SdZn_M_mgkg Nottingham Thomas Alcock, David Salt erucicSeedCont_M_pct INRA Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC Anne Laperche Nottingham Martin Broadley, Catherine Thomas LRN_Ct_root INRA cropAnnot_E_cmt Anne Laperche Crop problems;Crop problems during the cycle.;CROP Martin Broadley, Catherine Thomas SD_Est_mm Nottingham Yongju Huang, John West University of Hertfordshire VertWilt_Cou_pct Nottingham Martin Broadley,Thomas Alcock LfSe_M_mgkg SdAvenasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted 2-25% girdling 2 Richard Broughton, Frederic Beaudoin Rothamsted SdCycloartenol_Cp_pct INRA phomaDmg_CompG2_0to9 Anne Laperche Phoma damage G2 score LLSleaf_E_pct Yongju Huang, John West University of Hertfordshire LfCd_M_mgkg Nottingham Martin Broadley,Thomas Alcock Nottingham SdS_M_mgkg Thomas Alcock, David Salt <5 spots per plant 1 Thomas Alcock, David Salt SdPb_M_mgkg Nottingham SdAlcII_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR INRA Anne Laperche seedImpur_M_pct Hail Damage;HAILDMG hailDmg_E_0to9 INRA Anne Laperche 0 NA INRA Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY Anne Laperche stagePodMaturity_cnt_dayj Nottingham LfNi_M_mgkg Martin Broadley,Thomas Alcock severe damage 9 Rothamsted SdFA_M_mgkg Richard Broughton, Frederic Beaudoin Anne Laperche linoleicSeedCont_M_pct Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC INRA LfZn_M_mgkg Nottingham Martin Broadley,Thomas Alcock INRA Anne Laperche seedMoist_mnirs_pct Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS LfSr_M_mgkg Nottingham Martin Broadley,Thomas Alcock PhomaLS_Cou_plnt10plnt University of Hertfordshire Yongju Huang, John West plntStdEmerg_M_plt INRA Anne Laperche Plant Emergence;Percentage of seeds that emerge;EMERG Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC INRA linolenicSeedCont_M_pct Anne Laperche Thomas Alcock, David Salt SdFe_M_mgkg Nottingham Nottingham SdSe_M_mgkg Thomas Alcock, David Salt Nottingham LRL_M_cm Martin Broadley, Catherine Thomas stageG5_cnt_dayj seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5 INRA Growth Stage G5;10% of the seeds have reached maturity Anne Laperche Nottingham Martin Broadley,Thomas Alcock LfAl_M_mgkg Plant Size;Height of plants in an experimental plot;PLANTSIZE INRA Anne Laperche plntHeight_M_cm LfMg_M_mgkg Nottingham Martin Broadley,Thomas Alcock Nottingham Martin Broadley,Thomas Alcock LfP_M_mgkg INRA Anne Laperche NHI_Comp_pct Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI seedMoistHarv_mhumi_pct INRA Anne Laperche Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV SdP_M_mgkg Nottingham Thomas Alcock, David Salt Thomas Alcock, David Salt Nottingham SdMo_M_mgkg FtLen_M_cm John Innes Centre Rachel Wells Sclerot_Cou_plnt6plnt Yongju Huang, John West University of Hertfordshire white blight 100% girdling ( stem weak/firm) 6 corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1 Anne Laperche Growth Stage F1;First flowers are open stageF1_cnt_dayj INRA Nottingham Martin Broadley,Thomas Alcock LfCu_M_mgkg Growth Stage F2;10% of the flowers are open on the main inflorescence elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2 INRA Anne Laperche stageF2_cnt_dayj LRD_Cp_rootcm Martin Broadley, Catherine Thomas Nottingham Nottingham Martin Broadley,Thomas Alcock LfCs_M_mgkg weevilsDmg_E_0to9 INRA Anne Laperche Weevils (all species) damage score;charancon 0 No leaf spotting Thomas Alcock, David Salt SdB_M_mgkg Nottingham LLSleaf_E_0to7 Yongju Huang, John West University of Hertfordshire LfMn_M_mgkg Nottingham Martin Broadley,Thomas Alcock Nottingham SdMg_M_mgkg Thomas Alcock, David Salt 7 regular SdCampesterolDiunsat_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Nottingham Martin Broadley,Thomas Alcock LfB_M_mgkg Nottingham Martin Broadley,Thomas Alcock LfK_M_mgkg Anne Laperche plotHarvest_M_kg INRA Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST SdSr_M_mgkg Thomas Alcock, David Salt Nottingham SdBranched_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Rothamsted SdB-Sitosterol_M_mgkg Richard Broughton, Frederic Beaudoin John Innes Centre Rachel Wells FertBraNumb_Ct_Fb StemWid_M_cm John Innes Centre Rachel Wells SdBrassicasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Nottingham TRL_Cp_cm Martin Broadley, Catherine Thomas Yongju Huang, John West University of Hertfordshire white blight Sclerot_Cou_plntPlot Nottingham Martin Broadley,Thomas Alcock LfTi_M_mgkg Richard Broughton, Frederic Beaudoin SdAlcI_M_mgkg Rothamsted Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO INRA Anne Laperche stageFlo_cnt_dayj PowMildew_E_0or1 Yongju Huang, John West University of Hertfordshire Rachel Wells SdWght_M_mg John Innes Centre Nottingham Martin Broadley,Thomas Alcock LfFe_M_mgkg Martin Broadley,Thomas Alcock LfS_M_mgkg Nottingham Nottingham MLRL_Cp_cm Martin Broadley, Catherine Thomas Oil Yield;Oil yield per ha;OY oilYield_Comp_qha INRA Anne Laperche SdCr_M_mgkg Nottingham Thomas Alcock, David Salt flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4 INRA stageG4_cnt_dayj Growth Stage G4;30% of the pods have reached their final size Anne Laperche Yongju Huang, John West University of Hertfordshire PhomaLS_E_0to3 high > 90% 1 Nottingham Thomas Alcock, David Salt SdAs_M_mgkg Richard Broughton, Frederic Beaudoin SdKeto_M_mgkg Rothamsted glsSeedCont_M_μmolg Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS INRA Anne Laperche Anne Laperche plotSize_M_m2 INRA Plot Surface;Surface of the elementary experimental plot;PLOTSURF 4 Diseased leaved with a few large lesions of many small leasions, area quite heavily infected Rachel Wells PodNumbTermRac_Ct_PodTermRac John Innes Centre Anne Laperche Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC INRA climaticAnnot_E_cmt 1 trace Nottingham Martin Broadley,Thomas Alcock LfNa_M_mgkg high irregular 1 Anne Laperche stagePodDev_cnt_dayj Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV INRA Nottingham Martin Broadley,Thomas Alcock LfBa_M_mgkg SdCycloartenol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted John Innes Centre PodLen_M_cm Rachel Wells INRA Anne Laperche podShat_E_score Pod shattering;Scoring of pods open just before harvest;PODSHAT flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1 stageG1_cnt_dayj INRA Growth Stage G1;10% of the pods have reached their final size Anne Laperche Nottingham Martin Broadley,Thomas Alcock LfU_M_mgkg Yongju Huang, John West University of Hertfordshire VertWilt_E_0or1 SdMn_M_mgkg Nottingham Thomas Alcock, David Salt Nottingham Thomas Alcock, David Salt SdCd_M_mgkg Anne Laperche corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO INRA stageEndFlo_cnt_dayj Growth Stage End Flowering;End of flowering on the main inflorescence Nottingham LfRb_M_mgkg Martin Broadley,Thomas Alcock PodWid_M_cm John Innes Centre Rachel Wells Martin Broadley,Thomas Alcock LfCa_M_mgkg Nottingham low damage 3 1 ≤ 5% girdling LfAg_M_mgkg Nottingham Martin Broadley,Thomas Alcock 26-50% girdling 3 LLSpods_E_pct University of Hertfordshire Yongju Huang, John West Rothamsted Richard Broughton, Frederic Beaudoin SdCampesterol_Cp_pct Anne Laperche Plant Lodging;Scoring of the plant lodging of the plot;LODGING lodging_E_0to9 INRA Sdd-toc_M_mgkg Swen Langer, Lenka Havlickova University of York INRA validateannot_E_cmt Anne Laperche Annotations on measure validity;VALIDATION Richard Broughton, Frederic Beaudoin SdPhytosterol_M_mgkg Rothamsted Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL INRA Anne Laperche protMealCont_M_pct >10 spots per plant 3 INRA seedMoist_mlab_pct Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB Anne Laperche Anne Laperche stageC1_cnt_dayj corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1 Growth Stage C1;Bolting date when the first inter-node appears INRA Half green/half diseased, leaf curling and distortion 5 PhomaLS_Cou_pct Yongju Huang, John West University of Hertfordshire Martin Broadley,Thomas Alcock Nottingham LfAs_M_mgkg INRA Biotic Stress;Biotic attack during the crop cycle;BIOTIC Anne Laperche bioticAnnot_an_text Yongju Huang, John West University of Hertfordshire LLSstem_E_pct Rachel Wells SdNum_Ct_sd John Innes Centre 7 high damage SdTotalWax_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Nottingham SdCu_M_mgkg Thomas Alcock, David Salt high to severe damage 8 Anne Laperche Oil %;Content of oil in seeds determined by NIRS;O INRA oilSeedCont_M_pct Nottingham SdK_M_mgkg Thomas Alcock, David Salt SdB-Sitosterol_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted Nottingham SdNa_M_mgkg Thomas Alcock, David Salt Yongju Huang, John West LeafMiner_Cou_pct University of Hertfordshire Yongju Huang, John West University of Hertfordshire PhomaSKupper_E_0to7 4 51-75% girdling 0 NA Nottingham Martin Broadley,Thomas Alcock LfCr_M_mgkg Swen Langer, Lenka Havlickova University of York Sda-toc_M_mgkg INRA regStand_E_0to9 Anne Laperche Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND SdCampesterol diunsat_Cp_pct Rothamsted Richard Broughton, Frederic Beaudoin NA 0 < 10% 9 7 100% girdling (stem dead/lodged)) entity Prot Yield;Protein yield per ha;PrY protYield_Comp_qha INRA Anne Laperche PhomaSK_E_0to7 Yongju Huang, John West University of Hertfordshire 60-90% 3 Richard Broughton, Frederic Beaudoin SdAld_M_mgkg Rothamsted LfMo_M_mgkg Nottingham Martin Broadley,Thomas Alcock 5-10 spots per plant 2 Richard Broughton, Frederic Beaudoin SdAvenasterol_Cp_pct Rothamsted Rachel Wells PltHght_M_cm John Innes Centre SdStigmasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Sdy-toc_M_mgkg Swen Langer, Lenka Havlickova University of York Plant Density;Number of viable plants counted per surface unit;PLANTDENS INRA Anne Laperche plntStnd_M_plntm2 low to intermediate damage 4 0 no symptoms observable INRA Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC Anne Laperche stearicSeedCont_M_pct 7 10-30% BeakLen_M_cm Rachel Wells John Innes Centre Brassica napus ontology Brassica ontology Yongju Huang, John West University of Hertfordshire PhomaSKbase_E_0to7 Richard Broughton, Frederic Beaudoin SdStigmasterol_Cp_pct Rothamsted Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC oleicSeedCont_M_pct INRA Anne Laperche Thomas Alcock, David Salt SdRb_M_mgkg Nottingham Nottingham Martin Broadley,Thomas Alcock LfPb_M_mgkg Culture leaning, without impact 2 Nottingham Martin Broadley, Catherine Thomas PRL_M_cm SdCo_M_mgkg Thomas Alcock, David Salt Nottingham 4 8 4 SdCampesterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Thomas Alcock, David Salt SdLi_M_mgkg Nottingham palmiticSeedCont_M_pct INRA Anne Laperche Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC 6 Nottingham Martin Broadley,Thomas Alcock LfV_M_mgkg Plants heavily stunted or dead. 7 Diseased leaves with non or a few small lesions 2 PedLen_M_cm Rachel Wells John Innes Centre acronym SNi_M_mgkg Thomas Alcock, David Salt Nottingham Plants with scattered lesions 3 intermediate to high damage 6 Thomas Alcock, David Salt SdCa_M_mgkg Nottingham 5 75-100% girdling irregular 3 perfectly regular 9 1 Trace of disease 5 30-60% 3 Richard Broughton, Frederic Beaudoin SdAlk_M_mgkg Rothamsted 5 intermediate damage Evidence of stunting 6 attribute 2 0 No disease observable Protein %;Content of proteins in seeds determined by NIRS;Pr protSeedCont_M_pct INRA Anne Laperche 2 very low damage SdBrassicasterol_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted 0 no visible affection 5 moderately regular Average loding 5 1 No lodging Development stage Additional flowering stages according to the BBCH scale (62-65) Flowering time (BBCH 62-65) Plant flowering stage;time to BBCH growth stage 62-65;Anthesis CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. End of flowering (BBCH 69) date – Estimation The concentration of components with aldehyde functional groups from pod and stem material SdAld Pod and stem Aldehyde functional group content Pod and stem Aldehyde functional group content Visual observations Climatic incidents annotions - Estimation Seed sieving - Estimation Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter. % plants/plot SdPhytosterol The total concentration of phytosterols in seeds Phytosterol content Seed total Phytosterol content Seed mg SdPalmitic The concentration of palmitic acid in seeds. Seed Seed palmitic acid content Palmitic acid content Linolenic acid content Seed linolenic acid content Seed The concentration of linolenic acid in seeds. StLinolenic Light leaf spot severity Light leaf spot severity on stems, judged by the percentage of area covered by LLS. LLS severity on stem (percentage of stem area covered by LLS) Stem Crop problems annotation Plant Crop problems during the crop cycle. Exposure to crop problems;damage from crop problem CropPb Seed moisture content. Seed SdMoist Seed moisture content Moisture content alpha-tocopherol content Seed alpha- tocopherol content Sda-toc Alpha- tocopherol content in seeds seed Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation LfZn Leaf Zinc content The concentration of Zinc in leaves Zinc content Leaf Incidence of Phoma leaf spot Phoma incidence The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot Leaf The concentration of Avenasterol in seeds SdAvenasterol Avenasterol Seed Seed Avenasterol content Avenasterol content Protein content The concentration of proteins in the meal. Meal protein content Meal MlProt Plant stand - Counting Count the number of plants on linear sections defined in each sowing line at the exception of the border lines Seed Oleic acid content Seed oleic acid content The concentration of oleic acid in seeds. StOleic Leaf Leaf Silver content Silver content LfAg The concentration of Silver in leaves Plant lodging incidence - Estimation Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging The plant lodging incidence. Lodging incidence Plant lodging incidence Lodg Plant Number of pods on terminal raceme Pod number number pod A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester plotharvest - Measurement Oil yield Plant Oil yield Oil yield OY NIRS assay Seed moisture NIRS - Measurement The concentration of Caesium in leaves Leaf Caesium content LfCs Leaf Caesium content Molybdenum content LfMo Leaf Molybdenum content Leaf The concentration of Molybdenum in leaves LLS severity on pods (percentage of pod area covered by LLS) Pod Light leaf spot severity Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS. First flowers (BBCH 60) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Oil content Seed SdOil Seed oil content The concentration of oil in seeds. 0-9 Lodging Incidence scale First inter-node (BBCH 31) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. The concentration of Manganese in seeds SdMn Seed Manganese content Seed Manganese content The concentration of Potassium in leaves Leaf LfK Potassium content Leaf Potassium content Seed SdMoist Seed moisture content Moisture content Seed moisture content. Seed impurity content – Computation Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively mg/kg Pedicel length Pedicel length of pods from terminal raceme pedicel length NIRS assay. Pct total fatty acids Linolenic seed content NIRS - Measurement HailDmg - Estimation Score the damage caused by hail. Size Surface of the elementary experimental plot Plot PlotSize Plot size The concentration of Arsenic in leaves Leaf LfAs Arsenic content Leaf Arsenic content Lateral root length length Lateral root Length of the lateral root LRL SdProt Seed Seed protein content Protein content The concentration of proteins in seeds. Manganese content Leaf The concentration of Manganese in leaves LfMn Leaf Manganese content Seed SdMo Seed Molybdenum content Molybdenum content The concentration of Molybdenum in seeds kg GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement * Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation. * Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step. * 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples. * Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour. * Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials. * A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions. * The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes. * 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used). * The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane. * Samples are vortexed, centrifuged and the upper hexane phase is removed. * A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first. * Samples are dried in a rotary evaporator or under a stream of nitrogen. * Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID). *GC program: * Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes. * Inlet temp: 325°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C Pod and stem SdAlcII secondary Alkohol functional group content The concentration of components with secondary alcohol functional groups from pod and stem material Pod and stem secondary Alkohol functional group content Ketone functional group content The concentration of components with ketone functional groups from pod and stem material SdKeto Pod and stem Ketone functional group content Pod and stem Length of lower stem from ground level to last formed fertile branch Foot length foot length Seed Zinc content Zinc content The concentration of Zinc in seeds SdZn Seed Seed diunsaturated Campesterol content Campesterol diunsat proportion of Phytosterols The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds Campesterol diunsat SdCampesterolDiunsat_Cp Seed Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant width stem Stem width The concentration of Strontium in leaves Strontium content LfSr Leaf Leaf Strontium content Seed impurity content Impurities content SdImpur Percentage of impurities in a sample of seeds determined by weighing. Seed 3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg Seed mineral analysis - Measurement Verticilium wilt presence - Counting Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded. Plot measure validity Plot Measure validity Valid Assessment on measure validity. Sclerotinia incidence (diseased) - Counting The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded. Phoma stem canker upper stem lesion score Upper stem Phoma stem cancer severity Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Lateral root number LRN number Lateral root Number of lateral roots The percentage of plants that showed presence of light leaf spot incidence on leaves Light leaf spot incidence LLS incidence on leaf Leaf method_of Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Plant height - Measurement Crop problems annotations - Estimation Visual observations SdSe Selenium content The concentration of Selenium in seeds Seed Selenium content Seed SdRb Seed Rubidium content Seed The concentration of Rubidium in seeds Rubidium content 30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale) Plant Development stage Early pod development time (BBCH 73) BBCH growth stage 73;CETIUM growth stage G4 NIRS assay. Pct total fatty acids Linoleic seed content NIRS - Measurement Stigmasterol The concentration of Stigmasterol in seeds Stigmasterol content SdStigmasterol Seed Seed Sigmasterol content Seed Phosphorus content dSP Phosphorus content Seed The concentration of Phosphorus in seeds Withering disease Withering disease caused by the agent Leptosphaeria maculans (Phoma). PhomaDmg Plant Phoma damage Phoma damage 0-3 disease stage scale Avenasterol The percentage of Avenasterol in the total of Phytosterols in seeds Seed Avenasterol proportion of Phytosterols Seed Avenasterol content SdAvenasterol_Cp Seed Magnesium content SdMg Magnesium content Seed The concentration of Magnesium in seeds Image analysis - Counting RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN). Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O) Oil yield - Computation Leaf mineral analysis - Measurement 3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry Leaf Nickel content LfNi Leaf Nickel content The concentration of Nickel in leaves Seed Iron content Iron content Seed The concentration of Iron in seeds SdFe Plant Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. No of plants with Sclerotinia (out of six) Sclerotinia incidence 10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded Phoma leaf spot incidence (diseased) - Estimation Leaf Incidence with leaf miner Leaf miner incidence The percentage of plants that showed presence of leaf miner from a subsample of a plot Humidimeter (sample of seeds) Seed moisture content humidifier - Measurement height PH plant Plant height Maximum plant height from ground to tip of terminal raceme SD The diameter of a seed Seed diameter Seed diameter cm BBCH growth stage 69;end flowering stage Plant End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale) End of flowering time (BBCH 69) Development stage Length of the primary root PRL length Primary root Primary root lengh Pod Number - Counting Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. SdSr Seed Seed Strontium content The concentration of Strontium in seeds Strontium content Leaf LfNa The concentration of Sodium in leaves Sodium content Leaf Sodium content 0-7 percentage scale pod development stage;time to BBCH growth stages 72-79 Development stage Pod development time (BBCH 72-79) Plant Additional stages for pod development according to the BBCH scale (72-79) Lateral root average length MLRL Mean lateral root length The mean length of a lateral root of that root system SdFA Pod and stem Fatty acids functional group content The concentration of components with fatty acids as functional groups from pod and stem material Fatty acids functional group content Pod and stem Verticilium wilt presence Verticilium wilt presence Absence or presence of Verticilium wilt on the plant Plant 0-9 Regularity scale Titanium content Leaf The concentration of Titanium in leaves LfTi Leaf Titanium content Seed Cadmium content SdCd Seed The concentration of Cadmium in seeds Cadmium content Stem length - Measurement Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. LfAl The concentration of Aluminium in leaves Aluminium content Leaf Leaf Aluminium content Seed Campesterol content Campesterol Seed SdCampesterol_Cp Campesterol proportion of Phytosterols The percentage of Campesterol in the total of Phytosterols in seeds Visual observations Biotic stress annotations - Estimation Leaf miner incidence (diseased) - Counting 10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner. Brassicasterol The percentage of Brassicasterol in the total of Phytosterols in seeds Brassicasterol proportion of Phytosterols Seed Seed Brassicasterol content SdBrassicasterol Biotic attack during the crop cycle. BioticPb Plant Exposure to biotic attack; damage du to biotic attack Biotic attack annotation HPLC (High performance liquid chromatography) analysis - Measurement A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents Pod shattering Pod Shattering PodShat Proportion of open pods (assessment) SdCampesterol Campesterol content Seed Campesterol content Campesterol The concentration of Campesterol in seeds Seed SdCr Seed The concentration of Chromium in seeds Seed Chromium content Chromium content Additionnal pod development stages (BBCH 72-79) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Seed Number - Counting Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Powdery mildew presence - Estimation Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1 Comment seed Seed gamma- tocopherol content Gamma- tocopherol conten in seeds gamma-tocopherol content Sdy-toc NHI Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root) Seed N content to total plant N content ratio Nitrogen Harvest Index N content proportion of plant N content Seed Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Fertile Branches - Counting The concentration of Lithium in seeds Seed SdLi Lithium content Seed Lithium content Cycloartenol content SdCycloartenol Cycloartenol The concentration of Cycloartenol in seeds Seed Cycloartenol content Seed The concentration of stearic acid in seeds. Stearic acid content Seed stearic acid content SdStearic Seed LfSe Leaf Selenium content The concentration of Selenium in leaves Leaf Selenium content 10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot. Phoma incidence (diseased) - Counting Number of plants with phoma leaf spots Phoma leaf spots The number of plants that showed presence of phoma leaf spots from a subsample of a plot Plant Development stage Plant Beginning of pod development time (BBCH 71) BBCH growth stage 71;CETIUM growth stage G1 10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale) Seed SdStigmasterol_Cp Stigmasterol proportion of Phytosterols Seed Sigmasterol content Stigmasterol The percentage of Stigmasterol in the total of Phytosterols in seeds Palmitic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Vanadium content LfV The concentration of Vanadium in leaves Leaf Leaf Vanadium content Plant Exposure to climatic stress Climatic incidents during the crop cycle. Climatic incidents annotation ClimPb Leaf Copper content Leaf Copper content The concentration of Copper in leaves LfCu Seed The concentration of Calcium in seeds Calcium content SdCa Seed Calcium content variable_of Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image. Stem witdh - Measurement Plant Development stage Beginning of pod maturity time (BBCH 81) 10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale) BBCH growth stage 81;CETIUM growth stage G5 Leaf The concentration of Cadmium in leaves Cadmium content LfCd Leaf Cadmium content Plant damage caused by hail. HailDmg Hail damage Plant Hail damage Pod and stemprimary Alkohol functional group content The concentration of components with primary alcohol functional groups from pod and stem material SdAlcI primary Alkohol functional group content Pod and stem Seed glucosinolate content SdGLS Seed Glucosinolate content The concentration of glucosinolates in seeds. Leaf The concentration of Magnesium in leaves Magnesium content Leaf Magnesium content LfMg Leaf phoma resistance - Estimation 10 spots per plant]]> Nitrate Harvest index - Computation Samples from HI assay are analysed for N_%DW in the different fractions Cycloartenol proportion of Phytosterols The percentage of Cycloartenol in the total of Phytosterols in seeds Seed Cycloartenol Seed Cycloartenol content SdCycloartenol_Cp Plant stand at emergence – Computation EMERG = (Number of plants obtained / Number of seeds sown) x 100 Seed Nickel content Seed Nickel content SdNi The concentration of Nickel in seeds Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid” Plot measure validity – Estimation Delta- tocopherol content in seeds Seed delta- tocopherol content seed delta-tocopherol content Sdd-toc seed The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Length of all roots Total root length length TRL Total root Weevils damage WeevilsDmg Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil). Weevils damage Plant Pod and stem The concentration of wax in the pod and seed material wax content Pod and stem wax content SdTotalWax Pod length Pod length of pods from terminal raceme length pod Leaf Leaf Iron content Iron content The concentration of Iron in leaves LfFe Seed Brassicasterol content Seed Brassicasterol content The concentration of Brassicasterol in seeds SdBrassicasterol Brassicasterol Seed Seed B-Sitosterol content B-Sitosterol The concentration of B-Sitosterol in seeds B-Sitosterol content SdB-Sitosterol GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement * 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.). * Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers. * Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C. * Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. * After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS. * GC program: * Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes. * Inlet temp: 280°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) NIRS assay. Pct total fatty acids Stearic seed content NIRS - Measurement Absence or presence of powdery mildew Presence (1) or absence (0) of powdery mildew Plant Powdery mildew presence Evaluation of the Phoma lingam infection with the G2 score. The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation. Phoma damage – G2 score – Computation 2020-10-19T00:00:02 day-of-year q/ha Pod and stem Alkane-functional group content Alkane-functional group content Pod and stem SdAlk The concentration of components with alkane functional groups from pod and stem material 0-9 Damage/Incidence scale CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Addictional flowering stages (BBCH 62-65) date – Estimation Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation Phoma stem cancer severity Stem Phoma stem canker severity Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod maturity stages (BBCH 82-99) date – Estimation The concentration of Phosphorus in leaves Leaf Phosphorus content Leaf LfP Phosphorus content Beginning of pod maturity (BBCH 81) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. The concentration of diunsaturated Campesterol in seeds Seed diunsaturated Campesterol content Seed diunsaturated Campesterol content SdCampesterolDiunsat Campesterol diunsat LLS resistance - Estimation 10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7. Protein yield Seed protein yield Protein yield per ha Seed PrY Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant. Pod beak length - Measurement Light leaf spot resistance Light Leaf spot severity on leafs Leaf LLS severity on leaf scale_of Leaf Leaf phoma resistance Severity assessment of phoma leaf spot Phoma leaf spot severity Leaf Rubidium content Leaf LfRb The concentration of Rubidium in leaves Rubidium content Oil seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight Seed moisture content – Computation Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively root/cm Scoring of presence or absence of verticilium wilt symptoms across whole plant. Verticilium wilt presence - Estimation Number of fertile branches Number of fertile branches number fertile branch Beak length length beak Pod beak length Biomass Yield Biomass yield Weight (kg) of raw materials harvested by plot Leaf Calcium content Leaf LfCa Calcium content The concentration of Calcium in leaves Protein meal content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight Plant height - Measurement Height is measured from ground to the top of the plants using a dipstick graduated in cm fertile branch Seed linoleic acid content Seed Linoleic acid content The concentration of linoleic acid in seeds. StLinoleic The concentration of Copper in seeds Copper content Seed Copper content Seed SdCu LfU Leaf Uranium content Leaf Uranium content The concentration of Uranium in leaves The concentration of Cobalt in seeds SdCo Seed Cobalt content Seed Cobalt content Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate). Sclerotinia incidence (diseased) - Counting Pod shattering - Estimation Visual notations (scale 0 - 9) Leaf Chromium content Leaf The concentration of Chromium in leaves Chromium content LfCr SdS Seed Seed Sulphur content The concentration of Sulphur in seeds Sulphur content No of plants with Sclerotinia (per plot) Plant Sclerotinia incidence Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. LLS incidence (diseased) - Estimation 10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%. 10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated. LLS severity - Estimation pod/20cm of terminal raceme First inter-node time (BBCH 31) Plant Development stage BBCH growth stage 31;CETIUM growth stage C1 Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale) Leaf LfB Leaf Boron content Boron content The concentration of Boron in leaves Erucid seed content NIRS - Measurement NIRS assay. Pct total fatty acids SdK The concentration of Potassium in seeds Seed Seed Potassium content Potassium content plant/m2 GLS seed content NIRS - Measurement NIRS assay Quantity per Seed Dry Weight Seed Lead content Seed SdPb Lead content The concentration of Lead in seeds width pod Pod width Pod width of pods from terminal raceme CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of pod development (BBCH 71) date – Estimation The concentration of Lead in leaves Lead content Leaf LfPb Leaf Lead content Plant stand Plant density Crop stand Number of viable plants counted per surface unit Plant Stand PltSd Verticillium wilt incidence Verticilium wilt presence The percentage of plants that showed presence of leaf miner from a subsample of a plot Plant The concentration of all compounds with branched alcyl chains from pod and stem material SdBranched branched Alcyl-chain content Pod and stem branched Alcyl-chain content Pod and stem Sodium content Seed The concentration of Sodium in seeds SdNa Seed Sodium content Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod length - Measurement regularity stand - Estimation Visual notations (scale 0 - 9) Weevil damage - Estimation Observe damage caused by Weevils (all species), and rate the damage. seed Seed number number Number of seeds in ten typical pods SdB-Sitosterol_Cp Seed B-Sitosterol content The percentage of B-Sitosterol in the total of Phytosterols in seeds B-Sitosterol Seed B-Sitosterol proportion of Phytosterols Relative seed Phytosterol content - Computation The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols LLS severity - Estimation 10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated. 0-7 disease stage scale Pedicel length - Measurement Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Image analysis - Computation The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001) boolean BBCH growth stage 61;CETIUM growth stage F2 Development stage 10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale) Beginning of flowering time (BBCH 61) Plant Balium content The concentration of Balium in leaves LfBa Leaf Leaf Balium content The concentration of Arsenic in seeds Seed Seed Arsenic content SdAs Arsenic content Regularity of plant stand Scoring of the regularity of the plant stand Stand Plant mm Development stage First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale) BBCH growth stage 60;CETIUM growth stage F1 First flowers time (BBCH 60) Plant plants/10 plants Plant pod maturity stage;time to BBCH growth stage 82-99 Pod maturity time (BBCH 82-99) Development stage Additional stages for pod maturity according to the BBCH scale (82-99) Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr) Seed protein Yield - Computation Beginning of flowering (BBCH 61) date - Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. NIRS assay. Pct total fatty acids Oleic seed content NIRS - Measurement LfS The concentration of Sulphur in leaves Leaf Sulphur content Leaf Sulphur content 0-5 percentage bracket scale Phoma stem canker base stem score Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity Stem base Surface is determined by the experimental design Plot Size – Computation Erucid acid content Seed erucic acid content SdErucic The concentration of erucic acid in seeds. Seed Seed Percentage of seeds that emerge. Plant stand at emergence Plant emergence PltSdEm root Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod width - Measurement The concentration of Boron in seeds Seed Boron content Seed SdB Boron content Protein seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight plants/6 plants Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Seed weight - Measurement weight Seed weight seed Seed weight of ten typical pods Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Early pod development (BBCH 73) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. The mean density of lateral roots along the primary root LRD density Lateral root density Lateral root μmol/g