FrP_Pmsr_mm
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FrM_FrMmsr_g
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
CLDM_LfDMmsr_mg
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
FrEqD_EqDmsr_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
InfN_InfNcnt_n
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
RaN_RaNStcmp_n/St
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PtAr_PtArmsr_ha
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
FrPA_PAmsr_mm2
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PlFlgDate_FlgDateest_date
Adana Alparslan Türkeş Science and Technology University - 013z3yn41,The Land Institute - 00jxaym78
Muhammet Sakirgolu - 0000-0002-7024-4348,Brandon Schlautman - 0000-0002-9983-259X
SdMoiC_SdMoiCmsr_%
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
LfSPAD_CLSPADmsr_idx
leaf SPAD
Adana Alparslan Türkeş Science and Technology University - 013z3yn41,The Land Institute - 00jxaym78
Muhammet Sakirgolu - 0000-0002-7024-4348
PlFlg_FlgPctcmp_%
Adana Alparslan Türkeş Science and Technology University - 013z3yn41,The Land Institute - 00jxaym78
Muhammet Sakirgolu - 0000-0002-7024-4348,Brandon Schlautman - 0000-0002-9983-259X
FoADLC_ADLmsr_g/kg
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
PlEDTF_DTFcmp_days
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
LfW_LfWmsr_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
RaN_RaNPlcmp_n/Pl
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdMaltC_SdSumsr_DMB
The Land Institute - 00jxaym78
Evan Craine - 0000-0002-6252-8973
FoYld_FoYldArcmp_kg/m2
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FrL_Lmsr_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
LfW_LfWprd_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
FrN_FrNRacmp_n/Ra
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PlDeath_PDest_01
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
FrN_FrNcnt_n
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
CLSLA_SLAcmp_mm2/mg
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
FoNDFC_NDFmsr_g/kg
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FrYld_FrYldPlcmp_g/Pl
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdYld_SdYldArcmp_kg/ha
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
FoSDM_FoSMmsr_kg
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
StN_StNcnt_n
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdZC_ZCmsr_DMB
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
FrYld_FrYldRacmp_g/Ra
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FrM_FrMmsr_kg
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
Sd1KK_SdMmsr_g
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdSucC_SdSumsr_DMB
The Land Institute - 00jxaym78
Evan Craine - 0000-0002-6252-8973
SdTSC_TSCmsr_DMB
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PcpM_PcpMmsr_mg
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
LfChlA_ChlAmsr_mg/g
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdPC_PCmsr_DMB
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PlMSC_MSCcmp_PGrS scale
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdEx_Exmsr_ratio
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
SdEqD_EqDmsr_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
PlDFDate_DFmsr_date
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdPA_PAmsr_mm2
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
SdCFC_CFCmsr_DMB
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
StdCnt_SCcmp_n/Pt
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
Resistant: No to minimal chlorosis of lower leaves
1
SdIC_ICmsr_DMB
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SLG_Gcmp_%
Seedlot germination percentage
Seedlot viability rate
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
Susceptible: Well developed symploms of chlorotic, necrotic and twisted terminal leaflets on at least one, but not all main stems
3
CLL_CLLmsr_mm
leaf length
Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Muhammet Sakirgolu - 0000-0002-7024-4348
FrW_Wmsr_mm
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdMoiC_SdMoiCprd_%
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
CanHt_Htmsr_cm
Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Muhammet Sakirgolu - 0000-0002-7024-4348
FrCol_Colmsr_HSV
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
PlVR_Vrest_VR1to5
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
FlCol_FlColmsr_CIELAB
Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Muhammet Sakirgolu - 0000-0002-7024-4348
RaL_RaLmsr_cm
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdP_Pmsr_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
FrCD_CDmsr_μm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
FlN_FlNcnt_n
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FlN_FlNRacmp_n/Ra
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdAC_ACmsr_DMB
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
RaN_RaNcnt_n
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdLactC_SdSumsr_DMB
The Land Institute - 00jxaym78
Evan Craine - 0000-0002-6252-8973
SdN_SdNcnt_n
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdTSuC_SdSumsr_DMB
The Land Institute - 00jxaym78
Evan Craine - 0000-0002-6252-8973
None (N)
1
FrAR_ARmsr_ratio
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
LfN_LfCLcmp_n/CL
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
PlODTF_DTFcmp_days
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
LfL_LfLprd_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
SdW_Wmsr_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
StN_StNPlcmp_n/Pl
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
StTh_StThmsr_mm
Adana Alparslan Türkeş Science and Technology University - 013z3yn41,The Land Institute - 00jxaym78
Ebrar Karabulut - 0000-0003-0030-2933
Late bud: > 2 nodes with buds; no flowers or seed pods
B2
PlGrS_PGrSest_PGrS scale
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PlRGDate_RGest_date
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FrYld_FrYldArcmp_kg/ha
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PlStEr_StErest_SE0-9
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FoCPC_CPCmsr_g/kg
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
FrYld_FrYldStcmp_g/St
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
SdDFC_DFCmsr_DMB
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdN_SdNRacmp_n/Ra
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
Moderate (M)
4
PlPctWS_WSest_%
Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Muhammet Sakirgolu - 0000-0002-7024-4348
FoFM_FoMmsr_kg
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FrYld_FrYldArcmp_g/m2
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FrSol_Solmsr_ratio
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdCD_CDmsr_μm
seed convexity defect length
seed hilum depth
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
FoDM_PctDMcmp_%
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PlDDTF_DTFcmp_days
Adana Alparslan Türkeş Science and Technology University - 013z3yn41,The Land Institute - 00jxaym78
Muhammet Sakirgolu - 0000-0002-7024-4348,Brandon Schlautman - 0000-0002-9983-259X
InfL_InfLmsr_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
StN_StNPlcmp_n/Pt
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
LfChl_ChlTotmsr_mg/g
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FoADFC_ADFmsr_g/kg
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
StL_StLmsr_cm
Adana Alparslan Türkeş Science and Technology University - 013z3yn41,The Land Institute - 00jxaym78
Muhammet Sakirgolu - 0000-0002-7024-4348,Brandon Schlautman - 0000-0002-9983-259X
SdL_Lmsr_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
LfChlB_ChlBmsr_mg/g
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PlN_PlNcnt_n
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PtAr_PtArmsr_m2
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdFrucC_SdSumsr_DMB
The Land Institute - 00jxaym78
Evan Craine - 0000-0002-6252-8973
FoDM_FoMmsr_kg
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
SdG_Gcnt_n
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
SdCC_CCcmp_DMB
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
Trace (T)
2
FoSFM_FoSMmsr_kg
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FrEx_Exmsr_ratio
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
SdM_SdMmsr_g
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdlEmDate_Emest_date
Adana Alparslan Türkeş Science and Technology University - 013z3yn41,The Land Institute - 00jxaym78
Muhammet Sakirgolu - 0000-0002-7024-4348,Brandon Schlautman - 0000-0002-9983-259X
SdAR_ARmsr_ratio
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
SdSDFC_DFCmsr_DMB
The Land Institute - 00jxaym78
Evan Craine - 0000-0002-6252-8973
FoYld_FoYldArcmp_kg/ha
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
LfA_PAmsr_mm2
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
LfL_LfLmsr_mm
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
CLA_PAmsr_mm2
The Land Institute - 00jxaym78
Bo Meyering - 0000-0002-1524-787X
Vegetative stage - no elongated stems
V1
SdPAC_PACmsr_DMB
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdYld_SdYldArcmp_g/m2
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
Resistant: Chlorosis of lower and middle leaves, but no chlorosis or necrosis of terminal leaves
2
SdYld_SdYldStcmp_g/St
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
FoAC_FoACmsr_%
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
SdSol_Solmsr_ratio
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
SdGlucC_SdSumsr_DMB
The Land Institute - 00jxaym78
Evan Craine - 0000-0002-6252-8973
Peak/Late Flowering: >
F2
SdIDFC_DFCmsr_DMB
The Land Institute - 00jxaym78
Evan Craine - 0000-0002-6252-8973
Seedling emergence
Em
SdYld_SdYldRacmp_g/Ra
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
SdYld_SdYldPlcmp_g/Pl
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
PlWKD_WKest_WKD1-5
Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Muhammet Sakirgolu - 0000-0002-7024-4348
FoMoiC_MoiCcmp_g/kg
The Land Institute - 00jxaym78
Brandon Schlautman - 0000-0002-9983-259X
Early bud: 1 to 2 nodes with buds; no flowers or seed pods
B1
SdCol_Colmsr_HSV
The Land Institute - 00jxaym78,Adana Alparslan Türkeş Science and Technology University - 013z3yn41
Brandon Schlautman - 0000-0002-9983-259X,Muhammet Sakirgolu - 0000-0002-7024-4348
Ripe seed pod: nodes with mostly brown mature seed pods
P3
FlFert_FlPctFertcmp_%
Adana Alparslan Türkeş Science and Technology University - 013z3yn41,The Land Institute - 00jxaym78
Muhammet Sakirgolu - 0000-0002-7024-4348,Brandon Schlautman - 0000-0002-9983-259X
Vegetative stage - stem elongation
E1
Slight (S)
3
Early flowering: one node with one open flower; no seed pods
F1
Late seed pod: > 3 nodes with green seed pods
P2
acronym
Susceptible: Plant dead
5
Very Severe (VS)
5
Early seed pod: 1 to 3 nodes with green seed pods
P1
Sainfoin ontology
Susceptible: Severe symptoms of chlorosis, necrosis, and twisting of all leaflets on all main stems
4
entity
attribute
Plant growth stage estimation
Description: Estimate the growth stage of a plant or set of plants
Materials:
Dependent_on:
Protocol:
1. For a single plant, match the current stage of plant growth to the descriptions in the plant growth stage categories.
2. For a group of plants, match the average growth stage of all the plants to the most representative category.
Leaflet area
The total area of a single leaflet or a the average area of a sample of leaflets.
LfA
Leaflet
area
Seed solidity
The ratio of a seeds contour area to its convex hull area.
SdSol
Seed
solidity
Seed germination
The total count of germinated seeds.
SdG
Seed
germination
Forage dry matter
The percentage of dry matter found in fresh forage
FoDM
Forage
dry matter
gram per 100 gram (dry matter basis)
Raceme length
The total length of a single raceme, or the mean length of several racemes
RaL
Raceme
length
Plant number counting
Description: Record the total number of plants in a group or sample
Materials:
Dependent_on:
Protocol:
1. Count all sampled plants, all the plants in one plot/sward, or all the plants in a row.
2. Express as an integer.
Inflorescence number counting
Description: Record the total number of inflorescences
Materials:
Dependent_on:
Protocol:
1. Collect inflorescences according to your sampling protocol
2. Count the total number of inflorescences.
3. Express as an integer.
Seedling emergence DTF computation
Description: Record the days to flowering (DTF) post seedling emergence
Materials:
Dependent_on: Seedling emergence date est [ISO 8601], Plant flowering date est [ISO 8601]
Protocol:
1. Record the number of days that have elapsed from the seedling emergence date to the flowering date.
2. Express DTF as an integer.
Seed sugar content measurement
Description: Determination of Simple Sugars in Cereal Products—HPLC Method
Materials:
Dependent_on:
Protocol:
1. See full protocol at AACC 80-04.01
Forage fresh mass
The total mass of fresh forage
FoFM
Forage
fresh mass
Flower percent fertilization computation
Description: Record the percentage of flowers that were fertilized on a raceme.
Materials: loupe (if needed)
Dependent_on: Legume fruit cnt [n], Flower number cnt [n]
Protocol:
1. Sample racemes according to your sampling protocol.
2. Record the flower number on each raceme
3. Record the fruit number on each raceme
4. For each raceme, divide the fruit number by the flower number to get a percentage of flowers fertilized.
5. For more than one raceme, record the average percent fertilization as a percentage
number per plot
milligram per gram
Seed sucrose content
The total sucrose content of a sample of seeds.
SdSucrC
Seed
sucrose content
Compound leaf area
The total area of a single compound leaf.
CLA
Compound leaf
area
Compound leaf SLA
The specific leaf area of a single compound leaf, or the average specific leaf area of a sample of compound leaves.
CLSLA
Compound leaf
SLA
Seed zinc content measurement
Description: Measure the zinc content in a sample of seeds
Materials:
Dependent_on:
Protocol:
1. See full protocol at AACC 40-70.01
Object equivalent diameter measurement
Description: Calculate the equivalent diameter of an image object
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. See https://docs.opencv.org/4.x/d1/d32/tutorial_py_contour_properties.html
Seed iron content
The iron content of a sample of seeds
SdIC
Seed
iron content
Plant mean stage count computation
Description: The average stage of development for a group of plants or plant canopy
Materials:
Dependent_on: Plant growth stage est {PGrS Scale]; Stem number cnt [n];
Protocol:
1. Sample stems according to your sampling protocol.
2. Tally the number of stems in that fall into each category.
3. Over all indices from the lowest growth stage index to the highest index, sum the product of the growth stage index and the number of stems in that growth stage divided by the number of stems.
percent
Leaflet width
The width of a leaflet, orthogonal to the main leaflet axis when measured at the widest point.
LfW
Leaflet
width
Seed yield per area computation
Description: Record the total seed yield per area
Materials: plot combine, laboratory scale
Dependent_on: Plot area msr [m2], Seed moisture content [%]
Protocol:
1. Drive plot combine through each plot and collect fruit or harvest plot by hand.
2. Remove pericarp from fruit and collect seeds in a paper bag.
3. Dry seeds to a specified % moisture content.
4. Get the dry weight of harvested seeds.
5. Divide the total dry weight of seeds by the plot area or harvested area.
6. Report yield at seed moisture content.
gram per raceme
Legume fruit mass measurement
Description: Record the total mass of a sample of legume fruits.
Materials: plot combine; laboratory seed thresher; column seed blower; digital scale;
Dependent_on:
Protocol:
1. Harvest legume fruits according to your sampling protocol.
2. Dry the harvested material.
3. Thresh the fruits off of the stems with a laboratory scale seed thresher (Wintersteiger LD 350).
4. Clean the fruit by using a column seed blower or running the fruit through the seed thresher a second time.
5. Record the total weight of the cleaned fruit.
Seed equivalent diameter
The diameter of a circle of equivalent area to the area of the seed
SdEqD
Seed
equivalent diameter
Ordinal DTF computation
Description: Record the ordinal days to flowering (DTF) from January 1st
Materials:
Dependent_on: January 1st [ISO 8601], Plant flowering date est [ISO 8601]
Protocol:
1. Record the integer number of days that have elapsed between the plant flowering date and January 1st on a given year.
Seed crude fat content measurement
Description: Determination of crude fat content in sample by Soxhlet or continuous extraction
Materials: extractor; filter paper; extraction thimbles 22x80mm; cotton (free from ether extract) or Pyrex glass wool; vacuum oven or vacuum desiccator for nonoxidative drying of sample; petroleum ether (redistilled, BP 30-60F)
Dependent_on:
Protocol:
1. See AACC 30-25.01
Seed projected area
The area of a seed as projected onto the 2-D surface of the image plane.
SdPA
Seed
projected area
Moisture content computation
Description: Record the moisture content of a forage sample
Materials:
Dependent_on: Forage dry mass [kg], Forage fresh mass [kg] | Forage subsample dry mass [g], Forage subsample fresh mass [kg]
Protocol:
1. Sample forage according to your sampling protocol.
2. Weigh both the fresh weight of the forage as well as the dried weight of the whole sample, or if a large quantity, just a subsample.
3. Find the difference between the fresh mass and the dry mass and divide by the fresh mass.
4. Multiply by 1000 if expressing as g/kg or leave as is to express as g/g or kg/kg.
ratio
Leaflet length
The length of leaflets measured from the proximal margin of the leaflet to the distal margin of the leaf.
LfL
Leaflet
length
Object extent measurement
Description: Calculate the object extent
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. Retrieve the total area of an object in an image
2. Create a bounding box around the image with cv.boundingRect()
3. Compute the extent as the ratio between the object area and the bounding box area.
4. See https://docs.opencv.org/4.x/d1/d32/tutorial_py_contour_properties.html
Seed number
The total number of seeds counted, estimated, or observed
SdN
Seed
number
Flower fertilization
The percentage of flowers on a raceme or group of racemes that were fertilized and developed into legume fruit.
FlFert
Flower
fertilization
Seed crude fat content
The crude fat content of a sample of seeds
SdCFC
Seed
crude fat content
Legume fruit length
The length of a legume fruit along its main axis.
FrL
Legume fruit
length
Legume fruit number per raceme computation
Description: Record the total number of legume fruits per raceme.
Materials: mechanical or digital hand-held counter;
Dependent_on: Legume fruit number cnt [n]; Raceme number cnt [n];
Protocol:
1. Sample racemes according to your sampling protocol.
2. Place the racemes into a pollination bag.
3. In the lab, strip the fruit from each raceme.
4. Count and record the total number of fruit.
5. Divide the total number of fruit by the number of racemes sampled
Stem thickness
The thickness of the stem measured at the midpoint between the second and third nodes on a mature stem.
StTh
Stem
thickness
square millimeter per milligram
kilogram per square meter
Forage subsample mass measurement
Description: Record the mass of a forage subsample.
Materials: digital scale;
Dependent_on:
Protocol:
1. Select a subsample of forage from a larger pool of forage
2. For fresh forage mass measurement, place the subsample on the scale and record the weight at the time of or shortly after harvesting.
3. For dry forage mass measurement, dry the subsample in a drying oven until constant weight, place the material on a scale, and record the weight.
day
Stem length measurement
Description: Measure the length of one representative stem by stretching it out end to end.
Materials: ruler or tape measure;
Dependent_on:
Protocol:
1. Select one representative stem from a single plant.
2. Harvest the stem by cutting just above the soil level near the crown.
3. Stretch the stem out on a flat surface.4. Measure the stem from the proximal end to the distal end at the growth apex.
Legume fruit convexity defect
The average length of the three largest convexity defects on the dried legume fruit crest
FrCD
Legume fruit
convexity defect
Raceme length measurement
Description: Measure the length of a raceme
Materials:
Dependent_on:
Protocol:
1. Select one inflorescence, starting at the proximal end, search for the most proximal flower or flower scar and use this as a starting point.
2. Measure the distance from the starting point to the distal end of the inflorescence.
3. Record this length as the raceme length.
4. Repeat for as many racemes as necessary and take the average raceme length.
Stem number
The total number of stems counted, estimated, or observed
StN
Stem
number
Object projected area measurement
Description: Calculate the projected area of an object
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. Find an object contour using cv.findContours()
2. Export the contour area as cv.contourArea()
3. See https://docs.opencv.org/4.x/d1/d32/tutorial_py_contour_properties.html
Plant growth stage
The current growth stage of a plant or the average growth stage of a group of plants or plot
PlGrS
Plant
growth stage
Plot area measurement
Description: Measure the total area of a plot
Materials: tape measure
Dependent_on:
Protocol:
1. Find the corner of the plot.
2. Record the actual length and width of the plot.
3. Multiply and express in square units of length or in some standard area unit.
Neutral detergent fiber measurement
Description: Determination of forage fiber content by the neutral detergent method.
Materials:
Dependent_on:
Protocol:
1. See https://www.journalofdairyscience.org/article/S0022-0302(91)78551-2/pdf
Stem thickness measurement
Description: Measurement of stem thickness using digital calipers
Materials: digital calipers;
Dependent_on:
Protocol:
1. Select a mature sainfoin stem
2. Locate the midpoint between the second and third nodes on the stem and record the thickness using calipers to the nearest tenth of a millimeter.
Note: Can be used as a single stem trait or take the average if sampling several stems on a plant/plot.
Plant ordinal DTF
The time it takes in days for a plant or group of plants to flower starting from January 1st of a given year.
PlODTF
Plant
ordinal DTF
Seed iron content measurement
Description: Measure the iron content in a sample of seeds
Materials:
Dependent_on:
Protocol:
1. See full protocol at AACC 40-70.01
gram per kilogram
square millimeter
Forage subsample fresh mass
The total fresh mass recorded on a subsample of the total biomass
FoSFM
Forage
subsample fresh mass
Legume fruit perimeter
The perimeter of the 2-D projected contour of a legume fruit.
FrP
Legume fruit
perimeter
Legume fruit yield
The total mass of the sampled fruit divided by an amount of some other entity
FrYld
Legume fruit
yield
index
Seed 1000K weight
The total mass of 1000 seeds at XX% moisture content
Sd1KK
Seed
1000K weight
Plant death estimation
Description: Estimating whether a single plant is dead or not, by scoring with a binary outcome.
Materials:
Dependent on:
Protocol: 1. Find a single plant, and determine if the plant is dead or not.
2. If dead, score as a 1, if alive, score as a 0.
Note: This data is only applicable to a single plant
Object perimeter measurement
Description: Calculate the perimeter of an object in an image
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. Find object contour using cv.findContours()
2. Record perimeter of contours as cv.arcLength(cont)
3. See https://docs.opencv.org/4.x/d1/d32/tutorial_py_contour_properties.html
Seed glucose content
The total glucose content of a sample of seeds.
SdGlucC
Seed
glucose content
Forage crude protein content
The total crude protein content of forage
FoCPC
Forage
crude protein content
Verticillium resistance estimation
Description: A simple method to rate plants according to their resistance to verticillium infections
Materials:
Dependent_on:
Protocol: 1. See full protocol at https://www.naaic.org/stdtests/verticil.pdf
Forage moisture content
The total moisture content calculated in fresh forage.
FoMoiC
Forage
moisture content
Seed mass measurement
Description: Record the total mass of a sample of seeds.
Materials: cleaned fruit; laboratory seed cleaner/dehuller; digital scale;
Dependent_on: Seed moisture content [%]
Protocol:
1. Sample fruit according to your sampling protocol
2. Place cleaned fruit into the seed cleaning/dehulling machine (Haldrup LT15).
3. Process the fruits until the majority of the pericarps have been removed.
4. Place the cleaned seed onto a laboratory scale and record the mass in the appropriate units.
5. Report seed mass at seed moisture content.
Legume fruit equivalent diameter
The diameter of a circle of equivalent area to the area of the legume fruit
FrEqD
Legume fruit
equivalent diameter
Legume fruit yield per plant computation
Description: Compute the total yield of legume fruit per plant
Materials: clippers, bundle thresher, sieves/blower columns, laboratory balance
Dependent_on: Legume fruit mass msr [g], Plant number cnt [n]
Protocol:
1. Divide the total mass of a sample of legume fruit by the number of plants they were sampled from.
2. Express yield as g/plant
Canopy height
The height of a plant, plot, or group of plants when measured from the soil surface to the growing apex or average growing apex of the plant(s).
CanHt
Canopy
height
Legume fruit number counting
Description: Record the total number of legume fruit observed.
Materials: mechanical or digital hand held counter;
Dependent_on:
Protocol:
1. Sample legume fruits according to your sampling protocol.
2. Count and record the total number of legume fruit in a sample regardless of the presence/absence of a seed inside the fruit.
Stand count
The number of plants in a stand or group.
StdCnt
Stand
count
Forage NDF Content
The total forage fiber content as determined by the neutral detergent fiber method.
FoNDFC
Forage
NDF Content
Plant mean stage count
The weighted average growth stage of a sample of stems
PlMSC
Plant
mean stage count
Legume fruit number
The total number of fruit either as a standalone count or expressed relative to another body
FrN
Legume fruit
number
milligram
Seed dietary fiber content measurement
Description: Measure the total dietary fiber in a sample of seeds
Materials:
Dependent_on:
Protocol:
1. See AACC 32-07.01 (method1)
2. See AOAC 991.43 (method2)
micrometer
Stem length
The total length of a single plant stem or the mean value of several stems
StL
Stem
length
Compound leaf length
The total length of a compound leaf from the base of the petiole to the distal end of the terminal leaflet.
CLL
Compound leaf
length
Plant verticillium resistance
The resistance of sainfoin germplasm to Verticillium wilt
PlVR
Plant
verticillium resistance
Stand count computation
Description: A count of the total living plants in a plot or group of plants
Materials: Handheld clicker and datasheet, or mobile phenotyping app.
Dependent_on:
Protocol: 1. Go through the plot and count the total number of living plants in the plot.
Forage ash content measurement
Description: Determine the ash content of a sample of forage
Materials: muffle furnace; porcelain crucibles; laboratory balance;
Dependent_on:
Protocol:
1. Sample forage according to your sampling protocol.
2. Weigh crucible and record weight to 4 decimal places.
3. Weigh approximately 2 g into crucible.
4. Record weight to 4 decimal places.
5. Ash sample at 600oC for 2 h. (Bring temperature rapidly to 600)
6. Cool in desiccator and weigh within 1 h after reaching room temperature.
7. Weigh ashed sample and record weight to 4 decimal places.
8. Calculate % Ash and record value with one decimal.
9. https://www.clemson.edu/public/regulatory/ag-srvc-lab/feed-forage/procedure20.html
Legume fruit yield per stem per computation
Description: Compute the total yield of legume fruit per stem
Materials: laboratory balance Dependent_on: Legume fruit mass msr [g], Stem number cnt [n]
Dependent on:
Protocol: 1. Sample stems according to your sampling protocol.
2. Sample stems according to your sampling protocol.
3. Weigh the fruit in g.
4. Divide the fruit mass by the number of stems to express fruit yield in g/stem.
Seed phytic acid content
The phytic acid content of a sample of seeds
SdPAC
Seed
phytic acid content
Forage ADF content
The total forage fiber content as determined by the acid detergent fiber method.
FoADFC
Forage
ADF content
Seed moisture content prediction
Description: Predict the moisture content of a sample of seeds from NIR spectra
Materials: handheld NIR spectrometer
Dependent_on:
Protocol:
1. Sample seeds according to your sampling protocol.
2. Take NIR measurements with a handheld NIR spectrometer.
3. Predict the moisture content from your NIR standard curves.
number per plant
Legume fruit yield per area computation
Description: Compute the total yield of sainfoin fruits per unit area
Materials:
Dependent_on: Legume fruit mass msr [g], Plot area msr [m2]
Protocol:
1. After harvesting a legume fruit sample and recording the mass, record the total plot area that the legume fruits were harvested from.
2. Divide the fruit mass by the area, and express yield in g/m2
Forage subsample dry mass
The total dry mass recorded on a subsample of the entire biomass
FoSDM
Forage
subsample dry mass
Seed total sugar content
The total sugar content of a sample of seeds. Is the sum total of the glucose, fructose, sucrose, maltose, lactose, and other minor sugars in the sample.
SdTSuC
Seed
total sugar content
Seed color
The average color of a seed or group of seeds from a plant, expressed as a vector of float values in the color model of choice such as RGB or HSV.
SdCol
Seed
color
Plant flowering
The percentage of plants in a plot or group of plants that are flowering.
PlFlg
Plant
flowering
Leaf dry mass measurement
Description: Determine the dry mass of a compound leaf, leaflet, or a mixed sample of leaves
Materials: sample bags, analytical balance
Dependent_on:
Protocol: 1. Place a sample of leaves in a sample bag.
2. Dry until the bag has reached a constant weight.
3. Record the dry mass of the sample in the appropriate units.
variable_of
Forage yield per area computation
Description: Calculate the total mass of forage harvested per unit area.
Materials: tape measure; scale;
Dependent_on: Forage fresh mass msr [kg] | Forage dry mass msr [kg]; Plot area msr [m2];
Protocol:
1. Record the weight for forage fresh mass or forage dry mass.
2. Divide the mass by the total area of the harvested plot.
Raceme number counting
Description: Count the total number of racemes
Materials:
Dependent_on:
Protocol:
1. Sample racemes according to your sampling protocol
2. Count all racemes and express as an integer
Flower number counting
Description: Count the total number of flowers present
Materials: loupe (if needed);
Dependent_on:
Protocol:
1. Sample racemes, inflorescences, stems, or plants according to your sampling protocol.
2. Count and record the total number of visible flowers and flower scars that are present.
3. Express as an integer
Seed lactose content
The total lactose content of a sample of seeds.
SdLactC
Seed
lactose content
Seed moisture content
The total moisture content of a sample of seeds
SdMoiC
Seed
moisture content
Seed carbohydrate content
The carbohydrate content of a sample of seeds
SdCC
Seed
carbohydrate content
Object length measurement
Description: Calculate an objects length
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. Fit either an ellipse or a rotated bounding box(cv.minAreaRect()) to an object.
2. Export the object length as either the length of the major axis (ellipse) or the longer side of the rotated bounding box.
3. See https://docs.opencv.org/4.x/d1/d32/tutorial_py_contour_properties.html
Compound leaf length measurement
Description: Measurement of the length of a compound leaf
Materials: Metric ruler or tape measure
Dependent_on:
Protocol:
1. Select a mature, fully-expanded compound leaf
2. Lay the compound leaf out on a flat surface and record the length from the proximal end of the petiole to the distal end of the terminal leaflet.
Plant flowering date
The flowering date of a plant or a group of plants.
PlFlgDate
Plant
flowering date
Leaf total chlorophyll content measurement
Description: Determine the concentration of total chlorophyll in a leaf sample
Materials: See protocol
Dependent_on:
Protocol:
1. See protocol in http://www.chem.ucla.edu/dept/Faculty/merchant/pdf/porra_et_al_1989.pdf
Seed perimeter
The perimeter of the 2-D projected contour of a seed.
SdP
Seed
perimeter
VR1to5
date
Plant defoliation DTF
The time it takes in days for a plant or group of plants to flower starting from a defoliation event.
PlDDTF
Plant
defoliation DTF
Plant winter survival
The amount of plants that survived the winter and regrow in the spring
PlPctWS
Plant
winter survival
square meter
Seed number per raceme computation
Description: Record the total number of seeds per raceme
Materials:
Dependent_on: Seed number cnt [n], Raceme number cnt [n]
Protocol:
1. Select racemes according to your sampling protocol.
2. Strip the fruit from each raceme and remove the seeds from each fruit.
3. Count and record the number of seeds in each raceme.
4. Divide the total number of seeds by the number of racemes sampled
Pericarp mass
The total mass of the legume fruit pericarp.
PcpM
Pericarp
mass
WKD1-5
Leaflet width measurement
Description: Record the average leaflet width
Materials: metric ruler or tape measure
Dependent_on:
Protocol:
1. Select a compound leaf according to you sampling protocol
2. Measure the length of 5 leaflets on the compound leaf - 2 from either side of the compound leaf and the terminal leaflet.
3. Starting at the widest portion of the leaflet, measure from one leaflet margin to the margin on the opposite edge, orthogonal to the central axis.
4. Record the average width of all 5 leaflets.
Raceme number per stem computation
Description: Compute the number of racemes per stem
Materials:
Dependent_on: Raceme number cnt [n], Stem number cnt [n]
Protocol:
1. For one plant, sample stems according to your sampling protocol.
2. Count the number of racemes on each stem.
3. Divide the number of racemes by the number of stems sampled.
Seed convexity defect
The length of the largest convexity defect of a seeds projected area. Generally located at the seed hilum.
SdCD
Seed
convexity defect
Spring regrowth date estimation
Description: Estimate the date of the appearance of spring regrowth of established plants in a plot
Materials:
Dependent_on:
Protocol:
1. For a single plant, plot, or group of established plants, record the date when 50% of the plants have new spring growth.
Object solidity measurement
Description: Calculate an objects solidity
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. Find object contour using cv.findContours()
2. Get object convex hull using cv.convexHull()
3. Get the object area using cv.contourArea()
4. Express solidity as the ratio between the object area and the convex hull area.
5. See https://docs.opencv.org/4.x/d1/d32/tutorial_py_contour_properties.html
Stem number per plant computation
Description: Record the total number of stems per plant
Materials:
Dependent_on: Stem number cnt [n], Plant number cnt [n]
Protocol:
1. Sample plants according to your sampling protocol.
2. For each plant, separate and count the stems one by one.
3. Total the number of stems for all plants and divide by the number of plants.
Inflorescence number
The total number of inflorescences observed or sampled
InfN
Inflorescence
number
Winter kill estimation
Description: Estimate the winter kill damage of a plant or plot
Materials:
Dependent_on:
Protocol:
1. Observe winter kill of a single plant or the average damage of plants in a plot and rate it on the winter kill damage scale
Leaflet length prediction
Description: Measure the average leaflet length
Materials: Image analysis software, OpenCV scripts, etc.
Dependent_on:
Protocol:
1. Select a compound leaf according to your sampling protocol.
2. Select 5 leaflets on each compound leaf - 2 from either side of the compound leaf and the terminal leaflet.
3. Lay all 5 leaflets out on a flatbed scanner or photography light table with either a known scale or a scale included in the scene and acquire an image.
4. Use image processing to extract the length of the rotated bounding box of each leaflet.
5. Convert from pixels to mm using the known scale.
6. Report the average length in mm for all 5 leaflets.
kilogram
Seed mass
The total mass of seeds observed or sampled.
SdM
Seed
mass
Seed yield per stem computation
Description: Record the seed yield per stem
Materials:
Dependent_on: Seed mass msr [g], Stem number cnt [n], Seed moisture content [%]
Protocol:
1. Sample stems on a plant according to your sampling protocol.
2. For each stem, strip all fruit from all raceme into a pan.
3. Remove the pericarps from each fruit and collect the seeds.
4. Dry seeds to specified % moisture.
5. Weigh the seeds.
6. Divide the total weight by the number of stems.
7. Report yield at seed moisture content.
millimeter
PGrS
Defoliation DTF computation
Description: Record the days to flowering (DTF) post defoliation
Materials:
Dependent_on: Plant defoliation event date msr [ISO 8601], Plant flowering date est [ISO 8601]
Protocol:
1. Record the number of days that have elapsed from the defoliation event date to the flowering date.
2. Express DTF as an integer.
Seed yield per plant computation
Description: Record the seed yield per plant
Materials:
Dependent_on: Seed mass msr [g], Plant number cnt [n], Seed moisture content [%]
Protocol:
1. Sample plants according to sampling protocol.
2. Place whole plant through a large bundle thresher (Almaco, Vogel,etc.) or even strip all fruit off by hand.
3. Remove the seeds from the pericarp.
4. Clean seeds using sieves and/or blower columns and/or indent cylinders to remove all foreign material.
5. Finally, weigh the seeds and divide by the number of plants threshed.
6. Report at seed moisture content.
Object width measurement
Description: Calculate the width of an object
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. Fit a rotated bounding box or an ellipse to an image object
2. Record the object width as the minor axis length of the ellipse or the length of the smaller side of a rotated bounding box
Forage dry mass
The total mass of dried forage
FoDM
Forage
dry mass
HSV
Object color measurement
Description: Calculate the average color of an object
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. After segmenting the image using basic image processing algorithms, select objects using cv.findContours() etc.
2. Find the average of all pixels in the contour
3. Express as a vector of HSV values.
number
Seed extent
The ratio of the seed contour area to the bounding box area.
SdEx
Seed
extent
Plant winter kill damage
The winter damage observed on a plant or group of plants
PlWKD
Plant
winter kill damage
Leaflet number
The total number of leaflets counted
LfN
Leaflet
number
Plant regrowth date
The ISO 8601 date that established plants have new spring regrowth after a dormant period
PlRGDate
Plant
regrowth date
Seed dietary fiber content
The dietary fiber content of a sample of seeds.
SdDFC
Seed
dietary fiber content
Seed protein content measurement
Description: Determination of crude protein content by pyrolysis and combustion.
Materials:
Dependent_on:
Protocol:
1. See AACC 46-30.01
Object aspect ratio measurement
Description: Calculate the ratio of the objects bounding box width to its height
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. Create a bounding box around a segmented object using cv.boundingrect(obj).
2. Extract the bounding box width and height.
3. Express the aspect ratio as the width/height.
4. See https://docs.opencv.org/4.x/d1/d32/tutorial_py_contour_properties.html
Legume fruit width
The width of a legume fruit along its minor axis orthogonal to the main axis along the image plane
FrW
Legume fruit
width
Seed ash content
The ash content of a sample of seeds
SdAC
Seed
ash content
Flower color measurement
Description: Measure the color of flowers in CIELAB color space
Materials: handheld or benchtop colorimeter
Dependent_on:
Protocol:
1. Sample flowers according to your sampling protocol.
2. Measure the color of a flower or a set of flowers using the colorimeter.
3. Express the average value as a vector [x, y, z] in the CIELAB color model.
SE0-9
Legume fruit extent
The ratio of the legume fruit contour area to the bounding box area.
FrEx
Legume fruit
extent
Seed soluble dietary fiber content
The dietary fiber content in the soluble fraction of a sample of seeds.
SdSDFC
Seed
soluble dietary fiber content
Inflorescence length measurement
Description: Record the average inflorescence length
Materials: metric ruler or tape measure
Dependent_on: Inflorescence number cnt [n]
Protocol:
1. Sample inflorescences according to your sampling protocol
2. Measure each inflorescence by laying it out on a flat surface and recording the length in mm from the proximal end of the peduncle to the distal end of raceme.
3. If measuring one inflorescence, report the length. If measuring multiple, report the average length of all inflorescences measured.
Winter survival estimation
Description: Estimate the winter survival of a plot of plants
Materials:
Dependent_on: Plant number cnt [n]
Protocol:
1. For each plot or group of plants, count the number of plants that have regrowth in the spring.
2. Divide the number of plants with regrowth by the total number of plants in the plot
3. Express as a percentage.
Leaflet total chlorophyll content
The total chlorophyll content in a leaflet
LfChl
Leaflet
total chlorophyll content
Seed ash content measurement
Description: Measure the total ash content of seed flour by incinerating in a muffle furnace
Materials: muffle furnace with pyrometer and temperature control; broad shallow ashing dish; scale;
Dependent_on:
Protocol:
1. Weigh 3-5 +/- 0.0001 g of well mixed sample into ashing dish that has been ignited, cooled in desiccator, and weighed soon after attaining room temperature.
2. Place in muffle furnace at 550F for soft wheat flours or 575-590 for hard wheat flours. Incinerate until light gray ash is obtained or to constant weight. Ash must not be allowed to fuse. Cool in desiccator and weigh soon after room temperature is attained.
3. See AACC 08-01.01
Seedling emergence date
The emergence date of new shoots in the spring
SdlEmDate
Seedling
emergence date
Leaflet width prediction
Description: Measure the average leaflet width
Materials: Image analysis software, OpenCV scripts, etc.
Dependent_on:
Protocol:
1. Select a compound leaf according to your sampling protocol.
2. Select 5 leaflets on each compound leaf - 2 from either side of the compound leaf and the terminal leaflet.
3. Lay all 5 leaflets out on a flatbed scanner or photography light table with either a known scale or a scale included in the scene and acquire an image.
4. Use image processing to extract the width of the rotated bounding box of each leaflet.
5. Convert from pixels to mm using the known scale.
6. Report the average width in mm for all 5 leaflets.
Pericarp mass measurement
Description: Measure the mass of fruit pericarps
Materials: laboratory balance;
Dependent_on:
Protocol:
1. For one plant or one stem, remove all of the fruit.
2. For each fruit, remove the pericarp from the fruit.
3. Place the pericarps together and weigh.
Stem number counting
Description: Record the number of stems on one plant
Materials:
Dependent_on:
Protocol:
1. For a single plant, count the number of stems and express as an integer.
Plant emergence DTF
The time it takes in days for a plant or group of plants to flower starting from the seedling emergence date
PlEDTF
Plant
emergence DTF
Plot area
The total area of an experimental plot
PtAr
Plot
area
Legume fruit solidity
The ratio of a legume fruits contour area to its convex hull area.
FrSol
Legume fruit
solidity
Seed maltose content
The total maltose content of a sample of seeds.
SdMaltC
Seed
maltose content
number per stem
Plant defoliation event date
The date at which a plant or plot of established plants was defoliated either by swathing or seed harvesting
PlDFDate
Plant
defoliation event date
Forage yield
The total mass of forage harvested or sampled relative to some other value
FoYld
Forage
yield
Acid detergent fiber measurement
Description: Measurement of acid detergent fiber with acid detergent solution
Materials: See protocol
Dependent_on:
Protocol:
1. See https://uwlab.soils.wisc.edu/wp-content/uploads/sites/17/2015/09/forage_ADF.pdf
Compound leaf dry mass
The dry mass of a single compound leaf or a sample of compound leaves.
CLDM
Compound leaf
dry mass
Seed width
The width of a seed along its minor axis orthogonal to the main axis along the image plane.
SdW
Seed
width
Seed protein content
The protein content of a sample of seeds
SdPC
Seed
protein content
Legume fruit aspect ratio
The ratio of the legume fruit bounding box width to its height.
FrAR
Legume fruit
aspect ratio
Seed carbohydrate content computation
Description: A quick, indirect computation of total seed carbohydrates based.
Materials:
Dependent_on: Seed ash content msr [DMB], Seed moisture content msr [%], Seed crude fat content msr [DMB], Seed protein content msr [DMB]
Protocol:
1. Calculate the seed carbohydrate content according to the formula
Leaflet chlorophyll b content
The total chlorophyll B content in a leaflet
LfChlB
Leaflet
chlorophyll b content
Specific leaf area computation
Description: The specific leaf area of any leaf parts, compound leaf/leaves, or leaflet(s).
Materials: Scanner or camera, internal standard, paper bags.
Dependent_on: leaf/leaflet dry mass, leaf/leaflet area.
Protocol: 1. Scan/image the leaf parts and calculate the total area in mm2.
2. Dry the leaves until the dry mass is constant. Record in mg
3. Divide the area by the mass.
Object convexity defect measurement
Description: Calculate the average length of a convexity defect in an image
Materials: seed image, python-opencv
Dependent_on:
Protocol:
1. See examples at https://theailearner.com/2020/11/09/convexity-defects-opencv/
Seed length
The length of a seed along its main axis.
SdL
Seed
length
Seed yield
The total yield of the seeds of the plant. This is express in an amount of unit relative to another amount, i.e. land area, number of plants, plant weight, etc
SdYld
Seed
yield
Germination rate computation
Description: Measure the germination rate of a seedlot
Materials:
Dependent_on: Seed number cnt [n], Seed germination cnt [n]
Protocol: 1. Divide the Seed germination cnt [n] of a seedlot sample by the total Seed number cnt [n] in the sample.
CIELAB
binary outcome
Forage mass measurement
Description: Record the mass of forage material
Materials: digital scale;
Dependent_on:
Protocol:
1. Harvest forage material either by hand or with a forage harvester.
2. For fresh forage mass measurement, place the forage sample on the scale at the time of or shortly after harvesting and record the weight.
3. For dry forage mass measurement, dry the forage to constant weight in a drying oven, place the material on a scale, and record the weight.
kilogram per hectare
Leaflet SPAD
The SPAD value for a leaflet or group of leaflets.
LfSPAD
Leaflet
SPAD
number per compound leaf
Legume fruit mass
The total mass of the sampled fruit
FrM
Legume fruit
mass
Germination counting
Description: Count the number of germinated seeds in a group
Materials: Warm water, beaker, germination paper, enclosed container.
Dependent_on:
Protocol: 1. Soak a subset of seeds (minimum of 10, ideally around 100) in warm water (120F) for 30 minutes.
2. Place on moist germination paper.
3. Cover seeds and place at room temperature for 5-7 days.
4. Count the number of seeds that have germinated
Note: The enclosed container can be anything that holds the germination paper and keeps the paper moist.
Seed phytic acid content measurement
Description: Measure the amount of phytic acid in a seed sample
Materials:
Dependent_on:
Protocol:
1. See full protocol at AACC 40-70.01
Forage ADL content
The total forage lignin content as determined by one of several methods
FoADLC
Forage
ADL content
Leaflet SPAD measurement
Description: Measurement of the average SPAD value of leaflets on a compound leaf
Materials: SPAD meter
Dependent_on:
Protocol:
1. Select a compound leaf according to your sampling protocol.
2. Take SPAD readings on a total of 5 leaflets on the compound leaf - 2 from either side of the compound leaf and the terminal leaflet.
3. Record the average SPAD value of all 5 leaflets as the SPAD value for the compound leaf.
4. See notes in https://pubmed.ncbi.nlm.nih.gov/17342446/
Seed aspect ratio
The ratio of the seed bounding box width to its height.
SdAR
Seed
aspect ratio
Plant stem erectness
The proportion of plants within a sampled plot/sward that are resistant to stem lodging, i.e. have stem angles > 45 degrees.
PlStEr
Plant
stem erectness
Flower color
The color of a flower or group of flowers from a plant, expressed as a vector of float values on a color scale of choice.
FlCol
Flower
color
Leaf chlorophyll B content measurement
Description: Determine the concentration of chlorophyll B in a leaf sample
Materials: See protocol
Dependent_on:
Protocol:
1. See protocol in http://www.chem.ucla.edu/dept/Faculty/merchant/pdf/porra_et_al_1989.pdf
Seed fructose content
The total fructose content of a sample of seeds.
SdFrucC
Seed
fructose content
Flowering date estimation
Description: Record the date at which 50% of all plants have at least one flower.
Materials:
Dependent_on:
Protocol:
1. For each plant, plot, or group of plants, record the date at which 50% of all plants have at least one flower.
Plant death
Whether or not a single plant lives or dies.
PlDeath
Plant
death
Raceme number
The total number of racemes counted, estimated, or observed
RaN
Raceme
number
gram
Seed number counting
Description: Record the total number of seeds
Materials:
Dependent_on:
Protocol:
1. In a sample, record the total number of seeds.
2. Express as an integer.
Leaflet chlorophyll a content
The total chlorophyll A content in a leaflet
LfChlA
Leaflet
chlorophyll a content
Seed yield per raceme computation
Description: Record the seed yield per raceme
Materials:
Dependent_on: Seed mass msr [g], Raceme number cnt [n], Seed moisture content [%]
Protocol:
1. Sample racemes on a plant according to your sampling protocol.
2. For each raceme, strip all fruit into a pan.
3. Remove the pericarps from each fruit and collect the seeds.
4. Dry seeds to specified % moisture.
5. Weigh the seeds.
6. Divide the total weight by the number of racemes.
7. Report yield at seed moisture content.
Plant natural height measurement
Description: Measure the natural height of plants
Materials: metric ruler/tape measure
Dependent_on:
Protocol:
1. For a single plant, place the origin of the ruler at the base of the plant on the surface of the soil.
2. Record the vertical distance to the growing apices of the plant or the average height of the plant if the growing apices are lower than the tallest points of the plant (i.e. when the growth habit is not erect).
3. Repeat for as many plants as necessary and average the results for the entire plot, group.
Seed total starch content
The total starch content of a sample of seeds
SdTSC
Seed
total starch content
Seed zinc content
The zinc content of a sample of seeds
SdZC
Seed
zinc content
Crude protein content measurement
Description: Determination of crude protein content by macro Khedjal method
Materials: See protocol
Dependent_on:
Protocol:
1. See https://uwlab.soils.wisc.edu/wp-content/uploads/sites/17/2015/09/forage_CP.pdf
Leaf chlorophyll A content measurement
Description: Determine the concentration of chlorophyll A in a leaf sample
Materials: See protocol
Dependent_on:
Protocol:
1. See protocol in http://www.chem.ucla.edu/dept/Faculty/merchant/pdf/porra_et_al_1989.pdf
Leaflet length measurement
Description: Record the average leaflet length
Materials: metric ruler or tape measure
Dependent_on:
Protocol:
1. Select a compound leaf according to your sampling protocol.
2. Measure the length of 5 leaflets on each compound leaf - 2 from either side of the compound leaf and the terminal leaflet.
3. Start at the proximal base of the leaflet margin and measure until the distal margin of the leaf along the central axis.
4. Record the average length of all 5 leaflets.
Legume fruit yield per raceme computation
Description: Compute the total yield of legume fruit per raceme
Materials: laboratory balance
Dependent_on: Legume fruit mass msr [g], Raceme number cnt [n]
Protocol:
1. Sample racemes according to your sampling protocol.
2. Strip the fruit of the raceme into a pan.
3. Weigh the fruit.
4. Divide the fruit mass by the number of racemes to express fruit yield in g/raceme.
Seed moisture content measurement
Description: Determination of moisture content as measured by sample weight loss in an oven under specified conditions.
Materials: laboratory mill with 18-20 mesh screen; oven capable of maintaining 130F +/- 1F; aluminum moisture dishes 55mm dia and 15mm height; airtight desiccator with activated alumina; analytical balance with mg resolution;
Dependent_on:
Protocol:
1. Grind 30-40g of sample in the mill.
2. Mix rapidly with a spoon or spatula and immediately transfer a 2-3g portion to each of two or more tared moisture dishes.
3. Cover and weigh dishes at once.
4. Subtract the tare weights and record weight of sample.
5. Clean mill in between samples.
6. Uncover dishes and place them on shelf of oven. Place covers under the dished.
7. Insert shelf in oven at level of thermometer bulb.
8. Heat for exactly 60 min after the oven recovers its temperature.
9. Remove the shelf and dishes from the over, cover rapidly (using rubber finger insulators) and transfer to desiccator as quickly as possible.
10. Weigh dishes after they reach room temperature (45-60 min usually).
11. Determine loss in weight as moisture.
12. Replicate determinations must be within 0.2% moisture.
13. See AACC 44-15.02
Legume fruit projected area
The area of a legume fruit as projected onto the 2-D surface of the image plane.
FrPA
Legume fruit
projected area
Plant number
The total number of plants observed.
PlN
Plant
number
gram per square meter
Inflorescence length
The total length of the inflorescence usually measured from the proximal end of the peduncle to the distal end of the growing apex
InfL
Inflorescence
length
gram per plant
Acid detergent lignin measurement
Description: Measurement of acid detergent lignin with acid detergent solution
Materials: See protocol
Dependent_on:
Protocol:
1. See https://uwlab.soils.wisc.edu/wp-content/uploads/sites/17/2015/09/forage_lignin.pdf
number per raceme
Flower number
The total number of flowers counted, estimated, or observed.
FlN
Flower
number
centimeter
Legume fruit color
The average color of a legume fruit or group of legume fruits from a plant, expressed as a vector of float values in the color model of choice such as RGB or HSV.
FrCol
Legume fruit
color
Seedling emergence date estimation
Description: Estimate the emergence date of the shoots of seedlings
Materials:
Dependent_on:
Protocol:
1. Record the date (ISO8601 format) when at least 50% seedlings in a plot have germinated and are starting to break through the soil surface (establishment year)
Seed total starch content measurement
Description: Determination of total starch by amyloglucosidase/alpha-amylase method
Materials:
Dependent_on:
Protocol:
1. See full protocol at AOAC 996.11
Seedlot germination
The germination rate of a seedlot
SLG
Seedlot
germination
Flowering percentage computation
Description: Compute the percentage of plants in a plot that are flowering
Materials:
Dependent_on: Plant number cnt [n]
Protocol:
1. Count the number of plants in a plot that have at least one flower.
2. Divide by the total number of plants in the plot.
3. Express as a percentage.
Percent dry matter computation
Description: Compute the dry matter percentage of a sample
Materials:
Dependent_on: Forage fresh mass msr [kg], Forage dry mass msr [kg] | Forage subsample fresh mass msr [kg], Forage subsample dry mass msr [kg]
Protocol:
1. Divide the dry biomass by the fresh biomass and multiply by 100 to get the percent dry matter of either a sample of subsample of forage.
method_of
Raceme number per plant computation
Description: Compute the number of racemes per plant
Materials:
Dependent_on: Raceme number cnt [n], Plant number cnt [n]
Protocol:
1. Sample plants according to your sampling protocol
2. Set aside each stem on each plant and quickly tally all the racemes
3. Record the total number of racemes counted and divided by the total number of plants harvested/sampled.
gram per stem
Forage ash content
The total ash content
FoAC
Forage
ash content
Seed insoluble dietary fiber content
The dietary fiber content in the insoluble fraction of a sample of seeds.
SdIDFC
Seed
insoluble dietary fiber content
Flower number per raceme computation
Description: Count the total number of flowers or flower scars that are present on a raceme.
Materials: loupe (if needed);
Dependent_on: Flower number cnt [n], Raceme number cnt [n]
Protocol:
1. Select racemes according to your sampling protocol.
2. Count and record the number of flowers or flower scars on each raceme.
3. Record the average number of leaves per raceme.
Defoliation date measurement
Description: Record the date at which a plant or plot was defoliated
Materials:
Dependent_on:
Protocol:
1. Record the ISO 8601 date at which a plant or plot was defoliated
Stem erectness estimation
Description: Estimate the erectness of stems in a plant or plot of plants
Materials:
Dependent_on:
Protocol:
1. Estimate the percentage of stems that have a mean angle greater than 45 degrees from the ground.
2. Record the stem erectness as the number bin (0-9) that contains the estimated percentage.
hectare
scale_of