KAUST StmLy_E_1to9 StmLy Clara Stanschewski Clara Stanschewski PcleShp PcleShp_E_1to3 KAUST Clara Stanschewski GH GH_E_1to7 KAUST SdWpPlot KAUST SdWpPlot_M_g Clara Stanschewski PtHt_M_cm Clara Stanschewski PtHt KAUST KAUST Clara Stanschewski PcleAng_E_1to7 PcleAng KAUST BGDBM_M_g Clara Stanschewski BGDBM AxlPig_E_0to1 KAUST Clara Stanschewski AxlPig SdPrm_Comp_mm Clara Stanschewski SdPrm KAUST KAUST PcleCol_E_4to16 PcleCol Clara Stanschewski SdAvgGreen_Comp_rgb Clara Stanschewski SdAvgGreen KAUST Quinoa ontology PcleDen KAUST Clara Stanschewski PcleDen_E_1to7 SdGerm_M_pct KAUST SdGerm Clara Stanschewski Clara Stanschewski PltNoHvRp PltNoHvRp_Ct_plant KAUST Clara Stanschewski GrStg KAUST GrStg_E_BBCH KAUST StmLodg Clara Stanschewski StmLodg_E_1to9 SdA Clara Stanschewski SdA_Comp_mm KAUST Clara Stanschewski SdWpPt_C_g SdWpPt KAUST SdAvgRed Clara Stanschewski KAUST SdAvgRed_Comp_rgb Clara Stanschewski HI_C_pct KAUST HI LfMgn KAUST Clara Stanschewski LfMgn_E_1to5 PlotHomo_E_1to7 Clara Stanschewski PlotHomo KAUST KAUST PlotCov_E_1to9 Clara Stanschewski PlotCov Brnch_E_1to7 KAUST Brnch Clara Stanschewski StmCol_E_4to15 KAUST StmCol Clara Stanschewski StmDiaBse_M_mm Clara Stanschewski KAUST StmDiaBse KAUST Clara Stanschewski SdSht SdSht_E_1to7 KAUST StmAng_E_1to7 StmAng Clara Stanschewski Clara Stanschewski SdWRepPt_M_g SdWRepPt KAUST PcleLng Clara Stanschewski PcleLng_M_cm KAUST Clara Stanschewski StmBrk_E_1to9 StmBrk KAUST KAUST Clara Stanschewski StmDiaPcle_M_mm StmDiaPcle entity KAUST AGDBM Clara Stanschewski AGDBM_M_g KAUST SigPcle_Ct_panicle Clara Stanschewski SigPcle KAUST SdHlW SdHlW_M_g Clara Stanschewski Clara Stanschewski TGW KAUST TGW_M_g KAUST Clara Stanschewski PltNoHv_Ct_plant PltNoHv PcleLfs_E_1to7 Clara Stanschewski KAUST PcleLfs StmStr KAUST StmStr_E_0to1 Clara Stanschewski StmLfShp_E_1to2 KAUST StmLfShp Clara Stanschewski KAUST SdAvgBlue_Comp_rgb Clara Stanschewski SdAvgBlue attribute acronym Stem striae estimation None Thousand grain weight TGW Seed Thousand Grain Weight Thousand Grain Weight (TGW), the weight of 1000 seeds Plot population homogeneity estimation None Height of the most representative plants of the plot, usually from the middle of the plot, measured with a long measuring stick from soil to the tip of the panicle. If more than one distinct phenotype is present, more than one plant may be recorded in a new row of the spreadsheet, with all phenotypes that are differing recorded separately PtHt Plant Plant height Height Shattering SdSht Seed shattering Grain persistence in the plant at physiological maturity. Assessing how easy seeds fall off the panicle upon light touch: 1 = No seeds falling to 7 = a lot of seeds falling Seed Phenological growth stage; very important to record at mature phenotyping Plant GrStg Growth stage Growth stage Stem breakage incidence estimation None rgb Leafiness Panicle leafiness Panicle PcleLfs Scored from 1 = no leaves to 7 = many leaves BBCH Plants are prostrate, on or near the ground, with intact stems; assessing the percentage of the plot affected Stem Stem lodging incidence Lodging incidence StmLodg Stem diameter near plant base measurement None None Seed yield per plant computation Seed yield per plot measurement None None Stem lying incidence estimation None Seed yield for representative plants measurement Thickness of the stem measured just underneath the main panicle Stem diameter under the panicle Stem Diameter under the panicle StmDiaPcle Axil pigmentation estimation None Seed Average area of the seeds from image analysis average area SdA Seed average area Plot population homogeneity Plot Population homogeneity PlotHomo Judgment of homogeneity of the accession, 1 = homogeneous to 7 = mixed Seed average area computation None Count of the number of significant panicles, i.e., larger panicles, near the top of the plant, harvestable, that provide a major contribution to the seed harvested from the plant SigPcle Number of significant Panicle Number of significant panicles method_of The angle at which the majority of panicle axes are leaning, measured between an upright panicle on the verticle and a panicle pointing towards the ground PcleAng Panicle axis angle Panicle Axis angle Stem angle estimation None Stem StmCol Stem color Color Categorized into green (13), red (4), or no pigmentation (15) Panicle shape 1-3-5 SdWpPlot Seed Yield total Harvesting the panicles remaining per plot while excluding borders, and adding the weight to that from the four representative plants, seed dried in oven to constant weight Seed yield per plot Growth stage estimation None Stem Angle The angle at which the majority of plants are leaning, measured between the vertical axis and the horizontal axis StmAng Stem angle None Leaf margin estimation Seed germination rate measurement None Plant branchiness 1-3-5-7 average perimeter Average perimeter of the seeds from image analysis Seed average perimeter SdPrm Seed Thousand Grain Weight measurement None If possible, root biomass could also be measured (especially when plants are growing in sandy soil) Plant Plant below-ground dry biomass BGDBM Below-ground dry biomass Pigmentation Axil pigmentation Axil Presence (1) absence (0) scoring of pigmented axils AxlPig Lying incidence Stem of the plant is not emerging straight up from the soil but has a kink at the base, growing along the ground before rising; assessing the percentage of the plot affected Stem lying incidence StmLy Stem Leaf margins are classified into 3 groups Leaf Leaf margin LfMgn Margin Seed shattering estimation None None Stem diameter under the panicle measurement SdGerm Tested in petri dishes (%) Germination rate Seed germination rate Seed None Number of significant panicles counting Coverage Plot Plot coverage PlotCov Percentage of the plot covered, 1 = poor to 7 = good establishment Stem breakage incidence StmBrk Stem Breakage incidence Stems are broken or detached, assessing the percentage of the plot affected Total harvested seed mass per plant may be calculated from number of plants harvested and the seed mass of all plants in the plot (SdWtpPlot/PltNoHv) SdWpPt Seed Yield per plant Seed yield per plant Striae Stem striae Presence (1) absence (0) scoring of stem streaks or stripes StmStr Stem Panicle density 1-3-5-7 Stem angle 1-3-5-7 Seed shattering 1-3-5-7 PcleShp Panicle shape Shape Panicle Classified into one of the three categories: glomerulate, intermediate, or amarantiform PltNoHv Number of plants harvested per plot Plants harvested Plot Total number of plants that were harvested of the plot. mm cm % Plant below-ground dry biomass measurement None Panicle length measurement None None Seed average green color computation plant None Stem leaf shape estimation g Stem breakage incidence 1-3-5-7-9 Yield of representative plants Seed yield for representative plants Seed SdWRepPt Seed mass of approximately four representative plants that were harvested from the center of the plot (seed should be dried to constant weight) Panicle leafiness estimation None Branchiness Plant Brnch Plant branchiness Score for the overall amount of side branches of representative plants in a plot, ignoring very small and spindly branches, ranging from 1 = no branches to 7 = bushy plant with many major lateral branches Axil pigmentation 0-1 Seed average red color computation None StmLfShp Stem Stem leaf shape Leaf shape Leaves of the stem are categorized into two groups: rhomboidal (1) and triangular (2) Thickness of the stem measured with calipers at the middle of the bottom third of the plant stem Stem diameter near plant base Diameter at the plant base StmDiaBse Stem None Panicle shape estimation Leaf margin 1-3-5 Seed average red color Numerical seed color red value, RGB R Component from image analysis average red color SdAvgRed Seed Seed hectoliter weight SdHlW Seed Hectoliter weight Hectoliter weight, or specific weight of seeds: Estimation of density, determined by weighing all seeds fitting into a 100 ml volume panicle Panicle leafiness 1-3-5-7 Plant growth habit estimation None scale_of Count the number of plants that were harvested seperately from the majority of the plot, as representative plants. PltNoHvRp Number of representative plants harvested Plot Representative plants harvested Panicle Panicle color PcleCol Categorized according to the color scoring card Color Seed average blue color Seed average blue color Numerical seed color blue component, RGB B Component from image analysis SdAvgBlue Stem lying incidence 1-3-5-7-9 Stem color 13-4-15 Seed Seed average green color Numerical seed color green value, RGB G Component from image analysis SdAvgGreen average green color None Panicle color estimation Seed average perimeter computation None None Seed average blue color computation Above-ground dry biomass AGDBM Cutting plants at the very base with secateurs and drying the entire above-ground section of the plant. Recording total dry weight and number of plants that make up total dry weight. Divide total weight with number of plants to give weight per plant Plant above-ground dry biomass Plant Harvest index computation None Plant growth habit 1-3-5-7 Panicle Length of the primary panicle measured with the same stick. Measured from the base of the panicle to the tip Length Panicle length PcleLng Number of plants harvested per plot counting None None Plot coverage estimation None Plant above-ground dry biomass measurement Stem leaf shape 1-2 None Panicle axis angle estimation GH Plant growth habit Growth habit Four categories of growth habit described in images on the scoring card. Here the focus lies on whether branching is present from the base of the plant and if a main inflorescence can be identified Plant Plot population homogeneity 1-3-5-7 Yield/ above-ground biomass Plant HI Harvest index Havest index Stem striae 0-1 Plot coverage 1-3-5-7-9 Panicle density estimation None Number of representative plants harvested counting None Plant height measurement None Seed hectoliter weight measurement None None Plant branchiness estimation Density PcleDen Panicle density Panicle Scored from 1 = lax (loose) with panicle axes easily visible to 7 = tight and compact panicles Stem lodging incidence estimation None Stem lodging incidence 1-3-5-7-9 Panicle axis angle 1-3-5-7 Panicle color 13-16-4-7-15 None Stem color estimation variable_of