no symptoms observable 0 Yongju Huang, John West VertWilt_E_0or1 University of Hertfordshire Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC Anne Laperche INRA oleicSeedCont_M_pct 75-100% girdling 5 John Innes Centre FtLen_M_cm Rachel Wells intermediate damage 5 0 NA Richard Broughton, Frederic Beaudoin Rothamsted SdB-Sitosterol_M_mgkg INRA Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR seedImpur_M_pct Anne Laperche Anne Laperche protYield_Comp_qha INRA Prot Yield;Protein yield per ha;PrY stagePodDev_cnt_dayj Anne Laperche INRA Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV University of Hertfordshire PhomaLS_Cou_plnt10plnt Yongju Huang, John West INRA plotHarvest_M_kg Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST Anne Laperche Anne Laperche Plant Density;Number of viable plants counted per surface unit;PLANTDENS plntStnd_M_plntm2 INRA Martin Broadley,Thomas Alcock Nottingham LfK_M_mgkg SdSe_M_mgkg Thomas Alcock, David Salt Nottingham Anne Laperche erucicSeedCont_M_pct INRA Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC Rachel Wells FertBraNumb_Ct_Fb John Innes Centre Martin Broadley,Thomas Alcock LfP_M_mgkg Nottingham 5 Half green/half diseased, leaf curling and distortion plntStdEmerg_M_plt INRA Plant Emergence;Percentage of seeds that emerge;EMERG Anne Laperche Rachel Wells John Innes Centre PedLen_M_cm Plant Lodging;Scoring of the plant lodging of the plot;LODGING lodging_E_0to9 Anne Laperche INRA perfectly regular 9 3 low damage Martin Broadley,Thomas Alcock LfMg_M_mgkg Nottingham glsSeedCont_M_μmolg INRA Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS Anne Laperche Martin Broadley,Thomas Alcock Nottingham LfCs_M_mgkg Pod shattering;Scoring of pods open just before harvest;PODSHAT podShat_E_score INRA Anne Laperche Nottingham SdS_M_mgkg Thomas Alcock, David Salt regStand_E_0to9 Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND Anne Laperche INRA Rothamsted Richard Broughton, Frederic Beaudoin SdAvenasterol_Cp_pct Anne Laperche Annotations on measure validity;VALIDATION INRA validateannot_E_cmt high damage 7 Anne Laperche bioticAnnot_an_text Biotic Stress;Biotic attack during the crop cycle;BIOTIC INRA LfAs_M_mgkg Nottingham Martin Broadley,Thomas Alcock 2 trace 1 linolenicSeedCont_M_pct Anne Laperche INRA Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC moderately regular 5 INRA Growth Stage G4;30% of the pods have reached their final size stageG4_cnt_dayj flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4 Anne Laperche SdCycloartenol_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin Nottingham Thomas Alcock, David Salt SdLi_M_mgkg Martin Broadley, Catherine Thomas LRL_M_cm Nottingham Richard Broughton, Frederic Beaudoin SdCampesterol_M_mgkg Rothamsted SdB_M_mgkg Nottingham Thomas Alcock, David Salt high > 90% 1 white blight University of Hertfordshire Yongju Huang, John West Sclerot_Cou_plntPlot Martin Broadley,Thomas Alcock Nottingham LfAg_M_mgkg Anne Laperche NHI_Comp_pct INRA Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI Sclerot_Cou_plnt6plnt University of Hertfordshire white blight Yongju Huang, John West Yongju Huang, John West LLSleaf_E_pct University of Hertfordshire stearicSeedCont_M_pct Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC Anne Laperche INRA University of Hertfordshire Yongju Huang, John West PowMildew_E_0or1 Nottingham Thomas Alcock, David Salt SNi_M_mgkg Richard Broughton, Frederic Beaudoin SdBrassicasterol_Cp_pct Rothamsted SdAlcII_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted PodWid_M_cm Rachel Wells John Innes Centre Growth Stage C1;Bolting date when the first inter-node appears INRA Anne Laperche corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1 stageC1_cnt_dayj Thomas Alcock, David Salt SdMo_M_mgkg Nottingham Nottingham LRN_Ct_root Martin Broadley, Catherine Thomas Yongju Huang, John West University of Hertfordshire LLSpods_E_pct Anne Laperche INRA oilSeedCont_M_pct Oil %;Content of oil in seeds determined by NIRS;O John Innes Centre Rachel Wells BeakLen_M_cm Martin Broadley,Thomas Alcock Nottingham LfSr_M_mgkg LfCu_M_mgkg Nottingham Martin Broadley,Thomas Alcock weevilsDmg_E_0to9 Weevils (all species) damage score;charancon Anne Laperche INRA University of Hertfordshire LLSstem_E_pct Yongju Huang, John West SdCo_M_mgkg Nottingham Thomas Alcock, David Salt Evidence of stunting 6 26-50% girdling 3 Thomas Alcock, David Salt SdCu_M_mgkg Nottingham Richard Broughton, Frederic Beaudoin Rothamsted SdKeto_M_mgkg Phoma damage G2 score phomaDmg_CompG2_0to9 Anne Laperche INRA Nottingham PRL_M_cm Martin Broadley, Catherine Thomas Anne Laperche Growth Stage F1;First flowers are open INRA stageF1_cnt_dayj corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1 Martin Broadley,Thomas Alcock Nottingham LfS_M_mgkg LfNa_M_mgkg Martin Broadley,Thomas Alcock Nottingham SdNa_M_mgkg Thomas Alcock, David Salt Nottingham SdCampesterol diunsat_Cp_pct Richard Broughton, Frederic Beaudoin Rothamsted Rachel Wells PodLen_M_cm John Innes Centre Martin Broadley,Thomas Alcock Nottingham LfMo_M_mgkg Richard Broughton, Frederic Beaudoin SdStigmasterol_M_mgkg Rothamsted SdAlcI_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Richard Broughton, Frederic Beaudoin SdAlk_M_mgkg Rothamsted Martin Broadley,Thomas Alcock Nottingham LfAl_M_mgkg ≤ 5% girdling 1 Rachel Wells PltHght_M_cm John Innes Centre Martin Broadley,Thomas Alcock LfSe_M_mgkg Nottingham Martin Broadley,Thomas Alcock LfCd_M_mgkg Nottingham INRA Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO Anne Laperche stageFlo_cnt_dayj LfBa_M_mgkg Martin Broadley,Thomas Alcock Nottingham Richard Broughton, Frederic Beaudoin SdCycloartenol_Cp_pct Rothamsted Thomas Alcock, David Salt Nottingham SdK_M_mgkg Martin Broadley,Thomas Alcock LfTi_M_mgkg Nottingham 3 plotSize_M_m2 Anne Laperche Plot Surface;Surface of the elementary experimental plot;PLOTSURF INRA 2-25% girdling 2 Anne Laperche protMealCont_M_pct Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL INRA Protein %;Content of proteins in seeds determined by NIRS;Pr Anne Laperche protSeedCont_M_pct INRA PhomaLS_E_0to3 University of Hertfordshire Yongju Huang, John West University of Hertfordshire Yongju Huang, John West LLSleaf_E_0to7 University of York Swen Langer, Lenka Havlickova Sda-toc_M_mgkg 4 SdMg_M_mgkg Nottingham Thomas Alcock, David Salt 9 < 10% SdCampesterolDiunsat_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin entity SdBranched_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted No leaf spotting 0 1 <5 spots per plant PhomaSKbase_E_0to7 Yongju Huang, John West University of Hertfordshire SdAs_M_mgkg Thomas Alcock, David Salt Nottingham high to severe damage 8 StemWid_M_cm John Innes Centre Rachel Wells 4 51-75% girdling INRA Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC climaticAnnot_E_cmt Anne Laperche University of Hertfordshire VertWilt_Cou_pct Yongju Huang, John West Nottingham Martin Broadley, Catherine Thomas SD_Est_mm severe damage 9 Anne Laperche INRA stageG1_cnt_dayj flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1 Growth Stage G1;10% of the pods have reached their final size 8 Plants with scattered lesions 3 >10 spots per plant 3 Rothamsted Richard Broughton, Frederic Beaudoin SdCampesterol_Cp_pct Nottingham LfNi_M_mgkg Martin Broadley,Thomas Alcock cropAnnot_E_cmt Anne Laperche Crop problems;Crop problems during the cycle.;CROP INRA Martin Broadley,Thomas Alcock LfZn_M_mgkg Nottingham Martin Broadley,Thomas Alcock Nottingham LfCr_M_mgkg LfFe_M_mgkg Martin Broadley,Thomas Alcock Nottingham Thomas Alcock, David Salt SdMn_M_mgkg Nottingham University of Hertfordshire Yongju Huang, John West PhomaSK_E_0to7 Rothamsted SdB-Sitosterol_Cp_pct Richard Broughton, Frederic Beaudoin Nottingham Martin Broadley, Catherine Thomas TRL_Cp_cm Martin Broadley,Thomas Alcock Nottingham LfRb_M_mgkg LfPb_M_mgkg Martin Broadley,Thomas Alcock Nottingham Martin Broadley, Catherine Thomas LRD_Cp_rootcm Nottingham University of Hertfordshire PhomaLS_Cou_pct Yongju Huang, John West Martin Broadley,Thomas Alcock Nottingham LfB_M_mgkg Thomas Alcock, David Salt SdCa_M_mgkg Nottingham NA 0 Anne Laperche elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2 stageF2_cnt_dayj INRA Growth Stage F2;10% of the flowers are open on the main inflorescence Martin Broadley,Thomas Alcock Nottingham LfU_M_mgkg Yongju Huang, John West University of Hertfordshire PhomaSKupper_E_0to7 1 Trace of disease Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS seedMoist_mnirs_pct Anne Laperche INRA Anne Laperche plntHeight_M_cm Plant Size;Height of plants in an experimental plot;PLANTSIZE INRA Richard Broughton, Frederic Beaudoin SdAvenasterol_M_mgkg Rothamsted oilYield_Comp_qha Anne Laperche INRA Oil Yield;Oil yield per ha;OY Nottingham SdRb_M_mgkg Thomas Alcock, David Salt Nottingham MLRL_Cp_cm Martin Broadley, Catherine Thomas Nottingham Thomas Alcock, David Salt SdZn_M_mgkg Yongju Huang, John West LeafMiner_Cou_pct University of Hertfordshire palmiticSeedCont_M_pct INRA Anne Laperche Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC SdP_M_mgkg Nottingham Thomas Alcock, David Salt SdFA_M_mgkg Richard Broughton, Frederic Beaudoin Rothamsted Rothamsted SdTotalWax_M_mgkg Richard Broughton, Frederic Beaudoin Richard Broughton, Frederic Beaudoin SdAld_M_mgkg Rothamsted 3 irregular Richard Broughton, Frederic Beaudoin SdStigmasterol_Cp_pct Rothamsted INRA seedMoistHarv_mhumi_pct Anne Laperche Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY INRA stagePodMaturity_cnt_dayj Anne Laperche Nottingham SdSr_M_mgkg Thomas Alcock, David Salt 7 regular SdCr_M_mgkg Nottingham Thomas Alcock, David Salt 60-90% 3 Thomas Alcock, David Salt SdFe_M_mgkg Nottingham Growth Stage G5;10% of the seeds have reached maturity seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5 stageG5_cnt_dayj Anne Laperche INRA Swen Langer, Lenka Havlickova University of York Sdy-toc_M_mgkg Swen Langer, Lenka Havlickova University of York Sdd-toc_M_mgkg hailDmg_E_0to9 Anne Laperche Hail Damage;HAILDMG INRA Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB Anne Laperche INRA seedMoist_mlab_pct Nottingham Thomas Alcock, David Salt SdPb_M_mgkg INRA stageEndFlo_cnt_dayj corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO Growth Stage End Flowering;End of flowering on the main inflorescence Anne Laperche LfCa_M_mgkg Martin Broadley,Thomas Alcock Nottingham 7 Plants heavily stunted or dead. 0 NA 5 30-60% linoleicSeedCont_M_pct Anne Laperche INRA Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC low to intermediate damage 4 Nottingham Martin Broadley,Thomas Alcock LfV_M_mgkg 0 no visible affection SdBrassicasterol_M_mgkg Rothamsted Richard Broughton, Frederic Beaudoin 1 high irregular 1 No lodging SdCd_M_mgkg Nottingham Thomas Alcock, David Salt Rothamsted SdPhytosterol_M_mgkg Richard Broughton, Frederic Beaudoin 4 Martin Broadley,Thomas Alcock LfMn_M_mgkg Nottingham 0 No disease observable SdWght_M_mg Rachel Wells John Innes Centre 6 Rachel Wells John Innes Centre SdNum_Ct_sd Diseased leaves with non or a few small lesions 2 intermediate to high damage 6 Rachel Wells PodNumbTermRac_Ct_PodTermRac John Innes Centre 100% girdling ( stem weak/firm) 6 Brassica napus ontology Brassica ontology Average loding 5 100% girdling (stem dead/lodged)) 7 7 10-30% 4 Diseased leaved with a few large lesions of many small leasions, area quite heavily infected attribute 2 Culture leaning, without impact 5-10 spots per plant 2 very low damage 2 acronym Phoma stem canker severity Stem Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity Count the number of plants on linear sections defined in each sowing line at the exception of the border lines Plant stand - Counting kg Plant First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale) First flowers time (BBCH 60) Development stage BBCH growth stage 60;CETIUM growth stage F1 The concentration of Copper in seeds Seed Copper content Copper content Seed SdCu Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter. Seed sieving - Estimation mg/kg Mean lateral root length average length Lateral root MLRL The mean length of a lateral root of that root system cm 0-9 Lodging Incidence scale Pod Proportion of open pods (assessment) Shattering Pod shattering PodShat The concentration of all compounds with branched alcyl chains from pod and stem material SdBranched branched Alcyl-chain content Pod and stem branched Alcyl-chain content Pod and stem GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement * Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation. * Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step. * 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples. * Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour. * Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials. * A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions. * The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes. * 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used). * The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane. * Samples are vortexed, centrifuged and the upper hexane phase is removed. * A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first. * Samples are dried in a rotary evaporator or under a stream of nitrogen. * Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID). *GC program: * Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes. * Inlet temp: 325°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C B-Sitosterol Seed SdB-Sitosterol_Cp Seed B-Sitosterol content B-Sitosterol proportion of Phytosterols The percentage of B-Sitosterol in the total of Phytosterols in seeds 0-5 percentage bracket scale Brassicasterol Brassicasterol content SdBrassicasterol Seed Brassicasterol content Seed The concentration of Brassicasterol in seeds Leaf Lead content The concentration of Lead in leaves Lead content Leaf LfPb BBCH growth stage 81;CETIUM growth stage G5 Plant Development stage Beginning of pod maturity time (BBCH 81) 10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale) Plant flowering stage;time to BBCH growth stage 62-65;Anthesis Flowering time (BBCH 62-65) Development stage Additional flowering stages according to the BBCH scale (62-65) Oleic acid content The concentration of oleic acid in seeds. Seed oleic acid content StOleic Seed Visual notations (scale 0 - 9) Pod shattering - Estimation 0-9 Damage/Incidence scale Nitrogen Harvest Index Seed NHI Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root) Seed N content to total plant N content ratio N content proportion of plant N content Pod and stem Ketone functional group content Ketone functional group content The concentration of components with ketone functional groups from pod and stem material SdKeto Pod and stem The concentration of palmitic acid in seeds. Palmitic acid content Seed SdPalmitic Seed palmitic acid content Avenasterol Avenasterol proportion of Phytosterols Seed SdAvenasterol_Cp Seed Avenasterol content The percentage of Avenasterol in the total of Phytosterols in seeds Score the damage caused by hail. HailDmg - Estimation pedicel Pedicel length Pedicel length of pods from terminal raceme length Additionnal pod development stages (BBCH 72-79) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. SdNa Seed Seed Sodium content Sodium content The concentration of Sodium in seeds number Number of fertile branches Number of fertile branches fertile branch Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1 Powdery mildew presence - Estimation The concentration of Molybdenum in seeds Seed Molybdenum content SdMo Seed Molybdenum content Seed Iron content Seed SdFe Iron content The concentration of Iron in seeds Alkane-functional group content The concentration of components with alkane functional groups from pod and stem material Pod and stem Pod and stem Alkane-functional group content SdAlk Surface is determined by the experimental design Plot Size – Computation The concentration of glucosinolates in seeds. Seed glucosinolate content SdGLS Seed Glucosinolate content Brassicasterol proportion of Phytosterols The percentage of Brassicasterol in the total of Phytosterols in seeds SdBrassicasterol Brassicasterol Seed Brassicasterol content Seed LLS incidence on leaf Leaf The percentage of plants that showed presence of light leaf spot incidence on leaves Light leaf spot incidence SdCycloartenol_Cp Seed Cycloartenol content Seed The percentage of Cycloartenol in the total of Phytosterols in seeds Cycloartenol proportion of Phytosterols Cycloartenol Pod number Number of pods on terminal raceme pod number Seed Seed moisture content. SdMoist Seed moisture content Moisture content The plant lodging incidence. Plant lodging incidence Plant Lodg Lodging incidence Withering disease Phoma damage Withering disease caused by the agent Leptosphaeria maculans (Phoma). Plant Phoma damage PhomaDmg Leaf phoma resistance - Estimation 10 spots per plant]]> The total concentration of phytosterols in seeds Phytosterol content Seed total Phytosterol content SdPhytosterol Seed Climatic incidents during the crop cycle. Exposure to climatic stress ClimPb Plant Climatic incidents annotation Oil yield - Computation Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O) 3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry Leaf mineral analysis - Measurement plants/6 plants GLS seed content NIRS - Measurement NIRS assay Quantity per Seed Dry Weight number Lateral root Lateral root number Number of lateral roots LRN Seed Cycloartenol content Cycloartenol The concentration of Cycloartenol in seeds SdCycloartenol Seed Cycloartenol content Seed mineral analysis - Measurement 3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg Seed Chromium content Chromium content Seed SdCr The concentration of Chromium in seeds variable_of Relative seed Phytosterol content - Computation The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols Linoleic acid content Seed The concentration of linoleic acid in seeds. StLinoleic Seed linoleic acid content % Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Phoma stem cancer severity Phoma stem canker upper stem lesion score Upper stem The concentration of diunsaturated Campesterol in seeds Seed diunsaturated Campesterol content Seed SdCampesterolDiunsat Campesterol diunsat diunsaturated Campesterol content Campesterol content The concentration of Campesterol in seeds Campesterol SdCampesterol Seed Seed Campesterol content length Primary root lengh PRL Primary root Length of the primary root Plant Oil yield OY Oil yield Oil yield Sdy-toc gamma-tocopherol content Gamma- tocopherol conten in seeds Seed gamma- tocopherol content seed The concentration of Vanadium in leaves Leaf Leaf Vanadium content Vanadium content LfV Cadmium content The concentration of Cadmium in leaves LfCd Leaf Leaf Cadmium content Foot length foot Length of lower stem from ground level to last formed fertile branch length scale_of First inter-node (BBCH 31) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. SdCampesterolDiunsat_Cp Seed Seed diunsaturated Campesterol content The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds Campesterol diunsat Campesterol diunsat proportion of Phytosterols GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement * 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.). * Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers. * Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C. * Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. * After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS. * GC program: * Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes. * Inlet temp: 280°C, split-less injection. * Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min. * FID temp: 325°C pod/20cm of terminal raceme Oil seed content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight Seed Seed moisture content Seed moisture content. SdMoist Moisture content Cadmium content SdCd Seed The concentration of Cadmium in seeds Seed Cadmium content Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) HailDmg Hail damage Hail damage Plant Plant damage caused by hail. Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr) Seed protein Yield - Computation Stearic acid content Seed stearic acid content Seed The concentration of stearic acid in seeds. SdStearic Seed The concentration of Boron in seeds Boron content SdB Seed Boron content Seed SdAs The concentration of Arsenic in seeds Arsenic content Seed Arsenic content wax content Pod and stem wax content SdTotalWax Pod and stem The concentration of wax in the pod and seed material Image analysis - Computation Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001) Stem length - Measurement Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Leaf Boron content Leaf LfB Boron content The concentration of Boron in leaves Seed The concentration of Zinc in seeds Seed Zinc content SdZn Zinc content Leaf LfMg Leaf Magnesium content Magnesium content The concentration of Magnesium in leaves 10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner. Leaf miner incidence (diseased) - Counting SdK Seed Seed Potassium content Potassium content The concentration of Potassium in seeds Length of the lateral root Lateral root Lateral root length length LRL Sclerotinia incidence (diseased) - Counting The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded. Beginning of pod maturity (BBCH 81) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. primary Alkohol functional group content SdAlcI Pod and stemprimary Alkohol functional group content Pod and stem The concentration of components with primary alcohol functional groups from pod and stem material The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001) Image analysis - Computation Crop problems during the crop cycle. CropPb Crop problems annotation Exposure to crop problems;damage from crop problem Plant The concentration of proteins in seeds. Seed Protein content Seed protein content SdProt The mean density of lateral roots along the primary root density LRD Lateral root Lateral root density Pod and stem secondary Alkohol functional group content SdAlcII secondary Alkohol functional group content Pod and stem The concentration of components with secondary alcohol functional groups from pod and stem material WeevilsDmg Weevils damage Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil). Weevils damage Plant Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod length - Measurement plants/10 plants root Protein content Meal MlProt Meal protein content The concentration of proteins in the meal. Pod development time (BBCH 72-79) Additional stages for pod development according to the BBCH scale (72-79) Development stage pod development stage;time to BBCH growth stages 72-79 Plant Seed diameter Seed diameter SD The diameter of a seed 0-7 percentage scale Leaf Titanium content LfTi The concentration of Titanium in leaves Leaf Titanium content CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Additionnal pod maturity stages (BBCH 82-99) date – Estimation PltSdEm Plant emergence Seed Plant stand at emergence Percentage of seeds that emerge. Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Seed Number - Counting Seed The concentration of Nickel in seeds SdNi Seed Nickel content Nickel content Rubidium content Leaf The concentration of Rubidium in leaves Leaf Rubidium content LfRb Light leaf spot severity LLS severity on stem (percentage of stem area covered by LLS) Stem Light leaf spot severity on stems, judged by the percentage of area covered by LLS. Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded. Verticilium wilt presence - Counting Leaf Leaf Aluminium content The concentration of Aluminium in leaves LfAl Aluminium content Leaf Phoma leaf spot severity Leaf phoma resistance Severity assessment of phoma leaf spot Plant Absence or presence of Verticilium wilt on the plant Verticilium wilt presence Verticilium wilt presence seed Sclerotinia incidence Plant Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. No of plants with Sclerotinia (out of six) Plant Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering. No of plants with Sclerotinia (per plot) Sclerotinia incidence SdAvenasterol Avenasterol The concentration of Avenasterol in seeds Seed Avenasterol content Seed Avenasterol content A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK) twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK) dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis. Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents HPLC (High performance liquid chromatography) analysis - Measurement Leaf Leaf Copper content LfCu The concentration of Copper in leaves Copper content Biomass yield Biomass Yield Weight (kg) of raw materials harvested by plot 10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale) BBCH growth stage 61;CETIUM growth stage F2 Beginning of flowering time (BBCH 61) Plant Development stage Image analysis - Computation The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001) Leaf Caesium content Caesium content The concentration of Caesium in leaves LfCs Leaf Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging Plant lodging incidence - Estimation The concentration of Strontium in seeds SdSr Seed Seed Strontium content Strontium content Leaf Zinc content Leaf The concentration of Zinc in leaves LfZn Zinc content Number of seeds in ten typical pods seed Seed number number Impurities content Seed impurity content Seed SdImpur Percentage of impurities in a sample of seeds determined by weighing. Seed The concentration of Sulphur in seeds Sulphur content Seed Sulphur content SdS plant/m2 Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Phoma stem cancer severity - Estimation Leaf Silver content The concentration of Silver in leaves LfAg Leaf Silver content LfBa Balium content Leaf Balium content Leaf The concentration of Balium in leaves Leaf Iron content LfFe The concentration of Iron in leaves Iron content Leaf The concentration of Rubidium in seeds Seed Rubidium content SdRb Seed Rubidium content Plant Early pod development time (BBCH 73) 30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale) BBCH growth stage 73;CETIUM growth stage G4 Development stage 0-7 disease stage scale SdStigmasterol_Cp Stigmasterol Seed Sigmasterol content Seed Stigmasterol proportion of Phytosterols The percentage of Stigmasterol in the total of Phytosterols in seeds beak Beak length Pod beak length length boolean Leaf Uranium content Leaf Uranium content LfU The concentration of Uranium in leaves day-of-year RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN). Image analysis - Counting The concentration of Lithium in seeds SdLi Seed Lithium content Seed Lithium content NIRS assay. Pct total fatty acids Erucid seed content NIRS - Measurement Sclerotinia incidence (diseased) - Counting Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate). Plant Verticillium wilt incidence Verticilium wilt presence The percentage of plants that showed presence of leaf miner from a subsample of a plot Plot measure validity – Estimation Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid” The concentration of components with fatty acids as functional groups from pod and stem material Fatty acids functional group content SdFA Pod and stem Pod and stem Fatty acids functional group content Seed weight of ten typical pods Seed weight seed weight Leaf Leaf Calcium content Calcium content The concentration of Calcium in leaves LfCa 10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded Phoma leaf spot incidence (diseased) - Estimation Total root Length of all roots Total root length length TRL Leaf LfP Phosphorus content The concentration of Phosphorus in leaves Leaf Phosphorus content Addictional flowering stages (BBCH 62-65) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS. Pod Light leaf spot severity LLS severity on pods (percentage of pod area covered by LLS) Fertile Branches - Counting Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Leaf Light leaf spot resistance LLS severity on leaf Light Leaf spot severity on leafs LfK Leaf The concentration of Potassium in leaves Leaf Potassium content Potassium content Phoma incidence (diseased) - Counting 10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot. Leaf Manganese content The concentration of Manganese in leaves Leaf Manganese content LfMn q/ha Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged Pod width - Measurement NIRS assay. Pct of Seed Dry Weight Protein seed content NIRS - Measurement Seed Lead content The concentration of Lead in seeds Seed Lead content SdPb method_of Maximum plant height from ground to tip of terminal raceme Plant height height plant PH Powdery mildew presence Absence or presence of powdery mildew Plant Presence (1) or absence (0) of powdery mildew Plot Assessment on measure validity. Plot measure validity Measure validity Valid Comment Pod beak length - Measurement Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant. Strontium content Leaf Strontium content Leaf The concentration of Strontium in leaves LfSr Chromium content Leaf LfCr The concentration of Chromium in leaves Leaf Chromium content The number of plants that showed presence of phoma leaf spots from a subsample of a plot Plant Phoma leaf spots Number of plants with phoma leaf spots fertile branch Linolenic acid content Seed linolenic acid content The concentration of linolenic acid in seeds. Seed StLinolenic Visual observations Climatic incidents annotions - Estimation 0-3 disease stage scale Early pod development (BBCH 73) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Cobalt content SdCo The concentration of Cobalt in seeds Seed Seed Cobalt content The concentration of Magnesium in seeds SdMg Magnesium content Seed Magnesium content Seed Incidence of Phoma leaf spot The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot Leaf Phoma incidence 10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%. LLS incidence (diseased) - Estimation Protein meal content NIRS - Measurement NIRS assay. Pct of Seed Dry Weight Manganese content Seed Manganese content The concentration of Manganese in seeds Seed SdMn Seed moisture content – Computation Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively Seed Calcium content The concentration of Calcium in seeds Calcium content SdCa Seed Leaf The concentration of Sulphur in leaves Sulphur content Leaf Sulphur content LfS Seed alpha- tocopherol content alpha-tocopherol content Alpha- tocopherol content in seeds seed Sda-toc Observe damage caused by Weevils (all species), and rate the damage. Weevil damage - Estimation Visual observations Crop problems annotations - Estimation pod length Pod length of pods from terminal raceme Pod length root/cm Aldehyde functional group content The concentration of components with aldehyde functional groups from pod and stem material Pod and stem SdAld Pod and stem Aldehyde functional group content Verticilium wilt presence - Estimation Scoring of presence or absence of verticilium wilt symptoms across whole plant. Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa Stem base Phoma stem canker base stem score Phoma stem cancer severity Delta- tocopherol content in seeds Seed delta- tocopherol content Sdd-toc seed delta-tocopherol content Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively Seed impurity content – Computation SdCampesterol_Cp The percentage of Campesterol in the total of Phytosterols in seeds Campesterol Seed Campesterol proportion of Phytosterols Seed Campesterol content Seed Sigmasterol content Stigmasterol content Stigmasterol Seed SdStigmasterol The concentration of Stigmasterol in seeds Height is measured from ground to the top of the plants using a dipstick graduated in cm Plant height - Measurement Leaf Leaf Sodium content LfNa The concentration of Sodium in leaves Sodium content The concentration of Molybdenum in leaves LfMo Leaf Leaf Molybdenum content Molybdenum content SdB-Sitosterol Seed B-Sitosterol content Seed B-Sitosterol content The concentration of B-Sitosterol in seeds B-Sitosterol dSP Phosphorus content The concentration of Phosphorus in seeds Seed Phosphorus content Seed NIRS assay. Pct total fatty acids Oleic seed content NIRS - Measurement Plant stand at emergence – Computation EMERG = (Number of plants obtained / Number of seeds sown) x 100 CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Beginning of flowering (BBCH 61) date - Estimation Incidence with leaf miner The percentage of plants that showed presence of leaf miner from a subsample of a plot Leaf miner incidence Leaf 0-9 Regularity scale Seed weight - Measurement Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser. Selenium content Leaf LfSe The concentration of Selenium in leaves Leaf Selenium content Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Plant height - Measurement Seed erucic acid content Erucid acid content The concentration of erucic acid in seeds. SdErucic Seed Nickel content LfNi Leaf Nickel content The concentration of Nickel in leaves Leaf The concentration of Arsenic in leaves Leaf LfAs Leaf Arsenic content Arsenic content Humidimeter (sample of seeds) Seed moisture content humidifier - Measurement Seed protein yield PrY Seed Protein yield Protein yield per ha 10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated. LLS severity - Estimation Seed SdSe Selenium content The concentration of Selenium in seeds Seed Selenium content BBCH growth stage 69;end flowering stage Development stage End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale) End of flowering time (BBCH 69) Plant Plant Stand Regularity of plant stand Scoring of the regularity of the plant stand plants/plot NIRS assay. Pct total fatty acids Palmitic seed content NIRS - Measurement Plant pod maturity stage;time to BBCH growth stage 82-99 Pod maturity time (BBCH 82-99) Development stage Additional stages for pod maturity according to the BBCH scale (82-99) mg A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester plotharvest - Measurement mm PltSd Stand Plant Plant density Plant stand Crop stand Number of viable plants counted per surface unit Phoma stem cancer severity - Estimation Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged)) Plant Biotic attack annotation Biotic attack during the crop cycle. Exposure to biotic attack; damage du to biotic attack BioticPb LLS resistance - Estimation 10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7. Pod width of pods from terminal raceme width pod Pod width Visual notations (scale 0 - 9) regularity stand - Estimation Oil content SdOil Seed The concentration of oil in seeds. Seed oil content Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing. Pod Number - Counting Pedicel length - Measurement Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged First inter-node time (BBCH 31) Development stage Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale) BBCH growth stage 31;CETIUM growth stage C1 Plant Evaluation of the Phoma lingam infection with the G2 score. The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation. Phoma damage – G2 score – Computation 10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale) Plant Beginning of pod development time (BBCH 71) Development stage BBCH growth stage 71;CETIUM growth stage G1 Surface of the elementary experimental plot PlotSize Plot Plot size Size Beginning of pod development (BBCH 71) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. Samples from HI assay are analysed for N_%DW in the different fractions Nitrate Harvest index - Computation LLS severity - Estimation 10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated. Visual observations Biotic stress annotations - Estimation End of flowering (BBCH 69) date – Estimation CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. 2020-10-19T00:00:02 Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image. Stem witdh - Measurement Stem width stem Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant width Linolenic seed content NIRS - Measurement NIRS assay. Pct total fatty acids CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH. First flowers (BBCH 60) date – Estimation Linoleic seed content NIRS - Measurement NIRS assay. Pct total fatty acids NIRS assay Seed moisture NIRS - Measurement μmol/g NIRS assay. Pct total fatty acids Stearic seed content NIRS - Measurement