TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale UPOV globose 3 highly susceptible/severe symptoms 8 UC Davis RPCCR_RPCCR1-5M_1-5RESISTscale Jiménez, et al. 7.0 oblate UPOV CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale RPCCR_RPCCR%M_% USDA 9 highly susceptible/severe symptoms 1 stamens shorter than receptacle 1 very early STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale RosBREED GDR pedicle breaks just above calyx 5 FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale RosBREED GDR USDA FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale SKINCOL_SKINCOL1-9M_1-9SKINCOLscale RosBREED GDR RosBREED GDR FRFIRM_FRFIRMM_1-9FRFIRMscale RosBREED GDR FLEVAL_FLEVALM_0-1FLEVALscale globose-conic 5 SKINCOL_REFLECTCIXM_ChromaIndex Capocasa et al. 2008 Università Politecnica delle Marche 3 light orange FRRAFR_FRAFR%M_% University of Florida Salinas et al. 2019 rhombic 6.0 PLANTHABIT_PLHABM_1-9PLHABscale USDA USDA STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale USDA FLPETALNUM_FLPETALNUMM_average globose 4 1 upright few 3 SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale UPOV TRUSSSIZ_TRUSSM_1-9scale UPOV 6.0 medium to large USDA PLOIDY_FCDAPIIBLM_x level with fruit 2 USDA RDE_REDM_1-9RESISTscale RosBREED GDR CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale UPOV ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale GDR RosBREED FRSHAPE_FRSHAPEGGM_TXT USDA RPOAP_RPOAP1-9M_1-9RESISTscale USDA FRDEV_FRDEVM_days USDA 3 conic-wedge RVD_RVD%_% USDA UC Davis Pincot, et al. RFOF_RFOF1-5M_1-5RESISTscale TREXPOS_TREXPOS0-1M_0-1TREXPOSscale RosBREED GDR University of North Carolina RNEOP_RNEOP0-6M_0-6RESISTscale Fernandez et al. USDA FRpH_pH2M_pH 3 cordate RosBREED GDR FRFLCOL_FRFLCOLM_1-9FRFLCOLscale 1 no fruit 1 soft pedicle breaks well above calyx 7 9.0 very high divides 75% from crown 4 USDA FLDATE2_FLDATE2M_date Capocasa et al. 2008 FRAP_FRAPTROM_TroloxEQumoles/g FW Università Politecnica delle Marche 3 raised 9 tough FLDATE100_FLDATE100M_FLDATE100date USDA USDA FLDATE1_FLDATE1M_FLDATE1date plant has stamens 9 2 outwards PLOIDY_FCDAPIICFM_x USDA no runners 1 RosBREED GDR ACHENEVIABLE_ACHVIAM_% EVERBERIN_EVER0-1M_0-1EVERscale USDA upwards 1 SKINGLOSS_SKINGLOSSM_1-9GLOSSscale RosBREED GDR 3.0 dead RPP_RPPM_Rf USDA NEM-RF_RPPM_Rf Pinkerton & Finn USDA BOTRYTIS_RBCM_0-1BOTRYTISscale USDA FRYIELD_FRYIELDTWM_g LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW University of Delaware Biswas et al. 2019 5.0 oblong RosBREED GDR CALYXSZ_CALYXSZM_1-3CALYXSZscale RPO_RPOM_% USDA 3 same level RosBREED GDR RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale Capocasa et al. 2008 TITA_TITAM_TITAMeqNAOH/100g Università Politecnica delle Marche 1 even or very slightly uneven 2 even SMYFR_SMYFRM_1-9SUSCEPscale RosBREED GDR SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale RosBREED GDR RosBREED GDR CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale day neutral 4 no fruit set 1 1 spreading STEMBREAK_PEDBRKM_text USDA RTL_RUNTOTLENM_m USDA 3 red 9 very glossy stem does not break, berry separates from calyx easily N blackish red 9 DAU_RUNDAUM_count USDA greenish 1 USDA Zurn, et al. RMaPh_ RMaPh%M_ % USDA FRD_FRRIPEM_days 3 small SCG_SCGWKM_R-Scategory University of Florida Chandra et al. 2021 9 very light brown or green BLDATE1_FLDATE1M_FLDATE1date USDA CPBR PLANTHABIT_PLHABM_1-3PLHABscale 3 downwards strongly above 5.0 3 breakage is inconsistent RPOAP_RPOAP%M_% USDA RosBREED GDR SDE_SDEM_1-9SUSCEPscale white 1 GDR TotalPhenolics_F-CM_mg/L_GallicAcidEQ RosBREED RosBREED GDR FRFRESHWT_FRFRESHWT5M_g FLDIA_FLDIAM_1-9FLDIAUPOVscale UPOV RosBREED GDR FRFLAV_FRFLAVM_1-9FRFLAVscale RosBREED GDR FRYIELD_FRYIELD1-9M_1-9FRYIELDscale RVD_RVD1-9M_1-9RESISTScale Pincot et al. 2020 University of California, Davis USDA FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale mildly symptomatic 2.0 USDA STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale medium yield 2 3 strongly uneven RPF_RPF%M_% USDA CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale RosBREED GDR dark 1 UPOV FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale DRIPLOSS_DRIPLOSSM_g RosBREED GDR very small to small 2 USDA FRLEN_FRLENM_mm 7 late many 7 7.0 large CE_CROPEST_g USDA variable, no consistent breaking habit V spreading 3 3 calyx attachment is even with fruit 3 low density conic 1 completely uniform/symmetrical and attractive 9 RMYFR_RMYFRM_% USDA RosBREED GDR HARVDATE_HARVDATEM_date 1 highly susceptible/severe symptoms 9 complete fruit set acronym TITA_TITA0.5M_TITAMeq/100gCA USDA USDA FRYIELD_FRYIELD1-4M_1-4FRYIELDscale 1 plant has no stamens orange red 5.0 medium red 8.0 RosBREED GDR BEARINGTYPE_BEARINGRBM_count 2 slightly below RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale CPBR LFRW_FRFRESHWLGM_g USDA RosBREED GDR ECOGDDREQ_GDDFM_days 3 early medium 5 Feldmann, et al. SVD_SVD1-5M_1-5SUSCEPscale UC Davis 7.0 light red HD_FIRSTHARVM_date USDA circular 8.0 5FRW_FRFRESHWT5LM_g USDA stamens taller than receptacle 9 SPO_SPOM_1-9SUSCEPscale RosBREED GDR semi-upright 2 very firm 9 EPILGT_EPILGTM_cm USDA 1 inserted UPOV BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale calyx size larger than fruit diameter 3 USDA RFOF_RFOF%M_% Zurn, et al. Oregon State RVD_RVD1-3M_1-3RESISTscale Vining, et al. Università Politecnica delle Marche Capocasa et al. 2008 FRFIRM_PENEM_g RPF_RPF0-5M_0-5RESISTscale RosBREED GDR 1 dull strongly uneven 5 RosBREED TRUSSSIZ_TRUSSCOUNTM_ave GDR RUNNERNUM_RUNNERNUMCT_count USDA RosBREED GDR ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq attribute R resistant USDA DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL 1 high yield FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale UPOV ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale RosBREED GDR University of Delaware Biswas et al. 2019 LFPUBESC_LFPUBESCM_1-3LFPUBESCscale PPMF_PPMFM_0-1PMFscale RosBREED GDR USDA ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq FLDIA_FLDIAM_cm UPOV PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale RosBREED GDR 1 long conic 1 dead dark red 9 3 low RosBREED GDR ECOGDDREQH_GDDHM_days 2 slightly uneven 5 intermediate fully reflexed 9 FRFLCOLDEPTH_FRCOLDEPTHM_% RosBREED GDR 4 above surface long conic 2 FRAP_FRAPFSHM_FSHEQumoles/g fruit Università Politecnica delle Marche Capocasa et al. 2008 CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale USDA 6.0 pink highly susceptible/severe symptoms 1 many flowers 7 RosBREED GDR PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale highly resistant/no symptoms 9 TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA yellow 2 USDA PEDICELLEN_PEDICELLENM_cm FRUITSET_FRSETM_1-9FRUITSETscale USDA slightly uneven 3 TITA_TITAM_TITAMeq/100gCA RosBREED GDR 4 slightly above 9 highly resistant/no symptoms strongly below surface 1 Knapp, et al. RMaPh_ RMaPhM_1-5RESISTscale UC Davis sunken 1 very sweet, no off-flavors 9 medium density 2 divides at 25% from crown 2 2 conic 2 slightly below surface medium 5.0 9.0 very large Pinkerton & Finn RMH_RMHM_1-5RMHscale USDA 9.0 very late highly resistant/no symptoms 1 Capocasa et al. 2008 SKINCOL_REFLECTLM_L* Università Politecnica delle Marche yellowish white 2 3 low yield UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale CPBR FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale 1 no fruit no flowers 1 USDA RUNNERDATE_FRUNNERM_date large to very large 8.0 USDA PLANTHT_PLANTHTM_cm 1 highly resistant/no symptoms blackish red 10.0 GDR SPOAP_SPOAPM_1-9SUSCEPscale RosBREED globose-conic 5.0 medium orange 4 USDA RPCRR_RPC%M_% entity 5 highly susceptible/severe symptoms 1 calyx attachment raised above shoulder (necked) Eikemo, et al. RPCCR_RPCCR1-8M_1-8RESISTscale NCRI erect 9 4 small to medium 6.0 fingerling susceptible S high density 1 Stem breaks just above calyx, good for fresh market Y FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale UPOV 1 pinkish white USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg 1.0 very resistant, healthy 2 calyx size equal to fruit diameter Strawberry ontology RosBREED GDR SSC_SSCM_SSCg/100g ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV USDA PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale 1 divides close to crown 1 reniform protruding 3 USDA FRCOL1_FRCOL1M_date stem breaks well above calyx Y** even 1 RosBREED GDR SPF_SPFM_0-5SUSCEPscale 1 does not remove level with surface 3 5 calyx attachment is sunken below shoulder USDA DNARATIO_DNADAPIICFM_DNA/stdDNAILF FRRP_FRRPM_Ratio USDA pedicle does not break 1 divides 50% from crown 3 1 not uniform/malformed 2 partially remontant 5 medium number of flowers calyx size smaller than fruit diameter 1 9 overcropped RosBREED GDR FRpH_pHM_pH 3 fully remontant extremely vigorous 9 1 highly resistant/no symptoms not remontant 1 5 medium 2.5 very symptomatic dark red 9.0 1 strongly below 1 not sweet, bad off-flavors 1 not reflexed white 1 3 few flowers 4 ovate wedged 9.0 4 no fruit set very easily removed 9 very soft 1 9 hundreds absent or very weak 1 high 7 very small 1 5.0 divides right before fruit 7 cordiform oblate 9.0 1.5 slightly symptomatic 1-3 CALYXSZ scale % Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Flower diameter UPOV method count DNA/std DNA_IBL Ferric reducing antioxidant power of fruit. ferric reducing antioxidant power Fruit Fruit ferric reducing antioxidant power FRAP 1-9 FRGLOSS scale Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. Calyx size method total anthocyanin content Fruit TACY Total anthocyanins The total anthocyanins of fruit. Bloom date first date first Flower BDF Julian date of first open flowers for an individual, whole plant, or population. Count the total number of daughter plants from a single plant harvested on a single day. Daughter plantlet count 1-9 FLDATE1REL UPOV scale SKINTOUGH Skin toughness The toughness of skin. Skin toughness Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Record the number of weeks of flowering after week 11 from the first week of bloom. Perpetual flowering RB method susceptibility to Podosphaera aphanis Plant susceptibility to Podosphaera aphanis powdery mildew SPOAP Plant The plant susceptibility to powdery mildew caused by Podosphaera aphanis. habit Plant habit The degree of erectness of the leaves. PLANTHABIT Plant 0-7 TRUSS scale 1-9 SKINCOL scale Titratable acidity NaOH method Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. CALYXREMOVE ease of removal Calyx ease of removal Calyx Ease of calyx removal. Plant vigor method Observe plant vigor of the two clones and record the average. Weekly flower evaluation FLEVAL Flower development The weekly development of flowers. Runner number The number of runners. Runner number RUNNERNUM Ferric reducing antioxidant power trolox method Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Pedicle breakage relative to the calyx. breaking habit Pedicle breaking habit PEDICLEBREAK Pedicle date first Flower FLDATE1 Julian date of first open flowers for an individual, whole plant, or population. Flower date first Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape UPOV method Fruit pH method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. Fruit Number of days from flowering to ripe fruit. development FRD Fruit ripen days Reproductive factor viable Achene The percentage of achenes that are viable. Percentage of viable achenes ACHENEVIABLE flesh firmness The firmness of fruit flesh. Fruit FRFIRM Fruit flesh firmness Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. SSC:TA method susceptibility to Phomopsis obscurans leaf blight SPO Plant susceptibility to Phomopsis obscurans leaf blight Plant The plant susceptibility to leaf blight caused by Phomopsis obscurans. cm Fruit length method Measure the length of the fruit from the calyx to the tip and record in mm. titratable acidity Fruit TITA The titratable acidity content of fruit. Fruit titratable acidity 1-3 CALYXREFLX UPOV scale Fruit The titratable acidity content of fruit. Fruit titratable acidity titratable acidity TITA Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. Soluble solids method The plant resistance to powdery mildew caused by Podosphaera aphanis. Plant resistance to Podosphaera aphanis powdery mildew Plant resistance to Podosphaera aphanis powdery mildew RPOAP Fruit pH 2 method Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. g Truss exposure 1-5 method Observe the truss location relative to the foliage canopy and record it. Height of tallest trifoliate leaf. PLANTHT height Plant height Plant Record the Julian date when first fruit ripens at a specific location. harvest date HARVDATE Fruit Fruit harvest date grams 1-3 CALYXPOS UPOV scale Number of flowers per truss TRUSSSIZ size Truss Truss size 1-9 PLANTVIGOR scale Susceptibility to Colletotrichum gloeosporioides weekly method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Resistance to Diplocarpon earlianum method Measure the tallest trifoliate leaf from crown to base of leaflets. Plant height method 1-9 PLHAB scale Meq/100g citric acid Observe average peduncle length and record it. Peduncle length method 1-5 RMH scale Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-3 method Plant susceptibility to Phytophthora fragariae red stele root rot Plant SPF The plant susceptibility to red stele root rot caused by Phytophthora fragariae. susceptibility to Phytophthora fragariae red stele root rot Resistance to Verticillium dahliae % method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. FLDATE2 date Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Perpetual flowering method ACHENEPOS position Achene position The postion of achene relative to the fruit surface. Achene Number of runners 1-9 method Observe and rate the number of runners per plant. Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Susceptibility to Mycosphaerella fragariae method Harvest and record the largest fruit over the harvest season. Fruit fresh weight largest method 1-5 RESIST scale position Calyx CALYXPOS Calyx position The position of the calyx attachment relative to the fruit shoulder. Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae % method The effeciency of fruit set at a specific location. Fruit Fruit set set FRUITSET 1-10 SKINCOL UPOV scale PLANTVIGOR Plant vigor Plant vigor The vigor of plant. Leaf total antioxidant assay method Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina method 1-9 FRFLAV scale LFTOTANTIOX total antioxidant content Total antioxidant content of leaves. Leaf total antioxidant content Leaf 5FRW fresh weight Fruit weight Fruit The weight of fresh fruit. Fruit firmness method Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Skin color on the sun side of a fully ripe fruit. color SKINCOL Skin Skin color mg/L Pg-3-gluc kg−1 Count the number of petals on 10 flowers and average the results Petal number average method Reflexion of the calyx relative to the fruit shoulders. Calyx reflexion reflexion CALYXREFLX Calyx Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method mm resistance to Pratylenchus penetrans root lesion nematode Plant The plant resistance to root lesion nematode Pratylenchus penetrans. Plant resistance to Pratylenchus penetrans root lesion nematode RPP The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA FRRP ripening trait Fruit ripening trait Fruit CALYXPLUG plug Calyx Presence or absence of central plug after calyx removal. Calyx plug Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-3 method FRSHAPE Fruit Fruit shape The shape of fruit. shape Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina % method Calyx plug method Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Plant Plant DNA ratio DNA ratio DNARATIO Ratio of sample DNA to standard DNA. Plant SDE The plant susceptibility to leaf scorch caused by Diplocarpon earliana susceptibility to Diplocarpon earliana Plant susceptibility to leaf scorch caused by Diplocarpon earliana Observe where the pedicle breaks during harvest. Pedicle breakage method Fruit flavor method Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. resistance to Meloidogyne hapla root knot nematode Plant RMH The plant resistance to root knot nematode Meloidogyne hapla. Plant resistance to Meloidogyne hapla root knot nematode Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. 1-9 FRFLCOL scale Observe the plant and record the date the first flowers open. Flower date first method The plant resistance to charcoal rot caused by Macrophomina phaseolina. Plant resistance to Macrophomina phaseolina charcoal rot Plant resistance to Macrophomina phaseolina charcoal rot RMaPh Stamen height Stamen STAMENHGHT The height of the stamens relative to the receptacles. height Achene position 1-4 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Epipodium EPILGT Epipodium length length Stem length of primary fruit from calyx to truss. Fruit development method Record the number of days from first flowering to ripe fruit. Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. SCG susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant 1-9 FRYIELD scale 1-9 RUNNERNUM scale 0-1 PMF scale CE fruit yield Crop estimate The amount of harvested fruit per plant per season. Plant 1-9 FRFIRM scale Observe and rate average fruit shape on 5 fruit per genotype. Fruit shape GG method Fruit flavor FRFLAV flavor The flavor of fruit. Fruit Observe fruit color uniformity from even or very slighly unevento strongly uneven. Color uniformity method date Fruit flesh firmness Fruit flesh firmness The firmness of fruit flesh. FRFIRM 1-9 FRSHAPE RB scale Susceptibility to Phytophthora fragariae method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. Fruit Appearance of first color on fruit FRCOL1 Appearance of first color on fruit. first color appearance 1-4 FRYIELD scale shape uniformity Fruit FRSHAPEUNIF Fruit shape uniformity Fruit shape uniformity. EVERBERIN Whether or not the plant has an everbearing habit Plant Everbearing bearing type The soluble solids content of fruit. total water soluble solid content Fruit Fruit total water soluble solid content SSC 1-9 STAMENHGHT scale RTL Runner The total length of runners from a single plant harvested on a single day at a specific location. length total Runner total length 0-1 CALPLUG scale Skin toughness method Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. FLDATE100 date Achene ACHENECOL The color of achene on the sun side of a fully ripe fruit. Achene color color SMYFR Plant susceptibility to Mycosphaerella fragariae leaf spot The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. Plant susceptibility to Mycosphaerella fragariae leaf spot Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Resistance to Mycosphaerella fragariae method flesh color The color of fruit flesh. FRFLCOL Fruit flesh color Fruit Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. Fruit yield 1-4 method Estimate fruit yield and record it. Fruit yield 1-9 O method Estimate fruit yield and record it. x FRpH Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Skin color chromameter reflect chroma index method RVD Plant The plant resistance to verticillium wilt caused by Verticillium dahliae. Plant resistance to Verticillium dahliae wilt resistance to Verticillium dahliae Observe the plant and record the relative flowering of the first open flowers. Flower date first relative method 0-1 EVER scale Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Crop estimate method First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface The plant resistance to crown rot caused by Phytophthora cactorum. Plant RPCCR Plant resistance to Phytophthora cactorum crown rot resistance to Phytophthora cactorum crown rot 0-5 SUSCEP scale Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape RB method 0-1 STAMPRES scale Fruit pH FRpH pH Fruit The pH of fruit. mg/L Cy-3-gluc Eq Observe the presence of flowers during each week in July, August and September. Everbearing flowering method Plant The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. RFOF resistance to Fusarium oxysporum f. sp. fragariae wilt Plant resistance to Fusarium oxysporum f. sp. fragariae wilt Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. Susceptibility to Podosphaera aphanis method Pedicel measurement method Measure the length of the pedicel of the primary fruit from the calyx to the truss. The presence of stamens. presence Stamen presence Stamen STAMENPRES 1-4 PERPFL UPOV scale DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method resistance to Phytophthora fragariae red stele root rot Plant RPF Plant resistance to Phytophthora fragariae red stele root rot The plant resistance to red stele root rot caused by Phytophthora fragariae. date last Julian date of last flowering for an individual, whole plant, or population. FLDATE2 Flower date last Flower Observe and rate average fruit skin gloss on 3-5 fruit per genotype. Skin gloss method 1-3 RESIST scale Ferric reducing antioxidant power FSH method resistance to necrosis caused by Neopestalotiopsis sp. Plant resistance to necrosis caused by Neopestalotiopsis sp. RNEOP Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). Plant The plant resistance to necrosis caused by Neopestalotiopsis sp. PEDUNCLEN The length of peduncle. length Peduncle length Peduncle 1-9 ACHENECOL scale average 1-9 SUSCEP scale Achene color 1-9 method Observe the average achene color on 3-5 fruit per genotype and record it. Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants Resistance to Neopestalotiopsis sp. scale 0-6 Observe the plant and record the date when at the end of flowering. Flower date last method Stem breaking breaking habit STEMBREAK Pedicle Pedicle breakage relative to the calyx. 1-3 ACHENECOL UPOV scale meters Fruit firmness penetrometer method Measure fruit firmness in grams using a hand-held penetrometer with a piston. CALYXSZ The size of the calyx. size Calyx size Calyx Runner First runner date The Julian date of appearance of the first runners. first emergence RUNNERDATE Growing degree days harvest method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. 1-9 FRYIELD Ordinal scale Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Resistance to Meloidogyne hapla method Plant Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. BEARINGTYPE bearing type Plant bearing type RPCRR resistance to Phytophthora cactorum root rot Plant Plant resistance to Phytophthora cactorum root rot The plant resistance to root rot caused by Phytophthora cactorum. 1-9 SKINTOUGH scale Fruit fresh weight 5L method Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. 1-9 RESIST scale Meq NaOH/100g days Plant fruit development ECOGDDREQH Plant fruit development Growing degree days to first harvest. FRCOLORUNIF Fruit uniformity of color. uniformity of color Fruit Fruit uniformity of color Fruit yield 1-9 method Estimate fruit yield and record it. Observe presence of anthers and record it. Presence of stamens 1-9 method FSH EQ μmoles/g fruit Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-3 method fruit yield The fruit yield of one individual. Plant fruit yield FRYIELD Plant Observe the plant and record the reflexion of the calyx relative to the fruit shoulders Calyx reflexion method scale_of DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. DNA ration determination using DAPI with internal standard = iceberg lettuce method Titratable acidity 0.5 g citric acid method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. FLDATE1REL date first relative Flower date first relative Flower Relative date of first open flowers for an individual, whole plant, or population. Fruit Percent weight lost when frozen berries are thawed. DRIP LOSS Fruit drip loss drip loss R-S category Depth of fruit flesh color Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. 0-1 BOTRYITIS scale FRFRESHWT fresh weight Fruit Fruit fresh weight The weight of fresh fruit. Fruit length Length of fruit from calyx to tip. Fruit length FRLEN Weigh total fruit yield and record it. Fruit yield total weight method Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. The plant resistance to Mycosphaerella fragariae. Plant resistance to Mycosphaerella fragariae leaf spot resistance to Mycosphaerella fragariae leaf spot Plant RMYFR The density of trichomes on the leaf surface. Leaf pubescence Leaf LFPUBESC pubescence Flower diameter The diameter of flower at fully open. diameter Flower FLDIA Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR 1-5 method 1-3 PLHAB scale Resistance to Podosphaera aphanis % method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. method_of Folin-Ciocalteu method Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Resistance to Phytophthora cactorum CR % method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. DNA/std DNA_ILF Observe presence of anthers and record it. Presence of stamens 0-1 method Plant The number of sets of chromosomes in the nucleus. Plant ploidy ploidy PLOIDY exposure TREXPO Truss Truss exposure The position of the truss relative to the foliage. Drip loss method Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Observe the presence of powdery mildew on ripe fruit. Presence of powdery mildew on fruit method 1-9 CALYXREFLX scale Fruit flesh color method Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Number of flowers per truss estimation Observe the number of flowers per inflorescence. The glossiness of skin. gloss SKINGLOSS Skin gloss Skin Skin color 1-9 method Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. 1-3 pubescence scale Trolox EQ μmoles/g FW Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Resistance to Verticillium dahliae scale 1-9 Skin color Skin color on the sun side of a fully ripe fruit. Skin color SKINCOL presence of grey mold BOTRYTIS Plant The presence of grey mold caused by Botrytis cinerea on the plant or fruit. Indication of resistance to Botrytis 0-1 FLEVAL scale Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. Susceptibility to Verticillium dahliae 1-5 method 1-5 TREXPOS UPOV scale FLDATE1 Date Runner daughters DAU daughters Runner The total number of daughter plantlets per plant harvested on a single day at a specific location. 1-9 STAMPRES scale SVD The plant susceptibility to verticillium wilt caused by Verticillium dahliae. susceptibility to Verticillium dahliae wilt Plant susceptibility to Verticillium dahliae wilt Plant Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Skin color chromameter reflect L* brightness method Flower date 100% method Observe the plant and record the date when at peak flowering. 1-5 SUSCEP scale Truss exposure 0-1 method Observe the truss location relative to the foliage canopy and record it. Harvest date Fruit harvest date HD Record the Julian date when first fruit ripens at a specific location. Number of runners 1-7 method Observe and rate the number of runners per plant. 1-9 FRSHAPE UPOV scale Measure the diameter of the flowers at fully open Flower diameter method Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. 1-9 FRUITSET scale 1-5 PEDUNCLEN scale The presence of powdery mildew on fruit. Fruit presence of powdery mildew presence of powdery mildew Fruit PPMF Fruit fresh weight method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. 1-5 CALYXPOS scale Total anthocyanins Pg-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Luminance resistance to Colletotrichum acutatum anthracnose fruit rot RAFR The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. Fruit Fruit resistance to Colletotrichum acutatum anthracnose fruit rot Ease of calyx removal method Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Plant resistance to Diplocarpon earlianum leaf scorch The plant resistance to leaf scorch caused by Diplocarpon earliana. RDE Plant resistance to Diplocarpon earlianum leaf scorch Flower petal number Flower petal number FLPETALNUM Average flower petal number. Skin color 1-10 method Observe and rate the fruit skin color. Plant resistance to Phomopsis obscurans leaf blight RPO resistance to Phomopsis obscurans leaf blight The plant resistance to leaf blight caused by Phomopsis obscurans. Harvest and count the number of runners collected from a single crown on a single day in a specific location. Number of runners count method Growing degree days to first flowering. ECOGDDREQ Plant Plant flower development flower development 1-5 FRCOLUNI scale Ratio mg/L Gallic Acid EQ Susceptibility to Phomopsis obscurans method Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Stamen height method Observe and rate the height of the stamens relative to the receptacle at flower opening. fresh weight largest The weight of fresh fruit. Largest fruit weight LFRW Fruit Resistance to Verticillium dahliae 1-3 method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. 1-3 FRCOLUNI scale Plant habit scale method Observe the plant and assess the erectness or spreading of the leaves along the ground. Observe the plant weekly and record if it is flowering. Weekly flowering evaluation Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit fresh weight 1 method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days flowering method Depth of fruit flesh color. flesh depth of color Fruit Fruit flesh depth of color FRFLCOLDEPTH Stembreak txt Runner total length method Harvest all runners on a single day and measure the total length. 1-9 FRSHAPUNI GRIN scale Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Cy-3-gluc method date 100% Flower FLDATE100 Flower date 100% Julian date of peak flowering for an individual, whole plant, or population. Fruit total phenolic acids content TPHE total phenolic acids content The total phenolic acids of fruit. Fruit Resistance to Podosphaera aphanis 1-9 method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Calyx position 1-5 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. 1-7 PEDBRK scale 1-3 ACHENEPOS scale 1-9 FLDIA UPOV scale Titratable acidity citric acid method Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Fruit appearance method Observe fruit appearance from not uniform to completely uniform. 0-1 TREXPOS scale Resistance to Fusarium oxysporum f. sp. fragariae % method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. 1-7 RUNNERNUM scale Chroma Index Epipodium measurement method Measure the length of the epipodium of the primary fruit from the calyx to the truss. g/100g Susceptibility to Diplocarpon earlianum method Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Resistance to Phytophthora fragariae 0-5 method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. 1-8 RESIST scale 0-6 RESIST scale 1-4 ACHENEPOS UPOV scale Observe the number of fruit that a single plant sets in a specific location. Fruit set method 1-9 CALYXREM scale First runner date method Observe the plant and record the Julian date the first runners emerge at a specific location. development FRDEV Fruit development Fruit Number of days from flowering to ripe fruit. GG scale Plant resistance to Verticillium dahliae wilt RVD Plant The plant resistance to verticillium wilt caused by Verticillium dahliae. resistance to Verticillium dahliae wilt Stem length of primary fruit from calyx to truss. Pedicle Pedicle length PEDICELLEN length Harvest date method Record the Julian date when first fruit ripens at a specific location. Fruit total anthocyanin content Fruit The total anthocyanins of fruit. ATCYN total anthocyanin content Resistance to Colletotrichum acutatum anthracnose fruit rot % method Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. Percentage of viable achenes method Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method variable_of