3 2 2-25% girdling Richard Broughton, Frederic Beaudoin Rothamsted SdAvenasterol_M_mgkg LfNi_M_mgkg Martin Broadley,Thomas Alcock Nottingham Nottingham SdK_M_mgkg Thomas Alcock, David Salt Martin Broadley,Thomas Alcock Nottingham LfU_M_mgkg SNi_M_mgkg Nottingham Thomas Alcock, David Salt Thomas Alcock, David Salt SdCd_M_mgkg Nottingham Anne Laperche seedMoistHarv_mhumi_pct Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV INRA INRA seedMoist_mnirs_pct Anne Laperche Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS SdNa_M_mgkg Nottingham Thomas Alcock, David Salt LfMg_M_mgkg Martin Broadley,Thomas Alcock Nottingham SdCampesterol_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin SdStigmasterol_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted University of Hertfordshire VertWilt_E_0or1 Yongju Huang, John West SdAs_M_mgkg Nottingham Thomas Alcock, David Salt Anne Laperche Growth Stage G5;10% of the seeds have reached maturity INRA seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5 stageG5_cnt_dayj INRA NHI_Comp_pct Anne Laperche Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI seedImpur_M_pct Anne Laperche Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR INRA Rachel Wells John Innes Centre SdWght_M_mg Martin Broadley, Catherine Thomas TRL_Cp_cm Nottingham 3 >10 spots per plant SdLi_M_mgkg Nottingham Thomas Alcock, David Salt INRA Anne Laperche plntHeight_M_cm Plant Size;Height of plants in an experimental plot;PLANTSIZE Sdy-toc_M_mgkg University of York Swen Langer, Lenka Havlickova Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO INRA Anne Laperche stageFlo_cnt_dayj Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC oleicSeedCont_M_pct INRA Anne Laperche Rachel Wells FertBraNumb_Ct_Fb John Innes Centre LfAg_M_mgkg Nottingham Martin Broadley,Thomas Alcock PowMildew_E_0or1 Yongju Huang, John West University of Hertfordshire Martin Broadley,Thomas Alcock LfSe_M_mgkg Nottingham University of Hertfordshire Yongju Huang, John West Sclerot_Cou_plnt6plnt white blight Rothamsted SdAld_M_mgkg Richard Broughton, Frederic Beaudoin Nottingham SdRb_M_mgkg Thomas Alcock, David Salt Nottingham LfSr_M_mgkg Martin Broadley,Thomas Alcock Anne Laperche Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC INRA erucicSeedCont_M_pct Nottingham Thomas Alcock, David Salt SdB_M_mgkg Rachel Wells PodLen_M_cm John Innes Centre Yongju Huang, John West University of Hertfordshire LeafMiner_Cou_pct Martin Broadley,Thomas Alcock LfAl_M_mgkg Nottingham Evidence of stunting 6 stagePodMaturity_cnt_dayj Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY INRA Anne Laperche INRA Anne Laperche flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1 stageG1_cnt_dayj Growth Stage G1;10% of the pods have reached their final size intermediate damage 5 0 no symptoms observable Martin Broadley,Thomas Alcock Nottingham LfMo_M_mgkg Growth Stage G4;30% of the pods have reached their final size stageG4_cnt_dayj INRA flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4 Anne Laperche Nottingham Martin Broadley,Thomas Alcock LfCd_M_mgkg INRA protMealCont_M_pct Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL Anne Laperche Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV INRA stagePodDev_cnt_dayj Anne Laperche 1 <5 spots per plant University of Hertfordshire Yongju Huang, John West PhomaLS_Cou_plnt10plnt Growth Stage End Flowering;End of flowering on the main inflorescence INRA stageEndFlo_cnt_dayj Anne Laperche corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO oilYield_Comp_qha Anne Laperche Oil Yield;Oil yield per ha;OY INRA Richard Broughton, Frederic Beaudoin SdAlk_M_mgkg Rothamsted SdFe_M_mgkg Nottingham Thomas Alcock, David Salt Nottingham SdP_M_mgkg Thomas Alcock, David Salt SdSe_M_mgkg Nottingham Thomas Alcock, David Salt Yongju Huang, John West University of Hertfordshire LLSleaf_E_pct Martin Broadley, Catherine Thomas Nottingham LRD_Cp_rootcm Martin Broadley,Thomas Alcock LfCu_M_mgkg Nottingham Martin Broadley,Thomas Alcock Nottingham LfRb_M_mgkg Martin Broadley,Thomas Alcock Nottingham LfFe_M_mgkg protYield_Comp_qha INRA Prot Yield;Protein yield per ha;PrY Anne Laperche Rothamsted SdStigmasterol_M_mgkg Richard Broughton, Frederic Beaudoin Anne Laperche Crop problems;Crop problems during the cycle.;CROP cropAnnot_E_cmt INRA 7 high damage attribute SdCo_M_mgkg Thomas Alcock, David Salt Nottingham Plant Emergence;Percentage of seeds that emerge;EMERG INRA plntStdEmerg_M_plt Anne Laperche 2 5-10 spots per plant 100% girdling ( stem weak/firm) 6 Martin Broadley,Thomas Alcock Nottingham LfCs_M_mgkg SdMn_M_mgkg Nottingham Thomas Alcock, David Salt 1 high irregular Protein %;Content of proteins in seeds determined by NIRS;Pr Anne Laperche INRA protSeedCont_M_pct Rothamsted Richard Broughton, Frederic Beaudoin SdTotalWax_M_mgkg Martin Broadley,Thomas Alcock Nottingham LfK_M_mgkg Yongju Huang, John West University of Hertfordshire PhomaSKbase_E_0to7 Martin Broadley,Thomas Alcock Nottingham LfP_M_mgkg University of Hertfordshire Yongju Huang, John West PhomaSKupper_E_0to7 3 26-50% girdling John Innes Centre PodWid_M_cm Rachel Wells trace 1 low to intermediate damage 4 oilSeedCont_M_pct INRA Oil %;Content of oil in seeds determined by NIRS;O Anne Laperche Rachel Wells FtLen_M_cm John Innes Centre stageF2_cnt_dayj INRA Growth Stage F2;10% of the flowers are open on the main inflorescence elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2 Anne Laperche John Innes Centre PedLen_M_cm Rachel Wells Anne Laperche Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC climaticAnnot_E_cmt INRA Thomas Alcock, David Salt SdPb_M_mgkg Nottingham Sda-toc_M_mgkg Swen Langer, Lenka Havlickova University of York University of Hertfordshire Yongju Huang, John West LLSstem_E_pct 6 Thomas Alcock, David Salt SdS_M_mgkg Nottingham Richard Broughton, Frederic Beaudoin Rothamsted SdFA_M_mgkg Rothamsted SdCampesterolDiunsat_M_mgkg Richard Broughton, Frederic Beaudoin Yongju Huang, John West University of Hertfordshire PhomaLS_Cou_pct Yongju Huang, John West PhomaLS_E_0to3 University of Hertfordshire Rothamsted SdCampesterol_Cp_pct Richard Broughton, Frederic Beaudoin INRA Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC Anne Laperche stearicSeedCont_M_pct StemWid_M_cm Rachel Wells John Innes Centre Martin Broadley, Catherine Thomas LRL_M_cm Nottingham Martin Broadley,Thomas Alcock Nottingham LfB_M_mgkg Nottingham SdMg_M_mgkg Thomas Alcock, David Salt Rothamsted SdAvenasterol_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted Richard Broughton, Frederic Beaudoin SdB-Sitosterol_Cp_pct INRA Anne Laperche Pod shattering;Scoring of pods open just before harvest;PODSHAT podShat_E_score LfNa_M_mgkg Martin Broadley,Thomas Alcock Nottingham 9 < 10% INRA hailDmg_E_0to9 Hail Damage;HAILDMG Anne Laperche Anne Laperche lodging_E_0to9 Plant Lodging;Scoring of the plant lodging of the plot;LODGING INRA SdCa_M_mgkg Nottingham Thomas Alcock, David Salt Sdd-toc_M_mgkg Swen Langer, Lenka Havlickova University of York Thomas Alcock, David Salt SdSr_M_mgkg Nottingham Anne Laperche stageF1_cnt_dayj INRA corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1 Growth Stage F1;First flowers are open Martin Broadley,Thomas Alcock Nottingham LfCr_M_mgkg Martin Broadley,Thomas Alcock Nottingham LfZn_M_mgkg Rothamsted SdB-Sitosterol_M_mgkg Richard Broughton, Frederic Beaudoin Yongju Huang, John West LLSleaf_E_0to7 University of Hertfordshire Martin Broadley,Thomas Alcock Nottingham LfMn_M_mgkg phomaDmg_CompG2_0to9 Phoma damage G2 score Anne Laperche INRA Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND INRA Anne Laperche regStand_E_0to9 SdBrassicasterol_Cp_pct Rothamsted Richard Broughton, Frederic Beaudoin INRA Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC palmiticSeedCont_M_pct Anne Laperche PRL_M_cm Martin Broadley, Catherine Thomas Nottingham John Innes Centre Rachel Wells PodNumbTermRac_Ct_PodTermRac Nottingham LfCa_M_mgkg Martin Broadley,Thomas Alcock Martin Broadley, Catherine Thomas SD_Est_mm Nottingham Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC INRA linolenicSeedCont_M_pct Anne Laperche MLRL_Cp_cm Martin Broadley, Catherine Thomas Nottingham 4 Diseased leaved with a few large lesions of many small leasions, area quite heavily infected No leaf spotting 0 SdBrassicasterol_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Richard Broughton, Frederic Beaudoin SdCycloartenol_Cp_pct Rothamsted entity PltHght_M_cm John Innes Centre Rachel Wells Nottingham LRN_Ct_root Martin Broadley, Catherine Thomas Martin Broadley,Thomas Alcock Nottingham LfV_M_mgkg Martin Broadley,Thomas Alcock Nottingham LfTi_M_mgkg Biotic Stress;Biotic attack during the crop cycle;BIOTIC INRA Anne Laperche bioticAnnot_an_text Growth Stage C1;Bolting date when the first inter-node appears INRA corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1 Anne Laperche stageC1_cnt_dayj Weevils (all species) damage score;charancon INRA Anne Laperche weevilsDmg_E_0to9 SdCycloartenol_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin Thomas Alcock, David Salt SdMo_M_mgkg Nottingham Average loding 5 John Innes Centre SdNum_Ct_sd Rachel Wells 4 Thomas Alcock, David Salt SdCu_M_mgkg Nottingham SdCr_M_mgkg Nottingham Thomas Alcock, David Salt Nottingham LfBa_M_mgkg Martin Broadley,Thomas Alcock University of Hertfordshire Yongju Huang, John West LLSpods_E_pct Plants heavily stunted or dead. 7 Plot Surface;Surface of the elementary experimental plot;PLOTSURF INRA plotSize_M_m2 Anne Laperche LfPb_M_mgkg Martin Broadley,Thomas Alcock Nottingham Yongju Huang, John West white blight University of Hertfordshire Sclerot_Cou_plntPlot Rachel Wells BeakLen_M_cm John Innes Centre Thomas Alcock, David Salt SdZn_M_mgkg Nottingham Annotations on measure validity;VALIDATION INRA validateannot_E_cmt Anne Laperche SdKeto_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Half green/half diseased, leaf curling and distortion 5 Anne Laperche glsSeedCont_M_μmolg Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS INRA Yongju Huang, John West University of Hertfordshire PhomaSK_E_0to7 INRA seedMoist_mlab_pct Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB Anne Laperche 1 No lodging 6 intermediate to high damage 7 regular INRA linoleicSeedCont_M_pct Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC Anne Laperche Brassica ontology Brassica napus ontology Martin Broadley,Thomas Alcock Nottingham LfAs_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted SdBranched_M_mgkg Richard Broughton, Frederic Beaudoin SdAlcII_M_mgkg Rothamsted 0 NA 30-60% 5 Yongju Huang, John West University of Hertfordshire VertWilt_Cou_pct INRA Plant Density;Number of viable plants counted per surface unit;PLANTDENS Anne Laperche plntStnd_M_plntm2 2 very low damage Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST Anne Laperche plotHarvest_M_kg INRA Rothamsted SdPhytosterol_M_mgkg Richard Broughton, Frederic Beaudoin no visible affection 0 4 51-75% girdling Rothamsted SdAlcI_M_mgkg Richard Broughton, Frederic Beaudoin NA 0 4 5 75-100% girdling 8 high to severe damage acronym LfS_M_mgkg Martin Broadley,Thomas Alcock Nottingham 7 100% girdling (stem dead/lodged)) 8 2 Culture leaning, without impact Rothamsted SdCampesterol diunsat_Cp_pct Richard Broughton, Frederic Beaudoin irregular 3 1 Trace of disease 1 ≤ 5% girdling 10-30% 7 high > 90% 1 Plants with scattered lesions 3 perfectly regular 9 severe damage 9 Diseased leaves with non or a few small lesions 2 low damage 3 2 No disease observable 0 3 60-90% moderately regular 5 0 NA Alkane-functional group content Pod and stem Alkane-functional group content The concentration of components with alkane functional groups from pod and stem material SdAlk Pod and stem * 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.). * Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers. * Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C. * Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. * After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS. * GC program: * Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes. * Inlet temp: 280°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement Seed Molybdenum content Molybdenum content Seed SdMo The concentration of Molybdenum in seeds Visual notations (scale 0 - 9) Pod shattering - Estimation Seed sieving - Estimation Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter. SdMg The concentration of Magnesium in seeds Seed Magnesium content Magnesium content Seed Seed oleic acid content Seed StOleic Oleic acid content The concentration of oleic acid in seeds. BBCH growth stage 69;end flowering stage Development stage End of flowering time (BBCH 69) End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale) Plant Pod Shattering Pod shattering PodShat Proportion of open pods (assessment) The percentage of Avenasterol in the total of Phytosterols in seeds Seed Seed Avenasterol content Avenasterol Avenasterol proportion of Phytosterols SdAvenasterol_Cp SdPhytosterol Seed total Phytosterol content Seed Phytosterol content The total concentration of phytosterols in seeds Seed Avenasterol Avenasterol content The concentration of Avenasterol in seeds Seed Avenasterol content SdAvenasterol CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod maturity stages (BBCH 82-99) date – Estimation The concentration of Iron in leaves Leaf Iron content Iron content Leaf LfFe LfV Leaf Vanadium content Vanadium content The concentration of Vanadium in leaves Leaf Leaf Potassium content LfK The concentration of Potassium in leaves Potassium content Leaf Leaf mineral analysis - Measurement 3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry LLS severity on pods (percentage of pod area covered by LLS) Pod Light leaf spot severity Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS. Seed moisture content Seed moisture content. SdMoist Moisture content Seed Pod and stem secondary Alkohol functional group content secondary Alkohol functional group content The concentration of components with secondary alcohol functional groups from pod and stem material Pod and stem SdAlcII Image analysis - Counting RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN). Additional stages for pod development according to the BBCH scale (72-79) Pod development time (BBCH 72-79) Plant Development stage pod development stage;time to BBCH growth stages 72-79 Leaf LfSe Leaf Selenium content Selenium content The concentration of Selenium in leaves mg/kg Magnesium content LfMg Leaf Magnesium content The concentration of Magnesium in leaves Leaf Evaluation of the Phoma lingam infection with the G2 score. The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation. Phoma damage – G2 score – Computation Plant Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil). WeevilsDmg Weevils damage Weevils damage Calcium content Leaf LfCa The concentration of Calcium in leaves Leaf Calcium content Climatic incidents annotation ClimPb Exposure to climatic stress Plant Climatic incidents during the crop cycle. plants/6 plants StLinoleic The concentration of linoleic acid in seeds. Seed linoleic acid content Seed Linoleic acid content PH Plant height Maximum plant height from ground to tip of terminal raceme plant height 3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg Seed mineral analysis - Measurement Seed Sigmasterol content Stigmasterol Seed SdStigmasterol Stigmasterol content The concentration of Stigmasterol in seeds Leaf Zinc content The concentration of Zinc in leaves LfZn Leaf Zinc content 2020-10-19T00:00:02 The concentration of Titanium in leaves LfTi Leaf Titanium content Titanium content Leaf First flowers (BBCH 60) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Visual notations (scale 0 - 9) regularity stand - Estimation Seed Seed stearic acid content SdStearic The concentration of stearic acid in seeds. Stearic acid content Campesterol proportion of Phytosterols SdCampesterol_Cp Seed Campesterol content The percentage of Campesterol in the total of Phytosterols in seeds Campesterol Seed Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Fertile Branches - Counting Seed Strontium content SdSr Seed The concentration of Strontium in seeds Strontium content Early pod development (BBCH 73) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. 10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale) BBCH growth stage 61;CETIUM growth stage F2 Plant Beginning of flowering time (BBCH 61) Development stage seed number Number of seeds in ten typical pods Seed number Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity Phoma stem canker base stem score Stem base Plant Number of plants with phoma leaf spots The number of plants that showed presence of phoma leaf spots from a subsample of a plot Phoma leaf spots The concentration of Zinc in seeds Seed Zinc content SdZn Seed Zinc content LLS incidence (diseased) - Estimation 10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%. % NIRS assay. Pct total fatty acids Oleic seed content NIRS - Measurement Beginning of pod development (BBCH 71) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Cadmium content Seed SdCd The concentration of Cadmium in seeds Seed Cadmium content The concentration of diunsaturated Campesterol in seeds Campesterol diunsat Seed SdCampesterolDiunsat diunsaturated Campesterol content Seed diunsaturated Campesterol content LRD Lateral root density density Lateral root The mean density of lateral roots along the primary root Nitrate Harvest index - Computation Samples from HI assay are analysed for N_%DW in the different fractions The concentration of Rubidium in leaves Leaf LfRb Leaf Rubidium content Rubidium content Stem length - Measurement Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Seed Sodium content SdNa The concentration of Sodium in seeds Seed Sodium content pod Pod length Pod length of pods from terminal raceme length 10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale) Development stage BBCH growth stage 81;CETIUM growth stage G5 Plant Beginning of pod maturity time (BBCH 81) Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image. Stem witdh - Measurement seed delta-tocopherol content Sdd-toc Seed delta- tocopherol content Delta- tocopherol content in seeds LLS severity on stem (percentage of stem area covered by LLS) Stem Light leaf spot severity Light leaf spot severity on stems, judged by the percentage of area covered by LLS. Seed moisture content humidifier - Measurement Humidimeter (sample of seeds) Plant height - Measurement Height is measured from ground to the top of the plants using a dipstick graduated in cm Cobalt content Seed SdCo Seed Cobalt content The concentration of Cobalt in seeds The concentration of oil in seeds. Seed oil content Seed SdOil Oil content Brassicasterol Brassicasterol proportion of Phytosterols Seed Brassicasterol content The percentage of Brassicasterol in the total of Phytosterols in seeds SdBrassicasterol Seed Exposure to biotic attack; damage du to biotic attack Plant Biotic attack annotation Biotic attack during the crop cycle. BioticPb PlotSize Size Plot size Surface of the elementary experimental plot Plot Linolenic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Seed Phosphorus content Seed dSP The concentration of Phosphorus in seeds Phosphorus content Plot measure validity Plot Valid Assessment on measure validity. Measure validity Surface is determined by the experimental design Plot Size – Computation LfMo Leaf The concentration of Molybdenum in leaves Leaf Molybdenum content Molybdenum content cm The concentration of Balium in leaves Leaf LfBa Balium content Leaf Balium content Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale) First inter-node time (BBCH 31) Development stage Plant BBCH growth stage 31;CETIUM growth stage C1 SdK Potassium content The concentration of Potassium in seeds Seed Seed Potassium content foot length Foot length Length of lower stem from ground level to last formed fertile branch NIRS assay. Pct total fatty acids Erucid seed content NIRS - Measurement Verticilium wilt presence - Estimation Scoring of presence or absence of verticilium wilt symptoms across whole plant. Seed Copper content Seed SdCu Copper content The concentration of Copper in seeds Verticilium wilt presence - Counting Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded. Leaf Aluminium content Leaf The concentration of Aluminium in leaves Aluminium content LfAl pod/20cm of terminal raceme Seed protein Yield - Computation Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr) The mean length of a lateral root of that root system Mean lateral root length Lateral root MLRL average length Plant stand at emergence Plant emergence Percentage of seeds that emerge. PltSdEm Seed Plant density PltSd Number of viable plants counted per surface unit Plant stand Plant Stand Crop stand SdCycloartenol The concentration of Cycloartenol in seeds Seed Cycloartenol content Seed Cycloartenol content Cycloartenol 0-7 percentage scale Plant stand at emergence – Computation EMERG = (Number of plants obtained / Number of seeds sown) x 100 Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem canker upper stem lesion score Phoma stem cancer severity Upper stem Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Seed weight - Measurement diameter The diameter of a seed SD Seed diameter Seed Additional flowering stages according to the BBCH scale (62-65) flowering stage;time to BBCH growth stage 62-65;Anthesis Development stage Flowering time (BBCH 62-65) Plant The concentration of Chromium in leaves Chromium content Leaf Chromium content Leaf LfCr Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod width - Measurement Silver content The concentration of Silver in leaves Leaf Silver content Leaf LfAg Palmitic seed content NIRS - Measurement NIRS assay. Pct total fatty acids Plant lodging incidence - Estimation Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging Seed Seed erucic acid content Erucid acid content The concentration of erucic acid in seeds. SdErucic mm NIRS assay. Pct of Seed Dry Weight Protein meal content NIRS - Measurement The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Biomass yield Weight (kg) of raw materials harvested by plot Yield Biomass Sclerotinia incidence (diseased) - Counting Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate). GLS seed content NIRS - Measurement NIRS assay Quantity per Seed Dry Weight Leaf Copper content Copper content Leaf LfCu The concentration of Copper in leaves Leaf Leaf Manganese content LfMn Manganese content The concentration of Manganese in leaves Leaf LfNa The concentration of Sodium in leaves Sodium content Leaf Sodium content seed Chromium content Seed Seed Chromium content SdCr The concentration of Chromium in seeds Cycloartenol Seed Cycloartenol content Seed SdCycloartenol_Cp The percentage of Cycloartenol in the total of Phytosterols in seeds Cycloartenol proportion of Phytosterols variable_of Seed The concentration of Brassicasterol in seeds Seed Brassicasterol content Brassicasterol content SdBrassicasterol Brassicasterol Leaf Severity assessment of phoma leaf spot Phoma leaf spot severity Leaf phoma resistance Beginning of pod maturity (BBCH 81) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. * Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation. * Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step. * 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples. * Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour. * Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials. * A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions. * The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes. * 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used). * The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane. * Samples are vortexed, centrifuged and the upper hexane phase is removed. * A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first. * Samples are dried in a rotary evaporator or under a stream of nitrogen. * Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID). *GC program: * Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes. * Inlet temp: 325°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement Campesterol Seed Campesterol content SdCampesterol Campesterol content Seed The concentration of Campesterol in seeds plants/10 plants Seed impurity content Impurities content SdImpur Seed Percentage of impurities in a sample of seeds determined by weighing. The percentage of plants that showed presence of leaf miner from a subsample of a plot Plant Verticilium wilt presence Verticillium wilt incidence 10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated. LLS severity - Estimation Score the damage caused by hail. HailDmg - Estimation root/cm 10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated. LLS severity - Estimation First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale) BBCH growth stage 60;CETIUM growth stage F1 Plant First flowers time (BBCH 60) Development stage Pod and stem The concentration of components with primary alcohol functional groups from pod and stem material SdAlcI Pod and stemprimary Alkohol functional group content primary Alkohol functional group content Pod and stem wax content wax content SdTotalWax Pod and stem The concentration of wax in the pod and seed material Pod Number - Counting Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Seed Number - Counting Relative seed Phytosterol content - Computation The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols Meal protein content Meal The concentration of proteins in the meal. Protein content MlProt plant/m2 CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of flowering (BBCH 61) date - Estimation Seed Lead content SdPb Seed The concentration of Lead in seeds Lead content The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Leaf Nickel content The concentration of Nickel in leaves LfNi Leaf Nickel content fertile branch Number of fertile branches Number of fertile branches number q/ha Light Leaf spot severity on leafs Leaf Light leaf spot resistance LLS severity on leaf SdFA Pod and stem The concentration of components with fatty acids as functional groups from pod and stem material Pod and stem Fatty acids functional group content Fatty acids functional group content NIRS assay. Pct total fatty acids Stearic seed content NIRS - Measurement Seed protein content Seed Protein content SdProt The concentration of proteins in seeds. 10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot. Phoma incidence (diseased) - Counting Oil yield Plant OY Oil yield Oil yield Seed impurity content – Computation Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively Plant height - Measurement Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. 0-9 Damage/Incidence scale PRL Primary root lengh length Primary root Length of the primary root Scoring of the regularity of the plant stand Plant Stand Regularity of plant stand Oil yield - Computation Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O) Phoma leaf spot incidence (diseased) - Estimation 10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded Early pod development time (BBCH 73) BBCH growth stage 73;CETIUM growth stage G4 Development stage Plant 30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale) Comment Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant. Pod beak length - Measurement Visual observations Biotic stress annotations - Estimation A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester plotharvest - Measurement SdSe Selenium content The concentration of Selenium in seeds Seed Seed Selenium content LfCs Leaf Caesium content Leaf The concentration of Caesium in leaves Caesium content Leaf LLS incidence on leaf The percentage of plants that showed presence of light leaf spot incidence on leaves Light leaf spot incidence day-of-year Pedicel length of pods from terminal raceme length pedicel Pedicel length seed weight Seed weight of ten typical pods Seed weight plants/plot Seed SdGLS The concentration of glucosinolates in seeds. Seed glucosinolate content Glucosinolate content 0-9 Regularity scale alpha-tocopherol content seed Alpha- tocopherol content in seeds Seed alpha- tocopherol content Sda-toc μmol/g Seed B-Sitosterol content Seed B-Sitosterol proportion of Phytosterols SdB-Sitosterol_Cp B-Sitosterol The percentage of B-Sitosterol in the total of Phytosterols in seeds boolean Pod maturity time (BBCH 82-99) Additional stages for pod maturity according to the BBCH scale (82-99) Development stage Plant pod maturity stage;time to BBCH growth stage 82-99 B-Sitosterol content B-Sitosterol Seed Seed B-Sitosterol content SdB-Sitosterol The concentration of B-Sitosterol in seeds 10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7. LLS resistance - Estimation Seed moisture content – Computation Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively The concentration of Phosphorus in leaves LfP Leaf Phosphorus content Leaf Phosphorus content SdLi The concentration of Lithium in seeds Lithium content Seed Seed Lithium content Length of the lateral root Lateral root LRL Lateral root length length gamma-tocopherol content Gamma- tocopherol conten in seeds Seed gamma- tocopherol content seed Sdy-toc The concentration of Sulphur in leaves LfS Leaf Sulphur content Leaf Sulphur content Leaf miner incidence (diseased) - Counting 10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner. The concentration of Uranium in leaves LfU Uranium content Leaf Leaf Uranium content PhomaDmg Phoma damage Withering disease caused by the agent Leptosphaeria maculans (Phoma). Plant Withering disease Phoma damage Lead content LfPb Leaf Lead content The concentration of Lead in leaves Leaf SdKeto Pod and stem The concentration of components with ketone functional groups from pod and stem material Ketone functional group content Pod and stem Ketone functional group content pod width Pod width of pods from terminal raceme Pod width NIRS assay Seed moisture NIRS - Measurement scale_of NIRS assay. Pct total fatty acids Linoleic seed content NIRS - Measurement Seed moisture content Seed moisture content. SdMoist Seed Moisture content Seed Iron content SdFe The concentration of Iron in seeds Iron content Seed Seed The concentration of Arsenic in seeds SdAs Arsenic content Seed Arsenic content LRN Lateral root Lateral root number number Number of lateral roots Seed N content proportion of plant N content Nitrogen Harvest Index Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root) NHI Seed N content to total plant N content ratio Pod beak length Beak length beak length Hail damage Plant damage caused by hail. HailDmg Plant Hail damage NIRS assay. Pct of Seed Dry Weight Protein seed content NIRS - Measurement Seed Boron content SdB Seed Boron content The concentration of Boron in seeds Leaf phoma resistance - Estimation 10 spots per plant]]> The concentration of all compounds with branched alcyl chains from pod and stem material Pod and stem branched Alcyl-chain content Pod and stem branched Alcyl-chain content SdBranched LfB The concentration of Boron in leaves Leaf Boron content Boron content Leaf Leaf LfSr Strontium content Leaf Strontium content The concentration of Strontium in leaves Sclerotinia incidence No of plants with Sclerotinia (per plot) Plant Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded. Sclerotinia incidence (diseased) - Counting Rubidium content SdRb The concentration of Rubidium in seeds Seed Seed Rubidium content Leaf Leaf Cadmium content The concentration of Cadmium in leaves Cadmium content LfCd Total root Length of all roots TRL Total root length length fertile branch Calcium content Seed Calcium content Seed The concentration of Calcium in seeds SdCa Pod number pod Number of pods on terminal raceme number Campesterol diunsat Seed The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds Campesterol diunsat proportion of Phytosterols Seed diunsaturated Campesterol content SdCampesterolDiunsat_Cp Incidence of Phoma leaf spot Phoma incidence The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot Leaf Observe damage caused by Weevils (all species), and rate the damage. Weevil damage - Estimation Climatic incidents annotions - Estimation Visual observations Stem Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity Phoma stem canker severity stem Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant width Stem width CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. End of flowering (BBCH 69) date – Estimation Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Leaf Leaf Arsenic content Arsenic content LfAs The concentration of Arsenic in leaves Incidence with leaf miner Leaf miner incidence Leaf The percentage of plants that showed presence of leaf miner from a subsample of a plot The concentration of linolenic acid in seeds. StLinolenic Seed linolenic acid content Linolenic acid content Seed SdAld Pod and stem Pod and stem Aldehyde functional group content The concentration of components with aldehyde functional groups from pod and stem material Aldehyde functional group content SdPalmitic The concentration of palmitic acid in seeds. Seed palmitic acid content Seed Palmitic acid content A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents HPLC (High performance liquid chromatography) analysis - Measurement 0-5 percentage bracket scale The plant lodging incidence. Plant lodging incidence Plant Lodg Lodging incidence Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1 Powdery mildew presence - Estimation Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod length - Measurement Plot measure validity – Estimation Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid” Seed Sigmasterol content Stigmasterol Seed Stigmasterol proportion of Phytosterols SdStigmasterol_Cp The percentage of Stigmasterol in the total of Phytosterols in seeds Oil seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight 0-7 disease stage scale Nickel content Seed SdNi Seed Nickel content The concentration of Nickel in seeds kg Plant BBCH growth stage 71;CETIUM growth stage G1 Development stage 10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale) Beginning of pod development time (BBCH 71) 0-3 disease stage scale Additionnal pod development stages (BBCH 72-79) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Sclerotinia incidence Plant Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. No of plants with Sclerotinia (out of six) The concentration of Manganese in seeds SdMn Seed Manganese content Seed Manganese content Plant CropPb Crop problems annotation Crop problems during the crop cycle. Exposure to crop problems;damage from crop problem Seed SdS Sulphur content The concentration of Sulphur in seeds Seed Sulphur content Crop problems annotations - Estimation Visual observations method_of Verticilium wilt presence Absence or presence of Verticilium wilt on the plant Plant Verticilium wilt presence Absence or presence of powdery mildew Presence (1) or absence (0) of powdery mildew Powdery mildew presence Plant CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Addictional flowering stages (BBCH 62-65) date – Estimation 0-9 Lodging Incidence scale Pedicel length - Measurement Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Plant stand - Counting Count the number of plants on linear sections defined in each sowing line at the exception of the border lines mg root Protein yield Seed Protein yield per ha Seed protein yield PrY First inter-node (BBCH 31) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.