Brassica napus ontology Brassica ontology SdB-Sitosterol_Cp_pct Rothamsted Richard Broughton, Frederic Beaudoin Nottingham Thomas Alcock, David Salt SdCu_M_mgkg SdSe_M_mgkg Nottingham Thomas Alcock, David Salt 4 no visible affection 0 Nottingham Martin Broadley,Thomas Alcock LfBa_M_mgkg SdWght_M_mg John Innes Centre Rachel Wells SNi_M_mgkg Nottingham Thomas Alcock, David Salt stearicSeedCont_M_pct Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC Anne Laperche INRA SdAlcI_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Richard Broughton, Frederic Beaudoin Rothamsted SdAld_M_mgkg stagePodDev_cnt_dayj Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV INRA Anne Laperche very low damage 2 LfAs_M_mgkg Nottingham Martin Broadley,Thomas Alcock INRA Anne Laperche oleicSeedCont_M_pct Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC INRA oilYield_Comp_qha Anne Laperche Oil Yield;Oil yield per ha;OY LfNa_M_mgkg Nottingham Martin Broadley,Thomas Alcock Richard Broughton, Frederic Beaudoin SdBrassicasterol_M_mgkg Rothamsted Nottingham LfAg_M_mgkg Martin Broadley,Thomas Alcock podShat_E_score INRA Anne Laperche Pod shattering;Scoring of pods open just before harvest;PODSHAT University of Hertfordshire PhomaLS_Cou_pct Yongju Huang, John West Nottingham Martin Broadley,Thomas Alcock LfU_M_mgkg SdCampesterol diunsat_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted Anne Laperche stageFlo_cnt_dayj INRA Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO Anne Laperche INRA stageF2_cnt_dayj elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2 Growth Stage F2;10% of the flowers are open on the main inflorescence LfNi_M_mgkg Nottingham Martin Broadley,Thomas Alcock 5-10 spots per plant 2 Rothamsted SdFA_M_mgkg Richard Broughton, Frederic Beaudoin Nottingham Martin Broadley,Thomas Alcock LfP_M_mgkg Anne Laperche INRA climaticAnnot_E_cmt Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC erucicSeedCont_M_pct INRA Anne Laperche Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4 INRA Anne Laperche stageG4_cnt_dayj Growth Stage G4;30% of the pods have reached their final size John Innes Centre Rachel Wells PodWid_M_cm Yongju Huang, John West Sclerot_Cou_plnt6plnt University of Hertfordshire white blight seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5 stageG5_cnt_dayj Anne Laperche Growth Stage G5;10% of the seeds have reached maturity INRA NHI_Comp_pct INRA Anne Laperche Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI seedMoistHarv_mhumi_pct Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV INRA Anne Laperche SdCampesterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Martin Broadley,Thomas Alcock Nottingham LfS_M_mgkg Martin Broadley,Thomas Alcock Nottingham LfCd_M_mgkg Martin Broadley, Catherine Thomas LRN_Ct_root Nottingham lodging_E_0to9 INRA Plant Lodging;Scoring of the plant lodging of the plot;LODGING Anne Laperche hailDmg_E_0to9 Hail Damage;HAILDMG INRA Anne Laperche Martin Broadley, Catherine Thomas PRL_M_cm Nottingham Richard Broughton, Frederic Beaudoin SdB-Sitosterol_M_mgkg Rothamsted Thomas Alcock, David Salt SdPb_M_mgkg Nottingham Thomas Alcock, David Salt Nottingham SdNa_M_mgkg palmiticSeedCont_M_pct Anne Laperche Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC INRA Thomas Alcock, David Salt Nottingham SdCd_M_mgkg 7 Plants heavily stunted or dead. FtLen_M_cm John Innes Centre Rachel Wells SdCr_M_mgkg Nottingham Thomas Alcock, David Salt University of Hertfordshire LLSleaf_E_pct Yongju Huang, John West SdCa_M_mgkg Nottingham Thomas Alcock, David Salt INRA Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY Anne Laperche stagePodMaturity_cnt_dayj INRA Anne Laperche stageEndFlo_cnt_dayj corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO Growth Stage End Flowering;End of flowering on the main inflorescence 26-50% girdling 3 INRA Anne Laperche Weevils (all species) damage score;charancon weevilsDmg_E_0to9 Thomas Alcock, David Salt Nottingham SdMg_M_mgkg Martin Broadley,Thomas Alcock Nottingham LfRb_M_mgkg Plant Size;Height of plants in an experimental plot;PLANTSIZE plntHeight_M_cm INRA Anne Laperche FertBraNumb_Ct_Fb John Innes Centre Rachel Wells University of York Swen Langer, Lenka Havlickova Sdd-toc_M_mgkg regular 7 Nottingham MLRL_Cp_cm Martin Broadley, Catherine Thomas University of York Swen Langer, Lenka Havlickova Sdy-toc_M_mgkg Richard Broughton, Frederic Beaudoin SdCampesterol_Cp_pct Rothamsted Thomas Alcock, David Salt Nottingham SdK_M_mgkg intermediate damage 5 seedImpur_M_pct INRA Anne Laperche Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR Martin Broadley, Catherine Thomas SD_Est_mm Nottingham Yongju Huang, John West VertWilt_Cou_pct University of Hertfordshire SdLi_M_mgkg Nottingham Thomas Alcock, David Salt Anne Laperche INRA Oil %;Content of oil in seeds determined by NIRS;O oilSeedCont_M_pct moderately regular 5 Richard Broughton, Frederic Beaudoin SdBrassicasterol_Cp_pct Rothamsted SdSr_M_mgkg Thomas Alcock, David Salt Nottingham low damage 3 LfSr_M_mgkg Martin Broadley,Thomas Alcock Nottingham INRA Anne Laperche phomaDmg_CompG2_0to9 Phoma damage G2 score Yongju Huang, John West LLSstem_E_pct University of Hertfordshire 51-75% girdling 4 100% girdling ( stem weak/firm) 6 SdZn_M_mgkg Nottingham Thomas Alcock, David Salt LLSleaf_E_0to7 Yongju Huang, John West University of Hertfordshire protSeedCont_M_pct Anne Laperche INRA Protein %;Content of proteins in seeds determined by NIRS;Pr Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS seedMoist_mnirs_pct Anne Laperche INRA Nottingham Thomas Alcock, David Salt SdFe_M_mgkg SdCo_M_mgkg Nottingham Thomas Alcock, David Salt LfCa_M_mgkg Martin Broadley,Thomas Alcock Nottingham Rothamsted SdKeto_M_mgkg Richard Broughton, Frederic Beaudoin LfSe_M_mgkg Nottingham Martin Broadley,Thomas Alcock INRA Anne Laperche plntStdEmerg_M_plt Plant Emergence;Percentage of seeds that emerge;EMERG acronym University of Hertfordshire PhomaSK_E_0to7 Yongju Huang, John West Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST plotHarvest_M_kg INRA Anne Laperche University of Hertfordshire PhomaLS_Cou_plnt10plnt Yongju Huang, John West 10-30% 7 LfCr_M_mgkg Nottingham Martin Broadley,Thomas Alcock University of Hertfordshire LeafMiner_Cou_pct Yongju Huang, John West INRA Anne Laperche Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL protMealCont_M_pct Nottingham Martin Broadley,Thomas Alcock LfPb_M_mgkg Nottingham Thomas Alcock, David Salt SdMo_M_mgkg LfZn_M_mgkg Nottingham Martin Broadley,Thomas Alcock Anne Laperche INRA Prot Yield;Protein yield per ha;PrY protYield_Comp_qha University of Hertfordshire PhomaSKbase_E_0to7 Yongju Huang, John West SdAs_M_mgkg Nottingham Thomas Alcock, David Salt >10 spots per plant 3 Nottingham LRL_M_cm Martin Broadley, Catherine Thomas Half green/half diseased, leaf curling and distortion 5 Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND Anne Laperche INRA regStand_E_0to9 Richard Broughton, Frederic Beaudoin Rothamsted SdAlcII_M_mgkg PltHght_M_cm John Innes Centre Rachel Wells Nottingham Martin Broadley,Thomas Alcock LfTi_M_mgkg LfB_M_mgkg Nottingham Martin Broadley,Thomas Alcock Sda-toc_M_mgkg University of York Swen Langer, Lenka Havlickova Annotations on measure validity;VALIDATION validateannot_E_cmt INRA Anne Laperche SdP_M_mgkg Thomas Alcock, David Salt Nottingham INRA Anne Laperche Plot Surface;Surface of the elementary experimental plot;PLOTSURF plotSize_M_m2 Rothamsted SdBranched_M_mgkg Richard Broughton, Frederic Beaudoin SdCampesterolDiunsat_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin 0 NA Martin Broadley,Thomas Alcock LfMg_M_mgkg Nottingham SdStigmasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted LfK_M_mgkg Nottingham Martin Broadley,Thomas Alcock John Innes Centre PodLen_M_cm Rachel Wells 1 trace INRA stageG1_cnt_dayj flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1 Anne Laperche Growth Stage G1;10% of the pods have reached their final size John Innes Centre Rachel Wells StemWid_M_cm 3 irregular LfFe_M_mgkg Nottingham Martin Broadley,Thomas Alcock Plants with scattered lesions 3 LfV_M_mgkg Martin Broadley,Thomas Alcock Nottingham 5 75-100% girdling Thomas Alcock, David Salt Nottingham SdB_M_mgkg INRA Anne Laperche Biotic Stress;Biotic attack during the crop cycle;BIOTIC bioticAnnot_an_text 1 high > 90% LRD_Cp_rootcm Nottingham Martin Broadley, Catherine Thomas Rothamsted Richard Broughton, Frederic Beaudoin SdCycloartenol_Cp_pct LfCu_M_mgkg Martin Broadley,Thomas Alcock Nottingham No lodging 1 glsSeedCont_M_μmolg INRA Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS Anne Laperche corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1 stageF1_cnt_dayj INRA Anne Laperche Growth Stage F1;First flowers are open University of Hertfordshire PhomaSKupper_E_0to7 Yongju Huang, John West University of Hertfordshire VertWilt_E_0or1 Yongju Huang, John West cropAnnot_E_cmt INRA Anne Laperche Crop problems;Crop problems during the cycle.;CROP Trace of disease 1 7 high damage SdNum_Ct_sd John Innes Centre Rachel Wells BeakLen_M_cm John Innes Centre Rachel Wells Thomas Alcock, David Salt Nottingham SdMn_M_mgkg 8 Martin Broadley, Catherine Thomas TRL_Cp_cm Nottingham PedLen_M_cm John Innes Centre Rachel Wells Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC Anne Laperche linolenicSeedCont_M_pct INRA Yongju Huang, John West University of Hertfordshire white blight Sclerot_Cou_plntPlot Richard Broughton, Frederic Beaudoin SdAvenasterol_Cp_pct Rothamsted SdCycloartenol_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin INRA Anne Laperche seedMoist_mlab_pct Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB Diseased leaves with non or a few small lesions 2 0 NA Martin Broadley,Thomas Alcock LfAl_M_mgkg Nottingham 2 Culture leaning, without impact Richard Broughton, Frederic Beaudoin SdStigmasterol_Cp_pct Rothamsted Yongju Huang, John West LLSpods_E_pct University of Hertfordshire LfMn_M_mgkg Nottingham Martin Broadley,Thomas Alcock SdAlk_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin LfCs_M_mgkg Nottingham Martin Broadley,Thomas Alcock John Innes Centre Rachel Wells PodNumbTermRac_Ct_PodTermRac University of Hertfordshire PhomaLS_E_0to3 Yongju Huang, John West no symptoms observable 0 SdAvenasterol_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin Martin Broadley,Thomas Alcock Nottingham LfMo_M_mgkg Richard Broughton, Frederic Beaudoin SdPhytosterol_M_mgkg Rothamsted 8 high to severe damage 6 SdS_M_mgkg Nottingham Thomas Alcock, David Salt plntStnd_M_plntm2 Anne Laperche Plant Density;Number of viable plants counted per surface unit;PLANTDENS INRA INRA Anne Laperche Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC linoleicSeedCont_M_pct 3 60-90% 2 attribute 9 < 10% low to intermediate damage 4 Rothamsted Richard Broughton, Frederic Beaudoin SdTotalWax_M_mgkg 7 100% girdling (stem dead/lodged)) Thomas Alcock, David Salt SdRb_M_mgkg Nottingham corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1 stageC1_cnt_dayj Growth Stage C1;Bolting date when the first inter-node appears INRA Anne Laperche <5 spots per plant 1 University of Hertfordshire PowMildew_E_0or1 Yongju Huang, John West 1 high irregular 9 severe damage entity 2-25% girdling 2 9 perfectly regular NA 0 No leaf spotting 0 Diseased leaved with a few large lesions of many small leasions, area quite heavily infected 4 6 intermediate to high damage ≤ 5% girdling 1 No disease observable 0 Average loding 5 3 4 5 30-60% Evidence of stunting 6 width Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant stem Stem width The concentration of Iron in leaves Leaf Iron content LfFe Leaf Iron content Chromium content LfCr Leaf Chromium content Leaf The concentration of Chromium in leaves mg/kg Pedicel length length pedicel Pedicel length of pods from terminal raceme Count the number of plants on linear sections defined in each sowing line at the exception of the border lines Plant stand - Counting fertile branch SdStigmasterol_Cp Stigmasterol proportion of Phytosterols Seed Sigmasterol content Stigmasterol Seed The percentage of Stigmasterol in the total of Phytosterols in seeds Cadmium content Seed Cadmium content The concentration of Cadmium in seeds SdCd Seed A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester plotharvest - Measurement SdOil Seed Seed oil content Oil content The concentration of oil in seeds. Seed Molybdenum content The concentration of Molybdenum in seeds Seed Molybdenum content SdMo Pod and stem secondary Alkohol functional group content Pod and stem SdAlcII The concentration of components with secondary alcohol functional groups from pod and stem material secondary Alkohol functional group content Leaf Leaf Uranium content Uranium content The concentration of Uranium in leaves LfU Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Seed sieving - Estimation Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter. SdMoist Seed moisture content Moisture content Seed Seed moisture content. plant height PH Maximum plant height from ground to tip of terminal raceme Plant height The concentration of Magnesium in leaves LfMg Leaf Magnesium content Magnesium content Leaf 10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot. Phoma incidence (diseased) - Counting Seed Iron content SdFe The concentration of Iron in seeds Iron content Seed Plant height - Measurement Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Cycloartenol content The concentration of Cycloartenol in seeds Seed Cycloartenol content Cycloartenol SdCycloartenol Seed Meal protein content The concentration of proteins in the meal. Protein content MlProt Meal * 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.). * Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers. * Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C. * Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. * After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS. * GC program: * Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes. * Inlet temp: 280°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement Stigmasterol content Seed Sigmasterol content Stigmasterol The concentration of Stigmasterol in seeds SdStigmasterol Seed Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Pod Number - Counting mm % CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of flowering (BBCH 61) date - Estimation Oil yield Oil yield OY Plant Oil yield HailDmg Hail damage Hail damage Plant Plant damage caused by hail. The concentration of Sodium in leaves Sodium content Leaf Sodium content Leaf LfNa BBCH growth stage 60;CETIUM growth stage F1 First flowers time (BBCH 60) First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale) Development stage Plant kg Plant stand at emergence Seed Percentage of seeds that emerge. PltSdEm Plant emergence Beginning of pod maturity (BBCH 81) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Leaf miner incidence (diseased) - Counting 10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner. Relative seed Phytosterol content - Computation The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols gamma-tocopherol content Gamma- tocopherol conten in seeds Sdy-toc seed Seed gamma- tocopherol content Seed number number Number of seeds in ten typical pods seed Leaf mineral analysis - Measurement 3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry fertile branch Number of fertile branches number Number of fertile branches Seed Potassium content SdK The concentration of Potassium in seeds Seed Potassium content LfCd Leaf Cadmium content The concentration of Cadmium in leaves Leaf Cadmium content LfTi Leaf Titanium content Leaf Titanium content The concentration of Titanium in leaves BBCH growth stage 81;CETIUM growth stage G5 10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale) Development stage Plant Beginning of pod maturity time (BBCH 81) Pedicel length - Measurement Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Score the damage caused by hail. HailDmg - Estimation MLRL average length Lateral root The mean length of a lateral root of that root system Mean lateral root length SdBrassicasterol Seed Seed Brassicasterol content The percentage of Brassicasterol in the total of Phytosterols in seeds Brassicasterol Brassicasterol proportion of Phytosterols Seed mineral analysis - Measurement 3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg SdPb Seed Lead content Lead content Seed The concentration of Lead in seeds Weight (kg) of raw materials harvested by plot Biomass yield Biomass Yield Seed Sulphur content Sulphur content SdS Seed The concentration of Sulphur in seeds CropPb Plant Crop problems during the crop cycle. Crop problems annotation Exposure to crop problems;damage from crop problem Seed SdPalmitic Palmitic acid content Seed palmitic acid content The concentration of palmitic acid in seeds. 0-9 Regularity scale LfB Leaf The concentration of Boron in leaves Boron content Leaf Boron content SdProt Seed The concentration of proteins in seeds. Protein content Seed protein content Light leaf spot resistance Leaf LLS severity on leaf Light Leaf spot severity on leafs Surface is determined by the experimental design Plot Size – Computation Primary root lengh Length of the primary root Primary root length PRL SdNi Nickel content The concentration of Nickel in seeds Seed Seed Nickel content Sclerotinia incidence Plant No of plants with Sclerotinia (per plot) Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. Selenium content LfSe Leaf The concentration of Selenium in leaves Leaf Selenium content The concentration of linoleic acid in seeds. StLinoleic Seed Linoleic acid content Seed linoleic acid content 0-7 percentage scale CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod development stages (BBCH 72-79) date – Estimation Magnesium content Seed Magnesium content SdMg The concentration of Magnesium in seeds Seed Crop problems annotations - Estimation Visual observations Leaf Leaf Sulphur content Sulphur content The concentration of Sulphur in leaves LfS Seed Seed glucosinolate content SdGLS The concentration of glucosinolates in seeds. Glucosinolate content PodShat Pod Proportion of open pods (assessment) Shattering Pod shattering Strontium content SdSr The concentration of Strontium in seeds Seed Seed Strontium content Leaf Strontium content Leaf Strontium content The concentration of Strontium in leaves LfSr Lateral root number LRN Lateral root Number of lateral roots number day-of-year CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod maturity stages (BBCH 82-99) date – Estimation Seed Manganese content Manganese content Seed The concentration of Manganese in seeds SdMn Campesterol Seed Seed Campesterol content SdCampesterol Campesterol content The concentration of Campesterol in seeds Early pod development time (BBCH 73) 30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale) BBCH growth stage 73;CETIUM growth stage G4 Development stage Plant Plant lodging incidence - Estimation Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate). Sclerotinia incidence (diseased) - Counting Pod width of pods from terminal raceme pod width Pod width Seed Lithium content Seed Lithium content SdLi The concentration of Lithium in seeds Leaf Manganese content The concentration of Manganese in leaves LfMn Leaf Manganese content LfCu Leaf Copper content Leaf The concentration of Copper in leaves Copper content Alkane-functional group content Pod and stem Alkane-functional group content Pod and stem The concentration of components with alkane functional groups from pod and stem material SdAlk B-Sitosterol Seed The percentage of B-Sitosterol in the total of Phytosterols in seeds B-Sitosterol proportion of Phytosterols Seed B-Sitosterol content SdB-Sitosterol_Cp 0-7 disease stage scale Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Seed linolenic acid content Seed The concentration of linolenic acid in seeds. Linolenic acid content StLinolenic Visual notations (scale 0 - 9) Pod shattering - Estimation 0-5 percentage bracket scale Humidimeter (sample of seeds) Seed moisture content humidifier - Measurement Comment RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN). Image analysis - Counting Oil seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight NIRS assay Seed moisture NIRS - Measurement seed Sdd-toc Seed delta- tocopherol content Delta- tocopherol content in seeds delta-tocopherol content PhomaDmg Phoma damage Withering disease caused by the agent Leptosphaeria maculans (Phoma). Phoma damage Plant Withering disease NIRS assay. Pct total fatty acids Erucid seed content NIRS - Measurement Seed Oleic acid content StOleic Seed oleic acid content The concentration of oleic acid in seeds. Pod and stem SdAlcI The concentration of components with primary alcohol functional groups from pod and stem material primary Alkohol functional group content Pod and stemprimary Alkohol functional group content Cycloartenol SdCycloartenol_Cp Cycloartenol proportion of Phytosterols The percentage of Cycloartenol in the total of Phytosterols in seeds Seed Seed Cycloartenol content Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr) Seed protein Yield - Computation The concentration of components with ketone functional groups from pod and stem material Pod and stem SdKeto Pod and stem Ketone functional group content Ketone functional group content SD Seed The diameter of a seed Seed diameter diameter Plot Assessment on measure validity. Plot measure validity Measure validity Valid The concentration of Avenasterol in seeds Seed Avenasterol content Avenasterol Seed Avenasterol content SdAvenasterol length Total root TRL Length of all roots Total root length seed LLS severity - Estimation 10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated. LLS incidence on leaf The percentage of plants that showed presence of light leaf spot incidence on leaves Light leaf spot incidence Leaf Nickel content LfNi The concentration of Nickel in leaves Leaf Nickel content Leaf Weevils damage Plant Weevils damage Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil). WeevilsDmg NIRS assay. Pct total fatty acids Oleic seed content NIRS - Measurement Light leaf spot severity Pod Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS. LLS severity on pods (percentage of pod area covered by LLS) The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Addictional flowering stages (BBCH 62-65) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. pod/20cm of terminal raceme Alpha- tocopherol content in seeds Sda-toc Seed alpha- tocopherol content alpha-tocopherol content seed Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root) Seed N content to total plant N content ratio Seed N content proportion of plant N content NHI Nitrogen Harvest Index The concentration of B-Sitosterol in seeds Seed B-Sitosterol content B-Sitosterol Seed B-Sitosterol content SdB-Sitosterol Pod length of pods from terminal raceme pod Pod length length PrY Seed Protein yield Protein yield per ha Seed protein yield Leaf LfZn The concentration of Zinc in leaves Leaf Zinc content Zinc content Incidence of Phoma leaf spot Leaf The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot Phoma incidence NIRS assay Quantity per Seed Dry Weight GLS seed content NIRS - Measurement First inter-node time (BBCH 31) Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale) Development stage Plant BBCH growth stage 31;CETIUM growth stage C1 Image analysis - Computation Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001) cm SdCu Seed The concentration of Copper in seeds Copper content Seed Copper content CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First inter-node (BBCH 31) date – Estimation The concentration of Rubidium in seeds Seed Rubidium content Rubidium content Seed SdRb Campesterol diunsat Seed Campesterol diunsat proportion of Phytosterols Seed diunsaturated Campesterol content The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds SdCampesterolDiunsat_Cp Seed Calcium content SdCa Seed Calcium content The concentration of Calcium in seeds variable_of * Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation. * Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step. * 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples. * Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour. * Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials. * A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions. * The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes. * 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used). * The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane. * Samples are vortexed, centrifuged and the upper hexane phase is removed. * A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first. * Samples are dried in a rotary evaporator or under a stream of nitrogen. * Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID). *GC program: * Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes. * Inlet temp: 325°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod width - Measurement dSP Phosphorus content The concentration of Phosphorus in seeds Seed Seed Phosphorus content Leaf Leaf miner incidence Incidence with leaf miner The percentage of plants that showed presence of leaf miner from a subsample of a plot Size PlotSize Surface of the elementary experimental plot Plot size Plot Early pod development (BBCH 73) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. The concentration of Balium in leaves LfBa Leaf Leaf Balium content Balium content Leaf Leaf Phosphorus content LfP Phosphorus content The concentration of Phosphorus in leaves The percentage of plants that showed presence of leaf miner from a subsample of a plot Verticillium wilt incidence Verticilium wilt presence Plant 10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded Phoma leaf spot incidence (diseased) - Estimation Number of plants with phoma leaf spots The number of plants that showed presence of phoma leaf spots from a subsample of a plot Plant Phoma leaf spots No of plants with Sclerotinia (out of six) Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. Plant Sclerotinia incidence 0-9 Damage/Incidence scale Seed erucic acid content SdErucic The concentration of erucic acid in seeds. Erucid acid content Seed 10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%. LLS incidence (diseased) - Estimation Seed Cobalt content Cobalt content SdCo Seed The concentration of Cobalt in seeds Plant Additional flowering stages according to the BBCH scale (62-65) flowering stage;time to BBCH growth stage 62-65;Anthesis Flowering time (BBCH 62-65) Development stage Exposure to climatic stress ClimPb Climatic incidents annotation Plant Climatic incidents during the crop cycle. Aluminium content The concentration of Aluminium in leaves Leaf Aluminium content LfAl Leaf 10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale) Development stage Beginning of pod development time (BBCH 71) Plant BBCH growth stage 71;CETIUM growth stage G1 The total concentration of phytosterols in seeds SdPhytosterol Seed Phytosterol content Seed total Phytosterol content Seed moisture content Seed moisture content. Seed SdMoist Moisture content Protein seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight Verticilium wilt presence - Counting Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded. Campesterol SdCampesterol_Cp Seed Seed Campesterol content Campesterol proportion of Phytosterols The percentage of Campesterol in the total of Phytosterols in seeds NIRS assay. Pct of Seed Dry Weight Protein meal content NIRS - Measurement Pod length - Measurement Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Seed Selenium content SdSe The concentration of Selenium in seeds Selenium content Seed 10 spots per plant]]> Leaf phoma resistance - Estimation Leaf The concentration of Caesium in leaves Leaf Caesium content Caesium content LfCs Scoring of presence or absence of verticilium wilt symptoms across whole plant. Verticilium wilt presence - Estimation Length of the lateral root Lateral root length LRL Lateral root length Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant. Pod beak length - Measurement HPLC (High performance liquid chromatography) analysis - Measurement A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents SdB Seed Boron content Seed Boron content The concentration of Boron in seeds LLS severity on stem (percentage of stem area covered by LLS) Stem Light leaf spot severity on stems, judged by the percentage of area covered by LLS. Light leaf spot severity Leaf LfV Leaf Vanadium content Vanadium content The concentration of Vanadium in leaves Rubidium content Leaf Rubidium content Leaf LfRb The concentration of Rubidium in leaves Stand Number of viable plants counted per surface unit Crop stand PltSd Plant density Plant Plant stand Seed weight seed weight Seed weight of ten typical pods boolean Fertile Branches - Counting Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Lateral root density The mean density of lateral roots along the primary root Lateral root LRD density The concentration of diunsaturated Campesterol in seeds diunsaturated Campesterol content Seed diunsaturated Campesterol content SdCampesterolDiunsat Seed Campesterol diunsat Leaf LfPb Lead content Leaf Lead content The concentration of Lead in leaves SdBrassicasterol Seed Brassicasterol Brassicasterol content Seed Brassicasterol content The concentration of Brassicasterol in seeds Nitrate Harvest index - Computation Samples from HI assay are analysed for N_%DW in the different fractions Leaf phoma resistance Leaf Severity assessment of phoma leaf spot Phoma leaf spot severity Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image. Stem witdh - Measurement Phoma damage – G2 score – Computation Evaluation of the Phoma lingam infection with the G2 score. The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation. Leaf Molybdenum content Leaf Molybdenum content LfMo The concentration of Molybdenum in leaves Leaf Arsenic content LfAs The concentration of Arsenic in leaves Arsenic content Leaf pod development stage;time to BBCH growth stages 72-79 Development stage Plant Additional stages for pod development according to the BBCH scale (72-79) Pod development time (BBCH 72-79) Seed Number - Counting Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Plant height - Measurement Height is measured from ground to the top of the plants using a dipstick graduated in cm Visual observations Biotic stress annotations - Estimation EMERG = (Number of plants obtained / Number of seeds sown) x 100 Plant stand at emergence – Computation Seed moisture content – Computation Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively Climatic incidents annotions - Estimation Visual observations The concentration of Arsenic in seeds Seed Arsenic content Seed SdAs Arsenic content μmol/g Linolenic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Palmitic seed content NIRS - Measurement NIRS assay. Pct total fatty acids q/ha Pod maturity time (BBCH 82-99) Plant pod maturity stage;time to BBCH growth stage 82-99 Additional stages for pod maturity according to the BBCH scale (82-99) Development stage Biotic attack during the crop cycle. Plant BioticPb Biotic attack annotation Exposure to biotic attack; damage du to biotic attack Seed impurity content – Computation Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively NIRS assay. Pct total fatty acids Stearic seed content NIRS - Measurement Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem canker base stem score Stem base Phoma stem cancer severity LfAg Leaf Silver content Leaf The concentration of Silver in leaves Silver content LLS severity - Estimation 10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated. 0-3 disease stage scale Fatty acids functional group content The concentration of components with fatty acids as functional groups from pod and stem material Pod and stem Pod and stem Fatty acids functional group content SdFA Avenasterol proportion of Phytosterols SdAvenasterol_Cp Avenasterol Seed Avenasterol content Seed The percentage of Avenasterol in the total of Phytosterols in seeds Seed The concentration of Chromium in seeds Seed Chromium content Chromium content SdCr root/cm Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) 0-9 Lodging Incidence scale Seed weight - Measurement Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Seed Sodium content The concentration of Sodium in seeds Seed Sodium content SdNa Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid” Plot measure validity – Estimation Beak length length beak Pod beak length wax content SdTotalWax Pod and stem wax content The concentration of wax in the pod and seed material Pod and stem BBCH growth stage 69;end flowering stage Development stage End of flowering time (BBCH 69) Plant End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale) Impurities content Seed SdImpur Seed impurity content Percentage of impurities in a sample of seeds determined by weighing. Seed Stearic acid content SdStearic Seed stearic acid content The concentration of stearic acid in seeds. Linoleic seed content NIRS - Measurement NIRS assay. Pct total fatty acids LLS resistance - Estimation 10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7. Absence or presence of powdery mildew Powdery mildew presence Plant Presence (1) or absence (0) of powdery mildew LfCa Leaf Calcium content The concentration of Calcium in leaves Calcium content Leaf Verticilium wilt presence Plant Absence or presence of Verticilium wilt on the plant Verticilium wilt presence mg Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Stem length - Measurement Powdery mildew presence - Estimation Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1 regularity stand - Estimation Visual notations (scale 0 - 9) Potassium content LfK Leaf Potassium content The concentration of Potassium in leaves Leaf plant/m2 Phoma stem canker upper stem lesion score Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity Upper stem Scoring of the regularity of the plant stand Regularity of plant stand Stand Plant Pod and stem SdBranched Pod and stem branched Alcyl-chain content The concentration of all compounds with branched alcyl chains from pod and stem material branched Alcyl-chain content SdAld The concentration of components with aldehyde functional groups from pod and stem material Aldehyde functional group content Pod and stem Aldehyde functional group content Pod and stem 2020-10-19T00:00:02 root Oil yield - Computation Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O) pod Pod number Number of pods on terminal raceme number BBCH growth stage 61;CETIUM growth stage F2 Development stage Plant 10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale) Beginning of flowering time (BBCH 61) plants/10 plants Beginning of pod development (BBCH 71) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Plant lodging incidence The plant lodging incidence. Plant Lodg Lodging incidence Foot length Length of lower stem from ground level to last formed fertile branch foot length Observe damage caused by Weevils (all species), and rate the damage. Weevil damage - Estimation The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded. Sclerotinia incidence (diseased) - Counting plants/plot CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. End of flowering (BBCH 69) date – Estimation plants/6 plants Seed The concentration of Zinc in seeds Seed Zinc content Zinc content SdZn scale_of First flowers (BBCH 60) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. method_of Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Stem Phoma stem cancer severity Phoma stem canker severity