very low damage 2 SdMo_M_mgkg Thomas Alcock, David Salt Nottingham Martin Broadley, Catherine Thomas LRD_Cp_rootcm Nottingham Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC INRA palmiticSeedCont_M_pct Anne Laperche LfV_M_mgkg Nottingham Martin Broadley,Thomas Alcock plntHeight_M_cm Plant Size;Height of plants in an experimental plot;PLANTSIZE INRA Anne Laperche climaticAnnot_E_cmt Anne Laperche Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC INRA SdP_M_mgkg Thomas Alcock, David Salt Nottingham glsSeedCont_M_μmolg Anne Laperche Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS INRA University of Hertfordshire Yongju Huang, John West PhomaLS_Cou_pct 2 Richard Broughton, Frederic Beaudoin SdB-Sitosterol_Cp_pct Rothamsted John Innes Centre Rachel Wells FtLen_M_cm Anne Laperche bioticAnnot_an_text INRA Biotic Stress;Biotic attack during the crop cycle;BIOTIC Yongju Huang, John West University of Hertfordshire PhomaLS_Cou_plnt10plnt SdKeto_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin INRA Plant Lodging;Scoring of the plant lodging of the plot;LODGING lodging_E_0to9 Anne Laperche 6 FertBraNumb_Ct_Fb Rachel Wells John Innes Centre Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC linolenicSeedCont_M_pct INRA Anne Laperche SdSr_M_mgkg Thomas Alcock, David Salt Nottingham Anne Laperche corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO INRA Growth Stage End Flowering;End of flowering on the main inflorescence stageEndFlo_cnt_dayj Thomas Alcock, David Salt Nottingham SdCr_M_mgkg oleicSeedCont_M_pct Anne Laperche Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC INRA Richard Broughton, Frederic Beaudoin Rothamsted SdCampesterol diunsat_Cp_pct high > 90% 1 Rothamsted SdBrassicasterol_M_mgkg Richard Broughton, Frederic Beaudoin Martin Broadley,Thomas Alcock LfSr_M_mgkg Nottingham PRL_M_cm Martin Broadley, Catherine Thomas Nottingham 6 Evidence of stunting SdAlk_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted 60-90% 3 INRA Phoma damage G2 score phomaDmg_CompG2_0to9 Anne Laperche LLSstem_E_pct University of Hertfordshire Yongju Huang, John West Martin Broadley,Thomas Alcock Nottingham LfCr_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdFA_M_mgkg University of Hertfordshire LLSleaf_E_0to7 Yongju Huang, John West INRA hailDmg_E_0to9 Anne Laperche Hail Damage;HAILDMG PodLen_M_cm John Innes Centre Rachel Wells low to intermediate damage 4 Thomas Alcock, David Salt Nottingham SdLi_M_mgkg Thomas Alcock, David Salt Nottingham SdNa_M_mgkg 7 regular 100% girdling (stem dead/lodged)) 7 Nottingham SD_Est_mm Martin Broadley, Catherine Thomas Richard Broughton, Frederic Beaudoin Rothamsted SdStigmasterol_Cp_pct Nottingham LfS_M_mgkg Martin Broadley,Thomas Alcock Yongju Huang, John West PhomaSK_E_0to7 University of Hertfordshire INRA Anne Laperche Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB seedMoist_mlab_pct Martin Broadley,Thomas Alcock LfU_M_mgkg Nottingham Nottingham LfFe_M_mgkg Martin Broadley,Thomas Alcock 3 Thomas Alcock, David Salt Nottingham SdK_M_mgkg Thomas Alcock, David Salt Nottingham SdCu_M_mgkg Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC Anne Laperche stearicSeedCont_M_pct INRA Martin Broadley,Thomas Alcock LfMo_M_mgkg Nottingham Thomas Alcock, David Salt Nottingham SdB_M_mgkg SdRb_M_mgkg Thomas Alcock, David Salt Nottingham MLRL_Cp_cm Nottingham Martin Broadley, Catherine Thomas Anne Laperche INRA Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI NHI_Comp_pct 9 < 10% LfCu_M_mgkg Martin Broadley,Thomas Alcock Nottingham SdAlcI_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdPhytosterol_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin Nottingham LfMg_M_mgkg Martin Broadley,Thomas Alcock SdCycloartenol_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin VertWilt_Cou_pct Yongju Huang, John West University of Hertfordshire SdMn_M_mgkg Thomas Alcock, David Salt Nottingham John Innes Centre Rachel Wells SdWght_M_mg Nottingham LfCd_M_mgkg Martin Broadley,Thomas Alcock INRA seedImpur_M_pct Anne Laperche Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR TRL_Cp_cm Nottingham Martin Broadley, Catherine Thomas regStand_E_0to9 Anne Laperche Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND INRA ≤ 5% girdling 1 oilYield_Comp_qha Oil Yield;Oil yield per ha;OY INRA Anne Laperche 4 Martin Broadley,Thomas Alcock Nottingham LfRb_M_mgkg INRA Crop problems;Crop problems during the cycle.;CROP cropAnnot_E_cmt Anne Laperche 8 Nottingham Martin Broadley,Thomas Alcock LfBa_M_mgkg Martin Broadley,Thomas Alcock LfNa_M_mgkg Nottingham Yongju Huang, John West LLSpods_E_pct University of Hertfordshire Richard Broughton, Frederic Beaudoin Rothamsted SdAvenasterol_M_mgkg University of York Swen Langer, Lenka Havlickova Sda-toc_M_mgkg Nottingham LfSe_M_mgkg Martin Broadley,Thomas Alcock LfPb_M_mgkg Nottingham Martin Broadley,Thomas Alcock SdAs_M_mgkg Thomas Alcock, David Salt Nottingham Pod shattering;Scoring of pods open just before harvest;PODSHAT podShat_E_score INRA Anne Laperche attribute stageG4_cnt_dayj Anne Laperche flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4 INRA Growth Stage G4;30% of the pods have reached their final size SdPb_M_mgkg Thomas Alcock, David Salt Nottingham Richard Broughton, Frederic Beaudoin SdBrassicasterol_Cp_pct Rothamsted entity Yongju Huang, John West University of Hertfordshire PhomaSKupper_E_0to7 acronym 3 low damage University of Hertfordshire PhomaLS_E_0to3 Yongju Huang, John West SdZn_M_mgkg Thomas Alcock, David Salt Nottingham 1 high irregular PowMildew_E_0or1 University of Hertfordshire Yongju Huang, John West Anne Laperche Plot Surface;Surface of the elementary experimental plot;PLOTSURF plotSize_M_m2 INRA no symptoms observable 0 University of York Swen Langer, Lenka Havlickova Sdy-toc_M_mgkg Richard Broughton, Frederic Beaudoin SdAld_M_mgkg Rothamsted John Innes Centre PedLen_M_cm Rachel Wells LfTi_M_mgkg Nottingham Martin Broadley,Thomas Alcock 0 NA SdMg_M_mgkg Thomas Alcock, David Salt Nottingham Thomas Alcock, David Salt Nottingham SdS_M_mgkg no visible affection 0 INRA stageC1_cnt_dayj Anne Laperche corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1 Growth Stage C1;Bolting date when the first inter-node appears Thomas Alcock, David Salt SdCd_M_mgkg Nottingham severe damage 9 seedMoist_mnirs_pct INRA Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS Anne Laperche plntStnd_M_plntm2 Plant Density;Number of viable plants counted per surface unit;PLANTDENS INRA Anne Laperche Richard Broughton, Frederic Beaudoin Rothamsted SdAlcII_M_mgkg Anne Laperche plotHarvest_M_kg Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST INRA Martin Broadley,Thomas Alcock LfP_M_mgkg Nottingham white blight Sclerot_Cou_plntPlot Yongju Huang, John West University of Hertfordshire seedMoistHarv_mhumi_pct Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV INRA Anne Laperche SdB-Sitosterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdFe_M_mgkg Thomas Alcock, David Salt Nottingham stageG1_cnt_dayj Growth Stage G1;10% of the pods have reached their final size INRA Anne Laperche flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1 Anne Laperche protYield_Comp_qha Prot Yield;Protein yield per ha;PrY INRA Weevils (all species) damage score;charancon Anne Laperche INRA weevilsDmg_E_0to9 INRA linoleicSeedCont_M_pct Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC Anne Laperche Richard Broughton, Frederic Beaudoin Rothamsted SdCampesterol_M_mgkg Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV INRA stagePodDev_cnt_dayj Anne Laperche Rothamsted SdStigmasterol_M_mgkg Richard Broughton, Frederic Beaudoin Richard Broughton, Frederic Beaudoin Rothamsted SdAvenasterol_Cp_pct Rothamsted Richard Broughton, Frederic Beaudoin SdCampesterolDiunsat_M_mgkg >10 spots per plant 3 Martin Broadley,Thomas Alcock LfNi_M_mgkg Nottingham Rachel Wells John Innes Centre PodNumbTermRac_Ct_PodTermRac Martin Broadley, Catherine Thomas LRN_Ct_root Nottingham John Innes Centre Rachel Wells PltHght_M_cm 9 perfectly regular University of Hertfordshire white blight Yongju Huang, John West Sclerot_Cou_plnt6plnt SdCycloartenol_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted Yongju Huang, John West VertWilt_E_0or1 University of Hertfordshire INRA stageF1_cnt_dayj Anne Laperche corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1 Growth Stage F1;First flowers are open Martin Broadley,Thomas Alcock Nottingham LfK_M_mgkg Anne Laperche Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO stageFlo_cnt_dayj INRA Brassica napus ontology Brassica ontology StemWid_M_cm Rachel Wells John Innes Centre 0 No leaf spotting high to severe damage 8 Nottingham Martin Broadley,Thomas Alcock LfAg_M_mgkg Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC erucicSeedCont_M_pct INRA Anne Laperche SdSe_M_mgkg Thomas Alcock, David Salt Nottingham INRA Anne Laperche Protein %;Content of proteins in seeds determined by NIRS;Pr protSeedCont_M_pct Trace of disease 1 protMealCont_M_pct Anne Laperche Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL INRA NA 0 SdBranched_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted INRA Anne Laperche Oil %;Content of oil in seeds determined by NIRS;O oilSeedCont_M_pct Yongju Huang, John West LLSleaf_E_pct University of Hertfordshire 5-10 spots per plant 2 100% girdling ( stem weak/firm) 6 plntStdEmerg_M_plt INRA Anne Laperche Plant Emergence;Percentage of seeds that emerge;EMERG 3 Plants with scattered lesions Martin Broadley,Thomas Alcock LfB_M_mgkg Nottingham Yongju Huang, John West PhomaSKbase_E_0to7 University of Hertfordshire Yongju Huang, John West University of Hertfordshire LeafMiner_Cou_pct SNi_M_mgkg Thomas Alcock, David Salt Nottingham INRA stageF2_cnt_dayj Growth Stage F2;10% of the flowers are open on the main inflorescence elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2 Anne Laperche LfCs_M_mgkg Martin Broadley,Thomas Alcock Nottingham 26-50% girdling 3 Martin Broadley,Thomas Alcock Nottingham LfAs_M_mgkg 1 No lodging LfAl_M_mgkg Nottingham Martin Broadley,Thomas Alcock Growth Stage G5;10% of the seeds have reached maturity seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5 Anne Laperche stageG5_cnt_dayj INRA 2 2-25% girdling Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY INRA stagePodMaturity_cnt_dayj Anne Laperche No disease observable 0 Annotations on measure validity;VALIDATION Anne Laperche INRA validateannot_E_cmt Thomas Alcock, David Salt Nottingham SdCo_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdTotalWax_M_mgkg Nottingham Martin Broadley,Thomas Alcock LfMn_M_mgkg 5 75-100% girdling SdNum_Ct_sd John Innes Centre Rachel Wells Swen Langer, Lenka Havlickova Sdd-toc_M_mgkg University of York NA 0 intermediate damage 5 4 Diseased leaved with a few large lesions of many small leasions, area quite heavily infected 3 irregular Thomas Alcock, David Salt Nottingham SdCa_M_mgkg Culture leaning, without impact 2 high damage 7 LRL_M_cm Martin Broadley, Catherine Thomas Nottingham Richard Broughton, Frederic Beaudoin SdCampesterol_Cp_pct Rothamsted Martin Broadley,Thomas Alcock Nottingham LfCa_M_mgkg John Innes Centre Rachel Wells BeakLen_M_cm trace 1 4 51-75% girdling Half green/half diseased, leaf curling and distortion 5 Nottingham LfZn_M_mgkg Martin Broadley,Thomas Alcock 5 moderately regular 5 30-60% PodWid_M_cm John Innes Centre Rachel Wells 6 intermediate to high damage 7 10-30% 4 Plants heavily stunted or dead. 7 5 Average loding <5 spots per plant 1 2 Diseased leaves with non or a few small lesions Light leaf spot severity LLS severity on stem (percentage of stem area covered by LLS) Stem Light leaf spot severity on stems, judged by the percentage of area covered by LLS. Phoma stem canker base stem score Phoma stem cancer severity Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Stem base Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter. Seed sieving - Estimation Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Fertile Branches - Counting 0-7 percentage scale Development stage 10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale) Beginning of pod maturity time (BBCH 81) Plant BBCH growth stage 81;CETIUM growth stage G5 Number of seeds in ten typical pods seed number Seed number Meal protein content Protein content MlProt The concentration of proteins in the meal. Meal 3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry Leaf mineral analysis - Measurement Beginning of pod development (BBCH 71) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Seed Nickel content Nickel content Seed The concentration of Nickel in seeds SdNi The percentage of Stigmasterol in the total of Phytosterols in seeds Stigmasterol proportion of Phytosterols Stigmasterol SdStigmasterol_Cp Seed Seed Sigmasterol content Seed mineral analysis - Measurement 3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg Arsenic content The concentration of Arsenic in leaves LfAs Leaf Arsenic content Leaf NIRS assay. Pct of Seed Dry Weight Protein seed content NIRS - Measurement Strontium content SdSr Seed Seed Strontium content The concentration of Strontium in seeds Seed delta- tocopherol content Sdd-toc Delta- tocopherol content in seeds delta-tocopherol content seed seed Linoleic seed content NIRS - Measurement NIRS assay. Pct total fatty acids BBCH growth stage 71;CETIUM growth stage G1 Development stage 10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale) Beginning of pod development time (BBCH 71) Plant mg/kg LfP Leaf Phosphorus content Phosphorus content Leaf The concentration of Phosphorus in leaves Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Plant Plant density PltSd Number of viable plants counted per surface unit Stand Crop stand Plant stand 10 spots per plant]]> Leaf phoma resistance - Estimation The concentration of wax in the pod and seed material Pod and stem Pod and stem wax content wax content SdTotalWax Protein yield PrY Seed protein yield Seed Protein yield per ha The concentration of Lithium in seeds Seed SdLi Seed Lithium content Lithium content Visual observations Crop problems annotations - Estimation Plant damage caused by hail. Hail damage Hail damage HailDmg Plant 0-7 disease stage scale The concentration of Avenasterol in seeds Seed Avenasterol content SdAvenasterol Avenasterol Avenasterol content Seed A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents HPLC (High performance liquid chromatography) analysis - Measurement Copper content SdCu Seed The concentration of Copper in seeds Seed Copper content root The concentration of Vanadium in leaves LfV Leaf Vanadium content Leaf Vanadium content Plant lodging incidence Lodg Lodging incidence Plant The plant lodging incidence. pod number Pod number Number of pods on terminal raceme CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of pod maturity (BBCH 81) date – Estimation Biotic stress annotations - Estimation Visual observations Seed SdGLS Seed glucosinolate content Glucosinolate content The concentration of glucosinolates in seeds. Oil content Seed SdOil Seed oil content The concentration of oil in seeds. Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Stem length - Measurement End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale) End of flowering time (BBCH 69) BBCH growth stage 69;end flowering stage Plant Development stage Sclerotinia incidence (diseased) - Counting Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate). plants/plot Leaf Caesium content Caesium content LfCs The concentration of Caesium in leaves Leaf % pod maturity stage;time to BBCH growth stage 82-99 Plant Pod maturity time (BBCH 82-99) Additional stages for pod maturity according to the BBCH scale (82-99) Development stage The concentration of Manganese in leaves Leaf Manganese content LfMn Leaf Manganese content SdStigmasterol Seed Stigmasterol Stigmasterol content Seed Sigmasterol content The concentration of Stigmasterol in seeds Seed Selenium content SdSe Seed Selenium content The concentration of Selenium in seeds Upper stem Phoma stem canker upper stem lesion score Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity Yield Weight (kg) of raw materials harvested by plot Biomass yield Biomass Linolenic seed content NIRS - Measurement NIRS assay. Pct total fatty acids secondary Alkohol functional group content Pod and stem secondary Alkohol functional group content Pod and stem The concentration of components with secondary alcohol functional groups from pod and stem material SdAlcII Seed Brassicasterol content Brassicasterol proportion of Phytosterols The percentage of Brassicasterol in the total of Phytosterols in seeds Brassicasterol Seed SdBrassicasterol First flowers (BBCH 60) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Sulphur content Seed Sulphur content Seed SdS The concentration of Sulphur in seeds Exposure to biotic attack; damage du to biotic attack BioticPb Biotic attack during the crop cycle. Biotic attack annotation Plant Seed Campesterol The percentage of Campesterol in the total of Phytosterols in seeds SdCampesterol_Cp Campesterol proportion of Phytosterols Seed Campesterol content q/ha Plant stand at emergence – Computation EMERG = (Number of plants obtained / Number of seeds sown) x 100 2020-10-19T00:00:02 Titanium content Leaf Titanium content LfTi Leaf The concentration of Titanium in leaves Molybdenum content The concentration of Molybdenum in seeds Seed Molybdenum content Seed SdMo Seed Cadmium content SdCd Cadmium content Seed The concentration of Cadmium in seeds Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Seed weight - Measurement Copper content The concentration of Copper in leaves Leaf LfCu Leaf Copper content Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image. Stem witdh - Measurement SdNa Seed Sodium content The concentration of Sodium in seeds Seed Sodium content Light leaf spot resistance LLS severity on leaf Leaf Light Leaf spot severity on leafs Stand Plant Scoring of the regularity of the plant stand Regularity of plant stand Sclerotinia incidence (diseased) - Counting The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded. 10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot. Phoma incidence (diseased) - Counting The concentration of linolenic acid in seeds. Seed linolenic acid content StLinolenic Linolenic acid content Seed Seed Seed diunsaturated Campesterol content The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds SdCampesterolDiunsat_Cp Campesterol diunsat proportion of Phytosterols Campesterol diunsat Leaf miner incidence (diseased) - Counting 10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner. Phytosterol content Seed total Phytosterol content SdPhytosterol The total concentration of phytosterols in seeds Seed Seed alpha- tocopherol content Sda-toc alpha-tocopherol content Alpha- tocopherol content in seeds seed Surface of the elementary experimental plot Size Plot Plot size PlotSize 0-3 disease stage scale SdPalmitic Palmitic acid content Seed Seed palmitic acid content The concentration of palmitic acid in seeds. Pod and stem branched Alcyl-chain content Pod and stem branched Alcyl-chain content SdBranched The concentration of all compounds with branched alcyl chains from pod and stem material Alkane-functional group content The concentration of components with alkane functional groups from pod and stem material SdAlk Pod and stem Alkane-functional group content Pod and stem CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Addictional flowering stages (BBCH 62-65) date – Estimation The concentration of Chromium in seeds Seed Chromium content Chromium content Seed SdCr Pod width of pods from terminal raceme pod Pod width width Leaf Chromium content Leaf LfCr Chromium content The concentration of Chromium in leaves Plant WeevilsDmg Weevils damage Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil). Weevils damage Pod and stemprimary Alkohol functional group content SdAlcI The concentration of components with primary alcohol functional groups from pod and stem material primary Alkohol functional group content Pod and stem SdProt Protein content Seed Seed protein content The concentration of proteins in seeds. Leaf Sulphur content The concentration of Sulphur in leaves LfS Sulphur content Leaf Oil yield - Computation Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O) Lateral root length LRL Length of the lateral root length Lateral root number Number of fertile branches Number of fertile branches fertile branch Campesterol content Seed Campesterol content SdCampesterol The concentration of Campesterol in seeds Seed Campesterol 10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7. LLS resistance - Estimation Seed Seed Phosphorus content The concentration of Phosphorus in seeds Phosphorus content dSP Seed Cobalt content Seed SdCo Cobalt content The concentration of Cobalt in seeds Pedicel length pedicel Pedicel length of pods from terminal raceme length Seed Seed diameter SD The diameter of a seed diameter Image analysis - Computation The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001) Maximum plant height from ground to tip of terminal raceme PH Plant height height plant Seed impurity content Percentage of impurities in a sample of seeds determined by weighing. Impurities content SdImpur Seed Nitrogen Harvest Index Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root) N content proportion of plant N content Seed N content to total plant N content ratio Seed NHI ClimPb Plant Climatic incidents annotation Exposure to climatic stress Climatic incidents during the crop cycle. Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded. Verticilium wilt presence - Counting Stem Phoma stem cancer severity Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem canker severity mg Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale) First inter-node time (BBCH 31) Plant BBCH growth stage 31;CETIUM growth stage C1 Development stage Leaf Balium content LfBa Leaf Balium content The concentration of Balium in leaves Phoma leaf spot incidence (diseased) - Estimation 10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded gamma-tocopherol content Sdy-toc Seed gamma- tocopherol content seed Gamma- tocopherol conten in seeds Potassium content Leaf LfK Leaf Potassium content The concentration of Potassium in leaves Evaluation of the Phoma lingam infection with the G2 score. The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation. Phoma damage – G2 score – Computation Seed impurity content – Computation Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively Additional stages for pod development according to the BBCH scale (72-79) Development stage Plant pod development stage;time to BBCH growth stages 72-79 Pod development time (BBCH 72-79) LfNi Leaf Nickel content Nickel content The concentration of Nickel in leaves Leaf LfRb The concentration of Rubidium in leaves Leaf Rubidium content Leaf Rubidium content 0-5 percentage bracket scale Seed SdStearic Seed stearic acid content Stearic acid content The concentration of stearic acid in seeds. Leaf Sodium content The concentration of Sodium in leaves Sodium content Leaf LfNa SdCa Calcium content Seed Calcium content The concentration of Calcium in seeds Seed Oil yield Plant OY Oil yield Oil yield Aluminium content LfAl Leaf Aluminium content Leaf The concentration of Aluminium in leaves 0-9 Regularity scale Seed Rubidium content Rubidium content SdRb Seed The concentration of Rubidium in seeds Avenasterol The percentage of Avenasterol in the total of Phytosterols in seeds Seed SdAvenasterol_Cp Seed Avenasterol content Avenasterol proportion of Phytosterols Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr) Seed protein Yield - Computation Seed B-Sitosterol content Seed B-Sitosterol B-Sitosterol content The concentration of B-Sitosterol in seeds SdB-Sitosterol pod/20cm of terminal raceme Protein meal content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight mm Incidence with leaf miner The percentage of plants that showed presence of leaf miner from a subsample of a plot Leaf miner incidence Leaf The concentration of Lead in seeds SdPb Seed Seed Lead content Lead content Potassium content Seed Seed Potassium content The concentration of Potassium in seeds SdK Sclerotinia incidence No of plants with Sclerotinia (out of six) Plant Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. GLS seed content NIRS - Measurement NIRS assay Quantity per Seed Dry Weight Leaf Leaf phoma resistance Severity assessment of phoma leaf spot Phoma leaf spot severity SdFe Iron content Seed The concentration of Iron in seeds Seed Iron content CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod development stages (BBCH 72-79) date – Estimation Aldehyde functional group content Pod and stem Aldehyde functional group content Pod and stem SdAld The concentration of components with aldehyde functional groups from pod and stem material * 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.). * Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers. * Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C. * Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. * After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS. * GC program: * Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes. * Inlet temp: 280°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement Palmitic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Comment Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Pod Number - Counting Incidence of Phoma leaf spot The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot Leaf Phoma incidence Visual observations Climatic incidents annotions - Estimation Erucid seed content NIRS - Measurement NIRS assay. Pct total fatty acids LLS incidence (diseased) - Estimation 10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%. Exposure to crop problems;damage from crop problem Plant Crop problems during the crop cycle. Crop problems annotation CropPb Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging Plant lodging incidence - Estimation Light leaf spot severity Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS. Pod LLS severity on pods (percentage of pod area covered by LLS) Count the number of plants on linear sections defined in each sowing line at the exception of the border lines Plant stand - Counting Humidimeter (sample of seeds) Seed moisture content humidifier - Measurement SdErucic The concentration of erucic acid in seeds. Seed erucic acid content Erucid acid content Seed Linoleic acid content Seed StLinoleic Seed linoleic acid content The concentration of linoleic acid in seeds. Visual notations (scale 0 - 9) Pod shattering - Estimation boolean SdCycloartenol_Cp Seed Cycloartenol content Seed The percentage of Cycloartenol in the total of Phytosterols in seeds Cycloartenol proportion of Phytosterols Cycloartenol Oil seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester plotharvest - Measurement Oleic acid content Seed StOleic Seed oleic acid content The concentration of oleic acid in seeds. Scoring of presence or absence of verticilium wilt symptoms across whole plant. Verticilium wilt presence - Estimation Pod beak length - Measurement Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant. The concentration of Iron in leaves Leaf Iron content Leaf Iron content LfFe day-of-year RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN). Image analysis - Counting plant/m2 LfMg Leaf Magnesium content Leaf Magnesium content The concentration of Magnesium in leaves The concentration of Cycloartenol in seeds Seed Cycloartenol content SdCycloartenol Seed Cycloartenol Cycloartenol content Withering disease caused by the agent Leptosphaeria maculans (Phoma). Plant Phoma damage Phoma damage Withering disease PhomaDmg 0-9 Damage/Incidence scale Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pedicel length - Measurement cm MLRL The mean length of a lateral root of that root system Mean lateral root length Lateral root average length The concentration of components with fatty acids as functional groups from pod and stem material Pod and stem Fatty acids functional group content SdFA Pod and stem Fatty acids functional group content Leaf Leaf Selenium content The concentration of Selenium in leaves Selenium content LfSe Total root length length Total root Length of all roots TRL End of flowering (BBCH 69) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod maturity stages (BBCH 82-99) date – Estimation Foot length Length of lower stem from ground level to last formed fertile branch length foot SdBrassicasterol Brassicasterol content Brassicasterol Seed The concentration of Brassicasterol in seeds Seed Brassicasterol content Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation Seed moisture NIRS - Measurement NIRS assay Lateral root density The mean density of lateral roots along the primary root Lateral root LRD density The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols Relative seed Phytosterol content - Computation LfB Leaf Boron content Leaf Boron content The concentration of Boron in leaves Seed Moisture content SdMoist Seed moisture content Seed moisture content. Assessment on measure validity. Measure validity Valid Plot Plot measure validity Lead content LfPb Leaf Leaf Lead content The concentration of Lead in leaves plants/10 plants CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of flowering (BBCH 61) date - Estimation The number of plants that showed presence of phoma leaf spots from a subsample of a plot Plant Phoma leaf spots Number of plants with phoma leaf spots LLS severity - Estimation 10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated. Seed Plant stand at emergence PltSdEm Plant emergence Percentage of seeds that emerge. Pod width - Measurement Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Cadmium content LfCd Leaf Cadmium content The concentration of Cadmium in leaves Leaf Nitrate Harvest index - Computation Samples from HI assay are analysed for N_%DW in the different fractions Leaf Molybdenum content Molybdenum content The concentration of Molybdenum in leaves Leaf LfMo Weevil damage - Estimation Observe damage caused by Weevils (all species), and rate the damage. method_of CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First inter-node (BBCH 31) date – Estimation Plant Verticilium wilt presence Absence or presence of Verticilium wilt on the plant Verticilium wilt presence The concentration of Arsenic in seeds Seed Seed Arsenic content Arsenic content SdAs 10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated. LLS severity - Estimation Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively Seed moisture content – Computation Seed Boron content SdB The concentration of Boron in seeds Seed Boron content BBCH growth stage 73;CETIUM growth stage G4 30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale) Early pod development time (BBCH 73) Development stage Plant Leaf The concentration of Uranium in leaves LfU Uranium content Leaf Uranium content regularity stand - Estimation Visual notations (scale 0 - 9) Seed B-Sitosterol B-Sitosterol proportion of Phytosterols SdB-Sitosterol_Cp The percentage of B-Sitosterol in the total of Phytosterols in seeds Seed B-Sitosterol content The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation LfZn Zinc content Leaf Zinc content Leaf The concentration of Zinc in leaves LfAg Leaf Silver content The concentration of Silver in leaves Silver content Leaf kg Powdery mildew presence - Estimation Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1 plants/6 plants Leaf Strontium content LfSr Leaf The concentration of Strontium in leaves Strontium content Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod length - Measurement The concentration of Zinc in seeds Zinc content SdZn Seed Seed Zinc content First flowers time (BBCH 60) Plant First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale) BBCH growth stage 60;CETIUM growth stage F1 Development stage Pod and stem Ketone functional group content The concentration of components with ketone functional groups from pod and stem material SdKeto Pod and stem Ketone functional group content Seed moisture content Seed moisture content. SdMoist Moisture content Seed Seed Magnesium content SdMg The concentration of Magnesium in seeds Seed Magnesium content Seed The concentration of Manganese in seeds SdMn Manganese content Seed Manganese content NIRS assay. Pct total fatty acids Oleic seed content NIRS - Measurement Length of the primary root Primary root lengh length PRL Primary root Campesterol diunsat SdCampesterolDiunsat Seed The concentration of diunsaturated Campesterol in seeds Seed diunsaturated Campesterol content diunsaturated Campesterol content No of plants with Sclerotinia (per plot) Sclerotinia incidence Plant Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. Pod length of pods from terminal raceme length Pod length pod PodShat Shattering Pod Pod shattering Proportion of open pods (assessment) Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Plant height - Measurement Light leaf spot incidence The percentage of plants that showed presence of light leaf spot incidence on leaves LLS incidence on leaf Leaf Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation The concentration of Calcium in leaves Leaf Calcium content Calcium content Leaf LfCa Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid” Plot measure validity – Estimation variable_of Presence (1) or absence (0) of powdery mildew Powdery mildew presence Absence or presence of powdery mildew Plant Plant height - Measurement Height is measured from ground to the top of the plants using a dipstick graduated in cm HailDmg - Estimation Score the damage caused by hail. Early pod development (BBCH 73) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. number Lateral root LRN Number of lateral roots Lateral root number scale_of The percentage of plants that showed presence of leaf miner from a subsample of a plot Plant Verticilium wilt presence Verticillium wilt incidence Development stage Flowering time (BBCH 62-65) Additional flowering stages according to the BBCH scale (62-65) Plant flowering stage;time to BBCH growth stage 62-65;Anthesis * Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation. * Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step. * 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples. * Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour. * Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials. * A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions. * The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes. * 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used). * The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane. * Samples are vortexed, centrifuged and the upper hexane phase is removed. * A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first. * Samples are dried in a rotary evaporator or under a stream of nitrogen. * Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID). *GC program: * Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes. * Inlet temp: 325°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement μmol/g beak length Beak length Pod beak length Seed weight seed Seed weight of ten typical pods weight Plot Size – Computation Surface is determined by the experimental design 0-9 Lodging Incidence scale root/cm Stearic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Plant 10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale) BBCH growth stage 61;CETIUM growth stage F2 Beginning of flowering time (BBCH 61) Development stage fertile branch Stem width stem width Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Seed Number - Counting