PhomaSKbase_E_0to7 Yongju Huang, John West University of Hertfordshire Sdy-toc_M_mgkg University of York Swen Langer, Lenka Havlickova Richard Broughton, Frederic Beaudoin SdCampesterol_M_mgkg Rothamsted Yongju Huang, John West LLSpods_E_pct University of Hertfordshire Nottingham Martin Broadley,Thomas Alcock LfK_M_mgkg 1 trace linoleicSeedCont_M_pct Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC Anne Laperche INRA Nottingham Martin Broadley, Catherine Thomas MLRL_Cp_cm LfNa_M_mgkg Martin Broadley,Thomas Alcock Nottingham 3 SdZn_M_mgkg Thomas Alcock, David Salt Nottingham stageG5_cnt_dayj Growth Stage G5;10% of the seeds have reached maturity Anne Laperche INRA seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5 Martin Broadley,Thomas Alcock LfS_M_mgkg Nottingham elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2 Growth Stage F2;10% of the flowers are open on the main inflorescence stageF2_cnt_dayj Anne Laperche INRA Anne Laperche Hail Damage;HAILDMG INRA hailDmg_E_0to9 VertWilt_E_0or1 Yongju Huang, John West University of Hertfordshire No leaf spotting 0 Anne Laperche seedMoist_mlab_pct INRA Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB Thomas Alcock, David Salt Nottingham SdK_M_mgkg Thomas Alcock, David Salt Nottingham SdNa_M_mgkg PhomaLS_Cou_plnt10plnt Yongju Huang, John West University of Hertfordshire PhomaSKupper_E_0to7 Yongju Huang, John West University of Hertfordshire 75-100% girdling 5 PhomaSK_E_0to7 University of Hertfordshire Yongju Huang, John West Yongju Huang, John West LeafMiner_Cou_pct University of Hertfordshire PodNumbTermRac_Ct_PodTermRac John Innes Centre Rachel Wells LfCr_M_mgkg Nottingham Martin Broadley,Thomas Alcock SdCo_M_mgkg Thomas Alcock, David Salt Nottingham Anne Laperche INRA Plant Density;Number of viable plants counted per surface unit;PLANTDENS plntStnd_M_plntm2 John Innes Centre Rachel Wells PodLen_M_cm Anne Laperche INRA linolenicSeedCont_M_pct Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC Anne Laperche Crop problems;Crop problems during the cycle.;CROP cropAnnot_E_cmt INRA Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI INRA Anne Laperche NHI_Comp_pct Thomas Alcock, David Salt SdMo_M_mgkg Nottingham Biotic Stress;Biotic attack during the crop cycle;BIOTIC bioticAnnot_an_text Anne Laperche INRA Richard Broughton, Frederic Beaudoin SdAlk_M_mgkg Rothamsted Anne Laperche INRA plntStdEmerg_M_plt Plant Emergence;Percentage of seeds that emerge;EMERG University of Hertfordshire PowMildew_E_0or1 Yongju Huang, John West University of Hertfordshire LLSstem_E_pct Yongju Huang, John West oleicSeedCont_M_pct Anne Laperche Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC INRA Nottingham LfSr_M_mgkg Martin Broadley,Thomas Alcock 3 low damage Yongju Huang, John West University of Hertfordshire white blight Sclerot_Cou_plntPlot Anne Laperche Growth Stage C1;Bolting date when the first inter-node appears INRA stageC1_cnt_dayj corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1 SdStigmasterol_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted LfAl_M_mgkg Nottingham Martin Broadley,Thomas Alcock seedMoistHarv_mhumi_pct Anne Laperche INRA Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV Martin Broadley, Catherine Thomas Nottingham LRL_M_cm Martin Broadley,Thomas Alcock LfMg_M_mgkg Nottingham Martin Broadley,Thomas Alcock LfV_M_mgkg Nottingham Nottingham SdS_M_mgkg Thomas Alcock, David Salt oilYield_Comp_qha Anne Laperche INRA Oil Yield;Oil yield per ha;OY Nottingham SdCr_M_mgkg Thomas Alcock, David Salt 8 University of Hertfordshire Yongju Huang, John West LLSleaf_E_0to7 Thomas Alcock, David Salt SNi_M_mgkg Nottingham Yongju Huang, John West PhomaLS_Cou_pct University of Hertfordshire stageG4_cnt_dayj Anne Laperche INRA Growth Stage G4;30% of the pods have reached their final size flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4 Thomas Alcock, David Salt SdMg_M_mgkg Nottingham Rothamsted SdAld_M_mgkg Richard Broughton, Frederic Beaudoin FertBraNumb_Ct_Fb Rachel Wells John Innes Centre Rothamsted SdB-Sitosterol_M_mgkg Richard Broughton, Frederic Beaudoin SdBrassicasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdFA_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Rothamsted SdCycloartenol_Cp_pct Richard Broughton, Frederic Beaudoin Nottingham Martin Broadley,Thomas Alcock LfSe_M_mgkg Anne Laperche oilSeedCont_M_pct INRA Oil %;Content of oil in seeds determined by NIRS;O Anne Laperche Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC INRA climaticAnnot_E_cmt Nottingham Martin Broadley,Thomas Alcock LfB_M_mgkg 2-25% girdling 2 SdBranched_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted John Innes Centre Rachel Wells PedLen_M_cm plotHarvest_M_kg Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST Anne Laperche INRA LfCa_M_mgkg Nottingham Martin Broadley,Thomas Alcock University of York Sda-toc_M_mgkg Swen Langer, Lenka Havlickova Nottingham Martin Broadley,Thomas Alcock LfCd_M_mgkg PhomaLS_E_0to3 Yongju Huang, John West University of Hertfordshire 6 intermediate to high damage Richard Broughton, Frederic Beaudoin SdBrassicasterol_Cp_pct Rothamsted high damage 7 Richard Broughton, Frederic Beaudoin SdCycloartenol_M_mgkg Rothamsted INRA Anne Laperche seedMoist_mnirs_pct Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS Prot Yield;Protein yield per ha;PrY Anne Laperche INRA protYield_Comp_qha Nottingham Martin Broadley,Thomas Alcock LfNi_M_mgkg Plot Surface;Surface of the elementary experimental plot;PLOTSURF INRA plotSize_M_m2 Anne Laperche SdCu_M_mgkg Thomas Alcock, David Salt Nottingham Yongju Huang, John West LLSleaf_E_pct University of Hertfordshire PltHght_M_cm John Innes Centre Rachel Wells stageF1_cnt_dayj Growth Stage F1;First flowers are open Anne Laperche INRA corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1 Thomas Alcock, David Salt Nottingham SdCa_M_mgkg 4 INRA Annotations on measure validity;VALIDATION validateannot_E_cmt Anne Laperche SdMn_M_mgkg Thomas Alcock, David Salt Nottingham 3 26-50% girdling moderately regular 5 Rachel Wells StemWid_M_cm John Innes Centre SdLi_M_mgkg Thomas Alcock, David Salt Nottingham 1 high > 90% erucicSeedCont_M_pct Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC Anne Laperche INRA 5 intermediate damage Nottingham SdSr_M_mgkg Thomas Alcock, David Salt Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO INRA stageFlo_cnt_dayj Anne Laperche Anne Laperche INRA Phoma damage G2 score phomaDmg_CompG2_0to9 BeakLen_M_cm John Innes Centre Rachel Wells Nottingham Martin Broadley,Thomas Alcock LfFe_M_mgkg stagePodDev_cnt_dayj Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV Anne Laperche INRA John Innes Centre Rachel Wells FtLen_M_cm Thomas Alcock, David Salt Nottingham SdB_M_mgkg 4 51-75% girdling 2 Culture leaning, without impact 0 no symptoms observable Nottingham Martin Broadley,Thomas Alcock LfBa_M_mgkg Anne Laperche Plant Lodging;Scoring of the plant lodging of the plot;LODGING INRA lodging_E_0to9 SdPb_M_mgkg Thomas Alcock, David Salt Nottingham Nottingham Martin Broadley,Thomas Alcock LfMn_M_mgkg LfAs_M_mgkg Nottingham Martin Broadley,Thomas Alcock high irregular 1 Rothamsted SdCampesterolDiunsat_M_mgkg Richard Broughton, Frederic Beaudoin 4 Diseased leaved with a few large lesions of many small leasions, area quite heavily infected Nottingham SdCd_M_mgkg Thomas Alcock, David Salt white blight Yongju Huang, John West Sclerot_Cou_plnt6plnt University of Hertfordshire Brassica ontology Brassica napus ontology LfPb_M_mgkg Nottingham Martin Broadley,Thomas Alcock SdAlcII_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdSe_M_mgkg Thomas Alcock, David Salt Nottingham SdPhytosterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted very low damage 2 stageEndFlo_cnt_dayj INRA Anne Laperche Growth Stage End Flowering;End of flowering on the main inflorescence corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO Anne Laperche INRA Pod shattering;Scoring of pods open just before harvest;PODSHAT podShat_E_score Growth Stage G1;10% of the pods have reached their final size flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1 stageG1_cnt_dayj Anne Laperche INRA Average loding 5 regStand_E_0to9 Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND Anne Laperche INRA Rothamsted SdAvenasterol_Cp_pct Richard Broughton, Frederic Beaudoin LfP_M_mgkg Nottingham Martin Broadley,Thomas Alcock Rothamsted Richard Broughton, Frederic Beaudoin SdKeto_M_mgkg Thomas Alcock, David Salt SdP_M_mgkg Nottingham Martin Broadley,Thomas Alcock Nottingham LfRb_M_mgkg Protein %;Content of proteins in seeds determined by NIRS;Pr Anne Laperche protSeedCont_M_pct INRA 7 100% girdling (stem dead/lodged)) 6 LfZn_M_mgkg Nottingham Martin Broadley,Thomas Alcock palmiticSeedCont_M_pct Anne Laperche Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC INRA Martin Broadley,Thomas Alcock LfAg_M_mgkg Nottingham Nottingham Martin Broadley, Catherine Thomas LRD_Cp_rootcm Richard Broughton, Frederic Beaudoin Rothamsted SdB-Sitosterol_Cp_pct stearicSeedCont_M_pct Anne Laperche INRA Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC <5 spots per plant 1 seedImpur_M_pct Anne Laperche INRA Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR Nottingham TRL_Cp_cm Martin Broadley, Catherine Thomas protMealCont_M_pct Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL Anne Laperche INRA SdTotalWax_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Rachel Wells PodWid_M_cm John Innes Centre Nottingham LfCs_M_mgkg Martin Broadley,Thomas Alcock Rothamsted SdCampesterol diunsat_Cp_pct Richard Broughton, Frederic Beaudoin 4 LfMo_M_mgkg Nottingham Martin Broadley,Thomas Alcock Nottingham Martin Broadley, Catherine Thomas PRL_M_cm 3 60-90% Thomas Alcock, David Salt Nottingham SdRb_M_mgkg irregular 3 6 100% girdling ( stem weak/firm) NA 0 glsSeedCont_M_μmolg INRA Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS Anne Laperche LfTi_M_mgkg Nottingham Martin Broadley,Thomas Alcock SdNum_Ct_sd John Innes Centre Rachel Wells 2 Diseased leaves with non or a few small lesions Rothamsted Richard Broughton, Frederic Beaudoin SdCampesterol_Cp_pct Nottingham SdAs_M_mgkg Thomas Alcock, David Salt No disease observable 0 severe damage 9 Swen Langer, Lenka Havlickova Sdd-toc_M_mgkg University of York LfCu_M_mgkg Nottingham Martin Broadley,Thomas Alcock 0 NA VertWilt_Cou_pct University of Hertfordshire Yongju Huang, John West attribute Richard Broughton, Frederic Beaudoin Rothamsted SdStigmasterol_M_mgkg acronym 5 Half green/half diseased, leaf curling and distortion 1 No lodging Nottingham Martin Broadley, Catherine Thomas SD_Est_mm 7 10-30% 0 NA Thomas Alcock, David Salt Nottingham SdFe_M_mgkg John Innes Centre Rachel Wells SdWght_M_mg 2 5-10 spots per plant Nottingham Martin Broadley, Catherine Thomas LRN_Ct_root 30-60% 5 LfU_M_mgkg Nottingham Martin Broadley,Thomas Alcock 1 ≤ 5% girdling Plant Size;Height of plants in an experimental plot;PLANTSIZE plntHeight_M_cm INRA Anne Laperche stagePodMaturity_cnt_dayj Anne Laperche Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY INRA low to intermediate damage 4 9 perfectly regular Richard Broughton, Frederic Beaudoin SdAvenasterol_M_mgkg Rothamsted regular 7 2 no visible affection 0 3 Plants with scattered lesions high to severe damage 8 Trace of disease 1 Rothamsted Richard Broughton, Frederic Beaudoin SdAlcI_M_mgkg Weevils (all species) damage score;charancon weevilsDmg_E_0to9 Anne Laperche INRA 6 Evidence of stunting 7 Plants heavily stunted or dead. < 10% 9 entity 3 >10 spots per plant Comment GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement * 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.). * Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers. * Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C. * Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. * After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS. * GC program: * Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes. * Inlet temp: 280°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C BBCH growth stage 60;CETIUM growth stage F1 First flowers time (BBCH 60) First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale) Development stage Plant SdNa Seed Seed Sodium content Sodium content The concentration of Sodium in seeds B-Sitosterol SdB-Sitosterol Seed The concentration of B-Sitosterol in seeds B-Sitosterol content Seed B-Sitosterol content SdFe The concentration of Iron in seeds Seed Seed Iron content Iron content Pod width width Pod width of pods from terminal raceme pod Plant Lodg Lodging incidence The plant lodging incidence. Plant lodging incidence SdAs The concentration of Arsenic in seeds Seed Arsenic content Seed Arsenic content Stem base Phoma stem canker base stem score Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity mg/kg plants/10 plants The concentration of Rubidium in seeds SdRb Seed Rubidium content Rubidium content Seed Plant Flowering time (BBCH 62-65) flowering stage;time to BBCH growth stage 62-65;Anthesis Additional flowering stages according to the BBCH scale (62-65) Development stage length Lateral root Lateral root length Length of the lateral root LRL LfAg Leaf Silver content Leaf Silver content The concentration of Silver in leaves MlProt The concentration of proteins in the meal. Protein content Meal protein content Meal SdK The concentration of Potassium in seeds Seed Potassium content Seed Potassium content TRL Total root length Length of all roots length Total root Relative seed Phytosterol content - Computation The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols Plant Early pod development time (BBCH 73) BBCH growth stage 73;CETIUM growth stage G4 Development stage 30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale) Height is measured from ground to the top of the plants using a dipstick graduated in cm Plant height - Measurement Scoring of the regularity of the plant stand Plant Stand Regularity of plant stand Seed Cobalt content SdCo The concentration of Cobalt in seeds Cobalt content Seed RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN). Image analysis - Counting A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents HPLC (High performance liquid chromatography) analysis - Measurement Number of fertile branches Number of fertile branches fertile branch number NIRS assay. Pct of Seed Dry Weight Protein meal content NIRS - Measurement Light Leaf spot severity on leafs Light leaf spot resistance LLS severity on leaf Leaf Campesterol diunsat proportion of Phytosterols Seed diunsaturated Campesterol content Campesterol diunsat Seed SdCampesterolDiunsat_Cp The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of pod development (BBCH 71) date – Estimation Additional stages for pod development according to the BBCH scale (72-79) Development stage Pod development time (BBCH 72-79) pod development stage;time to BBCH growth stages 72-79 Plant NIRS assay. Pct total fatty acids Erucid seed content NIRS - Measurement The concentration of Cadmium in seeds Cadmium content SdCd Seed Cadmium content Seed Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image. Stem witdh - Measurement Leaf Leaf Sulphur content Sulphur content LfS The concentration of Sulphur in leaves Brassicasterol proportion of Phytosterols The percentage of Brassicasterol in the total of Phytosterols in seeds Seed Brassicasterol content Brassicasterol SdBrassicasterol Seed cm The concentration of Calcium in seeds SdCa Calcium content Seed Calcium content Seed Phoma damage PhomaDmg Withering disease caused by the agent Leptosphaeria maculans (Phoma). Withering disease Plant Phoma damage Plant Weevils damage WeevilsDmg Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil). Weevils damage Seed delta- tocopherol content Delta- tocopherol content in seeds seed delta-tocopherol content Sdd-toc Observe damage caused by Weevils (all species), and rate the damage. Weevil damage - Estimation Leaf The concentration of Aluminium in leaves Leaf Aluminium content LfAl Aluminium content Rubidium content Leaf Rubidium content The concentration of Rubidium in leaves LfRb Leaf Phoma stem canker severity Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity Stem 3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry Leaf mineral analysis - Measurement The percentage of Cycloartenol in the total of Phytosterols in seeds Cycloartenol Seed Cycloartenol proportion of Phytosterols Seed Cycloartenol content SdCycloartenol_Cp The concentration of Manganese in seeds Seed Manganese content Manganese content SdMn Seed Plant The number of plants that showed presence of phoma leaf spots from a subsample of a plot Number of plants with phoma leaf spots Phoma leaf spots CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of pod maturity (BBCH 81) date – Estimation Oil yield Oil yield Oil yield Plant OY height plant Maximum plant height from ground to tip of terminal raceme Plant height PH Leaf Arsenic content LfAs Leaf Arsenic content The concentration of Arsenic in leaves Seed The concentration of Avenasterol in seeds SdAvenasterol Avenasterol content Seed Avenasterol content Avenasterol CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First flowers (BBCH 60) date – Estimation Leaf The concentration of Boron in leaves Boron content LfB Leaf Boron content boolean Alkane-functional group content The concentration of components with alkane functional groups from pod and stem material Pod and stem Pod and stem Alkane-functional group content SdAlk Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively Seed moisture content – Computation Surface of the elementary experimental plot Plot PlotSize Size Plot size stem width Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant Stem width Visual notations (scale 0 - 9) Pod shattering - Estimation NIRS assay. Pct total fatty acids Stearic seed content NIRS - Measurement The concentration of Molybdenum in seeds SdMo Seed Seed Molybdenum content Molybdenum content gamma-tocopherol content Seed gamma- tocopherol content Sdy-toc seed Gamma- tocopherol conten in seeds Hail damage Plant HailDmg Plant damage caused by hail. Hail damage Lateral root MLRL The mean length of a lateral root of that root system Mean lateral root length average length Climatic incidents annotions - Estimation Visual observations Leaf Leaf miner incidence The percentage of plants that showed presence of leaf miner from a subsample of a plot Incidence with leaf miner 10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated. LLS severity - Estimation 0-7 disease stage scale Samples from HI assay are analysed for N_%DW in the different fractions Nitrate Harvest index - Computation seed Seed weight Seed weight of ten typical pods weight Leaf Manganese content LfMn Leaf The concentration of Manganese in leaves Manganese content Seed Campesterol diunsat The concentration of diunsaturated Campesterol in seeds SdCampesterolDiunsat diunsaturated Campesterol content Seed diunsaturated Campesterol content Leaf LfTi Leaf Titanium content Titanium content The concentration of Titanium in leaves LfCr Leaf Chromium content Chromium content Leaf The concentration of Chromium in leaves Visual observations Biotic stress annotations - Estimation 3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg Seed mineral analysis - Measurement NHI Nitrogen Harvest Index Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root) Seed N content to total plant N content ratio N content proportion of plant N content Seed % Oil seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity Phoma stem canker upper stem lesion score Upper stem Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Powdery mildew presence Plant Absence or presence of powdery mildew Presence (1) or absence (0) of powdery mildew SdGLS Seed Seed glucosinolate content The concentration of glucosinolates in seeds. Glucosinolate content Avenasterol Seed The percentage of Avenasterol in the total of Phytosterols in seeds Avenasterol proportion of Phytosterols Seed Avenasterol content SdAvenasterol_Cp Primary root lengh Primary root length Length of the primary root PRL Stand Plant Plant stand Crop stand Plant density Number of viable plants counted per surface unit PltSd root/cm Biotic attack during the crop cycle. Biotic attack annotation Plant BioticPb Exposure to biotic attack; damage du to biotic attack Verticilium wilt presence - Estimation Scoring of presence or absence of verticilium wilt symptoms across whole plant. foot Length of lower stem from ground level to last formed fertile branch Foot length length LRN Lateral root number Number of lateral roots number Lateral root Pod and stem The concentration of components with secondary alcohol functional groups from pod and stem material secondary Alkohol functional group content SdAlcII Pod and stem secondary Alkohol functional group content CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Addictional flowering stages (BBCH 62-65) date – Estimation Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation Alpha- tocopherol content in seeds Seed alpha- tocopherol content alpha-tocopherol content seed Sda-toc Stem length - Measurement Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Leaf Leaf Magnesium content The concentration of Magnesium in leaves LfMg Magnesium content GLS seed content NIRS - Measurement NIRS assay Quantity per Seed Dry Weight Seed protein yield Protein yield PrY Protein yield per ha Seed Plant Sclerotinia incidence No of plants with Sclerotinia (per plot) Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. Pod number pod Number of pods on terminal raceme number Plant Crop problems annotation Exposure to crop problems;damage from crop problem CropPb Crop problems during the crop cycle. The concentration of Caesium in leaves Caesium content Leaf Caesium content LfCs Leaf SdBrassicasterol Seed The concentration of Brassicasterol in seeds Brassicasterol content Seed Brassicasterol content Brassicasterol SdImpur Impurities content Percentage of impurities in a sample of seeds determined by weighing. Seed impurity content Seed Fertile Branches - Counting Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Seed Nickel content The concentration of Nickel in seeds Seed Nickel content SdNi q/ha Shattering Pod Pod shattering Proportion of open pods (assessment) PodShat density The mean density of lateral roots along the primary root Lateral root density LRD Lateral root The concentration of Zinc in leaves LfZn Leaf Zinc content Leaf Zinc content Plant Verticilium wilt presence Absence or presence of Verticilium wilt on the plant Verticilium wilt presence Sclerotinia incidence (diseased) - Counting The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded. Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Leaf The concentration of Uranium in leaves LfU Uranium content Leaf Uranium content * Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation. * Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step. * 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples. * Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour. * Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials. * A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions. * The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes. * 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used). * The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane. * Samples are vortexed, centrifuged and the upper hexane phase is removed. * A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first. * Samples are dried in a rotary evaporator or under a stream of nitrogen. * Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID). *GC program: * Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes. * Inlet temp: 325°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement Phoma leaf spot severity Severity assessment of phoma leaf spot Leaf phoma resistance Leaf Light leaf spot incidence Leaf LLS incidence on leaf The percentage of plants that showed presence of light leaf spot incidence on leaves The concentration of Balium in leaves Balium content Leaf Leaf Balium content LfBa Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod width - Measurement Plant stand at emergence – Computation EMERG = (Number of plants obtained / Number of seeds sown) x 100 The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation wax content Pod and stem wax content SdTotalWax Pod and stem The concentration of wax in the pod and seed material Vanadium content Leaf LfV Leaf Vanadium content The concentration of Vanadium in leaves Seed number seed Number of seeds in ten typical pods number fertile branch Leaf LfCd The concentration of Cadmium in leaves Leaf Cadmium content Cadmium content Leaf LfFe The concentration of Iron in leaves Leaf Iron content Iron content The concentration of Potassium in leaves Leaf Potassium content Leaf LfK Potassium content Exposure to climatic stress Plant ClimPb Climatic incidents annotation Climatic incidents during the crop cycle. Evaluation of the Phoma lingam infection with the G2 score. The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation. Phoma damage – G2 score – Computation Linoleic acid content Seed linoleic acid content Seed StLinoleic The concentration of linoleic acid in seeds. SdMoist Seed moisture content. Seed moisture content Moisture content Seed 2020-10-19T00:00:02 Seed Number - Counting Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Pod length of pods from terminal raceme Pod length pod length LLS severity - Estimation 10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated. SdZn Zinc content The concentration of Zinc in seeds Seed Zinc content Seed Pod beak length - Measurement Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant. Copper content Seed Seed Copper content The concentration of Copper in seeds SdCu Plant Verticillium wilt incidence Verticilium wilt presence The percentage of plants that showed presence of leaf miner from a subsample of a plot Visual notations (scale 0 - 9) regularity stand - Estimation SdB-Sitosterol_Cp The percentage of B-Sitosterol in the total of Phytosterols in seeds Seed B-Sitosterol content B-Sitosterol B-Sitosterol proportion of Phytosterols Seed BBCH growth stage 81;CETIUM growth stage G5 Plant Development stage Beginning of pod maturity time (BBCH 81) 10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale) Plant height - Measurement Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. The concentration of Nickel in leaves Nickel content Leaf Nickel content LfNi Leaf Powdery mildew presence - Estimation Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1 Seed diameter SD Seed diameter The diameter of a seed plants/plot Seed oleic acid content The concentration of oleic acid in seeds. Oleic acid content Seed StOleic Seed Stigmasterol proportion of Phytosterols SdStigmasterol_Cp Stigmasterol Seed Sigmasterol content The percentage of Stigmasterol in the total of Phytosterols in seeds Beginning of pod development time (BBCH 71) BBCH growth stage 71;CETIUM growth stage G1 Plant 10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale) Development stage Seed The concentration of Lithium in seeds Seed Lithium content SdLi Lithium content 0-9 Regularity scale Boron content The concentration of Boron in seeds Seed Boron content SdB Seed CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First inter-node (BBCH 31) date – Estimation Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr) Seed protein Yield - Computation Plant BBCH growth stage 69;end flowering stage End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale) Development stage End of flowering time (BBCH 69) 0-5 percentage bracket scale SdOil Seed Seed oil content The concentration of oil in seeds. Oil content SdCr Chromium content The concentration of Chromium in seeds Seed Chromium content Seed Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation The concentration of Sulphur in seeds Seed Sulphur content SdS Sulphur content Seed Image analysis - Computation The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001) Plant 10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale) Development stage BBCH growth stage 61;CETIUM growth stage F2 Beginning of flowering time (BBCH 61) plant/m2 Yield Weight (kg) of raw materials harvested by plot Biomass yield Biomass A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester plotharvest - Measurement Leaf The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot Phoma incidence Incidence of Phoma leaf spot NIRS assay Seed moisture NIRS - Measurement Seed Campesterol content The percentage of Campesterol in the total of Phytosterols in seeds Campesterol proportion of Phytosterols Seed SdCampesterol_Cp Campesterol CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. End of flowering (BBCH 69) date – Estimation kg day-of-year branched Alcyl-chain content SdBranched Pod and stem Pod and stem branched Alcyl-chain content The concentration of all compounds with branched alcyl chains from pod and stem material Calcium content LfCa Leaf Calcium content The concentration of Calcium in leaves Leaf The concentration of Campesterol in seeds Seed Campesterol content Campesterol content SdCampesterol Campesterol Seed pod/20cm of terminal raceme Crop problems annotations - Estimation Visual observations SdStearic Stearic acid content Seed stearic acid content Seed The concentration of stearic acid in seeds. Seed linolenic acid content The concentration of linolenic acid in seeds. Seed StLinolenic Linolenic acid content SdPhytosterol Seed total Phytosterol content Seed The total concentration of phytosterols in seeds Phytosterol content seed Moisture content SdMoist Seed Seed moisture content. Seed moisture content Surface is determined by the experimental design Plot Size – Computation Valid Assessment on measure validity. Plot Plot measure validity Measure validity Leaf The concentration of Copper in leaves LfCu Copper content Leaf Copper content LfPb Leaf Lead content The concentration of Lead in leaves Leaf Lead content beak length Beak length Pod beak length LLS incidence (diseased) - Estimation 10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%. Leaf Molybdenum content LfMo Leaf Molybdenum content The concentration of Molybdenum in leaves μmol/g Pod and stemprimary Alkohol functional group content SdAlcI The concentration of components with primary alcohol functional groups from pod and stem material Pod and stem primary Alkohol functional group content LfSe Leaf Selenium content The concentration of Selenium in leaves Leaf Selenium content Additional stages for pod maturity according to the BBCH scale (82-99) Development stage Plant pod maturity stage;time to BBCH growth stage 82-99 Pod maturity time (BBCH 82-99) Seed moisture content humidifier - Measurement Humidimeter (sample of seeds) Leaf miner incidence (diseased) - Counting 10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner. Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging Plant lodging incidence - Estimation Pedicel length Pedicel length of pods from terminal raceme length pedicel SdMg The concentration of Magnesium in seeds Seed Magnesium content Seed Magnesium content NIRS assay. Pct total fatty acids Linoleic seed content NIRS - Measurement Ketone functional group content SdKeto The concentration of components with ketone functional groups from pod and stem material Pod and stem Pod and stem Ketone functional group content NIRS assay. Pct total fatty acids Palmitic seed content NIRS - Measurement Seed SdSe Selenium content Seed Selenium content The concentration of Selenium in seeds Stem Light leaf spot severity on stems, judged by the percentage of area covered by LLS. LLS severity on stem (percentage of stem area covered by LLS) Light leaf spot severity 0-7 percentage scale Plant stand at emergence Percentage of seeds that emerge. Seed Plant emergence PltSdEm Seed palmitic acid content Seed Palmitic acid content SdPalmitic The concentration of palmitic acid in seeds. Phoma leaf spot incidence (diseased) - Estimation 10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pedicel length - Measurement variable_of NIRS assay. Pct total fatty acids Linolenic seed content NIRS - Measurement Leaf Phosphorus content Phosphorus content LfP Leaf The concentration of Phosphorus in leaves Pod and stem Aldehyde functional group content SdAld Pod and stem The concentration of components with aldehyde functional groups from pod and stem material Aldehyde functional group content Seed Sigmasterol content Stigmasterol content Seed SdStigmasterol Stigmasterol The concentration of Stigmasterol in seeds Additionnal pod maturity stages (BBCH 82-99) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS. Pod Light leaf spot severity LLS severity on pods (percentage of pod area covered by LLS) 0-9 Lodging Incidence scale Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter. Seed sieving - Estimation NIRS assay. Pct of Seed Dry Weight Protein seed content NIRS - Measurement SdPb Lead content Seed The concentration of Lead in seeds Seed Lead content Seed Strontium content Seed SdSr The concentration of Strontium in seeds Strontium content Protein content SdProt The concentration of proteins in seeds. Seed protein content Seed 0-9 Damage/Incidence scale scale_of method_of Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Pod Number - Counting Cycloartenol Cycloartenol content Seed SdCycloartenol Seed Cycloartenol content The concentration of Cycloartenol in seeds Score the damage caused by hail. HailDmg - Estimation Seed impurity content – Computation Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively Plant Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale) First inter-node time (BBCH 31) Development stage BBCH growth stage 31;CETIUM growth stage C1 plants/6 plants Seed Erucid acid content Seed erucic acid content The concentration of erucic acid in seeds. SdErucic Early pod development (BBCH 73) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded. Verticilium wilt presence - Counting Leaf The concentration of Sodium in leaves LfNa Sodium content Leaf Sodium content Plot measure validity – Estimation Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid” The concentration of Phosphorus in seeds Seed Seed Phosphorus content dSP Phosphorus content Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. No of plants with Sclerotinia (out of six) Plant Sclerotinia incidence Pod and stem Fatty acids functional group content Fatty acids functional group content The concentration of components with fatty acids as functional groups from pod and stem material SdFA Pod and stem 0-3 disease stage scale CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of flowering (BBCH 61) date - Estimation Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate). Sclerotinia incidence (diseased) - Counting LLS resistance - Estimation 10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7. 10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot. Phoma incidence (diseased) - Counting Leaf Strontium content Leaf Strontium content The concentration of Strontium in leaves LfSr Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O) Oil yield - Computation Seed weight - Measurement Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. 10 spots per plant]]> Leaf phoma resistance - Estimation Pod length - Measurement Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod development stages (BBCH 72-79) date – Estimation root Oleic seed content NIRS - Measurement NIRS assay. Pct total fatty acids mg Plant stand - Counting Count the number of plants on linear sections defined in each sowing line at the exception of the border lines mm