UPOV CALYXPOS_CALYXPOS1-3M_1-3CALYXPOSUPOVscale GDR RosBREED PEDUNCLEN_PEDUNCLENM_1-5PEDUNCLENscale CPBR PLANTHABIT_PLHABM_1-3PLHABscale USDA FRUITSET_FRSETM_1-9FRUITSETscale medium red 8.0 Biswas et al. 2019 University of Delaware LFTOTANTIOX_LFTOTANTIOXM_TroloxEQumoles/g FW FRYIELD_FRYIELD1-9OM_1-9FRYIELDOscale UPOV 1 not remontant RosBREED GDR SMYFR_SMYFRM_1-9SUSCEPscale 3 spreading GDR RosBREED CALYXPOS_CALYXPOS1-5M_1-5CALYXPOSscale TITA_TITAM_TITAMeqNAOH/100g Capocasa et al. 2008 Università Politecnica delle Marche USDA RUNNERNUM_RUNNERNUMCT_count 3 low yield 9 very glossy 8.0 circular TRUSSSIZ_TRUSSCOUNTM_ave GDR RosBREED oblong 5.0 USDA TITA_TITA0.5M_TITAMeq/100gCA ACHENECOL_ACHENECOL1-3M_1-3ACHENECOLUPOVscale UPOV RDE_REDM_1-9RESISTscale USDA GDR CALYXPLUG_CALYXPLUGM_0-1CALPLUGscale RosBREED ECOGDDREQ_GDDFM_days RosBREED GDR slightly uneven 3 USDA FRCOL1_FRCOL1M_date USDA HD_FIRSTHARVM_date 9 very easily removed 8.0 large to very large GDR TREXPOS_TREXPOS0-1M_0-1TREXPOSscale RosBREED USDA RPOAP_RPOAP%M_% 1 very small globose 3 GDR ACHENEPOS_ACHENEPOS1-3M_1-3ACHENEPOSscale RosBREED CE_CROPEST_g USDA dull 1 RosBREED FRpH_pHM_pH GDR University of Florida Salinas et al. 2019 FRRAFR_FRAFR%M_% 1 white 9 plant has stamens 3 few flowers 1 dead strongly below surface 1 GDR RosBREED SKINGLOSS_SKINGLOSSM_1-9GLOSSscale sunken 1 USDA DAU_RUNDAUM_count RosBREED FRFRESHWT_FRFRESHWT5M_g GDR RosBREED GDR ECOGDDREQH_GDDHM_days 1 calyx size smaller than fruit diameter 5 highly susceptible/severe symptoms 2 medium density GDR RosBREED TotalPhenolics_F-CM_mg/L_GallicAcidEQ 2 very small to small 9 extremely vigorous USDA FRRP_FRRPM_Ratio RPO_RPOM_% USDA SKINCOL_REFLECTLM_L* Università Politecnica delle Marche Capocasa et al. 2008 1 reniform semi-upright 2 USDA FRD_FRRIPEM_days RosBREED GDR SDE_SDEM_1-9SUSCEPscale 1 not sweet, bad off-flavors USDA FLDATE1_FLDATE1M_FLDATE1date Fernandez et al. University of North Carolina RNEOP_RNEOP0-6M_0-6RESISTscale 5 medium number of flowers BEARINGTYPE_BEARINGM_1-4PERPFLUPOVscale UPOV USDA STAMENPRES_STAMENPRES1-9M_1-9STAMPRESscale 4 globose V variable, no consistent breaking habit USDA FRFRESHWT_FRFRESHWTLM_FRFRESHWT1Mg GDR FRFLCOL_FRFLCOLM_1-9FRFLCOLscale RosBREED RPCCR_RPCCR1-8M_1-8RESISTscale NCRI Eikemo, et al. FRYIELD_FRYIELD1-9M_1-9FRYIELDscale RosBREED GDR RPF_RPF0-5M_0-5RESISTscale GDR RosBREED RFOF_RFOF%M_% USDA Zurn, et al. UPOV FRSHAPE_FRSHAPEUPOVM_1-9FRSHAPEUPOVscale RosBREED SSC_SSCM_SSCg/100g GDR USDA FRYIELD_FRYIELD1-4M_1-4FRYIELDscale USDA CALYXREFLX_CALYXREFLXM_1-9CALYXREFLXscale RosBREED SPOAP_SPOAPM_1-9SUSCEPscale GDR FLDIA_FLDIAM_cm UPOV FRSHAPEUNIF_FRSHAPEUNIFM_1-9FRSHAPUNIGRINscale USDA CALYXREMOVE_CALYXREMM_1-9CALYXREMPscale GDR RosBREED STAMENHGHT_STAMENHGHTM_1-9STAMENHGHTscale USDA Biswas et al. 2019 University of Delaware LFPUBESC_LFPUBESCM_1-3LFPUBESCscale 9 tough CPBR RUNNERNUM_RUNNERNUM1-7M_1-7RUNNERNUMscale USDA FLPETALNUM_FLPETALNUMM_average GDR BEARINGTYPE_BEARINGRBM_count RosBREED RosBREED ACHENEVIABLE_ACHVIAM_% GDR entity 3 breakage is inconsistent 5 pedicle breaks just above calyx Capocasa et al. 2008 SKINCOL_REFLECTCIXM_ChromaIndex Università Politecnica delle Marche STEMBREAK_PEDBRKM_text USDA very light brown or green 9 PLOIDY_FCDAPIICFM_x USDA Università Politecnica delle Marche Capocasa et al. 2008 FRAP_FRAPTROM_TroloxEQumoles/g FW GDR PPMF_PPMFM_0-1PMFscale RosBREED USDA 5FRW_FRFRESHWT5LM_g 1 divides close to crown USDA RMaPh_ RMaPh%M_ % Zurn, et al. RosBREED GDR ACHENECOL_ACHENECOL1-9M_1-9ACHENECOLscale USDA EVERBERIN_EVER0-1M_0-1EVERscale FRLEN_FRLENM_mm USDA 7.0 large acronym UC Davis RFOF_RFOF1-5M_1-5RESISTscale Pincot, et al. attribute UC Davis SVD_SVD1-5M_1-5SUSCEPscale Feldmann, et al. FLEVAL_FLEVALM_0-1FLEVALscale GDR RosBREED pedicle does not break 1 RosBREED GDR ATCYN_ATCYNP3GM_ATCYNmg/L_Pg-3-glucEq GDR RosBREED FRSHAPE_FRSHAPERBM_1-9FRSHAPERBscale GDR RUNNERNUM_RUNNERNUM1-9M_1-9RUNNERNUMscale RosBREED orange red 5.0 USDA RUNNERDATE_FRUNNERM_date UC Davis RMaPh_ RMaPhM_1-5RESISTscale Knapp, et al. GDR RosBREED STAMENPRES_STAMENPRES0-1M_0-1STAMPRESscale 4 ovate DNARATIO_DNADAPIICFM_DNA/stdDNAILF USDA RosBREED PLANTVIGOR_PLANTVIGORM_1-9PLANTVIGORscale GDR RPF_RPF%M_% USDA Università Politecnica delle Marche Capocasa et al. 2008 FRFIRM_PENEM_g USDA BLDATE1_FLDATE1M_FLDATE1date 1 highly resistant/no symptoms 4 above surface same level 3 divides 75% from crown 4 USDA RPOAP_RPOAP1-9M_1-9RESISTscale fingerling 6.0 1 very early Università Politecnica delle Marche Capocasa et al. 2008 FRAP_FRAPFSHM_FSHEQumoles/g fruit partially remontant 2 Jiménez, et al. RPCCR_RPCCR1-5M_1-5RESISTscale UC Davis 3 level with surface small to medium 4 5.0 strongly above USDA FRCOLORUNIF_FRCOLORUNIFM_1-5FRCOLUNIscale GDR FRFLCOLDEPTH_FRCOLDEPTHM_% RosBREED 1 not uniform/malformed many 7 UPOV FLDATE1REL_FLDATE1RELM_1-9FLDATE1RELUPOVscale RPCRR_RPC%M_% USDA RosBREED GDR TITA_TITAM_TITAMeq/100gCA 5 intermediate USDA FRYIELD_FRYIELDTWM_g GDR DRIPLOSS_DRIPLOSSM_g RosBREED Y Stem breaks just above calyx, good for fresh market very resistant, healthy 1.0 PEDICLEBREAK_PEDBRKM_1-7PEDBRKscale USDA medium 5.0 RVD_RVD%_% USDA FLDATE2_FLDATE2M_date USDA RPP_RPPM_Rf USDA Pinkerton & Finn NEM-RF_RPPM_Rf CPBR FRCOLORUNIF_FRCOLORUNIFM_1-3FRCOLUNIscale PLANTHABIT_PLHABM_1-9PLHABscale USDA 1 highly resistant/no symptoms divides at 25% from crown 2 UPOV TREXPOS_TREXPOS1-5M_1-5TREXPOUPOVscale 5 strongly uneven 3 strongly uneven 7.0 light red USDA PLOIDY_FCDAPIIBLM_x R resistant SCG_SCGWKM_R-Scategory Chandra et al. 2021 University of Florida USDA ATCYN_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq 1 no fruit 1 not reflexed highly resistant/no symptoms 1 USDA FRSHAPE_FRSHAPEGGM_TXT soft 1 light orange 3 upright 1 9 hundreds high density 1 CALYXSZ_CALYXSZM_1-3CALYXSZscale RosBREED GDR FRDEV_FRDEVM_days USDA USDA FLDATE100_FLDATE100M_FLDATE100date 7 late RosBREED SPO_SPOM_1-9SUSCEPscale GDR HARVDATE_HARVDATEM_date RosBREED GDR RosBREED SKINTOUGH_SKINTOUGHM_1-9SKINTOUGHscale GDR 5 calyx attachment is sunken below shoulder EPILGT_EPILGTM_cm USDA 1 spreading 7 high raised 3 FRFLAV_FRFLAVM_1-9FRFLAVscale GDR RosBREED no fruit set 4 1 high yield USDA BOTRYTIS_RBCM_0-1BOTRYTISscale UPOV FLDIA_FLDIAM_1-9FLDIAUPOVscale 1 even or very slightly uneven USDA FRpH_pH2M_pH 1 even 1 pinkish white 3 low USDA PEDICELLEN_PEDICELLENM_cm highly susceptible/severe symptoms 1 1.5 slightly symptomatic 2 slightly uneven 1 conic 3 red USDA RTL_RUNTOTLENM_m calyx size larger than fruit diameter 3 1 no runners highly susceptible/severe symptoms 1 3 fully remontant TACY_ATCYNC3GM_ATCYNmg/L_Cy-3-glucEq USDA no fruit set 1 RMYFR_RMYFRM_% USDA RosBREED SKINCOL_SKINCOL1-9M_1-9SKINCOLscale GDR wedged 9.0 highly susceptible/severe symptoms 9 SPF_SPFM_0-5SUSCEPscale RosBREED GDR globose-conic 5.0 1 does not remove UPOV ACHENEPOS_ACHENEPOS1-4M_1-4ACHENEPOSUPOVscale Pinkerton & Finn USDA RMH_RMHM_1-5RMHscale slightly below 2 very soft 1 1 no flowers level with fruit 2 DNARATIO_DNADAPIIBLM_DNA/stdDNAIBL USDA low density 3 yellow 2 7 many flowers RPCCR_RPCCR%M_% USDA RosBREED FRFIRM_FRFIRMM_1-9FRFIRMscale GDR UPOV CALYXREFLX_CALYXREFLXM_1-3CALYXREFLXUPOVscale 1 inserted 9 stamens taller than receptacle 9.0 oblate Pincot et al. 2020 RVD_RVD1-9M_1-9RESISTScale University of California, Davis divides right before fruit 5.0 9 very firm highly resistant/no symptoms 9 very sweet, no off-flavors 9 SKINCOL_SKINCOL1-10M_1-10SKINCOLUPOVscale UPOV absent or very weak 1 outwards 2 S susceptible pink 6.0 stamens shorter than receptacle 1 very large 9.0 PLANTHT_PLANTHTM_cm USDA 1 white conic 2 3 early dark red 9.0 divides 50% from crown 3 oblate 7.0 medium yield 2 globose-conic 5 USDA LFRW_FRFRESHWLGM_g 9 overcropped 1 dark yellowish white 2 8 highly susceptible/severe symptoms TRUSSSIZ_TRUSSM_1-9scale UPOV dark red 9 2 calyx size equal to fruit diameter blackish red 10.0 1 greenish 9.0 very late 1 calyx attachment raised above shoulder (necked) strongly below 1 3 protruding 2 long conic upwards 1 long conic 1 1 no fruit 7 pedicle breaks well above calyx 9 completely uniform/symmetrical and attractive N stem does not break, berry separates from calyx easily 5 medium 3.0 dead conic-wedge 3 blackish red 9 2.0 mildly symptomatic downwards 3 9.0 very high medium to large 6.0 Oregon State RVD_RVD1-3M_1-3RESISTscale Vining, et al. 4 medium orange 3 few rhombic 6.0 even 2 5 medium 3 cordate 2 slightly below surface 9 fully reflexed complete fruit set 9 Y** stem breaks well above calyx 9 erect 3 calyx attachment is even with fruit 4 slightly above plant has no stamens 1 very symptomatic 2.5 day neutral 4 9 highly resistant/no symptoms Strawberry ontology 3 small 7 cordiform ease of removal Ease of calyx removal. CALYXREMOVE Calyx ease of removal Calyx Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. Flow Cytometry determination using DAPI with internal standard = Ilex crenata Fastigiata method Calyx position 1-5 method Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Growing degree days flowering method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Reproductive factor exposure TREXPO Truss exposure The position of the truss relative to the foliage. Truss 0-1 PMF scale g The flavor of fruit. Fruit Fruit flavor flavor FRFLAV Flower date last FLDATE2 date last Julian date of last flowering for an individual, whole plant, or population. Flower Runner First runner date RUNNERDATE first emergence The Julian date of appearance of the first runners. Fruit harvest date Record the Julian date when first fruit ripens at a specific location. harvest date Fruit HARVDATE Plant The plant resistance to root rot caused by Phytophthora cactorum. Plant resistance to Phytophthora cactorum root rot RPCRR resistance to Phytophthora cactorum root rot 1-3 RESIST scale Observe and rate the susceptibility of the plant to leaf scorch caused by Diplocarpon earlianum. Susceptibility to Diplocarpon earlianum method Plant Evaluation to identify short-day plants that flower only in the spring when day length is less than 14 hrs, and those that continue to flower until the days begin to shorten in late summer. Plant bearing type bearing type BEARINGTYPE Truss size size Truss Number of flowers per truss TRUSSSIZ Observe and rate average fruit shape on 5 fruit per genotype. Fruit shape GG method 1-9 FRGLOSS scale Calyx size size Calyx The size of the calyx. CALYXSZ Observe plant vigor of the two clones and record the average. Plant vigor method 1-5 PEDUNCLEN scale resistance to Verticillium dahliae wilt Plant The plant resistance to verticillium wilt caused by Verticillium dahliae. RVD Plant resistance to Verticillium dahliae wilt 1-9 PLANTVIGOR scale % Stamen height Stamen height The height of the stamens relative to the receptacles. STAMENHGHT length Peduncle The length of peduncle. PEDUNCLEN Peduncle length Pedicle breaking habit Pedicle breakage relative to the calyx. PEDICLEBREAK Pedicle breaking habit Fruit FRCOL1 first color appearance Appearance of first color on fruit. Appearance of first color on fruit RPP resistance to Pratylenchus penetrans root lesion nematode The plant resistance to root lesion nematode Pratylenchus penetrans. Plant Plant resistance to Pratylenchus penetrans root lesion nematode Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis % method FRpH 1-5 TREXPOS UPOV scale Achene position 1-4 method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Observe presence of anthers and record it. Presence of stamens 1-9 method Runner total length method Harvest all runners on a single day and measure the total length. height Height of tallest trifoliate leaf. PLANTHT Plant Plant height 1-3 CALYXPOS UPOV scale TITA Fruit titratable acidity The titratable acidity content of fruit. titratable acidity Fruit Observe presence of anthers and record it. Presence of stamens 0-1 method g/100g Ease of calyx removal method Estimate the average ease of calyx removal by grasping the calyx with fingers and pulling up on 3-5 fruit per genotype. Growing degree days harvest method Record the mean of daily maximum and minimum temperature, minus the base of 10 °C using appropriate weather station data beginning 1 January and accumulated throughout the year. Fruit Fruit ripening trait The ratio of sugar to acid (TSS:TA) calculated as TSS divided by TA ripening trait FRRP Perpetual flowering RB method Record the number of weeks of flowering after week 11 from the first week of bloom. 1-4 ACHENEPOS UPOV scale FRFIRM flesh firmness The firmness of fruit flesh. Fruit flesh firmness Fruit Fruit Fruit flesh depth of color flesh depth of color FRFLCOLDEPTH Depth of fruit flesh color. Count the total number of daughter plants from a single plant harvested on a single day. Daughter plantlet count date first relative Flower Relative date of first open flowers for an individual, whole plant, or population. Flower date first relative FLDATE1REL 0-7 TRUSS scale Fruit development development FRDEV Fruit Number of days from flowering to ripe fruit. Observe and rate the resistance of the plant to root rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum RR% method Calyx reflexion method Observe the plant and record the reflexion of the calyx relative to the fruit shoulders Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR 1-5 method The plant susceptibility to leaf spot caused by Mycosphaerella fragariae. SMYFR Plant susceptibility to Mycosphaerella fragariae leaf spot susceptibility to Mycosphaerella fragariae leaf spot Plant length total RTL Runner Runner total length The total length of runners from a single plant harvested on a single day at a specific location. Susceptibility to Mycosphaerella fragariae method Observe and rate the susceptibility of the plant to leaf spot caused by Mycosphaerella fragariae. 1-3 PLHAB scale 0-1 FLEVAL scale Flower petal number Average flower petal number. Flower FLPETALNUM petal number flesh firmness Fruit Fruit flesh firmness FRFIRM The firmness of fruit flesh. Fruit weight 5FRW fresh weight Fruit The weight of fresh fruit. Flower date 100% Flower FLDATE100 Julian date of peak flowering for an individual, whole plant, or population. date 100% ploidy PLOIDY Plant ploidy The number of sets of chromosomes in the nucleus. Plant Number of flowers per truss count Select 3 flower trusses, count the flowers and average the results. Observe the plant weekly and record if it is flowering. Weekly flowering evaluation Weekly flower evaluation development Flower The weekly development of flowers. FLEVAL Number of runners count method Harvest and count the number of runners collected from a single crown on a single day in a specific location. resistance to Phomopsis obscurans leaf blight Plant resistance to Phomopsis obscurans leaf blight RPO The plant resistance to leaf blight caused by Phomopsis obscurans. 1-3 pubescence scale The diameter of flower at fully open. FLDIA Flower Flower diameter diameter The glossiness of skin. SKINGLOSS Skin gloss Skin gloss 1-4 PERPFL UPOV scale Petal number average method Count the number of petals on 10 flowers and average the results CALYXREFLX Calyx Calyx reflexion Reflexion of the calyx relative to the fruit shoulders. reflexion mm vigor The vigor of plant. Plant vigor PLANTVIGOR Plant FRUITSET set The effeciency of fruit set at a specific location. Fruit Fruit set 1-9 STAMPRES scale FSH EQ μmoles/g fruit Fruit firmness method Compress a fully ripe fruit between thumb and forefinger and rate the average firmness of flesh on 3-5 fruit per genotype. 1-5 CALYXPOS scale Pedicle breakage relative to the calyx. Pedicle breaking habit STEMBREAK Stem breaking 1-9 FRSHAPE UPOV scale Susceptibility to Podosphaera aphanis method Observe and rate the susceptibility of the plant to powdery mildew caused by Podosphaera aphanis. Observe the plant and record the relative flowering of the first open flowers. Flower date first relative method 1-8 RESIST scale Truss exposure 0-1 method Observe the truss location relative to the foliage canopy and record it. Indication of resistance to Botrytis presence of grey mold The presence of grey mold caused by Botrytis cinerea on the plant or fruit. Plant BOTRYTIS Resistance to Verticillium dahliae 1-3 method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. SKINCOL color Skin Skin color Skin color on the sun side of a fully ripe fruit. Pedicel measurement method Measure the length of the pedicel of the primary fruit from the calyx to the truss. FRSHAPEUNIF shape uniformity Fruit shape uniformity. Fruit Fruit shape uniformity Estimate fruit yield and record it. Fruit yield 1-9 method Stem length of primary fruit from calyx to truss. Pedicle length PEDICELLEN Pedicle length Total cumulative mass of ripe fruits from up to four separate harvests between the harvest season at a specific location. Crop estimate method Calculate the ratio of soluble solids to titratable acidity by dividing SSC or TSS by TITA or TA. SSC:TA method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes chroma index, where a high chroma index indicates pale fruit and a low chroma index indicates dark fruit. Skin color chromameter reflect chroma index method Observe and rate the height of the stamens relative to the receptacle at flower opening. Stamen height method Fruit SSC total water soluble solid content The soluble solids content of fruit. Fruit total water soluble solid content Ratio of sample DNA to standard DNA. DNARATIO Plant DNA ratio Plant DNA ratio count Length of fruit from calyx to tip. FRLEN Fruit length length Fruit Observe and rate the number of runners per plant. Number of runners 1-7 method Fruit firmness penetrometer method Measure fruit firmness in grams using a hand-held penetrometer with a piston. CALYXPOS Calyx position Calyx position The position of the calyx attachment relative to the fruit shoulder. Resistance to Macrophomina phaseolina % method Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. The color of achene on the sun side of a fully ripe fruit. color ACHENECOL Achene Achene color 1-9 PLHAB scale Observe the presence of powdery mildew on ripe fruit. Presence of powdery mildew on fruit method 0-5 SUSCEP scale susceptibility to Podosphaera aphanis The plant susceptibility to powdery mildew caused by Podosphaera aphanis. Plant Plant susceptibility to Podosphaera aphanis powdery mildew SPOAP habit Plant habit Plant The degree of erectness of the leaves. PLANTHABIT 1-10 SKINCOL UPOV scale Leaf Leaf pubescence The density of trichomes on the leaf surface. pubescence LFPUBESC Fruit fresh weight largest method Harvest and record the largest fruit over the harvest season. meters Perpetual flowering method Observe the plants and rate based on their flowering duration. Remontantcy is scored as follows: 1) flowering and fruiting only once in a season; 2) the potential to flower and fruit twice in a season, but strongly influenced by the environment; 3) flowering and fruiting twice in a season, largely irrespective of the environment; 4) flowering and fruiting multiple times, continuously in a season. Do not require decreasing day length for flower induction. In cases where stolons are observed, therefore, stolons produce flowers and fruits in the same season as the mother plant. Plant SCG susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides Plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides The plant susceptibiltiy to crown rot caused by Colletotrichum gloeosporioides. Plant habit scale method Observe the plant and assess the erectness or spreading of the leaves along the ground. Flow Cytometry determination using DAPI with internal standard = iceberg lettuce method Flow Cytometry determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae 0-5 method Observe and rate theResistance of the plant to powdery mildew caused by Podosphaera aphanis. Resistance to Podosphaera aphanis 1-9 method days 1-9 FLDATE1REL UPOV scale Measure titratable acidity of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator with 5 grams of puree mixed with 45 ml of CO2-free water. Titrate samples with 0.1 normal NaOH to pH 8.1. Titratable acidity citric acid method RPF The plant resistance to red stele root rot caused by Phytophthora fragariae. Plant resistance to Phytophthora fragariae red stele root rot resistance to Phytophthora fragariae red stele root rot Plant Folin-Ciocalteu method Total phenolics was measured on all fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Fruit was homogenized by rolling a weighted glass bottle over the bag, crushing the fruit and achenes. Homogenate was tested for titratable acid and then 1.5 mL was saved in three 2 mL tubes and stored at −80 °C. Thirty minutes prior to testing, one vial of each of the stored samples was thawed, vortexed and centrifuged. The determination of total phenolics was performed using the Folin-Ciocalteu method as described by Waterhouse (2002) modified for use in 90-well, flat-bottomed microplates (Greiner Bio-One, Monroe, NC, USA). Observe and rate the average positon of the calyx attachment relative to the shoulder of the fruit on 3-5 fruit per genotype. Calyx position 1-3 method Observe the presence of grey mold on the plant or fruit. Resistance to Botrytis cinerea method Fruit yield 1-4 method Estimate fruit yield and record it. RVD The plant resistance to verticillium wilt caused by Verticillium dahliae. Plant resistance to Verticillium dahliae wilt Plant resistance to Verticillium dahliae GG scale The number of runners. number Runner Runner number RUNNERNUM First fruit color method Record the Julian date when first fruit develops ripe color on at least half of its surface fruit yield Plant FRYIELD The fruit yield of one individual. Plant fruit yield variable_of Fruit The titratable acidity content of fruit. titratable acidity TITA Fruit titratable acidity Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. Total anthocyanins Pg-3-gluc method Measure the diameter of the flowers at fully open Flower diameter method Fruit shape The shape of fruit. FRSHAPE Fruit shape Observe and rate the average fruit flesh color on 3-5 fruit per genotype. Fruit flesh color method DRIP LOSS Fruit drip loss drip loss Percent weight lost when frozen berries are thawed. Fruit DNA ration determination using DAPI with internal standard = iceberg lettuce method DNA ratio determination using DAPI with internal standard = iceberg lettuce. Performed by Plant Cytometry Services, The Netherlands. Meq/100g citric acid Plant flower development Plant flower development Growing degree days to first flowering. ECOGDDREQ date first Julian date of first open flowers for an individual, whole plant, or population. Flower BDF Bloom date first 1-4 FRYIELD scale Measure the tallest trifoliate leaf from crown to base of leaflets. Plant height method 1-9 SUSCEP scale Drip loss method Remove frozen fruit samples the night before to fully thaw. Note the number of fruit per bag and weight to calculate average berry weight and percent drip loss. Carefully place the thawed fruit in a clamshell, separating it from the juice without squeezing the fruit, eliminating the release of excess juice or changing the fruit’s integrity. Weigh the juice. Calculate total drip loss as a percentage of fruit weight by the formula: W1-W2/W1 where W1 is the average berry weight before drip loss occurred and W2 is the average berry weight after drip loss. Measure the length of the epipodium of the primary fruit from the calyx to the truss. Epipodium measurement method Resistance to Pratylenchus penetrans number of nematodes/g root method Observe and rate the resistance of the plant to root lesion nematode caused by Pratylenchus prenetrans. Weigh total fruit yield and record it. Fruit yield total weight method Resistance to Verticillium dahliae % method Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae. Plant resistance to necrosis caused by Neopestalotiopsis sp. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as ferrous sulphate heptahydrate (μmoles/g fruit). The plant resistance to necrosis caused by Neopestalotiopsis sp. RNEOP Plant resistance to necrosis caused by Neopestalotiopsis sp. Ferric reducing antioxidant power FSH method Observe and rate the resistance of the plant to red stele root rot caused by Phytophthora fragariae. Resistance to Phytophthora fragariae % method 1-9 SKINTOUGH scale Trolox EQ μmoles/g FW Depth of fruit flesh color Slice the fruit down the meridian, observe and rate the average percentage of solid color in 10% increments on 3-5 fruit per genotype. 1-9 FRFIRM scale Ferric reducing antioxidant power of fruit. FRAP Fruit ferric reducing antioxidant power ferric reducing antioxidant power Fruit Susceptibility to Phytophthora fragariae method Observe and rate the susceptibility of the plant to red stele root rot caused by Phytophthora fragariae. The amount of harvested fruit per plant per season. fruit yield Crop estimate CE Plant ATCYN Fruit The total anthocyanins of fruit. total anthocyanin content Fruit total anthocyanin content toughness Skin The toughness of skin. SKINTOUGH Skin toughness Stem length of primary fruit from calyx to truss. Epipodium EPILGT length Epipodium length 1-9 CALYXREM scale Fruit fresh weight method Harvest and record the average weight of five primary fruit harvested when 30-50% of the fruit on each plant are ripe. The color of fruit flesh. Fruit flesh color flesh color FRFLCOL Fruit DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata. Performed by Plant Cytometry Services, The Netherlands. DNA ratio determination using DAPI with internal standard = Ilex crenata Fastigiata method Plant The plant resistance to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. RFOF Plant resistance to Fusarium oxysporum f. sp. fragariae wilt resistance to Fusarium oxysporum f. sp. fragariae wilt FLDATE1 Date Flower date first method Observe the plant and record the date the first flowers open. 1-9 FRYIELD Ordinal scale Flower date last method Observe the plant and record the date when at the end of flowering. FLDATE2 date Susceptibility to Phomopsis obscurans method Observe and rate the susceptibility of the plant to leaf blight caused by Phomopsis obscurans. 1-9 FLDIA UPOV scale Stembreak txt Fruit shape UPOV method Observe and rate average fruit shape on 3-5 fruit per genotype. Everbearing flowering method Observe the presence of flowers during each week in July, August and September. Observe where the pedicle breaks during harvest. Pedicle breakage method fresh weight FRFRESHWT Fruit fresh weight Fruit The weight of fresh fruit. The plant susceptibility to leaf blight caused by Phomopsis obscurans. Plant Plant susceptibility to Phomopsis obscurans leaf blight SPO susceptibility to Phomopsis obscurans leaf blight Leaf total antioxidant assay method Quantify total antioxidant using an assay kit following manufacture directions. Measure endpoint absorbance at 405 nm using a colorimeter. total phenolic acids content Fruit total phenolic acids content Fruit TPHE The total phenolic acids of fruit. 1-9 FRSHAPUNI GRIN scale Achene color 1-9 method Observe the average achene color on 3-5 fruit per genotype and record it. Measure soluble solids content of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag and using a refractometer. Soluble solids method 1-3 ACHENEPOS scale 1-9 FRFLAV scale Collect ripe fruit from the field and freeze to −18 °C within 24 h and hold for at least 3 days. Postharvest fruit quality data are measured on ripe frozen fruit, using pooled and homogenized five-berry samples. Sample pH is measured using pH meter. Fruit pH 2 method 1-9 RESIST scale mg/L Gallic Acid EQ Fruit resistance to Colletotrichum acutatum anthracnose fruit rot resistance to Colletotrichum acutatum anthracnose fruit rot RAFR Fruit The fruit resistance to anthracnose fruit rot caused by Colletotrichum acutatum. cm Fruit yield 1-9 O method Estimate fruit yield and record it. Fruit pH method Measure pH of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag using an auto titrator. RPOAP The plant resistance to powdery mildew caused by Podosphaera aphanis. Plant Plant resistance to Podosphaera aphanis powdery mildew resistance to Podosphaera aphanis powdery mildew Julian date of first open flowers for an individual, whole plant, or population. Flower Flower date first FLDATE1 date first 1-9 RUNNERNUM scale Observe the plant and record the date when at peak flowering. Flower date 100% method Achene color 1-3 method Observe the average achene color on 3-5 fruit per genotype and record it. x Observe and rate the number of runners per plant. Number of runners 1-9 method RMaPh Plant resistance to Macrophomina phaseolina charcoal rot The plant resistance to charcoal rot caused by Macrophomina phaseolina. Plant resistance to Macrophomina phaseolina charcoal rot 1-5 FRCOLUNI scale Plant susceptibility to Phytophthora fragariae red stele root rot Plant susceptibility to Phytophthora fragariae red stele root rot SPF The plant susceptibility to red stele root rot caused by Phytophthora fragariae. 0-1 BOTRYITIS scale First runner date method Observe the plant and record the Julian date the first runners emerge at a specific location. resistance to Phytophthora cactorum crown rot Plant Plant resistance to Phytophthora cactorum crown rot The plant resistance to crown rot caused by Phytophthora cactorum. RPCCR FRpH pH The pH of fruit. Fruit pH Fruit Observe and rate average fruit shape on 3-5 fruit per genotype. Fruit shape RB method Fruit Fruit ripen days Number of days from flowering to ripe fruit. FRD development mg/L Cy-3-gluc Eq mg/L Pg-3-gluc kg−1 Observe and rate the average calyx size relative to fruit size on 3-5 fruit per genotype. Calyx size method Resistance to Neopestalotiopsis sp. scale 0-6 Observe and rate the resistance of the plant or plants per plot to Neopestalotiopsis sp., where 0 = complete resistance/symptomless and 6 = completely dead plants Fruit fresh weight 5L method Harvest and record the total weight of the 5 largest marketable fruit over the harvest season. 1-5 RMH scale Observe and rate the fruit skin color. Skin color 1-10 method 0-1 EVER scale 1-9 STAMENHGHT scale The plant susceptibility to leaf scorch caused by Diplocarpon earliana Plant susceptibility to Diplocarpon earliana SDE Plant susceptibility to leaf scorch caused by Diplocarpon earliana 1-5 SUSCEP scale Harvest date method Record the Julian date when first fruit ripens at a specific location. Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. Resistance to Phytophthora cactorum CR % method ECOGDDREQH fruit development Plant Growing degree days to first harvest. Plant fruit development Fruit fresh weight 1 method Harvest and record the average weight of the largest primary fruit harvested when 30-50% of the fruit on each plant are ripe. Fruit The weight of fresh fruit. fresh weight largest LFRW Largest fruit weight Chroma Index Plant susceptibility to Verticillium dahliae wilt The plant susceptibility to verticillium wilt caused by Verticillium dahliae. SVD Plant susceptibility to Verticillium dahliae wilt Plant resistance to Diplocarpon earlianum leaf scorch RDE The plant resistance to leaf scorch caused by Diplocarpon earliana. Plant resistance to Diplocarpon earlianum leaf scorch Leaf total antioxidant content Total antioxidant content of leaves. LFTOTANTIOX Leaf total antioxidant content scale_of Resistance to Colletotrichum acutatum anthracnose fruit rot % method Observe and rate the resistance of the fruit to anthracnose fruit rot caused by Colletotrichum acutatum. position Achene position ACHENEPOS The postion of achene relative to the fruit surface. Achene presence STAMENPRES Stamen The presence of stamens. Stamen presence Peduncle length method Observe average peduncle length and record it. Color uniformity method Observe fruit color uniformity from even or very slighly unevento strongly uneven. Resistance to Fusarium oxysporum f. sp. fragariae 1-5 method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Susceptibility to Colletotrichum gloeosporioides weekly method Observe plants weekly and record plant collapse due to C. gloeosporioides.Begin scoring for disease 1 week after inoculation and continue until the conclusion of the fruiting season. Measure disease severity by rating plant collapse using S to indicate susceptible plants and R to indicate resistant plants. 1-9 ACHENECOL scale Fruit development method Record the number of days from first flowering to ripe fruit. Fruit appearance method Observe fruit appearance from not uniform to completely uniform. Fruit The presence of powdery mildew on fruit. presence of powdery mildew PPMF Fruit presence of powdery mildew Plant Plant resistance to Meloidogyne hapla root knot nematode RMH The plant resistance to root knot nematode Meloidogyne hapla. resistance to Meloidogyne hapla root knot nematode Total anthocyanins Cy-3-gluc method Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5. R-S category Ratio 1-3 CALYXSZ scale Leaf pubescence image capturing method Collect fresh leaves into 50 mL tubes filled with water. Capture the leaf image using a stereo zoom microscope. Fruit uniformity of color uniformity of color Fruit Fruit uniformity of color. FRCOLORUNIF 1-7 PEDBRK scale 1-7 RUNNERNUM scale Achene ACHENEVIABLE Percentage of viable achenes The percentage of achenes that are viable. viable total anthocyanin content Fruit Total anthocyanins The total anthocyanins of fruit. TACY Observe and rate the resistance of the plant to leaf spot caused by Mycosphaerella fragariae. Resistance to Mycosphaerella fragariae method date Observe the number of fruit that a single plant sets in a specific location. Fruit set method Estimate the average skin toughness by dragging the thumb firmly over the skin on 3-5 fruit per genotype. Skin toughness method Observe average achene position relative to the fruit surface on 3-5 fruit per genotype and record it. Achene position 1-3 method Skin color chromameter reflect L* brightness method Measure color on two sides of 10 ripe undamaged and uniform fruit by using a chromameter, that includes three parameters: L* (Luminance) a* (red tone) and b* (yellow tone) where L* is a measure of brightness. Titratable acidity 0.5 g citric acid method A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid. Percentage of viable achenes method Observe and estimate the percentage of viable/filled achenes in 10% increments on 3-5 fruit per genotype. grams 1-5 RESIST scale Observe and rate the resistance of the plant to verticillium wilt caused by Verticillium dahliae, where 1 = highly resistant/symptomless and 9 = highly susceptible/severe symptoms Resistance to Verticillium dahliae scale 1-9 FLDATE100 date Whether or not the plant has an everbearing habit bearing type Everbearing EVERBERIN Plant Plant resistance to Mycosphaerella fragariae leaf spot RMYFR Plant The plant resistance to Mycosphaerella fragariae. resistance to Mycosphaerella fragariae leaf spot Resistance to Fusarium oxysporum f. sp. fragariae % method Observe and rate the resistance of the plant to fusarium wilt caused by Fusarium oxysporum f. sp. fragariae. Observe the truss location relative to the foliage canopy and record it. Truss exposure 1-5 method 1-9 FRSHAPE RB scale Runner Runner daughters The total number of daughter plantlets per plant harvested on a single day at a specific location. daughters DAU Observe and rate the resistance of the plant to charcoal rot caused by Microphomina phaseolina. Resistance to Macrophomina phaseolina method Luminance Fruit harvest date Harvest date HD Record the Julian date when first fruit ripens at a specific location. Taste the fruit and rate the average flavor of fruit as a perception of sweetness and presence of off-flavors on 3-5 fruit per genotype. Fruit flavor method Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush. Flower diameter UPOV method 1-9 FRFLCOL scale Skin gloss method Observe and rate average fruit skin gloss on 3-5 fruit per genotype. Measure FRAP using freshly prepared working FRAP solution (900 μl) and adding to 100 μl of standards or extracts. Standards curves using trolox (0–0.25 mM) and ferrous sulphate (0–0.5 mM) were run with each set of extracts and were linear within these ranges. Final result was expressed as trolox equivalents (μmoles/g FW). Ferric reducing antioxidant power trolox method 0-1 TREXPOS scale Measure titratable acidity in 10 ml of juice diluted with distilled water (1:2 v/v) and titrated with 0.1 N NaOH, to pH 8.2, and expressed in mEq of NaOH per 100 g of fruit. Titratable acidity NaOH method Resistance to Diplocarpon earlianum method Observe and rate the resistance of the plant to leaf scorch caused by Diplocarpon earlianum. Resistance to Meloidogyne hapla method Observe and rate the resistance of the plant to root knot nematode caused by Meliodogyne hapla. 0-1 CALPLUG scale average Observe and rate the average fruit skin color once to twice weekly for when fruit color is fully developed and 30-50% of the fruit on the plant are ripe. Skin color 1-9 method 1-9 CALYXREFLX scale Observe and rate the resistance of the plant to leaf blight caused by Phomopsis obscurans. Resistance to Phomopsis obscurans method Resistance to Phytophthora cactorum CR 1-8 method Observe and rate the resistance of the plant to crown rot caused by Phytophthora cactorum. 0-1 STAMPRES scale DNA/std DNA_IBL SKINCOL Skin Skin color Skin color on the sun side of a fully ripe fruit. color 1-9 SKINCOL scale Fruit length method Measure the length of the fruit from the calyx to the tip and record in mm. Observe the presence of absence of a central plug remaining in the fruit after manual calyx removal. Calyx plug method 1-9 FRUITSET scale Meq NaOH/100g 1-3 FRCOLUNI scale Susceptibility to Verticillium dahliae 1-5 method Observe and rate the susceptibility of the plant to verticillium wilt caused by Verticillium dahliae. method_of 1-9 FRYIELD scale 1-3 ACHENECOL UPOV scale 1-3 CALYXREFLX UPOV scale Number of flowers per truss estimation Observe the number of flowers per inflorescence. DNA/std DNA_ILF Calyx CALYXPLUG Presence or absence of central plug after calyx removal. Calyx plug plug 0-6 RESIST scale