. Resistance to Podosphaera aphanis 1-9 method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. 1-3 PLHAB scale Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Folin-Ciocalteu method mg/L Gallic Acid EQ Resistance to Podosphaera aphanis % method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Appearance of first color on fruit. Appearance of first color on fruit Fruit FRCOL1 first color appearance Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Leaf pubescence image capturing method Plant vigor method Observe plant vigor of the two clones and record the average. x Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method Calyx The size of the calyx. size CALYXSZ Calyx size Julian date of last flowering for an individual, whole plant, or population. date last FLDATE2 Flower Flower date last BOTRYTIS The presence of grey mold caused by Botrytis cinerea on the plant or fruit. Indication of resistance to Botrytis Plant presence of grey mold Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Flower diameter UPOV method Achene position 1-4 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Measure the length of the epipodium of the primary fruit from the calyx to the truss. Epipodium measurement method RPCRR resistance to Phytophthora cactorum root rot Plant The plant resistance to root rot caused by Phytophthora cactorum. Plant resistance to Phytophthora cactorum root rot The number of sets of chromosomes in the nucleus. Plant PLOIDY ploidy Plant ploidy Skin color chromameter reflect chroma index method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. 1-9 RUNNERNUM scale 1-3 CALYXPOS UPOV scale date first relative Flower FLDATE1REL Flower date first relative Relative date of first open flowers for an individual, whole plant, or population. Flower date first Julian date of first open flowers for an individual, whole plant, or population. Flower FLDATE1 date first resistance to Fusarium oxysporum f. sp. fragariae wilt The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. RFOF Plant resistance to Fusarium oxysporum f. sp. fragariae wilt Plant Measure fruit firmness in grams using a hand-held penetrometer with a piston. Fruit firmness penetrometer method Observe and rate the height of the stamens relative to the receptacle at flower opening. Stamen height method Resistance to Phytophthora fragariae 0-5 method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. LFPUBESC Leaf Leaf pubescence The density of trichomes on the leaf surface. pubescence Plant Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). Plant resistance to necrosis caused by Neopestalotiopsis sp. resistance to necrosis caused by Neopestalotiopsis sp. Ferric reducing antioxidant power FSH method The plant resistance to necrosis caused by Neopestalotiopsis sp. RNEOP Fruit yield 1-4 method Estimate fruit yield and record it. Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae % method cm days Fruit yield total weight method Weigh total fruit yield and record it. grams Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. SSC:TA method Peduncle length method Observe average peduncle length and record it. Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Fruit pH 2 method Fruit firmness method Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Measure the length of the fruit from the calyx to the tip and record in mm. Fruit length method Ease of calyx removal method Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Drip loss method g 1-9 FRSHAPUNI GRIN scale The plant susceptibility to verticillium wilt caused by Verticillium dahliae. susceptibility to Verticillium dahliae wilt Plant SVD Plant susceptibility to Verticillium dahliae wilt Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Resistance to Mycosphaerella fragariae method The color of achene on the sun side of a fully ripe fruit. ACHENECOL Achene color color Achene Achene color 1-9 method Observe the average achene color on 3-5 fruit per genotype and record it. 5FRW fresh weight Fruit The weight of fresh fruit. Fruit weight 1-9 CALYXREFLX scale Observe and rate average fruit shape on 5 fruit per genotype. Fruit shape GG method flavor FRFLAV Fruit Fruit flavor The flavor of fruit. Fruit FRAP ferric reducing antioxidant power Fruit ferric reducing antioxidant power Ferric reducing antioxidant power of fruit. Fruit Fruit ripening trait The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA FRRP ripening trait Calyx reflexion method Observe the plant and record the reflexion of the calyx relative to the fruit shoulders 1-9 FRYIELD scale 1-8 RESIST scale Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants Resistance to Neopestalotiopsis sp. scale 0-6 DNA ration determination using DAPI with internal standard = iceberg lettuce method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Resistance to Macrophomina phaseolina method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Achene ACHENEVIABLE Percentage of viable achenes viable The percentage of achenes that are viable. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Fruit harvest date HARVDATE Fruit harvest date Record the Julian date when first fruit ripens at a specific location. RVD Plant Plant resistance to Verticillium dahliae wilt The plant resistance to verticillium wilt caused by Verticillium dahliae. resistance to Verticillium dahliae Plant susceptibility to Podosphaera aphanis powdery mildew The plant susceptibility to powdery mildew caused by Podosphaera aphanis. SPOAP susceptibility to Podosphaera aphanis Plant Length of fruit from calyx to tip. length FRLEN Fruit length Fruit position Calyx The position of the calyx attachment relative to the fruit shoulder. CALYXPOS Calyx position 1-5 PEDUNCLEN scale Susceptibility to Phytophthora fragariae method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. Runner daughters daughters Runner DAU The total number of daughter plantlets per plant harvested on a single day at a specific location. Plant vigor PLANTVIGOR vigor Plant The vigor of plant. Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. Resistance to Colletotrichum acutatum anthracnose fruit rot % method Number of runners 1-7 method Observe and rate the number of runners per plant. Flower date first relative method Observe the plant and record the relative flowering of the first open flowers. Fruit drip loss Fruit drip loss DRIP LOSS Percent weight lost when frozen berries are thawed. Observe the number of fruit that a single plant sets in a specific location. Fruit set method Plant Whether or not the plant has an everbearing habit Everbearing bearing type EVERBERIN Fruit Fruit shape uniformity shape uniformity Fruit shape uniformity. FRSHAPEUNIF meters Resistance to Meloidogyne hapla method Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Truss exposure 0-1 method Observe the truss location relative to the foliage canopy and record it. Fruit appearance method Observe fruit appearance from not uniform to completely uniform. 1-7 RUNNERNUM scale RUNNERNUM number Runner number Runner The number of runners. Julian date of peak flowering for an individual, whole plant, or population. FLDATE100 Flower Flower date 100% date 100% development Fruit Number of days from flowering to ripe fruit. Fruit ripen days FRD 1-5 SUSCEP scale Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Susceptibility to Diplocarpon earlianum method 0-7 TRUSS scale mg/L Pg-3-gluc kg−1 Total anthocyanins Fruit TACY The total anthocyanins of fruit. total anthocyanin content Calyx position 1-5 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Observe where the pedicle breaks during harvest. Pedicle breakage method Calyx CALYXREFLX Calyx reflexion Reflexion of the calyx relative to the fruit shoulders. reflexion FRpH GG scale Resistance to Verticillium dahliae 1-3 method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. 1-9 FRYIELD Ordinal scale Plant habit scale method Observe the plant and assess the erectness or spreading of the leaves along the ground. Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx plug method Trolox EQ μmoles/g FW Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR 1-8 method RDE resistance to Diplocarpon earlianum leaf scorch The plant resistance to leaf scorch caused by Diplocarpon earliana. Plant Plant resistance to Diplocarpon earlianum leaf scorch 0-1 PMF scale Color uniformity method Observe fruit color uniformity from even or very slighly unevento strongly uneven. Observe presence of anthers and record it. Presence of stamens 0-1 method Pedicel measurement method Measure the length of the pedicel of the primary fruit from the calyx to the truss. First runner date method Observe the plant and record the Julian date the first runners emerge at a specific location. Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days flowering method R-S category LFRW fresh weight largest Largest fruit weight Fruit The weight of fresh fruit. Fruit PPMF The presence of powdery mildew on fruit. presence of powdery mildew Fruit presence of powdery mildew 1-10 SKINCOL UPOV scale Stamen presence STAMENPRES Stamen The presence of stamens. presence Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-3 method Fruit flesh color method Observe and rate the average fruit flesh color on 3-5 fruit per genotype. 1-3 ACHENEPOS scale The firmness of fruit flesh. flesh firmness Fruit flesh firmness Fruit FRFIRM 1-3 FRCOLUNI scale 0-5 SUSCEP scale Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae % method Fruit fresh weight 1 method Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Presence of stamens 1-9 method Observe presence of anthers and record it. Stembreak txt Titratable acidity 0.5 g citric acid method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. Plant height Height of tallest trifoliate leaf. PLANTHT Plant height Plant Plant resistance to Podosphaera aphanis powdery mildew RPOAP The plant resistance to powdery mildew caused by Podosphaera aphanis. resistance to Podosphaera aphanis powdery mildew 0-1 FLEVAL scale 1-9 FLDATE1REL UPOV scale Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-3 method 1-5 CALYXPOS scale 1-5 TREXPOS UPOV scale plug Presence or absence of central plug after calyx removal. Calyx CALYXPLUG Calyx plug DNA/std DNA_ILF Observe the number of flowers per inflorescence. Number of flowers per truss estimation Flower date last method Observe the plant and record the date when at the end of flowering. FLDATE100 date Reproductive factor Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Plant flower development Plant flower development Growing degree days to first flowering. ECOGDDREQ Fruit FRFIRM Fruit flesh firmness The firmness of fruit flesh. flesh firmness method_of Plant DNARATIO DNA ratio Ratio of sample DNA to standard DNA. Plant DNA ratio 1-9 PLHAB scale Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Susceptibility to Colletotrichum gloeosporioides weekly method Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method Total anthocyanins Cy-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. length total Runner total length RTL The total length of runners from a single plant harvested on a single day at a specific location. Runner Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Plant resistance to Mycosphaerella fragariae leaf spot The plant resistance to Mycosphaerella fragariae. Plant RMYFR resistance to Mycosphaerella fragariae leaf spot Presence of powdery mildew on fruit method Observe the presence of powdery mildew on ripe fruit. Flower date first method Observe the plant and record the date the first flowers open. Resistance to Verticillium dahliae scale 1-9 Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Plant height method Measure the tallest trifoliate leaf from crown to base of leaflets. DNA/std DNA_IBL Ratio Flower date 100% method Observe the plant and record the date when at peak flowering. Titratable acidity citric acid method Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Fruit pH method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae % method 0-6 RESIST scale Estimate fruit yield and record it. Fruit yield 1-9 method 1-9 FRSHAPE RB scale 0-1 BOTRYITIS scale fresh weight Fruit Fruit fresh weight FRFRESHWT The weight of fresh fruit. 1-9 SUSCEP scale 0-1 EVER scale Weekly flowering evaluation Observe the plant weekly and record if it is flowering. Perpetual flowering method Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. Plant BEARINGTYPE Plant bearing type bearing type Record the Julian date when first fruit develops ripe color on at least half of its surface First fruit color method 1-9 STAMENHGHT scale 1-4 PERPFL UPOV scale DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. 1-3 RESIST scale variable_of 1-9 SKINTOUGH scale Resistance to Macrophomina phaseolina % method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Number of runners 1-9 method Observe and rate the number of runners per plant. 1-9 PLANTVIGOR scale Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. Plant resistance to Phytophthora fragariae red stele root rot The plant resistance to red stele root rot caused by Phytophthora fragariae. Plant RPF resistance to Phytophthora fragariae red stele root rot FSH EQ μmoles/g fruit 0-1 TREXPOS scale Fruit fresh weight 5L method Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. The titratable acidity content of fruit. Fruit titratable acidity Fruit TITA titratable acidity 1-9 FRSHAPE UPOV scale 1-4 ACHENEPOS UPOV scale Soluble solids method Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. 1-9 FRUITSET scale 1-9 FRGLOSS scale 0-1 STAMPRES scale 1-9 FRFIRM scale 1-9 STAMPRES scale 1-5 FRCOLUNI scale Calyx size method Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. 1-9 FRFLAV scale 1-7 PEDBRK scale 1-9 ACHENECOL scale scale_of