UPOV CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale 1 not sweet, bad off-flavors 7.0 oblate FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale UPOV RosBREED FRFLCOLDEPTH_FRCOLDEPTHM_% GDR GDR DRIPLOSS_DRIPLOSSM_g RosBREED GDR ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale RosBREED Università Politecnica delle Marche Capocasa et al. 2008 SKINCOL_REFLECTCIXM_ChromaIndex cordate 3 USDA FRpH_pH2M_pH highly susceptible/severe symptoms 1 TITA_TITAM_TITAMeqNAOH/100g Capocasa et al. 2008 Università Politecnica delle Marche Oregon State Vining, et al. RVD_RVD1-3M_1-3RESISTscale no fruit 1 very small 1 FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg USDA 8 highly susceptible/severe symptoms red 3 USDA FRUITSET_FRSETM_1-9FRUITSETscale GDR SDE_SDEM_1-9SUSCEPscale RosBREED USDA RPCCR_RPCCR%M_% blackish red 10.0 USDA DAU_RUNDAUM_count 1 calyx size smaller than fruit diameter SCG_SCGWKM_R-Scategory Chandra et al. 2021 University of Florida RTL_RUNTOTLENM_m USDA fully remontant 3 GDR PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale RosBREED FRLEN_FRLENM_mm USDA globose 4 STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale USDA 5.0 strongly above RosBREED ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale GDR UPOV FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale SMYFR_SMYFRM_1-9SUSCEPscale RosBREED GDR 7 late dead 1 UPOV CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale GDR STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale RosBREED USDA FRD_FRRIPEM_days FRRP_FRRPM_Ratio USDA 2 medium density 9 highly resistant/no symptoms RosBREED FRFLCOL_FRFLCOLM_1-9FRFLCOLscale GDR spreading 3 dead 3.0 RosBREED GDR HARVDATE_HARVDATEM_date FRYIELD_FRYIELD1-9M_1-9FRYIELDscale RosBREED GDR FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale UPOV 4 day neutral PEDICELLEN_PEDICELLENM_cm USDA breakage is inconsistent 3 USDA RPCRR_RPC%M_% SPF_SPFM_0-5SUSCEPscale RosBREED GDR USDA HD_FIRSTHARVM_date RDE_REDM_1-9RESISTscale USDA long conic 2 EVERBERIN_EVER0-1M_0-1EVERscale USDA STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale USDA very early 1 USDA CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale University of Delaware LFPUBESC_LFPUBESCM_1-3LFPUBESCscale Biswas et al. 2019 2 even stem does not break, berry separates from calyx easily N FLDIA_FLDIAM_cm UPOV 9 very light brown or green RMaPh_ RMaPh%M_ % USDA Zurn, et al. R resistant GDR ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq RosBREED 3 few flowers 9 very sweet, no off-flavors level with fruit 2 yellowish white 2 RosBREED GDR SKINCOL_SKINCOL1-9M_1-9SKINCOLscale Università Politecnica delle Marche Capocasa et al. 2008 SKINCOL_REFLECTLM_L* GDR RosBREED CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA 9.0 very late USDA FRYIELD_FRYIELD1-4M_1-4FRYIELDscale 3 calyx attachment is even with fruit FRRAFR_FRAFR%M_% University of Florida Salinas et al. 2019 GDR RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale RosBREED RPF_RPF%M_% USDA 1 plant has no stamens downwards 3 pedicle breaks well above calyx 7 circular 8.0 RosBREED GDR FRFLAV_FRFLAVM_1-9FRFLAVscale 5 medium number of flowers UPOV FLDIA_FLDIAM_1-9FLDIAUPOVscale UPOV TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale not reflexed 1 no flowers 1 1 stamens shorter than receptacle Eikemo, et al. NCRI RPCCR_RPCCR1-8M_1-8RESISTscale RPO_RPOM_% USDA divides at 25% from crown 2 RPOAP_RPOAP%M_% USDA Knapp, et al. RMaPh_ RMaPhM_1-5RESISTscale UC Davis RVD_RVD1-9M_1-9RESISTScale University of California, Davis Pincot et al. 2020 NEM-RF_RPPM_Rf Pinkerton & Finn RPP_RPPM_Rf USDA divides 75% from crown 4 USDA FRDEV_FRDEVM_days UPOV SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale Feldmann, et al. UC Davis SVD_SVD1-5M_1-5SUSCEPscale white 1 globose-conic 5.0 UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale RosBREED BEARINGTYPE_BEARINGRBM_count GDR 1 highly resistant/no symptoms 1 does not remove EPILGT_EPILGTM_cm USDA FRYIELD_FRYIELDTWM_g USDA FRFRESHWT_FRFRESHWT5M_g RosBREED GDR very symptomatic 2.5 DNARATIO_DNADAPIICFM_DNA/stdDNAILF USDA Capocasa et al. 2008 Università Politecnica delle Marche FRFIRM_PENEM_g 1 no fruit set 2 very small to small 7 many FLDATE1_FLDATE1M_FLDATE1date USDA no fruit 1 Capocasa et al. 2008 Università Politecnica delle Marche FRAP_FRAPTROM_TroloxEQumoles/g FW susceptible S FLPETALNUM_FLPETALNUMM_average USDA 5FRW_FRFRESHWT5LM_g USDA GDR ECOGDDREQH_GDDHM_days RosBREED low yield 3 USDA TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq GDR CALYXSZ_CALYXSZM_1-3CALYXSZscale RosBREED Capocasa et al. 2008 FRAP_FRAPFSHM_FSHEQumoles/g fruit Università Politecnica delle Marche slightly symptomatic 1.5 USDA DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL RosBREED GDR CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale GDR RosBREED TREXPOS_TREXPOS0-1M_0-1TREXPOSscale outwards 2 USDA FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale 5 calyx attachment is sunken below shoulder 9 plant has stamens low density 3 3 level with surface 1 strongly below surface strongly below 1 USDA FLDATE2_FLDATE2M_date PLANTHT_PLANTHTM_cm USDA RosBREED GDR PPMF_PPMFM_0-1PMFscale RosBREED FLEVAL_FLEVALM_0-1FLEVALscale GDR GDR SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale RosBREED PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale RosBREED GDR BLDATE1_FLDATE1M_FLDATE1date USDA 5 medium RosBREED GDR TITA_TITAM_TITAMeq/100gCA pedicle does not break 1 USDA RPOAP_RPOAP1-9M_1-9RESISTscale USDA PLOIDY_FCDAPIICFM_x dark 1 6.0 fingerling RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale CPBR 1 upright very large 9.0 RosBREED GDR FRFIRM_FRFIRMM_1-9FRFIRMscale 1 calyx attachment raised above shoulder (necked) USDA FRCOL1_FRCOL1M_date RosBREED FRpH_pHM_pH GDR TITA_TITA0.5M_TITAMeq/100gCA USDA RosBREED GDR SKINGLOSS_SKINGLOSSM_1-9GLOSSscale 3 light orange UC Davis Jiménez, et al. RPCCR_RPCCR1-5M_1-5RESISTscale attribute TRUSSSIZ_TRUSSM_1-9scale UPOV FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale GDR RosBREED no fruit set 4 1 highly resistant/no symptoms ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV not remontant 1 9.0 wedged 7.0 light red stamens taller than receptacle 9 1 conic USDA FRSHAPE_FRSHAPEGGM_TXT inserted 1 1 upwards SSC_SSCM_SSCg/100g GDR RosBREED pedicle breaks just above calyx 5 raised 3 3 protruding 9 complete fruit set 1.0 very resistant, healthy small to medium 4 9 completely uniform/symmetrical and attractive RosBREED GDR TRUSSSIZ_TRUSSCOUNTM_ave LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW Biswas et al. 2019 University of Delaware RMYFR_RMYFRM_% USDA 7 cordiform USDA PLANTHABIT_PLHABM_1-9PLHABscale UPOV BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale GDR CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale RosBREED Pinkerton & Finn USDA RMH_RMHM_1-5RMHscale CPBR PLANTHABIT_PLHABM_1-3PLHABscale 1 divides close to crown RFOF_RFOF%M_% USDA Zurn, et al. USDA RUNNERDATE_FRUNNERM_date USDA STEMBREAK_PEDBRKM_text 3 few Y** stem breaks well above calyx Pincot, et al. UC Davis RFOF_RFOF1-5M_1-5RESISTscale conic-wedge 3 white 1 9.0 oblate sunken 1 PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale USDA long conic 1 RosBREED GDR SPOAP_SPOAPM_1-9SUSCEPscale 4 medium orange GDR RosBREED RPF_RPF0-5M_0-5RESISTscale 5 intermediate large to very large 8.0 very soft 1 high yield 1 ovate 4 9 dark red 7 many flowers divides right before fruit 5.0 very glossy 9 highly resistant/no symptoms 9 7 high acronym strongly uneven 5 USDA BOTRYTIS_RBCM_0-1BOTRYTISscale 3 calyx size larger than fruit diameter USDA CE_CROPEST_g RUNNERNUM_RUNNERNUMCT_count USDA RosBREED SPO_SPOM_1-9SUSCEPscale GDR FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale CPBR 2 yellow soft 1 RosBREED ACHENEVIABLE_ACHVIAM_% GDR 6.0 rhombic 1 even 1 spreading 9 extremely vigorous calyx size equal to fruit diameter 2 ECOGDDREQ_GDDFM_days RosBREED GDR 2 medium yield 2 slightly below surface very firm 9 Fernandez et al. RNEOP_RNEOP0-6M_0-6RESISTscale University of North Carolina high density 1 oblong 5.0 3 low 9.0 dark red very easily removed 9 9 blackish red TotalPhenolics_F-CM_mg/L_GallicAcidEQ GDR RosBREED 5.0 orange red USDA FLDATE100_FLDATE100M_FLDATE100date RVD_RVD%_% USDA 1 highly resistant/no symptoms V variable, no consistent breaking habit 2 slightly uneven 3 divides 50% from crown 9 tough semi-upright 2 PLOIDY_FCDAPIIBLM_x USDA USDA LFRW_FRFRESHWLGM_g reniform 1 medium 5 greenish 1 1 not uniform/malformed 3 early 1 highly susceptible/severe symptoms 4 slightly above USDA FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale hundreds 9 fully reflexed 9 2 partially remontant 9 overcropped highly susceptible/severe symptoms 5 pinkish white 1 4 above surface globose 3 globose-conic 5 9.0 very high entity 9 highly susceptible/severe symptoms 6.0 pink 2.0 mildly symptomatic even or very slightly uneven 1 6.0 medium to large medium red 8.0 same level 3 no runners 1 3 slightly uneven absent or very weak 1 erect 9 3 strongly uneven Y Stem breaks just above calyx, good for fresh market slightly below 2 5.0 medium Strawberry ontology 3 small 2 conic 1 dull 7.0 large Stamen presence Stamen The presence of stamens. presence STAMENPRES Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Cy-3-gluc method DNARATIO Plant Ratio of sample DNA to standard DNA. Plant DNA ratio DNA ratio Number of runners count method Harvest and count the number of runners collected from a single crown on a single day in a specific location. Titratable acidity NaOH method Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. 1-5 SUSCEP scale Susceptibility to Phomopsis obscurans method Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. 1-3 pubescence scale Fruit development Number of days from flowering to ripe fruit. Fruit development FRDEV DNA/std DNA_IBL 1-9 FRSHAPUNI GRIN scale Plant Crop estimate The amount of harvested fruit per plant per season. CE fruit yield flavor FRFLAV The flavor of fruit. Fruit flavor Fruit The plant resistance to necrosis caused by Neopestalotiopsis sp. Plant resistance to necrosis caused by Neopestalotiopsis sp. RNEOP resistance to necrosis caused by Neopestalotiopsis sp. Plant Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). Ferric reducing antioxidant power FSH method Observe the presence of flowers during each week in July, August and September. Everbearing flowering method 1-7 PEDBRK scale Estimate fruit yield and record it. Fruit yield 1-9 method Resistance to Meloidogyne hapla method Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. R-S category position Calyx CALYXPOS Calyx position The position of the calyx attachment relative to the fruit shoulder. Skin color color SKINCOL Skin Skin color on the sun side of a fully ripe fruit. Plant SPF Plant susceptibility to Phytophthora fragariae red stele root rot The plant susceptibility to red stele root rot caused by Phytophthora fragariae. susceptibility to Phytophthora fragariae red stele root rot Pedicle PEDICLEBREAK Pedicle breaking habit Pedicle breakage relative to the calyx. breaking habit Plant resistance to Verticillium dahliae Plant resistance to Verticillium dahliae wilt The plant resistance to verticillium wilt caused by Verticillium dahliae. RVD x Pedicle Pedicle breakage relative to the calyx. STEMBREAK breaking habit Stem breaking Skin The toughness of skin. toughness SKINTOUGH Skin toughness Skin color chromameter reflect L* brightness method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Leaf pubescence The density of trichomes on the leaf surface. Leaf LFPUBESC pubescence Fruit FRCOLORUNIF Fruit uniformity of color. uniformity of color Fruit uniformity of color 1-9 FRSHAPE RB scale count Weekly flowering evaluation Observe the plant weekly and record if it is flowering. Fruit Record the Julian date when first fruit ripens at a specific location. HARVDATE harvest date Fruit harvest date exposure The position of the truss relative to the foliage. Truss Truss exposure TREXPO Plant height method Measure the tallest trifoliate leaf from crown to base of leaflets. CALYXREFLX Calyx reflexion reflexion Reflexion of the calyx relative to the fruit shoulders. Calyx Plant RMaPh Plant resistance to Macrophomina phaseolina charcoal rot resistance to Macrophomina phaseolina charcoal rot The plant resistance to charcoal rot caused by Macrophomina phaseolina. Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight method flesh color Fruit Fruit flesh color FRFLCOL The color of fruit flesh. Flower Flower diameter The diameter of flower at fully open. diameter FLDIA Calyx size method Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. pH FRpH The pH of fruit. Fruit Fruit pH variable_of Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape UPOV method Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Fruit ripening trait The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA Fruit ripening trait FRRP 1-9 CALYXREM scale EVERBERIN Plant Everbearing bearing type Whether or not the plant has an everbearing habit Resistance to Phytophthora cactorum CR 1-5 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. set Fruit set FRUITSET Fruit The effeciency of fruit set at a specific location. FLDATE1 Flower date first Flower date first Julian date of first open flowers for an individual, whole plant, or population. Fruit drip loss Percent weight lost when frozen berries are thawed. drip loss Fruit DRIP LOSS Truss exposure 0-1 method Observe the truss location relative to the foliage canopy and record it. Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. SSC:TA method Growing degree days to first flowering. flower development ECOGDDREQ Plant flower development Plant Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. 1-5 RMH scale 1-9 FLDATE1REL UPOV scale 1-9 PLHAB scale Observe and rate the number of runners per plant. Number of runners 1-7 method Plant fruit development Plant fruit development Growing degree days to first harvest. ECOGDDREQH Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. 1-9 STAMENHGHT scale FRFIRM The firmness of fruit flesh. Fruit Fruit flesh firmness flesh firmness % Growing degree days flowering method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. The total number of daughter plantlets per plant harvested on a single day at a specific location. Runner daughters daughters Runner DAU TITA Fruit Fruit titratable acidity titratable acidity The titratable acidity content of fruit. The weight of fresh fruit. fresh weight largest Largest fruit weight LFRW Fruit Total anthocyanins Pg-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Plant height PLANTHT height Plant Height of tallest trifoliate leaf. 1-3 CALYXREFLX UPOV scale Leaf total antioxidant content total antioxidant content Leaf LFTOTANTIOX Total antioxidant content of leaves. 1-5 PEDUNCLEN scale Drip loss method Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Fruit length method Measure the length of the fruit from the calyx to the tip and record in mm. Achene color color The color of achene on the sun side of a fully ripe fruit. ACHENECOL Achene The plant susceptibility to verticillium wilt caused by Verticillium dahliae. susceptibility to Verticillium dahliae wilt Plant Plant susceptibility to Verticillium dahliae wilt SVD Fruit presence of powdery mildew PPMF The presence of powdery mildew on fruit. presence of powdery mildew Fruit Harvest all runners on a single day and measure the total length. Runner total length method Ferric reducing antioxidant power of fruit. FRAP ferric reducing antioxidant power Fruit Fruit ferric reducing antioxidant power Achene color 1-3 method Observe the average achene color on 3-5 fruit per genotype and record it. Achene Percentage of viable achenes ACHENEVIABLE viable The percentage of achenes that are viable. Plant resistance to Fusarium oxysporum f. sp. fragariae wilt The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Plant resistance to Fusarium oxysporum f. sp. fragariae wilt RFOF Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. 1-9 RESIST scale Plant resistance to Phomopsis obscurans leaf blight The plant resistance to leaf blight caused by Phomopsis obscurans. resistance to Phomopsis obscurans leaf blight RPO Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Plant susceptibility to Phomopsis obscurans leaf blight SPO The plant susceptibility to leaf blight caused by Phomopsis obscurans. susceptibility to Phomopsis obscurans leaf blight Plant Plant resistance to Pratylenchus penetrans root lesion nematode Plant RPP The plant resistance to root lesion nematode Pratylenchus penetrans. resistance to Pratylenchus penetrans root lesion nematode Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. Resistance to Colletotrichum acutatum anthracnose fruit rot % method flesh depth of color Fruit flesh depth of color Fruit Depth of fruit flesh color. FRFLCOLDEPTH Fruit FRSHAPE shape Fruit shape The shape of fruit. Truss exposure 1-5 method Observe the truss location relative to the foliage canopy and record it. Measure the diameter of the flowers at fully open Flower diameter method g 1-7 RUNNERNUM scale Observe and rate the number of runners per plant. Number of runners 1-9 method Fruit length FRLEN length Fruit Length of fruit from calyx to tip. Chroma Index Percentage of viable achenes method Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. Fruit yield 1-9 O method Estimate fruit yield and record it. Flower FLDATE2 date last Flower date last Julian date of last flowering for an individual, whole plant, or population. Epipodium measurement method Measure the length of the epipodium of the primary fruit from the calyx to the truss. Susceptibility to Diplocarpon earlianum method Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Fruit firmness method susceptibility to Mycosphaerella fragariae leaf spot SMYFR Plant susceptibility to Mycosphaerella fragariae leaf spot The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. Plant Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-3 method 1-9 CALYXREFLX scale Susceptibility to Colletotrichum gloeosporioides weekly method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Ferric reducing antioxidant power trolox method 1-9 FRYIELD Ordinal scale Skin color color SKINCOL Skin Skin color on the sun side of a fully ripe fruit. Plant RPF resistance to Phytophthora fragariae red stele root rot The plant resistance to red stele root rot caused by Phytophthora fragariae. Plant resistance to Phytophthora fragariae red stele root rot Resistance to Phytophthora fragariae 0-5 method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Mycosphaerella fragariae method Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. 1-3 ACHENECOL UPOV scale Plant bearing type Plant Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. BEARINGTYPE bearing type 1-5 RESIST scale average RTL Runner total length length total The total length of runners from a single plant harvested on a single day at a specific location. Runner Flower petal number Flower petal number FLPETALNUM Average flower petal number. Pedicel measurement method Measure the length of the pedicel of the primary fruit from the calyx to the truss. 1-3 RESIST scale Resistance to Verticillium dahliae 1-3 method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. date mg/L Pg-3-gluc kg−1 Reproductive factor cm Count the total number of daughter plants from a single plant harvested on a single day. Daughter plantlet count Meq NaOH/100g Plant susceptibility to leaf scorch caused by Diplocarpon earliana Plant SDE susceptibility to Diplocarpon earliana The plant susceptibility to leaf scorch caused by Diplocarpon earliana 1-10 SKINCOL UPOV scale Observe and rate average fruit shape on 5 fruit per genotype. Fruit shape GG method Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Titratable acidity citric acid method Flower date first relative method Observe the plant and record the relative flowering of the first open flowers. FLDATE1 Date mm Resistance to Fusarium oxysporum f. sp. fragariae % method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Presence or absence of central plug after calyx removal. CALYXPLUG plug Calyx plug Calyx Epipodium length Epipodium EPILGT Stem length of primary fruit from calyx to truss. length Resistance to Neopestalotiopsis sp. scale 0-6 Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants FLDATE1REL Flower Flower date first relative Relative date of first open flowers for an individual, whole plant, or population. date first relative Skin color 1-9 method Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Plant The plant resistance to Mycosphaerella fragariae. RMYFR Plant resistance to Mycosphaerella fragariae leaf spot resistance to Mycosphaerella fragariae leaf spot Susceptibility to Phytophthora fragariae method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-4 method 1-9 PLANTVIGOR scale Plant The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides SCG 1-9 FRGLOSS scale Weigh total fruit yield and record it. Fruit yield total weight method Resistance to Phytophthora cactorum CR % method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Flower date first method Observe the plant and record the date the first flowers open. Calyx reflexion method Observe the plant and record the reflexion of the calyx relative to the fruit shoulders mg/L Gallic Acid EQ resistance to Verticillium dahliae wilt Plant The plant resistance to verticillium wilt caused by Verticillium dahliae. Plant resistance to Verticillium dahliae wilt RVD Fruit Fruit shape uniformity. FRSHAPEUNIF shape uniformity Fruit shape uniformity Perpetual flowering RB method Record the number of weeks of flowering after week 11 from the first week of bloom. 1-5 FRCOLUNI scale 0-6 RESIST scale Flower diameter UPOV method Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. Depth of fruit flesh color Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. Resistance to Macrophomina phaseolina % method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Observe the plant and assess the erectness or spreading of the leaves along the ground. Plant habit scale method FLDATE100 Flower date 100% Julian date of peak flowering for an individual, whole plant, or population. Flower date 100% RUNNERDATE First runner date first emergence Runner The Julian date of appearance of the first runners. Stem length of primary fruit from calyx to truss. length Pedicle Pedicle length PEDICELLEN The plant resistance to leaf scorch caused by Diplocarpon earliana. Plant RDE Plant resistance to Diplocarpon earlianum leaf scorch resistance to Diplocarpon earlianum leaf scorch 1-9 ACHENECOL scale flesh firmness The firmness of fruit flesh. FRFIRM Fruit Fruit flesh firmness FRCOL1 Appearance of first color on fruit Fruit Appearance of first color on fruit. first color appearance Observe fruit appearance from not uniform to completely uniform. Fruit appearance method The weight of fresh fruit. Fruit fresh weight FRFRESHWT fresh weight Fruit SSC Fruit total water soluble solid content Fruit total water soluble solid content The soluble solids content of fruit. CALYXSZ Calyx Calyx size The size of the calyx. size Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae % method Record the number of days from first flowering to ripe fruit. Fruit development method Observe the number of fruit that a single plant sets in a specific location. Fruit set method Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. Soluble solids method Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Folin-Ciocalteu method Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Fruit flesh color method Plant ploidy Plant ploidy The number of sets of chromosomes in the nucleus. PLOIDY Fruit firmness penetrometer method Measure fruit firmness in grams using a hand-held penetrometer with a piston. Pedicle breakage method Observe where the pedicle breaks during harvest. Titratable acidity 0.5 g citric acid method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. 1-9 FLDIA UPOV scale Observe and rate the fruit skin color. Skin color 1-10 method 1-5 TREXPOS UPOV scale Fruit TPHE The total phenolic acids of fruit. total phenolic acids content Fruit total phenolic acids content DNA/std DNA_ILF Plant susceptibility to Podosphaera aphanis powdery mildew SPOAP The plant susceptibility to powdery mildew caused by Podosphaera aphanis. Plant susceptibility to Podosphaera aphanis Number of flowers per truss TRUSSSIZ Truss Truss size size method_of development Number of days from flowering to ripe fruit. FRD Fruit ripen days Fruit FSH EQ μmoles/g fruit 1-9 FRFIRM scale 0-1 CALPLUG scale 1-9 RUNNERNUM scale Presence of stamens 0-1 method Observe presence of anthers and record it. DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method mg/L Cy-3-gluc Eq Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight 1 method scale_of The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. Fruit resistance to Colletotrichum acutatum anthracnose fruit rot RAFR Fruit resistance to Colletotrichum acutatum anthracnose fruit rot 1-9 FRFLAV scale Fruit yield 1-4 method Estimate fruit yield and record it. development The weekly development of flowers. Flower Weekly flower evaluation FLEVAL Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Skin color chromameter reflect chroma index method FLDATE100 date habit PLANTHABIT The degree of erectness of the leaves. Plant habit Plant 0-1 STAMPRES scale Ease of calyx removal method Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Crop estimate method Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. grams Susceptibility to Podosphaera aphanis method Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. Fruit titratable acidity titratable acidity Fruit The titratable acidity content of fruit. TITA Petal number average method Count the number of petals on 10 flowers and average the results 5FRW Fruit fresh weight The weight of fresh fruit. Fruit weight Skin gloss Skin gloss The glossiness of skin. SKINGLOSS meters 1-3 ACHENEPOS scale HD Fruit harvest date Record the Julian date when first fruit ripens at a specific location. Harvest date Fruit pH 2 method Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Indication of resistance to Botrytis presence of grey mold Plant BOTRYTIS The presence of grey mold caused by Botrytis cinerea on the plant or fruit. Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Leaf total antioxidant assay method Achene color 1-9 method Observe the average achene color on 3-5 fruit per genotype and record it. The vigor of plant. Plant vigor vigor PLANTVIGOR Plant 1-9 SKINCOL scale Growing degree days harvest method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. 1-9 SUSCEP scale Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Susceptibility to Mycosphaerella fragariae method Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method RPCRR Plant resistance to Phytophthora cactorum root rot resistance to Phytophthora cactorum root rot Plant The plant resistance to root rot caused by Phytophthora cactorum. Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina method 1-5 CALYXPOS scale Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Susceptibility to Verticillium dahliae 1-5 method Achene The postion of achene relative to the fruit surface. position Achene position ACHENEPOS Number of flowers per truss estimation Observe the number of flowers per inflorescence. Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method Resistance to Diplocarpon earlianum method Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Color uniformity method Observe fruit color uniformity from even or very slighly unevento strongly uneven. GG scale Plant resistance to Meloidogyne hapla root knot nematode Plant resistance to Meloidogyne hapla root knot nematode RMH The plant resistance to root knot nematode Meloidogyne hapla. FLDATE2 date 1-4 ACHENEPOS UPOV scale 1-9 FRYIELD scale 1-3 CALYXPOS UPOV scale Meq/100g citric acid Observe the plant and record the date when at peak flowering. Flower date 100% method Julian date of first open flowers for an individual, whole plant, or population. Bloom date first BDF Flower date first Stembreak txt 0-1 BOTRYITIS scale days The plant resistance to crown rot caused by Phytophthora cactorum. RPCCR Plant resistance to Phytophthora cactorum crown rot resistance to Phytophthora cactorum crown rot Plant Trolox EQ μmoles/g FW resistance to Podosphaera aphanis powdery mildew RPOAP The plant resistance to powdery mildew caused by Podosphaera aphanis. Plant Plant resistance to Podosphaera aphanis powdery mildew Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae % method Runner RUNNERNUM Runner number The number of runners. number Fruit pH method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. 1-9 STAMPRES scale 0-5 SUSCEP scale Ease of calyx removal. Calyx CALYXREMOVE Calyx ease of removal ease of removal 1-3 PLHAB scale Fruit flavor method Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. 1-3 FRCOLUNI scale Stamen height The height of the stamens relative to the receptacles. STAMENHGHT height Stamen Observe average peduncle length and record it. Peduncle length method 1-9 FRFLCOL scale Observe the presence of powdery mildew on ripe fruit. Presence of powdery mildew on fruit method Plant vigor method Observe plant vigor of the two clones and record the average. The length of peduncle. PEDUNCLEN Peduncle Peduncle length length Resistance to Podosphaera aphanis 1-9 method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. 1-4 PERPFL UPOV scale Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. Fruit fresh weight 5L method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-3 method Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx plug method Fruit total anthocyanin content total anthocyanin content Fruit ATCYN The total anthocyanins of fruit. TACY total anthocyanin content Fruit The total anthocyanins of fruit. Total anthocyanins Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Flower date last method Observe the plant and record the date when at the end of flowering. Fruit shape RB method Observe and rate average fruit shape on 3-5 fruit per genotype. 0-1 FLEVAL scale First runner date method Observe the plant and record the Julian date the first runners emerge at a specific location. 1-8 RESIST scale 0-1 EVER scale 1-4 FRYIELD scale 1-3 CALYXSZ scale 1-9 SKINTOUGH scale Stamen height method Observe and rate the height of the stamens relative to the receptacle at flower opening. 1-9 FRSHAPE UPOV scale 0-7 TRUSS scale Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Perpetual flowering method Fruit fresh weight largest method Harvest and record the largest fruit over the harvest season. Ratio Plant FRYIELD The fruit yield of one individual. fruit yield Plant fruit yield 0-1 PMF scale Resistance to Podosphaera aphanis % method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Presence of stamens 1-9 method Observe presence of anthers and record it. Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method FRpH 0-1 TREXPOS scale Calyx position 1-5 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Resistance to Verticillium dahliae scale 1-9 Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface DNA ration determination using DAPI with internal standard = iceberg lettuce method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. 1-9 FRUITSET scale Record the Julian date when first fruit ripens at a specific location. Harvest date method Luminance g/100g