Strawberry ontology FLEVAL_FLEVALM_0-1FLEVALscale RosBREED GDR 1 highly resistant/no symptoms small to medium 4 RosBREED GDR SPOAP_SPOAPM_1-9SUSCEPscale PEDICELLEN_PEDICELLENM_cm USDA Biswas et al. 2019 LFPUBESC_LFPUBESCM_1-3LFPUBESCscale University of Delaware oblong 5.0 no runners 1 GDR RPF_RPF0-5M_0-5RESISTscale RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale RosBREED GDR CE_CROPEST_g USDA 2 semi-upright USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg 1 highly resistant/no symptoms cordate 3 RosBREED GDR ACHENEVIABLE_ACHVIAM_% RosBREED GDR FRFLAV_FRFLAVM_1-9FRFLAVscale 3 few flowers RNEOP_RNEOP0-6M_0-6RESISTscale Fernandez et al. University of North Carolina USDA RUNNERDATE_FRUNNERM_date FLDIA_FLDIAM_cm UPOV 8.0 medium red SVD_SVD1-5M_1-5SUSCEPscale UC Davis Feldmann, et al. RPCCR_RPCCR%M_% USDA SKINGLOSS_SKINGLOSSM_1-9GLOSSscale RosBREED GDR globose-conic 5 8.0 circular FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale USDA USDA RPCRR_RPC%M_% FRLEN_FRLENM_mm USDA large to very large 8.0 USDA RVD_RVD%_% many 7 DRIPLOSS_DRIPLOSSM_g RosBREED GDR CPBR PLANTHABIT_PLHABM_1-3PLHABscale CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale UPOV 7.0 oblate USDA FRD_FRRIPEM_days 9 highly resistant/no symptoms RosBREED GDR PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale FLDATE2_FLDATE2M_date USDA 5 calyx attachment is sunken below shoulder ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale RosBREED GDR pink 6.0 TRUSSSIZ_TRUSSCOUNTM_ave RosBREED GDR RosBREED GDR TITA_TITAM_TITAMeq/100gCA USDA RPO_RPOM_% S susceptible late 7 3 breakage is inconsistent 9 overcropped RosBREED GDR HARVDATE_HARVDATEM_date highly susceptible/severe symptoms 5 GDR RosBREED PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale RosBREED FRFLCOL_FRFLCOLM_1-9FRFLCOLscale GDR SPF_SPFM_0-5SUSCEPscale RosBREED GDR ECOGDDREQ_GDDFM_days RosBREED GDR not sweet, bad off-flavors 1 UC Davis Knapp, et al. RMaPh_ RMaPhM_1-5RESISTscale UPOV BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale USDA BLDATE1_FLDATE1M_FLDATE1date 9.0 oblate ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV 1 high yield 1 not uniform/malformed TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale UPOV 5.0 orange red USDA LFRW_FRFRESHWLGM_g 9 erect DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL USDA white 1 even or very slightly uneven 1 1 long conic USDA FLDATE100_FLDATE100M_FLDATE100date 5 medium medium to large 6.0 RosBREED GDR SPO_SPOM_1-9SUSCEPscale GDR FRFLCOLDEPTH_FRCOLDEPTHM_% RosBREED Biswas et al. 2019 LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW University of Delaware FRDEV_FRDEVM_days USDA Chandra et al. 2021 University of Florida SCG_SCGWKM_R-Scategory intermediate 5 Capocasa et al. 2008 Università Politecnica delle Marche FRFIRM_PENEM_g GDR RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale RosBREED 1 calyx size smaller than fruit diameter RosBREED GDR FRFIRM_FRFIRMM_1-9FRFIRMscale RVD_RVD1-3M_1-3RESISTscale Vining, et al. Oregon State UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale 1 sunken CALYXSZ_CALYXSZM_1-3CALYXSZscale RosBREED GDR medium number of flowers 5 8 highly susceptible/severe symptoms USDA FRSHAPE_FRSHAPEGGM_TXT USDA BOTRYTIS_RBCM_0-1BOTRYTISscale CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale RosBREED GDR USDA RMaPh_ RMaPh%M_ % Zurn, et al. stamens taller than receptacle 9 FLPETALNUM_FLPETALNUMM_average USDA USDA FRYIELD_FRYIELDTWM_g FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale CPBR highly susceptible/severe symptoms 9 long conic 2 UPOV FLDIA_FLDIAM_1-9FLDIAUPOVscale Capocasa et al. 2008 SKINCOL_REFLECTLM_L* Università Politecnica delle Marche calyx size equal to fruit diameter 2 RPCCR_RPCCR1-8M_1-8RESISTscale NCRI Eikemo, et al. USDA TITA_TITA0.5M_TITAMeq/100gCA STEMBREAK_PEDBRKM_text USDA light red 7.0 reniform 1 dark 1 TRUSSSIZ_TRUSSM_1-9scale UPOV University of California, Davis RVD_RVD1-9M_1-9RESISTScale Pincot et al. 2020 USDA FRUITSET_FRSETM_1-9FRUITSETscale FRAP_FRAPFSHM_FSHEQumoles/g fruit Università Politecnica delle Marche Capocasa et al. 2008 RosBREED GDR STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale highly susceptible/severe symptoms 1 RUNNERNUM_RUNNERNUMCT_count USDA DAU_RUNDAUM_count USDA completely uniform/symmetrical and attractive 9 level with surface 3 USDA ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq Capocasa et al. 2008 Università Politecnica delle Marche TITA_TITAM_TITAMeqNAOH/100g RosBREED GDR CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale Capocasa et al. 2008 SKINCOL_REFLECTCIXM_ChromaIndex Università Politecnica delle Marche USDA RTL_RUNTOTLENM_m 3 downwards 1 upwards strongly uneven 3 RosBREED GDR ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq RDE_REDM_1-9RESISTscale USDA 4 no fruit set early 3 2 divides at 25% from crown RPOAP_RPOAP%M_% USDA Salinas et al. 2019 University of Florida FRRAFR_FRAFR%M_% Stem breaks just above calyx, good for fresh market Y 2 level with fruit UPOV CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale USDA EPILGT_EPILGTM_cm 3 calyx attachment is even with fruit RosBREED CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale GDR RosBREED GDR PPMF_PPMFM_0-1PMFscale 2 slightly below USDA RMH_RMHM_1-5RMHscale Pinkerton & Finn attribute 1 very soft very small to small 2 3 fully remontant USDA FRpH_pH2M_pH 5.0 globose-conic globose 3 3 low density fully reflexed 9 GDR FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale RosBREED PLOIDY_FCDAPIICFM_x USDA SKINCOL_SKINCOL1-9M_1-9SKINCOLscale RosBREED GDR FRFRESHWT_FRFRESHWT5M_g RosBREED GDR above surface 4 ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale RosBREED GDR FRYIELD_FRYIELD1-4M_1-4FRYIELDscale USDA 2 slightly uneven 1 even 2 slightly below surface very light brown or green 9 FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale UPOV 1 no fruit set PLOIDY_FCDAPIIBLM_x USDA pedicle does not break 1 ECOGDDREQH_GDDHM_days RosBREED GDR 1 soft USDA FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale 5FRW_FRFRESHWT5LM_g USDA 3 slightly uneven 9 hundreds USDA FRRP_FRRPM_Ratio RPOAP_RPOAP1-9M_1-9RESISTscale USDA 4 medium orange upright 1 1 highly susceptible/severe symptoms 5 strongly uneven 7 high globose 4 9 dark red USDA FRCOL1_FRCOL1M_date 3 red FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale UPOV 3 raised USDA PLANTHT_PLANTHTM_cm extremely vigorous 9 RPF_RPF%M_% USDA RPCCR_RPCCR1-5M_1-5RESISTscale UC Davis Jiménez, et al. USDA TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq GDR SSC_SSCM_SSCg/100g RosBREED RosBREED GDR FRYIELD_FRYIELD1-9M_1-9FRYIELDscale 1 divides close to crown SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale UPOV very firm 9 RMYFR_RMYFRM_% USDA blackish red 9 DNARATIO_DNADAPIICFM_DNA/stdDNAILF USDA conic-wedge 3 3.0 dead UPOV FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale 1 no fruit HD_FIRSTHARVM_date USDA 4 divides 75% from crown few 3 strongly above 5.0 1.5 slightly symptomatic divides 50% from crown 3 USDA CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale entity STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale USDA 1 absent or very weak USDA STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale partially remontant 2 dark red 9.0 very resistant, healthy 1.0 USDA FLDATE1_FLDATE1M_FLDATE1date 3 small R resistant USDA Zurn, et al. RFOF_RFOF%M_% white 1 3 protruding RosBREED BEARINGTYPE_BEARINGRBM_count GDR USDA PLANTHABIT_PLHABM_1-9PLHABscale blackish red 10.0 very sweet, no off-flavors 9 2 even medium 5.0 1 spreading cordiform 7 RosBREED GDR SMYFR_SMYFRM_1-9SUSCEPscale 1 no fruit PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale USDA stamens shorter than receptacle 1 5 medium strongly below 1 outwards 2 many flowers 7 9.0 wedged 9 tough 9 complete fruit set pedicle breaks just above calyx 5 UC Davis RFOF_RFOF1-5M_1-5RESISTscale Pincot, et al. 1 dull 6.0 fingerling GDR FRpH_pHM_pH RosBREED same level 3 NEM-RF_RPPM_Rf RPP_RPPM_Rf Pinkerton & Finn USDA 7 pedicle breaks well above calyx FRAP_FRAPTROM_TroloxEQumoles/g FW Capocasa et al. 2008 Università Politecnica delle Marche 2 medium density 9.0 very high GDR SDE_SDEM_1-9SUSCEPscale RosBREED light orange 3 N stem does not break, berry separates from calyx easily 3 low stem breaks well above calyx Y** 7.0 large GDR TotalPhenolics_F-CM_mg/L_GallicAcidEQ RosBREED EVERBERIN_EVER0-1M_0-1EVERscale USDA 9 very glossy 1 very small 1 dead very large 9.0 4 slightly above 2 yellow day neutral 4 1 conic greenish 1 TREXPOS_TREXPOS0-1M_0-1TREXPOSscale RosBREED GDR CPBR RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale conic 2 rhombic 6.0 2.0 mildly symptomatic 2.5 very symptomatic high density 1 very easily removed 9 9.0 very late does not remove 1 inserted 1 1 pinkish white 1 highly resistant/no symptoms not remontant 1 2 medium yield calyx size larger than fruit diameter 3 no flowers 1 highly resistant/no symptoms 9 acronym low yield 3 plant has stamens 9 plant has no stamens 1 V variable, no consistent breaking habit 3 spreading ovate 4 calyx attachment raised above shoulder (necked) 1 1 not reflexed 2 yellowish white very early 1 5.0 divides right before fruit strongly below surface 1 Plant habit The degree of erectness of the leaves. habit PLANTHABIT Plant The toughness of skin. toughness Skin SKINTOUGH Skin toughness CALYXSZ Calyx size size Calyx The size of the calyx. Plant resistance to Verticillium dahliae wilt RVD The plant resistance to verticillium wilt caused by Verticillium dahliae. resistance to Verticillium dahliae wilt Plant 1-10 SKINCOL UPOV scale The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. Plant Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides SCG DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. Resistance to Meloidogyne hapla method Resistance to Phytophthora cactorum CR % method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. mg/L Cy-3-gluc Eq Trolox EQ μmoles/g FW Fruit The weight of fresh fruit. Fruit fresh weight FRFRESHWT fresh weight 1-5 SUSCEP scale color Skin color on the sun side of a fully ripe fruit. Skin color SKINCOL Skin Harvest date method Record the Julian date when first fruit ripens at a specific location. Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. Susceptibility to Phomopsis obscurans method The plant susceptibility to powdery mildew caused by Podosphaera aphanis. SPOAP susceptibility to Podosphaera aphanis Plant Plant susceptibility to Podosphaera aphanis powdery mildew First runner date method Observe the plant and record the Julian date the first runners emerge at a specific location. 1-9 FRSHAPUNI GRIN scale Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method 1-9 FRYIELD scale Bloom date first date first Julian date of first open flowers for an individual, whole plant, or population. Flower BDF Resistance to Macrophomina phaseolina method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Fruit flesh color FRFLCOL flesh color The color of fruit flesh. Fruit GG scale ACHENEVIABLE Achene Percentage of viable achenes viable The percentage of achenes that are viable. Everbearing flowering method Observe the presence of flowers during each week in July, August and September. Estimate fruit yield and record it. Fruit yield 1-9 method Plant fruit development fruit development ECOGDDREQH Plant Growing degree days to first harvest. Observe the truss location relative to the foliage canopy and record it. Truss exposure 0-1 method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Resistance to Verticillium dahliae 1-3 method ferric reducing antioxidant power Fruit ferric reducing antioxidant power FRAP Ferric reducing antioxidant power of fruit. Fruit Plant resistance to Fusarium oxysporum f. sp. fragariae wilt The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. resistance to Fusarium oxysporum f. sp. fragariae wilt Plant RFOF 1-3 CALYXPOS UPOV scale Plant resistance to Phytophthora fragariae red stele root rot resistance to Phytophthora fragariae red stele root rot RPF Plant The plant resistance to red stele root rot caused by Phytophthora fragariae. Fruit The firmness of fruit flesh. flesh firmness FRFIRM Fruit flesh firmness Presence of powdery mildew on fruit method Observe the presence of powdery mildew on ripe fruit. Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-5 method Susceptibility to Verticillium dahliae 1-5 method Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. 1-9 FRSHAPE UPOV scale 1-9 RUNNERNUM scale PLANTVIGOR vigor Plant vigor The vigor of plant. Plant g/100g Leaf pubescence LFPUBESC Leaf pubescence The density of trichomes on the leaf surface. shape uniformity Fruit shape uniformity. FRSHAPEUNIF Fruit shape uniformity Fruit 1-5 RESIST scale Plant resistance to Meloidogyne hapla root knot nematode RMH Plant The plant resistance to root knot nematode Meloidogyne hapla. resistance to Meloidogyne hapla root knot nematode The glossiness of skin. Skin gloss SKINGLOSS Skin gloss FRpH Fruit The pH of fruit. Fruit pH pH bearing type Plant BEARINGTYPE Plant bearing type Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. breaking habit Pedicle breaking habit PEDICLEBREAK Pedicle breakage relative to the calyx. Pedicle 1-9 FRSHAPE RB scale Stem length of primary fruit from calyx to truss. PEDICELLEN Pedicle Pedicle length length Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae % method The effeciency of fruit set at a specific location. FRUITSET Fruit set set Fruit susceptibility to Phomopsis obscurans leaf blight Plant Plant susceptibility to Phomopsis obscurans leaf blight The plant susceptibility to leaf blight caused by Phomopsis obscurans. SPO Number of runners 1-9 method Observe and rate the number of runners per plant. 0-1 CALPLUG scale The plant resistance to crown rot caused by Phytophthora cactorum. Plant resistance to Phytophthora cactorum crown rot Plant resistance to Phytophthora cactorum crown rot RPCCR Fruit Number of days from flowering to ripe fruit. Fruit development FRDEV development Number of flowers per truss estimation Observe the number of flowers per inflorescence. The length of peduncle. Peduncle length Peduncle length PEDUNCLEN Total anthocyanins TACY Fruit The total anthocyanins of fruit. total anthocyanin content Titratable acidity 0.5 g citric acid method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. Fruit fresh weight method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. Count the number of petals on 10 flowers and average the results Petal number average method Fruit yield 1-9 O method Estimate fruit yield and record it. shape Fruit shape The shape of fruit. Fruit FRSHAPE Reflexion of the calyx relative to the fruit shoulders. Calyx CALYXREFLX Calyx reflexion reflexion Fruit Depth of fruit flesh color. FRFLCOLDEPTH flesh depth of color Fruit flesh depth of color DNA ration determination using DAPI with internal standard = iceberg lettuce method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. count FLDATE100 date Plant The number of sets of chromosomes in the nucleus. ploidy PLOIDY Plant ploidy DNA/std DNA_ILF Resistance to Phytophthora cactorum CR 1-5 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. DNA ratio Ratio of sample DNA to standard DNA. DNARATIO Plant Plant DNA ratio Resistance to Colletotrichum acutatum anthracnose fruit rot % method Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. The postion of achene relative to the fruit surface. Achene position ACHENEPOS position Achene Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Fruit flesh color method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Cy-3-gluc method Fruit yield total weight method Weigh total fruit yield and record it. Observe fruit appearance from not uniform to completely uniform. Fruit appearance method date Luminance The weight of fresh fruit. fresh weight largest Fruit Largest fruit weight LFRW Observe the plant and record the relative flowering of the first open flowers. Flower date first relative method Observe where the pedicle breaks during harvest. Pedicle breakage method 1-3 FRCOLUNI scale Observe and rate average fruit skin gloss on 3-5 fruit per genotype. Skin gloss method The total number of daughter plantlets per plant harvested on a single day at a specific location. Runner DAU Runner daughters daughters Record the Julian date when first fruit ripens at a specific location. Fruit harvest date HARVDATE Fruit harvest date FLDIA The diameter of flower at fully open. Flower Flower diameter diameter Total antioxidant content of leaves. Leaf Leaf total antioxidant content total antioxidant content LFTOTANTIOX Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae 0-5 method Observe the truss location relative to the foliage canopy and record it. Truss exposure 1-5 method DNA/std DNA_IBL Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Plant resistance to Phytophthora cactorum root rot The plant resistance to root rot caused by Phytophthora cactorum. Plant resistance to Phytophthora cactorum root rot RPCRR resistance to Diplocarpon earlianum leaf scorch RDE The plant resistance to leaf scorch caused by Diplocarpon earliana. Plant resistance to Diplocarpon earlianum leaf scorch Plant The fruit yield of one individual. FRYIELD fruit yield Plant fruit yield Plant Fruit firmness method Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. Skin color 1-10 method Observe and rate the fruit skin color. Meq/100g citric acid 1-7 RUNNERNUM scale Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Titratable acidity citric acid method Observe fruit color uniformity from even or very slighly unevento strongly uneven. Color uniformity method Whether or not the plant has an everbearing habit Plant bearing type Everbearing EVERBERIN Chroma Index FSH EQ μmoles/g fruit Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Fruit flavor method Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. Depth of fruit flesh color Flower diameter UPOV method Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina % method g Skin toughness method Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Measure the tallest trifoliate leaf from crown to base of leaflets. Plant height method Harvest date HD harvest date Record the Julian date when first fruit ripens at a specific location. Fruit Plant resistance to Mycosphaerella fragariae leaf spot Plant resistance to Mycosphaerella fragariae leaf spot The plant resistance to Mycosphaerella fragariae. RMYFR 1-4 ACHENEPOS UPOV scale FLDATE2 Julian date of last flowering for an individual, whole plant, or population. date last Flower date last Flower Fruit shape GG method Observe and rate average fruit shape on 5 fruit per genotype. R-S category Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Resistance to Fusarium oxysporum f. sp. fragariae % method Record the number of weeks of flowering after week 11 from the first week of bloom. Perpetual flowering RB method Susceptibility to Mycosphaerella fragariae method Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. Plant resistance to Verticillium dahliae Plant resistance to Verticillium dahliae wilt The plant resistance to verticillium wilt caused by Verticillium dahliae. RVD FLEVAL development Flower Weekly flower evaluation The weekly development of flowers. Fruit fresh weight 1 method Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit length FRLEN Fruit Length of fruit from calyx to tip. length Resistance to Verticillium dahliae % method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Fruit fresh weight 5L method Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. RPOAP resistance to Podosphaera aphanis powdery mildew Plant resistance to Podosphaera aphanis powdery mildew The plant resistance to powdery mildew caused by Podosphaera aphanis. Plant total anthocyanin content ATCYN Fruit The total anthocyanins of fruit. Fruit total anthocyanin content Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days harvest method Flower date 100% FLDATE100 Flower Julian date of peak flowering for an individual, whole plant, or population. date 100% Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-3 method Resistance to Podosphaera aphanis % method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Soluble solids method Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. Ratio Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-4 method Observe plant vigor of the two clones and record the average. Plant vigor method 1-3 RESIST scale 1-3 ACHENEPOS scale 1-8 RESIST scale 1-3 ACHENECOL UPOV scale Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Resistance to Mycosphaerella fragariae method Growing degree days to first flowering. Plant ECOGDDREQ flower development Plant flower development % Observe presence of anthers and record it. Presence of stamens 0-1 method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. Fruit pH method days 1-9 FRFLAV scale The Julian date of appearance of the first runners. First runner date RUNNERDATE first emergence Runner Ferric reducing antioxidant power FSH method Plant resistance to necrosis caused by Neopestalotiopsis sp. Plant resistance to necrosis caused by Neopestalotiopsis sp. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). The plant resistance to necrosis caused by Neopestalotiopsis sp. RNEOP 1-3 CALYXREFLX UPOV scale titratable acidity Fruit titratable acidity The titratable acidity content of fruit. TITA Fruit color The color of achene on the sun side of a fully ripe fruit. ACHENECOL Achene color Achene uniformity of color Fruit Fruit uniformity of color. Fruit uniformity of color FRCOLORUNIF Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Skin color chromameter reflect chroma index method Susceptibility to Colletotrichum gloeosporioides weekly method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. Measure fruit firmness in grams using a hand-held penetrometer with a piston. Fruit firmness penetrometer method cm Stem breaking Pedicle STEMBREAK breaking habit Pedicle breakage relative to the calyx. ripening trait FRRP Fruit ripening trait Fruit The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA 1-9 CALYXREM scale 0-1 STAMPRES scale 1-9 ACHENECOL scale Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx plug method Presence of stamens 1-9 method Observe presence of anthers and record it. Daughter plantlet count Count the total number of daughter plants from a single plant harvested on a single day. Calyx position 1-3 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. SSC:TA method mg/L Pg-3-gluc kg−1 Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Perpetual flowering method Resistance to Phytophthora cactorum RR% method Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. 1-9 FLDATE1REL UPOV scale Fruit fresh weight largest method Harvest and record the largest fruit over the harvest season. Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. Calyx size method Fruit Fruit titratable acidity The titratable acidity content of fruit. titratable acidity TITA Truss size Number of flowers per truss Truss size TRUSSSIZ Resistance to Botrytis cinerea method Observe the presence of grey mold on the plant or fruit. Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Crop estimate method Estimate fruit yield and record it. Fruit yield 1-4 method Peduncle length method Observe average peduncle length and record it. 1-9 SKINCOL scale SKINCOL Skin Skin color color Skin color on the sun side of a fully ripe fruit. Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Drip loss method The total length of runners from a single plant harvested on a single day at a specific location. length total Runner Runner total length RTL Resistance to Podosphaera aphanis 1-9 method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Height of tallest trifoliate leaf. Plant height height PLANTHT Plant Ease of calyx removal method Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. 1-5 PEDUNCLEN scale method_of Achene position 1-3 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Plant fruit yield Crop estimate CE The amount of harvested fruit per plant per season. Pedicel measurement method Measure the length of the pedicel of the primary fruit from the calyx to the truss. FLDATE1 Date Percent weight lost when frozen berries are thawed. DRIP LOSS Fruit drip loss Fruit drip loss First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. Susceptibility to Phytophthora fragariae method 1-9 FRFLCOL scale 1-9 SKINTOUGH scale Record the number of days from first flowering to ripe fruit. Fruit development method The plant resistance to leaf blight caused by Phomopsis obscurans. RPO resistance to Phomopsis obscurans leaf blight Plant resistance to Phomopsis obscurans leaf blight The flavor of fruit. Fruit FRFLAV Fruit flavor flavor Measure the length of the epipodium of the primary fruit from the calyx to the truss. Epipodium measurement method 1-9 FRFIRM scale FLDATE2 date The firmness of fruit flesh. Fruit flesh firmness FRFIRM Fruit flesh firmness Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Skin color 1-9 method variable_of Observe the plant and record the date when at peak flowering. Flower date 100% method ease of removal Calyx Ease of calyx removal. Calyx ease of removal CALYXREMOVE The presence of grey mold caused by Botrytis cinerea on the plant or fruit. Indication of resistance to Botrytis Plant presence of grey mold BOTRYTIS 0-5 SUSCEP scale 1-4 PERPFL UPOV scale Stamen height Stamen height The height of the stamens relative to the receptacles. STAMENHGHT Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. Susceptibility to Podosphaera aphanis method Meq NaOH/100g Number of runners count method Harvest and count the number of runners collected from a single crown on a single day in a specific location. Flower date first method Observe the plant and record the date the first flowers open. Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Skin color chromameter reflect L* brightness method Epipodium Stem length of primary fruit from calyx to truss. length EPILGT Epipodium length Folin-Ciocalteu method Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). 0-1 EVER scale 0-6 RESIST scale 0-1 PMF scale 1-9 FRGLOSS scale 1-3 PLHAB scale mg/L Gallic Acid EQ 1-9 CALYXREFLX scale Percentage of viable achenes method Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. Plant resistance to Pratylenchus penetrans root lesion nematode resistance to Pratylenchus penetrans root lesion nematode RPP The plant resistance to root lesion nematode Pratylenchus penetrans. Plant Stembreak txt 1-9 RESIST scale 1-9 FRUITSET scale 0-1 FLEVAL scale 1-5 FRCOLUNI scale Plant susceptibility to Verticillium dahliae wilt Plant SVD The plant susceptibility to verticillium wilt caused by Verticillium dahliae. susceptibility to Verticillium dahliae wilt Plant susceptibility to Mycosphaerella fragariae leaf spot SMYFR The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. Plant susceptibility to Mycosphaerella fragariae leaf spot Flower date last method Observe the plant and record the date when at the end of flowering. first color appearance Fruit Appearance of first color on fruit. FRCOL1 Appearance of first color on fruit Resistance to Neopestalotiopsis sp. scale 0-6 Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants The total phenolic acids of fruit. total phenolic acids content Fruit TPHE Fruit total phenolic acids content 1-9 PLHAB scale Plant susceptibility to Phytophthora fragariae red stele root rot The plant susceptibility to red stele root rot caused by Phytophthora fragariae. SPF Plant susceptibility to Phytophthora fragariae red stele root rot exposure Truss TREXPO Truss exposure The position of the truss relative to the foliage. Observe the average achene color on 3-5 fruit per genotype and record it. Achene color 1-9 method Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Resistance to Pratylenchus penetrans number of nematodes/g root method Flower date first Julian date of first open flowers for an individual, whole plant, or population. Flower date first FLDATE1 x The plant resistance to charcoal rot caused by Macrophomina phaseolina. resistance to Macrophomina phaseolina charcoal rot Plant resistance to Macrophomina phaseolina charcoal rot Plant RMaPh 5FRW fresh weight Fruit Fruit weight The weight of fresh fruit. Flower diameter method Measure the diameter of the flowers at fully open 1-9 FRYIELD Ordinal scale Reproductive factor scale_of Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Resistance to Verticillium dahliae scale 1-9 grams average Fruit shape RB method Observe and rate average fruit shape on 3-5 fruit per genotype. 1-5 TREXPOS UPOV scale 1-9 SUSCEP scale 1-9 PLANTVIGOR scale Runner number Runner RUNNERNUM The number of runners. number Plant SDE susceptibility to Diplocarpon earliana Plant susceptibility to leaf scorch caused by Diplocarpon earliana The plant susceptibility to leaf scorch caused by Diplocarpon earliana Fruit The presence of powdery mildew on fruit. PPMF Fruit presence of powdery mildew presence of powdery mildew Observe and rate the height of the stamens relative to the receptacle at flower opening. Stamen height method Fruit The soluble solids content of fruit. SSC total water soluble solid content Fruit total water soluble solid content Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method presence Stamen Stamen presence The presence of stamens. STAMENPRES 1-9 STAMENHGHT scale 1-7 PEDBRK scale Harvest all runners on a single day and measure the total length. Runner total length method Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Resistance to Diplocarpon earlianum method meters 1-5 CALYXPOS scale FRpH Observe the plant and assess the erectness or spreading of the leaves along the ground. Plant habit scale method 1-9 FLDIA UPOV scale 1-3 pubescence scale Ferric reducing antioxidant power trolox method Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). 1-4 FRYIELD scale resistance to Colletotrichum acutatum anthracnose fruit rot Fruit resistance to Colletotrichum acutatum anthracnose fruit rot Fruit The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. RAFR Calyx The position of the calyx attachment relative to the fruit shoulder. position CALYXPOS Calyx position 0-1 BOTRYITIS scale 0-1 TREXPOS scale Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Fruit pH 2 method FLDATE1REL Flower date first relative date first relative Flower Relative date of first open flowers for an individual, whole plant, or population. 1-5 RMH scale mm 0-7 TRUSS scale Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. Leaf total antioxidant assay method Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. Fruit set method Observe the number of fruit that a single plant sets in a specific location. Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape UPOV method petal number Average flower petal number. Flower FLPETALNUM Flower petal number 1-9 STAMPRES scale Weekly flowering evaluation Observe the plant weekly and record if it is flowering. Fruit ripen days Fruit development FRD Number of days from flowering to ripe fruit. Observe the plant and record the reflexion of the calyx relative to the fruit shoulders Calyx reflexion method Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Susceptibility to Diplocarpon earlianum method Presence or absence of central plug after calyx removal. Calyx plug Calyx plug CALYXPLUG Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. 1-3 CALYXSZ scale Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Growing degree days flowering method Measure the length of the fruit from the calyx to the tip and record in mm. Fruit length method Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Titratable acidity NaOH method Number of runners 1-7 method Observe and rate the number of runners per plant. Total anthocyanins Pg-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5.