Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV Anne Laperche INRA seedMoistHarv_mhumi_pct 1 No lodging TRL_Cp_cm Nottingham Martin Broadley, Catherine Thomas Rothamsted SdBrassicasterol_M_mgkg Richard Broughton, Frederic Beaudoin Richard Broughton, Frederic Beaudoin SdCampesterol_Cp_pct Rothamsted SdRb_M_mgkg Thomas Alcock, David Salt Nottingham white blight University of Hertfordshire Yongju Huang, John West Sclerot_Cou_plntPlot SdMo_M_mgkg Nottingham Thomas Alcock, David Salt LfCr_M_mgkg Martin Broadley,Thomas Alcock Nottingham Rothamsted SdCycloartenol_Cp_pct Richard Broughton, Frederic Beaudoin Richard Broughton, Frederic Beaudoin SdBrassicasterol_Cp_pct Rothamsted SdNa_M_mgkg Nottingham Thomas Alcock, David Salt Anne Laperche validateannot_E_cmt INRA Annotations on measure validity;VALIDATION INRA Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC linoleicSeedCont_M_pct Anne Laperche University of York Swen Langer, Lenka Havlickova Sdd-toc_M_mgkg University of Hertfordshire Yongju Huang, John West PowMildew_E_0or1 2 2-25% girdling INRA Anne Laperche palmiticSeedCont_M_pct Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC Nottingham LfNa_M_mgkg Martin Broadley,Thomas Alcock Martin Broadley,Thomas Alcock Nottingham LfZn_M_mgkg Growth Stage F1;First flowers are open corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1 stageF1_cnt_dayj Anne Laperche INRA Nottingham Martin Broadley, Catherine Thomas LRD_Cp_rootcm Average loding 5 Rothamsted SdB-Sitosterol_Cp_pct Richard Broughton, Frederic Beaudoin oilSeedCont_M_pct INRA Anne Laperche Oil %;Content of oil in seeds determined by NIRS;O SdAvenasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdSe_M_mgkg Nottingham Thomas Alcock, David Salt Anne Laperche Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI INRA NHI_Comp_pct 30-60% 5 INRA Anne Laperche seedImpur_M_pct Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR Anne Laperche Biotic Stress;Biotic attack during the crop cycle;BIOTIC bioticAnnot_an_text INRA 0 No disease observable Anne Laperche Oil Yield;Oil yield per ha;OY oilYield_Comp_qha INRA Thomas Alcock, David Salt Nottingham SdK_M_mgkg John Innes Centre Rachel Wells StemWid_M_cm Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV Anne Laperche INRA stagePodDev_cnt_dayj Growth Stage End Flowering;End of flowering on the main inflorescence stageEndFlo_cnt_dayj corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO INRA Anne Laperche Nottingham Thomas Alcock, David Salt SdMn_M_mgkg LfAg_M_mgkg Martin Broadley,Thomas Alcock Nottingham Rothamsted SdTotalWax_M_mgkg Richard Broughton, Frederic Beaudoin seedMoist_mnirs_pct INRA Anne Laperche Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS LLSleaf_E_pct Yongju Huang, John West University of Hertfordshire Richard Broughton, Frederic Beaudoin SdAvenasterol_Cp_pct Rothamsted John Innes Centre Rachel Wells PedLen_M_cm Rachel Wells John Innes Centre PodWid_M_cm Thomas Alcock, David Salt Nottingham SdCu_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdAlcII_M_mgkg Martin Broadley,Thomas Alcock Nottingham LfCs_M_mgkg 9 perfectly regular SdCd_M_mgkg Nottingham Thomas Alcock, David Salt VertWilt_E_0or1 University of Hertfordshire Yongju Huang, John West LfU_M_mgkg Nottingham Martin Broadley,Thomas Alcock SdPb_M_mgkg Nottingham Thomas Alcock, David Salt weevilsDmg_E_0to9 Anne Laperche INRA Weevils (all species) damage score;charancon INRA Anne Laperche podShat_E_score Pod shattering;Scoring of pods open just before harvest;PODSHAT SdCampesterolDiunsat_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin 8 high to severe damage Yongju Huang, John West University of Hertfordshire LLSleaf_E_0to7 Richard Broughton, Frederic Beaudoin Rothamsted SdStigmasterol_Cp_pct Martin Broadley,Thomas Alcock Nottingham LfBa_M_mgkg University of York Sdy-toc_M_mgkg Swen Langer, Lenka Havlickova Nottingham Martin Broadley,Thomas Alcock LfS_M_mgkg Anne Laperche hailDmg_E_0to9 INRA Hail Damage;HAILDMG Anne Laperche Protein %;Content of proteins in seeds determined by NIRS;Pr INRA protSeedCont_M_pct SdCr_M_mgkg Nottingham Thomas Alcock, David Salt erucicSeedCont_M_pct Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC Anne Laperche INRA Anne Laperche INRA cropAnnot_E_cmt Crop problems;Crop problems during the cycle.;CROP John Innes Centre Rachel Wells PodLen_M_cm Martin Broadley,Thomas Alcock Nottingham LfP_M_mgkg 10-30% 7 Rothamsted SdStigmasterol_M_mgkg Richard Broughton, Frederic Beaudoin Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC Anne Laperche oleicSeedCont_M_pct INRA Nottingham SdZn_M_mgkg Thomas Alcock, David Salt PhomaSK_E_0to7 University of Hertfordshire Yongju Huang, John West INRA Anne Laperche plotHarvest_M_kg Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST University of Hertfordshire Yongju Huang, John West PhomaLS_E_0to3 LfMn_M_mgkg Martin Broadley,Thomas Alcock Nottingham < 10% 9 Nottingham Martin Broadley,Thomas Alcock LfSe_M_mgkg linolenicSeedCont_M_pct INRA Anne Laperche Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC Nottingham Martin Broadley,Thomas Alcock LfMg_M_mgkg LfMo_M_mgkg Martin Broadley,Thomas Alcock Nottingham 2 Nottingham LfAs_M_mgkg Martin Broadley,Thomas Alcock 1 trace Nottingham Thomas Alcock, David Salt SdMg_M_mgkg Nottingham Thomas Alcock, David Salt SNi_M_mgkg high damage 7 severe damage 9 climaticAnnot_E_cmt Anne Laperche INRA Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC INRA Anne Laperche Plot Surface;Surface of the elementary experimental plot;PLOTSURF plotSize_M_m2 3 Nottingham Thomas Alcock, David Salt SdSr_M_mgkg Rothamsted SdPhytosterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdAld_M_mgkg Richard Broughton, Frederic Beaudoin Martin Broadley,Thomas Alcock Nottingham LfCu_M_mgkg Martin Broadley,Thomas Alcock Nottingham LfNi_M_mgkg SdWght_M_mg Rachel Wells John Innes Centre Prot Yield;Protein yield per ha;PrY Anne Laperche INRA protYield_Comp_qha moderately regular 5 PhomaLS_Cou_pct University of Hertfordshire Yongju Huang, John West Richard Broughton, Frederic Beaudoin Rothamsted SdAlk_M_mgkg Rothamsted SdCycloartenol_M_mgkg Richard Broughton, Frederic Beaudoin stearicSeedCont_M_pct INRA Anne Laperche Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC Diseased leaved with a few large lesions of many small leasions, area quite heavily infected 4 no visible affection 0 University of Hertfordshire Yongju Huang, John West VertWilt_Cou_pct BeakLen_M_cm Rachel Wells John Innes Centre 7 regular Thomas Alcock, David Salt Nottingham SdB_M_mgkg plntStdEmerg_M_plt INRA Anne Laperche Plant Emergence;Percentage of seeds that emerge;EMERG PltHght_M_cm Rachel Wells John Innes Centre Thomas Alcock, David Salt Nottingham SdCo_M_mgkg Nottingham Thomas Alcock, David Salt SdLi_M_mgkg INRA phomaDmg_CompG2_0to9 Phoma damage G2 score Anne Laperche 5 75-100% girdling LfFe_M_mgkg Martin Broadley,Thomas Alcock Nottingham ≤ 5% girdling 1 white blight University of Hertfordshire Sclerot_Cou_plnt6plnt Yongju Huang, John West INRA Anne Laperche Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL protMealCont_M_pct acronym Martin Broadley,Thomas Alcock LfCa_M_mgkg Nottingham LfTi_M_mgkg Martin Broadley,Thomas Alcock Nottingham INRA Anne Laperche glsSeedCont_M_μmolg Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS Rachel Wells John Innes Centre FertBraNumb_Ct_Fb Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND INRA Anne Laperche regStand_E_0to9 INRA Anne Laperche seedMoist_mlab_pct Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB SdCa_M_mgkg Thomas Alcock, David Salt Nottingham Growth Stage C1;Bolting date when the first inter-node appears corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1 INRA Anne Laperche stageC1_cnt_dayj Nottingham Martin Broadley, Catherine Thomas MLRL_Cp_cm Swen Langer, Lenka Havlickova Sda-toc_M_mgkg University of York flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4 Anne Laperche INRA stageG4_cnt_dayj Growth Stage G4;30% of the pods have reached their final size Nottingham Martin Broadley,Thomas Alcock LfV_M_mgkg Martin Broadley, Catherine Thomas PRL_M_cm Nottingham SdFe_M_mgkg Thomas Alcock, David Salt Nottingham Anne Laperche elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2 Growth Stage F2;10% of the flowers are open on the main inflorescence stageF2_cnt_dayj INRA Thomas Alcock, David Salt Nottingham SdS_M_mgkg stageG5_cnt_dayj Growth Stage G5;10% of the seeds have reached maturity INRA seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5 Anne Laperche Martin Broadley,Thomas Alcock Nottingham LfRb_M_mgkg Martin Broadley, Catherine Thomas LRL_M_cm Nottingham Yongju Huang, John West University of Hertfordshire LLSpods_E_pct Martin Broadley,Thomas Alcock Nottingham LfCd_M_mgkg no symptoms observable 0 Rothamsted SdCampesterol diunsat_Cp_pct Richard Broughton, Frederic Beaudoin LRN_Ct_root Martin Broadley, Catherine Thomas Nottingham high > 90% 1 Evidence of stunting 6 Thomas Alcock, David Salt Nottingham SdP_M_mgkg Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO stageFlo_cnt_dayj INRA Anne Laperche LfAl_M_mgkg Nottingham Martin Broadley,Thomas Alcock PodNumbTermRac_Ct_PodTermRac Rachel Wells John Innes Centre low to intermediate damage 4 Martin Broadley,Thomas Alcock LfPb_M_mgkg Nottingham INRA Anne Laperche plntStnd_M_plntm2 Plant Density;Number of viable plants counted per surface unit;PLANTDENS John Innes Centre FtLen_M_cm Rachel Wells LfB_M_mgkg Martin Broadley,Thomas Alcock Nottingham Yongju Huang, John West PhomaLS_Cou_plnt10plnt University of Hertfordshire flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1 Growth Stage G1;10% of the pods have reached their final size INRA Anne Laperche stageG1_cnt_dayj Plant Lodging;Scoring of the plant lodging of the plot;LODGING Anne Laperche INRA lodging_E_0to9 100% girdling ( stem weak/firm) 6 Richard Broughton, Frederic Beaudoin SdFA_M_mgkg Rothamsted PhomaSKbase_E_0to7 University of Hertfordshire Yongju Huang, John West 2 5-10 spots per plant 4 SdAs_M_mgkg Thomas Alcock, David Salt Nottingham SD_Est_mm Nottingham Martin Broadley, Catherine Thomas 60-90% 3 Rothamsted SdCampesterol_M_mgkg Richard Broughton, Frederic Beaudoin intermediate to high damage 6 Nottingham LfSr_M_mgkg Martin Broadley,Thomas Alcock Half green/half diseased, leaf curling and distortion 5 PhomaSKupper_E_0to7 Yongju Huang, John West University of Hertfordshire INRA Anne Laperche Plant Size;Height of plants in an experimental plot;PLANTSIZE plntHeight_M_cm LfK_M_mgkg Martin Broadley,Thomas Alcock Nottingham 100% girdling (stem dead/lodged)) 7 Richard Broughton, Frederic Beaudoin SdBranched_M_mgkg Rothamsted >10 spots per plant 3 <5 spots per plant 1 SdB-Sitosterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdNum_Ct_sd John Innes Centre Rachel Wells University of Hertfordshire Yongju Huang, John West LLSstem_E_pct 3 Plants with scattered lesions Richard Broughton, Frederic Beaudoin Rothamsted SdAlcI_M_mgkg Culture leaning, without impact 2 University of Hertfordshire LeafMiner_Cou_pct Yongju Huang, John West Trace of disease 1 irregular 3 2 Diseased leaves with non or a few small lesions Brassica ontology Brassica napus ontology SdKeto_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin very low damage 2 1 high irregular 7 Plants heavily stunted or dead. Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY Anne Laperche stagePodMaturity_cnt_dayj INRA intermediate damage 5 3 low damage attribute 0 No leaf spotting 51-75% girdling 4 8 26-50% girdling 3 0 NA 4 NA 0 6 0 NA entity Seed moisture content humidifier - Measurement Humidimeter (sample of seeds) Leaf Nickel content Nickel content LfNi Leaf The concentration of Nickel in leaves length foot Length of lower stem from ground level to last formed fertile branch Foot length Primary root lengh Primary root length PRL Length of the primary root Oil yield - Computation Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O) Caesium content Leaf Caesium content The concentration of Caesium in leaves LfCs Leaf Titanium content LfTi The concentration of Titanium in leaves Leaf Leaf Titanium content Stand Plant density PltSd Plant Number of viable plants counted per surface unit Crop stand Plant stand 10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%. LLS incidence (diseased) - Estimation Development stage pod development stage;time to BBCH growth stages 72-79 Additional stages for pod development according to the BBCH scale (72-79) Plant Pod development time (BBCH 72-79) 3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg Seed mineral analysis - Measurement LfPb The concentration of Lead in leaves Lead content Leaf Lead content Leaf 10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated. LLS severity - Estimation plants/10 plants Plant Oil yield Oil yield OY Oil yield LfAs Leaf Arsenic content The concentration of Arsenic in leaves Leaf Arsenic content The concentration of Boron in leaves Boron content Leaf Boron content Leaf LfB Leaf mineral analysis - Measurement 3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry Beginning of pod maturity (BBCH 81) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Seed Stearic acid content The concentration of stearic acid in seeds. SdStearic Seed stearic acid content LLS severity - Estimation 10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated. Leaf The concentration of Potassium in leaves Leaf Potassium content LfK Potassium content day-of-year Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS. LLS severity on pods (percentage of pod area covered by LLS) Light leaf spot severity Pod The percentage of Stigmasterol in the total of Phytosterols in seeds Stigmasterol proportion of Phytosterols SdStigmasterol_Cp Seed Sigmasterol content Stigmasterol Seed Pedicel length of pods from terminal raceme pedicel length Pedicel length mg/kg weight Seed weight seed Seed weight of ten typical pods Height is measured from ground to the top of the plants using a dipstick graduated in cm Plant height - Measurement A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester plotharvest - Measurement Seed moisture content. Seed moisture content Seed Moisture content SdMoist Protein content The concentration of proteins in the meal. MlProt Meal Meal protein content Biomass yield Biomass Weight (kg) of raw materials harvested by plot Yield CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of pod development (BBCH 71) date – Estimation GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement * Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation. * Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step. * 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples. * Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour. * Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials. * A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions. * The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes. * 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used). * The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane. * Samples are vortexed, centrifuged and the upper hexane phase is removed. * A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first. * Samples are dried in a rotary evaporator or under a stream of nitrogen. * Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID). *GC program: * Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes. * Inlet temp: 325°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C Pod PodShat Shattering Pod shattering Proportion of open pods (assessment) CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod development stages (BBCH 72-79) date – Estimation HailDmg - Estimation Score the damage caused by hail. Seed Campesterol content The concentration of Campesterol in seeds Seed Campesterol SdCampesterol Campesterol content Pod Number - Counting Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Stem length - Measurement Seed moisture content. Seed moisture content Seed Moisture content SdMoist Plant lodging incidence - Estimation Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging Plant Absence or presence of powdery mildew Presence (1) or absence (0) of powdery mildew Powdery mildew presence MLRL The mean length of a lateral root of that root system Mean lateral root length Lateral root average length Powdery mildew presence - Estimation Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1 Evaluation of the Phoma lingam infection with the G2 score. The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation. Phoma damage – G2 score – Computation Crop problems annotations - Estimation Visual observations Avenasterol SdAvenasterol_Cp Seed Avenasterol proportion of Phytosterols Seed Avenasterol content The percentage of Avenasterol in the total of Phytosterols in seeds Stem Phoma stem cancer severity Phoma stem canker severity Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa method_of SdErucic Seed The concentration of erucic acid in seeds. Seed erucic acid content Erucid acid content Percentage of seeds that emerge. PltSdEm Plant stand at emergence Plant emergence Seed Phoma leaf spots The number of plants that showed presence of phoma leaf spots from a subsample of a plot Plant Number of plants with phoma leaf spots The concentration of all compounds with branched alcyl chains from pod and stem material branched Alcyl-chain content Pod and stem SdBranched Pod and stem branched Alcyl-chain content GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement * 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.). * Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers. * Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C. * Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. * After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS. * GC program: * Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes. * Inlet temp: 280°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C Seed Nitrogen Harvest Index Seed N content to total plant N content ratio NHI N content proportion of plant N content Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root) Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr) Seed protein Yield - Computation Plant Verticilium wilt presence The percentage of plants that showed presence of leaf miner from a subsample of a plot Verticillium wilt incidence SD Seed diameter The diameter of a seed Seed diameter % Plant BBCH growth stage 69;end flowering stage Development stage End of flowering time (BBCH 69) End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale) alpha-tocopherol content Alpha- tocopherol content in seeds Sda-toc seed Seed alpha- tocopherol content Pod and stem secondary Alkohol functional group content Pod and stem secondary Alkohol functional group content The concentration of components with secondary alcohol functional groups from pod and stem material SdAlcII Protein content The concentration of proteins in seeds. Seed protein content Seed SdProt cm SdCycloartenol_Cp Cycloartenol proportion of Phytosterols Seed Seed Cycloartenol content The percentage of Cycloartenol in the total of Phytosterols in seeds Cycloartenol Glucosinolate content Seed Seed glucosinolate content SdGLS The concentration of glucosinolates in seeds. q/ha StOleic The concentration of oleic acid in seeds. Oleic acid content Seed Seed oleic acid content BBCH growth stage 71;CETIUM growth stage G1 Plant 10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale) Beginning of pod development time (BBCH 71) Development stage The concentration of Cycloartenol in seeds Seed SdCycloartenol Seed Cycloartenol content Cycloartenol Cycloartenol content SdSe Selenium content The concentration of Selenium in seeds Seed Selenium content Seed LfMo Molybdenum content Leaf Molybdenum content The concentration of Molybdenum in leaves Leaf Maximum plant height from ground to tip of terminal raceme plant Plant height height PH Lateral root LRD Lateral root density density The mean density of lateral roots along the primary root Seed Brassicasterol content Brassicasterol content The concentration of Brassicasterol in seeds Seed SdBrassicasterol Brassicasterol The concentration of Rubidium in seeds Rubidium content SdRb Seed Rubidium content Seed Image analysis - Counting RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN). The concentration of Sulphur in seeds SdS Seed Seed Sulphur content Sulphur content Calcium content Seed Calcium content The concentration of Calcium in seeds Seed SdCa The concentration of Lead in seeds SdPb Lead content Seed Lead content Seed Seed delta- tocopherol content Delta- tocopherol content in seeds seed Sdd-toc delta-tocopherol content Pod width - Measurement Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pedicel length - Measurement Plant Beginning of pod maturity time (BBCH 81) BBCH growth stage 81;CETIUM growth stage G5 10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale) Development stage variable_of LfCd The concentration of Cadmium in leaves Cadmium content Leaf Leaf Cadmium content Seed Seed Cobalt content The concentration of Cobalt in seeds Cobalt content SdCo Linoleic acid content Seed Seed linoleic acid content The concentration of linoleic acid in seeds. StLinoleic plant/m2 Phoma leaf spot severity Severity assessment of phoma leaf spot Leaf phoma resistance Leaf NIRS assay. Pct of Seed Dry Weight Protein meal content NIRS - Measurement NIRS assay. Pct total fatty acids Linoleic seed content NIRS - Measurement Weevil damage - Estimation Observe damage caused by Weevils (all species), and rate the damage. 0-5 percentage bracket scale boolean Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter. Seed sieving - Estimation Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. No of plants with Sclerotinia (out of six) Sclerotinia incidence Plant Pod length of pods from terminal raceme Pod length length pod NIRS assay Seed moisture NIRS - Measurement Leaf Iron content Iron content LfFe The concentration of Iron in leaves Leaf CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Addictional flowering stages (BBCH 62-65) date – Estimation μmol/g 0-9 Lodging Incidence scale Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod length - Measurement mm Stand Plant Regularity of plant stand Scoring of the regularity of the plant stand SdNi Seed The concentration of Nickel in seeds Nickel content Seed Nickel content Seed Strontium content SdSr The concentration of Strontium in seeds Seed Strontium content Seed Copper content Seed Copper content The concentration of Copper in seeds SdCu First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale) Development stage First flowers time (BBCH 60) BBCH growth stage 60;CETIUM growth stage F1 Plant B-Sitosterol content SdB-Sitosterol Seed B-Sitosterol content B-Sitosterol Seed The concentration of B-Sitosterol in seeds primary Alkohol functional group content SdAlcI Pod and stemprimary Alkohol functional group content The concentration of components with primary alcohol functional groups from pod and stem material Pod and stem Plant BBCH growth stage 73;CETIUM growth stage G4 Development stage 30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale) Early pod development time (BBCH 73) Seed Oil content Seed oil content The concentration of oil in seeds. SdOil Additionnal pod maturity stages (BBCH 82-99) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. LfBa The concentration of Balium in leaves Leaf Leaf Balium content Balium content WeevilsDmg Weevils damage Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil). Weevils damage Plant Exposure to climatic stress Plant ClimPb Climatic incidents annotation Climatic incidents during the crop cycle. Nitrate Harvest index - Computation Samples from HI assay are analysed for N_%DW in the different fractions NIRS assay. Pct total fatty acids Erucid seed content NIRS - Measurement The concentration of Cadmium in seeds Seed SdCd Cadmium content Seed Cadmium content Stem LLS severity on stem (percentage of stem area covered by LLS) Light leaf spot severity on stems, judged by the percentage of area covered by LLS. Light leaf spot severity The concentration of Manganese in seeds Seed Manganese content Seed SdMn Manganese content LfCa Calcium content Leaf Calcium content The concentration of Calcium in leaves Leaf 0-9 Damage/Incidence scale Assessment on measure validity. Plot Valid Measure validity Plot measure validity Leaf Rubidium content The concentration of Rubidium in leaves Leaf Rubidium content LfRb The concentration of components with alkane functional groups from pod and stem material SdAlk Pod and stem Pod and stem Alkane-functional group content Alkane-functional group content Strontium content Leaf Strontium content Leaf LfSr The concentration of Strontium in leaves Plant stand - Counting Count the number of plants on linear sections defined in each sowing line at the exception of the border lines 0-7 percentage scale The concentration of Boron in seeds Boron content Seed SdB Seed Boron content Pod and stem SdKeto Pod and stem Ketone functional group content The concentration of components with ketone functional groups from pod and stem material Ketone functional group content Leaf Incidence with leaf miner The percentage of plants that showed presence of leaf miner from a subsample of a plot Leaf miner incidence Exposure to biotic attack; damage du to biotic attack BioticPb Plant Biotic attack during the crop cycle. Biotic attack annotation Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image. Stem witdh - Measurement beak length Pod beak length Beak length A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents HPLC (High performance liquid chromatography) analysis - Measurement Plant height - Measurement Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Leaf Sodium content LfNa Sodium content Leaf The concentration of Sodium in leaves Visual notations (scale 0 - 9) regularity stand - Estimation The concentration of Manganese in leaves Leaf Manganese content Leaf LfMn Manganese content Absence or presence of Verticilium wilt on the plant Verticilium wilt presence Verticilium wilt presence Plant Verticilium wilt presence - Counting Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded. width Pod width of pods from terminal raceme Pod width pod SdTotalWax The concentration of wax in the pod and seed material wax content Pod and stem Pod and stem wax content Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) NIRS assay. Pct total fatty acids Oleic seed content NIRS - Measurement 10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7. LLS resistance - Estimation Stearic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Seed palmitic acid content SdPalmitic The concentration of palmitic acid in seeds. Seed Palmitic acid content 10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner. Leaf miner incidence (diseased) - Counting root LfZn Zinc content Leaf The concentration of Zinc in leaves Leaf Zinc content Lodg The plant lodging incidence. Plant lodging incidence Lodging incidence Plant Pod and stem The concentration of components with fatty acids as functional groups from pod and stem material Fatty acids functional group content SdFA Pod and stem Fatty acids functional group content SdAvenasterol Avenasterol content Seed Seed Avenasterol content The concentration of Avenasterol in seeds Avenasterol Image analysis - Computation The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001) The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot Leaf Phoma incidence Incidence of Phoma leaf spot Leaf LfV Leaf Vanadium content Vanadium content The concentration of Vanadium in leaves Plant Additional stages for pod maturity according to the BBCH scale (82-99) Development stage pod maturity stage;time to BBCH growth stage 82-99 Pod maturity time (BBCH 82-99) Phoma damage Plant Withering disease PhomaDmg Withering disease caused by the agent Leptosphaeria maculans (Phoma). Phoma damage LfCu Leaf Copper content Leaf Copper content The concentration of Copper in leaves gamma-tocopherol content Seed gamma- tocopherol content seed Sdy-toc Gamma- tocopherol conten in seeds Stigmasterol content SdStigmasterol The concentration of Stigmasterol in seeds Seed Sigmasterol content Stigmasterol Seed Palmitic seed content NIRS - Measurement NIRS assay. Pct total fatty acids SdMo Seed Molybdenum content Molybdenum content The concentration of Molybdenum in seeds Seed number Seed number seed Number of seeds in ten typical pods Number of lateral roots number Lateral root number LRN Lateral root Uranium content Leaf Uranium content LfU The concentration of Uranium in leaves Leaf flowering stage;time to BBCH growth stage 62-65;Anthesis Development stage Plant Flowering time (BBCH 62-65) Additional flowering stages according to the BBCH scale (62-65) SdLi Seed Seed Lithium content The concentration of Lithium in seeds Lithium content The concentration of Sulphur in leaves Leaf Sulphur content Leaf LfS Sulphur content SdPhytosterol Seed Seed total Phytosterol content The total concentration of phytosterols in seeds Phytosterol content Comment Phoma stem canker base stem score Phoma stem cancer severity Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Stem base plants/6 plants seed The concentration of Sodium in seeds Sodium content SdNa Seed Sodium content Seed Climatic incidents annotions - Estimation Visual observations Brassicasterol proportion of Phytosterols Seed Brassicasterol Seed Brassicasterol content SdBrassicasterol The percentage of Brassicasterol in the total of Phytosterols in seeds Lateral root length Lateral root length Length of the lateral root LRL diunsaturated Campesterol content Seed diunsaturated Campesterol content SdCampesterolDiunsat Campesterol diunsat The concentration of diunsaturated Campesterol in seeds Seed Light leaf spot resistance Leaf Light Leaf spot severity on leafs LLS severity on leaf NIRS assay Quantity per Seed Dry Weight GLS seed content NIRS - Measurement Plot size Surface of the elementary experimental plot Size Plot PlotSize The concentration of Phosphorus in leaves Leaf LfP Phosphorus content Leaf Phosphorus content Image analysis - Computation The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001) Seed SdFe Iron content The concentration of Iron in seeds Seed Iron content The concentration of components with aldehyde functional groups from pod and stem material Pod and stem SdAld Aldehyde functional group content Pod and stem Aldehyde functional group content Linolenic acid content Seed linolenic acid content Seed The concentration of linolenic acid in seeds. StLinolenic Scoring of presence or absence of verticilium wilt symptoms across whole plant. Verticilium wilt presence - Estimation Protein seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight Plot measure validity – Estimation Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid” Seed moisture content – Computation Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively Phoma stem canker upper stem lesion score Upper stem Phoma stem cancer severity Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Leaf Leaf Silver content The concentration of Silver in leaves LfAg Silver content 0-9 Regularity scale The concentration of Selenium in leaves Leaf Selenium content LfSe Leaf Selenium content CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First flowers (BBCH 60) date – Estimation The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols Relative seed Phytosterol content - Computation Fertile Branches - Counting Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. End of flowering (BBCH 69) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. kg Image analysis - Computation Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001) CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of flowering (BBCH 61) date - Estimation Seed Seed Potassium content The concentration of Potassium in seeds SdK Potassium content Seed SdMg The concentration of Magnesium in seeds Seed Magnesium content Magnesium content Beginning of flowering time (BBCH 61) 10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale) BBCH growth stage 61;CETIUM growth stage F2 Development stage Plant Visual notations (scale 0 - 9) Pod shattering - Estimation Biotic stress annotations - Estimation Visual observations Campesterol diunsat The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds Campesterol diunsat proportion of Phytosterols SdCampesterolDiunsat_Cp Seed diunsaturated Campesterol content Seed Total root length TRL Length of all roots Total root length Phoma incidence (diseased) - Counting 10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot. mg SdZn Seed Zinc content Seed The concentration of Zinc in seeds Zinc content Seed SdCr Seed Chromium content Chromium content The concentration of Chromium in seeds Seed impurity content Impurities content Seed SdImpur Percentage of impurities in a sample of seeds determined by weighing. Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phosphorus content Seed Phosphorus content dSP Seed The concentration of Phosphorus in seeds 10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded Phoma leaf spot incidence (diseased) - Estimation Protein yield Protein yield per ha Seed protein yield PrY Seed Chromium content The concentration of Chromium in leaves Leaf Chromium content Leaf LfCr pod/20cm of terminal raceme number Number of fertile branches Number of fertile branches fertile branch Plant stand at emergence – Computation EMERG = (Number of plants obtained / Number of seeds sown) x 100 Seed Number - Counting Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First inter-node (BBCH 31) date – Estimation First inter-node time (BBCH 31) Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale) Development stage Plant BBCH growth stage 31;CETIUM growth stage C1 Seed impurity content – Computation Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively Stem width stem width Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant. Pod beak length - Measurement Leaf Aluminium content Leaf The concentration of Aluminium in leaves LfAl Aluminium content Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. Sclerotinia incidence Plant No of plants with Sclerotinia (per plot) Leaf phoma resistance - Estimation 10 spots per plant]]> LfMg Leaf Magnesium content Magnesium content Leaf The concentration of Magnesium in leaves Linolenic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Seed Campesterol content The percentage of Campesterol in the total of Phytosterols in seeds Seed Campesterol SdCampesterol_Cp Campesterol proportion of Phytosterols fertile branch Sclerotinia incidence (diseased) - Counting The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded. root/cm Pod number number Number of pods on terminal raceme pod Plant damage caused by hail. Plant HailDmg Hail damage Hail damage 0-3 disease stage scale Crop problems during the crop cycle. Crop problems annotation CropPb Plant Exposure to crop problems;damage from crop problem CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Early pod development (BBCH 73) date – Estimation Arsenic content Seed The concentration of Arsenic in seeds Seed Arsenic content SdAs Seed weight - Measurement Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. The percentage of plants that showed presence of light leaf spot incidence on leaves LLS incidence on leaf Leaf Light leaf spot incidence Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate). Sclerotinia incidence (diseased) - Counting SdB-Sitosterol_Cp B-Sitosterol Seed The percentage of B-Sitosterol in the total of Phytosterols in seeds B-Sitosterol proportion of Phytosterols Seed B-Sitosterol content scale_of Plot Size – Computation Surface is determined by the experimental design 0-7 disease stage scale plants/plot 2020-10-19T00:00:02 Oil seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight