PhomaSKbase_E_0to7 Yongju Huang, John West University of Hertfordshire Nottingham Thomas Alcock, David Salt SdPb_M_mgkg LLSleaf_E_pct Yongju Huang, John West University of Hertfordshire SdStigmasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Martin Broadley,Thomas Alcock LfCa_M_mgkg Nottingham Rachel Wells John Innes Centre PltHght_M_cm stagePodMaturity_cnt_dayj Anne Laperche Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY INRA 1 Trace of disease Nottingham SdSe_M_mgkg Thomas Alcock, David Salt Nottingham SdK_M_mgkg Thomas Alcock, David Salt Martin Broadley,Thomas Alcock LfSe_M_mgkg Nottingham low damage 3 SdStigmasterol_Cp_pct Rothamsted Richard Broughton, Frederic Beaudoin Nottingham SdFe_M_mgkg Thomas Alcock, David Salt Martin Broadley,Thomas Alcock LfCd_M_mgkg Nottingham 3 irregular PhomaLS_Cou_pct Yongju Huang, John West University of Hertfordshire SdAs_M_mgkg Nottingham Thomas Alcock, David Salt Anne Laperche plntHeight_M_cm INRA Plant Size;Height of plants in an experimental plot;PLANTSIZE SdCampesterol diunsat_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted Nottingham LfK_M_mgkg Martin Broadley,Thomas Alcock bioticAnnot_an_text Anne Laperche Biotic Stress;Biotic attack during the crop cycle;BIOTIC INRA Plot Surface;Surface of the elementary experimental plot;PLOTSURF Anne Laperche INRA plotSize_M_m2 Richard Broughton, Frederic Beaudoin SdCycloartenol_Cp_pct Rothamsted Nottingham Martin Broadley,Thomas Alcock LfMg_M_mgkg 6 Anne Laperche Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS INRA seedMoist_mnirs_pct Nottingham Thomas Alcock, David Salt SdZn_M_mgkg Anne Laperche seedMoist_mlab_pct INRA Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB Anne Laperche seedMoistHarv_mhumi_pct INRA Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV SdAvenasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Martin Broadley,Thomas Alcock Nottingham LfAg_M_mgkg Annotations on measure validity;VALIDATION Anne Laperche INRA validateannot_E_cmt Nottingham Thomas Alcock, David Salt SdP_M_mgkg Growth Stage G1;10% of the pods have reached their final size Anne Laperche INRA flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1 stageG1_cnt_dayj Martin Broadley,Thomas Alcock Nottingham LfTi_M_mgkg Rachel Wells FertBraNumb_Ct_Fb John Innes Centre Thomas Alcock, David Salt SdMn_M_mgkg Nottingham Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO stageFlo_cnt_dayj Anne Laperche INRA Anne Laperche INRA Phoma damage G2 score phomaDmg_CompG2_0to9 Richard Broughton, Frederic Beaudoin Rothamsted SdFA_M_mgkg 2-25% girdling 2 SdAvenasterol_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted INRA Growth Stage C1;Bolting date when the first inter-node appears stageC1_cnt_dayj corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1 Anne Laperche Martin Broadley,Thomas Alcock Nottingham LfFe_M_mgkg Anne Laperche Growth Stage F1;First flowers are open stageF1_cnt_dayj corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1 INRA LfZn_M_mgkg Nottingham Martin Broadley,Thomas Alcock 75-100% girdling 5 Nottingham LfBa_M_mgkg Martin Broadley,Thomas Alcock LfCu_M_mgkg Martin Broadley,Thomas Alcock Nottingham Nottingham SdMg_M_mgkg Thomas Alcock, David Salt entity Hail Damage;HAILDMG Anne Laperche hailDmg_E_0to9 INRA Growth Stage G5;10% of the seeds have reached maturity seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5 Anne Laperche INRA stageG5_cnt_dayj LfAs_M_mgkg Martin Broadley,Thomas Alcock Nottingham Rachel Wells SdNum_Ct_sd John Innes Centre 2 Culture leaning, without impact Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC linolenicSeedCont_M_pct INRA Anne Laperche Martin Broadley,Thomas Alcock Nottingham LfPb_M_mgkg LfRb_M_mgkg Martin Broadley,Thomas Alcock Nottingham Richard Broughton, Frederic Beaudoin Rothamsted SdTotalWax_M_mgkg INRA palmiticSeedCont_M_pct Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC Anne Laperche SdNa_M_mgkg Nottingham Thomas Alcock, David Salt John Innes Centre StemWid_M_cm Rachel Wells Nottingham SNi_M_mgkg Thomas Alcock, David Salt INRA Anne Laperche protMealCont_M_pct Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL 1 ≤ 5% girdling Yongju Huang, John West University of Hertfordshire VertWilt_Cou_pct Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI INRA Anne Laperche NHI_Comp_pct PedLen_M_cm Rachel Wells John Innes Centre Yongju Huang, John West University of Hertfordshire white blight Sclerot_Cou_plntPlot Martin Broadley,Thomas Alcock LfB_M_mgkg Nottingham Nottingham Thomas Alcock, David Salt SdCu_M_mgkg PhomaLS_E_0to3 Yongju Huang, John West University of Hertfordshire 3 Prot Yield;Protein yield per ha;PrY Anne Laperche protYield_Comp_qha INRA 9 < 10% University of York Sda-toc_M_mgkg Swen Langer, Lenka Havlickova INRA stearicSeedCont_M_pct Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC Anne Laperche Richard Broughton, Frederic Beaudoin Rothamsted SdAlk_M_mgkg Plant Density;Number of viable plants counted per surface unit;PLANTDENS Anne Laperche INRA plntStnd_M_plntm2 Pod shattering;Scoring of pods open just before harvest;PODSHAT podShat_E_score Anne Laperche INRA PhomaLS_Cou_plnt10plnt Yongju Huang, John West University of Hertfordshire Protein %;Content of proteins in seeds determined by NIRS;Pr protSeedCont_M_pct INRA Anne Laperche Nottingham LfU_M_mgkg Martin Broadley,Thomas Alcock PodNumbTermRac_Ct_PodTermRac Rachel Wells John Innes Centre Nottingham LfMn_M_mgkg Martin Broadley,Thomas Alcock SdMo_M_mgkg Nottingham Thomas Alcock, David Salt Yongju Huang, John West University of Hertfordshire white blight Sclerot_Cou_plnt6plnt VertWilt_E_0or1 Yongju Huang, John West University of Hertfordshire Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC linoleicSeedCont_M_pct Anne Laperche INRA Nottingham SdB_M_mgkg Thomas Alcock, David Salt Anne Laperche INRA corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO stageEndFlo_cnt_dayj Growth Stage End Flowering;End of flowering on the main inflorescence TRL_Cp_cm Nottingham Martin Broadley, Catherine Thomas No disease observable 0 SdBrassicasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Martin Broadley, Catherine Thomas LRL_M_cm Nottingham BeakLen_M_cm John Innes Centre Rachel Wells SdCampesterolDiunsat_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted 1 high > 90% oilYield_Comp_qha Anne Laperche INRA Oil Yield;Oil yield per ha;OY Nottingham LfSr_M_mgkg Martin Broadley,Thomas Alcock no symptoms observable 0 Rothamsted SdCycloartenol_M_mgkg Richard Broughton, Frederic Beaudoin elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2 stageF2_cnt_dayj Anne Laperche Growth Stage F2;10% of the flowers are open on the main inflorescence INRA Thomas Alcock, David Salt Nottingham SdCo_M_mgkg Nottingham Martin Broadley,Thomas Alcock LfAl_M_mgkg 0 No leaf spotting Plants with scattered lesions 3 Nottingham Martin Broadley,Thomas Alcock LfV_M_mgkg Swen Langer, Lenka Havlickova Sdy-toc_M_mgkg University of York acronym 5 Half green/half diseased, leaf curling and distortion Rothamsted SdB-Sitosterol_M_mgkg Richard Broughton, Frederic Beaudoin 7 10-30% Nottingham Martin Broadley, Catherine Thomas MLRL_Cp_cm LLSstem_E_pct Yongju Huang, John West University of Hertfordshire INRA Crop problems;Crop problems during the cycle.;CROP cropAnnot_E_cmt Anne Laperche Nottingham SdCd_M_mgkg Thomas Alcock, David Salt Yongju Huang, John West University of Hertfordshire LLSpods_E_pct Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV Anne Laperche INRA stagePodDev_cnt_dayj <5 spots per plant 1 Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND Anne Laperche regStand_E_0to9 INRA 5 Average loding 4 Diseased leaved with a few large lesions of many small leasions, area quite heavily infected INRA plntStdEmerg_M_plt Plant Emergence;Percentage of seeds that emerge;EMERG Anne Laperche Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC oleicSeedCont_M_pct Anne Laperche INRA Richard Broughton, Frederic Beaudoin SdPhytosterol_M_mgkg Rothamsted Anne Laperche INRA Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC erucicSeedCont_M_pct SdSr_M_mgkg Nottingham Thomas Alcock, David Salt SdAld_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted 4 low to intermediate damage University of Hertfordshire LLSleaf_E_0to7 Yongju Huang, John West 6 Evidence of stunting Martin Broadley,Thomas Alcock LfNa_M_mgkg Nottingham Plant Lodging;Scoring of the plant lodging of the plot;LODGING Anne Laperche INRA lodging_E_0to9 Martin Broadley,Thomas Alcock Nottingham LfCr_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdAlcII_M_mgkg SdBrassicasterol_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted Nottingham LfNi_M_mgkg Martin Broadley,Thomas Alcock 2 Rachel Wells John Innes Centre SdWght_M_mg PhomaSKupper_E_0to7 Yongju Huang, John West University of Hertfordshire oilSeedCont_M_pct Oil %;Content of oil in seeds determined by NIRS;O Anne Laperche INRA Martin Broadley, Catherine Thomas Nottingham LRD_Cp_rootcm trace 1 8 PhomaSK_E_0to7 Yongju Huang, John West University of Hertfordshire Nottingham Martin Broadley,Thomas Alcock LfS_M_mgkg SdCampesterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted 3 >10 spots per plant Nottingham SdS_M_mgkg Thomas Alcock, David Salt SdAlcI_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted 5 moderately regular 9 severe damage 4 Thomas Alcock, David Salt SdLi_M_mgkg Nottingham SdB-Sitosterol_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted Rothamsted SdCampesterol_Cp_pct Richard Broughton, Frederic Beaudoin SdRb_M_mgkg Thomas Alcock, David Salt Nottingham LeafMiner_Cou_pct Yongju Huang, John West University of Hertfordshire 51-75% girdling 4 Rachel Wells John Innes Centre PodLen_M_cm Martin Broadley,Thomas Alcock LfCs_M_mgkg Nottingham 100% girdling ( stem weak/firm) 6 0 NA 0 no visible affection Nottingham Martin Broadley, Catherine Thomas SD_Est_mm No lodging 1 30-60% 5 Martin Broadley, Catherine Thomas LRN_Ct_root Nottingham SdKeto_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Anne Laperche plotHarvest_M_kg INRA Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST INRA Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS glsSeedCont_M_μmolg Anne Laperche Nottingham Martin Broadley,Thomas Alcock LfP_M_mgkg Thomas Alcock, David Salt SdCr_M_mgkg Nottingham SdBranched_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted INRA weevilsDmg_E_0to9 Weevils (all species) damage score;charancon Anne Laperche FtLen_M_cm John Innes Centre Rachel Wells PRL_M_cm Martin Broadley, Catherine Thomas Nottingham Brassica napus ontology Brassica ontology 0 NA intermediate damage 5 8 high to severe damage attribute Yongju Huang, John West University of Hertfordshire PowMildew_E_0or1 Swen Langer, Lenka Havlickova University of York Sdd-toc_M_mgkg INRA climaticAnnot_E_cmt Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC Anne Laperche 7 100% girdling (stem dead/lodged)) INRA Growth Stage G4;30% of the pods have reached their final size stageG4_cnt_dayj flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4 Anne Laperche Diseased leaves with non or a few small lesions 2 7 Plants heavily stunted or dead. high damage 7 2 5-10 spots per plant LfMo_M_mgkg Martin Broadley,Thomas Alcock Nottingham PodWid_M_cm Rachel Wells John Innes Centre SdCa_M_mgkg Nottingham Thomas Alcock, David Salt NA 0 6 intermediate to high damage Anne Laperche INRA Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR seedImpur_M_pct perfectly regular 9 7 regular 2 very low damage 4 60-90% 3 26-50% girdling 3 high irregular 1 SdCycloartenol_Cp The percentage of Cycloartenol in the total of Phytosterols in seeds Cycloartenol proportion of Phytosterols Seed Seed Cycloartenol content Cycloartenol Stem Phoma stem cancer severity Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem canker severity mg/kg Plant Development stage 30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale) BBCH growth stage 73;CETIUM growth stage G4 Early pod development time (BBCH 73) Ketone functional group content Pod and stem Ketone functional group content Pod and stem SdKeto The concentration of components with ketone functional groups from pod and stem material LfU Uranium content The concentration of Uranium in leaves Leaf Uranium content Leaf Seed moisture content Seed Moisture content Seed moisture content. SdMoist Leaf Lead content The concentration of Lead in leaves Leaf LfPb Lead content Seed Strontium content SdSr Strontium content The concentration of Strontium in seeds Seed The concentration of Phosphorus in seeds dSP Phosphorus content Seed Phosphorus content Seed Plant Absence or presence of powdery mildew Presence (1) or absence (0) of powdery mildew Powdery mildew presence Seed Iron content The concentration of Iron in seeds SdFe Iron content Seed BioticPb Biotic attack during the crop cycle. Plant Exposure to biotic attack; damage du to biotic attack Biotic attack annotation seed Seed number Number of seeds in ten typical pods number Phoma stem cancer severity Phoma stem canker base stem score Stem base Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Light leaf spot severity LLS severity on pods (percentage of pod area covered by LLS) Pod Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS. Plant lodging incidence - Estimation Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Addictional flowering stages (BBCH 62-65) date – Estimation Leaf Molybdenum content LfMo Leaf Molybdenum content The concentration of Molybdenum in leaves Sclerotinia incidence (diseased) - Counting The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded. Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Seed weight - Measurement Seed B-Sitosterol content B-Sitosterol The concentration of B-Sitosterol in seeds B-Sitosterol content Seed SdB-Sitosterol The concentration of Lithium in seeds Lithium content Seed Seed Lithium content SdLi Leaf LfK Potassium content Leaf Potassium content The concentration of Potassium in leaves boolean Linoleic acid content StLinoleic Seed Seed linoleic acid content The concentration of linoleic acid in seeds. width pod Pod width of pods from terminal raceme Pod width Powdery mildew presence - Estimation Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1 LfB Leaf Leaf Boron content Boron content The concentration of Boron in leaves Pod maturity time (BBCH 82-99) Development stage Plant Additional stages for pod maturity according to the BBCH scale (82-99) pod maturity stage;time to BBCH growth stage 82-99 Stem witdh - Measurement Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image. SdK Potassium content Seed Potassium content The concentration of Potassium in seeds Seed 10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7. LLS resistance - Estimation Linolenic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Phoma leaf spot incidence (diseased) - Estimation 10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded Leaf LfNa Sodium content Leaf Sodium content The concentration of Sodium in leaves Protein content Seed The concentration of proteins in seeds. Seed protein content SdProt Palmitic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pedicel length - Measurement average length MLRL The mean length of a lateral root of that root system Lateral root Mean lateral root length Erucid seed content NIRS - Measurement NIRS assay. Pct total fatty acids Leaf The percentage of plants that showed presence of leaf miner from a subsample of a plot Leaf miner incidence Incidence with leaf miner Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded. Verticilium wilt presence - Counting The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols Relative seed Phytosterol content - Computation PrY Seed Protein yield Seed protein yield Protein yield per ha Avenasterol SdAvenasterol Avenasterol content The concentration of Avenasterol in seeds Seed Avenasterol content Seed Seed oil content SdOil Seed The concentration of oil in seeds. Oil content Oil seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight Chromium content LfCr Leaf The concentration of Chromium in leaves Leaf Chromium content Leaf LfAg Silver content The concentration of Silver in leaves Leaf Silver content Visual notations (scale 0 - 9) Pod shattering - Estimation SdB-Sitosterol_Cp Seed B-Sitosterol content B-Sitosterol Seed The percentage of B-Sitosterol in the total of Phytosterols in seeds B-Sitosterol proportion of Phytosterols pod/20cm of terminal raceme Leaf Leaf Calcium content LfCa Calcium content The concentration of Calcium in leaves root/cm SdGLS Seed glucosinolate content Glucosinolate content Seed The concentration of glucosinolates in seeds. Maximum plant height from ground to tip of terminal raceme PH plant height Plant height Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Plant height - Measurement Plot measure validity – Estimation Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid” stem Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant Stem width width Early pod development (BBCH 73) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Seed Campesterol SdCampesterol_Cp The percentage of Campesterol in the total of Phytosterols in seeds Campesterol proportion of Phytosterols Seed Campesterol content Phoma leaf spot severity Leaf Leaf phoma resistance Severity assessment of phoma leaf spot length Pedicel length of pods from terminal raceme pedicel Pedicel length scale_of First inter-node (BBCH 31) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Seed SdS Seed Sulphur content The concentration of Sulphur in seeds Sulphur content Development stage End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale) End of flowering time (BBCH 69) BBCH growth stage 69;end flowering stage Plant Plot PlotSize Plot size Size Surface of the elementary experimental plot Manganese content SdMn The concentration of Manganese in seeds Seed Manganese content Seed 2020-10-19T00:00:02 Development stage Plant BBCH growth stage 31;CETIUM growth stage C1 Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale) First inter-node time (BBCH 31) Pod length of pods from terminal raceme pod Pod length length day-of-year Seed SdStigmasterol_Cp The percentage of Stigmasterol in the total of Phytosterols in seeds Seed Sigmasterol content Stigmasterol Stigmasterol proportion of Phytosterols Pod and stemprimary Alkohol functional group content primary Alkohol functional group content Pod and stem The concentration of components with primary alcohol functional groups from pod and stem material SdAlcI The concentration of Zinc in seeds Seed SdZn Seed Zinc content Zinc content 0-9 Lodging Incidence scale The concentration of Calcium in seeds Seed Seed Calcium content Calcium content SdCa * Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation. * Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step. * 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples. * Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour. * Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials. * A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions. * The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes. * 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used). * The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane. * Samples are vortexed, centrifuged and the upper hexane phase is removed. * A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first. * Samples are dried in a rotary evaporator or under a stream of nitrogen. * Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID). *GC program: * Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes. * Inlet temp: 325°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement CropPb Plant Crop problems annotation Crop problems during the crop cycle. Exposure to crop problems;damage from crop problem NIRS assay. Pct total fatty acids Linoleic seed content NIRS - Measurement % The concentration of Copper in seeds Seed Copper content Seed Copper content SdCu 3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg Seed mineral analysis - Measurement 0-9 Damage/Incidence scale Plant Phoma leaf spots Number of plants with phoma leaf spots The number of plants that showed presence of phoma leaf spots from a subsample of a plot Stand Scoring of the regularity of the plant stand Plant Regularity of plant stand Meal protein content MlProt Meal Protein content The concentration of proteins in the meal. cm Seed Seed N content to total plant N content ratio N content proportion of plant N content Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root) NHI Nitrogen Harvest Index PRL Primary root lengh Primary root Length of the primary root length Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation Image analysis - Counting RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN). * 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.). * Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers. * Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C. * Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. * After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS. * GC program: * Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes. * Inlet temp: 280°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement Weevils damage Plant WeevilsDmg Weevils damage Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil). 10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated. LLS severity - Estimation Gamma- tocopherol conten in seeds seed Seed gamma- tocopherol content Sdy-toc gamma-tocopherol content fertile branch A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester plotharvest - Measurement The concentration of all compounds with branched alcyl chains from pod and stem material Pod and stem branched Alcyl-chain content SdBranched Pod and stem branched Alcyl-chain content 0-7 percentage scale Pod and stem wax content The concentration of wax in the pod and seed material Pod and stem SdTotalWax wax content Sda-toc Alpha- tocopherol content in seeds seed alpha-tocopherol content Seed alpha- tocopherol content Seed Cycloartenol Cycloartenol content Seed Cycloartenol content The concentration of Cycloartenol in seeds SdCycloartenol Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation SdBrassicasterol Seed Brassicasterol The percentage of Brassicasterol in the total of Phytosterols in seeds Brassicasterol proportion of Phytosterols Seed Brassicasterol content Height is measured from ground to the top of the plants using a dipstick graduated in cm Plant height - Measurement regularity stand - Estimation Visual notations (scale 0 - 9) SdRb Seed Seed Rubidium content Rubidium content The concentration of Rubidium in seeds NIRS assay. Pct of Seed Dry Weight Protein meal content NIRS - Measurement Length of the lateral root LRL length Lateral root length Lateral root root Evaluation of the Phoma lingam infection with the G2 score. The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation. Phoma damage – G2 score – Computation Pod number pod Number of pods on terminal raceme number The concentration of Iron in leaves Leaf Iron content Iron content LfFe Leaf LfSr The concentration of Strontium in leaves Strontium content Leaf Leaf Strontium content Seed moisture content – Computation Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively Leaf mineral analysis - Measurement 3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry The concentration of Copper in leaves Leaf Copper content Copper content Leaf LfCu Leaf miner incidence (diseased) - Counting 10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner. length Length of all roots Total root length Total root TRL Seed Magnesium content Seed Magnesium content The concentration of Magnesium in seeds SdMg The concentration of Boron in seeds Seed SdB Seed Boron content Boron content Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant. Pod beak length - Measurement SdCampesterolDiunsat_Cp Seed Campesterol diunsat Campesterol diunsat proportion of Phytosterols The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds Seed diunsaturated Campesterol content method_of Leaf Nickel content LfNi Leaf Nickel content The concentration of Nickel in leaves Brassicasterol The concentration of Brassicasterol in seeds Seed Brassicasterol content Brassicasterol content SdBrassicasterol Seed Biomass Yield Biomass yield Weight (kg) of raw materials harvested by plot Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Stem length - Measurement Leaf Leaf Sulphur content LfS The concentration of Sulphur in leaves Sulphur content Arsenic content SdAs The concentration of Arsenic in seeds Seed Arsenic content Seed Leaf Rubidium content The concentration of Rubidium in leaves Rubidium content LfRb Leaf The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Phoma damage Withering disease PhomaDmg Plant Withering disease caused by the agent Leptosphaeria maculans (Phoma). Phoma damage SdMo Molybdenum content Seed Molybdenum content Seed The concentration of Molybdenum in seeds Length of lower stem from ground level to last formed fertile branch foot length Foot length Absence or presence of Verticilium wilt on the plant Verticilium wilt presence Verticilium wilt presence Plant plant/m2 Seed moisture NIRS - Measurement NIRS assay μmol/g Humidimeter (sample of seeds) Seed moisture content humidifier - Measurement Leaf Caesium content Leaf Caesium content The concentration of Caesium in leaves LfCs Seed Cobalt content The concentration of Cobalt in seeds Cobalt content Seed SdCo Seed Sodium content The concentration of Sodium in seeds SdNa Sodium content Seed NIRS assay. Pct total fatty acids Stearic seed content NIRS - Measurement Seed linolenic acid content Linolenic acid content The concentration of linolenic acid in seeds. StLinolenic Seed Plant stand at emergence Plant emergence PltSdEm Seed Percentage of seeds that emerge. SdAld Pod and stem The concentration of components with aldehyde functional groups from pod and stem material Pod and stem Aldehyde functional group content Aldehyde functional group content Plant 10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale) Development stage BBCH growth stage 61;CETIUM growth stage F2 Beginning of flowering time (BBCH 61) mm The concentration of Zinc in leaves Zinc content Leaf Leaf Zinc content LfZn Crop problems annotations - Estimation Visual observations plants/6 plants Leaf Arsenic content The concentration of Arsenic in leaves LfAs Arsenic content Leaf Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) SdCr Chromium content Seed Seed Chromium content The concentration of Chromium in seeds Seed SdCampesterolDiunsat Campesterol diunsat diunsaturated Campesterol content The concentration of diunsaturated Campesterol in seeds Seed diunsaturated Campesterol content Seed weight Seed weight of ten typical pods seed weight Shattering Pod shattering PodShat Proportion of open pods (assessment) Pod Avenasterol proportion of Phytosterols SdAvenasterol_Cp Seed The percentage of Avenasterol in the total of Phytosterols in seeds Seed Avenasterol content Avenasterol Plot Size – Computation Surface is determined by the experimental design LLS severity on stem (percentage of stem area covered by LLS) Light leaf spot severity on stems, judged by the percentage of area covered by LLS. Stem Light leaf spot severity Cadmium content Leaf The concentration of Cadmium in leaves Leaf Cadmium content LfCd Seed SdStigmasterol Stigmasterol content Seed Sigmasterol content The concentration of Stigmasterol in seeds Stigmasterol flowering stage;time to BBCH growth stage 62-65;Anthesis Development stage Plant Additional flowering stages according to the BBCH scale (62-65) Flowering time (BBCH 62-65) SdPalmitic The concentration of palmitic acid in seeds. Seed palmitic acid content Seed Palmitic acid content SdNi The concentration of Nickel in seeds Seed Nickel content Nickel content Seed The concentration of Cadmium in seeds Seed Seed Cadmium content SdCd Cadmium content Leaf Incidence of Phoma leaf spot Phoma incidence The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot No of plants with Sclerotinia (per plot) Sclerotinia incidence Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. Plant Crop stand Number of viable plants counted per surface unit PltSd Plant density Stand Plant Plant stand Seed sieving - Estimation Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter. The concentration of Campesterol in seeds Campesterol content SdCampesterol Seed Seed Campesterol content Campesterol Climatic incidents annotions - Estimation Visual observations Pod and stem Fatty acids functional group content The concentration of components with fatty acids as functional groups from pod and stem material Pod and stem Fatty acids functional group content SdFA OY Oil yield Oil yield Plant Oil yield Leaf Vanadium content Vanadium content Leaf The concentration of Vanadium in leaves LfV The concentration of Phosphorus in leaves Leaf LfP Phosphorus content Leaf Phosphorus content 0-9 Regularity scale Exposure to climatic stress Climatic incidents annotation ClimPb Climatic incidents during the crop cycle. Plant seed Delta- tocopherol content in seeds delta-tocopherol content Seed delta- tocopherol content Sdd-toc Alkane-functional group content Pod and stem Pod and stem Alkane-functional group content The concentration of components with alkane functional groups from pod and stem material SdAlk Seed stearic acid content SdStearic Stearic acid content Seed The concentration of stearic acid in seeds. Image analysis - Computation The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001) Scoring of presence or absence of verticilium wilt symptoms across whole plant. Verticilium wilt presence - Estimation seed Seed Seed erucic acid content Erucid acid content The concentration of erucic acid in seeds. SdErucic Weevil damage - Estimation Observe damage caused by Weevils (all species), and rate the damage. Leaf Manganese content The concentration of Manganese in leaves Manganese content LfMn Leaf Seed moisture content. Seed moisture content Moisture content Seed SdMoist Impurities content SdImpur Seed impurity content Percentage of impurities in a sample of seeds determined by weighing. Seed LfAl Leaf The concentration of Aluminium in leaves Aluminium content Leaf Aluminium content A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents HPLC (High performance liquid chromatography) analysis - Measurement q/ha plants/10 plants 10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot. Phoma incidence (diseased) - Counting Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Beginning of pod development time (BBCH 71) BBCH growth stage 71;CETIUM growth stage G1 Plant 10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale) Development stage Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod width - Measurement LLS severity - Estimation 10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated. 0-3 disease stage scale Leaf Magnesium content Leaf LfMg The concentration of Magnesium in leaves Magnesium content Seed impurity content – Computation Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively Oleic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Pod length - Measurement Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Verticilium wilt presence The percentage of plants that showed presence of leaf miner from a subsample of a plot Plant Verticillium wilt incidence Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Fertile Branches - Counting CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of pod maturity (BBCH 81) date – Estimation BBCH growth stage 81;CETIUM growth stage G5 Plant Development stage Beginning of pod maturity time (BBCH 81) 10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale) Seed Seed diameter diameter SD The diameter of a seed Number of fertile branches number fertile branch Number of fertile branches End of flowering (BBCH 69) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. SdPhytosterol The total concentration of phytosterols in seeds Seed total Phytosterol content Phytosterol content Seed Development stage Pod development time (BBCH 72-79) pod development stage;time to BBCH growth stages 72-79 Plant Additional stages for pod development according to the BBCH scale (72-79) NIRS assay. Pct of Seed Dry Weight Protein seed content NIRS - Measurement Samples from HI assay are analysed for N_%DW in the different fractions Nitrate Harvest index - Computation Seed protein Yield - Computation Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr) NIRS assay Quantity per Seed Dry Weight GLS seed content NIRS - Measurement 0-5 percentage bracket scale Count the number of plants on linear sections defined in each sowing line at the exception of the border lines Plant stand - Counting CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First flowers (BBCH 60) date – Estimation Additionnal pod development stages (BBCH 72-79) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. LLS incidence on leaf Light leaf spot incidence The percentage of plants that showed presence of light leaf spot incidence on leaves Leaf 10 spots per plant]]> Leaf phoma resistance - Estimation Lateral root LRN number Lateral root number Number of lateral roots CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of pod development (BBCH 71) date – Estimation Light leaf spot resistance LLS severity on leaf Light Leaf spot severity on leafs Leaf mg Plant damage caused by hail. HailDmg Hail damage Plant Hail damage Comment LfBa The concentration of Balium in leaves Balium content Leaf Leaf Balium content SdAlcII Pod and stem secondary Alkohol functional group content The concentration of components with secondary alcohol functional groups from pod and stem material secondary Alkohol functional group content Pod and stem First flowers time (BBCH 60) First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale) Development stage Plant BBCH growth stage 60;CETIUM growth stage F1 The concentration of Selenium in leaves Leaf Selenium content LfSe Leaf Selenium content Beak length length beak Pod beak length CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of flowering (BBCH 61) date - Estimation No of plants with Sclerotinia (out of six) Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. Plant Sclerotinia incidence Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O) Oil yield - Computation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod maturity stages (BBCH 82-99) date – Estimation variable_of kg The concentration of oleic acid in seeds. Oleic acid content Seed Seed oleic acid content StOleic Seed Number - Counting Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Assessment on measure validity. Measure validity Plot measure validity Plot Valid Score the damage caused by hail. HailDmg - Estimation Selenium content The concentration of Selenium in seeds SdSe Seed Seed Selenium content LfTi Leaf Titanium content Titanium content The concentration of Titanium in leaves Leaf Lead content Seed Lead content SdPb Seed The concentration of Lead in seeds 0-7 disease stage scale Phoma stem canker upper stem lesion score Upper stem Phoma stem cancer severity Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate). Sclerotinia incidence (diseased) - Counting density Lateral root LRD The mean density of lateral roots along the primary root Lateral root density Plant stand at emergence – Computation EMERG = (Number of plants obtained / Number of seeds sown) x 100 Biotic stress annotations - Estimation Visual observations 10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%. LLS incidence (diseased) - Estimation Pod Number - Counting Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Lodg Plant Plant lodging incidence The plant lodging incidence. Lodging incidence plants/plot