6 medium green MatHardT_Meas_0to10 NARO, Uganda Elizabeth Kakhasa Matooke hardness in hand measurement scale 0-10 Rachel Chase Bioversity International MlBdShd_Comp_1to3 KU Leuven rwcPlant Sebastien Carpentier rwcPlant_Comp_unitless plant_rwc Bioversity International HndNbWhlBnch_Ct_hands Rachel Chase Rachel Chase Bioversity International CmpdTplMnCl_Est_1to6 IITA-BTI FngUnSmshdPlpSmth_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) GdBnch_Est_1to2 Allan Brown IITA PratyGooNumSlWt_Comp_Nema Danny Coyne IITA KU Leuven root_rwc Sebastien Carpentier rwcRoot_Comp_unitless rwcRoot Rhiannon Crichton Bioversity International temp30 5 Other Bioversity International PStmDiscol_Est_cm Inge Van den Bergh FrtApxFlwRlt_Est_1to4 Rachel Chase Bioversity International 3 highly imbricate Rhiannon Crichton AvgFrtDiam_M_mm Bioversity International MatAro_Meas_0to10 Elizabeth Kakhasa NARO, Uganda Matooke aroma measurement scale 0-10 HndMltpFrtNum_Ct_Frt Rhiannon Crichton Bioversity International Rachel Chase Bioversity International MgnBhvPtCnlThrdLf_Est_1to5 10 dont know IITA Tendo Ssali WvlLvPrTrp_Ct_weevil soft 0 PA_EstResp_Txt Bioversity Noel Madalla 4 margins overlapping Selection criteria for genotype that was ranked second lowest by female respondents during preference scoring exercise Bioversity Noel Madalla Rhiannon Crichton Bioversity International AcAnYd_Comp_TnHaY KU Leuven Sebastien Carpentier singleLeafArea_Meas_cm2 areaPerLeaf areaSingleLeaf RadoNumRtWt2_Comp_Nema Danny Coyne IITA temp44 IITA Tendo Ssali AnthrCl_Est_1to8 Rachel Chase Bioversity International FrtGrth_M_cm Guillaume Bauchet IITA-BTI IITA-BTI Allan Brown/Guillaume Bauchet (temp.) FngSmshdPlpArm_Est_1to5 3 equal to or more than 17 PlntShtTime_Comp_d Bioversity International Rhiannon Crichton 3 juvenile waterLossSystem waterLossSyst_Meas_ml KU Leuven Sebastien Carpentier transpSyst Rachel Chase BrctBhvBfFll_Est_1to2 Bioversity International IITA Tendo Ssali WvlAdltHdCps_M_µM IITA-Bioversity International BlkLfStrkSvrt_Est_MedLawCdsd 7 pink-purple Bioversity International Rhiannon Crichton PltDead_Ct_Plnt Bioversity International BlkLfStrkSvrtLfNb_Ct_grd3 Rhiannon Crichton Rhiannon Crichton FrtExtLng_M_mm Bioversity International rootShootDry_Comp_unitless KU Leuven Sebastien Carpentier rootShootRatioDry massDryRootShoot Rhiannon Crichton FngWtTtl_Comp_kg Bioversity International Rachel Chase Bioversity International BnchShp_Estm_1to5 1 winged and undulating high intensity 10 Tendo Ssali AvgWvlAdltBdLgt_M_µM IITA Rhiannon Crichton OdStdLfRk_Ct_leaf Bioversity International Other 7 Selection criteria for genotype that was ranked third lowest by female respondents during preference scoring exercise Bioversity Noel Madalla PulpDiam_M_mm Rhiannon Crichton Bioversity International whitish 9 without any floral relicts 1 BnchPst_Estm_1to5 Rachel Chase Bioversity International Bioversity International HandWt_M_kgPlnt Rhiannon Crichton other 5 moderately imbricate 2 leafAreaIncrease_Comp_cm2 KU Leuven Sebastien Carpentier leafAreaIncrease IITA Tendo Ssali TrpNum_Ct_weeviltrap persistent style 2 Rhiannon Crichton Bioversity International BnchFrtNum_Comp_Frt Bioversity Noel Madalla Selection criteria for genotype that was ranked second lowest by male respondents during preference scoring exercise Bioversity International Rhiannon Crichton BlkLfStrkLfFrStg6_Date_dmy Guillaume Bauchet IITA-BTI PulpDiam_M_cm green 1 Rhiannon Crichton Bioversity International AvgPeelThck_M_mm Bioversity International temp32 Rhiannon Crichton Rhiannon Crichton Bioversity International temp40 Rachel Chase Bioversity International CigLfPigm_Est_1to3 small blotches 2 HvstDth_Comp_d Rhiannon Crichton Bioversity International PStmHt_M1_cm Bioversity International Rhiannon Crichton TE_dry KU Leuven Sebastien Carpentier TEtotalDry transpirationEfficiency_Comp_gml Rhiannon Crichton NtHvstPrp_Comp_PlntNb Bioversity International LfChgPres_Est_1or2 Inge Van den Bergh Bioversity International IITA-BTI Allan Brown/Guillaume Bauchet (temp.) FngSmshdPlpSmth_Est_1to5 IITA-BTI Guillaume Bauchet Shoot_Date_ymd HndFrtAvgNum_Ct_Frt Bioversity International Rhiannon Crichton 5 bright yellow Rhiannon Crichton Bioversity International SptPsdStmPercFW_Comp_pct IITA-Bioversity International SpotLfNum_Ct_Lf Amos Alakonya-Rhiannon Crichton IITA Tendo Ssali AvgWvlAdltBdWght_M_mg relativeRoot_dry rootToPlantMassRatio_Comp_unitless KU Leuven massDryratioRoot Sebastien Carpentier MSuckN_Ct_MSuck Rhiannon Crichton Bioversity International CIRAD MlRchsTp_Estm_1to2 Christophe Jenny Bioversity International/IITA Inge Van den Bergh/Danny Coyne temp47 Rachel Chase Bioversity International BrctImbrcApxMlBd_Est_1to3 Inge Van den Bergh PlntgDisTim_Comp_d Bioversity International Bioversity International DateDeath_Est_dmy Rhiannon Crichton falling vertically 1 13-16 2 Rhiannon Crichton Bioversity International RkPvsMktLf_Comp_leafrank IITA PdltFrt_Est_1to2 Allan Brown Rachel Chase Bioversity International MlRchsPst_Estm_1to5 PMargCol_Esr_1to16 Rachel Chase Bioversity International Rhiannon Crichton Bioversity International DateColl_Date_dmy 0 no intensity 1 cream 1 trace infections with a few small galls Allan Brown/Guillaume Bauchet (temp.) IITA-BTI FngUnSmshdPlpCl_Est_1to5 temp50 Inge Van den Bergh/Danny Coyne Bioversity International/IITA temp13 Rhiannon Crichton Bioversity International IITA Tendo Ssali WvlLvBdWght_M_mg 2 medium (0.45 < w/y < 0.55) Guillaume Bauchet DateHarv_Date_ymd IITA-BTI pink-purple to red 2 4 First-spot stage: the streaks broaden and become more or less fusiform or elliptical in outline and a water soaked border appears around each lesion. Tendo Ssali IITA AvgWvlAdltHdCps_M_µM Guillaume Bauchet DamEst_Date_ymd IITA-BTI Danny Coyne HoploNumRtWt2_Comp_Nema IITA Bioversity International BlotchA_Est_1to5 Rachel Chase waterLossSystem_per_Area Sebastien Carpentier waterLossSystPerLeafArea_Meas_mlcm2 KU Leuven Bioversity International PStmGrth_M_cm Rhiannon Crichton Sebastien Carpentier growthPS_per_leafFormed pseudostemHeightIncreasePerFormedLeaf_Comp_cm KU Leuven Bioversity International Rhiannon Crichton PltDth_Comp_d Rhiannon Crichton FrtDiam_M_mm Bioversity International Rhiannon Crichton FlwHvstTime_Comp_d Bioversity International LfEmRate_Comp_LfWk Rhiannon Crichton Bioversity International IITA-Bioversity International BlkLfStrkSvrt_Est_pct Rachel Chase Bioversity International PsdHght_E Bioversity International Rhiannon Crichton FrtNb_Ct_gFruit HndRnk_Ct_HndRnk Rhiannon Crichton Bioversity International Bioversity International Rhiannon Crichton temp11 1 like a top 3 yellow Bioversity International PltSpcg_Comp_m2 Rhiannon Crichton Rhiannon Crichton PSuckN_Ct_PSuck Bioversity International 1 hanging vertically AnthrCl_Est_1to16 Rachel Chase Bioversity International Rachel Chase Bioversity International BrctApxShp_Est_1to5 Guillaume Bauchet FlwT_Date_ymd IITA-BTI IITA Danny Coyne MeloiNumSlWt_Comp_Nema Inge Van den Bergh PStmSplitPres_Est_1or2 Bioversity International Rhiannon Crichton HvstDst_Comp_PlntHa Bioversity International WvlAdltBdLgt_M_µM IITA Tendo Ssali 1 convolute no internal symptoms 1 temp2 Bioversity International Rhiannon Crichton contrast between margin and petiole (with a contrasting colour line along) 2 horizontal or supra-horizontal 4 0 crumbly 2 medium shouldered (0.28 < x/y < 0.30) areaLeaves areaTotLeaves KU Leuven Sebastien Carpentier totLeafArea_Meas_cm2 Allan Brown/Guillaume Bauchet (temp.) IITA-BTI FngUnSmshdPlpTxt_Est_1to5 1 dislike very much DeathCause_Est Rhiannon Crichton Bioversity International > 100 egg masses 5 Rachel Chase Bioversity International CmpdTplLbCl_Est_1to5 3 34-66 % of older leaves turning yellow, with some hanging down the pseudostem green 2 NARO, Uganda Matooke pumpkin aroma measurement 2pt scale MatPump_Meas_2pt Elizabeth Kakhasa Bioversity International Rhiannon Crichton temp12 2 winged and not clasping the pseudostem Rhiannon Crichton Bioversity International NtHvstDst_Comp_PlntHa Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd0 Bioversity International NARO, Uganda Elizabeth Kakhasa MatGrass_Meas_2pt Matooke grassy aroma measurement 2pt scale KU Leuven Sebastien Carpentier rootFreshMass_Meas_g root_fresh massFreshRoot 3 winged and clasping the pseudostem obtuse 4 less than 1% of lamina with symptoms, only streaks and/or up to 10 spots 1 PtlClpFW_Comp_pct Bioversity International Rhiannon Crichton Inge Van den Bergh/Danny Coyne temp49 Bioversity International/IITA total discolouration of vascular tissue 6 BnchMatStg_Est_1to2 IITA-BTI Guillaume Bauchet Matooke surface yellowness measurement scale 0-10 MatSurfYellV_Meas_0to10 NARO, Uganda Elizabeth Kakhasa 0 soft Danny Coyne PratyCofNumSlWt_Comp_Nema IITA PratyNumSlWt_Comp_Nema IITA Danny Coyne growthDry growthDry_Comp_g Sebastien Carpentier plantGrowth_dry KU Leuven Third or mature spot stage: the centre of the spot dries out, becoming light grey or buff-coloured, and further depressed. The spot is surrounded by a narrow, well-defined, dark brown or black border. between the latter and the normal green colour of the leaf, there is often a bright yellow transitional zone. After the leaf has collapsed and withered, spots remain clearly visible because of the light-coloured centre and dark border. 6 2 fusarium wilt BoiPlantnStic_Meas_0to10 Hermann Antonin KOUASSI Boiled plantain Stickiness scale 0-10 CIRAD / UNA, Côte d’Ivoire Allan Brown IITA GdFrtFll_Est_1to2 5 other IITA Tendo Ssali WvlLv_Ct_weevil Bioversity International Rhiannon Crichton RkYgstLf_Comp_leafrank temp15 Bioversity International Rhiannon Crichton Rachel Chase Bioversity International BrctClExtFc_Int_1to8 4 green yellow 0-33 % of older banana leaves turning yellow 2 Bioversity International/IITA Inge Van den Bergh/Danny Coyne temp51 Rachel Chase Bioversity International MlBdLngthRt_M_1to3 Rhiannon Crichton PeelThck_M_mm Bioversity International Noel Madalla PS_CompMale_PrefScale Bioversity IITA-Bioversity International SptLfNb_Ct_leaf Amos Alakonya-Rhiannon Crichton FstBrctApxShp_Est_1to4 Rachel Chase Bioversity International PStmHt_M2_cm Rhiannon Crichton Bioversity International 2 not winged Danny Coyne IITA RadoNumSlWt_Comp_Nema Selection criteria for genotype that was ranked highest by female respondents during preference scoring exercise Bioversity Noel Madalla discolouration greater than two-thirds of vascular tissue 5 Bioversity International YellowPres_Est_1or2 Inge Van den Bergh 10 orange red Rachel Chase Bioversity International BrctClExtFc_Est_1to10 DateColl_Date_ymd Guillaume Bauchet IITA-BTI Matooke firmness in mouth measurement scale 0-10 MatFirmM_Meas_0to10 NARO, Uganda Elizabeth Kakhasa CrmIntDiscFW_Est_1to5 Bioversity International Inge Van den Bergh with a curve 3 1 no pigmentation temp18 Rhiannon Crichton Bioversity International 4 1/3-2/3 discoloured 1 cylindrical PltHvstd_Ct_Plnt Bioversity International Rhiannon Crichton CormDiscol_Est_1to6 Inge Van den Bergh Bioversity International Elizabeth Kakhasa Matooke moldability measurement scale 0-10 MatMolT_Meas_0to10 NARO, Uganda relativeLeaf_dry massDryratioLeaf KU Leuven Sebastien Carpentier leafToPlantMassRatio_Comp_unitless red-purple 9 Sebastien Carpentier area_topView areaPlantTop topLeafArea_Meas_cm2 KU Leuven widthPerLeaf KU Leuven Sebastien Carpentier widthSingleLeaf singleLeafWidth_Meas_cm Rachel Chase Bioversity International MgnBhvPtCnlThrdLf_Est_1to4 leavesFormedPerPseudostemGrowth_Comp_percm KU Leuven Sebastien Carpentier leaves_per_heightPS Allan Brown/Guillaume Bauchet (temp.) FngUnSmshdPlpTst_Est_1to5 IITA-BTI Elizabeth Kakhasa NARO, Uganda MatSmoothM_Meas_0to10 Matooke smoothness in hand measurement scale 0-10 IITA NmRpFctr_Ct_Nema Danny Coyne high intensity 10 Bioversity International FrtftPdclLgth_M_mm Rachel Chase High shouldered (x/y less than or equal to 0.28) 1 Bioversity International ChgNwLfPercFW_Comp_pct Rhiannon Crichton Bioversity International CmpdTplLbCl_Est_1to16 Rachel Chase Bioversity International/IITA NmNumRtWt Inge Van den Bergh/Danny Coyne Rhiannon Crichton RchsWt_M_kg Bioversity International Bioversity International/IITA Inge Van den Bergh/Danny Coyne temp53 temp46 Bioversity International/IITA Inge Van den Bergh/Danny Coyne lumpy 0 singleLeafLength_Meas_cm lengthSingleLeaf KU Leuven lengthPerLeaf Sebastien Carpentier Tendo Ssali IITA WvlLvBdLgt_M_µM Inge Van den Bergh Bioversity International CrmDiscDistTlstSckFW_M_cm SuckNum_Ct_Suck Rhiannon Crichton Bioversity International 2 isolated points of discolouration in vascular tissue Bioversity International Rhiannon Crichton WSuckN_Ct_WSuck 10 bright yellow IITA temp43 Tendo Ssali 34-50% of lamina with symptoms 5 8 red Bioversity International/IITA temp45 Inge Van den Bergh/Danny Coyne persistent flower relicts (> 20% of the fruits with relicts) 3 Guillaume Bauchet FrtIntLng_M_cm IITA-BTI Bioversity International HndFrtNum_Ct_Frt Rhiannon Crichton Stage 2 appears as a stripe, generally brown in colour and visible on the underside of the leaf, later the symptom also appears on the upper part of the limb as a stripe, the yellow colour of which resembles the stripe at stage 1 of Yellow Sigatoka. The colour of this stripe will change progressively from brown and later to black on the upper side of the limb, but will retain the brown colour on the underside. 2 Rhiannon Crichton Bioversity International AnPltPrp_Comp_KgHaY few flower relicts (< 20% of the fruits with relicts) 2 Bioversity International Rhiannon Crichton Shoot_Date_dmy other 4 FrtNbMdHndBnch_Ct_ft Rachel Chase Bioversity International numLeaves_Count_unitless totalNumLeaves numLeaf KU Leuven Sebastien Carpentier plant_fresh KU Leuven Sebastien Carpentier massFreshPlant plantFreshMass_Meas_g PMargCol_Esr_1to4 Rachel Chase Bioversity International BoiPlantnMeal_Meas_0to10 CIRAD / UNA, Côte d’Ivoire Hermann Antonin KOUASSI Boiled plantain Mealiness scale 0-10 FlwT_Date_dmy Rhiannon Crichton Bioversity International Matooke stickiness in hand measurement scale 0-10 MatStickT_Meas_0to10 NARO, Uganda Elizabeth Kakhasa temp42 Tendo Ssali IITA Bioversity International DthPrp_Comp_DdPltRt Rhiannon Crichton Inge Van den Bergh Bioversity International IntDisProp_Comp_pct margins overlapping 5 BlkLfStrkSvrt_Est_Faure IITA-Bioversity International Bioversity International DET_Comp_d Rhiannon Crichton BnchWt_M_kg Rhiannon Crichton Bioversity International Rhiannon Crichton Bioversity International BlkLfStrkSvrtLfNb_Ct_grd1 IITA Sckr_Est_1to2 Allan Brown NSpotLfProp_Comp_idx IITA-Bioversity International Inge Van den Bergh/Danny Coyne temp52 Bioversity International/IITA Rhiannon Crichton Bioversity International SSuckN_Ct_SSuck FrtftPdclLgth_Est_1to3 Rachel Chase Bioversity International temp20 Bioversity International Rhiannon Crichton Rhiannon Crichton Bioversity International BlkLfStrkInfIndex_Comp_Idx 10 moldable Rhiannon Crichton Bioversity International HvstPrp_Comp_PlntNb like very much 4 Rhiannon Crichton Bioversity International LfEmRate_Comp_LfMth straight in the distal part 3 weevils 6 IITA-BTI Guillaume Bauchet FrtDiam_M_cm BlkLfStrkLfFrStg1_Date_dmy Rhiannon Crichton Bioversity International Rachel Chase Bioversity International PdcFsn_Est_1to2 Matooke moisture in mouth measurement scale 0-10 MatMoistM_Meas_0to10 NARO, Uganda Elizabeth Kakhasa Rhiannon Crichton HndNumMsrd_Ct_Hnd Bioversity International Tendo Ssali WvlLvMsrd_Ct_weevil IITA leaf spots 1 Rachel Chase PStmCol_Est_1to16 Bioversity International CauDam_Est_txt Rhiannon Crichton Bioversity International long (equal to or more than 30 cm) 3 5 rounded 1-5% of lamina with symptoms 2 temp35 Bioversity International Rhiannon Crichton Rhiannon Crichton YlLfPercFW_Comp_pct Bioversity International like fairly 3 Danny Coyne HelicoNumSlWt_Comp_Nema IITA leaf_dry leafDryMass_Meas_g Sebastien Carpentier KU Leuven massDryLeaf DateDeath_Est_ymd IITA-BTI Guillaume Bauchet no symptoms 0 non sticky 0 KU Leuven lengthSum Sebastien Carpentier totLeafLength_Meas_cm IITA WvlAdltMsrd_Ct_weevil Tendo Ssali Boiled plantain Chewiness by number of chews CIRAD / UNA, Côte d’Ivoire Hermann Antonin KOUASSI BoiPlantnChew_Meas_number of chews 4 spiral (all fruit are attached to a unique crown coiled around the stalk) Bioversity International temp9 Rhiannon Crichton hard 10 2 3-10 egg masses 4 67-100 % of all leaves turning yellow and necrotic, with leaves hanging down the pseudostem IITA-BTI FngSmshdPlpCl_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) Bioversity International Rhiannon Crichton temp23 attribute 5 medium intensity Rhiannon Crichton FnctLeav_Ct_Leaf Bioversity International lanceolate 2 Small bunch from neutral/hermaphrodite flowers just above the male bud 6 waterLossDaily rateWaterLossPerDay waterLossDay_Comp_ml KU Leuven Sebastien Carpentier no contrast between margin and petiole (without a colour line along) 1 MlRchsApp_Estm_1to7 Rachel Chase Bioversity International BoiPlantnFirm_Meas_0to10 CIRAD / UNA, Côte d’Ivoire Hermann Antonin KOUASSI Boiled plantain Firmness scale 0-10 IITA PratyNumRtWt2_Comp_Nema Danny Coyne Rachel Chase Bioversity International BrctClExtFc_Int_1to16 6 entire inner rhizome discoloured Rachel Chase Bioversity International FrtApxPt_Est_1to5 > 1/3 discoloured 5 purple 6 Bioversity International Rhiannon Crichton temp22 2 16-20 cm Rhiannon Crichton Bioversity International BlkLfStrkSvrtLfNb_Ct_grd2 pink/pink-purple 10 totalNumFunctionalLeaves KU Leuven Sebastien Carpentier numFuncLeaf numFuncLeaves_Count_unitless medium intensity 5 PMargClasp_Est_1or2 Rachel Chase Bioversity International BlotchA_Est_1to4 Rachel Chase Bioversity International IITA-BTI FngSmshdPlpTxt_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) 4 margins curved inward 2 present BlotchCol_Est_1to4 Rachel Chase Bioversity International Bioversity International FrtWt_M_gFruit Rhiannon Crichton MatSour_Meas_0to10 NARO, Uganda Matooke sourness measurement scale 0-10 Elizabeth Kakhasa DthDnst_Comp_PlntHa Rhiannon Crichton Bioversity International Rhiannon Crichton AvgFrtExtLng_M_cm Bioversity International Danny Coyne IITA PratyGooNumRtWt2_Comp_Nema MlBdLngth_M_1to3 Rachel Chase Bioversity International Bioversity International AvgPulpDiam_M_mm Rhiannon Crichton 4 not winged and clasping the pseudostem 2 21-30 cm Inge Van den Bergh Bioversity International PStmSplitPres_Est_0or1 Tendo Ssali IITA WvlAdlt_Ct_weevil Bioversity International PetColpPres_Est_1or2 Inge Van den Bergh MatSweet_Meas_0to10 Matooke sweetness measurement scale 0-10 NARO, Uganda Elizabeth Kakhasa 0 no intensity Rhiannon Crichton Bioversity International FrtWtAvg_M_g cream 2 Bioversity International Rhiannon Crichton BlkLfStrkLfFrStg6_Date_ymd 6 Third or mature spot stage: the centres of the lesions dry out becoming light grey or buff coloured and a bright yellow transitional zone appears between them and the normal green colour of the leaf. The lesions remain clearly visible after the leaf has collapsed or withered because of their light coloured centre and dark border. Rhiannon Crichton temp21 Bioversity International winged 1 adult female 2 absent 0 2 not revolute (not rolling) Bioversity International RtnCrpCyc_Comp_d Rhiannon Crichton present 2 rwcLeaf_Comp_unitless leaf_rwc KU Leuven Sebastien Carpentier rwcLeaf Danny Coyne IITA RotyNumSlWt_CompNema large blotches 3 6 51-100% of lamina with symptoms StdLf_Ct_leaf IITA-Bioversity International Amos Alakonya-Rhiannon Crichton DmgIdxOut_M_% IITA Tendo Ssali Inge Van den Bergh PetColpPres_Est_0or1 Bioversity International Bioversity International MlBdShp_Comp_1to3 Rachel Chase Selection criteria for genotype that was ranked third highest by female respondents during preference scoring exercise Bioversity Noel Madalla Allan Brown/Guillaume Bauchet (temp.) IITA VitCCnt_M_ Bioversity Noel Madalla Selection criteria for genotype that was ranked third lowest by male respondents during preference scoring exercise clasping 1 whitish 9 Bioversity International FstYngLfsptd_Est_ymd Rhiannon Crichton yellow 4 Bioversity International Rhiannon Crichton BnchHndNum_Ct_Hnd 11-30 egg masses 3 FrtCvShp_Est_1to5 Rachel Chase Bioversity International Danny Coyne PratyCofNumRtWt2_Comp_Nema IITA Moist 10 bare 1 PltEst_Date_ymd IITA-BTI Guillaume Bauchet > 75 % of roots galled 5 2 at an angle 2 yellow areaPlant_per_areaTot leafAreaRatio_Comp_unitless areaRatio Sebastien Carpentier KU Leuven Inge Van den Bergh Bioversity International ExtDisProp_Comp_pct 10 orange red Allan Brown/Guillaume Bauchet (temp.) FngSmshdPlpTst_Est_1to5 IITA-BTI Rachel Chase Bioversity International CmpdTplMnCl_Est_1to16 KU Leuven Sebastien Carpentier heightPS heightPseudostem lengthPseudostem_Meas_cm 1 less than or equal to 20 cm HandAvgWt_M_kgPlnt Rhiannon Crichton Bioversity International 4 curved (sharp curve) 3 watery green 2 slightly curved 1 skinny (w/y less than or equal to 0.45) margins erect 2 3 watery green Guillaume Bauchet IITA-BTI OffTypEst_Date_ymd Bioversity International YellowPres_Est_0or1 Inge Van den Bergh 3 ivory Rachel Chase Bioversity International MlBdShp_Comp_1to5 pseudostemHeightIncrease_Comp_cm2 KU Leuven Sebastien Carpentier pseudostemHeightIncrease BoiPlantnSwt_Meas_0to10 CIRAD / UNA, Côte d’Ivoire Hermann Antonin KOUASSI Boiled plantain Sweetness scale 0-10 Rachel Chase Bioversity International PMargWing_Est_1or5 waterLossPlant transpPlant KU Leuven Sebastien Carpentier waterLossPlant_Meas_ml IITA HoploNumSlWt_Comp_Nema Danny Coyne straight in the distal part 2 IITA Dwfsm_Est_1to2 Allan Brown Tendo Ssali WvlLvHdCps_M_µM IITA 3 discolouration of up to one-third of vascular tissue 5 not winged and not clasping the pseudostem orange 6 4 Stage 4 appears on the underside as a brown spot and on the upper side as a black spot. Noel Madalla Selection criteria for genotype that was ranked highest by male respondents during preference scoring exercise Bioversity DmgPrphl_M_% Tendo Ssali IITA 1 yellow 2 slightly pointed IITA NmNumSlWt Danny Coyne equal to or more than 31 cm 5 lengthily pointed (like plantain) 2 KU Leuven Sebastien Carpentier growthFresh_Comp_g plantGrowth_fresh growthFresh pink/pink-purple 6 Bioversity International BlotchCol_Est_1to5 Rachel Chase Bioversity International BnchFrtNum_Ct_Frt Rhiannon Crichton Rachel Chase Bioversity International PMargColL_Est_1or2 FstExtDiSmptFW_Date_dmy Rhiannon Crichton Bioversity International 1 no visible sign of fusion Rhiannon Crichton Bioversity International SuckHtShtStg_M_cm 3 25-50 % of roots galled Bioversity International/IITA FuncRtNum2_Ct_Rt Inge Van den Bergh/Danny Coyne KU Leuven Sebastien Carpentier leafChlorosis_Comp_unitless leafChlorosis IITA MeloiNumRtWt2_Comp_Nema Danny Coyne Stage 6 is when the centre of the spot dries out, turns clear gray and is surrounded by a well-defined black ring, which is, in turn, surrounded by a bright yellow halo. These spots remain visible after the leaf has dried out because the ring persists. 6 Rhiannon Crichton temp19 Bioversity International 5 medium intensity 2 brown 3 equal to or more than 21 mm FrtExtLng_M_cm Rhiannon Crichton Bioversity International Rhiannon Crichton DIT_Comp_d Bioversity International Rhiannon Crichton CropCyc_Comp_d Bioversity International strongly bottle-necked (wider under tip than number 2) 4 neutral/male flowers on the whole stalk without persistent bracts (still firmly attached to the rachis) 5 5 like extremely pseudostemFreshMass_Meas_g Sebastien Carpentier massFreshPseudostem pseudostem_fresh KU Leuven fully green 1 Bioversity International temp16 Rhiannon Crichton pseudostemToPlantMassRatio_Comp_unitless massDryratioPseudostem KU Leuven Sebastien Carpentier relativePS_dry BlkLfStrkSvrt_Est_Gauhl IITA-Bioversity International Initial speck stage: faint, minute, reddish-brown specks on the lower surface of the leaf. 1 Bioversity International temp14 Rhiannon Crichton massDryRoot root_dry KU Leuven rootDryMass_Meas_g Sebastien Carpentier Selection criteria for genotype that was ranked second highest by male respondents during preference scoring exercise Bioversity Noel Madalla 3 other (specify on answer sheet) 3 moderate blotching (20-50%) nematodes 7 5 grainy intermeditae 2 Bioversity International BlkLfStrkSvrtLfNb_Ct_grd4 Rhiannon Crichton 1 pointed Rhiannon Crichton PltPltd_Ct_Plnt Bioversity International 5 purple-brown 3 fat (w/y equal to or more than 0.55) Rhiannon Crichton Bioversity International PltDnsty_Comp_PlntHa equal to or more than 31 cm 3 other 5 5 rounded dark brown 2 pointed 1 3 curved (sharp curve) NARO, Uganda MatSurfHomColV_Meas_0to10 Elizabeth Kakhasa Matooke surface homogeneity of colour measurement scale 1-10 Rhiannon Crichton ShtHvstTime_Comp_d Bioversity International Bioversity Noel Madalla PA_EstMale_Txt extensive pigmentation 4 CIRAD / UNA, Côte d’Ivoire Hermann Antonin KOUASSI Boiled plantain Moistness scale 0-10 BoiPlantnMois_Meas_0to10 rwcPseudostem_Comp_unitless rwcPseudostem ps_rwc KU Leuven Sebastien Carpentier 1 brown Rhiannon Crichton PltFstLfSptd_Ct_leaf Bioversity International FstExtDiSmptFW_Date_ymd Rhiannon Crichton Bioversity International 4 neutral/male flowers and presence of withered bracts on the entire stalk 3 hanging at a 45 degree angle temp8 Rhiannon Crichton Bioversity International blunt-tipped (plateau at tip) 3 FrtGrth_M_mm Rhiannon Crichton Bioversity International Noel Madalla Selection criteria for genotype that was ranked third highest by male respondents during preference scoring exercise Bioversity 2 First streak stage: specks elongate, becoming slightly wider to form narrow reddish-brown streaks. IITA-Bioversity International BlkLfStrkSvrt_Est_MedLawCpt IITA-Bioversity International Amos Alakonya-Rhiannon Crichton FstYngLfsptd_Est_dmy other 8 BrctClExtFc_Est_1to16 Rachel Chase Bioversity International straight (or slightly curved) 1 Bioversity International temp31 Rhiannon Crichton Bioversity International Rhiannon Crichton PltDamDesc_Est_txt Bioversity Noel Madalla PS_CompFemale_PrefScale purple 5 Stage 1 appears as a small depigmentation spot whose whitish or yellow colour resembles stage 1 of Yellow Sigatoka disease. These symptoms are not visible in transmitted light and can be observed only on the underside of the leaf. 1 IITA AvgWvlLvBdLgt_M_µM Tendo Ssali yellow 2 Tendo Ssali IITA DmgIdxInn_M_% Rhiannon Crichton MltHandWt_M_kgPlnt Bioversity International green 1 massDryPseudostem ps_dry pseudostemDryMass_Meas_g KU Leuven Sebastien Carpentier less than or equal to 12 1 0 no intensity 3 asymmetrical ** 2 other 8 temp17 Bioversity International Rhiannon Crichton 1 without flower relicts massDryPlant KU Leuven Sebastien Carpentier plantDryMass_Meas_g plant_dry 1-2 egg masses 1 heterogeneous 0 AnCrpCyclPrp_Comp_CrpCclY Rhiannon Crichton Bioversity International not clasping 2 1 absent 2 sparse blotching (<20%) 50-75 % of roots galled 4 Rhiannon Crichton temp41 Bioversity International white 1 Bioversity International Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd6 1 pointed DeadRtNum2_Ct_Rt Bioversity International/IITA Inge Van den Bergh/Danny Coyne Bioversity International/IITA temp48 Inge Van den Bergh/Danny Coyne Rhiannon Crichton temp10 Bioversity International Stellenbosch University temp37 Altus Viljoen IITA-BTI Guillaume Bauchet AvgFrtIntLng_M_cm banana bunchy top (BBTD) 4 other 4 Rhiannon Crichton Bioversity International CigLfUnrBrnStgB_Date_ymd Second-spot stage: the dark brown or black central area of the spot becomes slightly depressed and the water-soaked border becomes more pronounced due to darkening. At this stage, a slight yellowing of the leaf tissue immediately surrounding the water-soaked border may occur. 5 Rhiannon Crichton AvgFrtGrth_M_mm Bioversity International 3 intermediate DamEst_Date_dmy Rhiannon Crichton Bioversity International orange-brown (mahogany, like in Pisang Mas) 1 4 horizontal 0 very light yellow Bioversity International Rhiannon Crichton CigLfUnrBrnStgB_Date_dmy Rachel Chase Bioversity International PMargWing_Est_1or2 4 First-spot stage: the streak broadens and becomes more or less fusiform and elliptical in outline and similar to the first-spot stage of Sigatoka (Leach 1946). The transition from streak to spot is further characterised by the development of a light brown, water-soaked border around the spot. This water-soaked effect is especially clear in the early morning, when dew is still present on the leaf, or after rain. IITA Tendo Ssali AvgWvlLvHdCps_M_µM 10 other Bioversity PS_CompResp_PrefScale Noel Madalla acronym Inge Van den Bergh Bioversity International LfChgPres_Est_0or1 Tendo Ssali WvlAdltBdWght_M_mg IITA high intensity 10 Bioversity International BlkLfStrkSvrtLfNb_Ct_grd5 Rhiannon Crichton cream 1 Bioversity Noel Madalla Selection criteria for genotype that was ranked lowest by female respondents during preference scoring exercise 2 not parthenocarpic Rhiannon Crichton MltFrtWt_M_gFruit Bioversity International 2 wide with erect margins 10 firm 6 medium green mole rats 8 NARO, Uganda Matooke astringency measurement scale 0-10 MatAstr_Meas_0to10 Elizabeth Kakhasa Stage 3 differs from the previous one by its dimensions. The stripe becomes longer, is enlarged and in certain conditions (weak inoculum and unfavourable climatic conditions) can reach 2 or 3 cm. 3 9 other Bioversity International Rachel Chase FrtCvShp_Est_1to6 Rhiannon Crichton Bioversity International PltNtHvstd_Ct_Plnt 2 partially fused (up to 50 % of the length of the pedicel) margins curved inward 3 5 light green grey 4 Bioversity International DateHarv_Date_dmy Rhiannon Crichton Rhiannon Crichton BlkLfStrkLfFrStg1_Date_ymd Bioversity International smooth 10 5 obtuse and split 3 brown-black extensive pigmentation (>50%) 4 KU Leuven Sebastien Carpentier leaf_fresh leafFreshMass_Meas_g massFreshLeaf Selection criteria for genotype that was ranked second highest by female respondents during preference scoring exercise Bioversity Noel Madalla 3 male flowers/bracts above the male bud (rest of stalk is bare) truncate ( 2 Second-spot stage: the dark brown or black centre of each lesion becomes slightly depressed and water soaked border becomes more pronounced. 5 Stellenbosch University Altus Viljoen temp38 4 curved in S shape (double curvature) high intensity 10 6 other 5 erect banana Xanthomonas wilt (BXW) 3 2 good 0 absent red-purple 4 Initial speck stage: symptoms are first visible to the naked eye as faint, minute (less than 0.25 mm diam), reddish brown specks on the lower surface of the leaf. Specks are often most abundant near the margin of the left side of the leaf, particularly towards the tip. Where leaf spot is severe, specks have been observed on the second leaf of plants that have not yet produced a bunch. Elsewhere they usually appear on the third, fourth, or older leaves. 1 FrtApxFlwRlt_Est_1to3 Rachel Chase Bioversity International Bioversity International PtYd_Comp_TnHaY Rhiannon Crichton 5 banana streak virus (BSV) 2 slightly angled 1 adult male Rachel Chase Bioversity International FrtLngth_M_cm 1 add** 1 less than or equal to 2 m short (y less than or equal to 20 cm) 1 1 dwarf 5 without pigmentation 1 white ovoid 4 Bioversity International OffTypEst_Date_dmy Rhiannon Crichton 6-15% of lamina with symptoms 3 1 open with margins spreading base of the style prominent 3 31-100 egg masses 4 cream 2 Tendo Ssali WvlAdltPrTrp_Ct_weevil IITA Bioversity International PltEst_Date_dmy Rhiannon Crichton 0 no egg masses orange red 7 dark green 8 3 Second-streak stage: the streaks may elongate slightly, but the most notable change is that in colour from reddish brown to dark brown or almost black, sometimes with a purplish tinge. The streak is now clearly visible on the upper surface of the leaf. The distribution of streaks at this stage of development varies in the same way as mentioned above. When streaks are very numerous, and more or less evenly distributed, the entire leaf blackens. 7 green FrtLngth_M_1to5 Rachel Chase Bioversity International dark green 8 Selection criteria for genotype that was ranked lowest by male respondents during preference scoring exercise Noel Madalla Bioversity 4 26-30 cm red-purple 2 absent 0 2 not dwarf < 1/3 discoloured 3 4 obtuse and split black-purple 4 neutral flowers on one to few hands only near the bunch (rest of stalk is bare) 2 PA_EstFemale_Txt Bioversity Noel Madalla parthenocarpic 1 4 green yellow only base of the style persists 4 Second-streak stage: streaks change colour from reddish brown to dark brown or black, sometimes with a purplish tinge, clearly visible at the upper surface of the leaf. 3 3 obtuse intermediate 3 2 11-20 mm Banana ontology Plantain ontology 6 purple brown 10 homogeneous 3 red 1 truncated 2 one yellow finger 7 blue 3 totally fused (more than 50 % of the length of the pedicel) sticky 10 straight 1 3 21-25 cm discolouration of between one-third and two-thirds of vascular tissue 4 medium intensity 5 equal to or more than 3 m 3 9 orange-red 1 revolute (rolling) 1 less than or equal to 15 cm 1 present purple to blue 3 Tendo Ssali IITA AvgWvlLvBdWght_M_mg margins spreading 1 8 pink-purple straight with erect margins 3 egg 4 7 black (anthers aborted) 0 low intensity 3 black-purple 5 curved in slight S shape (double curvature) firm 5 erect 5 5 brown/rusty brown 5 Stage 5 is when the elliptical spot is totally black and has spread to the underside of the limb. It is surrounded by a yellow halo with the centre beginning to flatten out. 1 no visual leaf symptoms 5 light green 2 dont know 2 < 25 % of roots galled 2.1-2.9 m 2 less than or equal to 10 mm 1 low shouldered (x/y more than or equal to 0.30) 3 dislike 2 corm completely clean, no vascular discolouration 1 1 bad 5 plant dead, with brown leaves hanging down the pseudostem 16-33% of lamina with symptoms 4 7 green 2 medium (20 cm < y < 30 cm) 2 few internal spots 1 present 2 First streak stage: the initial speck elongates, becoming slightly wider, to form a characteristic narrow, reddish brown streak up to 20 mm long and 2.0 mm wide, with the long axis parallel to the leaf venation. At this stage, streaks are more clearly visible on the lower surface of the leaf than on the upper one. The distribution of streaks is variable. Sometimes they are most numerous near the edge of the left side of the leaf. At other times they are equally numerous on both sides of the leaf, and more or less evenly distributed. Frequently they are densely aggregated in a band several cm wide on one side or both sides of the mid-rib, becoming less numerous towards the edge of the leaf. Streaks may be so numerous that every cm2 of leaf surface bears one or more. There is considerable variation in the length of individual streaks on a given leaf, and they frequently overlap to form larger, compound streaks. 1 sparse blotching 0 Dry entity Visually observe the remains, or not, of flower relicts at the finger apex, and choose the option that is most similar, from the 4 photos of descriptor 6.7.7 in the reference materials. Remains of flower relicts at fruit apex - estimation Degree of fusion of the pedicels before they join the rachis Fusion Fusion of pedicels Colour Fruit smashed pulp colour The color of the smashed pulp of a mature fruit Ratio of belowground (root) to aboveground (shoot) dry mass Ratio - computation tallsuckererfunctleaves The number of leaves on the tallest sucker that have 50 % or more of the leaf surface area as green, healthy, photosynthetic tissue. Number of functional leaves Tallest sucker number of functional leaves Moldability in hand method Try to make a ball (agglomerate) of the sample and evaluate how easy it is to deform or break the sample Rank of youngest leaf spotted Youngest leaf spotted The rank (order) of the first fully unfurled leaf with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 leaf spot light-coloued dry centres, caused by black leaf streak disease. YLS The overall estimation of sucker quality on a mat Overall suckering quality suckering Sucker quality aroma scale 0-10 Average weight The average weight of the fingers. Average finger weight avefingerweight Water content - computation (Leaf Fresh Weight - Leaf Dry Weight) / Leaf Fresh Weight The length of a leaf Single leaf length singleLeafLength Length Fruit unsmashed pulp texture The texture of the unsmashed pulp of a mature fruit Texture crop cycle duration/year Sweetness scale 0-10 Presence of root knot nematodes Nematode root knot galling presence The presence of root knot nematodes, observed externally as galls on the roots. Calculate as: the time elapsed between the Date of planting to the Date of harvest. E.g. 24/12/2014 - 24/01/2014 = 334 days. Plant crop cycle - computation Rank of oldest standing leaf - counting Record the rank (order) of the oldest standing leaf (with an erect petiole), counting the rank by starting with the youngest completely unrolled leaf as 1 and moving downwards, associating the data with the Date of data collection. Growth - computation Fresh weight difference between timepoints (refer to experimental metadata) Percentage of plants with yellowing leaves caused by Fusarium wilt - computation Calculate as: the sum of the number of values for Presence of yellowing leaves caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 78 / 300 * 100 = 26%. Harvest density The number of plants that are harvested, in a defined area. Combined density of plants harvested harvdens Dry mass accumulation over time growthDry Growth Dry plant growth multiplication factor Mealiness scale 0-10 Ratio - computation Ratio of root dry mass versus plant dry mass Ratio of pseudostem dry mass versus plant dry mass Ratio - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 1. Number of leaves with black leaf streak disease severity grade 1 - computation Number of fingers in bunch - counting Count how many fingers are in the bunch. dd/mm/yyyy Average hand weight - all - computation Calculate as: the Total finger weight, divided by the Number of hands in bunch, e.g. 46.8 / 16 = 2.9. Number of nematodes per unit of fresh weight Root nematode density The number of nematode individuals per unit of fresh root weight. Overall bunch quality The overall estimation of the bunch quality on a mat goodbunch Quality Weevil larvae head capsule - measurement Measurement under microscope Weekly leaf emission rate - computation Calculate as: the Rank of previously marked leaf at one point in time, minus 1 (equivalent to the Rank of marked youngest leaf), divided by the time elapsed between the two Date of data collection events when the marked leaf was recorded as 1) the Rank of marked youngest leaf and 2) the Rank of previously marked leaf, e.g. (2 - 1) / 1 week = LER of 1 leaves/week during a particular time period. Calculate as: the sum of the the Finger weight measurements, divided by the number of those measurements, e.g. (150 + 146 + 147 + 152 + 140 + 146) / 6 = 147. Average finger weight - computation The thickness of the peel of a finger. Thickness of the peel peelthickness Finger peel thickness The circumference of the pseudostem of the plant at 100 cm from the collar. Circumference 1 m above the ground Plant circumference 100 cm from the collar plantcircumf100cm Fresh weight - measurement Weighing Margin behaviour on petiole canal of third leaf Margin behaviour of the petiole canal of the third leaf before bunch. Behaviour m2 Measure the lateral diameter of a finger from the left to the right side (not from the ventral to the dorsal side), at the widest point, using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger lateral diameter - measurement Presence Male rachis type Behaviour of male rachis - truncated or present. Truncated means there is no bract scar below the last hand. Present means there is a degenerated or persistent male bud Plant spacing - computation Calculate as: multiply the distance of the spacing between the plants, both latitudinally and longitudinally, e.g. 2 m x 3 m = 6 m2. Visual estimation of a fruit fill, (or parthenocarpic) for Early Evaluation Trial stage only. Fruit parthenocarpy - estimation Visual assessment. Presence of splitting pseudostem base caused by Fusarium wilt - estimation No. per 100ml cm? fingerintlength The length of a finger measured along the internal (ventral) arc, excluding the pedicel and the fruit tip. Internal length Finger internal length Presence of changes in new leaves caused by Fusarium wilt CNL Presence/absence of changes The presence/absence of changes in new leaves - irregular pale margins, narrowing of lamina, burning plus ripping of lamina, lamina becoming more erect, caused by Fusarium wilt. Average hand weight avehandweight The average weight of the hands. Average weight The lifecycle stage(s) of the nematode species found in the banana plant root sample. Nematode lifecycle stage Lifecycle stages present % The amount of damage to the outer corm (cortex) lower cross-section, assessed by percentage of tunnels caused by weevils. Damage percent X-lower-outer Percentage damage to lower cross-section outer corm caused by weevils Lobe colour of tip of compound tepal - estimation The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the colour of the lobe at the tip of the tepal. Use colour chart B and observe out of direct sunlight. mL rankprevleaf The current rank (position) of the leaf that was previously marked as the youngest leaf at the time of the last data collection event. Rank Rank of previously marked leaf Calculate as: the Plant density multiplied by the Non-harvest proportion, e.g. 1,667 * 0.08 = 133. Non-harvest density - computation Fresh weight A root weight trait which is the fresh weight of a root rootFreshMass Root fresh weight open answer Pseudostem fresh weight pseudostemFreshMass Fresh weight A pseudostem weight trait which is the fresh weight of a pseudostem Pest and/or disease assessment and/or sample collection description pestdiseasesampledescription Pest/disease assessment/sample collection description A description of the pest and/or disease assessment and/or sample collection being done at the time of data collection. unitless Number of Meloidogyne spp. per soil sample Population density Number of Meloidogyne spp. in soil The dry weight of a leaf leafDryMass Leaf dry weight Dry weight Smoothness in mouth Matooke smoothness geometrical textural attribute relating to lack of presence of particles in a product MatSmoothM Matooke Description of how off-type Off-type description A description of what made the plant recogniseable as an off-type. describeofftype Weighing Fresh weight - measurement Number of banana weevil larvae measured Number of banana weevil larvae measured How many banana weevil larvae used to obtain a measurement. Fruit shape of curve - estimation Visually observe the shape of the curve of the fruit and choose the option that is most similar, from the 5 photos of descriptor 6.7.4. in the reference material. Observe the inner fruit in the middle of the mid-hand of the bunch. In case of an asymmetric bunch that has straight and curved fruits, please indicate it in the note section and score only the fruit on the upper side of the bunch. Combined percentage with internal symptoms of Fusarium wilt Percentage of plants with internal disease symptoms caused by Fusarium wilt The percentage of plants with any or multiple of the internal disease symptoms - discolouration in the corm, and/or discolouration in the pseudostem, caused by Fusarium wilt. The date damage to the plant is first observed. Date of damage datedamage Date of damage The number of plants that are dead, in a defined area. Combined density of plants that are dead deathdens Death density Date of flowering dateflower Date of flowering The date the last bracts fall from the most distal hand (closest to the male bud) to display the female flowers. Weighing Fresh weight - measurement Presence of changes in new leaves caused by Fusarium wilt - estimation Visual assessment. Non-harvest density nonharvdens Combined density of plants not harvested The number of plants that are alive but not harvested (e.g. due to slow or poor development), in a defined area. Boiled plantain Sweetness Boiled plantain Sweetness Elemental flavor caused by dilute aqueous solutions of various substances such as sucrose or aspartame BoiPlantnSwt Multiple finger weight - measurement Weigh multiple fingers together, using scales. Associate the data with the Hank rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. % The pulp aroma of the smashed pulp of a mature fruit Fruit smashed pulp aroma/smell Aromas Time elapsed between planting and the appearance of the first youngest leaf spotted. Time from planting to first youngest leaf spotted Time elapsed from planting to first youngest leaf spotted Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant, do not include roots from adjacent plants. Carefully wash the soil from the roots with tap water. Cut the roots into 10 cm long pieces and dry with paper tissue. Take a subsample of 15 g, add 100 ml distilled water and store the roots at 4 degrees Celsius. Put the roots in 100 ml of distilled water in a kitchen blender. Macerate the roots 3 times for 10 secs (separated by 5 sec intervals). Pour the macerated suspension through 250, 106 and 40 micro-metre sieves and rinse the sieves with tap water. Using distilled water, collect in a beaker the nematodes from the 40 micro-metre sieve. Using distilled water, dilute to 200 ml the nematode suspension in a graduated cylinder. Blow air through the nematode suspension with a pipette (to homogenise the suspension). Take a subsample of 6 ml (counting dish) or 2 ml (counting slide). Count the nematodes in the counting dish (stereo microscope) or in the counting slide (light microscope). Calculate the final nematode population per unit of fresh root weight. Root nematode density - computation Finger weight - measurement Weigh a finger, using scales. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Percentage of plants with yellowing leaves caused by Fusarium wilt Combined percentage with yellowing leaves Percentage of plants with yellowing leaves, caused by Fusarium wilt. Water content - computation (Pseudostem Fresh Weight - Pseudostem Dry Weight) / Pseudostem Fresh Weight Matooke Pumpkin-like aroma Matooke aroma pumpkin Aroma of pumpkin. Possibly, precise if the taste is like boiled pumpkin or fried... MatAroPumpA Stickiness in hand method Put a part of the sample between thumb and index fingers and using tapping motions, evaluate the amount of product adhering on them Boiled plantain Energy or number of chews necessary to chew the banana to make it ready to be swallowed BoiPlantnChew Chewiness Boiled plantain Chewiness Number of nematodes (Radopholus similis ) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Number of fingers measured - counting Count how many fingers are used to obtain a measurement. The weight of a banana weevil adult weevil body. Banana weevil adult body weight Body weight harvprop Harvest proportion The proportion of plants that are harvested, from the number of plants planted. Combined proportion of plants harvested Percentage of plants with changes in new leaves caused by Fusarium wilt - computation Calculate as: the sum of the number of values for Presence of changes in new leaves caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 67 / 300 * 100 = 22%. Visually observe and record the amount of the surface area of a standing leaf that is affected by black leaf streak disease. Black leaf streak disease severity - estimation 1 to 5 acceptability score Visual appearance - estimation Subjective visual assessment of smashed and unsmashed fruit pulp Main colour of the pseudostem under the outermost sheath. Main underlying colour of pseudostem Main underlying colour Rating of internal symptoms of Fusarium wilt in the greenhouse - estimation Visual assessment. Remains of flower relicts at fruit apex Remains of flower relicts at apex Percentage of flower relicts at fruit apex Height Plant height to neck of peduncle plantheightneck The height of the pseudostem of the plant from the collar to the curved neck of the peduncle. Stunting expressed as height growth per new formed leaf pseudostemHeightIncreasePerFormedLeaf Growth Pseudostem height increase per leaf formed Total finger weight - measurement Cut the hands off from the rachis and weigh all the hands together, using scales. Distance of internal discolouration in pseudostem of plant caused by Fusarium wilt The distance of discolouration in the pseudostem of the plant, caused by Fusarium wilt. Distance of discolouration Number of Pratylenchus spp. per fresh root weight Population density Number of Pratylenchus spp. in roots Count how many hands are in the bunch. Number of hands in bunch - counting Damage to upper outer corm weevils - measurement Cut a transverse cross section of the corm at the collar (upper cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Number of adult weevils measured How many banana weevil adults used to obtain a measurement. Number of banana weevil adults measured Average number of banana weevil larvae The average of how many banana weevil larvae caught per trap. Average number of banana weevil larvae per trap Ratoon crop cycle The time elapsed from the harvest of the bunch of the plant from the preceding crop cycle to the harvest of the bunch of the plant from the current crop cycle. Ratoon crop cycle harvest2harvest blotch pigmentation scale (1 to 5) Calculate as: the sum of the number of plants with any or multiple of the internal disease symptoms caused by Fusarium wilt - discolouration in the corm, and/or discolouration in the pseudostem - divided by the Number of plants planted * 100, e.g. 108 / 300 * 100 = 36%. Percentage of plants with internal disease symptoms caused by Fusarium wilt - computation How many banana weevil larvae caught per trap. Number of banana weevil larvae per trap Number of banana weevil larvae Average finger peel thickness - computation Calculate as: the sum of the Finger peel thickness measurements, divided by the number of those measurements, e.g. (2 + 2 + 2 + 3 + 2 + 3 + 3) / 6 = 2. Presence of yellowing leaves caused by Fusarium wilt The presence/absence of yellowing leaves, caused by Fusarium wilt. YL Presence/absence of yellowing Black leaf streak disease evolution time - computation Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 and the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1, e.g. 24/06/2014 - 24/04/2014 = 61 days. Identify the nematode species present in the banana plant root sample (Radopholus similis, Pratylenchus coffeae, Pratylenchus goodeyi, Helicotylenchus multicinctus, Meloidogyne incognita, ?) Nematode species - estimation Petiole margin colour - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Use colour chart A and observe out of direct sunlight. Record the colour of the margin (general colour is below the rim). [x Observe at flowering time.] finger Count how many fingers are in multiple hands. Associate the data with the Hand rank and the Number of hands measured. The recommendation is to collect this data from the second and second-most distal hands. Multiple number of fingers in hand - counting Measurement under microscope Adult weevil head capsule - measurement Taste - estimation Subjective taste assessment of smashed and unsmashed fruit pulp The average weight of a banana weevil adult body. Average body weight Banana weevil adult average body weight hand The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the colour of the backside middle of tepal. Use colour chart B and observe out of direct sunlight. Compound tepal main colour - estimation Apex shape Bract apex shape Shape of the first external unlifted bract of the male bud Date of shooting Date of shooting dateshoot The date the inflorescence emerges from the pseudostem and is still in an erect position. Genotype selection traits Description of traits considered important in selection of best genotype by respondents Selection criteria for genotype ranked highest by respondents Growth - computation Pseudostem height difference between timepoints (refer to experimental metadata) per number of new leaves formed between timepoints (refer to experimental metadata) Count how many fingers are in a hand. Associate the data with the Hand rank. The recommendation is to collect this data from the second and second-most distal hands. Number of fingers in hand - counting Fruit pedicel length - exact value - measurement Measure from the scar on the rachis until the beginning of the fruit. Record on the inner fruit in the middle of the mid-hand of the bunch. Tip: use string to measure or trace outline of fruit on paper. Record the exact value. Calculate as: the time elapsed between the Date of planting and the Date of death, e.g. 24/05/2014 - 24/01/2014 = 120 days. Time from planting to death - computation bunch shape scale (1 to 5) kg/plant/y Calculate the ratio w/y, i.e. the broadest width of the male bud to the total length of the male bud. Do not measure the dimensions along the bud but rather on a projection/outline of the bud (e.g. trace the outline of the bud on paper) and use ruler or measuring tape. Male bud shape - computation Black leaf streak disease severity index - computation Calculate as: ((((Number of leaves with black leaf streak disease severity grade 0, multiplied by 0), plus (Number of leaves with black leaf streak disease severity grade 1, multiplied by 1), plus (Number of leaves with black leaf streak disease severity grade 2, multiplied by 2), plus (Number of leaves with black leaf streak disease severity grade 3, multiplied by 3), plus (Number of leaves with black leaf streak disease severity grade 4, multiplied by 4), plus (Number of leaves with black leaf streak disease severity grade 5, multiplied by 5), plus (Number of leaves with black leaf streak disease severity grade 6, multiplied by 6)), divided by the number of grades in the scale minus 1 (i.e. 7-1)), divided by the Number of standing leaves), multiplied by 100. For example, ((((3 * 0) + (1 * 1) + (1 * 2) + (3 * 3) + (5 * 4) + (0 * 5) + (0 * 6)) / 6) / 13) * 100 = ((32 / 6) / 13) * 100 = 41. dateendtimedatacoll Date and time of end of data collection The date and time of the end of data collection on a plant. End date and time of data collection Measure the circumference of the pseudostem of the plant at 75 cm from the collar, or from the pseudostem base at the ground if the collar is not visible, using a tape measure. Plant circumference 75 cm - measurement Pseudostem height increase over time Height increase of pseudostem pseudostemHeightIncrease Growth dwarfism Transpiration efficiency The growth (weight increase) per volume transpired water Efficiency transpirationEfficiency Rachis weight Weight The weight of the rachis. rachisweight Calculate as: the sum of the Hand weight measurements from all the hands in the bunch (number equivalent to Number of hands in bunch), e.g. 2.4 + 3.6 + 3.2 + 4.1 + 4.0 + 3.9 + 3.6 + 3.3 + 3.3 + 2.9 + 2.9 + 2.8 = 40.0. Total finger weight - hands - computation The circumference of a finger. fingercircumf Finger circumference Circumference Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked lowest in preference ranking exercise Focus group discussion for lowest ranked traits with female respondents Fruit pulp colour Pulp colour fruitpulpcolour The colour of the fruit pulp. Number of nematodes (Hoplolaimus spp.) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample plant Count how many functional leaves (leaves that have 50% or more of their surface as green, healthy, photosynthetic tissue) are on the tallest sucker. Tallest sucker number of functional leaves - counting Length of the fruit pedicel from rachis to the beginning of the pulp Length Fruit pedicel length numfingers2ndhand Number of fingers The number of fingers in the second hand. Number of fingers in 2nd hand Matooke Color of the surface of the sample from light yellow to bright yellow MatSurfYellV Matooke surface yellowness intensity Surface yellowness intensity Calculate as: the time elapsed between the Date of planting or the Date of harvest of the bunch from the plant of the previous crop cycle and the Date of first external disease symptoms caused by Fusarium wilt. Time from start of crop cycle to first external disease symptoms caused by Fusarium wilt - computation Put a part of the sample in the mouth, chew and evaluate the quantity of water within the sample. Moisture in mouth method Calculate as: the Number of plants not harvested, divided by the Number of plants planted, e.g. 2 / 24 = 0.08. Non-harvest proportion - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 5. Number of leaves with black leaf streak disease severity grade 5 - computation rankyoungleaf The rank (position) of the youngest completely unrolled leaf. Rank Rank of marked youngest leaf Record a description of the state of the plant. Plant state description - estimation Record the date of event. Date of weevil damage to corm assessment - estimation Description of traits considered important in selection of third best genotype by respondents Selection criteria for genotype ranked third highest by respondents Genotype selection traits Ex: g/100 mL juice???? Plant density plantdens The number of plants that are planted, in a defined area. Combined density of plants planted Number of hands on whole bunch - counting On a bunch with mostly hands of > 10 fingers, a possible ultimate hand with 1-5 (rather smaller) fingers should not be counted. kg MatHardT Hardness in hand mechanical textural attribute relating to the force required to achieve a given deformation, penetration, or breakage of a product. Matooke Matooke hardness Root knot nematode galling Damage to lower inner corm weevils - measurement Cut a transverse cross section of the corm at 10 cm below the collar (lower cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. PS Calculation of preference score by respondents Overall plant appearance Preference score Lobe colour at tip of the tepal Lobe colour of tip of compound tepal Main colour of the tip of the compound tepal (lobe) The third leaf (Leaf III) is counted from the last leaf produced before [x bunch emergence] [shooting]. Cut the petiole halfway between the pseudostem and the leaf blade and examine the cross section. [x Observe at flowering time]. Margin behaviour on petiole canal of third leaf - estimation pulp colour LER Monthly leaf emission rate A rate that expresses the number of leaves that have emerged from the pseudostem in a month. Monthly leaf emission rate Number of fingers in bunch - computation Calculate as: the sum of the Number of fingers in hand measurements from all the hands in the bunch (number equivalent to Number of hands in bunch), e.g. 16 + 17 + 19 + 18 + 19 + 18 + 17 + 17 + 16 + 14 + 14 + 13 = 198. Matooke homogeneity of surface colour Uniformity of color of the surface of the sample MatSurfHomColV Surface color homogeneity Matooke Calculate as: the Number of dead roots divided by the Number of all roots, multiplied by 100, e.g. 8 / 20 * 100 = 40 % dead roots. Percentage of dead roots - computation Extent of internal discolouration in corm of plant caused by Fusarium wilt - estimation The extent to which vascular discolouration extends up the pseudostem should be determined by making cross-section cuts, from the base of the pseudostem upwards, and examining the internal tissues following each cut. The point at which discolouration is no longer visible should be noted and the distance from this point to the pseudostem base recorded. Visual estimation of dwarfism, for Early Evaluation Trial stage only. Dwarfism - estimation Stickiness in hand mechanical textural attribute relating to the force required to remove material that sticks to the mouth Matooke Matooke stickiness MatStickT color homogeneity scale 0-10 cm The proportion of plants that are alive but not harvested (e.g. due to slow or poor development), from the number of plants planted. Combined proportion of plants not harvested Non-harvest proportion nonharvprop maiden sucker Presence of yellowing leaves caused by Fusarium wilt - estimation Visual assessment. Plant water loss waterLossPlant Volume The amount of water loss through the plant over time Fusarium wilt internal symptoms scale MatSweet basic taste produced by dilute aqueous solutions of natural or artificial substances such as sucrose Matooke sweetness Sweetness Matooke weevil Grassy-like Aroma method Put a part of the product and by retro-olfaction evaluate the presence and the intensity of grassy-like aroma bud shape scale (1 to 3) The length of a finger measured along the external (dorsal) arc, excluding the pedicel and the fruit tip. External length fingerextlength Finger external length bract apex scale (1 to 5) Rachis weight - computation Calculate as: Bunch weight minus the sum of the Hand weight measurements coming from the count of number of hands (equivalent to Number of hands in bunch), e.g. 50 - (2.4 + 3.6 + 3.2 + 4.1 + 4.0 + 3.9 + 3.6 + 3.3 + 3.3 + 2.9 + 2.9 + 2.8) = 10.0. Percentage of plants with petiole collapse caused by Fusarium wilt Percentage of plants with petiole collapse, caused by Fusarium wilt. Combined percentage with petiole collapse The date of the appearance on the plant of any of the external disease symptoms - yellowing leaves, splitting pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Date of appearance of external symptoms caused by Fusairum wilt Date of first external disease symptoms caused by Fusarium wilt Record the date of event. Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 - estimation Number of weevil larvae - counting Visual counting method Boiled plantain Firmness Firmness Force required to obtain deformation, penetration or rupture of the banana Boiled plantain BoiPlantnFirm Blotches area - estimation Record the relative surface area coverage by blotches. [x Look at several plants if possible to get an overall idea. Observe at flowering time]. Record the rank of the previously marked leaf (the leaf that was marked in the Rank of marked youngest leaf during the last data collection), counting the leaf rank by starting with the newest completely unrolled leaf as 1 and moving downwards. Associate the data with the Date of data collection. Rank of previously marked leaf - counting The average weight of a banana weevil larvae body. Banana weevil larvae average body weight Average body weight Calculate as: the time elapsed between the Date of flowering to the Date of harvest, e.g. 24/12/2014 - 24/10/2014 = 61 days. Time from flowering to harvest - computation Measure from the scar on the rachis until the beginning of the fruit. Record on the inner fruit in the middle of the mid-hand of the bunch. Tip: use string to measure or trace outline of fruit on paper. Record the range. Fruit pedical length - range - measurement Put a piece of banana in your mouth, chew it and swirl it around your tongue to detect the sweet flavor Taste method Sweetness mg Focus group discussion for top traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked highest in preference ranking exercise plants dead/plants planted Number of Rotylenchulus spp.in soil Number of Rotylenchulus spp.per soil sample Population density Fruit length - exact value - measurement Measure the length of the internal arc of a fruit, without pedicel. Record on the inner fruit in the middle of the mid-hand of the bunch. If there is an even number of hands, there will be two middle hands so use the upper hand that developed first. Record the exact value. Number of leaves with a black leaf streak disease severity grade of 4 Number of leaves with black leaf streak disease severity grade 4 The number of leaves on a plant with a black leaf streak disease severity grade of 4. Stickiness scale 0-10 day Average finger lateral diameter avefingerlatdiam Average lateral diameter The average lateral (side to side) diameter of a finger. Leaf area - measurement Top view image of loose leaves Cut a hand from the rachis and weigh the hand, using scales. Associate the data with the Hand rank. Hand weight - measurement The number of leaves on the plant that have 50 % or more of the leaf surface area as green, healthy, photosynthetic tissue. Number of functional leaves Number of functional leaves functleaves Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards. [x Observe at flowering time.] Winged petiole margin - estimation Pseudostem colour - estimation Detach the outermost sheath from the pseudostem (the sheath should not be too dry). Record the overall impression of colour of the exposed surface of the underlying pseudostem. Note that this main colour should cover more than 75% of the underlying pseudostem surface. Use colour chart A and observe out of direct sunlight. plant2harvest The time elapsed from planting to the harvest of the bunch. Plant crop cycle Time from planting to harvest Average finger circumference - computation Calculate as: the sum of the Finger circumference measurements, divided by the number of those measurements, e.g. (7.0 + 8.0 + 9.0 + 10.0 + 7.0 + 9.5) / 6 = 8.4. singleLeafWidth Single leaf width Width The width of a leaf The time elapsed from planting to shooting (when the inflorescence emerges from the pseudostem and is still in an erect position). Time from planting to shooting Time from planting to shooting plant2shoot Number of nematodes (Rotylenchulus spp.) per unit fresh root weight - method Count from 100 ml of soil sample Percentage of plants with external disease symptoms caused by Fusarium wilt The percentage of plants with any or multiple of the external disease symptoms - yellowing leaves, splitting of pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Combined percentage with external symptoms of Fusarium wilt Nematode egg-laying females extent Extent of root knot nematodes The extent of egg-laying female root knot nematodes, observed internally as pit-like structures on the roots. Moldability in hand mechanical textural attribute relating to the degree to which a substance can be deformed before it breaks Matooke moldability MatMolT Matooke Finger external length - measurement Measure the length of a finger that is at the ready to eat stage, along the external (dorsal) arc, excluding the pedicel and the fruit tip, using a tape measure. Associate the data with the Hand rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. BD colour chart A - 2016 Presence Colour line along edge of petiole margin Presence or absence of colour line along edge of petiole margin Measure for the biomass allocation: dry mass of leaves versus whole plant dry mass ratio leafToPlantMassRatio_dry Leaf to whole plant mass ratio Ratio bunch position scale(1 to 5) Anther colour - estimation The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the anther colour on the face opposite to the dehiscence split of the anther. Use colour chart B and observe out of direct sunlight. Number of plants planted - computation Calculate as: the sum of the number of values for Date of planting. Measurement under microscope Weevil larvae body length - measurement Behaviour of the petiole margins - winged or not winged. the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Petiole margins winged Wing presence smoothness scale 0-10 Time from planting to first youngest spotted leaf - computation Calculate as: the time elapsed between the Date of planting and the Date of first youngest leaf spotted, e.g. 24/06/2014 - 24/01/2014 = 151 days. Petiole margin clasping - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Observation should be made at shooting on the neck, where the petiole and pseudostem meet. Margin is the part of the petiole that can be bent outwards/inwards. [x Observe at flowering time.] Number of spotted leaves The number of leaves with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres, caused by black leaf streak disease. Number of spotted leaves Put a part of the product and by retro-olfaction evaluate the presence and the intensity of matoke-like aroma Matooke-like Aroma method Manual height measurement Length - measurement Ratio rootToPlantMassRatio_dry Root to whole plant mass ratio Measure for the biomass allocation: dry mass of roots versus whole plant dry mass ratio µM g / mL Number of nematodes (Helicotylenchus multicinctus) per unit fresh root weight - method Count from 100 ml of soil sample The overall estimation fruit pendulance on a bunch Overall fruit pendulance quality Finger pendulance quality pendulantfruit Stunting expressed as number of leaves per height growth leavesFormedPerPseudostemGrowth Formation Leaves formed per pseudostem height increase Fusion of pedicels - estimation Visually observe the fusion of pedicels and choose the option that is most similar, from the 3 photos of descriptor 6.7.11. in the reference materials. Observe before they join the rachis at the bract scar. Look upwards from the bottom of the bunch. Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Pratylenchus spp.) per unit fresh root weight -method Calculate as: the Number of functional roots divided by the Number of all roots, multiplied by 100, e.g. 12 / 20 * 100 = 60 % functional roots. Percentage of functional roots - computation Focus group discussion for top traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked highest in preference ranking exercise Bunch weight The weight of the bunch, including the rachis. Weight bunchweight fruit curve shape (scale 1 to 6) peeper sucker blotch color scale (1 to 4) rachis appearance scale (1 to 5) The number of water suckers (suckers with broad leaves, a small rhizome, and a weak connection to the plant) in the mat. numwatersuckers Number of water suckers Number of water suckers Preference scoring scale female respondents Individual female respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. plants harvested/plants planted Leaf area ratio - computation Computation of top leaf area with total (loose) leaf area The time elapsed between a tagged cigar leaf at Bruns stage B of unrolling and the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1. DIT Black leaf streak disease incubation time Time elapsed from Bruns stage B of unrolling to Foures black leaf streak disease severity scale 1 bud length scale (1 to 3) Date of harvest - estimation Record the date of event. Date of tagged cigar leaf unrolling at Bruns stage B The date a tagged cigar leaf is at Bruns stage B of unrolling. Date of tagging at Bruns stage B of unrolling numsuckers Number of all types of suckers The number of all types of suckers in the mat. Number of suckers Finger internal length - measurement Measure the length of a finger, along the internal (ventral) arc, excluding the pedicel and the fruit tip, using a tape measure. Associate the data with the Hand rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. bract apex scale (1 to 4) sucker Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked lowest in preference ranking exercise Focus group discussion for lowest ranked traits with male respondents Bunch position - estimation Visually observe the angle of the bunch and choose the option that is most similar, from the 5 schematic drawings of descriptor 6.4.6 in the reference material. plantDryMass Dry weight The dry weight of the whole plant. Plant dry weight Adult weevil body - measurement Electronic weighing method Selection criteria for genotype ranked lowest by respondents Description of traits considered important in selection of least genotype by respondents Genotype selection traits blotch color scale (1 to 5) Visually observe the pigmentation of the outer surface of the cigar leaf of a developed sucker or on another plant of the same accession. [x Observation should be made before shooting, on a developed sucker or on another plant of the same accession.] Pigmentation of outer surface of cigar leaf - estimation Banana pest disease Individual male respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. Preference scoring scale male respondents Number of nematodes (Pratylenchus coffeae) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Preference number scale Average head capsule width The average width of a banana weevil adult head. Banana weevil adult average head capsule width rwcLeaf Content The relative water content in a leaf Leaf relative water content Matooke MatAroMatA Aroma of the local matooke. Matooke-like aroma Matooke aroma matooke plants/unit of area BD colour chart A - 2016 Percentage damage to upper cross-section outer corm caused by weevils Damage percent X-upper-outer The amount of damage to the outer corm (cortex) upper cross-section, assessed by percentage of tunnels caused by weevils. Youngest leaf spotted - counting Record the rank (order) of the youngest leaf spotted (the first fully unfurled leaf with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres), counting the rank by starting with the youngest completely unrolled leaf as 1 and moving downwards, associating the data with the Date of data collection. bud size scale (1 to 3) Mealiness Boiled plantain BoiPlantnMeal Mechanical property linked to cohesion and the presence of fine particles in the product during chewing Boiled plantain Mealiness Average actual annual yield Average actual annual yield The average actual annual yield, taking into account the proportion of plants harvested, from the number of plants planted actualannyield fruit pedicel scale (1 to 3) Pseudostem relative water content The relative water content in a pseudostem Content rwcPseudostem Count how many hands are used to obtain a measurement. Number of hands measured - counting Record the date of event. Date of first youngest leaf spotted - estimation Focus group discussion with female respondents, to discuss selection traits of genotype that was second ranked lowest in preference ranking exercise Focus group discussion for second lowest ranked traits with female respondents g Appearance of flowers/bracts on the male rachis Male rachis appearance Appearance in relation to the presence or absence of neutral, male and/or hermaphrodite flowers Index of non-spotted leaves - computation Calculate as: 100 multiplied by (Youngest leaf spotted, minus 1), divided by Number of standing leaves, e.g. 100 * (6 - 1) / 12 = 42. Rank of marked youngest leaf - counting Mark the youngest completely unrolled leaf and record its rank, which is always 1. Associate the data with the Date of data collection. Rank of oldest standing leaf Rank of oldest standing leaf The rank (order) of the oldest (most-distal) standing leaf (with an erect petiole). Number of maiden suckers Number of maiden suckers The number of maiden suckers (fully grown suckers with foliage leaves) in the mat. nummaidensuckers The average length of the fingers measured along the external (dorsal) arc, excluding the pedicel and the fruit tip. Average finger external length avefingerextlength Average external length Weight Multiple finger weight The weight of multiple fingers. multfingerweight Petiole margin colour Main colour of the petiole margin. Where the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Colour Number of nematodes (Meloidogyne spp.) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Fruit pulp colour - estimation Visually observe the fruit pulp colour and select the option in the colour chart that is most representative. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Count the number of leaves (functional only > 50% estimated green area) Leaf number - counting Number of Pratylenchus spp. in soil Number of Pratylenchus spp. per soil sample Population density Pseudostem height Height Distance from the base of the pseudostem to the emerging point of the peduncle. The number of hands in the bunch. Number of hands in bunch Number of hands numhands Time from start of crop cycle to first external disease symptoms caused by Fusarium wilt Time from start of crop cycle to appearance of external disease symptoms caused by Fusarium wilt The time elapsed from the start of the crop cycle to the appearance on the plant of any of the external disease symptoms - - yellowing leaves, splitting pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Presence/absence of splitting Presence of splitting pseudostem base caused by Fusarium wilt SP The presence/absence of a splitting pseudostem base, caused by Fusarium wilt. Average number of fingers in hand - hands - computation Calculate as: the sum of the Number of fingers in hand, divided by the Number of hands in bunch, e.g. (10 + 10 + 11 + 10 + 10 + 10 + 12 + 12 + 12 + 11 +12 + 12 + 10 + 10 + 10 + 11) / 16 = 10.8. Extent of root knot nematodes The extent of root knot nematodes, observed externally as galls on the roots. Nematode root knot galling extent Date of death datedeath The date of death of the plant. Date of death Volume System water loss The amount of water loss in the system over time waterLossSyst Tallest sucker height Height suckerheight The height of the pseudostem of the tallest sucker within the same mat as the plant (the sucker that will become the next plant with a bunch, once the bunch from the current plant is harvested) nematodes/unit of fresh root weight Calculate as: the sum of values with a Black leaf streak disease severity grade of 0. Number of leaves with black leaf streak disease severity grade 0 - computation Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. Count the number of functional roots. Number of functional roots - counting Fruit apex point - estimation Visually observe the fruit apex point and choose the option that is most similar, from the 5 photos of descriptor 6.7.6 in the reference material. The number of plants that are harvested. numharv Combined number of plants harvested Number of plants harvested Weight Finger weight fingerweight The weight of a finger. Count how many fingers are in the third hand. Number of fingers in 2nd hand - counting Date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1 The date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1. Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 The amount of damage to the inner corm (central cylinder) lower cross-section, assessed by percentage of tunnels caused by weevils. Percentage damage to lower cross-section inner corm caused by weevils Damage percent X-lower-inner Count how many fingers are in the second-most distal hand. Number of fingers in 2nd-most distal hand - counting Gauhls modification of Stovers severity scoring system (YYYY) The texture of the smashed pulp of a mature fruit Fruit smashed pulp texture Texture Distance of internal discolouration in pseudostem of tallest sucker, caused by Fusarium wilt Distance of internal discolouration in pseudostem of tallest sucker caused by Fusarium wilt For the tallest sucker, the complete corm should be removed from the soil, the roots cut off and excess soil removed. Cross-sections of the corm should be cut (using a guillotine or other suitable device) to obtain five slices of equal thickness. The upper surface of each cut section should be examined and visually evaluate the extent of the vascular discolouration. Perception of the amount of water absorbed or released by the product Boiled plantain Moistness BoiPlantnMois Boiled plantain Moistness leaf rank No. per 10g Angle of the rachis between last hand and male bud Angle of the part of the rachis between the last hand and the male bud Male rachis position Extent of internal discolouration in corm of plant caused by Fusarium wilt Extent of discolouration The extent of discolouration in the corm of the plant, caused by Fusarium wilt. A rate that expresses the number of leaves that have emerged from the pseudostem in a week. Weekly leaf emission rate LER Weekly leaf emission rate Focus group discussion for third ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked third highest in preference ranking exercise Blotches colour at the petiole base Colour of the blotches on the upper leaf sheath of the petiole base. Main colour Gram accumulated biomass per volume water transpired Transpiration efficiency - computation Measure the distance from the collar, or from the pseudostem base at the ground if the collar is not visible, to under the neck of the curved peduncle, using a measuring pole or sliding ruler. Plant height - neck of peduncle - measurement Male bud size - measurement Length and maximum diamter of male bud at harvest. Number The number of dead roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Number of dead roots petiole-margin contrast Number of plants dead - computation Calculate as: the sum of the number of values for Date of death. open answer Presence of petiole collapse caused by Fusarium wilt - estimation Visual assessment. Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked second lowest in preference ranking exercise Focus group discussion for second lowest ranked traits with male respondents Egg-laying female root knot nematode yyyy/mm/dd Finger lateral diameter fingerlatdiam Lateral diameter The lateral (side to side) diameter of a finger. Visual counting method of Weevil traps (the trap should be minimum 30 cm long). Number of adult weevil trap - counting Pseudostem to whole plant mass ratio Measure for the biomass allocation: dry mass of pseudostem versus whole plant dry mass ratio Ratio pseudostemToPlantMassRatio_dry Visually observe the colour of the internal face of the first unlifted bract. Use colour chart A and observe out of direct sunlight. Colour of bract internal face - estimation Date of first external disease symptoms caused by Fusarium wilt - estimation Record the date of event. The width of a banana weevil adult weevil head. Head capsule width Banana weevil adult head capsule width Number of fingers in outer whorl of 2nd-most distal hand - counting Count how many fingers are in the outer whorl of the second-most distal hand. Apex point Description of fruit apex Fruit apex point Dry weight - measurement 80?C, for 14 days, or longer untill dry Number of leaves with black leaf streak disease severity grade 6 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 6. leaf Nematode species The nematode species found in the banana plant root sample. Species present Number of sword suckers Number of sword suckers The number of sword suckers (suckers with narrow leaves and a large rhizome) in the mat. numswordsuckers Number of spotted leaves - counting Count how many of the standing leaves are spotted (with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres), considering all leaves in between and inclusive of, the youngest leaf and the oldest standing leaf. Plant circumference at collar - measurement Measure the circumference of the pseudostem of the plant at the collar, or from the pseudostem base at the ground if the collar is not visible. bad good Presence of yellowing leaves caused by Fusarium wilt - estimation Visual assessment. A plant weight trait which is the fresh weight of a plant Fresh weight Plant fresh weight plantFreshMass Weevil larvae body weight - measurement Measurement under microscope Focus group discussion for third lowest ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked third lowest in preference ranking exercise The ratio of dry mass of belowground (root) to abovegrund (shoot) biomass Ratio rootShootDry Ratio root to shoot Number of nematodes (Pratylenchus coffeae) per unit fresh root weight - method Count from 100 ml of soil sample nematode lifecycle stages The length of a banana weevil adult body, from the head to the tip of the abdomen. Banana weevil adult body length Body length Number of leaves with a black leaf streak disease severity grade of 2 The number of leaves on a plant with a black leaf streak disease severity grade of 2. Number of leaves with black leaf streak disease severity grade 2 Leaf area - measurement Top view image Selection traits (text) Water content - computation (Plant Fresh Weight - Plant Dry Weight) / Plant Fresh Weight Sourness Matooke Matooke sourness MatSour gustatory complex sensation, generally due to presence of organic acids Leaf length - measurement The longest diagonal of a fitted ellips Vitamin C content Vitamin C content The Vitamin C content of freeze-dried smashed pulp of a mature fruit BD colour chart B - 1996 The weight of a banana weevil larvae body. Body weight Banana weevil larvae body weight The presence or absence of dwarfism on a mat Dwarfism of plants dwarfism Dwarfism Count how many fingers are in the third hand. Number of fingers in 3rd hand - counting Index of non-spotted leaves Count how many maiden suckers are in the mat. Number of maiden suckers - counting Visual estimation of fruit pendulance on a bunch, for Early Evaluation Trial stage only. Fruit pendulance - estimation The average circumference of the fingers. avefingercircumf Average circumference Average finger circumference Record the date and time of event. End date and time of data collection - estimation Fruit smashed pulp taste Taste The taste of the smashed pulp of a mature fruit Cause of death - estimation Record the apparent cause of death of the plant, e.g. strong winds, Fusarium wilt, etc. Cut multiple hands from the rachis and weigh them together, using scales. Associate the data with the Hank rank and the Number of hands measured. Multiple hand weight - measurement Average finger internal length - computation Calculate as: the sum of the Finger internal length measurements, divided by the number of those measurements, e.g. (27 + 31 + 29 + 30 + 30 + 28) / 6 = 29.2. Compound tepal main colour Main colour of the backside of the compound tepal Main colour Weight The weight of multiple hands. Multiple hand weight multhandweight Finger feel assessment of smashed and unsmashed fruit pulp Finger feel - estimation The number of plants that are planted. Number of plants planted numplanted Combined number of plants planted Number of Helicotylenchus multicinctus in soil Number of Helicotylenchus multicinctus per soil sample Population density Growth Leaf area increase over time Increase of leaf area leafAreaIncrease Visually observe the colour of the external face of the first unlifted bract. Use colour chart A and observe out of direct sunlight. Colour of bract external face - estimation Collect all roots from a standard size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Carefully wash the soil from the roots with tap water. Cut the roots into 1 cm pieces. Take a subsample of 5 g, add 100 ml distilled water and store the roots at 4 degrees Celsius. Stain the egg masses by immersing the roots in 0.15 g/L phloxine B for 15 mins. Count the number of egg-laying females under a stereo microscope. Nematode egg-laying females extent - estimation leaves/month Time from harvest to death The time elapsed from the harvest of the bunch of the plant from the preceding crop cycle to the death of the plant from the current crop cycle. Time from harvest to death harvest2death stickiness scale 0-10 Bract behaviour before falling - estimation Visually observe the last bract to lift from the hands, ideally when the bract has lifted to a horizontal position, and choose the option that is most similar, from the 2 photos of descriptpr 6.5.12 in the reference material. Time from shooting to harvest The time elapsed from shooting (when the inflorescence emerges from the pseudostem and is still in an erect position) to the harvest of the bunch. shoot2harvest Time from shooting to harvest Description of damage describedamage A description of the damage to the plant. Damage description Chewiness Colour line along edge of petiole margin - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Record on the last developed leaf at [x flowering stage] [shooting]. Number of suckers - counting Count how many of all types of suckers are in the mat. parthenocarpy plant2death Time from planting to death Time from planting to death The time elapsed from planting to the death of the plant. Bract imbrication at apex of male bud - estimation Visually observe the bract imbrication of the apex of the male bud and choose the option that is most similar, from the 3 photos of descriptor 6.5.3 in the reference material. Boiled plantain Boiled plantain Stickiness Force required to peel off the fraction of product adhering to the interior of the oral cavity Stickiness BoiPlantnStic Cut a transverse cross section of the corm at the collar (upper cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Damage to upper inner corm weevils - measurement The area allocated to one plant. Plant spacing plantspacing Area allocated to one plant Leaf area - measurement Top view image of a single leave Calculate as: the sum of the Number of fingers in 3rd hand and the Number of fingers in 2nd-most distal hand, divided by 2, and multiplied by the Number of hands in bunch, e.g. (16 + 12) / 2 * 8 = 112. Number of fingers in bunch - estimation The number of all leaves (including dried/dead/wilted/yellow) in a shoot system Leaf number Number numLeaves topLeafArea The projected leaf area of a plant (photosynthetic surface) Projected top area Area Percentage of functional roots The percentage of all roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant that are functional. Percentage of functional roots Presence absence 0 The number of leaves on a plant with a black leaf streak disease severity grade of 3. Number of leaves with black leaf streak disease severity grade 3 Number of leaves with a black leaf streak disease severity grade of 3 Fresh weight - measurement Weighing avenumfingshand Average number of fingers The average number of fingers in a hand. Average number of fingers in hand 5 pt corm discolouration scale Male bud length Length of male bud from end of rachis to apex of the bud Length Shape Shape of male bud Male bud shape finger Average finger internal length The average length of the fingers measured along the internal (ventral) arc, excluding the pedicel and the fruit tip. avefingerintlength Average internal length Total finger weight - computation Calculate as: the Bunch weight, minus the Rachis weight, e.g. 50 - 10 = 40. Matooke Astringency Matooke astringency complex sensation, accompanied by shrinking, drawing or puckering of the skin or mucosal surface in the mouth, produced by substances such as kaki tannins or sloe tannins MatAstr The number of plants that are alive but not harvested (e.g. due to slow or poor development). Number of plants not harvested numnotharv Combined number of plants not harvested Banana weevil larvae average body length Average body length The average length of a weevil larvae body, from the head to the tip of the abdomen. Nematode multiplication from single root inoculation - computation Calculation from count Sourness method Put a part of the sample in the mouth and evaluate the intensity of the sourness waterLossDay Daily rate of water loss The average daily water loss Rate leafChlorosis Leaf trait correlated to the health stage of the leaf Leaf chlorosis Chlorosis height score (1 to 3) Volume waterLossSystPerLeafArea System water loss per leaf area The amount of water loss in the system normalized by the leaf area Vitamin C - measurement Calculate as: the sum of the number of values for Presence of petiole collapse caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 84 / 300 * 100 = 28%. Hand rank of 2nd-most distal hand handid2ndmostdistal The rank (order) of a the second-most distal hand in the bunch. Rank Distance of internal discolouration in pseudostem of plant caused by Fusarium wilt For the plant, the complete corm should be removed from the soil, the roots cut off and excess soil removed. Cross-sections of the corm should be cut (using a guillotine or other suitable device) to obtain five slices of equal thickness. The upper surface of each cut section should be examined and visually evaluate the extent of the vascular discolouration. Black leaf streak disease severity BLS An indication of the extent of damage on an individual leaf caused by black leaf streak disease. Black leaf streak disease severity BD colour chart A - 1996 Time from planting to shooting - computation Calculate as: the time elapsed between the Date of planting and the Date of shooting. E.g. 24/09/2014 - 24/01/2014 = 243 days. Calculate as: the Number of plants dead, divided by the Number of plants planted, e.g. 4 / 24 = 0.17. Death proportion - computation Number of nematodes (Pratylenchus spp.) per unit fresh root weight - method Count from 100 ml of soil sample Growth - computation Dry weight difference between timepoints (refer to experimental metadata) plants not harvested/plants planted The weight of a hand. handweight Weight Hand weight Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 and the Date of tagged cigar leaf unrolling at stage B, e.g. 24/04/2014 - 24/03/2014 = 31 days. Black leaf streak disease incubation time - computation datestarttimedatacoll Date and time of start of data collection Start date and time of data collection The date and time of the start of data collection on a plant. Main colour of the first external unlifted bract internal face Colour of bract internal face Colour of the internal face The shortest diagonal of a fitted ellips Leaf width - measurement moldability scale 0-10 The length of a leaf Total length of all leaves totLeafLength Length The apparent cause of damage to the plant. Cause of damage causedamage Cause of damage Bunch quality - estimation Visual estimation of bunch quality, for Early Evaluation Trial stage only. variable_of Weighing of water loss though system normalized by leaf area Water loss - measurement Number of standing leaves - counting Count how many of the leaves are standing leaves (without a bent or broken petiole) Calculate as: the Bunch weight divided by 1,000, multiplied by the Annual crop cycle proportion, multiplied by the Plant density, e.g. (50 / 1,000) * 1.09 * 1,667 = 90.85. Potential annual yield - computation The date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6. Date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6 Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 fusion of pedicel scale (1 to 2) Smoothness-mouth feel The smoothness-mouth feel of the unsmashed pulp of a mature fruit Fruit unsmashed pulp smoothness-mouth feel Percentage of plants with a splitting pseudostem base, caused by Fusarium wilt. Combined percentage with splitting pseudostem base Percentage of plants with a splitting pseudostem base caused by Fusarium wilt Dry weight rootDryMass The dry weight of the root system Root dry weight Date of harvest The date the bunch is harvested from the plant. Date of harvest dateharvest Weighing of water loss though system between timepoints (refer to experimental metadata) Water loss - measurement Number of adult weevils - counting Visual counting method of Weevils per trap (the trap should be minimum 30 cm long) Smoothness-Mouth feel - estimation Mouth feel assessment of smashed and unsmashed fruit pulp Length of the internal arc of a finger ** subsume Length [x at maturity] Fruit length Calculate as: the time elapsed between the Date of harvest of the bunch of the plant from the preceeding crop cycle and the Date of death of the current plant, e.g. 24/03/2015 - 24/12/2014 = 90 days. Time from harvest to death - computation Bract behaviour before falling Behaviour before falling, whether revolute or not revolute Behaviour of the last lifted bract - revolute or not revolute Surface color homogeneity Appearance method When you receive the sample, observe the surface and evaluate the homogeneity MatFirmM Matooke Matooke firmness Mechanical textural attribute relating to the force required to achieve a given deformation, penetration, or breakage of a product. Firmness in mouth Leaf chlorosis - computation Ratio of non green to green plant pixels Population density Number of Pratylenchus goodeyi per fresh root weight Number of Pratylenchus goodeyi in roots Description of traits considered important in selection of second least genotype by respondents Selection criteria for genotype ranked second lowest by respondents Genotype selection traits BD colour chart A - 1996 Fruit length - range - measurement Measure the length of the internal arc of a fruit, without pedicel. Record on the inner fruit in the middle of the mid-hand of the bunch. If there is an even number of hands, there will be two middle hands so use the upper hand that developed first. Record the range. leaves/week Fruit unsmashed pulp taste The taste of the unsmashed pulp of a mature fruit Taste Fruit pulp lateral diameter - measurement Cut a finger in half, crosswise at its widest point, and on the cross-section measure the lateral diameter of the fruit pulp, from the left to the right side (not from the ventral to the dorsal side), using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. BD colour chart B - 2016 Number of leaves with black leaf streak disease severity grade 4 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 4. Visually observe the male rachis appearance and choose the option that is most similar. Male rachis appearance - estimation Rachis weight - measurement Cut the hands off the rachis and weigh the rachis, using scales. hardness scale 0-10 Description of traits considered important in selection of third least genotype by respondents Genotype selection traits Selection criteria for genotype ranked third lowest by respondents Put a part of the sample in your mouth, evaluate during the first bite (between molars) how hard is the sample. Firmness in mouth method Number used to obtain a measurement numfingersmeas The number of fingers used to obtain a measurement. Number of fingers measured Blotches colour - estimation Observe visually the colour of the blotches on the upper leaf sheath. Researchers calculate the preference score by respondents Preference score individual respondents wing presence/absence Average damage to the corm weevils - computation Calculate as: the sum of Damage to inner corm upper cross-section caused by weevils, Damage to outer corm upper cross-section caused by weevils, Damage to inner corm lower cross-section caused by weevils, and Damage to outer corm lower cross-section caused by weevils, divided by the number of traits included in the sum, e.g. (25 + 36 + 18 + 20) / 4 = 24.8. rachis type scale (1 to 2) hand fruit relicts scale (1 to 3) INSL Index of non-spotted leaves An index to express the proportion of standing leaves without the typical late-stage symptoms of black leaf streak disease, i.e. a black spot with a necrotic centre. This index provides an estimation of available photosynthetic leaf area prior to fruit filling and is a measure of resistance. It also corrects for the difference in the number of leaves produced by different types of bananas. Index of non-spotted leaves Aroma of fresh grass Grassy-like aroma Matooke aroma grassy MatAroGrassA Matooke The overall estimation of fruit parthenocarpy level on a bunch Parthenocarpy Parthenocarpy goodfruitfill Presence/absence of collapse The presence/absence of petiole collapse, caused by Fusarium wilt. PC Presence of petiole collapse caused by Fusarium wilt Number of dead roots - counting Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. Count the number of dead roots. Main colour of the face of anther opposite to dehiscence Colour Anther colour Blotch surface area Percentage coverage of petiole base by blotches. Blotches relative area at petiole base Visually observe the shape of the mature bunch on a fully developed plant that is not experiencing environmental stress and choose the option that is most similar, from the 4 photos of descriptor 6.4.7. in the reference material. Bunch shape - estimation bract imbrication scale (1 to 3) roots potannyield Average potential annual yield Average potential annual yield The average potential annual yield, assuming that all plants are harvested, from the number of plants planted. Harvest the bunch by cutting the peduncle 10 cm above the first ridge above the most proximal, hand and immediately below the most distal hand and weigh the bunch (including the rachis), using scales. Bunch weight - measurement Population density Nematode reproduction factor (microplot screening) Nematode multiplication from single root inoculation of 50 nematodes per 8 cm length after 12 weeks 80?C, for 14 days, or longer untill dry Dry weight - measurement Describe the pest and/or disease assessment and/or sample collection taking place at the time of data collection. Pest and/or disease assessment and/or sample collection description - estimation Visually observe the male rachis position (the part between the last hand and the male bud) and choose the option that is most similar, from the 5 schematic drawings of descriptor 6.4.12 in the reference material. Male rachis position - estimation Put a part of the sample in mouth, chew it and after 5 chews, evaluate between tongue and palate the number and the size of the particles. Smoothness in mouth method Number of nematodes (Meloidogyne spp.) per unit fresh root weight - method Count from 100 ml of soil sample numfingers3rdhand The number of fingers in the third hand. Number of fingers in 3rd hand Number of fingers Leaf area to plant area ratio leafAreaRatio Ratio Ratio of projected leaf area to the actual leaf area, measure for the compactness of the plant crown Tallest sucker height - measurement On the tallest sucker, measure the distance from the pseudostem base at the ground to the intersection of the petioles of the two youngest leaves (leaf ranks 1 and 2), using a measuring pole or sliding ruler. Fresh plant growth growthFresh Growth Fresh mass accumulation over time Colour of pigmentation on outer surface of cigar leaf on a developed sucker Pigmentation of outer surface Pigmentation of outer surface of cigar leaf on sucker water suckers Focus group discussion for third ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked third highest in preference ranking exercise Number of fingers estnumfingers The estimated number of fingers in the bunch based on a calculation using the number of fingers in the third and second-most distal hands and the number of hands in the bunch. Calculated number of fingers in bunch Visual assessment. Presence of changes in new leaves caused by Fusarium wilt - estimation bud shoulder scale (1 to 3) fruit curve shape (scale 1 to 5) Record the date of event. Date of death - estimation Presence absence 1 Visual assessment. Presence of splitting pseudostem base caused by Fusarium wilt - estimation Average fruit pulp lateral diameter The average lateral (side to side) diameter of the pulp of a fruit. avepulpdiam Average lateral diameter of the pulp Plant relative water content Content The relative water content in the whole plant rwcPlant Number of nematodes (Pratylenchus goodeyi) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Date of flowering - estimation Record the date of event. Rank Hand rank The rank (order) of a hand in the bunch. handid hand rank The height of the pseudostem of the plant, from the collar to the intersection of the petioles of the two youngest leaves. Height Plant height plantheightpetioles Presence absence 0 Count how many water suckers are in the mat. Number of water suckers - counting Astringency method Put a part of the sample in the mouth and evaluate the intensity of astringency impression due to the sample plantcircumf20cm The circumference of the pseudostem of the plant at 20 cm from the collar. Circumference 20 cm above the ground Plant circumference 20 cm from the collar Date of data collection - estimation Record the date of event. The number of leaves on a plant with a black leaf streak disease severity grade of 5. Number of leaves with black leaf streak disease severity grade 5 Number of leaves with a black leaf streak disease severity grade of 5 bud shape scale (1 to 5) Stickiness Sensory Texture method Press a piece of banana between the molars and appreciate the adhesion of the product Date of recognising off-type dateofftype The date the plant is first recognised as an off-type. Date of recognising off-type Average finger lateral diameter - computation Calculate as: the sum of the Finger lateral diameter measurements, divided by the number of those measurements, e.g. (3.5 + 5.2 + 4.1 + 5 + 6 + 6.8) / 6 = 5.1. Assessment by respondents of physical features of a plant by giving it a score on the preference scale Overall plant appearance Overall plant appearance PA kg Meredith and Lawrence (1969) - complete Water content - computation (Root Fresh Weight - Root Dry Weight) / Root Fresh Weight Number of fingers The number of fingers in multiple hands. Multiple number of fingers in hand multnumfingshand Measure the length (y) of a projection/outline of the male bud on paper, with a measuring tape or ruler. Male bud length - measurement The average length of a banana weevil adult body, from the head to the tip of the abdomen. Average body length Banana weevil adult average body length Leaf yellowing and wilting caused by Fusarium wilt rating A rating of the extent of leaf yellowing and wilting, caused by Fusarium wilt. Rating of external symptoms of Fusarium wilt in the field Number of nematodes in soil Nematode population density by species in soil Population density Calculate as: the sum of the number of values for Presence of splitting pseudostem base caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 24 / 300 * 100 = 8%. Percentage of plants with a splitting pseudostem base caused by Fusarium wilt - computation Number used to obtain a measurement Number of hands measured The number of hands used to obtain a measurement. numhandsmeas The dry weight of the pseudostem Dry weight pseudostemDryMass Pseudostem dry weight DDT Black leaf streak disease development time The time elapsed between a tagged cigar leaf at Bruns stage B of unrolling and the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6. Time elapsed from Bruns stage B of unrolling to Foures black leaf streak disease severity scale 6 moisture scale 0-10 Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 - estimation Record the date of event. Moisture in mouth Matooke moisture perception of moisture content of a food by the tactile receptors in the mouth and also in relation to the lubricating properties of the product Matooke MatMoistM finger Shape of a fruit (finger) in the middle of the bunch **subsume Shape of the longitudinal curvature Fruit shape of curve mm Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked second highest in preference ranking exercise Focus group discussion for second ranked traits with female respondents Meredith and Lawrence (1969) - condensed Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked second highest in preference ranking exercise Focus group discussion for second ranked traits with male respondents Male bud shoulder Male bud shoulder description Ratio Refers to the first external unlifted bract. Visually observe the apex shape of flattened bracts to determine shape and choose the option that is most similar, from the 4 photos of descriptor 6.5.2. in the reference material. Bract apex shape - estimation Number The number of functional roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Number of functional roots Calculate as: the Rank of previously marked leaf at one point in time, minus 1 (equivalent to the Rank of marked youngest leaf), divided by the time elapsed between the two Date of data collection events when the marked leaf was recorded as 1) the Rank of marked youngest leaf and 2) the Rank of previously marked leaf, e.g. (4 - 1) / 1 month = LER of 3 leaves/month during a particular time period. Monthly leaf emission rate - computation Open question Number of standing leaves Number of standing leaves The number of leaves attached to the plant that have an erect petiole (not bent or broken). Firmness Sensory Texture method Put in the mouth a piece of banana and Evaluate the force necessary to obtain the deformation of the product between the teeth during the first compression lengthPseudostem Pseudostem length Length The length of a pseudostem firmness scale 0-10 Calculate as: the sum of the number of values for Presence of petiole collapse caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 84 / 300 * 100 = 28%. Percentage of plants with petiole collapse caused by Fusarium wilt - computation 2 pt scale rachis angle scale (1 to 2) Annual crop cycle proportion Proportion of crop cycle that takes place in one year The proportion of the crop cycle that takes place in one year. anncropcycprop Researchers calculate the preference score by female respondents Preference score female respondents mm Number of fingers in 2nd-most distal hand The number of fingers in the second-most distal hand Number of fingers numfingers2nddistalhand Damage to lower outer corm weevils - measurement Cut a transverse cross section of the corm at 10 cm below the collar (lower cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Ratio - computation Ratio of leaf dry mass versus plant dry mass Visual assessment. Rating of external symptoms of Fusarium wilt in the field - estimation None Index of black leaf streak disease severity Calculate as: the length of a year (e.g. 365 days) divided by the Plant crop cycle or the Ratoon crop cycle, e.g. 365 / 334 = 1.09. Annual crop cycle proportion - computation Record the rank (order) of the second-most distal hand in the bunch, starting with the hand at the proximal end (closest to the pseudostem) of the bunch as 1 and continuing to the hand at the most distal end (closest to the male bud). Hand rank - counting Plant annual yield plantannyield Annual yield The bunch weight produced by the plant over the course of the crop cycle, averaged across one year. Count only fully developed fruit. If there is an even number of hands, there will be two middle hands. Count the middle hand that developed first. Number of fruits on mid-hand of the bunch - counting Number of leaves with black leaf streak disease severity grade 1 Number of leaves with a black leaf streak disease severity grade of 1 The number of leaves on a plant with a black leaf streak disease severity grade of 1. Preference scale The date a youngest leaf spotted is first observed on the plant, caused by black leaf streak disease. Date of first youngest leaf spotted Date of first youngest leaf spotted The lateral (side to side) diameter of the pulp of a fruit. Lateral diameter of the pulp Fruit pulp lateral diameter pulpdiam Weighing of water loss though plant between timepoints (refer to experimental metadata) Water loss - measurement bract behaviour scale (1 to 2) astringency scale 0-10 Visual counting method Number of adult larvae - counting Fusarium wilt external symptoms scale Faures stages of development of BLS disease scale (1987) rwcRoot The relative water content in a root Root relative water content Content The percentage of the root cortex of five functional roots approximately 10 cm long that is necrotic due to nematode damage. Percentage necrotic Root necrosis caused by nematodes sword sucker sweetness scale 0-10 Harvest proportion - computation Calculate as: the Number of plants harvested, divided by the Number of plants planted, e.g. 18 / 24 = 0.75. Average number of banana weevil adults per trap Average number of banana weevil adults The average of how many adult banana weevils caught per trap. A leaf weight trait which is the fresh weight of a leaf leafFreshMass Leaf fresh weight Fresh weight The smoothness-mouth feel of the smashed pulp of a mature fruit Smoothness-mouth feel Fruit smashed pulp smoothness-mouth feel Bunch position Angle between the axis of the bunch and the vertical Angle to the vertical Number of hands on whole bunch Number of hands Number of hands on bunch **subsume Dry weight - measurement 80?C, for 14 days, or longer untill dry Count how many sword suckers are in the mat. Number of sword suckers - counting Pest/disease assessment/sample collection options The options to record the pest and/or disease assessment and/or sample collection done at the time of data collection. Pest and/or disease assessment and/or sample collection options pestdiseasesampleoptions Alignment of bracts at the apex of the male bud Imbrication Bract imbrication at apex of male bud Number of functional leaves - counting Count how many functional leaves (leaves that have 50% or more of their surface as green, healthy, photosynthetic tissue) are on the plant. fruit length scale (1 to 5) functional roots bunchmaturity The stage of maturity of the bunch at harvest. Stage of maturity at harvest Bunch maturity stage at harvest The percentage of all roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant that are dead. Percentage of dead roots Percentage of dead roots Death proportion The proportion of plants that are dead, from the number of plants planted. deathprop Combined proportion of plants that are dead Preference score male respondents Researchers calculate the preference score by male respondents sourness scale 0-10 clasping or not clasping 80?C, for 14 days, or longer untill dry Dry weight - measurement Date of tagged cigar leaf unrolling at Bruns stage B - estimation Record the date of event. scale_of Damage percent X-average Average percentage damage to the corm caused by weevils The average percentage of damage in the whole corm (the inner corm and the outer corm) caused by weevils. Selection criteria for genotype ranked second highest by respondents Genotype selection traits Description of traits considered important in selection of second best genotype by respondents Count from 100 ml of soil sample Number of nematodes (Radopholus similis ) per unit fresh root weight - method Main colour of the first external unlifted bract external face Colour of bract external face Colour of the external face Leaf length - measurement The longest diagonal of a fitted ellips Percentage of plants with changes in new leaves caused by Fusarium wilt Percentage of plants with changes in new leaves, caused by Fusarium wilt. Combined percentage with changes in new leaves totalfingerweight Combined weight The weight of all the hands of the bunch, excluding the rachis. Total finger weight Measure the distance from the collar, or from the pseudostem base at the ground if the collar is not visible, to the intersection of the petioles of the two youngest leaves (leaf ranks 1 and 2), using a measuring pole or sliding ruler. Plant height - petiole of two youngest leaves - measurement Put a part of the sample in the mouth and evaluate the intensity of taste of sweetness Sweetness tasting method Leaf area The area of a leaf singleLeafArea Area hands Calculate as: the Plant density multiplied by the Death proportion, e.g. 1,667 * 0.17 = 283. Death density - computation Pest and/or disease assessment and/or sample collection options - estimation Record the pest and/or disease assessment and/or sample collection taking place at the time of data collection. Banana weevil larvae average head capsule width Average head capsule width The average width of a banana weevil larvae head capsule. Banana weevil larvae body length Body length The length of a weevil larvae body, from the weevil larvae head to the tip of the abdomen. How many Number of banana weevil traps used How many banana weevil traps used. Number of Hoplolaimus spp. per soil sample Population density Number of Hoplolaimus spp. in soil Individual respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. Preference scoring scale individual respondents Date of planting Date of planting The date of planting the plant. dateplant The width of a weevil larvae head capsule. Head capsule width Banana weevil larvae head capsule width Off-type description - estimation Record a description of what made the plant recogniseable as an off-type. Leaf area increase between timepoints (refer to experimental metadata) per number of new leaves formed between timepoints (refer to experimental metadata) Growth - computation Measurement of system water loss between two timepoints (refer to experimental metadata) Water loss rate - computation Leaf number - counting Count the number of leaves Identify the nematode stage, using the options. Nematode stage - estimation Count from 100 ml of soil sample Number of nematodes (Pratylenchus goodeyi) per unit fresh root weight - method Functional leaf number Number The number of functional leaves (greenness>50%) in a shoot system numFuncLeaves Harvest density - computation Calculate as: the Plant density multiplied by the Harvest proportion, e.g. 1,667 * 0.75 = 1,250. The extent of discolouration in the corm of the tallest sucker, caused by Fusarium wilt. Extent of discolouration Extent of internal discolouration in corm of tallest sucker caused by Fusarium wilt Record the apparent cause of the damage to the plant, e.g. strong winds, weevil damage, etc. Cause of damage - estimation Estimate maturity stage of the bunch. Bunch maturity stage - estimation Number of peeper suckers - counting Count how many peeper suckers are in the mat. Mealiness Sensory Texture method Put a piece of banana in your mouth and assess the presence of mealiness particles during chewing Place the sample in the mouth, chew it at the rate of one chewing per second and assess the number of chews before swallowing. Chewiness Sensory Texture method Nematode root knot galling extent - estimation Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. From the functional roots, randomly select five primary roots that are at least 10 cm, and reduce their length to 10 cm. Where root knot nematode galls are present, determine the extent of the galling using the scale. flower2harvest The time elapsed from flowering (when the last bracts fall from the most distal hand to display the female flowers) to the harvest of the bunch. Time from flowering to harvest Time from flowering to harvest Number of plants not harvested - computation Calculate as: the Number of plants planted, minus the Number of plants dead, minus the Number of plants harvested, e.g. 24 - 4 - 18 = 2. Calculate the ratio w/y, i.e. the broadest width of the male bud to the total length of the male bud. Do not measure the dimensions along the bud but rather on a projection/outline of the bud (e.g. trace the outline of the bud on paper) and use ruler or measuring tape Male bud shoulder - computation Number of fingers in outer whorl numfingersouterwhorl2nddistalhand Number of fingers in outer whorl of 2nd-most distal hand The number of fingers in the outer whorl of the second-most distal hand Number of all roots - computation Calculate as: the Number of dead roots plus the Number of functional roots, e.g. 8 + 12 = 20 roots. Plant density - computation Calculate as: number of square metres in the area unit of choice, divided by the Plant spacing, e.g. 10,000 square metres in 1 hectare / 6 = 1,667 plants/ha. Firmness scale 0-10 The number of plants that are dead. Combined number of plants that are dead numdead Number of plants dead wing presence/absence and clasping presence/absence scale 1 to 5 Growth - computation Pseudostem height difference between timepoints (refer to experimental metadata) Number of Meloidogyne spp. per fresh root weight Number of Meloidogyne spp. in roots Population density Recorded from the base of the pseudostem to the emerging point of the peduncle. Pseudostem height - estimation The circumference of the pseudostem of the plant at 75 cm from the collar. Plant circumference 75 cm from the collar Circumference 75 cm above the ground plantcircumf75cm Number of peeper suckers The number of peeper suckers (suckers less than 15 cm tall) in the mat. numpeepersuckers Number of peeper suckers Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Use colour chart A and observe out of direct sunlight. Record the colour of the margin (general colour is below the rim). [x Observe at flowering time.] Petiole margin colour - estimation Cut a finger in half, crosswise at its widest point, and on the cross-section measure the width of the peel, using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger peel thickness - measurement BD colour chart A - 2016 Date of planting - estimation Record the date of event. bunch maturity []/cm Calculate as: the Bunch weight divided by 1,000, multiplied by the Annual crop cycle proportion, multiplied by the Harvest density, e.g. (50 / 1,000) * 1.09 * 1,250 = 68.13. Actual annual yield - computation Calculate as: the sum of the Fruit pulp lateral diameter measurements, divided by the number of those measurements, e.g. (3.1 + 4.8 + 3.7 + 4.6 + 5.6 + 6.4) / 6 = 4.7. Average fruit pulp lateral diameter - computation Cause of death The cause of death of the plant. causedeath Cause of death Margins clasping or not clasping Behaviour of the petiole margins - winged or not winged. the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Petiole margins clasping Moistness scale 0-10 Surface yellowness intensity Appearance method When you receive the sample, observe the surface and evaluate the intensity of the color Number of fingers The number of fingers in a hand. Number of fingers in hand numfingershand Number of fingers Number of fruits on the mid-hand of the bunch **subsume Number of fruits on mid-hand of the bunch Hardness in hand method Take a part of the sample between fingers and evaluate how hard the sample is Male rachis type - estimation Visually observe the part of the rachis between the last hand and the male bud. Truncated means there is no bract scar below the last hand. Present means there is a degenerated or persistent male bud Growth - computation Leaf area difference between timepoints (refer to experimental metadata) mL / cm? Record the date of event. Date of recognising off-type - estimation Average thickness of the peel Average finger peel thickness The average thickness of the peel of a finger. avepeelthickness Calculate as: the sum of values with a Black leaf streak disease severity grade of 2. Number of leaves with black leaf streak disease severity grade 2 - computation Annual plant productivity - computation Calculate as: the Bunch weight multiplied by the Annual crop cycle proportion, e.g. 48 * 1.09 = 52.32. Calculate as: the sum of the number of plants with any or multiple of the external disease symptoms caused by Fusarium wilt - yellowing leaves, splitting of pseudostem base, changes in new leaves, petiole collapse - divided by the Number of plants planted * 100, e.g. 112 / 300 * 100 = 37%. Percentage of plants with external disease symptoms caused by Fusarium wilt - computation Finger circumference - measurement Measure the circumference of a finger at its widest point, using a tape measure. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Date of damage - estimation Record the date of event. Index of black leaf streak disease severity t/unit of area/y A description of the state of the plant. statedescription Plant state description State description kg Number of banana weevil adults per trap How many banana weevil adults caught per trap. Number of banana weevil adults The total number of all roots (both dead and functional) in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Number of all roots Number Record the date of event. Date of shooting - estimation Number of nematodes (unclassified spp.) per unit fresh root weight - method Count from 100 ml of soil sample Number of Radopholus similis per soil sample Population density Number of Radopholus similis in soil Select at random five functional primary roots, at least 10 cm long. Reduce the length of the five selected roots to 10 cm and slice through the roots length-wise. Evaluate one half of each of the five roots for the percentage of root cortex showing necrosis. The maximum root necrosis per root half can be 20 %, giving a maximum root necrosis of 100 % for the five halves together. Mentally determine the necrosis of the individual roots (1 to 5) and sum these together to get the total root necrosis of the sample. Root necrosis caused by nematodes - estimation Calculate as: the time elapsed between the Date of shooting to the Date of harvest. E.g. 24/12/2014 - 24/09/2014 = 91 days. Time from shooting to harvest - computation Focus group discussion for third lowest ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked third lowest in preference ranking exercise Date of data collection Date of data collection The date of data collection. datedatacoll The amount of damage to the inner corm (central cylinder) upper cross-section, assessed by the percentage of tunnels caused by weevils. Damage percent X-upper-inner Percentage damage to upper cross-section inner corm caused by weevils Number of nematodes (Hoplolaimus spp.) per unit fresh root weight - method Count from 100 ml of soil sample leaf margin behaviour scale (1 to 5) Number of Pratylenchus goodeyi per soil sample Population density Number of Pratylenchus goodeyi in soil fruit relicts scale (1 to 4) Extent of internal discolouration in corm of tallest sucker caused by Fusarium wilt - estimation The extent to which vascular discolouration extends up the pseudostem of the tallest sucker should be determined by making cross-section cuts, from the base of the pseudostem upwards, and examining the internal tissues following each cut. The point at which discolouration is no longer visible should be noted and the distance from this point to the pseudostem base recorded. Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 and the Date of tagged cigar leaf unrolling at stage B, e.g. 24/06/2014 - 24/03/2014 = 92 days. Black leaf streak disease development time - computation Plant circumference at collar The circumference of the pseudostem of the plant at the collar. plantcircumfcollar Circumference at collar Population density Number of Hoplolaimus spp. per fresh root weight Number of Hoplolaimus spp. in roots The date the assessment of weevil damage to the corm of the plant is conducted. Date of weevil damage assessment Date of weevil damage to corm assessment dd/mm/yyyy hh:mm:ss Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. From the functional roots, randomly select five primary roots that are at least 10 cm, and reduce their length to 10 cm. Determine the presence/absence of root knot nematodes, observed externally as galls on the roots. Nematode root knot galling presence - estimation A rating of the extent of rhizome (corm) discolouration, caused by Fusarium wilt. Rating of internal symptoms of Fusarium wilt in the greenhouse Discolouration caused by Fusarium wilt rating method_of The time elapsed between the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1, to 6. DET Time elapsed from Foures black leaf streak disease severity scale 1 to 6 Black leaf streak disease evolution time Pumpkin-like Aroma method Put a part of the product and by retro-olfaction evaluate the presence and the intensity of pumpkin-like aroma Ratoon crop cycle - computation Calculate as: the time elapsed between the Date of harvest of the bunch of the plant from the preceeding crop cycle and the Date of harvest of the bunch of the plant from the current crop cycle, e.g. 24/11/2015 - 24/12/2014 = 335 days. Calculate as: the sum of the Finger external length measurements, divided by the number of those measurements, e.g. (30 + 34 + 32 + 33 + 33 + 31) / 6 = 32.1. Average finger external length - computation yellowness scale 0-10 Population density Number of Pratylenchus coffeae per fresh root weight Number of Pratylenchus coffeae in roots Number of leaves with black leaf streak disease severity grade 3 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 3. fruit apex scale (1 to 5) blotch pigmentation scale (1 to 4) Visual assessment. Presence of petiole collapse caused by Fusarium wilt - estimation Visual estimation of suckering, for Early Evaluation Trial stage only. Sucker quality - estimation Colour The color of the unsmashed pulp of a mature fruit Fruit unsmashed pulp colour Record a description of the damage to the plant, e.g. leaves ripped, pseudostem snapped, plant toppled, etc. Damage description - estimation The percentage of damage to the periphery of the peeled corm, assessed by tunnels caused by weevils Damage to periphery of peeled corm caused by weevils Peripheral damage caused by weevils Population density Number of Radopholus similis per fresh root weight Number of Radopholus similis in roots leaf rank The number of leaves on a plant with a black leaf streak disease severity grade of 6. Number of leaves with a black leaf streak disease severity grade of 6 Number of leaves with black leaf streak disease severity grade 6 Visual observation Peripheral damage - measurement Number of leaves with a black leaf streak disease severity grade of 0 Number of leaves with black leaf streak disease severity grade 0 The number of leaves on a plant with a black leaf streak disease severity grade of 0. Number of Pratylenchus coffeae in soil Number of Pratylenchus coffeae per soil sample Population density margin behaviour scale (1 to 4) Nosal smell - estimation Nosal smell assessment of smashed and unsmashed fruit pulp aromas Record the date and time of event. Start date and time of data collection - estimation Shape of the fully developed bunch on a plant Shape Bunch shape Number of plants harvested - computation Calculate as: the sum of the number of values for Date of harvest. Moistness Mouth impression method Once the flavors are detected, moisture is felt by turning the product in the mouth