SdMo_M_mgkg
Nottingham
Thomas Alcock, David Salt
SdCycloartenol_Cp_pct
Rothamsted
Richard Broughton, Frederic Beaudoin
Sda-toc_M_mgkg
University of York
Swen Langer, Lenka Havlickova
hailDmg_E_0to9
Hail Damage;HAILDMG
INRA
Anne Laperche
stageF1_cnt_dayj
Growth Stage F1;First flowers are open
corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1
INRA
Anne Laperche
LfTi_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
LeafMiner_Cou_pct
University of Hertfordshire
Yongju Huang, John West
SdAs_M_mgkg
Nottingham
Thomas Alcock, David Salt
intermediate to high damage
6
LfCu_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
PhomaLS_E_0to3
University of Hertfordshire
Yongju Huang, John West
palmiticSeedCont_M_pct
Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC
INRA
Anne Laperche
SdRb_M_mgkg
Nottingham
Thomas Alcock, David Salt
weevilsDmg_E_0to9
Weevils (all species) damage score;charancon
INRA
Anne Laperche
LfNi_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
8
SdTotalWax_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdCampesterol_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdMg_M_mgkg
Nottingham
Thomas Alcock, David Salt
cropAnnot_E_cmt
Crop problems;Crop problems during the cycle.;CROP
INRA
Anne Laperche
LRN_Ct_root
Nottingham
Martin Broadley, Catherine Thomas
plotSize_M_m2
Plot Surface;Surface of the elementary experimental plot;PLOTSURF
INRA
Anne Laperche
oilSeedCont_M_pct
Oil %;Content of oil in seeds determined by NIRS;O
INRA
Anne Laperche
plntHeight_M_cm
Plant Size;Height of plants in an experimental plot;PLANTSIZE
INRA
Anne Laperche
SD_Est_mm
Nottingham
Martin Broadley, Catherine Thomas
NHI_Comp_pct
Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI
INRA
Anne Laperche
No lodging
1
SdP_M_mgkg
Nottingham
Thomas Alcock, David Salt
LfV_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
LfMo_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
FertBraNumb_Ct_Fb
John Innes Centre
Rachel Wells
linolenicSeedCont_M_pct
Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC
INRA
Anne Laperche
SdCampesterol_Cp_pct
Rothamsted
Richard Broughton, Frederic Beaudoin
SdStigmasterol_Cp_pct
Rothamsted
Richard Broughton, Frederic Beaudoin
BeakLen_M_cm
John Innes Centre
Rachel Wells
PhomaSK_E_0to7
University of Hertfordshire
Yongju Huang, John West
stageFlo_cnt_dayj
Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO
INRA
Anne Laperche
oleicSeedCont_M_pct
Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC
INRA
Anne Laperche
LfP_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
PodLen_M_cm
John Innes Centre
Rachel Wells
seedMoistHarv_mhumi_pct
Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV
INRA
Anne Laperche
Sdy-toc_M_mgkg
University of York
Swen Langer, Lenka Havlickova
SdCycloartenol_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdS_M_mgkg
Nottingham
Thomas Alcock, David Salt
LfMn_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
LfS_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
SdB-Sitosterol_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
LRD_Cp_rootcm
Nottingham
Martin Broadley, Catherine Thomas
seedMoist_mnirs_pct
Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS
INRA
Anne Laperche
phomaDmg_CompG2_0to9
Phoma damage G2 score
INRA
Anne Laperche
high irregular
1
5-10 spots per plant
2
protYield_Comp_qha
Prot Yield;Protein yield per ha;PrY
INRA
Anne Laperche
LLSleaf_E_pct
University of Hertfordshire
Yongju Huang, John West
LfBa_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
LfAl_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
protSeedCont_M_pct
Protein %;Content of proteins in seeds determined by NIRS;Pr
INRA
Anne Laperche
lodging_E_0to9
Plant Lodging;Scoring of the plant lodging of the plot;LODGING
INRA
Anne Laperche
SdFe_M_mgkg
Nottingham
Thomas Alcock, David Salt
>10 spots per plant
3
SdB-Sitosterol_Cp_pct
Rothamsted
Richard Broughton, Frederic Beaudoin
30-60%
5
PhomaSKbase_E_0to7
University of Hertfordshire
Yongju Huang, John West
Culture leaning, without impact
2
2-25% girdling
2
100% girdling ( stem weak/firm)
6
PltHght_M_cm
John Innes Centre
Rachel Wells
75-100% girdling
5
LfK_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
stageG4_cnt_dayj
Growth Stage G4;30% of the pods have reached their final size
flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4
INRA
Anne Laperche
stageG5_cnt_dayj
Growth Stage G5;10% of the seeds have reached maturity
seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5
INRA
Anne Laperche
plotHarvest_M_kg
Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST
INRA
Anne Laperche
high > 90%
1
< 10%
9
PowMildew_E_0or1
University of Hertfordshire
Yongju Huang, John West
LfRb_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
LfCa_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
LfFe_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
SdNa_M_mgkg
Nottingham
Thomas Alcock, David Salt
SdFA_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdK_M_mgkg
Nottingham
Thomas Alcock, David Salt
LfAg_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
PedLen_M_cm
John Innes Centre
Rachel Wells
SdBranched_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
stagePodMaturity_cnt_dayj
Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY
INRA
Anne Laperche
FtLen_M_cm
John Innes Centre
Rachel Wells
SdPb_M_mgkg
Nottingham
Thomas Alcock, David Salt
SdKeto_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
stageG1_cnt_dayj
Growth Stage G1;10% of the pods have reached their final size
flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1
INRA
Anne Laperche
LfZn_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
oilYield_Comp_qha
Oil Yield;Oil yield per ha;OY
INRA
Anne Laperche
SdAvenasterol_Cp_pct
Rothamsted
Richard Broughton, Frederic Beaudoin
SdBrassicasterol_Cp_pct
Rothamsted
Richard Broughton, Frederic Beaudoin
seedMoist_mlab_pct
Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB
INRA
Anne Laperche
LfNa_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
SdAlk_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdAlcI_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
LfB_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
SdLi_M_mgkg
Nottingham
Thomas Alcock, David Salt
SdCo_M_mgkg
Nottingham
Thomas Alcock, David Salt
stageC1_cnt_dayj
Growth Stage C1;Bolting date when the first inter-node appears
corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1
INRA
Anne Laperche
linoleicSeedCont_M_pct
Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC
INRA
Anne Laperche
PhomaLS_Cou_plnt10plnt
University of Hertfordshire
Yongju Huang, John West
high to severe damage
8
SdMn_M_mgkg
Nottingham
Thomas Alcock, David Salt
LRL_M_cm
Nottingham
Martin Broadley, Catherine Thomas
SdSr_M_mgkg
Nottingham
Thomas Alcock, David Salt
regStand_E_0to9
Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND
INRA
Anne Laperche
2
validateannot_E_cmt
Annotations on measure validity;VALIDATION
INRA
Anne Laperche
stagePodDev_cnt_dayj
Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV
INRA
Anne Laperche
StemWid_M_cm
John Innes Centre
Rachel Wells
PodWid_M_cm
John Innes Centre
Rachel Wells
LfCs_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
PhomaSKupper_E_0to7
University of Hertfordshire
Yongju Huang, John West
glsSeedCont_M_μmolg
Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS
INRA
Anne Laperche
trace
1
plntStdEmerg_M_plt
Plant Emergence;Percentage of seeds that emerge;EMERG
INRA
Anne Laperche
Sclerot_Cou_plntPlot
white blight
University of Hertfordshire
Yongju Huang, John West
51-75% girdling
4
VertWilt_Cou_pct
University of Hertfordshire
Yongju Huang, John West
SdCampesterol diunsat_Cp_pct
Rothamsted
Richard Broughton, Frederic Beaudoin
SdAld_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
stageEndFlo_cnt_dayj
Growth Stage End Flowering;End of flowering on the main inflorescence
corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO
INRA
Anne Laperche
LfCr_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
SdBrassicasterol_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdCa_M_mgkg
Nottingham
Thomas Alcock, David Salt
LLSleaf_E_0to7
University of Hertfordshire
Yongju Huang, John West
SdCu_M_mgkg
Nottingham
Thomas Alcock, David Salt
LfU_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
<5 spots per plant
1
LfMg_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
Plants with scattered lesions
3
no symptoms observable
0
erucicSeedCont_M_pct
Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC
INRA
Anne Laperche
SdStigmasterol_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdZn_M_mgkg
Nottingham
Thomas Alcock, David Salt
SdSe_M_mgkg
Nottingham
Thomas Alcock, David Salt
LLSpods_E_pct
University of Hertfordshire
Yongju Huang, John West
stageF2_cnt_dayj
Growth Stage F2;10% of the flowers are open on the main inflorescence
elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2
INRA
Anne Laperche
LfPb_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
No leaf spotting
0
protMealCont_M_pct
Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL
INRA
Anne Laperche
LLSstem_E_pct
University of Hertfordshire
Yongju Huang, John West
SdNum_Ct_sd
John Innes Centre
Rachel Wells
PRL_M_cm
Nottingham
Martin Broadley, Catherine Thomas
plntStnd_M_plntm2
Plant Density;Number of viable plants counted per surface unit;PLANTDENS
INRA
Anne Laperche
≤ 5% girdling
1
TRL_Cp_cm
Nottingham
Martin Broadley, Catherine Thomas
LfCd_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
podShat_E_score
Pod shattering;Scoring of pods open just before harvest;PODSHAT
INRA
Anne Laperche
climaticAnnot_E_cmt
Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC
INRA
Anne Laperche
PodNumbTermRac_Ct_PodTermRac
John Innes Centre
Rachel Wells
perfectly regular
9
severe damage
9
Diseased leaves with non or a few small lesions
2
SdPhytosterol_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
10-30%
7
100% girdling (stem dead/lodged))
7
MLRL_Cp_cm
Nottingham
Martin Broadley, Catherine Thomas
PhomaLS_Cou_pct
University of Hertfordshire
Yongju Huang, John West
Sdd-toc_M_mgkg
University of York
Swen Langer, Lenka Havlickova
NA
0
VertWilt_E_0or1
University of Hertfordshire
Yongju Huang, John West
SdCampesterolDiunsat_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdCr_M_mgkg
Nottingham
Thomas Alcock, David Salt
SdWght_M_mg
John Innes Centre
Rachel Wells
NA
0
LfSe_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
stearicSeedCont_M_pct
Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC
INRA
Anne Laperche
bioticAnnot_an_text
Biotic Stress;Biotic attack during the crop cycle;BIOTIC
INRA
Anne Laperche
60-90%
3
26-50% girdling
3
SdAlcII_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
LfSr_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
high damage
7
Diseased leaved with a few large lesions of many small leasions, area quite heavily infected
4
Sclerot_Cou_plnt6plnt
white blight
University of Hertfordshire
Yongju Huang, John West
3
SdAvenasterol_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdCd_M_mgkg
Nottingham
Thomas Alcock, David Salt
4
regular
7
seedImpur_M_pct
Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR
INRA
Anne Laperche
SNi_M_mgkg
Nottingham
Thomas Alcock, David Salt
LfAs_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
No disease observable
0
Evidence of stunting
6
irregular
3
Half green/half diseased, leaf curling and distortion
5
4
Brassica ontology
Brassica napus ontology
Trace of disease
1
acronym
very low damage
2
SdB_M_mgkg
Nottingham
Thomas Alcock, David Salt
Average loding
5
NA
0
moderately regular
5
6
Plants heavily stunted or dead.
7
intermediate damage
5
entity
low to intermediate damage
4
low damage
3
no visible affection
0
attribute
Presence (1) or absence (0) of powdery mildew
Absence or presence of powdery mildew
Plant
Powdery mildew presence
Lateral root number
Number of lateral roots
LRN
Lateral root
number
Oil seed content NIRS - Measurement
NIRS assay. Pct of Seed Dry Weight
Seed Chromium content
The concentration of Chromium in seeds
SdCr
Seed
Chromium content
Seed weight - Measurement
Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser.
Leaf Strontium content
The concentration of Strontium in leaves
LfSr
Leaf
Strontium content
Seed number
Number of seeds in ten typical pods
seed
number
Regularity of plant stand
Scoring of the regularity of the plant stand
Plant
Stand
GLS seed content NIRS - Measurement
NIRS assay Quantity per Seed Dry Weight
Seed Cycloartenol content
The percentage of Cycloartenol in the total of Phytosterols in seeds
Cycloartenol
SdCycloartenol_Cp
Seed
Cycloartenol proportion of Phytosterols
Seed Molybdenum content
The concentration of Molybdenum in seeds
SdMo
Seed
Molybdenum content
Seed B-Sitosterol content
The concentration of B-Sitosterol in seeds
B-Sitosterol
SdB-Sitosterol
Seed
B-Sitosterol content
GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement
* 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.).
* Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers.
* Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C.
* Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C.
* After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS.
* GC program:
* Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes.
* Inlet temp: 280°C, split-less injection.
* Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min.
* FID temp: 325°C
Seed Cadmium content
The concentration of Cadmium in seeds
SdCd
Seed
Cadmium content
Seed delta- tocopherol content
Delta- tocopherol content in seeds
Sdd-toc
seed
delta-tocopherol content
Seed Zinc content
The concentration of Zinc in seeds
SdZn
Seed
Zinc content
Biomass yield
Weight (kg) of raw materials harvested by plot
Biomass
Yield
Seed Sulphur content
The concentration of Sulphur in seeds
SdS
Seed
Sulphur content
Leaf mineral analysis - Measurement
3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry
Seed Iron content
The concentration of Iron in seeds
SdFe
Seed
Iron content
First flowers time (BBCH 60)
First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale)
BBCH growth stage 60;CETIUM growth stage F1
Plant
Development stage
Seed Strontium content
The concentration of Strontium in seeds
SdSr
Seed
Strontium content
Leaf Sulphur content
The concentration of Sulphur in leaves
LfS
Leaf
Sulphur content
Pod width - Measurement
Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged
Seed Avenasterol content
The percentage of Avenasterol in the total of Phytosterols in seeds
Avenasterol
SdAvenasterol_Cp
Seed
Avenasterol proportion of Phytosterols
seed
Number of plants with phoma leaf spots
The number of plants that showed presence of phoma leaf spots from a subsample of a plot
Plant
Phoma leaf spots
Leaf Calcium content
The concentration of Calcium in leaves
LfCa
Leaf
Calcium content
Nitrate Harvest index - Computation
Samples from HI assay are analysed for N_%DW in the different fractions
Seed sieving - Estimation
Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter.
Seed erucic acid content
The concentration of erucic acid in seeds.
SdErucic
Seed
Erucid acid content
Leaf Molybdenum content
The concentration of Molybdenum in leaves
LfMo
Leaf
Molybdenum content
Seed linolenic acid content
The concentration of linolenic acid in seeds.
StLinolenic
Seed
Linolenic acid content
Seed impurity content – Computation
Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively
mg/kg
Plot measure validity – Estimation
Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid”
Plot measure validity
Assessment on measure validity.
Valid
Plot
Measure validity
Phoma leaf spot severity
Severity assessment of phoma leaf spot
Leaf
Leaf phoma resistance
Phoma stem cancer severity - Estimation
Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged))
cm
Seed moisture NIRS - Measurement
NIRS assay
LLS severity on stem (percentage of stem area covered by LLS)
Light leaf spot severity on stems, judged by the percentage of area covered by LLS.
Stem
Light leaf spot severity
Pod and stem Ketone functional group content
The concentration of components with ketone functional groups from pod and stem material
SdKeto
Pod and stem
Ketone functional group content
Stem length - Measurement
Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing.
0-9 Damage/Incidence scale
Early pod development time (BBCH 73)
30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale)
BBCH growth stage 73;CETIUM growth stage G4
Plant
Development stage
Leaf Uranium content
The concentration of Uranium in leaves
LfU
Leaf
Uranium content
Seed glucosinolate content
The concentration of glucosinolates in seeds.
SdGLS
Seed
Glucosinolate content
%
Seed protein yield
Protein yield per ha
PrY
Seed
Protein yield
Seed Sodium content
The concentration of Sodium in seeds
SdNa
Seed
Sodium content
Phoma incidence (diseased) - Counting
10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot.
0-7 disease stage scale
Seed Avenasterol content
The concentration of Avenasterol in seeds
Avenasterol
SdAvenasterol
Seed
Avenasterol content
Seed mineral analysis - Measurement
3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg
Beginning of pod maturity time (BBCH 81)
10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale)
BBCH growth stage 81;CETIUM growth stage G5
Plant
Development stage
Seed Calcium content
The concentration of Calcium in seeds
SdCa
Seed
Calcium content
Additionnal pod maturity stages (BBCH 82-99) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Protein seed content NIRS - Measurement
NIRS assay. Pct of Seed Dry Weight
Seed Boron content
The concentration of Boron in seeds
SdB
Seed
Boron content
Leaf miner incidence (diseased) - Counting
10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner.
Stem witdh - Measurement
Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image.
LLS severity - Estimation
10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated.
Plant height - Measurement
Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing.
Pod shattering - Estimation
Visual notations (scale 0 - 9)
First inter-node time (BBCH 31)
Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale)
BBCH growth stage 31;CETIUM growth stage C1
Plant
Development stage
Beginning of flowering time (BBCH 61)
10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale)
BBCH growth stage 61;CETIUM growth stage F2
Plant
Development stage
day-of-year
Seed total Phytosterol content
The total concentration of phytosterols in seeds
SdPhytosterol
Seed
Phytosterol content
Sclerotinia incidence (diseased) - Counting
Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate).
Linoleic seed content NIRS - Measurement
NIRS assay. Pct total fatty acids
Seed Phosphorus content
The concentration of Phosphorus in seeds
dSP
Seed
Phosphorus content
LLS severity - Estimation
10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated.
Primary root lengh
Length of the primary root
PRL
Primary root
length
Seed Cobalt content
The concentration of Cobalt in seeds
SdCo
Seed
Cobalt content
Comment
Leaf Silver content
The concentration of Silver in leaves
LfAg
Leaf
Silver content
Beginning of pod maturity (BBCH 81) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
HailDmg - Estimation
Score the damage caused by hail.
Seed Nickel content
The concentration of Nickel in seeds
SdNi
Seed
Nickel content
plotharvest - Measurement
A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester
Seed diunsaturated Campesterol content
The concentration of diunsaturated Campesterol in seeds
Campesterol diunsat
SdCampesterolDiunsat
Seed
diunsaturated Campesterol content
Beginning of flowering (BBCH 61) date - Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Seed Rubidium content
The concentration of Rubidium in seeds
SdRb
Seed
Rubidium content
LLS incidence on leaf
The percentage of plants that showed presence of light leaf spot incidence on leaves
Leaf
Light leaf spot incidence
Seed oil content
The concentration of oil in seeds.
SdOil
Seed
Oil content
Seed linoleic acid content
The concentration of linoleic acid in seeds.
StLinoleic
Seed
Linoleic acid content
Seed B-Sitosterol content
The percentage of B-Sitosterol in the total of Phytosterols in seeds
B-Sitosterol
SdB-Sitosterol_Cp
Seed
B-Sitosterol proportion of Phytosterols
Seed alpha- tocopherol content
Alpha- tocopherol content in seeds
Sda-toc
seed
alpha-tocopherol content
Climatic incidents annotions - Estimation
Visual observations
Seed Magnesium content
The concentration of Magnesium in seeds
SdMg
Seed
Magnesium content
Total root length
Length of all roots
TRL
Total root
length
Leaf Lead content
The concentration of Lead in leaves
LfPb
Leaf
Lead content
Seed diunsaturated Campesterol content
The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds
Campesterol diunsat
SdCampesterolDiunsat_Cp
Seed
Campesterol diunsat proportion of Phytosterols
Seed moisture content
Seed moisture content.
SdMoist
Seed
Moisture content
Image analysis - Counting
RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN).
GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement
* Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation.
* Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step.
* 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples.
* Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour.
* Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials.
* A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions.
* The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes.
* 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used).
* The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane.
* Samples are vortexed, centrifuged and the upper hexane phase is removed.
* A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first.
* Samples are dried in a rotary evaporator or under a stream of nitrogen.
* Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID).
*GC program:
* Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes.
* Inlet temp: 325°C, split-less injection.
* Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min.
* FID temp: 325°C
Plant lodging incidence
The plant lodging incidence.
Lodg
Plant
Lodging incidence
Seed moisture content – Computation
Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively
Beginning of pod development (BBCH 71) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
root/cm
Palmitic seed content NIRS - Measurement
NIRS assay. Pct total fatty acids
Phoma stem canker upper stem lesion score
Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa
Upper stem
Phoma stem cancer severity
Pod and stemprimary Alkohol functional group content
The concentration of components with primary alcohol functional groups from pod and stem material
SdAlcI
Pod and stem
primary Alkohol functional group content
Pod and stem wax content
The concentration of wax in the pod and seed material
SdTotalWax
Pod and stem
wax content
Seed Cycloartenol content
The concentration of Cycloartenol in seeds
Cycloartenol
SdCycloartenol
Seed
Cycloartenol content
Additionnal pod development stages (BBCH 72-79) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Seed N content to total plant N content ratio
Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root)
Nitrogen Harvest Index
NHI
Seed
N content proportion of plant N content
Seed Campesterol content
The concentration of Campesterol in seeds
Campesterol
SdCampesterol
Seed
Campesterol content
Plant height
Maximum plant height from ground to tip of terminal raceme
PH
plant
height
Seed protein Yield - Computation
Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr)
Leaf Magnesium content
The concentration of Magnesium in leaves
LfMg
Leaf
Magnesium content
Seed Campesterol content
The percentage of Campesterol in the total of Phytosterols in seeds
Campesterol
SdCampesterol_Cp
Seed
Campesterol proportion of Phytosterols
Seed impurity content
Percentage of impurities in a sample of seeds determined by weighing.
SdImpur
Seed
Impurities content
Pod number
Number of pods on terminal raceme
pod
number
Seed stearic acid content
The concentration of stearic acid in seeds.
SdStearic
Seed
Stearic acid content
Biotic attack annotation
Biotic attack during the crop cycle.
BioticPb
Plant
Exposure to biotic attack; damage du to biotic attack
Beginning of pod development time (BBCH 71)
10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale)
BBCH growth stage 71;CETIUM growth stage G1
Plant
Development stage
Weevil damage - Estimation
Observe damage caused by Weevils (all species), and rate the damage.
Leaf Cadmium content
The concentration of Cadmium in leaves
LfCd
Leaf
Cadmium content
Leaf Titanium content
The concentration of Titanium in leaves
LfTi
Leaf
Titanium content
HPLC (High performance liquid chromatography) analysis - Measurement
A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK)
twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK)
dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis.
Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents
Lateral root density
The mean density of lateral roots along the primary root
LRD
Lateral root
density
Seed Brassicasterol content
The percentage of Brassicasterol in the total of Phytosterols in seeds
Brassicasterol
SdBrassicasterol
Seed
Brassicasterol proportion of Phytosterols
Pod development time (BBCH 72-79)
Additional stages for pod development according to the BBCH scale (72-79)
pod development stage;time to BBCH growth stages 72-79
Plant
Development stage
variable_of
Phoma damage – G2 score – Computation
Evaluation of the Phoma lingam infection with the G2 score.
The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation.
Leaf Iron content
The concentration of Iron in leaves
LfFe
Leaf
Iron content
Image analysis - Computation
The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001)
Phoma stem cancer severity - Estimation
Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged))
Fertile Branches - Counting
Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing.
Pedicel length - Measurement
Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged
Leaf phoma resistance - Estimation
10 spots per plant]]>
Verticilium wilt presence - Estimation
Scoring of presence or absence of verticilium wilt symptoms across whole plant.
Seed Manganese content
The concentration of Manganese in seeds
SdMn
Seed
Manganese content
Crop problems annotations - Estimation
Visual observations
mg
Foot length
Length of lower stem from ground level to last formed fertile branch
foot
length
Erucid seed content NIRS - Measurement
NIRS assay. Pct total fatty acids
Hail damage
Plant damage caused by hail.
HailDmg
Plant
Hail damage
Seed weight
Seed weight of ten typical pods
seed
weight
Pod and stem branched Alcyl-chain content
The concentration of all compounds with branched alcyl chains from pod and stem material
SdBranched
Pod and stem
branched Alcyl-chain content
Leaf Chromium content
The concentration of Chromium in leaves
LfCr
Leaf
Chromium content
Stearic seed content NIRS - Measurement
NIRS assay. Pct total fatty acids
Oil yield - Computation
Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O)
Seed Copper content
The concentration of Copper in seeds
SdCu
Seed
Copper content
Pod and stem Aldehyde functional group content
The concentration of components with aldehyde functional groups from pod and stem material
SdAld
Pod and stem
Aldehyde functional group content
Pod and stem secondary Alkohol functional group content
The concentration of components with secondary alcohol functional groups from pod and stem material
SdAlcII
Pod and stem
secondary Alkohol functional group content
Seed moisture content
Seed moisture content.
SdMoist
Seed
Moisture content
kg
Pod shattering
Proportion of open pods (assessment)
PodShat
Pod
Shattering
Plant stand at emergence – Computation
EMERG = (Number of plants obtained / Number of seeds sown) x 100
LLS severity on leaf
Light Leaf spot severity on leafs
Leaf
Light leaf spot resistance
Pedicel length
Pedicel length of pods from terminal raceme
pedicel
length
Relative seed Phytosterol content - Computation
The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols
Leaf Caesium content
The concentration of Caesium in leaves
LfCs
Leaf
Caesium content
Pod length - Measurement
Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged
Plot Size – Computation
Surface is determined by the experimental design
Verticillium wilt incidence
The percentage of plants that showed presence of leaf miner from a subsample of a plot
Plant
Verticilium wilt presence
boolean
Pod and stem Fatty acids functional group content
The concentration of components with fatty acids as functional groups from pod and stem material
SdFA
Pod and stem
Fatty acids functional group content
0-7 percentage scale
Leaf Phosphorus content
The concentration of Phosphorus in leaves
LfP
Leaf
Phosphorus content
Plant stand
Number of viable plants counted per surface unit
Crop stand
Plant density
PltSd
Plant
Stand
Biotic stress annotations - Estimation
Visual observations
Leaf Boron content
The concentration of Boron in leaves
LfB
Leaf
Boron content
Linolenic seed content NIRS - Measurement
NIRS assay. Pct total fatty acids
Crop problems annotation
Crop problems during the crop cycle.
CropPb
Plant
Exposure to crop problems;damage from crop problem
Pod length
Pod length of pods from terminal raceme
pod
length
Phoma stem canker base stem score
Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa
Stem base
Phoma stem cancer severity
Verticilium wilt presence
Absence or presence of Verticilium wilt on the plant
Plant
Verticilium wilt presence
Mean lateral root length
The mean length of a lateral root of that root system
MLRL
Lateral root
average length
0-3 disease stage scale
Seed Sigmasterol content
The concentration of Stigmasterol in seeds
Stigmasterol
SdStigmasterol
Seed
Stigmasterol content
Leaf Potassium content
The concentration of Potassium in leaves
LfK
Leaf
Potassium content
Seed Arsenic content
The concentration of Arsenic in seeds
SdAs
Seed
Arsenic content
Leaf Zinc content
The concentration of Zinc in leaves
LfZn
Leaf
Zinc content
Leaf Nickel content
The concentration of Nickel in leaves
LfNi
Leaf
Nickel content
Image analysis - Computation
The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001)
Pod and stem Alkane-functional group content
The concentration of components with alkane functional groups from pod and stem material
SdAlk
Pod and stem
Alkane-functional group content
Incidence of Phoma leaf spot
The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot
Leaf
Phoma incidence
Seed Potassium content
The concentration of Potassium in seeds
SdK
Seed
Potassium content
Seed palmitic acid content
The concentration of palmitic acid in seeds.
SdPalmitic
Seed
Palmitic acid content
Leaf Manganese content
The concentration of Manganese in leaves
LfMn
Leaf
Manganese content
Lateral root length
Length of the lateral root
LRL
Lateral root
length
Seed protein content
The concentration of proteins in seeds.
SdProt
Seed
Protein content
Seed Selenium content
The concentration of Selenium in seeds
SdSe
Seed
Selenium content
mm
No of plants with Sclerotinia (out of six)
Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering.
Plant
Sclerotinia incidence
LLS severity on pods (percentage of pod area covered by LLS)
Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS.
Pod
Light leaf spot severity
Leaf Rubidium content
The concentration of Rubidium in leaves
LfRb
Leaf
Rubidium content
Oleic seed content NIRS - Measurement
NIRS assay. Pct total fatty acids
Verticilium wilt presence - Counting
Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded.
Leaf Selenium content
The concentration of Selenium in leaves
LfSe
Leaf
Selenium content
End of flowering time (BBCH 69)
End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale)
BBCH growth stage 69;end flowering stage
Plant
Development stage
Oil yield
Oil yield
OY
Plant
Oil yield
Pod width
Pod width of pods from terminal raceme
pod
width
LLS incidence (diseased) - Estimation
10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%.
Number of fertile branches
Number of fertile branches
fertile branch
number
Phoma stem cancer severity - Estimation
Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged))
Flowering time (BBCH 62-65)
Additional flowering stages according to the BBCH scale (62-65)
flowering stage;time to BBCH growth stage 62-65;Anthesis
Plant
Development stage
Leaf Sodium content
The concentration of Sodium in leaves
LfNa
Leaf
Sodium content
Seed oleic acid content
The concentration of oleic acid in seeds.
StOleic
Seed
Oleic acid content
Leaf Aluminium content
The concentration of Aluminium in leaves
LfAl
Leaf
Aluminium content
Seed Number - Counting
Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser.
Phoma leaf spot incidence (diseased) - Estimation
10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded
Leaf Arsenic content
The concentration of Arsenic in leaves
LfAs
Leaf
Arsenic content
0-5 percentage bracket scale
2020-10-19T00:00:02
pod/20cm of terminal raceme
0-9 Lodging Incidence scale
Seed Lead content
The concentration of Lead in seeds
SdPb
Seed
Lead content
Protein meal content NIRS - Measurement
NIRS assay. Pct of Seed Dry Weight
Leaf Balium content
The concentration of Balium in leaves
LfBa
Leaf
Balium content
Addictional flowering stages (BBCH 62-65) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Stem width
Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant
stem
width
Early pod development (BBCH 73) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Incidence with leaf miner
The percentage of plants that showed presence of leaf miner from a subsample of a plot
Leaf
Leaf miner incidence
q/ha
Seed gamma- tocopherol content
Gamma- tocopherol conten in seeds
Sdy-toc
seed
gamma-tocopherol content
No of plants with Sclerotinia (per plot)
Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering.
Plant
Sclerotinia incidence
First flowers (BBCH 60) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Plant lodging incidence - Estimation
Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging
Climatic incidents annotation
Climatic incidents during the crop cycle.
ClimPb
Plant
Exposure to climatic stress
Plot size
Surface of the elementary experimental plot
PlotSize
Plot
Size
End of flowering (BBCH 69) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Seed Sigmasterol content
The percentage of Stigmasterol in the total of Phytosterols in seeds
Stigmasterol
SdStigmasterol_Cp
Seed
Stigmasterol proportion of Phytosterols
Sclerotinia incidence (diseased) - Counting
The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded.
Powdery mildew presence - Estimation
Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1
Seed Brassicasterol content
The concentration of Brassicasterol in seeds
Brassicasterol
SdBrassicasterol
Seed
Brassicasterol content
Plant stand at emergence
Percentage of seeds that emerge.
PltSdEm
Seed
Plant emergence
Image analysis - Computation
Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001)
Plant stand - Counting
Count the number of plants on linear sections defined in each sowing line at the exception of the border lines
Leaf Copper content
The concentration of Copper in leaves
LfCu
Leaf
Copper content
Seed Lithium content
The concentration of Lithium in seeds
SdLi
Seed
Lithium content
0-9 Regularity scale
Pod beak length - Measurement
Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant.
Phoma damage
Withering disease caused by the agent Leptosphaeria maculans (Phoma).
Withering disease
PhomaDmg
Plant
Phoma damage
μmol/g
Pod maturity time (BBCH 82-99)
Additional stages for pod maturity according to the BBCH scale (82-99)
pod maturity stage;time to BBCH growth stage 82-99
Plant
Development stage
Phoma stem canker severity
Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa
Stem
Phoma stem cancer severity
Weevils damage
Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil).
WeevilsDmg
Plant
Weevils damage
Seed moisture content humidifier - Measurement
Humidimeter (sample of seeds)
plants/10 plants
root
Seed diameter
The diameter of a seed
SD
Seed
diameter
Plant height - Measurement
Height is measured from ground to the top of the plants using a dipstick graduated in cm
method_of
plants/6 plants
Meal protein content
The concentration of proteins in the meal.
MlProt
Meal
Protein content
Pod Number - Counting
Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing.
LLS resistance - Estimation
10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7.
Leaf Vanadium content
The concentration of Vanadium in leaves
LfV
Leaf
Vanadium content
fertile branch
Beak length
Pod beak length
beak
length
First inter-node (BBCH 31) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
plant/m2
plants/plot
regularity stand - Estimation
Visual notations (scale 0 - 9)
scale_of