oilSeedCont_M_pct Oil %;Content of oil in seeds determined by NIRS;O INRA Anne Laperche Nottingham LfMn_M_mgkg Martin Broadley,Thomas Alcock Rothamsted SdPhytosterol_M_mgkg Richard Broughton, Frederic Beaudoin LfAs_M_mgkg Nottingham Martin Broadley,Thomas Alcock Anne Laperche flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4 stageG4_cnt_dayj Growth Stage G4;30% of the pods have reached their final size INRA Nottingham Martin Broadley,Thomas Alcock LfK_M_mgkg INRA Anne Laperche seedMoist_mlab_pct Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS Anne Laperche INRA seedMoist_mnirs_pct 5 Half green/half diseased, leaf curling and distortion Plant Lodging;Scoring of the plant lodging of the plot;LODGING lodging_E_0to9 INRA Anne Laperche PhomaSK_E_0to7 University of Hertfordshire Yongju Huang, John West SdMo_M_mgkg Thomas Alcock, David Salt Nottingham podShat_E_score Pod shattering;Scoring of pods open just before harvest;PODSHAT INRA Anne Laperche SdCd_M_mgkg Nottingham Thomas Alcock, David Salt 9 < 10% University of Hertfordshire PhomaLS_E_0to3 Yongju Huang, John West Martin Broadley,Thomas Alcock LfP_M_mgkg Nottingham LLSstem_E_pct University of Hertfordshire Yongju Huang, John West INRA Anne Laperche stageFlo_cnt_dayj Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO 2 very low damage John Innes Centre Rachel Wells PodLen_M_cm NA 0 University of Hertfordshire white blight Yongju Huang, John West Sclerot_Cou_plntPlot LfBa_M_mgkg Nottingham Martin Broadley,Thomas Alcock Martin Broadley,Thomas Alcock Nottingham LfS_M_mgkg Plant Size;Height of plants in an experimental plot;PLANTSIZE plntHeight_M_cm INRA Anne Laperche SdBrassicasterol_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin Rothamsted SdBrassicasterol_Cp_pct Richard Broughton, Frederic Beaudoin 5 moderately regular LRD_Cp_rootcm Martin Broadley, Catherine Thomas Nottingham Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY INRA stagePodMaturity_cnt_dayj Anne Laperche John Innes Centre BeakLen_M_cm Rachel Wells 3 Plants with scattered lesions Anne Laperche Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC palmiticSeedCont_M_pct INRA Nottingham Thomas Alcock, David Salt SdNa_M_mgkg University of Hertfordshire PhomaLS_Cou_pct Yongju Huang, John West 1 Trace of disease 2-25% girdling 2 white blight Yongju Huang, John West University of Hertfordshire Sclerot_Cou_plnt6plnt LfB_M_mgkg Nottingham Martin Broadley,Thomas Alcock <5 spots per plant 1 LfMg_M_mgkg Martin Broadley,Thomas Alcock Nottingham 3 26-50% girdling LfSr_M_mgkg Nottingham Martin Broadley,Thomas Alcock seedMoistHarv_mhumi_pct Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV Anne Laperche INRA Growth Stage End Flowering;End of flowering on the main inflorescence corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO INRA Anne Laperche stageEndFlo_cnt_dayj Nottingham TRL_Cp_cm Martin Broadley, Catherine Thomas Richard Broughton, Frederic Beaudoin Rothamsted SdStigmasterol_Cp_pct Anne Laperche INRA erucicSeedCont_M_pct Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC Richard Broughton, Frederic Beaudoin SdB-Sitosterol_Cp_pct Rothamsted Nottingham SdSe_M_mgkg Thomas Alcock, David Salt plotHarvest_M_kg Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST INRA Anne Laperche Nottingham Martin Broadley,Thomas Alcock LfNa_M_mgkg climaticAnnot_E_cmt INRA Anne Laperche Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC Weevils (all species) damage score;charancon weevilsDmg_E_0to9 INRA Anne Laperche Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV stagePodDev_cnt_dayj INRA Anne Laperche INRA Anne Laperche stageG1_cnt_dayj flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1 Growth Stage G1;10% of the pods have reached their final size LeafMiner_Cou_pct Yongju Huang, John West University of Hertfordshire Brassica napus ontology Brassica ontology Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI NHI_Comp_pct INRA Anne Laperche 2 Culture leaning, without impact Thomas Alcock, David Salt Nottingham SdB_M_mgkg Rothamsted SdCycloartenol_M_mgkg Richard Broughton, Frederic Beaudoin linolenicSeedCont_M_pct INRA Anne Laperche Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC LfCs_M_mgkg Nottingham Martin Broadley,Thomas Alcock regStand_E_0to9 INRA Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND Anne Laperche INRA Plant Emergence;Percentage of seeds that emerge;EMERG Anne Laperche plntStdEmerg_M_plt University of Hertfordshire Yongju Huang, John West PowMildew_E_0or1 Thomas Alcock, David Salt SdMg_M_mgkg Nottingham Anne Laperche Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL protMealCont_M_pct INRA Rothamsted Richard Broughton, Frederic Beaudoin SdKeto_M_mgkg Rothamsted SdTotalWax_M_mgkg Richard Broughton, Frederic Beaudoin 4 51-75% girdling SdCa_M_mgkg Nottingham Thomas Alcock, David Salt Anne Laperche protSeedCont_M_pct Protein %;Content of proteins in seeds determined by NIRS;Pr INRA Rothamsted SdAld_M_mgkg Richard Broughton, Frederic Beaudoin LfCu_M_mgkg Nottingham Martin Broadley,Thomas Alcock Rothamsted SdAvenasterol_Cp_pct Richard Broughton, Frederic Beaudoin Yongju Huang, John West LLSleaf_E_0to7 University of Hertfordshire 1 No lodging Growth Stage G5;10% of the seeds have reached maturity seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5 stageG5_cnt_dayj Anne Laperche INRA Rothamsted SdB-Sitosterol_M_mgkg Richard Broughton, Frederic Beaudoin Thomas Alcock, David Salt SdCr_M_mgkg Nottingham SdSr_M_mgkg Thomas Alcock, David Salt Nottingham Martin Broadley, Catherine Thomas PRL_M_cm Nottingham Prot Yield;Protein yield per ha;PrY INRA Anne Laperche protYield_Comp_qha Thomas Alcock, David Salt Nottingham SdFe_M_mgkg Martin Broadley,Thomas Alcock LfU_M_mgkg Nottingham INRA Anne Laperche hailDmg_E_0to9 Hail Damage;HAILDMG elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2 Anne Laperche INRA Growth Stage F2;10% of the flowers are open on the main inflorescence stageF2_cnt_dayj Rothamsted SdAlcII_M_mgkg Richard Broughton, Frederic Beaudoin high to severe damage 8 Anne Laperche Oil Yield;Oil yield per ha;OY oilYield_Comp_qha INRA Martin Broadley, Catherine Thomas LRL_M_cm Nottingham INRA corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1 Growth Stage C1;Bolting date when the first inter-node appears Anne Laperche stageC1_cnt_dayj Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC INRA Anne Laperche linoleicSeedCont_M_pct 0 No leaf spotting Anne Laperche Phoma damage G2 score phomaDmg_CompG2_0to9 INRA Rothamsted Richard Broughton, Frederic Beaudoin SdAvenasterol_M_mgkg University of Hertfordshire Yongju Huang, John West PhomaLS_Cou_plnt10plnt Average loding 5 Nottingham LfMo_M_mgkg Martin Broadley,Thomas Alcock Nottingham Martin Broadley,Thomas Alcock LfTi_M_mgkg Thomas Alcock, David Salt Nottingham SdAs_M_mgkg University of Hertfordshire Yongju Huang, John West PhomaSKupper_E_0to7 Sdy-toc_M_mgkg Swen Langer, Lenka Havlickova University of York Yongju Huang, John West LLSpods_E_pct University of Hertfordshire severe damage 9 Nottingham SdCu_M_mgkg Thomas Alcock, David Salt irregular 3 glsSeedCont_M_μmolg INRA Anne Laperche Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS Thomas Alcock, David Salt Nottingham SdP_M_mgkg attribute LfRb_M_mgkg Nottingham Martin Broadley,Thomas Alcock Rachel Wells FertBraNumb_Ct_Fb John Innes Centre Richard Broughton, Frederic Beaudoin SdAlk_M_mgkg Rothamsted Yongju Huang, John West VertWilt_Cou_pct University of Hertfordshire low to intermediate damage 4 INRA Plot Surface;Surface of the elementary experimental plot;PLOTSURF Anne Laperche plotSize_M_m2 Plants heavily stunted or dead. 7 Nottingham Martin Broadley,Thomas Alcock LfZn_M_mgkg Nottingham Martin Broadley,Thomas Alcock LfAg_M_mgkg John Innes Centre PedLen_M_cm Rachel Wells 7 10-30% StemWid_M_cm John Innes Centre Rachel Wells Rachel Wells PodNumbTermRac_Ct_PodTermRac John Innes Centre John Innes Centre Rachel Wells FtLen_M_cm Swen Langer, Lenka Havlickova Sdd-toc_M_mgkg University of York 0 no visible affection 3 low damage 1 trace Richard Broughton, Frederic Beaudoin SdBranched_M_mgkg Rothamsted SdZn_M_mgkg Thomas Alcock, David Salt Nottingham Nottingham SdLi_M_mgkg Thomas Alcock, David Salt John Innes Centre PodWid_M_cm Rachel Wells 9 perfectly regular SdS_M_mgkg Thomas Alcock, David Salt Nottingham Richard Broughton, Frederic Beaudoin SdCampesterol diunsat_Cp_pct Rothamsted Rachel Wells SdWght_M_mg John Innes Centre Martin Broadley, Catherine Thomas Nottingham MLRL_Cp_cm INRA Anne Laperche bioticAnnot_an_text Biotic Stress;Biotic attack during the crop cycle;BIOTIC Nottingham Martin Broadley,Thomas Alcock LfFe_M_mgkg Rothamsted SdCampesterol_M_mgkg Richard Broughton, Frederic Beaudoin Evidence of stunting 6 LfV_M_mgkg Nottingham Martin Broadley,Thomas Alcock INRA Anne Laperche plntStnd_M_plntm2 Plant Density;Number of viable plants counted per surface unit;PLANTDENS SdK_M_mgkg Thomas Alcock, David Salt Nottingham Richard Broughton, Frederic Beaudoin Rothamsted SdCampesterolDiunsat_M_mgkg LfCa_M_mgkg Nottingham Martin Broadley,Thomas Alcock >10 spots per plant 3 John Innes Centre Rachel Wells PltHght_M_cm Thomas Alcock, David Salt Nottingham SdRb_M_mgkg University of Hertfordshire PhomaSKbase_E_0to7 Yongju Huang, John West 1 ≤ 5% girdling INRA Anne Laperche validateannot_E_cmt Annotations on measure validity;VALIDATION INRA Anne Laperche oleicSeedCont_M_pct Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC Martin Broadley,Thomas Alcock LfCd_M_mgkg Nottingham Nottingham Martin Broadley,Thomas Alcock LfCr_M_mgkg John Innes Centre SdNum_Ct_sd Rachel Wells 0 NA Rothamsted Richard Broughton, Frederic Beaudoin SdFA_M_mgkg 6 intermediate to high damage LfSe_M_mgkg Nottingham Martin Broadley,Thomas Alcock VertWilt_E_0or1 University of Hertfordshire Yongju Huang, John West Rothamsted SdAlcI_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Richard Broughton, Frederic Beaudoin SdCampesterol_Cp_pct LfNi_M_mgkg Martin Broadley,Thomas Alcock Nottingham Swen Langer, Lenka Havlickova Sda-toc_M_mgkg University of York acronym Martin Broadley,Thomas Alcock LfAl_M_mgkg Nottingham Crop problems;Crop problems during the cycle.;CROP Anne Laperche cropAnnot_E_cmt INRA LfPb_M_mgkg Nottingham Martin Broadley,Thomas Alcock INRA Anne Laperche Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC stearicSeedCont_M_pct 8 0 no symptoms observable 1 high irregular University of Hertfordshire LLSleaf_E_pct Yongju Huang, John West seedImpur_M_pct Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR INRA Anne Laperche high damage 7 Martin Broadley, Catherine Thomas SD_Est_mm Nottingham 4 Nottingham SNi_M_mgkg Thomas Alcock, David Salt entity Thomas Alcock, David Salt SdMn_M_mgkg Nottingham 5 intermediate damage Rothamsted Richard Broughton, Frederic Beaudoin SdStigmasterol_M_mgkg SdCo_M_mgkg Thomas Alcock, David Salt Nottingham Nottingham SdPb_M_mgkg Thomas Alcock, David Salt INRA stageF1_cnt_dayj Anne Laperche corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1 Growth Stage F1;First flowers are open LRN_Ct_root Martin Broadley, Catherine Thomas Nottingham Diseased leaves with non or a few small lesions 2 Rothamsted Richard Broughton, Frederic Beaudoin SdCycloartenol_Cp_pct 6 2 5-10 spots per plant 60-90% 3 4 2 5 30-60% 0 No disease observable Diseased leaved with a few large lesions of many small leasions, area quite heavily infected 4 5 75-100% girdling 1 high > 90% NA 0 100% girdling (stem dead/lodged)) 7 100% girdling ( stem weak/firm) 6 7 regular 3 Campesterol diunsat SdCampesterolDiunsat Seed diunsaturated Campesterol content diunsaturated Campesterol content Seed The concentration of diunsaturated Campesterol in seeds Seed delta- tocopherol content delta-tocopherol content Sdd-toc Delta- tocopherol content in seeds seed % Plant height plant height Maximum plant height from ground to tip of terminal raceme PH * 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.). * Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers. * Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C. * Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. * After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS. * GC program: * Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes. * Inlet temp: 280°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement q/ha The concentration of Arsenic in seeds Seed Arsenic content SdAs Seed Arsenic content SdBranched branched Alcyl-chain content Pod and stem branched Alcyl-chain content Pod and stem The concentration of all compounds with branched alcyl chains from pod and stem material SdPb Lead content Seed Lead content Seed The concentration of Lead in seeds PhomaDmg Withering disease Phoma damage Phoma damage Withering disease caused by the agent Leptosphaeria maculans (Phoma). Plant 0-9 Damage/Incidence scale root/cm Seed weight - Measurement Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. The concentration of linoleic acid in seeds. Linoleic acid content StLinoleic Seed linoleic acid content Seed Pod and stem Fatty acids functional group content Fatty acids functional group content The concentration of components with fatty acids as functional groups from pod and stem material Pod and stem SdFA SdMg Seed Magnesium content Seed The concentration of Magnesium in seeds Magnesium content Uranium content The concentration of Uranium in leaves LfU Leaf Uranium content Leaf Crop problems during the crop cycle. CropPb Exposure to crop problems;damage from crop problem Plant Crop problems annotation NIRS assay. Pct of Seed Dry Weight Protein meal content NIRS - Measurement Leaf mineral analysis - Measurement 3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry length TRL Total root length Total root Length of all roots Plant Beginning of flowering time (BBCH 61) BBCH growth stage 61;CETIUM growth stage F2 10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale) Development stage boolean Plant stand - Counting Count the number of plants on linear sections defined in each sowing line at the exception of the border lines Pedicel length - Measurement Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Plant height - Measurement plants/10 plants Meal protein content Meal Protein content The concentration of proteins in the meal. MlProt NHI Nitrogen Harvest Index Seed N content to total plant N content ratio Seed Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root) N content proportion of plant N content Seed impurity content Seed Percentage of impurities in a sample of seeds determined by weighing. Impurities content SdImpur ClimPb Climatic incidents during the crop cycle. Plant Climatic incidents annotation Exposure to climatic stress MLRL average length Mean lateral root length The mean length of a lateral root of that root system Lateral root mg/kg seed Lateral root number number Number of lateral roots LRN Lateral root 0-7 percentage scale RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN). Image analysis - Counting 0-7 disease stage scale Campesterol proportion of Phytosterols The percentage of Campesterol in the total of Phytosterols in seeds Seed Campesterol content SdCampesterol_Cp Seed Campesterol Relative seed Phytosterol content - Computation The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols Seed mineral analysis - Measurement 3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg Leaf LLS incidence on leaf The percentage of plants that showed presence of light leaf spot incidence on leaves Light leaf spot incidence Plant Verticilium wilt presence Verticillium wilt incidence The percentage of plants that showed presence of leaf miner from a subsample of a plot Pod Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS. LLS severity on pods (percentage of pod area covered by LLS) Light leaf spot severity flowering stage;time to BBCH growth stage 62-65;Anthesis Flowering time (BBCH 62-65) Plant Development stage Additional flowering stages according to the BBCH scale (62-65) Phoma incidence Leaf Incidence of Phoma leaf spot The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot Verticilium wilt presence Plant Absence or presence of Verticilium wilt on the plant Verticilium wilt presence Stigmasterol The concentration of Stigmasterol in seeds Stigmasterol content Seed SdStigmasterol Seed Sigmasterol content Visual observations Climatic incidents annotions - Estimation Hail damage Plant damage caused by hail. Hail damage HailDmg Plant Light leaf spot severity Stem LLS severity on stem (percentage of stem area covered by LLS) Light leaf spot severity on stems, judged by the percentage of area covered by LLS. Powdery mildew presence - Estimation Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1 regularity stand - Estimation Visual notations (scale 0 - 9) Image analysis - Computation Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001) Stand Scoring of the regularity of the plant stand Plant Regularity of plant stand NIRS assay. Pct total fatty acids Oleic seed content NIRS - Measurement Avenasterol Avenasterol proportion of Phytosterols Seed The percentage of Avenasterol in the total of Phytosterols in seeds SdAvenasterol_Cp Seed Avenasterol content number pod Pod number Number of pods on terminal raceme Stem witdh - Measurement Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image. SdK The concentration of Potassium in seeds Seed Seed Potassium content Potassium content SdLi The concentration of Lithium in seeds Seed Lithium content Seed Lithium content Plant Oil yield Oil yield OY Oil yield LfRb The concentration of Rubidium in leaves Rubidium content Leaf Leaf Rubidium content Shattering PodShat Proportion of open pods (assessment) Pod shattering Pod Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod width - Measurement SdS Sulphur content Seed The concentration of Sulphur in seeds Seed Sulphur content Biomass yield Yield Weight (kg) of raw materials harvested by plot Biomass Copper content LfCu Leaf Leaf Copper content The concentration of Copper in leaves The concentration of Cadmium in seeds SdCd Cadmium content Seed Cadmium content Seed Cycloartenol content Seed Cycloartenol content SdCycloartenol Seed Cycloartenol The concentration of Cycloartenol in seeds Surface of the elementary experimental plot Size Plot size Plot PlotSize 0-9 Lodging Incidence scale Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation Leaf miner incidence Incidence with leaf miner Leaf The percentage of plants that showed presence of leaf miner from a subsample of a plot GLS seed content NIRS - Measurement NIRS assay Quantity per Seed Dry Weight pod Pod width of pods from terminal raceme Pod width width Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter. Seed sieving - Estimation day-of-year Titanium content Leaf Titanium content The concentration of Titanium in leaves LfTi Leaf kg Presence (1) or absence (0) of powdery mildew Absence or presence of powdery mildew Plant Powdery mildew presence Seed The concentration of Boron in seeds SdB Seed Boron content Boron content Seed Campesterol content Seed Campesterol content Campesterol The concentration of Campesterol in seeds SdCampesterol Seed The concentration of Copper in seeds Seed Copper content Copper content SdCu Alkane-functional group content SdAlk Pod and stem Alkane-functional group content The concentration of components with alkane functional groups from pod and stem material Pod and stem Biotic stress annotations - Estimation Visual observations The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation NIRS assay. Pct total fatty acids Linolenic seed content NIRS - Measurement Seed SdCr Chromium content The concentration of Chromium in seeds Seed Chromium content Ketone functional group content The concentration of components with ketone functional groups from pod and stem material Pod and stem Ketone functional group content SdKeto Pod and stem Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Plot Plot measure validity Assessment on measure validity. Valid Measure validity Erucid seed content NIRS - Measurement NIRS assay. Pct total fatty acids LfZn The concentration of Zinc in leaves Leaf Zinc content Zinc content Leaf Pod Number - Counting Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Development stage Plant BBCH growth stage 31;CETIUM growth stage C1 Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale) First inter-node time (BBCH 31) Length of lower stem from ground level to last formed fertile branch length foot Foot length The concentration of Rubidium in seeds SdRb Seed Seed Rubidium content Rubidium content SD The diameter of a seed Seed diameter Seed diameter Linoleic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Plant Lodging incidence The plant lodging incidence. Lodg Plant lodging incidence Leaf Manganese content The concentration of Manganese in leaves Manganese content Leaf LfMn Seed Cobalt content The concentration of Cobalt in seeds Seed Cobalt content SdCo Oleic acid content Seed Seed oleic acid content The concentration of oleic acid in seeds. StOleic Campesterol diunsat Seed diunsaturated Campesterol content Campesterol diunsat proportion of Phytosterols The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds SdCampesterolDiunsat_Cp Seed Oil content SdOil Seed oil content The concentration of oil in seeds. Seed Seed weight seed Seed weight of ten typical pods weight pod maturity stage;time to BBCH growth stage 82-99 Additional stages for pod maturity according to the BBCH scale (82-99) Development stage Plant Pod maturity time (BBCH 82-99) Seed Nickel content Nickel content Seed SdNi The concentration of Nickel in seeds Verticilium wilt presence - Estimation Scoring of presence or absence of verticilium wilt symptoms across whole plant. Moisture content SdMoist Seed Seed moisture content. Seed moisture content SdAvenasterol Seed Avenasterol content Avenasterol Seed Avenasterol content The concentration of Avenasterol in seeds 2020-10-19T00:00:02 Fertile Branches - Counting Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. dSP Seed Phosphorus content Phosphorus content Seed The concentration of Phosphorus in seeds * Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation. * Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step. * 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples. * Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour. * Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials. * A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions. * The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes. * 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used). * The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane. * Samples are vortexed, centrifuged and the upper hexane phase is removed. * A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first. * Samples are dried in a rotary evaporator or under a stream of nitrogen. * Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID). *GC program: * Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes. * Inlet temp: 325°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement Vanadium content LfV The concentration of Vanadium in leaves Leaf Vanadium content Leaf Leaf The concentration of Selenium in leaves LfSe Leaf Selenium content Selenium content The concentration of Lead in leaves Leaf Lead content Lead content LfPb Leaf The concentration of Potassium in leaves Potassium content LfK Leaf Potassium content Leaf Pod length of pods from terminal raceme pod Pod length length CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod development stages (BBCH 72-79) date – Estimation A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents HPLC (High performance liquid chromatography) analysis - Measurement Leaf miner incidence (diseased) - Counting 10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner. The concentration of Sulphur in leaves Leaf Sulphur content Sulphur content Leaf LfS Humidimeter (sample of seeds) Seed moisture content humidifier - Measurement Oil seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight mm LLS severity on leaf Light Leaf spot severity on leafs Light leaf spot resistance Leaf Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate). Sclerotinia incidence (diseased) - Counting Seed B-Sitosterol content B-Sitosterol proportion of Phytosterols The percentage of B-Sitosterol in the total of Phytosterols in seeds B-Sitosterol SdB-Sitosterol_Cp Seed 0-3 disease stage scale Biotic attack annotation Biotic attack during the crop cycle. Plant BioticPb Exposure to biotic attack; damage du to biotic attack 0-5 percentage bracket scale End of flowering time (BBCH 69) BBCH growth stage 69;end flowering stage Development stage End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale) Plant SdMoist Seed Moisture content Seed moisture content. Seed moisture content LfSr Leaf Strontium content Leaf The concentration of Strontium in leaves Strontium content Addictional flowering stages (BBCH 62-65) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. plants/6 plants LfB The concentration of Boron in leaves Leaf Boron content Leaf Boron content The concentration of components with primary alcohol functional groups from pod and stem material Pod and stemprimary Alkohol functional group content SdAlcI primary Alkohol functional group content Pod and stem Nickel content Leaf Nickel content LfNi The concentration of Nickel in leaves Leaf LfFe Iron content Leaf Iron content Leaf The concentration of Iron in leaves width Stem width stem Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant Plant height - Measurement Height is measured from ground to the top of the plants using a dipstick graduated in cm LLS incidence (diseased) - Estimation 10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%. 10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded Phoma leaf spot incidence (diseased) - Estimation wax content The concentration of wax in the pod and seed material Pod and stem SdTotalWax Pod and stem wax content CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Early pod development (BBCH 73) date – Estimation The mean density of lateral roots along the primary root Lateral root density Lateral root density LRD LLS severity - Estimation 10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated. Number of fertile branches number fertile branch Number of fertile branches Seed Stigmasterol proportion of Phytosterols The percentage of Stigmasterol in the total of Phytosterols in seeds Seed Sigmasterol content Stigmasterol SdStigmasterol_Cp Aluminium content Leaf Aluminium content Leaf LfAl The concentration of Aluminium in leaves Seed The percentage of Brassicasterol in the total of Phytosterols in seeds Seed Brassicasterol content SdBrassicasterol Brassicasterol Brassicasterol proportion of Phytosterols SdCycloartenol_Cp The percentage of Cycloartenol in the total of Phytosterols in seeds Seed Cycloartenol proportion of Phytosterols Seed Cycloartenol content Cycloartenol Pedicel length of pods from terminal raceme Pedicel length length pedicel Length of the lateral root length Lateral root Lateral root length LRL Phoma stem cancer severity Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem canker upper stem lesion score Upper stem Aldehyde functional group content The concentration of components with aldehyde functional groups from pod and stem material Pod and stem Pod and stem Aldehyde functional group content SdAld mg Leaf The concentration of Cadmium in leaves Leaf Cadmium content LfCd Cadmium content CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First inter-node (BBCH 31) date – Estimation Score the damage caused by hail. HailDmg - Estimation Weevil damage - Estimation Observe damage caused by Weevils (all species), and rate the damage. Seed Iron content Seed Iron content The concentration of Iron in seeds SdFe Pod shattering - Estimation Visual notations (scale 0 - 9) Leaf Arsenic content The concentration of Arsenic in leaves Arsenic content Leaf LfAs SdSr Seed Strontium content The concentration of Strontium in seeds Seed Strontium content NIRS assay Seed moisture NIRS - Measurement Seed moisture content – Computation Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively Sclerotinia incidence Plant No of plants with Sclerotinia (out of six) Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. Seed SdStearic Stearic acid content The concentration of stearic acid in seeds. Seed stearic acid content PrY Protein yield Protein yield per ha Seed Seed protein yield SdGLS The concentration of glucosinolates in seeds. Seed Glucosinolate content Seed glucosinolate content Palmitic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod length - Measurement Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively Seed impurity content – Computation Comment Seed SdPalmitic The concentration of palmitic acid in seeds. Seed palmitic acid content Palmitic acid content root Phoma stem cancer severity Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem canker severity Stem μmol/g cm SdMo The concentration of Molybdenum in seeds Seed Molybdenum content Seed Molybdenum content A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester plotharvest - Measurement Development stage BBCH growth stage 81;CETIUM growth stage G5 Plant Beginning of pod maturity time (BBCH 81) 10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale) SdBrassicasterol Brassicasterol content The concentration of Brassicasterol in seeds Brassicasterol Seed Brassicasterol content Seed Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O) Oil yield - Computation Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid” Plot measure validity – Estimation Seed Calcium content Seed Calcium content SdCa The concentration of Calcium in seeds Development stage pod development stage;time to BBCH growth stages 72-79 Pod development time (BBCH 72-79) Plant Additional stages for pod development according to the BBCH scale (72-79) length Primary root PRL Primary root lengh Length of the primary root PltSdEm Seed Plant stand at emergence Percentage of seeds that emerge. Plant emergence Beginning of pod maturity (BBCH 81) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Zinc content The concentration of Zinc in seeds SdZn Seed Seed Zinc content Seed Selenium content SdSe The concentration of Selenium in seeds Seed Selenium content Seed Seed Manganese content SdMn Manganese content The concentration of Manganese in seeds No of plants with Sclerotinia (per plot) Sclerotinia incidence Plant Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. The number of plants that showed presence of phoma leaf spots from a subsample of a plot Plant Number of plants with phoma leaf spots Phoma leaf spots The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Leaf The concentration of Calcium in leaves LfCa Calcium content Leaf Calcium content SdPhytosterol The total concentration of phytosterols in seeds Seed total Phytosterol content Phytosterol content Seed Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr) Seed protein Yield - Computation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First flowers (BBCH 60) date – Estimation Stem length - Measurement Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. 10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated. LLS severity - Estimation BBCH growth stage 60;CETIUM growth stage F1 First flowers time (BBCH 60) First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale) Development stage Plant CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. End of flowering (BBCH 69) date – Estimation LfMg Leaf Magnesium content Leaf Magnesium content The concentration of Magnesium in leaves Phoma stem cancer severity Phoma stem canker base stem score Stem base Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa 10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot. Phoma incidence (diseased) - Counting seed gamma-tocopherol content Gamma- tocopherol conten in seeds Seed gamma- tocopherol content Sdy-toc Evaluation of the Phoma lingam infection with the G2 score. The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation. Phoma damage – G2 score – Computation Erucid acid content Seed erucic acid content SdErucic Seed The concentration of erucic acid in seeds. BBCH growth stage 71;CETIUM growth stage G1 Plant Development stage 10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale) Beginning of pod development time (BBCH 71) pod/20cm of terminal raceme Plant WeevilsDmg Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil). Weevils damage Weevils damage Leaf Phosphorus content Leaf LfP The concentration of Phosphorus in leaves Phosphorus content Number of seeds in ten typical pods Seed number number seed The concentration of Caesium in leaves Caesium content Leaf Caesium content LfCs Leaf Phoma leaf spot severity Leaf Leaf phoma resistance Severity assessment of phoma leaf spot Verticilium wilt presence - Counting Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded. scale_of Plant stand at emergence – Computation EMERG = (Number of plants obtained / Number of seeds sown) x 100 Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Alpha- tocopherol content in seeds seed Sda-toc Seed alpha- tocopherol content alpha-tocopherol content The concentration of linolenic acid in seeds. Linolenic acid content StLinolenic Seed linolenic acid content Seed method_of The concentration of Sodium in seeds Seed Sodium content Seed Sodium content SdNa The concentration of Chromium in leaves Chromium content Leaf Chromium content Leaf LfCr NIRS assay. Pct of Seed Dry Weight Protein seed content NIRS - Measurement Beak length length Pod beak length beak BBCH growth stage 73;CETIUM growth stage G4 Development stage 30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale) Early pod development time (BBCH 73) Plant Sclerotinia incidence (diseased) - Counting The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded. fertile branch Seed B-Sitosterol content Seed B-Sitosterol content SdB-Sitosterol B-Sitosterol The concentration of B-Sitosterol in seeds SdAlcII Pod and stem The concentration of components with secondary alcohol functional groups from pod and stem material secondary Alkohol functional group content Pod and stem secondary Alkohol functional group content CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod maturity stages (BBCH 82-99) date – Estimation Plot Size – Computation Surface is determined by the experimental design SdProt The concentration of proteins in seeds. Seed Seed protein content Protein content Stand Plant density Number of viable plants counted per surface unit Plant PltSd Plant stand Crop stand plant/m2 LLS resistance - Estimation 10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7. CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of flowering (BBCH 61) date - Estimation 0-9 Regularity scale Samples from HI assay are analysed for N_%DW in the different fractions Nitrate Harvest index - Computation plants/plot Crop problems annotations - Estimation Visual observations Leaf phoma resistance - Estimation 10 spots per plant]]> The concentration of Molybdenum in leaves Leaf Molybdenum content LfMo Molybdenum content Leaf CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of pod development (BBCH 71) date – Estimation variable_of Leaf Sodium content LfNa Leaf The concentration of Sodium in leaves Sodium content Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Seed Number - Counting NIRS assay. Pct total fatty acids Stearic seed content NIRS - Measurement The concentration of Balium in leaves Leaf Balium content LfBa Leaf Balium content Pod beak length - Measurement Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant. Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging Plant lodging incidence - Estimation LfAg Leaf Silver content Leaf Silver content The concentration of Silver in leaves