IITA-Bioversity International BlkLfStrkSvrt_Est_Gauhl grey 4 5 obtuse and split Guillaume Bauchet FrtDiam_M_cm IITA-BTI Rhiannon Crichton DateDeath_Est_dmy Bioversity International SpotLfNum_Ct_Lf Amos Alakonya-Rhiannon Crichton IITA-Bioversity International banana streak virus (BSV) 5 Danny Coyne IITA NmRpFctr_Ct_Nema Rhiannon Crichton temp35 Bioversity International FstExtDiSmptFW_Date_dmy Bioversity International Rhiannon Crichton 5 discolouration greater than two-thirds of vascular tissue Rhiannon Crichton FnctLeav_Ct_Leaf Bioversity International BlkLfStrkSvrt_Est_pct IITA-Bioversity International Rhiannon Crichton PStmGrth_M_cm Bioversity International 6 pink/pink-purple HndMltpFrtNum_Ct_Frt Rhiannon Crichton Bioversity International Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd1 Bioversity International 4 margins overlapping Rhiannon Crichton RkYgstLf_Comp_leafrank Bioversity International 0 no intensity Rhiannon Crichton PltDamDesc_Est_txt Bioversity International Bioversity International PtYd_Comp_TnHaY Rhiannon Crichton CrmIntDiscFW_Est_1to5 Bioversity International Inge Van den Bergh 1 hanging vertically Matooke smoothness in hand measurement scale 0-10 Elizabeth Kakhasa NARO, Uganda MatSmoothM_Meas_0to10 obtuse 4 Guillaume Bauchet FrtIntLng_M_cm IITA-BTI sparse blotching (<20%) 2 Bioversity International Inge Van den Bergh IntDisProp_Comp_pct without pigmentation 5 0 soft Guillaume Bauchet PulpDiam_M_cm IITA-BTI Rhiannon Crichton BnchWt_M_kg Bioversity International Guillaume Bauchet IITA-BTI PltEst_Date_ymd Rhiannon Crichton RchsWt_M_kg Bioversity International NmNumSlWt Danny Coyne IITA LfEmRate_Comp_LfMth Rhiannon Crichton Bioversity International Boiled plantain Sweetness scale 0-10 CIRAD / UNA, Côte d’Ivoire BoiPlantnSwt_Meas_0to10 Hermann Antonin KOUASSI 3 25-50 % of roots galled Inge Van den Bergh/Danny Coyne NmNumRtWt Bioversity International/IITA partially fused (up to 50 % of the length of the pedicel) 2 PratyCofNumRtWt2_Comp_Nema IITA Danny Coyne Bioversity International Rachel Chase AnthrCl_Est_1to16 Third or mature spot stage: the centres of the lesions dry out becoming light grey or buff coloured and a bright yellow transitional zone appears between them and the normal green colour of the leaf. The lesions remain clearly visible after the leaf has collapsed or withered because of their light coloured centre and dark border. 6 totLeafLength_Meas_cm KU Leuven lengthSum Sebastien Carpentier leafFreshMass_Meas_g KU Leuven leaf_fresh Sebastien Carpentier massFreshLeaf Rachel Chase PsdHght_E Bioversity International IITA-BTI Allan Brown/Guillaume Bauchet (temp.) FngSmshdPlpCl_Est_1to5 1 winged rootToPlantMassRatio_Comp_unitless Sebastien Carpentier massDryratioRoot relativeRoot_dry KU Leuven 8 pink-purple Bioversity International HvstDst_Comp_PlntHa Rhiannon Crichton 1 Initial speck stage: faint, minute, reddish-brown specks on the lower surface of the leaf. IITA WvlAdltMsrd_Ct_weevil Tendo Ssali Bioversity International Rachel Chase FrtLngth_M_cm 10 high intensity 67-100 % of all leaves turning yellow and necrotic, with leaves hanging down the pseudostem 4 7 pink-purple no symptoms 0 like extremely 5 PltNtHvstd_Ct_Plnt Rhiannon Crichton Bioversity International Bioversity International Rhiannon Crichton HandWt_M_kgPlnt Rhiannon Crichton FrtWt_M_gFruit Bioversity International BnchPst_Estm_1to5 Bioversity International Rachel Chase Rhiannon Crichton Bioversity International temp15 BrctClExtFc_Est_1to16 Bioversity International Rachel Chase IITA-BTI FngSmshdPlpSmth_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) Bioversity International MltHandWt_M_kgPlnt Rhiannon Crichton Tendo Ssali WvlAdltPrTrp_Ct_weevil IITA yellow 2 KU Leuven rwcPlant Sebastien Carpentier rwcPlant_Comp_unitless plant_rwc few flower relicts (< 20% of the fruits with relicts) 2 Sebastien Carpentier massDryRoot rootDryMass_Meas_g KU Leuven root_dry WvlAdltBdWght_M_mg IITA Tendo Ssali Danny Coyne IITA PratyNumSlWt_Comp_Nema Allan Brown Dwfsm_Est_1to2 IITA HandAvgWt_M_kgPlnt Rhiannon Crichton Bioversity International MatHardT_Meas_0to10 Elizabeth Kakhasa Matooke hardness in hand measurement scale 0-10 NARO, Uganda Bioversity Selection criteria for genotype that was ranked second lowest by female respondents during preference scoring exercise Noel Madalla Danny Coyne MeloiNumRtWt2_Comp_Nema IITA Rhiannon Crichton PStmHt_M1_cm Bioversity International Bioversity International Rhiannon Crichton FrtGrth_M_mm Tendo Ssali WvlAdltBdLgt_M_µM IITA NARO, Uganda MatMolT_Meas_0to10 Matooke moldability measurement scale 0-10 Elizabeth Kakhasa 2 First streak stage: the initial speck elongates, becoming slightly wider, to form a characteristic narrow, reddish brown streak up to 20 mm long and 2.0 mm wide, with the long axis parallel to the leaf venation. At this stage, streaks are more clearly visible on the lower surface of the leaf than on the upper one. The distribution of streaks is variable. Sometimes they are most numerous near the edge of the left side of the leaf. At other times they are equally numerous on both sides of the leaf, and more or less evenly distributed. Frequently they are densely aggregated in a band several cm wide on one side or both sides of the mid-rib, becoming less numerous towards the edge of the leaf. Streaks may be so numerous that every cm2 of leaf surface bears one or more. There is considerable variation in the length of individual streaks on a given leaf, and they frequently overlap to form larger, compound streaks. IITA Sckr_Est_1to2 Allan Brown slightly curved 2 2 intermeditae Bioversity International/IITA temp48 Inge Van den Bergh/Danny Coyne BnchShp_Estm_1to5 Bioversity International Rachel Chase DateDeath_Est_ymd Guillaume Bauchet IITA-BTI 4 curved (sharp curve) medium (20 cm < y < 30 cm) 2 PSuckN_Ct_PSuck Rhiannon Crichton Bioversity International Rhiannon Crichton Bioversity International BlkLfStrkSvrtLfNb_Ct_grd3 PMargWing_Est_1or5 Bioversity International Rachel Chase Selection criteria for genotype that was ranked lowest by male respondents during preference scoring exercise Noel Madalla Bioversity > 1/3 discoloured 5 purple brown 6 purple 5 6 Small bunch from neutral/hermaphrodite flowers just above the male bud SuckHtShtStg_M_cm Rhiannon Crichton Bioversity International MlBdShp_Comp_1to3 Bioversity International Rachel Chase other 10 Selection criteria for genotype that was ranked second highest by female respondents during preference scoring exercise Noel Madalla Bioversity Bioversity International temp21 Rhiannon Crichton Elizabeth Kakhasa NARO, Uganda MatSurfYellV_Meas_0to10 Matooke surface yellowness measurement scale 0-10 WvlLvPrTrp_Ct_weevil IITA Tendo Ssali YellowPres_Est_0or1 Bioversity International Inge Van den Bergh IITA WvlAdltHdCps_M_µM Tendo Ssali HelicoNumSlWt_Comp_Nema IITA Danny Coyne HoploNumRtWt2_Comp_Nema IITA Danny Coyne Bioversity Selection criteria for genotype that was ranked second highest by male respondents during preference scoring exercise Noel Madalla areaPlantTop area_topView topLeafArea_Meas_cm2 KU Leuven Sebastien Carpentier Christophe Jenny MlRchsTp_Estm_1to2 CIRAD IITA Tendo Ssali DmgIdxInn_M_% IITA Tendo Ssali temp44 Rhiannon Crichton Bioversity International BnchFrtNum_Comp_Frt other 9 Rhiannon Crichton OffTypEst_Date_dmy Bioversity International yellow 3 Bioversity International Rhiannon Crichton HvstDth_Comp_d IITA WvlLvBdWght_M_mg Tendo Ssali Matooke firmness in mouth measurement scale 0-10 NARO, Uganda Elizabeth Kakhasa MatFirmM_Meas_0to10 MatSour_Meas_0to10 Matooke sourness measurement scale 0-10 Elizabeth Kakhasa NARO, Uganda PMargClasp_Est_1or2 Bioversity International Rachel Chase IITA PratyCofNumSlWt_Comp_Nema Danny Coyne not dwarf 2 Bioversity International Rachel Chase BlotchA_Est_1to4 asymmetrical 3 HndRnk_Ct_HndRnk Rhiannon Crichton Bioversity International totLeafArea_Meas_cm2 KU Leuven areaTotLeaves areaLeaves Sebastien Carpentier Rhiannon Crichton Bioversity International BnchFrtNum_Ct_Frt Bioversity International Rachel Chase PMargCol_Esr_1to16 Bioversity International FrtExtLng_M_mm Rhiannon Crichton Noel Madalla Bioversity PA_EstFemale_Txt Rhiannon Crichton PltEst_Date_dmy Bioversity International Rhiannon Crichton Bioversity International BlkLfStrkSvrtLfNb_Ct_grd5 Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd0 Bioversity International Selection criteria for genotype that was ranked third lowest by male respondents during preference scoring exercise Noel Madalla Bioversity growthFresh_Comp_g plantGrowth_fresh KU Leuven Sebastien Carpentier growthFresh Bioversity International MgnBhvPtCnlThrdLf_Est_1to4 Rachel Chase Sebastien Carpentier widthSingleLeaf widthPerLeaf singleLeafWidth_Meas_cm KU Leuven Bioversity International BlkLfStrkLfFrStg6_Date_ymd Rhiannon Crichton Bioversity International Rachel Chase CmpdTplMnCl_Est_1to16 OdStdLfRk_Ct_leaf Rhiannon Crichton Bioversity International Bioversity International Rachel Chase FrtCvShp_Est_1to5 PltDnsty_Comp_PlntHa Bioversity International Rhiannon Crichton Rachel Chase PStmCol_Est_1to16 Bioversity International 26-30 cm 4 7 blue 11-30 egg masses 3 Bioversity International Rhiannon Crichton temp2 5 not winged and not clasping the pseudostem CIRAD / UNA, Côte d’Ivoire BoiPlantnMois_Meas_0to10 Boiled plantain Moistness scale 0-10 Hermann Antonin KOUASSI Noel Madalla Bioversity Selection criteria for genotype that was ranked highest by male respondents during preference scoring exercise Sebastien Carpentier waterLossPlant_Meas_ml waterLossPlant transpPlant KU Leuven FrtApxPt_Est_1to5 Bioversity International Rachel Chase Bioversity International Rachel Chase FrtNbMdHndBnch_Ct_ft 1 1-2 egg masses BoiPlantnChew_Meas_number of chews Hermann Antonin KOUASSI Boiled plantain Chewiness by number of chews CIRAD / UNA, Côte d’Ivoire AvgWvlLvBdWght_M_mg IITA Tendo Ssali Rhiannon Crichton Bioversity International DIT_Comp_d 0 Dry PratyNumRtWt2_Comp_Nema IITA Danny Coyne waterLossSystem_per_Area waterLossSystPerLeafArea_Meas_mlcm2 KU Leuven Sebastien Carpentier temp8 Rhiannon Crichton Bioversity International Inge Van den Bergh/Danny Coyne temp51 Bioversity International/IITA heterogeneous 0 Noel Madalla Selection criteria for genotype that was ranked third lowest by female respondents during preference scoring exercise Bioversity pseudostemDryMass_Meas_g massDryPseudostem KU Leuven ps_dry Sebastien Carpentier Bioversity International temp12 Rhiannon Crichton 1 bad Danny Coyne IITA PratyGooNumRtWt2_Comp_Nema FrtGrth_M_cm Guillaume Bauchet IITA-BTI 5 light green Rhiannon Crichton BnchHndNum_Ct_Hnd Bioversity International Noel Madalla Bioversity Selection criteria for genotype that was ranked lowest by female respondents during preference scoring exercise 10 dont know mole rats 8 Bioversity International temp22 Rhiannon Crichton entity Rhiannon Crichton Bioversity International temp19 RadoNumRtWt2_Comp_Nema Danny Coyne IITA Bioversity International Rachel Chase CmpdTplMnCl_Est_1to6 Guillaume Bauchet DateColl_Date_ymd IITA-BTI Guillaume Bauchet DateHarv_Date_ymd IITA-BTI FrtDiam_M_mm Rhiannon Crichton Bioversity International 3 Second-streak stage: the streaks may elongate slightly, but the most notable change is that in colour from reddish brown to dark brown or almost black, sometimes with a purplish tinge. The streak is now clearly visible on the upper surface of the leaf. The distribution of streaks at this stage of development varies in the same way as mentioned above. When streaks are very numerous, and more or less evenly distributed, the entire leaf blackens. 3 long (equal to or more than 30 cm) temp31 Rhiannon Crichton Bioversity International 2 winged and not clasping the pseudostem MltFrtWt_M_gFruit Rhiannon Crichton Bioversity International Noel Madalla Bioversity PS_CompResp_PrefScale 3 brown-black CmpdTplLbCl_Est_1to5 Bioversity International Rachel Chase Rhiannon Crichton AnCrpCyclPrp_Comp_CrpCclY Bioversity International First streak stage: specks elongate, becoming slightly wider to form narrow reddish-brown streaks. 2 no intensity 0 Rhiannon Crichton Bioversity International AvgFrtDiam_M_mm 4 like very much 1 green add** 1 green 1 Inge Van den Bergh/Danny Coyne Bioversity International/IITA temp50 truncated 1 VitCCnt_M_ Allan Brown/Guillaume Bauchet (temp.) IITA NARO, Uganda Matooke surface homogeneity of colour measurement scale 1-10 Elizabeth Kakhasa MatSurfHomColV_Meas_0to10 Allan Brown/Guillaume Bauchet (temp.) FngUnSmshdPlpTxt_Est_1to5 IITA-BTI PS_CompFemale_PrefScale Noel Madalla Bioversity Bioversity International FrtLngth_M_1to5 Rachel Chase Noel Madalla Bioversity PS_CompMale_PrefScale CmpdTplLbCl_Est_1to16 Bioversity International Rachel Chase PlntgDisTim_Comp_d Bioversity International Inge Van den Bergh Sebastien Carpentier leavesFormedPerPseudostemGrowth_Comp_percm KU Leuven leaves_per_heightPS discolouration of between one-third and two-thirds of vascular tissue 4 Guillaume Bauchet IITA-BTI AvgFrtIntLng_M_cm KU Leuven rateWaterLossPerDay Sebastien Carpentier waterLossDaily waterLossDay_Comp_ml 3 other (specify on answer sheet) Rhiannon Crichton Bioversity International BlkLfStrkLfFrStg6_Date_dmy 5 medium intensity Rhiannon Crichton temp13 Bioversity International Rhiannon Crichton temp11 Bioversity International massDryratioLeaf KU Leuven relativeLeaf_dry leafToPlantMassRatio_Comp_unitless Sebastien Carpentier KU Leuven waterLossSyst_Meas_ml transpSyst Sebastien Carpentier waterLossSystem FrtApxFlwRlt_Est_1to3 Bioversity International Rachel Chase Rhiannon Crichton PlntShtTime_Comp_d Bioversity International Bioversity International SSuckN_Ct_SSuck Rhiannon Crichton 3 < 1/3 discoloured PltDth_Comp_d Bioversity International Rhiannon Crichton KU Leuven rwcLeaf_Comp_unitless rwcLeaf leaf_rwc Sebastien Carpentier FlwT_Date_dmy Rhiannon Crichton Bioversity International 4 obtuse and split Elizabeth Kakhasa NARO, Uganda MatPump_Meas_2pt Matooke pumpkin aroma measurement 2pt scale IITA Tendo Ssali AvgWvlAdltHdCps_M_µM Bioversity International temp16 Rhiannon Crichton HndNumMsrd_Ct_Hnd Rhiannon Crichton Bioversity International rootShootRatioDry rootShootDry_Comp_unitless KU Leuven massDryRootShoot Sebastien Carpentier IITA TrpNum_Ct_weeviltrap Tendo Ssali pseudostemHeightIncrease KU Leuven Sebastien Carpentier pseudostemHeightIncrease_Comp_cm2 straight 1 0 absent IITA Tendo Ssali AvgWvlLvBdLgt_M_µM massFreshPseudostem pseudostem_fresh pseudostemFreshMass_Meas_g KU Leuven Sebastien Carpentier Inge Van den Bergh ExtDisProp_Comp_pct Bioversity International temp45 Bioversity International/IITA Inge Van den Bergh/Danny Coyne MatMoistM_Meas_0to10 Elizabeth Kakhasa NARO, Uganda Matooke moisture in mouth measurement scale 0-10 2 dont know Rhiannon Crichton Bioversity International Shoot_Date_dmy Allan Brown/Guillaume Bauchet (temp.) FngUnSmshdPlpSmth_Est_1to5 IITA-BTI Noel Madalla Bioversity PA_EstMale_Txt IITA Danny Coyne MeloiNumSlWt_Comp_Nema 6 Stage 6 is when the centre of the spot dries out, turns clear gray and is surrounded by a well-defined black ring, which is, in turn, surrounded by a bright yellow halo. These spots remain visible after the leaf has dried out because the ring persists. Rhiannon Crichton Bioversity International temp23 leafChlorosis_Comp_unitless KU Leuven leafChlorosis Sebastien Carpentier Rhiannon Crichton DateColl_Date_dmy Bioversity International PMargWing_Est_1or2 Bioversity International Rachel Chase 8 dark green not winged 2 Rhiannon Crichton BlkLfStrkLfFrStg1_Date_dmy Bioversity International Bioversity International Rachel Chase HndNbWhlBnch_Ct_hands IITA-Bioversity International Amos Alakonya-Rhiannon Crichton SptLfNb_Ct_leaf Rhiannon Crichton Bioversity International PulpDiam_M_mm Bioversity International BlkLfStrkLfFrStg1_Date_ymd Rhiannon Crichton Danny Coyne IITA PratyGooNumSlWt_Comp_Nema 4 50-75 % of roots galled Bioversity International SuckNum_Ct_Suck Rhiannon Crichton Inge Van den Bergh/Danny Coyne Bioversity International/IITA DeadRtNum2_Ct_Rt IITA Tendo Ssali AvgWvlAdltBdLgt_M_µM Bioversity International PtlClpFW_Comp_pct Rhiannon Crichton MgnBhvPtCnlThrdLf_Est_1to5 Bioversity International Rachel Chase IITA GdBnch_Est_1to2 Allan Brown Noel Madalla Bioversity Selection criteria for genotype that was ranked third highest by male respondents during preference scoring exercise 7 green highly imbricate 3 Rhiannon Crichton temp17 Bioversity International 5 Stage 5 is when the elliptical spot is totally black and has spread to the underside of the limb. It is surrounded by a yellow halo with the centre beginning to flatten out. 4 1/3-2/3 discoloured 3 persistent flower relicts (> 20% of the fruits with relicts) DateHarv_Date_dmy Rhiannon Crichton Bioversity International Bioversity International WSuckN_Ct_WSuck Rhiannon Crichton Rhiannon Crichton DthPrp_Comp_DdPltRt Bioversity International Bioversity International DeathCause_Est Rhiannon Crichton Inge Van den Bergh Bioversity International CormDiscol_Est_1to6 rwcRoot Sebastien Carpentier KU Leuven rwcRoot_Comp_unitless root_rwc totalNumLeaves numLeaf Sebastien Carpentier numLeaves_Count_unitless KU Leuven BrctBhvBfFll_Est_1to2 Bioversity International Rachel Chase pointed 1 1 less than or equal to 15 cm Bioversity International AvgPulpDiam_M_mm Rhiannon Crichton 8 other Bioversity International temp18 Rhiannon Crichton Bioversity International MlBdLngth_M_1to3 Rachel Chase temp14 Rhiannon Crichton Bioversity International Rhiannon Crichton temp20 Bioversity International LfChgPres_Est_1or2 Bioversity International Inge Van den Bergh Bioversity International Rachel Chase BlotchCol_Est_1to5 Bioversity International temp9 Rhiannon Crichton IITA-BTI Allan Brown/Guillaume Bauchet (temp.) FngUnSmshdPlpTst_Est_1to5 Sebastien Carpentier singleLeafArea_Meas_cm2 areaSingleLeaf areaPerLeaf KU Leuven Rachel Chase BrctApxShp_Est_1to5 Bioversity International Rachel Chase Bioversity International BlotchCol_Est_1to4 BlkLfStrkSvrtLfNb_Ct_grd2 Rhiannon Crichton Bioversity International 5 other Sebastien Carpentier plantFreshMass_Meas_g massFreshPlant KU Leuven plant_fresh 8 dark green BlkLfStrkSvrt_Est_Faure IITA-Bioversity International 2 neutral flowers on one to few hands only near the bunch (rest of stalk is bare) Rhiannon Crichton DET_Comp_d Bioversity International Tendo Ssali WvlLvMsrd_Ct_weevil IITA 13-16 2 1 dislike very much Rhiannon Crichton CauDam_Est_txt Bioversity International Bioversity International Rachel Chase FrtCvShp_Est_1to6 KU Leuven ps_rwc Sebastien Carpentier rwcPseudostem rwcPseudostem_Comp_unitless 1 present FlwT_Date_ymd Guillaume Bauchet IITA-BTI IITA Tendo Ssali WvlAdlt_Ct_weevil 3 Second-streak stage: streaks change colour from reddish brown to dark brown or black, sometimes with a purplish tinge, clearly visible at the upper surface of the leaf. Inge Van den Bergh/Danny Coyne Bioversity International/IITA temp46 1 Initial speck stage: symptoms are first visible to the naked eye as faint, minute (less than 0.25 mm diam), reddish brown specks on the lower surface of the leaf. Specks are often most abundant near the margin of the left side of the leaf, particularly towards the tip. Where leaf spot is severe, specks have been observed on the second leaf of plants that have not yet produced a bunch. Elsewhere they usually appear on the third, fourth, or older leaves. 5 bright yellow Allan Brown/Guillaume Bauchet (temp.) FngSmshdPlpArm_Est_1to5 IITA-BTI 2 straight in the distal part Bioversity International MlRchsPst_Estm_1to5 Rachel Chase soft 0 Inge Van den Bergh/Danny Coyne temp52 Bioversity International/IITA Rhiannon Crichton AvgFrtExtLng_M_cm Bioversity International 2 contrast between margin and petiole (with a contrasting colour line along) curved (sharp curve) 3 IITA Tendo Ssali DmgPrphl_M_% PetColpPres_Est_1or2 Inge Van den Bergh Bioversity International BlkLfStrkSvrt_Est_MedLawCpt IITA-Bioversity International 4 16-33% of lamina with symptoms IITA WvlLv_Ct_weevil Tendo Ssali green 2 Rhiannon Crichton FngWtTtl_Comp_kg Bioversity International weevils 6 IITA WvlLvHdCps_M_µM Tendo Ssali 5 margins overlapping FngSmshdPlpTst_Est_1to5 IITA-BTI Allan Brown/Guillaume Bauchet (temp.) Rhiannon Crichton Bioversity International CigLfUnrBrnStgB_Date_dmy 9 whitish totally fused (more than 50 % of the length of the pedicel) 3 Tendo Ssali AvgWvlAdltBdWght_M_mg IITA IITA-BTI Allan Brown/Guillaume Bauchet (temp.) FngUnSmshdPlpCl_Est_1to5 hard 10 BlkLfStrkSvrtLfNb_Ct_grd4 Rhiannon Crichton Bioversity International 1 cylindrical Inge Van den Bergh/Danny Coyne FuncRtNum2_Ct_Rt Bioversity International/IITA KU Leuven TEtotalDry Sebastien Carpentier transpirationEfficiency_Comp_gml TE_dry Hermann Antonin KOUASSI Boiled plantain Firmness scale 0-10 BoiPlantnFirm_Meas_0to10 CIRAD / UNA, Côte d’Ivoire > 75 % of roots galled 5 Noel Madalla Bioversity Selection criteria for genotype that was ranked third highest by female respondents during preference scoring exercise 2 < 25 % of roots galled Rhiannon Crichton RtnCrpCyc_Comp_d Bioversity International fat (w/y equal to or more than 0.55) 3 1 like a top 1 less than or equal to 20 cm 3 straight with erect margins Rhiannon Crichton LfEmRate_Comp_LfWk Bioversity International 2 isolated points of discolouration in vascular tissue orange 6 4 green yellow adult female 2 Bioversity International Rachel Chase FrtftPdclLgth_Est_1to3 2 lanceolate no contrast between margin and petiole (without a colour line along) 1 male flowers/bracts above the male bud (rest of stalk is bare) 3 BlkLfStrkInfIndex_Comp_Idx Bioversity International Rhiannon Crichton Noel Madalla Bioversity Selection criteria for genotype that was ranked second lowest by male respondents during preference scoring exercise Rhiannon Crichton CropCyc_Comp_d Bioversity International orange red 10 7 Other 3 watery green Tendo Ssali WvlLvBdLgt_M_µM IITA Rhiannon Crichton Bioversity International PltPltd_Ct_Plnt Rhiannon Crichton SptPsdStmPercFW_Comp_pct Bioversity International 2 brown banana bunchy top (BBTD) 4 Rhiannon Crichton HndFrtNum_Ct_Frt Bioversity International few internal spots 2 1 skinny (w/y less than or equal to 0.45) Rachel Chase AnthrCl_Est_1to8 Bioversity International PA_EstResp_Txt Noel Madalla Bioversity temp10 Bioversity International Rhiannon Crichton 1 yellow KU Leuven Sebastien Carpentier totalNumFunctionalLeaves numFuncLeaves_Count_unitless numFuncLeaf Rhiannon Crichton temp30 Bioversity International 3 intermediate 4 neutral/male flowers and presence of withered bracts on the entire stalk 3 34-66 % of older leaves turning yellow, with some hanging down the pseudostem sticky 10 purple 6 1 bare 3 purple to blue Stage 1 appears as a small depigmentation spot whose whitish or yellow colour resembles stage 1 of Yellow Sigatoka disease. These symptoms are not visible in transmitted light and can be observed only on the underside of the leaf. 1 1 orange-brown (mahogany, like in Pisang Mas) leafDryMass_Meas_g Sebastien Carpentier massDryLeaf leaf_dry KU Leuven Rhiannon Crichton Bioversity International PltFstLfSptd_Ct_leaf Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd6 Bioversity International temp47 Bioversity International/IITA Inge Van den Bergh/Danny Coyne 3 large blotches white 1 1 parthenocarpic wide with erect margins 2 Selection criteria for genotype that was ranked highest by female respondents during preference scoring exercise Noel Madalla Bioversity temp41 Bioversity International Rhiannon Crichton Rhiannon Crichton AvgPeelThck_M_mm Bioversity International DamEst_Date_ymd Guillaume Bauchet IITA-BTI Banana ontology Plantain ontology Bioversity International Inge Van den Bergh PStmDiscol_Est_cm 2 cream 1 revolute (rolling) temp38 Stellenbosch University Altus Viljoen black-purple 3 5 medium intensity no intensity 0 Amos Alakonya-Rhiannon Crichton IITA-Bioversity International StdLf_Ct_leaf Rhiannon Crichton Bioversity International AvgFrtGrth_M_mm Allan Brown IITA GdFrtFll_Est_1to2 no pigmentation 1 Rhiannon Crichton HvstPrp_Comp_PlntNb Bioversity International Other 5 KU Leuven areaPlant_per_areaTot Sebastien Carpentier leafAreaRatio_Comp_unitless areaRatio present 2 BrctClExtFc_Int_1to8 Bioversity International Rachel Chase moderate blotching (20-50%) 3 less than or equal to 2 m 1 sparse blotching 1 RkPvsMktLf_Comp_leafrank Rhiannon Crichton Bioversity International Rhiannon Crichton PltSpcg_Comp_m2 Bioversity International AcAnYd_Comp_TnHaY Rhiannon Crichton Bioversity International black-purple 4 Bioversity International PltDead_Ct_Plnt Rhiannon Crichton Sebastien Carpentier heightPS heightPseudostem lengthPseudostem_Meas_cm KU Leuven 6 other 1 absent Bioversity International Rachel Chase FstBrctApxShp_Est_1to4 Bioversity International PMargColL_Est_1or2 Rachel Chase 5 erect lengthSingleLeaf lengthPerLeaf KU Leuven singleLeafLength_Meas_cm Sebastien Carpentier 1 High shouldered (x/y less than or equal to 0.28) 6 Third or mature spot stage: the centre of the spot dries out, becoming light grey or buff-coloured, and further depressed. The spot is surrounded by a narrow, well-defined, dark brown or black border. between the latter and the normal green colour of the leaf, there is often a bright yellow transitional zone. After the leaf has collapsed and withered, spots remain clearly visible because of the light-coloured centre and dark border. at an angle 2 PStmSplitPres_Est_1or2 Bioversity International Inge Van den Bergh other 4 temp49 Inge Van den Bergh/Danny Coyne Bioversity International/IITA IITA-BTI FngSmshdPlpTxt_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) banana Xanthomonas wilt (BXW) 3 IITA Tendo Ssali AvgWvlLvHdCps_M_µM Bioversity International FstExtDiSmptFW_Date_ymd Rhiannon Crichton MlBdLngthRt_M_1to3 Bioversity International Rachel Chase growthPS_per_leafFormed KU Leuven pseudostemHeightIncreasePerFormedLeaf_Comp_cm Sebastien Carpentier Rachel Chase Bioversity International FrtftPdclLgth_M_mm slightly pointed 2 YellowPres_Est_1or2 Bioversity International Inge Van den Bergh temp43 IITA Tendo Ssali growthDry_Comp_g KU Leuven Sebastien Carpentier plantGrowth_dry growthDry root_fresh KU Leuven massFreshRoot Sebastien Carpentier rootFreshMass_Meas_g white 1 Second-spot stage: the dark brown or black central area of the spot becomes slightly depressed and the water-soaked border becomes more pronounced due to darkening. At this stage, a slight yellowing of the leaf tissue immediately surrounding the water-soaked border may occur. 5 NARO, Uganda Matooke sweetness measurement scale 0-10 MatSweet_Meas_0to10 Elizabeth Kakhasa red-purple 4 FrtNb_Ct_gFruit Rhiannon Crichton Bioversity International homogeneous 10 rounded 5 3 blunt-tipped (plateau at tip) Bioversity International Rachel Chase MlRchsApp_Estm_1to7 Rhiannon Crichton Bioversity International AnPltPrp_Comp_KgHaY Bioversity International Rachel Chase PdcFsn_Est_1to2 OffTypEst_Date_ymd Guillaume Bauchet IITA-BTI nematodes 7 MatStickT_Meas_0to10 NARO, Uganda Elizabeth Kakhasa Matooke stickiness in hand measurement scale 0-10 open with margins spreading 1 3 with a curve 3 Stage 3 differs from the previous one by its dimensions. The stripe becomes longer, is enlarged and in certain conditions (weak inoculum and unfavourable climatic conditions) can reach 2 or 3 cm. Rachel Chase Bioversity International BrctClExtFc_Int_1to16 whitish 9 RadoNumSlWt_Comp_Nema IITA Danny Coyne Bioversity International FrtApxFlwRlt_Est_1to4 Rachel Chase 10 high intensity CigLfPigm_Est_1to3 Rachel Chase Bioversity International short (y less than or equal to 20 cm) 1 Bioversity International FrtExtLng_M_cm Rhiannon Crichton IITA-BTI BnchMatStg_Est_1to2 Guillaume Bauchet 11-20 mm 2 KU Leuven relativePS_dry Sebastien Carpentier pseudostemToPlantMassRatio_Comp_unitless massDryratioPseudostem Boiled plantain Stickiness scale 0-10 CIRAD / UNA, Côte d’Ivoire BoiPlantnStic_Meas_0to10 Hermann Antonin KOUASSI Rhiannon Crichton NtHvstDst_Comp_PlntHa Bioversity International 4 spiral (all fruit are attached to a unique crown coiled around the stalk) strongly bottle-necked (wider under tip than number 2) 4 1 leaf spots 2 16-20 cm 4 horizontal or supra-horizontal Bioversity International DamEst_Date_dmy Rhiannon Crichton 2 cream medium intensity 5 10 orange red PetColpPres_Est_0or1 Bioversity International Inge Van den Bergh 1 less than or equal to 12 Rachel Chase BrctImbrcApxMlBd_Est_1to3 Bioversity International Allan Brown IITA PdltFrt_Est_1to2 orange-red 9 Inge Van den Bergh LfChgPres_Est_0or1 Bioversity International 34-50% of lamina with symptoms 5 yellow 2 curved in slight S shape (double curvature) 5 Rhiannon Crichton Bioversity International NtHvstPrp_Comp_PlntNb purple-brown 5 Bioversity International Rachel Chase BrctClExtFc_Est_1to10 3 red IITA Tendo Ssali DmgIdxOut_M_% present 2 Bioversity International Rachel Chase MlBdShd_Comp_1to3 Rhiannon Crichton ChgNwLfPercFW_Comp_pct Bioversity International margins curved inward 4 NSpotLfProp_Comp_idx IITA-Bioversity International moderately imbricate 2 grainy 5 temp53 Inge Van den Bergh/Danny Coyne Bioversity International/IITA PStmHt_M2_cm Rhiannon Crichton Bioversity International Inge Van den Bergh CrmDiscDistTlstSckFW_M_cm Bioversity International 5 firm 2 dark brown Bioversity International FstYngLfsptd_Est_ymd Rhiannon Crichton without flower relicts 1 PltHvstd_Ct_Plnt Rhiannon Crichton Bioversity International 1 pointed not parthenocarpic 2 Rhiannon Crichton Bioversity International temp32 3 like fairly Rhiannon Crichton Bioversity International PeelThck_M_mm absent 0 4 First-spot stage: the streaks broaden and become more or less fusiform or elliptical in outline and a water soaked border appears around each lesion. not clasping 2 HoploNumSlWt_Comp_Nema Danny Coyne IITA Bioversity International Rhiannon Crichton HndFrtAvgNum_Ct_Frt CIRAD / UNA, Côte d’Ivoire BoiPlantnMeal_Meas_0to10 Hermann Antonin KOUASSI Boiled plantain Mealiness scale 0-10 Bioversity International MSuckN_Ct_MSuck Rhiannon Crichton Sebastien Carpentier massDryPlant plantDryMass_Meas_g KU Leuven plant_dry acronym crumbly 0 3 equal to or more than 3 m dwarf 1 Rhiannon Crichton Bioversity International ShtHvstTime_Comp_d 4 Stage 4 appears on the underside as a brown spot and on the upper side as a black spot. 6 51-100% of lamina with symptoms 3 straight in the distal part Rhiannon Crichton Bioversity International FrtWtAvg_M_g Rhiannon Crichton Bioversity International temp40 0 lumpy 3-10 egg masses 2 Stage 2 appears as a stripe, generally brown in colour and visible on the underside of the leaf, later the symptom also appears on the upper part of the limb as a stripe, the yellow colour of which resembles the stripe at stage 1 of Yellow Sigatoka. The colour of this stripe will change progressively from brown and later to black on the upper side of the limb, but will retain the brown colour on the underside. 2 1 corm completely clean, no vascular discolouration 1 trace infections with a few small galls KU Leuven leafAreaIncrease_Comp_cm2 leafAreaIncrease Sebastien Carpentier Rhiannon Crichton DthDnst_Comp_PlntHa Bioversity International egg 4 temp37 Stellenbosch University Altus Viljoen Bioversity International Rachel Chase PMargCol_Esr_1to4 3 discolouration of up to one-third of vascular tissue watery green 3 8 other YlLfPercFW_Comp_pct Rhiannon Crichton Bioversity International 7 orange red red-purple 2 4 horizontal Rhiannon Crichton CigLfUnrBrnStgB_Date_ymd Bioversity International adult male 1 5 equal to or more than 31 cm 1-5% of lamina with symptoms 2 red 8 Guillaume Bauchet IITA-BTI Shoot_Date_ymd not revolute (not rolling) 2 Matooke grassy aroma measurement 2pt scale Elizabeth Kakhasa NARO, Uganda MatGrass_Meas_2pt absent 0 good 2 Matooke astringency measurement scale 0-10 Elizabeth Kakhasa NARO, Uganda MatAstr_Meas_0to10 Matooke aroma measurement scale 0-10 MatAro_Meas_0to10 Elizabeth Kakhasa NARO, Uganda 4 green yellow 6 medium green 1 winged and undulating 7 black (anthers aborted) IITA temp42 Tendo Ssali 2 21-30 cm Danny Coyne IITA RotyNumSlWt_CompNema 3 juvenile slightly angled 2 2 margins erect 3 equal to or more than 21 mm 3 21-25 cm light green 5 1 no visible sign of fusion 9 red-purple 3 6-15% of lamina with symptoms 1 cream 4 First-spot stage: the streak broadens and becomes more or less fusiform and elliptical in outline and similar to the first-spot stage of Sigatoka (Leach 1946). The transition from streak to spot is further characterised by the development of a light brown, water-soaked border around the spot. This water-soaked effect is especially clear in the early morning, when dew is still present on the leaf, or after rain. moldable 10 plant dead, with brown leaves hanging down the pseudostem 5 10 high intensity straight (or slightly curved) 1 Bioversity International Rachel Chase MlBdShp_Comp_1to5 5 erect > 100 egg masses 5 4 curved in S shape (double curvature) 10 bright yellow clasping 1 4 31-100 egg masses 2 pink-purple to red 2 0-33 % of older banana leaves turning yellow 1 pointed 10 firm 2 ** persistent style 2 yellow 4 extensive pigmentation (>50%) 4 0 very light yellow 3 low shouldered (x/y more than or equal to 0.30) 3 hanging at a 45 degree angle less than or equal to 10 mm 1 Bioversity International Inge Van den Bergh PStmSplitPres_Est_0or1 other 5 no visual leaf symptoms 1 convolute 1 Moist 10 BlkLfStrkSvrt_Est_MedLawCdsd IITA-Bioversity International 2 medium shouldered (0.28 < x/y < 0.30) medium (0.45 < w/y < 0.55) 2 4 other Amos Alakonya-Rhiannon Crichton IITA-Bioversity International FstYngLfsptd_Est_dmy non sticky 0 1 margins spreading 10 smooth pink/pink-purple 10 attribute 6 medium green Bioversity International BlotchA_Est_1to5 Rachel Chase 3 obtuse high intensity 10 present 1 falling vertically 1 equal to or more than 17 3 other 5 ivory 3 2 2.1-2.9 m 0 no egg masses cream 1 6 entire inner rhizome discoloured 5 rounded without any floral relicts 1 2 dislike FlwHvstTime_Comp_d Rhiannon Crichton Bioversity International 4 extensive pigmentation intermediate 3 4 only base of the style persists 3 equal to or more than 31 cm 5 brown/rusty brown 5 medium intensity 0 low intensity fusarium wilt 2 no internal symptoms 1 margins curved inward 3 total discolouration of vascular tissue 6 winged and clasping the pseudostem 3 neutral/male flowers on the whole stalk without persistent bracts (still firmly attached to the rachis) 5 2 lengthily pointed (like plantain) 7 green base of the style prominent 3 2 truncate ( fully green 1 4 ovoid 5 Second-spot stage: the dark brown or black centre of each lesion becomes slightly depressed and water soaked border becomes more pronounced. 1 less than 1% of lamina with symptoms, only streaks and/or up to 10 spots not winged and clasping the pseudostem 4 small blotches 2 brown 1 2 one yellow finger Male bud size - measurement Length and maximum diamter of male bud at harvest. Colour of pigmentation on outer surface of cigar leaf on a developed sucker Pigmentation of outer surface Pigmentation of outer surface of cigar leaf on sucker Youngest leaf spotted - counting Record the rank (order) of the youngest leaf spotted (the first fully unfurled leaf with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres), counting the rank by starting with the youngest completely unrolled leaf as 1 and moving downwards, associating the data with the Date of data collection. Colour of bract internal face - estimation Visually observe the colour of the internal face of the first unlifted bract. Use colour chart A and observe out of direct sunlight. blotch color scale (1 to 4) Fruit smashed pulp colour The color of the smashed pulp of a mature fruit Colour Number of leaves with a black leaf streak disease severity grade of 2 The number of leaves on a plant with a black leaf streak disease severity grade of 2. Number of leaves with black leaf streak disease severity grade 2 pendulantfruit The overall estimation fruit pendulance on a bunch Overall fruit pendulance quality Finger pendulance quality µM bunch position scale(1 to 5) Remains of flower relicts at fruit apex Remains of flower relicts at apex Percentage of flower relicts at fruit apex Number of hands Number of hands on whole bunch Number of hands on bunch **subsume Root necrosis caused by nematodes - estimation Select at random five functional primary roots, at least 10 cm long. Reduce the length of the five selected roots to 10 cm and slice through the roots length-wise. Evaluate one half of each of the five roots for the percentage of root cortex showing necrosis. The maximum root necrosis per root half can be 20 %, giving a maximum root necrosis of 100 % for the five halves together. Mentally determine the necrosis of the individual roots (1 to 5) and sum these together to get the total root necrosis of the sample. Egg-laying female root knot nematode Put a part of the sample between thumb and index fingers and using tapping motions, evaluate the amount of product adhering on them Stickiness in hand method Ratoon crop cycle - computation Calculate as: the time elapsed between the Date of harvest of the bunch of the plant from the preceeding crop cycle and the Date of harvest of the bunch of the plant from the current crop cycle, e.g. 24/11/2015 - 24/12/2014 = 335 days. multiplication factor Ratio of pseudostem dry mass versus plant dry mass Ratio - computation Time elapsed from planting to first youngest leaf spotted Time elapsed between planting and the appearance of the first youngest leaf spotted. Time from planting to first youngest leaf spotted The average daily water loss Rate waterLossDay Daily rate of water loss rachis type scale (1 to 2) leafAreaRatio Ratio of projected leaf area to the actual leaf area, measure for the compactness of the plant crown Ratio Leaf area to plant area ratio cm? Population density Number of Pratylenchus goodeyi per fresh root weight Number of Pratylenchus goodeyi in roots Focus group discussion for lowest ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked lowest in preference ranking exercise Measure the distance from the collar, or from the pseudostem base at the ground if the collar is not visible, to the intersection of the petioles of the two youngest leaves (leaf ranks 1 and 2), using a measuring pole or sliding ruler. Plant height - petiole of two youngest leaves - measurement g / mL Calculate as: the time elapsed between the Date of planting and the Date of first youngest leaf spotted, e.g. 24/06/2014 - 24/01/2014 = 151 days. Time from planting to first youngest spotted leaf - computation Presence of splitting pseudostem base caused by Fusarium wilt - estimation Visual assessment. Calculate as: the sum of the number of values for Date of harvest. Number of plants harvested - computation cm Water content - computation (Plant Fresh Weight - Plant Dry Weight) / Plant Fresh Weight Distance of discolouration Distance of internal discolouration in pseudostem of plant caused by Fusarium wilt The distance of discolouration in the pseudostem of the plant, caused by Fusarium wilt. Boiled plantain Boiled plantain Firmness Force required to obtain deformation, penetration or rupture of the banana Firmness BoiPlantnFirm Black leaf streak disease severity index - computation Calculate as: ((((Number of leaves with black leaf streak disease severity grade 0, multiplied by 0), plus (Number of leaves with black leaf streak disease severity grade 1, multiplied by 1), plus (Number of leaves with black leaf streak disease severity grade 2, multiplied by 2), plus (Number of leaves with black leaf streak disease severity grade 3, multiplied by 3), plus (Number of leaves with black leaf streak disease severity grade 4, multiplied by 4), plus (Number of leaves with black leaf streak disease severity grade 5, multiplied by 5), plus (Number of leaves with black leaf streak disease severity grade 6, multiplied by 6)), divided by the number of grades in the scale minus 1 (i.e. 7-1)), divided by the Number of standing leaves), multiplied by 100. For example, ((((3 * 0) + (1 * 1) + (1 * 2) + (3 * 3) + (5 * 4) + (0 * 5) + (0 * 6)) / 6) / 13) * 100 = ((32 / 6) / 13) * 100 = 41. g Fresh weight plantFreshMass Plant fresh weight A plant weight trait which is the fresh weight of a plant Plant circumference at collar - measurement Measure the circumference of the pseudostem of the plant at the collar, or from the pseudostem base at the ground if the collar is not visible. BD colour chart A - 2016 Smoothness-Mouth feel - estimation Mouth feel assessment of smashed and unsmashed fruit pulp MatAroGrassA Matooke aroma grassy Grassy-like aroma Aroma of fresh grass Matooke Ratio - computation Ratio of root dry mass versus plant dry mass Multiple finger weight multfingerweight Weight The weight of multiple fingers. Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Use colour chart A and observe out of direct sunlight. Record the colour of the margin (general colour is below the rim). [x Observe at flowering time.] Petiole margin colour - estimation Record a description of the damage to the plant, e.g. leaves ripped, pseudostem snapped, plant toppled, etc. Damage description - estimation Angle to the vertical Bunch position Angle between the axis of the bunch and the vertical Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Pratylenchus goodeyi) per unit fresh root weight -method Extent of internal discolouration in corm of plant caused by Fusarium wilt - estimation The extent to which vascular discolouration extends up the pseudostem should be determined by making cross-section cuts, from the base of the pseudostem upwards, and examining the internal tissues following each cut. The point at which discolouration is no longer visible should be noted and the distance from this point to the pseudostem base recorded. Focus group discussion for second lowest ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was second ranked lowest in preference ranking exercise Main colour of the first external unlifted bract external face Colour of the external face Colour of bract external face Proportion of crop cycle that takes place in one year anncropcycprop The proportion of the crop cycle that takes place in one year. Annual crop cycle proportion Number of leaves with a black leaf streak disease severity grade of 5 The number of leaves on a plant with a black leaf streak disease severity grade of 5. Number of leaves with black leaf streak disease severity grade 5 The average length of a weevil larvae body, from the head to the tip of the abdomen. Banana weevil larvae average body length Average body length Ratio of leaf dry mass versus plant dry mass Ratio - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 1. Number of leaves with black leaf streak disease severity grade 1 - computation parthenocarpy Average potential annual yield Average potential annual yield The average potential annual yield, assuming that all plants are harvested, from the number of plants planted. potannyield Male rachis type Behaviour of male rachis - truncated or present. Truncated means there is no bract scar below the last hand. Present means there is a degenerated or persistent male bud Presence Computation of top leaf area with total (loose) leaf area Leaf area ratio - computation The rank (order) of the oldest (most-distal) standing leaf (with an erect petiole). Rank of oldest standing leaf Rank of oldest standing leaf weevil Matooke MatAstr complex sensation, accompanied by shrinking, drawing or puckering of the skin or mucosal surface in the mouth, produced by substances such as kaki tannins or sloe tannins Astringency Matooke astringency Shape Shape of the fully developed bunch on a plant Bunch shape Number of fingers in hand - counting Count how many fingers are in a hand. Associate the data with the Hand rank. The recommendation is to collect this data from the second and second-most distal hands. Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked second highest in preference ranking exercise Focus group discussion for second ranked traits with male respondents Rating of internal symptoms of Fusarium wilt in the greenhouse Discolouration caused by Fusarium wilt rating A rating of the extent of rhizome (corm) discolouration, caused by Fusarium wilt. Calculate as: the sum of the number of plants with any or multiple of the internal disease symptoms caused by Fusarium wilt - discolouration in the corm, and/or discolouration in the pseudostem - divided by the Number of plants planted * 100, e.g. 108 / 300 * 100 = 36%. Percentage of plants with internal disease symptoms caused by Fusarium wilt - computation day The number of plants that are planted, in a defined area. Plant density Combined density of plants planted plantdens Cut a transverse cross section of the corm at 10 cm below the collar (lower cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Damage to lower outer corm weevils - measurement Percentage of plants with changes in new leaves caused by Fusarium wilt Percentage of plants with changes in new leaves, caused by Fusarium wilt. Combined percentage with changes in new leaves Selection traits (text) Rachis weight - computation Calculate as: Bunch weight minus the sum of the Hand weight measurements coming from the count of number of hands (equivalent to Number of hands in bunch), e.g. 50 - (2.4 + 3.6 + 3.2 + 4.1 + 4.0 + 3.9 + 3.6 + 3.3 + 3.3 + 2.9 + 2.9 + 2.8) = 10.0. Calculate as: the Number of dead roots divided by the Number of all roots, multiplied by 100, e.g. 8 / 20 * 100 = 40 % dead roots. Percentage of dead roots - computation nematodes/unit of fresh root weight The circumference of the pseudostem of the plant at 20 cm from the collar. Plant circumference 20 cm from the collar Circumference 20 cm above the ground plantcircumf20cm Appearance in relation to the presence or absence of neutral, male and/or hermaphrodite flowers Male rachis appearance Appearance of flowers/bracts on the male rachis 80?C, for 14 days, or longer untill dry Dry weight - measurement Presence of splitting pseudostem base caused by Fusarium wilt - estimation Visual assessment. The average of how many banana weevil larvae caught per trap. Average number of banana weevil larvae Average number of banana weevil larvae per trap pseudostemFreshMass Fresh weight A pseudostem weight trait which is the fresh weight of a pseudostem Pseudostem fresh weight Distance of internal discolouration in pseudostem of plant caused by Fusarium wilt For the plant, the complete corm should be removed from the soil, the roots cut off and excess soil removed. Cross-sections of the corm should be cut (using a guillotine or other suitable device) to obtain five slices of equal thickness. The upper surface of each cut section should be examined and visually evaluate the extent of the vascular discolouration. BD colour chart A - 2016 Circumference 75 cm above the ground plantcircumf75cm Plant circumference 75 cm from the collar The circumference of the pseudostem of the plant at 75 cm from the collar. nematode lifecycle stages Count how many of all types of suckers are in the mat. Number of suckers - counting The nematode species found in the banana plant root sample. Species present Nematode species Multiple number of fingers in hand - counting Count how many fingers are in multiple hands. Associate the data with the Hand rank and the Number of hands measured. The recommendation is to collect this data from the second and second-most distal hands. (Root Fresh Weight - Root Dry Weight) / Root Fresh Weight Water content - computation Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 and the Date of tagged cigar leaf unrolling at stage B, e.g. 24/06/2014 - 24/03/2014 = 92 days. Black leaf streak disease development time - computation Height Tallest sucker height suckerheight The height of the pseudostem of the tallest sucker within the same mat as the plant (the sucker that will become the next plant with a bunch, once the bunch from the current plant is harvested) How many banana weevil adults used to obtain a measurement. Number of adult weevils measured Number of banana weevil adults measured unitless BD colour chart B - 1996 MatSour Matooke sourness Sourness Matooke gustatory complex sensation, generally due to presence of organic acids Lifecycle stages present Nematode lifecycle stage The lifecycle stage(s) of the nematode species found in the banana plant root sample. Nematode multiplication from single root inoculation of 50 nematodes per 8 cm length after 12 weeks Population density Nematode reproduction factor (microplot screening) Taste The taste of the unsmashed pulp of a mature fruit Fruit unsmashed pulp taste Weighing of water loss though system between timepoints (refer to experimental metadata) Water loss - measurement Presence absence 0 None Index of black leaf streak disease severity waterLossSyst The amount of water loss in the system over time Volume System water loss The rank (position) of the youngest completely unrolled leaf. Rank of marked youngest leaf Rank rankyoungleaf Potential annual yield - computation Calculate as: the Bunch weight divided by 1,000, multiplied by the Annual crop cycle proportion, multiplied by the Plant density, e.g. (50 / 1,000) * 1.09 * 1,667 = 90.85. Tallest sucker number of functional leaves - counting Count how many functional leaves (leaves that have 50% or more of their surface as green, healthy, photosynthetic tissue) are on the tallest sucker. Firmness scale 0-10 % Overall plant appearance PS Preference score Calculation of preference score by respondents Matooke-like aroma Aroma of the local matooke. Matooke aroma matooke Matooke MatAroMatA Description of how off-type describeofftype Off-type description A description of what made the plant recogniseable as an off-type. Percentage of plants with changes in new leaves caused by Fusarium wilt - computation Calculate as: the sum of the number of values for Presence of changes in new leaves caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 67 / 300 * 100 = 22%. Weekly leaf emission rate - computation Calculate as: the Rank of previously marked leaf at one point in time, minus 1 (equivalent to the Rank of marked youngest leaf), divided by the time elapsed between the two Date of data collection events when the marked leaf was recorded as 1) the Rank of marked youngest leaf and 2) the Rank of previously marked leaf, e.g. (2 - 1) / 1 week = LER of 1 leaves/week during a particular time period. 80?C, for 14 days, or longer untill dry Dry weight - measurement Head capsule width Banana weevil larvae head capsule width The width of a weevil larvae head capsule. BD colour chart A - 1996 Visual estimation of a fruit fill, (or parthenocarpic) for Early Evaluation Trial stage only. Fruit parthenocarpy - estimation Pigmentation of outer surface of cigar leaf - estimation Visually observe the pigmentation of the outer surface of the cigar leaf of a developed sucker or on another plant of the same accession. [x Observation should be made before shooting, on a developed sucker or on another plant of the same accession.] Dwarfism of plants The presence or absence of dwarfism on a mat Dwarfism dwarfism The time elapsed from planting to shooting (when the inflorescence emerges from the pseudostem and is still in an erect position). plant2shoot Time from planting to shooting Time from planting to shooting water suckers Presence absence 1 Main colour of the face of anther opposite to dehiscence Colour Anther colour Water loss rate - computation Measurement of system water loss between two timepoints (refer to experimental metadata) Calculate as: the sum of values with a Black leaf streak disease severity grade of 6. Number of leaves with black leaf streak disease severity grade 6 - computation Population density Nematode population density by species in soil Number of nematodes in soil Dry plant growth Dry mass accumulation over time Growth growthDry Number of fingers in bunch - counting Count how many fingers are in the bunch. Weight The weight of the bunch, including the rachis. bunchweight Bunch weight Genotype selection traits Description of traits considered important in selection of second least genotype by respondents Selection criteria for genotype ranked second lowest by respondents Record the relative surface area coverage by blotches. [x Look at several plants if possible to get an overall idea. Observe at flowering time]. Blotches area - estimation Date of death datedeath The date of death of the plant. Date of death open answer Anther colour - estimation The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the anther colour on the face opposite to the dehiscence split of the anther. Use colour chart B and observe out of direct sunlight. Date of shooting The date the inflorescence emerges from the pseudostem and is still in an erect position. Date of shooting dateshoot Main colour of the tip of the compound tepal (lobe) Lobe colour at tip of the tepal Lobe colour of tip of compound tepal Leaf width - measurement The shortest diagonal of a fitted ellips Measurement under microscope Weevil larvae body length - measurement Date of weevil damage to corm assessment - estimation Record the date of event. Number of nematodes (Pratylenchus coffeae) per unit fresh root weight - method Count from 100 ml of soil sample Leaf dry weight The dry weight of a leaf Dry weight leafDryMass Nematode stage - estimation Identify the nematode stage, using the options. open answer Angle of the part of the rachis between the last hand and the male bud Male rachis position Angle of the rachis between last hand and male bud The date the last bracts fall from the most distal hand (closest to the male bud) to display the female flowers. Date of flowering dateflower Date of flowering Count how many fingers are in the second-most distal hand. Number of fingers in 2nd-most distal hand - counting Matooke Moldability in hand MatMolT Matooke moldability mechanical textural attribute relating to the degree to which a substance can be deformed before it breaks harvest2death The time elapsed from the harvest of the bunch of the plant from the preceding crop cycle to the death of the plant from the current crop cycle. Time from harvest to death Time from harvest to death Calculate as: the Bunch weight, minus the Rachis weight, e.g. 50 - 10 = 40. Total finger weight - computation Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 The date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6. Date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6 fruit apex scale (1 to 5) Combined density of plants harvested harvdens Harvest density The number of plants that are harvested, in a defined area. Colour line along edge of petiole margin - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Record on the last developed leaf at [x flowering stage] [shooting]. Number of adult weevil trap - counting Visual counting method of Weevil traps (the trap should be minimum 30 cm long). datestarttimedatacoll The date and time of the start of data collection on a plant. Start date and time of data collection Date and time of start of data collection plantcircumfcollar The circumference of the pseudostem of the plant at the collar. Circumference at collar Plant circumference at collar Behaviour before falling, whether revolute or not revolute Bract behaviour before falling Behaviour of the last lifted bract - revolute or not revolute bract apex scale (1 to 4) Preference number scale handid hand rank The rank (order) of a hand in the bunch. Rank Hand rank The extent of discolouration in the corm of the tallest sucker, caused by Fusarium wilt. Extent of discolouration Extent of internal discolouration in corm of tallest sucker caused by Fusarium wilt Percentage of functional roots Percentage of functional roots The percentage of all roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant that are functional. A root weight trait which is the fresh weight of a root Root fresh weight rootFreshMass Fresh weight A rate that expresses the number of leaves that have emerged from the pseudostem in a week. Weekly leaf emission rate LER Weekly leaf emission rate Bunch shape - estimation Visually observe the shape of the mature bunch on a fully developed plant that is not experiencing environmental stress and choose the option that is most similar, from the 4 photos of descriptor 6.4.7. in the reference material. Blotches relative area at petiole base Percentage coverage of petiole base by blotches. Blotch surface area Date of tagged cigar leaf unrolling at Bruns stage B - estimation Record the date of event. Cut a finger in half, crosswise at its widest point, and on the cross-section measure the width of the peel, using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger peel thickness - measurement Number of fingers Number of fingers in 2nd hand The number of fingers in the second hand. numfingers2ndhand When you receive the sample, observe the surface and evaluate the homogeneity Surface color homogeneity Appearance method Number of Meloidogyne spp. in soil Population density Number of Meloidogyne spp. per soil sample Number of all roots Number The total number of all roots (both dead and functional) in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. mm plantannyield The bunch weight produced by the plant over the course of the crop cycle, averaged across one year. Plant annual yield Annual yield Time from start of crop cycle to first external disease symptoms caused by Fusarium wilt - computation Calculate as: the time elapsed between the Date of planting or the Date of harvest of the bunch from the plant of the previous crop cycle and the Date of first external disease symptoms caused by Fusarium wilt. finger Number of nematodes (Hoplolaimus spp.) per unit fresh root weight - method Count from 100 ml of soil sample Date of damage - estimation Record the date of event. Record the apparent cause of the damage to the plant, e.g. strong winds, weevil damage, etc. Cause of damage - estimation Number used to obtain a measurement Number of fingers measured The number of fingers used to obtain a measurement. numfingersmeas describedamage Damage description Description of damage A description of the damage to the plant. Presence/absence of collapse PC The presence/absence of petiole collapse, caused by Fusarium wilt. Presence of petiole collapse caused by Fusarium wilt No. per 10g Banana weevil adult body weight Body weight The weight of a banana weevil adult weevil body. The average actual annual yield, taking into account the proportion of plants harvested, from the number of plants planted actualannyield Average actual annual yield Average actual annual yield State description A description of the state of the plant. statedescription Plant state description Stickiness scale 0-10 Genotype selection traits Description of traits considered important in selection of best genotype by respondents Selection criteria for genotype ranked highest by respondents Alignment of bracts at the apex of the male bud Imbrication Bract imbrication at apex of male bud Finger circumference - measurement Measure the circumference of a finger at its widest point, using a tape measure. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. mechanical textural attribute relating to the force required to achieve a given deformation, penetration, or breakage of a product. Matooke Hardness in hand Matooke hardness MatHardT Overall plant appearance Overall plant appearance PA Assessment by respondents of physical features of a plant by giving it a score on the preference scale Cut multiple hands from the rachis and weigh them together, using scales. Associate the data with the Hank rank and the Number of hands measured. Multiple hand weight - measurement Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 and the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1, e.g. 24/06/2014 - 24/04/2014 = 61 days. Black leaf streak disease evolution time - computation The weight of a banana weevil larvae body. Body weight Banana weevil larvae body weight Youngest leaf spotted The rank (order) of the first fully unfurled leaf with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 leaf spot light-coloued dry centres, caused by black leaf streak disease. YLS Rank of youngest leaf spotted Boiled plantain Boiled plantain Mealiness Mealiness Mechanical property linked to cohesion and the presence of fine particles in the product during chewing BoiPlantnMeal datedatacoll The date of data collection. Date of data collection Date of data collection numsuckers Number of suckers The number of all types of suckers in the mat. Number of all types of suckers mm Head capsule width Banana weevil adult head capsule width The width of a banana weevil adult weevil head. Petiole margin clasping - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Observation should be made at shooting on the neck, where the petiole and pseudostem meet. Margin is the part of the petiole that can be bent outwards/inwards. [x Observe at flowering time.] End date and time of data collection - estimation Record the date and time of event. Visual assessment. Rating of external symptoms of Fusarium wilt in the field - estimation Fruit smashed pulp texture The texture of the smashed pulp of a mature fruit Texture Percentage of plants with petiole collapse caused by Fusarium wilt Combined percentage with petiole collapse Percentage of plants with petiole collapse, caused by Fusarium wilt. The number of functional roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Number Number of functional roots Date of first external disease symptoms caused by Fusarium wilt - estimation Record the date of event. plantcircumf100cm The circumference of the pseudostem of the plant at 100 cm from the collar. Plant circumference 100 cm from the collar Circumference 1 m above the ground Annual crop cycle proportion - computation Calculate as: the length of a year (e.g. 365 days) divided by the Plant crop cycle or the Ratoon crop cycle, e.g. 365 / 334 = 1.09. Sweetness Boiled plantain Sweetness Elemental flavor caused by dilute aqueous solutions of various substances such as sucrose or aspartame BoiPlantnSwt Boiled plantain Growth - computation Pseudostem height difference between timepoints (refer to experimental metadata) mL Matooke stickiness Stickiness in hand mechanical textural attribute relating to the force required to remove material that sticks to the mouth Matooke MatStickT Root relative water content rwcRoot The relative water content in a root Content The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the colour of the backside middle of tepal. Use colour chart B and observe out of direct sunlight. Compound tepal main colour - estimation Growth - computation Leaf area increase between timepoints (refer to experimental metadata) per number of new leaves formed between timepoints (refer to experimental metadata) Visually observe the fruit pulp colour and select the option in the colour chart that is most representative. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Fruit pulp colour - estimation Calculated number of fingers in bunch The estimated number of fingers in the bunch based on a calculation using the number of fingers in the third and second-most distal hands and the number of hands in the bunch. estnumfingers Number of fingers Focus group discussion for top traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked highest in preference ranking exercise Visual assessment. Presence of changes in new leaves caused by Fusarium wilt - estimation Moistness scale 0-10 hand Date and time of end of data collection The date and time of the end of data collection on a plant. dateendtimedatacoll End date and time of data collection The presence/absence of yellowing leaves, caused by Fusarium wilt. Presence of yellowing leaves caused by Fusarium wilt Presence/absence of yellowing YL peelthickness Finger peel thickness Thickness of the peel The thickness of the peel of a finger. Date of planting - estimation Record the date of event. Date of death - estimation Record the date of event. The extent to which vascular discolouration extends up the pseudostem of the tallest sucker should be determined by making cross-section cuts, from the base of the pseudostem upwards, and examining the internal tissues following each cut. The point at which discolouration is no longer visible should be noted and the distance from this point to the pseudostem base recorded. Extent of internal discolouration in corm of tallest sucker caused by Fusarium wilt - estimation Length The length of a leaf Single leaf length singleLeafLength Number of leaves with black leaf streak disease severity grade 3 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 3. Number of functional leaves - counting Count how many functional leaves (leaves that have 50% or more of their surface as green, healthy, photosynthetic tissue) are on the plant. Fusion of pedicels Fusion Degree of fusion of the pedicels before they join the rachis Bract imbrication at apex of male bud - estimation Visually observe the bract imbrication of the apex of the male bud and choose the option that is most similar, from the 3 photos of descriptor 6.5.3 in the reference material. Number of leaves with black leaf streak disease severity grade 4 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 4. Percentage of functional roots - computation Calculate as: the Number of functional roots divided by the Number of all roots, multiplied by 100, e.g. 12 / 20 * 100 = 60 % functional roots. astringency scale 0-10 Visual estimation of fruit pendulance on a bunch, for Early Evaluation Trial stage only. Fruit pendulance - estimation Time from planting to death Time from planting to death The time elapsed from planting to the death of the plant. plant2death Visually observe and record the amount of the surface area of a standing leaf that is affected by black leaf streak disease. Black leaf streak disease severity - estimation leavesFormedPerPseudostemGrowth Stunting expressed as number of leaves per height growth Leaves formed per pseudostem height increase Formation Dry weight - measurement 80?C, for 14 days, or longer untill dry Cut the hands off the rachis and weigh the rachis, using scales. Rachis weight - measurement Meredith and Lawrence (1969) - condensed Average finger internal length - computation Calculate as: the sum of the Finger internal length measurements, divided by the number of those measurements, e.g. (27 + 31 + 29 + 30 + 30 + 28) / 6 = 29.2. Finger weight - measurement Weigh a finger, using scales. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. bud shape scale (1 to 5) Calculate as: the sum of Damage to inner corm upper cross-section caused by weevils, Damage to outer corm upper cross-section caused by weevils, Damage to inner corm lower cross-section caused by weevils, and Damage to outer corm lower cross-section caused by weevils, divided by the number of traits included in the sum, e.g. (25 + 36 + 18 + 20) / 4 = 24.8. Average damage to the corm weevils - computation Number of Rotylenchulus spp.per soil sample Number of Rotylenchulus spp.in soil Population density The longest diagonal of a fitted ellips Leaf length - measurement numfingers3rdhand Number of fingers in 3rd hand Number of fingers The number of fingers in the third hand. Focus group discussion for third lowest ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked third lowest in preference ranking exercise Firmness Sensory Texture method Put in the mouth a piece of banana and Evaluate the force necessary to obtain the deformation of the product between the teeth during the first compression fruit relicts scale (1 to 3) Mealiness Sensory Texture method Put a piece of banana in your mouth and assess the presence of mealiness particles during chewing Record the date of event. Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 - estimation Dry weight Plant dry weight The dry weight of the whole plant. plantDryMass Pseudostem height Distance from the base of the pseudostem to the emerging point of the peduncle. Height Mealiness scale 0-10 Damage to upper outer corm weevils - measurement Cut a transverse cross section of the corm at the collar (upper cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Petiole margin colour - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Use colour chart A and observe out of direct sunlight. Record the colour of the margin (general colour is below the rim). [x Observe at flowering time.] Time from flowering to harvest Time from flowering to harvest flower2harvest The time elapsed from flowering (when the last bracts fall from the most distal hand to display the female flowers) to the harvest of the bunch. kg Average finger external length - computation Calculate as: the sum of the Finger external length measurements, divided by the number of those measurements, e.g. (30 + 34 + 32 + 33 + 33 + 31) / 6 = 32.1. Calculate as: 100 multiplied by (Youngest leaf spotted, minus 1), divided by Number of standing leaves, e.g. 100 * (6 - 1) / 12 = 42. Index of non-spotted leaves - computation Count how many fingers are used to obtain a measurement. Number of fingers measured - counting Measurement under microscope Weevil larvae head capsule - measurement The average width of a banana weevil larvae head capsule. Average head capsule width Banana weevil larvae average head capsule width Hardness in hand method Take a part of the sample between fingers and evaluate how hard the sample is deathprop Combined proportion of plants that are dead The proportion of plants that are dead, from the number of plants planted. Death proportion Volume waterLossPlant The amount of water loss through the plant over time Plant water loss Number of leaves with black leaf streak disease severity grade 0 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 0. peeper sucker Percentage of plants with yellowing leaves caused by Fusarium wilt - computation Calculate as: the sum of the number of values for Presence of yellowing leaves caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 78 / 300 * 100 = 26%. Leaf to whole plant mass ratio Measure for the biomass allocation: dry mass of leaves versus whole plant dry mass ratio leafToPlantMassRatio_dry Ratio The number of leaves on a plant with a black leaf streak disease severity grade of 4. Number of leaves with black leaf streak disease severity grade 4 Number of leaves with a black leaf streak disease severity grade of 4 Ratio rootToPlantMassRatio_dry Measure for the biomass allocation: dry mass of roots versus whole plant dry mass ratio Root to whole plant mass ratio Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards. [x Observe at flowering time.] Winged petiole margin - estimation Finger external length - measurement Measure the length of a finger that is at the ready to eat stage, along the external (dorsal) arc, excluding the pedicel and the fruit tip, using a tape measure. Associate the data with the Hand rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Number of nematodes (unclassified spp.) per unit fresh root weight - method Count from 100 ml of soil sample Average number of fingers in hand The average number of fingers in a hand. avenumfingshand Average number of fingers Leaf number The number of all leaves (including dried/dead/wilted/yellow) in a shoot system Number numLeaves Vitamin C content The Vitamin C content of freeze-dried smashed pulp of a mature fruit Vitamin C content kg Colour of bract internal face Main colour of the first external unlifted bract internal face Colour of the internal face Monthly leaf emission rate - computation Calculate as: the Rank of previously marked leaf at one point in time, minus 1 (equivalent to the Rank of marked youngest leaf), divided by the time elapsed between the two Date of data collection events when the marked leaf was recorded as 1) the Rank of marked youngest leaf and 2) the Rank of previously marked leaf, e.g. (4 - 1) / 1 month = LER of 3 leaves/month during a particular time period. plantheightpetioles Height Plant height The height of the pseudostem of the plant, from the collar to the intersection of the petioles of the two youngest leaves. BLS Black leaf streak disease severity Black leaf streak disease severity An indication of the extent of damage on an individual leaf caused by black leaf streak disease. bud shoulder scale (1 to 3) Fruit unsmashed pulp texture Texture The texture of the unsmashed pulp of a mature fruit Time from shooting to harvest - computation Calculate as: the time elapsed between the Date of shooting to the Date of harvest. E.g. 24/12/2014 - 24/09/2014 = 91 days. Calculate as: the sum of the Fruit pulp lateral diameter measurements, divided by the number of those measurements, e.g. (3.1 + 4.8 + 3.7 + 4.6 + 5.6 + 6.4) / 6 = 4.7. Average fruit pulp lateral diameter - computation Number used to obtain a measurement numhandsmeas Number of hands measured The number of hands used to obtain a measurement. A leaf weight trait which is the fresh weight of a leaf Leaf fresh weight leafFreshMass Fresh weight plant bunch maturity rwcPlant Plant relative water content Content The relative water content in the whole plant Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked second highest in preference ranking exercise Focus group discussion for second ranked traits with female respondents Nematode multiplication from single root inoculation - computation Calculation from count Astringency method Put a part of the sample in the mouth and evaluate the intensity of astringency impression due to the sample Content rwcPseudostem Pseudostem relative water content The relative water content in a pseudostem Fruit unsmashed pulp smoothness-mouth feel Smoothness-mouth feel The smoothness-mouth feel of the unsmashed pulp of a mature fruit color homogeneity scale 0-10 Hand weight - measurement Cut a hand from the rachis and weigh the hand, using scales. Associate the data with the Hand rank. Average internal length avefingerintlength Average finger internal length The average length of the fingers measured along the internal (ventral) arc, excluding the pedicel and the fruit tip. maiden sucker Record the date and time of event. Start date and time of data collection - estimation Number of hands on whole bunch - counting On a bunch with mostly hands of > 10 fingers, a possible ultimate hand with 1-5 (rather smaller) fingers should not be counted. Male bud shoulder - computation Calculate the ratio w/y, i.e. the broadest width of the male bud to the total length of the male bud. Do not measure the dimensions along the bud but rather on a projection/outline of the bud (e.g. trace the outline of the bud on paper) and use ruler or measuring tape Gram accumulated biomass per volume water transpired Transpiration efficiency - computation The amount of damage to the outer corm (cortex) upper cross-section, assessed by percentage of tunnels caused by weevils. Damage percent X-upper-outer Percentage damage to upper cross-section outer corm caused by weevils Fresh weight difference between timepoints (refer to experimental metadata) Growth - computation MatSurfHomColV Matooke homogeneity of surface colour Uniformity of color of the surface of the sample Matooke Surface color homogeneity Combined weight Total finger weight The weight of all the hands of the bunch, excluding the rachis. totalfingerweight Count from 100 ml of soil sample Number of nematodes (Pratylenchus spp.) per unit fresh root weight - method The average lateral (side to side) diameter of a finger. Average lateral diameter Average finger lateral diameter avefingerlatdiam Number of dead roots Number The number of dead roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Banana pest disease Focus group discussion for lowest ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked lowest in preference ranking exercise The time elapsed between the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1, to 6. DET Time elapsed from Foures black leaf streak disease severity scale 1 to 6 Black leaf streak disease evolution time leafAreaIncrease Increase of leaf area Growth Leaf area increase over time plants not harvested/plants planted The growth (weight increase) per volume transpired water Transpiration efficiency transpirationEfficiency Efficiency Identify the nematode species present in the banana plant root sample (Radopholus similis, Pratylenchus coffeae, Pratylenchus goodeyi, Helicotylenchus multicinctus, Meloidogyne incognita, ?) Nematode species - estimation Dry weight difference between timepoints (refer to experimental metadata) Growth - computation leaves/week Number of hands in bunch - counting Count how many hands are in the bunch. Number of nematodes (Pratylenchus goodeyi) per unit fresh root weight - method Count from 100 ml of soil sample Nematode root knot galling presence - estimation Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. From the functional roots, randomly select five primary roots that are at least 10 cm, and reduce their length to 10 cm. Determine the presence/absence of root knot nematodes, observed externally as galls on the roots. method_of yyyy/mm/dd Matooke aroma pumpkin Matooke Aroma of pumpkin. Possibly, precise if the taste is like boiled pumpkin or fried... Pumpkin-like aroma MatAroPumpA Mark the youngest completely unrolled leaf and record its rank, which is always 1. Associate the data with the Date of data collection. Rank of marked youngest leaf - counting leaf Date of data collection - estimation Record the date of event. The number of peeper suckers (suckers less than 15 cm tall) in the mat. numpeepersuckers Number of peeper suckers Number of peeper suckers Combined number of plants not harvested numnotharv Number of plants not harvested The number of plants that are alive but not harvested (e.g. due to slow or poor development). Bract behaviour before falling - estimation Visually observe the last bract to lift from the hands, ideally when the bract has lifted to a horizontal position, and choose the option that is most similar, from the 2 photos of descriptpr 6.5.12 in the reference material. totLeafLength Length Total length of all leaves The length of a leaf Calculate as: the time elapsed between the Date of harvest of the bunch of the plant from the preceeding crop cycle and the Date of death of the current plant, e.g. 24/03/2015 - 24/12/2014 = 90 days. Time from harvest to death - computation rootDryMass Root dry weight The dry weight of the root system Dry weight The date the bunch is harvested from the plant. Date of harvest Date of harvest dateharvest Genotype selection traits Description of traits considered important in selection of second best genotype by respondents Selection criteria for genotype ranked second highest by respondents Visually observe the male rachis position (the part between the last hand and the male bud) and choose the option that is most similar, from the 5 schematic drawings of descriptor 6.4.12 in the reference material. Male rachis position - estimation The taste of the smashed pulp of a mature fruit Taste Fruit smashed pulp taste wing presence/absence How many banana weevil adults caught per trap. Number of banana weevil adults Number of banana weevil adults per trap The extent of discolouration in the corm of the plant, caused by Fusarium wilt. Extent of internal discolouration in corm of plant caused by Fusarium wilt Extent of discolouration Count how many water suckers are in the mat. Number of water suckers - counting Visual counting method Number of adult larvae - counting Date of first youngest leaf spotted The date a youngest leaf spotted is first observed on the plant, caused by black leaf streak disease. Date of first youngest leaf spotted bud size scale (1 to 3) The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the colour of the lobe at the tip of the tepal. Use colour chart B and observe out of direct sunlight. Lobe colour of tip of compound tepal - estimation Number of leaves with black leaf streak disease severity grade 1 The number of leaves on a plant with a black leaf streak disease severity grade of 1. Number of leaves with a black leaf streak disease severity grade of 1 Record the rank (order) of the second-most distal hand in the bunch, starting with the hand at the proximal end (closest to the pseudostem) of the bunch as 1 and continuing to the hand at the most distal end (closest to the male bud). Hand rank - counting Finger circumference fingercircumf The circumference of a finger. Circumference Put a part of the product and by retro-olfaction evaluate the presence and the intensity of pumpkin-like aroma Pumpkin-like Aroma method Weight multhandweight Multiple hand weight The weight of multiple hands. Combined number of plants harvested Number of plants harvested numharv The number of plants that are harvested. Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked second lowest in preference ranking exercise Focus group discussion for second lowest ranked traits with male respondents Number of Pratylenchus spp. per fresh root weight Population density Number of Pratylenchus spp. in roots Preference scale Percentage of dead roots Percentage of dead roots The percentage of all roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant that are dead. Parthenocarpy The overall estimation of fruit parthenocarpy level on a bunch Parthenocarpy goodfruitfill The colour of the fruit pulp. fruitpulpcolour Fruit pulp colour Pulp colour Length [x at maturity] Fruit length Length of the internal arc of a finger ** subsume Chewiness BoiPlantnChew Energy or number of chews necessary to chew the banana to make it ready to be swallowed Boiled plantain Boiled plantain Chewiness bunchmaturity Stage of maturity at harvest The stage of maturity of the bunch at harvest. Bunch maturity stage at harvest The date a tagged cigar leaf is at Bruns stage B of unrolling. Date of tagged cigar leaf unrolling at Bruns stage B Date of tagging at Bruns stage B of unrolling dwarfism finger The time elapsed between a tagged cigar leaf at Bruns stage B of unrolling and the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1. Time elapsed from Bruns stage B of unrolling to Foures black leaf streak disease severity scale 1 Black leaf streak disease incubation time DIT Calculate as: the time elapsed between the Date of planting and the Date of shooting. E.g. 24/09/2014 - 24/01/2014 = 243 days. Time from planting to shooting - computation Average number of banana weevil adults Average number of banana weevil adults per trap The average of how many adult banana weevils caught per trap. Focus group discussion for third lowest ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked third lowest in preference ranking exercise fruit relicts scale (1 to 4) Average finger external length Average external length The average length of the fingers measured along the external (dorsal) arc, excluding the pedicel and the fruit tip. avefingerextlength Number of Helicotylenchus multicinctus per soil sample Population density Number of Helicotylenchus multicinctus in soil The presence of root knot nematodes, observed externally as galls on the roots. Nematode root knot galling presence Presence of root knot nematodes Population density Number of Meloidogyne spp. per fresh root weight Number of Meloidogyne spp. in roots Main colour Compound tepal main colour Main colour of the backside of the compound tepal Black leaf streak disease development time Time elapsed from Bruns stage B of unrolling to Foures black leaf streak disease severity scale 6 The time elapsed between a tagged cigar leaf at Bruns stage B of unrolling and the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6. DDT Percentage of plants with petiole collapse caused by Fusarium wilt - computation Calculate as: the sum of the number of values for Presence of petiole collapse caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 84 / 300 * 100 = 28%. Male rachis appearance - estimation Visually observe the male rachis appearance and choose the option that is most similar. Fruit shape of curve Shape of the longitudinal curvature Shape of a fruit (finger) in the middle of the bunch **subsume The time elapsed from shooting (when the inflorescence emerges from the pseudostem and is still in an erect position) to the harvest of the bunch. shoot2harvest Time from shooting to harvest Time from shooting to harvest Sucker quality - estimation Visual estimation of suckering, for Early Evaluation Trial stage only. Leaf number - counting Count the number of leaves (functional only > 50% estimated green area) The percentage of the root cortex of five functional roots approximately 10 cm long that is necrotic due to nematode damage. Percentage necrotic Root necrosis caused by nematodes Sweetness scale 0-10 nonharvprop The proportion of plants that are alive but not harvested (e.g. due to slow or poor development), from the number of plants planted. Combined proportion of plants not harvested Non-harvest proportion Open question 1 to 5 acceptability score Number of standing leaves The number of leaves attached to the plant that have an erect petiole (not bent or broken). Number of standing leaves Colour line along edge of petiole margin Presence or absence of colour line along edge of petiole margin Presence t/unit of area/y Vitamin C - measurement Calculate as: the sum of the number of values for Presence of petiole collapse caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 84 / 300 * 100 = 28%. The overall estimation of sucker quality on a mat suckering Overall suckering quality Sucker quality Count how many sword suckers are in the mat. Number of sword suckers - counting Number of banana weevil traps used How many How many banana weevil traps used. crop cycle duration/year Average number of fingers in hand - hands - computation Calculate as: the sum of the Number of fingers in hand, divided by the Number of hands in bunch, e.g. (10 + 10 + 11 + 10 + 10 + 10 + 12 + 12 + 12 + 11 +12 + 12 + 10 + 10 + 10 + 11) / 16 = 10.8. Single leaf width Width The width of a leaf singleLeafWidth Ratio - computation Ratio of belowground (root) to aboveground (shoot) dry mass Visually observe the fusion of pedicels and choose the option that is most similar, from the 3 photos of descriptor 6.7.11. in the reference materials. Observe before they join the rachis at the bract scar. Look upwards from the bottom of the bunch. Fusion of pedicels - estimation Record the date of event. Date of harvest - estimation BD colour chart A - 1996 Calculate as: the sum of values with a Black leaf streak disease severity grade of 5. Number of leaves with black leaf streak disease severity grade 5 - computation Population density Number of Hoplolaimus spp. in soil Number of Hoplolaimus spp. per soil sample The average circumference of the fingers. avefingercircumf Average finger circumference Average circumference Dry weight - measurement 80?C, for 14 days, or longer untill dry Stickiness Sensory Texture method Press a piece of banana between the molars and appreciate the adhesion of the product BD colour chart B - 2016 Calculate as: the sum of the number of values for Presence of splitting pseudostem base caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 24 / 300 * 100 = 8%. Percentage of plants with a splitting pseudostem base caused by Fusarium wilt - computation A rate that expresses the number of leaves that have emerged from the pseudostem in a month. Monthly leaf emission rate LER Monthly leaf emission rate Fresh weight - measurement Weighing Date of shooting - estimation Record the date of event. SP Presence/absence of splitting The presence/absence of a splitting pseudostem base, caused by Fusarium wilt. Presence of splitting pseudostem base caused by Fusarium wilt Dry weight Pseudostem dry weight The dry weight of the pseudostem pseudostemDryMass Water loss - measurement Weighing of water loss though system normalized by leaf area MatFirmM Mechanical textural attribute relating to the force required to achieve a given deformation, penetration, or breakage of a product. Matooke firmness Matooke Firmness in mouth Body length Banana weevil larvae body length The length of a weevil larvae body, from the weevil larvae head to the tip of the abdomen. Multiple finger weight - measurement Weigh multiple fingers together, using scales. Associate the data with the Hank rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. roots Water loss - measurement Weighing of water loss though plant between timepoints (refer to experimental metadata) Calculate as: the Plant density multiplied by the Non-harvest proportion, e.g. 1,667 * 0.08 = 133. Non-harvest density - computation Record the rank (order) of the oldest standing leaf (with an erect petiole), counting the rank by starting with the youngest completely unrolled leaf as 1 and moving downwards, associating the data with the Date of data collection. Rank of oldest standing leaf - counting Damage percent X-lower-outer Percentage damage to lower cross-section outer corm caused by weevils The amount of damage to the outer corm (cortex) lower cross-section, assessed by percentage of tunnels caused by weevils. Record a description of the state of the plant. Plant state description - estimation dd/mm/yyyy Finger internal length The length of a finger measured along the internal (ventral) arc, excluding the pedicel and the fruit tip. fingerintlength Internal length Area allocated to one plant plantspacing Plant spacing The area allocated to one plant. Subjective visual assessment of smashed and unsmashed fruit pulp Visual appearance - estimation Number of fingers in bunch - estimation Calculate as: the sum of the Number of fingers in 3rd hand and the Number of fingers in 2nd-most distal hand, divided by 2, and multiplied by the Number of hands in bunch, e.g. (16 + 12) / 2 * 8 = 112. Selection criteria for genotype ranked third lowest by respondents Description of traits considered important in selection of third least genotype by respondents Genotype selection traits Presence of petiole collapse caused by Fusarium wilt - estimation Visual assessment. Visually observe the part of the rachis between the last hand and the male bud. Truncated means there is no bract scar below the last hand. Present means there is a degenerated or persistent male bud Male rachis type - estimation Time from start of crop cycle to first external disease symptoms caused by Fusarium wilt The time elapsed from the start of the crop cycle to the appearance on the plant of any of the external disease symptoms - - yellowing leaves, splitting pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Time from start of crop cycle to appearance of external disease symptoms caused by Fusarium wilt bract behaviour scale (1 to 2) functional roots bud shape scale (1 to 3) Record the rank of the previously marked leaf (the leaf that was marked in the Rank of marked youngest leaf during the last data collection), counting the leaf rank by starting with the newest completely unrolled leaf as 1 and moving downwards. Associate the data with the Date of data collection. Rank of previously marked leaf - counting deathdens The number of plants that are dead, in a defined area. Death density Combined density of plants that are dead Preference score individual respondents Researchers calculate the preference score by respondents Measurement under microscope Adult weevil head capsule - measurement Calculate as: the sum of the number of plants with any or multiple of the external disease symptoms caused by Fusarium wilt - yellowing leaves, splitting of pseudostem base, changes in new leaves, petiole collapse - divided by the Number of plants planted * 100, e.g. 112 / 300 * 100 = 37%. Percentage of plants with external disease symptoms caused by Fusarium wilt - computation Bunch maturity stage - estimation Estimate maturity stage of the bunch. Percentage damage to lower cross-section inner corm caused by weevils Damage percent X-lower-inner The amount of damage to the inner corm (central cylinder) lower cross-section, assessed by percentage of tunnels caused by weevils. Measure the distance from the collar, or from the pseudostem base at the ground if the collar is not visible, to under the neck of the curved peduncle, using a measuring pole or sliding ruler. Plant height - neck of peduncle - measurement Put a part of the sample in mouth, chew it and after 5 chews, evaluate between tongue and palate the number and the size of the particles. Smoothness in mouth method Number of fingers in 3rd hand - counting Count how many fingers are in the third hand. Individual respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. Preference scoring scale individual respondents fruit curve shape (scale 1 to 6) avepulpdiam Average fruit pulp lateral diameter Average lateral diameter of the pulp The average lateral (side to side) diameter of the pulp of a fruit. Ratio root to shoot rootShootDry Ratio The ratio of dry mass of belowground (root) to abovegrund (shoot) biomass Description of traits considered important in selection of least genotype by respondents Genotype selection traits Selection criteria for genotype ranked lowest by respondents Number of nematodes (Meloidogyne spp.) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample plants/unit of area Measure the circumference of the pseudostem of the plant at 75 cm from the collar, or from the pseudostem base at the ground if the collar is not visible, using a tape measure. Plant circumference 75 cm - measurement nummaidensuckers Number of maiden suckers The number of maiden suckers (fully grown suckers with foliage leaves) in the mat. Number of maiden suckers Visual assessment. Presence of changes in new leaves caused by Fusarium wilt - estimation pseudostemHeightIncreasePerFormedLeaf Pseudostem height increase per leaf formed Stunting expressed as height growth per new formed leaf Growth Cut a finger in half, crosswise at its widest point, and on the cross-section measure the lateral diameter of the fruit pulp, from the left to the right side (not from the ventral to the dorsal side), using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Fruit pulp lateral diameter - measurement goodbunch The overall estimation of the bunch quality on a mat Overall bunch quality Quality Rating of internal symptoms of Fusarium wilt in the greenhouse - estimation Visual assessment. Calculate as: the sum of the the Finger weight measurements, divided by the number of those measurements, e.g. (150 + 146 + 147 + 152 + 140 + 146) / 6 = 147. Average finger weight - computation Presence absence 0 Number of fingers Number of fruits on the mid-hand of the bunch **subsume Number of fruits on mid-hand of the bunch The third leaf (Leaf III) is counted from the last leaf produced before [x bunch emergence] [shooting]. Cut the petiole halfway between the pseudostem and the leaf blade and examine the cross section. [x Observe at flowering time]. Margin behaviour on petiole canal of third leaf - estimation Visual assessment. Presence of yellowing leaves caused by Fusarium wilt - estimation The length of a pseudostem Length lengthPseudostem Pseudostem length Colour The color of the unsmashed pulp of a mature fruit Fruit unsmashed pulp colour Count from 100 ml of soil sample Number of nematodes (Radopholus similis ) per unit fresh root weight - method Meredith and Lawrence (1969) - complete scale_of Calculate as: the Bunch weight divided by 1,000, multiplied by the Annual crop cycle proportion, multiplied by the Harvest density, e.g. (50 / 1,000) * 1.09 * 1,250 = 68.13. Actual annual yield - computation Index of non-spotted leaves Ex: g/100 mL juice???? leaves/month The weight of a finger. Finger weight Weight fingerweight How many banana weevil larvae used to obtain a measurement. Number of banana weevil larvae measured Number of banana weevil larvae measured Damage to lower inner corm weevils - measurement Cut a transverse cross section of the corm at 10 cm below the collar (lower cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. plants harvested/plants planted Content The relative water content in a leaf rwcLeaf Leaf relative water content Colour Petiole margin colour Main colour of the petiole margin. Where the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards The cause of death of the plant. Cause of death causedeath Cause of death Finger lateral diameter Lateral diameter fingerlatdiam The lateral (side to side) diameter of a finger. dd/mm/yyyy hh:mm:ss Harvest proportion - computation Calculate as: the Number of plants harvested, divided by the Number of plants planted, e.g. 18 / 24 = 0.75. Count from 100 ml of soil sample Number of nematodes (Meloidogyne spp.) per unit fresh root weight - method Count how many of the standing leaves are spotted (with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres), considering all leaves in between and inclusive of, the youngest leaf and the oldest standing leaf. Number of spotted leaves - counting Fruit length - range - measurement Measure the length of the internal arc of a fruit, without pedicel. Record on the inner fruit in the middle of the mid-hand of the bunch. If there is an even number of hands, there will be two middle hands so use the upper hand that developed first. Record the range. Number of Pratylenchus spp. per soil sample Population density Number of Pratylenchus spp. in soil hands MatSurfYellV Surface yellowness intensity Matooke Color of the surface of the sample from light yellow to bright yellow Matooke surface yellowness intensity wing presence/absence and clasping presence/absence scale 1 to 5 The number of nematode individuals per unit of fresh root weight. Root nematode density Number of nematodes per unit of fresh weight Number of Hoplolaimus spp. per fresh root weight Population density Number of Hoplolaimus spp. in roots fruit pedicel scale (1 to 3) Finger feel assessment of smashed and unsmashed fruit pulp Finger feel - estimation Number of nematodes (Helicotylenchus multicinctus) per unit fresh root weight - method Count from 100 ml of soil sample Fruit shape of curve - estimation Visually observe the shape of the curve of the fruit and choose the option that is most similar, from the 5 photos of descriptor 6.7.4. in the reference material. Observe the inner fruit in the middle of the mid-hand of the bunch. In case of an asymmetric bunch that has straight and curved fruits, please indicate it in the note section and score only the fruit on the upper side of the bunch. Tallest sucker number of functional leaves The number of leaves on the tallest sucker that have 50 % or more of the leaf surface area as green, healthy, photosynthetic tissue. tallsuckererfunctleaves Number of functional leaves Chewiness Subjective taste assessment of smashed and unsmashed fruit pulp Taste - estimation Number of banana weevil larvae per trap Number of banana weevil larvae How many banana weevil larvae caught per trap. avehandweight Average hand weight The average weight of the hands. Average weight Water content - computation (Leaf Fresh Weight - Leaf Dry Weight) / Leaf Fresh Weight Bract apex shape - estimation Refers to the first external unlifted bract. Visually observe the apex shape of flattened bracts to determine shape and choose the option that is most similar, from the 4 photos of descriptor 6.5.2. in the reference material. Measure the lateral diameter of a finger from the left to the right side (not from the ventral to the dorsal side), at the widest point, using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger lateral diameter - measurement functleaves The number of leaves on the plant that have 50 % or more of the leaf surface area as green, healthy, photosynthetic tissue. Number of functional leaves Number of functional leaves Moisture in mouth method Put a part of the sample in the mouth, chew and evaluate the quantity of water within the sample. (Pseudostem Fresh Weight - Pseudostem Dry Weight) / Pseudostem Fresh Weight Water content - computation Average body weight Banana weevil larvae average body weight The average weight of a banana weevil larvae body. Pest and/or disease assessment and/or sample collection description - estimation Describe the pest and/or disease assessment and/or sample collection taking place at the time of data collection. kg Number of peeper suckers - counting Count how many peeper suckers are in the mat. Top view image of loose leaves Leaf area - measurement Fusarium wilt internal symptoms scale Collect all roots from a standard size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Carefully wash the soil from the roots with tap water. Cut the roots into 1 cm pieces. Take a subsample of 5 g, add 100 ml distilled water and store the roots at 4 degrees Celsius. Stain the egg masses by immersing the roots in 0.15 g/L phloxine B for 15 mins. Count the number of egg-laying females under a stereo microscope. Nematode egg-laying females extent - estimation 5 pt corm discolouration scale Cut a transverse cross section of the corm at the collar (upper cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Damage to upper inner corm weevils - measurement Growth - computation Leaf area difference between timepoints (refer to experimental metadata) Average finger peel thickness - computation Calculate as: the sum of the Finger peel thickness measurements, divided by the number of those measurements, e.g. (2 + 2 + 2 + 3 + 2 + 3 + 3) / 6 = 2. Put a piece of banana in your mouth, chew it and swirl it around your tongue to detect the sweet flavor Taste method Sweetness pseudostemToPlantMassRatio_dry Ratio Measure for the biomass allocation: dry mass of pseudostem versus whole plant dry mass ratio Pseudostem to whole plant mass ratio Date of first youngest leaf spotted - estimation Record the date of event. Tallest sucker height - measurement On the tallest sucker, measure the distance from the pseudostem base at the ground to the intersection of the petioles of the two youngest leaves (leaf ranks 1 and 2), using a measuring pole or sliding ruler. petiole-margin contrast Number of weevil larvae - counting Visual counting method Number of fingers in 2nd-most distal hand Number of fingers numfingers2nddistalhand The number of fingers in the second-most distal hand numplanted Combined number of plants planted The number of plants that are planted. Number of plants planted Annual plant productivity - computation Calculate as: the Bunch weight multiplied by the Annual crop cycle proportion, e.g. 48 * 1.09 = 52.32. Number of spotted leaves Number of spotted leaves The number of leaves with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres, caused by black leaf streak disease. Fresh weight - measurement Weighing mg Number of nematodes (Rotylenchulus spp.) per unit fresh root weight - method Count from 100 ml of soil sample pulp colour Individual male respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. Preference scoring scale male respondents Number of hands measured - counting Count how many hands are used to obtain a measurement. Harvest density - computation Calculate as: the Plant density multiplied by the Harvest proportion, e.g. 1,667 * 0.75 = 1,250. kg/plant/y Measure from the scar on the rachis until the beginning of the fruit. Record on the inner fruit in the middle of the mid-hand of the bunch. Tip: use string to measure or trace outline of fruit on paper. Record the range. Fruit pedical length - range - measurement The apparent cause of damage to the plant. Cause of damage Cause of damage causedamage Presence of changes in new leaves caused by Fusarium wilt The presence/absence of changes in new leaves - irregular pale margins, narrowing of lamina, burning plus ripping of lamina, lamina becoming more erect, caused by Fusarium wilt. CNL Presence/absence of changes Extent of root knot nematodes The extent of root knot nematodes, observed externally as galls on the roots. Nematode root knot galling extent Growth pseudostemHeightIncrease Pseudostem height increase over time Height increase of pseudostem Calculate as: the time elapsed between the Date of planting to the Date of harvest. E.g. 24/12/2014 - 24/01/2014 = 334 days. Plant crop cycle - computation Calculate as: the time elapsed between the Date of flowering to the Date of harvest, e.g. 24/12/2014 - 24/10/2014 = 61 days. Time from flowering to harvest - computation Behaviour of the petiole margins - winged or not winged. the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Petiole margins clasping Margins clasping or not clasping The area of a leaf singleLeafArea Leaf area Area Male bud shape - computation Calculate the ratio w/y, i.e. the broadest width of the male bud to the total length of the male bud. Do not measure the dimensions along the bud but rather on a projection/outline of the bud (e.g. trace the outline of the bud on paper) and use ruler or measuring tape. Rank of previously marked leaf rankprevleaf The current rank (position) of the leaf that was previously marked as the youngest leaf at the time of the last data collection event. Rank Bunch position - estimation Visually observe the angle of the bunch and choose the option that is most similar, from the 5 schematic drawings of descriptor 6.4.6 in the reference material. avefingerweight Average weight The average weight of the fingers. Average finger weight Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Pratylenchus coffeae) per unit fresh root weight -method BD colour chart A - 2016 Population density Number of Pratylenchus coffeae per fresh root weight Number of Pratylenchus coffeae in roots bud length scale (1 to 3) The number of leaves on a plant with a black leaf streak disease severity grade of 0. Number of leaves with black leaf streak disease severity grade 0 Number of leaves with a black leaf streak disease severity grade of 0 Behaviour Margin behaviour on petiole canal of third leaf Margin behaviour of the petiole canal of the third leaf before bunch. Number of Pratylenchus coffeae in soil Number of Pratylenchus coffeae per soil sample Population density hand Count how many of the leaves are standing leaves (without a bent or broken petiole) Number of standing leaves - counting Length Male bud length Length of male bud from end of rachis to apex of the bud The rank (order) of a the second-most distal hand in the bunch. handid2ndmostdistal Hand rank of 2nd-most distal hand Rank Calculate as: the Number of plants planted, minus the Number of plants dead, minus the Number of plants harvested, e.g. 24 - 4 - 18 = 2. Number of plants not harvested - computation Pest and/or disease assessment and/or sample collection options Pest/disease assessment/sample collection options pestdiseasesampleoptions The options to record the pest and/or disease assessment and/or sample collection done at the time of data collection. bad good Researchers calculate the preference score by female respondents Preference score female respondents Pest and/or disease assessment and/or sample collection description Pest/disease assessment/sample collection description pestdiseasesampledescription A description of the pest and/or disease assessment and/or sample collection being done at the time of data collection. clasping or not clasping Visual observation Peripheral damage - measurement Researchers calculate the preference score by male respondents Preference score male respondents Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 - estimation Record the date of event. smoothness scale 0-10 waterLossSystPerLeafArea The amount of water loss in the system normalized by the leaf area Volume System water loss per leaf area Root nematode density - computation Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant, do not include roots from adjacent plants. Carefully wash the soil from the roots with tap water. Cut the roots into 10 cm long pieces and dry with paper tissue. Take a subsample of 15 g, add 100 ml distilled water and store the roots at 4 degrees Celsius. Put the roots in 100 ml of distilled water in a kitchen blender. Macerate the roots 3 times for 10 secs (separated by 5 sec intervals). Pour the macerated suspension through 250, 106 and 40 micro-metre sieves and rinse the sieves with tap water. Using distilled water, collect in a beaker the nematodes from the 40 micro-metre sieve. Using distilled water, dilute to 200 ml the nematode suspension in a graduated cylinder. Blow air through the nematode suspension with a pipette (to homogenise the suspension). Take a subsample of 6 ml (counting dish) or 2 ml (counting slide). Count the nematodes in the counting dish (stereo microscope) or in the counting slide (light microscope). Calculate the final nematode population per unit of fresh root weight. Aromas Fruit smashed pulp aroma/smell The pulp aroma of the smashed pulp of a mature fruit aroma scale 0-10 2 pt scale Finger internal length - measurement Measure the length of a finger, along the internal (ventral) arc, excluding the pedicel and the fruit tip, using a tape measure. Associate the data with the Hand rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Faures stages of development of BLS disease scale (1987) Presence of yellowing leaves caused by Fusarium wilt - estimation Visual assessment. Measure from the scar on the rachis until the beginning of the fruit. Record on the inner fruit in the middle of the mid-hand of the bunch. Tip: use string to measure or trace outline of fruit on paper. Record the exact value. Fruit pedicel length - exact value - measurement Calculate as: the sum of the Finger lateral diameter measurements, divided by the number of those measurements, e.g. (3.5 + 5.2 + 4.1 + 5 + 6 + 6.8) / 6 = 5.1. Average finger lateral diameter - computation fusion of pedicel scale (1 to 2) Visual assessment. Presence of petiole collapse caused by Fusarium wilt - estimation Leaf area - measurement Top view image of a single leave Plant density - computation Calculate as: number of square metres in the area unit of choice, divided by the Plant spacing, e.g. 10,000 square metres in 1 hectare / 6 = 1,667 plants/ha. The length of a finger measured along the external (dorsal) arc, excluding the pedicel and the fruit tip. fingerextlength External length Finger external length The projected leaf area of a plant (photosynthetic surface) Projected top area topLeafArea Area Number of Radopholus similis in soil Number of Radopholus similis per soil sample Population density Blotches colour - estimation Observe visually the colour of the blotches on the upper leaf sheath. Male bud shoulder description Ratio Male bud shoulder Record the date of event. Date of flowering - estimation MatMoistM Matooke perception of moisture content of a food by the tactile receptors in the mouth and also in relation to the lubricating properties of the product Matooke moisture Moisture in mouth Non-harvest proportion - computation Calculate as: the Number of plants not harvested, divided by the Number of plants planted, e.g. 2 / 24 = 0.08. bract imbrication scale (1 to 3) harvest2harvest Ratoon crop cycle Ratoon crop cycle The time elapsed from the harvest of the bunch of the plant from the preceding crop cycle to the harvest of the bunch of the plant from the current crop cycle. Functional leaf number The number of functional leaves (greenness>50%) in a shoot system Number numFuncLeaves Date of damage Date of damage datedamage The date damage to the plant is first observed. Count how many fingers are in the outer whorl of the second-most distal hand. Number of fingers in outer whorl of 2nd-most distal hand - counting Number of functional roots - counting Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. Count the number of functional roots. The number of water suckers (suckers with broad leaves, a small rhizome, and a weak connection to the plant) in the mat. numwatersuckers Number of water suckers Number of water suckers Fruit apex point Description of fruit apex Apex point leaf leaf rank Cut the hands off from the rachis and weigh all the hands together, using scales. Total finger weight - measurement The number of leaves on a plant with a black leaf streak disease severity grade of 3. Number of leaves with a black leaf streak disease severity grade of 3 Number of leaves with black leaf streak disease severity grade 3 pulpdiam Lateral diameter of the pulp The lateral (side to side) diameter of the pulp of a fruit. Fruit pulp lateral diameter No. per 100ml Pseudostem height difference between timepoints (refer to experimental metadata) per number of new leaves formed between timepoints (refer to experimental metadata) Growth - computation Number of dead roots - counting Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. Count the number of dead roots. Peripheral damage caused by weevils Damage to periphery of peeled corm caused by weevils The percentage of damage to the periphery of the peeled corm, assessed by tunnels caused by weevils Number of fingers The number of fingers in multiple hands. multnumfingshand Multiple number of fingers in hand bract apex scale (1 to 5) rachisweight Weight The weight of the rachis. Rachis weight Off-type description - estimation Record a description of what made the plant recogniseable as an off-type. % Number of nematodes (Hoplolaimus spp.) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Measure the length (y) of a projection/outline of the male bud on paper, with a measuring tape or ruler. Male bud length - measurement dateplant The date of planting the plant. Date of planting Date of planting Put a part of the sample in your mouth, evaluate during the first bite (between molars) how hard is the sample. Firmness in mouth method Chlorosis Leaf trait correlated to the health stage of the leaf Leaf chlorosis leafChlorosis Combined percentage with internal symptoms of Fusarium wilt Percentage of plants with internal disease symptoms caused by Fusarium wilt The percentage of plants with any or multiple of the internal disease symptoms - discolouration in the corm, and/or discolouration in the pseudostem, caused by Fusarium wilt. margin behaviour scale (1 to 5) height score (1 to 3) Matooke sweetness basic taste produced by dilute aqueous solutions of natural or artificial substances such as sucrose Sweetness Matooke MatSweet firmness scale 0-10 Bunch quality - estimation Visual estimation of bunch quality, for Early Evaluation Trial stage only. stickiness scale 0-10 Electronic weighing method Adult weevil body - measurement When you receive the sample, observe the surface and evaluate the intensity of the color Surface yellowness intensity Appearance method The weight of a hand. Hand weight Weight handweight Number of Pratylenchus goodeyi in soil Number of Pratylenchus goodeyi per soil sample Population density Distance of internal discolouration in pseudostem of tallest sucker, caused by Fusarium wilt Distance of internal discolouration in pseudostem of tallest sucker caused by Fusarium wilt For the tallest sucker, the complete corm should be removed from the soil, the roots cut off and excess soil removed. Cross-sections of the corm should be cut (using a guillotine or other suitable device) to obtain five slices of equal thickness. The upper surface of each cut section should be examined and visually evaluate the extent of the vascular discolouration. Chewiness Sensory Texture method Place the sample in the mouth, chew it at the rate of one chewing per second and assess the number of chews before swallowing. Index of non-spotted leaves INSL Index of non-spotted leaves An index to express the proportion of standing leaves without the typical late-stage symptoms of black leaf streak disease, i.e. a black spot with a necrotic centre. This index provides an estimation of available photosynthetic leaf area prior to fruit filling and is a measure of resistance. It also corrects for the difference in the number of leaves produced by different types of bananas. Number of fingers in hand numfingershand Number of fingers The number of fingers in a hand. Measure the length of the internal arc of a fruit, without pedicel. Record on the inner fruit in the middle of the mid-hand of the bunch. If there is an even number of hands, there will be two middle hands so use the upper hand that developed first. Record the exact value. Fruit length - exact value - measurement Individual female respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. Preference scoring scale female respondents Pest and/or disease assessment and/or sample collection options - estimation Record the pest and/or disease assessment and/or sample collection taking place at the time of data collection. Average body length The average length of a banana weevil adult body, from the head to the tip of the abdomen. Banana weevil adult average body length finger Smoothness-mouth feel Fruit smashed pulp smoothness-mouth feel The smoothness-mouth feel of the smashed pulp of a mature fruit margin behaviour scale (1 to 4) BoiPlantnStic Force required to peel off the fraction of product adhering to the interior of the oral cavity Boiled plantain Boiled plantain Stickiness Stickiness Focus group discussion for third ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked third highest in preference ranking exercise plantheightneck The height of the pseudostem of the plant from the collar to the curved neck of the peduncle. Plant height to neck of peduncle Height avepeelthickness Average thickness of the peel Average finger peel thickness The average thickness of the peel of a finger. Put a part of the product and by retro-olfaction evaluate the presence and the intensity of matoke-like aroma Matooke-like Aroma method Moistness Mouth impression method Once the flavors are detected, moisture is felt by turning the product in the mouth fruit length scale (1 to 5) Record the apparent cause of death of the plant, e.g. strong winds, Fusarium wilt, etc. Cause of death - estimation The time elapsed from planting to the harvest of the bunch. plant2harvest Plant crop cycle Time from planting to harvest blotch pigmentation scale (1 to 4) rachis angle scale (1 to 2) numfingersouterwhorl2nddistalhand Number of fingers in outer whorl The number of fingers in the outer whorl of the second-most distal hand Number of fingers in outer whorl of 2nd-most distal hand Population density Number of Radopholus similis per fresh root weight Number of Radopholus similis in roots plants dead/plants planted Banana weevil adult body length Body length The length of a banana weevil adult body, from the head to the tip of the abdomen. Shape of the first external unlifted bract of the male bud Apex shape Bract apex shape Bunch weight - measurement Harvest the bunch by cutting the peduncle 10 cm above the first ridge above the most proximal, hand and immediately below the most distal hand and weigh the bunch (including the rachis), using scales. Count how many fingers are in the third hand. Number of fingers in 2nd hand - counting The average width of a banana weevil adult head. Average head capsule width Banana weevil adult average head capsule width The date of the appearance on the plant of any of the external disease symptoms - yellowing leaves, splitting pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Date of appearance of external symptoms caused by Fusairum wilt Date of first external disease symptoms caused by Fusarium wilt Visual counting method of Weevils per trap (the trap should be minimum 30 cm long) Number of adult weevils - counting Death proportion - computation Calculate as: the Number of plants dead, divided by the Number of plants planted, e.g. 4 / 24 = 0.17. Main colour of the pseudostem under the outermost sheath. Main underlying colour of pseudostem Main underlying colour m2 Calculate as: the time elapsed between the Date of planting and the Date of death, e.g. 24/05/2014 - 24/01/2014 = 120 days. Time from planting to death - computation Average body weight The average weight of a banana weevil adult body. Banana weevil adult average body weight moldability scale 0-10 Date of recognising off-type dateofftype The date the plant is first recognised as an off-type. Date of recognising off-type Calculate as: the sum of the Finger circumference measurements, divided by the number of those measurements, e.g. (7.0 + 8.0 + 9.0 + 10.0 + 7.0 + 9.5) / 6 = 8.4. Average finger circumference - computation Number of maiden suckers - counting Count how many maiden suckers are in the mat. Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. From the functional roots, randomly select five primary roots that are at least 10 cm, and reduce their length to 10 cm. Where root knot nematode galls are present, determine the extent of the galling using the scale. Nematode root knot galling extent - estimation Calculate as: multiply the distance of the spacing between the plants, both latitudinally and longitudinally, e.g. 2 m x 3 m = 6 m2. Plant spacing - computation Dwarfism - estimation Visual estimation of dwarfism, for Early Evaluation Trial stage only. Visually observe the fruit apex point and choose the option that is most similar, from the 5 photos of descriptor 6.7.6 in the reference material. Fruit apex point - estimation sword sucker The date the assessment of weevil damage to the corm of the plant is conducted. Date of weevil damage assessment Date of weevil damage to corm assessment Combined percentage with external symptoms of Fusarium wilt The percentage of plants with any or multiple of the external disease symptoms - yellowing leaves, splitting of pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Percentage of plants with external disease symptoms caused by Fusarium wilt Measurement under microscope Weevil larvae body weight - measurement Blotches colour at the petiole base Main colour Colour of the blotches on the upper leaf sheath of the petiole base. Pseudostem colour - estimation Detach the outermost sheath from the pseudostem (the sheath should not be too dry). Record the overall impression of colour of the exposed surface of the underlying pseudostem. Note that this main colour should cover more than 75% of the underlying pseudostem surface. Use colour chart A and observe out of direct sunlight. Number of all roots - computation Calculate as: the Number of dead roots plus the Number of functional roots, e.g. 8 + 12 = 20 roots. leaf rank Calculate as: the sum of values with a Black leaf streak disease severity grade of 2. Number of leaves with black leaf streak disease severity grade 2 - computation mL / cm? blotch color scale (1 to 5) Non-harvest density Combined density of plants not harvested The number of plants that are alive but not harvested (e.g. due to slow or poor development), in a defined area. nonharvdens Fresh mass accumulation over time growthFresh Growth Fresh plant growth Shape of male bud Shape Male bud shape Nosal smell - estimation Nosal smell assessment of smashed and unsmashed fruit pulp aromas Selection criteria for genotype ranked third highest by respondents Description of traits considered important in selection of third best genotype by respondents Genotype selection traits Number of plants planted - computation Calculate as: the sum of the number of values for Date of planting. Number of fingers in bunch - computation Calculate as: the sum of the Number of fingers in hand measurements from all the hands in the bunch (number equivalent to Number of hands in bunch), e.g. 16 + 17 + 19 + 18 + 19 + 18 + 17 + 17 + 16 + 14 + 14 + 13 = 198. Fusarium wilt external symptoms scale Boiled plantain Perception of the amount of water absorbed or released by the product Moistness BoiPlantnMois Boiled plantain Moistness Date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1 The date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1. Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 Put a part of the product and by retro-olfaction evaluate the presence and the intensity of grassy-like aroma Grassy-like Aroma method fruit curve shape (scale 1 to 5) sucker Leaf length - measurement The longest diagonal of a fitted ellips Put a part of the sample in the mouth and evaluate the intensity of taste of sweetness Sweetness tasting method Harvest proportion The proportion of plants that are harvested, from the number of plants planted. Combined proportion of plants harvested harvprop Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Pratylenchus spp.) per unit fresh root weight -method geometrical textural attribute relating to lack of presence of particles in a product Matooke Smoothness in mouth Matooke smoothness MatSmoothM Rating of external symptoms of Fusarium wilt in the field Leaf yellowing and wilting caused by Fusarium wilt rating A rating of the extent of leaf yellowing and wilting, caused by Fusarium wilt. Wing presence Petiole margins winged Behaviour of the petiole margins - winged or not winged. the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Fresh weight - measurement Weighing Moldability in hand method Try to make a ball (agglomerate) of the sample and evaluate how easy it is to deform or break the sample Damage percent X-average Average percentage damage to the corm caused by weevils The average percentage of damage in the whole corm (the inner corm and the outer corm) caused by weevils. Focus group discussion for third ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked third highest in preference ranking exercise Record the date of event. Date of recognising off-type - estimation Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 and the Date of tagged cigar leaf unrolling at stage B, e.g. 24/04/2014 - 24/03/2014 = 31 days. Black leaf streak disease incubation time - computation Leaf area - measurement Top view image Index of black leaf streak disease severity Remains of flower relicts at fruit apex - estimation Visually observe the remains, or not, of flower relicts at the finger apex, and choose the option that is most similar, from the 4 photos of descriptor 6.7.7 in the reference materials. Root knot nematode galling Leaf number - counting Count the number of leaves rachis appearance scale (1 to 5) Fresh weight - measurement Weighing numhands Number of hands Number of hands in bunch The number of hands in the bunch. Manual height measurement Length - measurement sweetness scale 0-10 Number of nematodes (Radopholus similis ) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample moisture scale 0-10 Recorded from the base of the pseudostem to the emerging point of the peduncle. Pseudostem height - estimation sourness scale 0-10 Number of fruits on mid-hand of the bunch - counting Count only fully developed fruit. If there is an even number of hands, there will be two middle hands. Count the middle hand that developed first. Calculate as: the sum of the Hand weight measurements from all the hands in the bunch (number equivalent to Number of hands in bunch), e.g. 2.4 + 3.6 + 3.2 + 4.1 + 4.0 + 3.9 + 3.6 + 3.3 + 3.3 + 2.9 + 2.9 + 2.8 = 40.0. Total finger weight - hands - computation Gauhls modification of Stovers severity scoring system (YYYY) Percentage of plants with a splitting pseudostem base caused by Fusarium wilt Percentage of plants with a splitting pseudostem base, caused by Fusarium wilt. Combined percentage with splitting pseudostem base Ratio of non green to green plant pixels Leaf chlorosis - computation Number of plants dead - computation Calculate as: the sum of the number of values for Date of death. The extent of egg-laying female root knot nematodes, observed internally as pit-like structures on the roots. Nematode egg-laying females extent Extent of root knot nematodes The number of leaves on a plant with a black leaf streak disease severity grade of 6. Number of leaves with black leaf streak disease severity grade 6 Number of leaves with a black leaf streak disease severity grade of 6 Length of the fruit pedicel from rachis to the beginning of the pulp Length Fruit pedicel length yellowness scale 0-10 bunch shape scale (1 to 5) Put a part of the sample in the mouth and evaluate the intensity of the sourness Sourness method Combined number of plants that are dead numdead Number of plants dead The number of plants that are dead. Colour of bract external face - estimation Visually observe the colour of the external face of the first unlifted bract. Use colour chart A and observe out of direct sunlight. Average hand weight - all - computation Calculate as: the Total finger weight, divided by the Number of hands in bunch, e.g. 46.8 / 16 = 2.9. Focus group discussion for top traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked highest in preference ranking exercise Death density - computation Calculate as: the Plant density multiplied by the Death proportion, e.g. 1,667 * 0.17 = 283. The amount of damage to the inner corm (central cylinder) upper cross-section, assessed by the percentage of tunnels caused by weevils. Damage percent X-upper-inner Percentage damage to upper cross-section inner corm caused by weevils hardness scale 0-10 []/cm blotch pigmentation scale (1 to 5) Percentage of plants with yellowing leaves, caused by Fusarium wilt. Percentage of plants with yellowing leaves caused by Fusarium wilt Combined percentage with yellowing leaves variable_of Number of sword suckers The number of sword suckers (suckers with narrow leaves and a large rhizome) in the mat. numswordsuckers Number of sword suckers