Rachel Chase MlBdShp_Comp_1to3 Bioversity International Bioversity International Rhiannon Crichton AnCrpCyclPrp_Comp_CrpCclY Bioversity International Rhiannon Crichton SptPsdStmPercFW_Comp_pct 1 straight (or slightly curved) Bioversity International DateColl_Date_dmy Rhiannon Crichton Inge Van den Bergh PetColpPres_Est_1or2 Bioversity International PltDnsty_Comp_PlntHa Bioversity International Rhiannon Crichton Moist 10 3 watery green Matooke sweetness measurement scale 0-10 Elizabeth Kakhasa MatSweet_Meas_0to10 NARO, Uganda 7 Other PulpDiam_M_mm Bioversity International Rhiannon Crichton Bioversity International BlkLfStrkSvrtLfNb_Ct_grd1 Rhiannon Crichton Inge Van den Bergh Bioversity International LfChgPres_Est_0or1 5 curved in slight S shape (double curvature) IITA RadoNumSlWt_Comp_Nema Danny Coyne Allan Brown IITA Sckr_Est_1to2 Selection criteria for genotype that was ranked third highest by male respondents during preference scoring exercise Noel Madalla Bioversity 2 straight in the distal part 3 black-purple FrtNb_Ct_gFruit Bioversity International Rhiannon Crichton Danny Coyne HoploNumSlWt_Comp_Nema IITA Bioversity International Rhiannon Crichton FrtExtLng_M_cm PltDead_Ct_Plnt Bioversity International Rhiannon Crichton PltSpcg_Comp_m2 Bioversity International Rhiannon Crichton IITA Allan Brown/Guillaume Bauchet (temp.) VitCCnt_M_ Allan Brown IITA Dwfsm_Est_1to2 temp8 Bioversity International Rhiannon Crichton Bioversity International Rhiannon Crichton CauDam_Est_txt Bioversity International DIT_Comp_d Rhiannon Crichton Rachel Chase FrtCvShp_Est_1to5 Bioversity International KU Leuven growthDry_Comp_g plantGrowth_dry Sebastien Carpentier growthDry Bioversity International Rhiannon Crichton temp21 Allan Brown/Guillaume Bauchet (temp.) FngSmshdPlpSmth_Est_1to5 IITA-BTI LfEmRate_Comp_LfWk Bioversity International Rhiannon Crichton 4 neutral/male flowers and presence of withered bracts on the entire stalk IITA-Bioversity International BlkLfStrkSvrt_Est_Gauhl FlwHvstTime_Comp_d Bioversity International Rhiannon Crichton Inge Van den Bergh/Danny Coyne Bioversity International/IITA FuncRtNum2_Ct_Rt IITA PratyGooNumRtWt2_Comp_Nema Danny Coyne Bioversity International Rhiannon Crichton AvgPulpDiam_M_mm Bioversity International Rhiannon Crichton MSuckN_Ct_MSuck temp16 Bioversity International Rhiannon Crichton areaPlant_per_areaTot KU Leuven leafAreaRatio_Comp_unitless areaRatio Sebastien Carpentier erect 5 purple to blue 3 IITA-BTI FngUnSmshdPlpTst_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) Bioversity International Rachel Chase CigLfPigm_Est_1to3 High shouldered (x/y less than or equal to 0.28) 1 PratyGooNumSlWt_Comp_Nema IITA Danny Coyne pseudostemHeightIncreasePerFormedLeaf_Comp_cm KU Leuven growthPS_per_leafFormed Sebastien Carpentier IITA-Bioversity International BlkLfStrkSvrt_Est_Faure 6 weevils fat (w/y equal to or more than 0.55) 3 temp31 Bioversity International Rhiannon Crichton DateColl_Date_ymd IITA-BTI Guillaume Bauchet 2 slightly curved Bioversity International BrctClExtFc_Est_1to10 Rachel Chase DeathCause_Est Bioversity International Rhiannon Crichton 10 hard Shoot_Date_ymd IITA-BTI Guillaume Bauchet moderately imbricate 2 Tendo Ssali IITA DmgPrphl_M_% Guillaume Bauchet OffTypEst_Date_ymd IITA-BTI Guillaume Bauchet PltEst_Date_ymd IITA-BTI 2 green Inge Van den Bergh/Danny Coyne Bioversity International/IITA temp45 Bioversity International Rachel Chase PMargWing_Est_1or5 soft 0 Selection criteria for genotype that was ranked third highest by female respondents during preference scoring exercise Noel Madalla Bioversity Bioversity International Rhiannon Crichton FstExtDiSmptFW_Date_ymd 3 margins curved inward Noel Madalla Selection criteria for genotype that was ranked highest by female respondents during preference scoring exercise Bioversity no visual leaf symptoms 1 rwcPseudostem KU Leuven rwcPseudostem_Comp_unitless ps_rwc Sebastien Carpentier slightly pointed 2 Bioversity International Rhiannon Crichton temp23 SuckHtShtStg_M_cm Bioversity International Rhiannon Crichton KU Leuven heightPS lengthPseudostem_Meas_cm Sebastien Carpentier heightPseudostem temp42 Tendo Ssali IITA PltDth_Comp_d Bioversity International Rhiannon Crichton ** 2 WvlLvMsrd_Ct_weevil Tendo Ssali IITA homogeneous 10 medium intensity 5 Bioversity International CmpdTplMnCl_Est_1to16 Rachel Chase Rhiannon Crichton temp40 Bioversity International 3 Second-streak stage: streaks change colour from reddish brown to dark brown or black, sometimes with a purplish tinge, clearly visible at the upper surface of the leaf. Allan Brown IITA GdBnch_Est_1to2 NARO, Uganda Matooke sourness measurement scale 0-10 Elizabeth Kakhasa MatSour_Meas_0to10 FrtDiam_M_mm Bioversity International Rhiannon Crichton Rhiannon Crichton Bioversity International BlkLfStrkSvrtLfNb_Ct_grd5 BoiPlantnFirm_Meas_0to10 CIRAD / UNA, Côte d’Ivoire Boiled plantain Firmness scale 0-10 Hermann Antonin KOUASSI 2 dislike areaPerLeaf Sebastien Carpentier KU Leuven singleLeafArea_Meas_cm2 areaSingleLeaf KU Leuven waterLossSystPerLeafArea_Meas_mlcm2 waterLossSystem_per_Area Sebastien Carpentier WvlAdltPrTrp_Ct_weevil Tendo Ssali IITA 1 open with margins spreading medium intensity 5 HndFrtNum_Ct_Frt Bioversity International Rhiannon Crichton Shoot_Date_dmy Bioversity International Rhiannon Crichton FngUnSmshdPlpCl_Est_1to5 IITA-BTI Allan Brown/Guillaume Bauchet (temp.) AvgWvlAdltBdLgt_M_µM Tendo Ssali IITA Matooke moisture in mouth measurement scale 0-10 MatMoistM_Meas_0to10 Elizabeth Kakhasa NARO, Uganda Bioversity International DthDnst_Comp_PlntHa Rhiannon Crichton Bioversity International Rhiannon Crichton HndRnk_Ct_HndRnk Bioversity International Rhiannon Crichton PlntShtTime_Comp_d Bioversity International Rhiannon Crichton temp12 Bioversity International BrctApxShp_Est_1to5 Rachel Chase Danny Coyne HelicoNumSlWt_Comp_Nema IITA Bioversity International Rachel Chase BrctClExtFc_Int_1to16 6 medium green Tendo Ssali IITA AvgWvlAdltBdWght_M_mg 2 medium shouldered (0.28 < x/y < 0.30) Allan Brown/Guillaume Bauchet (temp.) FngSmshdPlpCl_Est_1to5 IITA-BTI Bioversity International MlRchsPst_Estm_1to5 Rachel Chase FngSmshdPlpTst_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) IITA-BTI Amos Alakonya-Rhiannon Crichton IITA-Bioversity International SptLfNb_Ct_leaf Noel Madalla Bioversity Selection criteria for genotype that was ranked second highest by male respondents during preference scoring exercise PdcFsn_Est_1to2 Rachel Chase Bioversity International Guillaume Bauchet IITA-BTI FrtIntLng_M_cm Inge Van den Bergh Bioversity International PStmDiscol_Est_cm IITA-BTI FrtGrth_M_cm Guillaume Bauchet 5 brown/rusty brown Bioversity International Rhiannon Crichton CigLfUnrBrnStgB_Date_ymd KU Leuven rwcLeaf_Comp_unitless rwcLeaf leaf_rwc Sebastien Carpentier First streak stage: the initial speck elongates, becoming slightly wider, to form a characteristic narrow, reddish brown streak up to 20 mm long and 2.0 mm wide, with the long axis parallel to the leaf venation. At this stage, streaks are more clearly visible on the lower surface of the leaf than on the upper one. The distribution of streaks is variable. Sometimes they are most numerous near the edge of the left side of the leaf. At other times they are equally numerous on both sides of the leaf, and more or less evenly distributed. Frequently they are densely aggregated in a band several cm wide on one side or both sides of the mid-rib, becoming less numerous towards the edge of the leaf. Streaks may be so numerous that every cm2 of leaf surface bears one or more. There is considerable variation in the length of individual streaks on a given leaf, and they frequently overlap to form larger, compound streaks. 2 1 hanging vertically AvgPeelThck_M_mm Bioversity International Rhiannon Crichton Bioversity International Rhiannon Crichton PtYd_Comp_TnHaY relativeLeaf_dry KU Leuven massDryratioLeaf Sebastien Carpentier leafToPlantMassRatio_Comp_unitless pseudostemToPlantMassRatio_Comp_unitless KU Leuven massDryratioPseudostem Sebastien Carpentier relativePS_dry Bioversity International Rhiannon Crichton temp13 Selection criteria for genotype that was ranked third lowest by male respondents during preference scoring exercise Noel Madalla Bioversity AvgWvlLvBdLgt_M_µM Tendo Ssali IITA Noel Madalla Bioversity Selection criteria for genotype that was ranked second lowest by female respondents during preference scoring exercise Bioversity International Rhiannon Crichton AvgFrtGrth_M_mm Bioversity International MlRchsApp_Estm_1to7 Rachel Chase 4 ovoid 3 other (specify on answer sheet) 6 Small bunch from neutral/hermaphrodite flowers just above the male bud Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd2 Bioversity International IITA-BTI Guillaume Bauchet FrtDiam_M_cm 0 heterogeneous Tendo Ssali IITA DmgIdxOut_M_% extensive pigmentation (>50%) 4 HvstDth_Comp_d Bioversity International Rhiannon Crichton 1 brown low intensity 0 black (anthers aborted) 7 Sebastien Carpentier waterLossDaily waterLossDay_Comp_ml rateWaterLossPerDay KU Leuven Bioversity International Rhiannon Crichton FstYngLfsptd_Est_ymd 1 truncated RkPvsMktLf_Comp_leafrank Bioversity International Rhiannon Crichton cylindrical 1 WvlAdltMsrd_Ct_weevil Tendo Ssali IITA WvlLvBdWght_M_mg Tendo Ssali IITA good 2 BnchFrtNum_Comp_Frt Bioversity International Rhiannon Crichton BnchFrtNum_Ct_Frt Bioversity International Rhiannon Crichton pink-purple 8 NtHvstPrp_Comp_PlntNb Bioversity International Rhiannon Crichton IITA-BTI FngSmshdPlpTxt_Est_1to5 Allan Brown/Guillaume Bauchet (temp.) red-purple 9 21-30 cm 2 yellow 4 3 equal to or more than 3 m 5 neutral/male flowers on the whole stalk without persistent bracts (still firmly attached to the rachis) pseudostemDryMass_Meas_g KU Leuven ps_dry Sebastien Carpentier massDryPseudostem Bioversity International Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd0 Bioversity International Rhiannon Crichton BnchHndNum_Ct_Hnd massDryRootShoot KU Leuven rootShootDry_Comp_unitless Sebastien Carpentier rootShootRatioDry 2 First streak stage: specks elongate, becoming slightly wider to form narrow reddish-brown streaks. Noel Madalla Bioversity PA_EstFemale_Txt ExtDisProp_Comp_pct Inge Van den Bergh Bioversity International Rachel Chase BnchPst_Estm_1to5 Bioversity International 10 smooth Danny Coyne IITA NmRpFctr_Ct_Nema CigLfUnrBrnStgB_Date_dmy Rhiannon Crichton Bioversity International Bioversity International Rachel Chase PsdHght_E MeloiNumSlWt_Comp_Nema IITA Danny Coyne Bioversity International FrtWtAvg_M_g Rhiannon Crichton Matooke firmness in mouth measurement scale 0-10 Elizabeth Kakhasa NARO, Uganda MatFirmM_Meas_0to10 1-5% of lamina with symptoms 2 orange red 10 9 orange-red FngUnSmshdPlpSmth_Est_1to5 IITA-BTI Allan Brown/Guillaume Bauchet (temp.) Stage 4 appears on the underside as a brown spot and on the upper side as a black spot. 4 Rachel Chase Bioversity International AnthrCl_Est_1to8 Allan Brown IITA PdltFrt_Est_1to2 no contrast between margin and petiole (without a colour line along) 1 DateHarv_Date_dmy Bioversity International Rhiannon Crichton Inge Van den Bergh/Danny Coyne Bioversity International/IITA temp53 SSuckN_Ct_SSuck Bioversity International Rhiannon Crichton margins spreading 1 Rachel Chase MlBdShd_Comp_1to3 Bioversity International Inge Van den Bergh/Danny Coyne NmNumRtWt Bioversity International/IITA 11-30 egg masses 3 numLeaf totalNumLeaves Sebastien Carpentier numLeaves_Count_unitless KU Leuven MatAro_Meas_0to10 Elizabeth Kakhasa Matooke aroma measurement scale 0-10 NARO, Uganda temp11 Bioversity International Rhiannon Crichton Bioversity International Rhiannon Crichton PltHvstd_Ct_Plnt 4 31-100 egg masses Altus Viljoen Stellenbosch University temp37 Matooke grassy aroma measurement 2pt scale Elizabeth Kakhasa NARO, Uganda MatGrass_Meas_2pt 5 other BoiPlantnSwt_Meas_0to10 Hermann Antonin KOUASSI CIRAD / UNA, Côte d’Ivoire Boiled plantain Sweetness scale 0-10 lengthSingleLeaf lengthPerLeaf KU Leuven singleLeafLength_Meas_cm Sebastien Carpentier AnthrCl_Est_1to16 Rachel Chase Bioversity International Bioversity International Rachel Chase PMargCol_Esr_1to16 AnPltPrp_Comp_KgHaY Bioversity International Rhiannon Crichton margins overlapping 5 Rachel Chase Bioversity International BlotchCol_Est_1to5 Bioversity International Rhiannon Crichton AcAnYd_Comp_TnHaY Bioversity International PStmCol_Est_1to16 Rachel Chase BrctClExtFc_Int_1to8 Bioversity International Rachel Chase no symptoms 0 2 11-20 mm Bioversity International Rhiannon Crichton RkYgstLf_Comp_leafrank temp43 Tendo Ssali IITA SuckNum_Ct_Suck Bioversity International Rhiannon Crichton Bioversity International PtlClpFW_Comp_pct Rhiannon Crichton straight 1 IITA-BTI Allan Brown/Guillaume Bauchet (temp.) FngUnSmshdPlpTxt_Est_1to5 Inge Van den Bergh/Danny Coyne temp51 Bioversity International/IITA DateDeath_Est_ymd Guillaume Bauchet IITA-BTI 1 falling vertically Second-spot stage: the dark brown or black central area of the spot becomes slightly depressed and the water-soaked border becomes more pronounced due to darkening. At this stage, a slight yellowing of the leaf tissue immediately surrounding the water-soaked border may occur. 5 10 bright yellow Rachel Chase Bioversity International FrtLngth_M_cm 2 isolated points of discolouration in vascular tissue Inge Van den Bergh Bioversity International LfChgPres_Est_1or2 Bioversity International Rhiannon Crichton ChgNwLfPercFW_Comp_pct 1 add** Bioversity International Rhiannon Crichton BlkLfStrkInfIndex_Comp_Idx Bioversity International/IITA temp50 Inge Van den Bergh/Danny Coyne IITA-Bioversity International BlkLfStrkSvrt_Est_MedLawCdsd Dry 0 2 truncate ( DamEst_Date_dmy Bioversity International Rhiannon Crichton WvlLvHdCps_M_µM Tendo Ssali IITA Noel Madalla Bioversity Selection criteria for genotype that was ranked lowest by female respondents during preference scoring exercise temp15 Rhiannon Crichton Bioversity International Elizabeth Kakhasa MatPump_Meas_2pt NARO, Uganda Matooke pumpkin aroma measurement 2pt scale Bioversity International Rhiannon Crichton CropCyc_Comp_d Tendo Ssali IITA AvgWvlLvHdCps_M_µM BlkLfStrkLfFrStg1_Date_dmy Bioversity International Rhiannon Crichton Tendo Ssali IITA WvlLvPrTrp_Ct_weevil Bioversity International Rhiannon Crichton FlwT_Date_dmy Danny Coyne IITA NmNumSlWt Matooke surface yellowness measurement scale 0-10 MatSurfYellV_Meas_0to10 NARO, Uganda Elizabeth Kakhasa BnchShp_Estm_1to5 Bioversity International Rachel Chase 2 adult female Tendo Ssali IITA WvlAdlt_Ct_weevil 1 orange-brown (mahogany, like in Pisang Mas) temp38 Altus Viljoen Stellenbosch University 1/3-2/3 discoloured 4 dont know 2 Sebastien Carpentier leaves_per_heightPS leavesFormedPerPseudostemGrowth_Comp_percm KU Leuven BnchMatStg_Est_1to2 Guillaume Bauchet IITA-BTI Bioversity International Rhiannon Crichton temp41 MltFrtWt_M_gFruit Bioversity International Rhiannon Crichton 13-16 2 IntDisProp_Comp_pct Inge Van den Bergh Bioversity International 2 small blotches Bioversity International/IITA Inge Van den Bergh/Danny Coyne DeadRtNum2_Ct_Rt Danny Coyne HoploNumRtWt2_Comp_Nema IITA OffTypEst_Date_dmy Bioversity International Rhiannon Crichton Initial speck stage: faint, minute, reddish-brown specks on the lower surface of the leaf. 1 Bioversity International Rhiannon Crichton AvgFrtExtLng_M_cm temp44 Tendo Ssali IITA Rachel Chase Bioversity International FrtApxFlwRlt_Est_1to3 HandWt_M_kgPlnt Bioversity International Rhiannon Crichton Bioversity International temp2 Rhiannon Crichton Inge Van den Bergh PStmSplitPres_Est_1or2 Bioversity International IITA-BTI Guillaume Bauchet PulpDiam_M_cm 51-100% of lamina with symptoms 6 temp20 Bioversity International Rhiannon Crichton Bioversity International Rhiannon Crichton PltNtHvstd_Ct_Plnt Danny Coyne RadoNumRtWt2_Comp_Nema IITA Sebastien Carpentier leafAreaIncrease KU Leuven leafAreaIncrease_Comp_cm2 Rhiannon Crichton Bioversity International BlkLfStrkSvrtLfNb_Ct_grd6 PA_EstMale_Txt Noel Madalla Bioversity PStmGrth_M_cm Bioversity International Rhiannon Crichton MatStickT_Meas_0to10 Elizabeth Kakhasa NARO, Uganda Matooke stickiness in hand measurement scale 0-10 Bioversity International Rhiannon Crichton BlkLfStrkLfFrStg6_Date_ymd grainy 5 HvstDst_Comp_PlntHa Bioversity International Rhiannon Crichton high intensity 10 pink-purple 7 YellowPres_Est_0or1 Inge Van den Bergh Bioversity International KU Leuven growthFresh growthFresh_Comp_g plantGrowth_fresh Sebastien Carpentier 1 present BlkLfStrkSvrtLfNb_Ct_grd3 Bioversity International Rhiannon Crichton temp19 Bioversity International Rhiannon Crichton Inge Van den Bergh Bioversity International PlntgDisTim_Comp_d 4 not winged and clasping the pseudostem 2 intermeditae PeelThck_M_mm Bioversity International Rhiannon Crichton 3 brown-black TEtotalDry Sebastien Carpentier transpirationEfficiency_Comp_gml TE_dry KU Leuven Bioversity International Rhiannon Crichton NtHvstDst_Comp_PlntHa 16-33% of lamina with symptoms 4 few internal spots 2 temp35 Bioversity International Rhiannon Crichton 3 ivory 6 other temp22 Bioversity International Rhiannon Crichton Bioversity International Rachel Chase BrctClExtFc_Est_1to16 StdLf_Ct_leaf IITA-Bioversity International Amos Alakonya-Rhiannon Crichton Bioversity International Rhiannon Crichton RtnCrpCyc_Comp_d Bioversity International Rhiannon Crichton BlkLfStrkLfFrStg6_Date_dmy BoiPlantnMeal_Meas_0to10 Boiled plantain Mealiness scale 0-10 CIRAD / UNA, Côte d’Ivoire Hermann Antonin KOUASSI Guillaume Bauchet IITA-BTI DateHarv_Date_ymd BoiPlantnStic_Meas_0to10 Hermann Antonin KOUASSI CIRAD / UNA, Côte d’Ivoire Boiled plantain Stickiness scale 0-10 like fairly 3 NARO, Uganda Elizabeth Kakhasa MatHardT_Meas_0to10 Matooke hardness in hand measurement scale 0-10 34-50% of lamina with symptoms 5 Rachel Chase PMargCol_Esr_1to4 Bioversity International PMargColL_Est_1or2 Bioversity International Rachel Chase Sebastien Carpentier leafFreshMass_Meas_g leaf_fresh massFreshLeaf KU Leuven MgnBhvPtCnlThrdLf_Est_1to4 Bioversity International Rachel Chase 3 6-15% of lamina with symptoms equal to or more than 21 mm 3 pink/pink-purple 6 plant_fresh plantFreshMass_Meas_g KU Leuven Sebastien Carpentier massFreshPlant sticky 10 Initial speck stage: symptoms are first visible to the naked eye as faint, minute (less than 0.25 mm diam), reddish brown specks on the lower surface of the leaf. Specks are often most abundant near the margin of the left side of the leaf, particularly towards the tip. Where leaf spot is severe, specks have been observed on the second leaf of plants that have not yet produced a bunch. Elsewhere they usually appear on the third, fourth, or older leaves. 1 Rachel Chase Bioversity International FrtftPdclLgth_Est_1to3 waterLossPlant waterLossPlant_Meas_ml KU Leuven transpPlant Sebastien Carpentier temp10 Bioversity International Rhiannon Crichton high intensity 10 IITA RotyNumSlWt_CompNema Danny Coyne Rachel Chase PMargWing_Est_1or2 Bioversity International FrtApxFlwRlt_Est_1to4 Bioversity International Rachel Chase KU Leuven areaTotLeaves areaLeaves totLeafArea_Meas_cm2 Sebastien Carpentier NARO, Uganda Matooke surface homogeneity of colour measurement scale 1-10 MatSurfHomColV_Meas_0to10 Elizabeth Kakhasa FrtftPdclLgth_M_mm Rachel Chase Bioversity International revolute (rolling) 1 PS_CompResp_PrefScale Noel Madalla Bioversity pink-purple to red 2 Bioversity Noel Madalla Selection criteria for genotype that was ranked highest by male respondents during preference scoring exercise IITA Danny Coyne PratyCofNumRtWt2_Comp_Nema green 7 10 dont know KU Leuven Sebastien Carpentier massFreshRoot rootFreshMass_Meas_g root_fresh 3-10 egg masses 2 PA_EstResp_Txt Noel Madalla Bioversity Noel Madalla Bioversity PS_CompMale_PrefScale 8 mole rats 5 purple > 100 egg masses 5 Sebastien Carpentier leaf_dry leafDryMass_Meas_g massDryLeaf KU Leuven Bioversity International Rhiannon Crichton PStmHt_M1_cm Bioversity International FrtExtLng_M_mm Rhiannon Crichton BoiPlantnChew_Meas_number of chews Boiled plantain Chewiness by number of chews Hermann Antonin KOUASSI CIRAD / UNA, Côte d’Ivoire yellow 2 Inge Van den Bergh Bioversity International CrmDiscDistTlstSckFW_M_cm Danny Coyne IITA MeloiNumRtWt2_Comp_Nema Rhiannon Crichton PltEst_Date_dmy Bioversity International CmpdTplMnCl_Est_1to6 Rachel Chase Bioversity International 3 hanging at a 45 degree angle fully green 1 CrmIntDiscFW_Est_1to5 Inge Van den Bergh Bioversity International PltFstLfSptd_Ct_leaf Bioversity International Rhiannon Crichton PS_CompFemale_PrefScale Noel Madalla Bioversity IITA PratyNumSlWt_Comp_Nema Danny Coyne curved (sharp curve) 3 Amos Alakonya-Rhiannon Crichton SpotLfNum_Ct_Lf IITA-Bioversity International BlkLfStrkLfFrStg1_Date_ymd Bioversity International Rhiannon Crichton 3 Stage 3 differs from the previous one by its dimensions. The stripe becomes longer, is enlarged and in certain conditions (weak inoculum and unfavourable climatic conditions) can reach 2 or 3 cm. HandAvgWt_M_kgPlnt Bioversity International Rhiannon Crichton Bioversity International Rhiannon Crichton AvgFrtDiam_M_mm trace infections with a few small galls 1 slightly angled 2 sparse blotching 1 KU Leuven lengthSum Sebastien Carpentier totLeafLength_Meas_cm non sticky 0 Inge Van den Bergh Bioversity International PetColpPres_Est_0or1 Bioversity International Rhiannon Crichton DateDeath_Est_dmy KU Leuven topLeafArea_Meas_cm2 area_topView Sebastien Carpentier areaPlantTop straight with erect margins 3 PltPltd_Ct_Plnt Bioversity International Rhiannon Crichton 2 neutral flowers on one to few hands only near the bunch (rest of stalk is bare) Allan Brown IITA GdFrtFll_Est_1to2 PStmHt_M2_cm Bioversity International Rhiannon Crichton temp48 Bioversity International/IITA Inge Van den Bergh/Danny Coyne 2 not revolute (not rolling) Tendo Ssali IITA WvlLvBdLgt_M_µM Inge Van den Bergh/Danny Coyne Bioversity International/IITA temp47 plant_dry plantDryMass_Meas_g KU Leuven massDryPlant Sebastien Carpentier RchsWt_M_kg Bioversity International Rhiannon Crichton Bioversity International BrctImbrcApxMlBd_Est_1to3 Rachel Chase Sebastien Carpentier massDryRoot root_dry KU Leuven rootDryMass_Meas_g 6 Third or mature spot stage: the centre of the spot dries out, becoming light grey or buff-coloured, and further depressed. The spot is surrounded by a narrow, well-defined, dark brown or black border. between the latter and the normal green colour of the leaf, there is often a bright yellow transitional zone. After the leaf has collapsed and withered, spots remain clearly visible because of the light-coloured centre and dark border. HndNbWhlBnch_Ct_hands Rachel Chase Bioversity International NSpotLfProp_Comp_idx IITA-Bioversity International 1 yellow curved in S shape (double curvature) 4 Bioversity International Rhiannon Crichton HndFrtAvgNum_Ct_Frt FngSmshdPlpArm_Est_1to5 IITA-BTI Allan Brown/Guillaume Bauchet (temp.) MatSmoothM_Meas_0to10 Elizabeth Kakhasa NARO, Uganda Matooke smoothness in hand measurement scale 0-10 Tendo Ssali IITA AvgWvlLvBdWght_M_mg Danny Coyne IITA PratyCofNumSlWt_Comp_Nema red 8 Bioversity International Rhiannon Crichton BlkLfStrkSvrtLfNb_Ct_grd4 4 margins overlapping 3 male flowers/bracts above the male bud (rest of stalk is bare) 3 straight in the distal part Bioversity International Rhiannon Crichton FstExtDiSmptFW_Date_dmy MgnBhvPtCnlThrdLf_Est_1to5 Bioversity International Rachel Chase 2 present long (equal to or more than 30 cm) 3 discolouration of between one-third and two-thirds of vascular tissue 4 Christophe Jenny MlRchsTp_Estm_1to2 CIRAD 1 white Tendo Ssali WvlAdltBdWght_M_mg IITA Bioversity International BlotchA_Est_1to5 Rachel Chase PMargClasp_Est_1or2 Bioversity International Rachel Chase Bioversity International BlotchA_Est_1to4 Rachel Chase 4 red-purple 5 bright yellow Tendo Ssali IITA WvlAdltBdLgt_M_µM pseudostemFreshMass_Meas_g massFreshPseudostem pseudostem_fresh KU Leuven Sebastien Carpentier without pigmentation 5 not clasping 2 banana streak virus (BSV) 5 extensive pigmentation 4 with a curve 3 FngWtTtl_Comp_kg Bioversity International Rhiannon Crichton temp9 Bioversity International Rhiannon Crichton other 8 1 cream 2 cream Tendo Ssali IITA TrpNum_Ct_weeviltrap Plantain ontology Banana ontology DamEst_Date_ymd IITA-BTI Guillaume Bauchet WvlAdltHdCps_M_µM Tendo Ssali IITA Bioversity International Rachel Chase BrctBhvBfFll_Est_1to2 1 without any floral relicts Elizabeth Kakhasa Matooke moldability measurement scale 0-10 MatMolT_Meas_0to10 NARO, Uganda Bioversity International Rhiannon Crichton PSuckN_Ct_PSuck 3 large blotches dwarf 1 0 soft temp30 Bioversity International Rhiannon Crichton Bioversity International Rhiannon Crichton FnctLeav_Ct_Leaf BlkLfStrkSvrt_Est_MedLawCpt IITA-Bioversity International rwcPlant_Comp_unitless KU Leuven plant_rwc Sebastien Carpentier rwcPlant KU Leuven Sebastien Carpentier waterLossSystem waterLossSyst_Meas_ml transpSyst Selection criteria for genotype that was ranked third lowest by female respondents during preference scoring exercise Noel Madalla Bioversity Bioversity International Rhiannon Crichton DET_Comp_d 3 watery green 1 no internal symptoms PltDamDesc_Est_txt Bioversity International Rhiannon Crichton leaf spots 1 Bioversity International Rhiannon Crichton FrtGrth_M_mm 9 whitish 25-50 % of roots galled 3 HvstPrp_Comp_PlntNb Bioversity International Rhiannon Crichton 3 intermediate less than or equal to 12 1 DthPrp_Comp_DdPltRt Bioversity International Rhiannon Crichton WvlLv_Ct_weevil Tendo Ssali IITA Bioversity International Rachel Chase FrtNbMdHndBnch_Ct_ft Bioversity International MlBdLngth_M_1to3 Rachel Chase Bioversity International Rhiannon Crichton WSuckN_Ct_WSuck Bioversity International MlBdLngthRt_M_1to3 Rachel Chase 3 discolouration of up to one-third of vascular tissue Noel Madalla Bioversity Selection criteria for genotype that was ranked second lowest by male respondents during preference scoring exercise present 2 2 winged and not clasping the pseudostem 2 lanceolate other 5 Stage 1 appears as a small depigmentation spot whose whitish or yellow colour resembles stage 1 of Yellow Sigatoka disease. These symptoms are not visible in transmitted light and can be observed only on the underside of the leaf. 1 Rachel Chase FstBrctApxShp_Est_1to4 Bioversity International Matooke astringency measurement scale 0-10 Elizabeth Kakhasa MatAstr_Meas_0to10 NARO, Uganda Second-spot stage: the dark brown or black centre of each lesion becomes slightly depressed and water soaked border becomes more pronounced. 5 pointed 1 1 convolute banana Xanthomonas wilt (BXW) 3 medium (0.45 < w/y < 0.55) 2 IITA-Bioversity International BlkLfStrkSvrt_Est_pct other 9 orange red 7 numFuncLeaves_Count_unitless KU Leuven Sebastien Carpentier totalNumFunctionalLeaves numFuncLeaf bad 1 rootToPlantMassRatio_Comp_unitless KU Leuven massDryratioRoot Sebastien Carpentier relativeRoot_dry absent 0 Bioversity International Rhiannon Crichton HndNumMsrd_Ct_Hnd 0 very light yellow YellowPres_Est_1or2 Inge Van den Bergh Bioversity International Bioversity International BnchWt_M_kg Rhiannon Crichton without flower relicts 1 Bioversity International Rhiannon Crichton ShtHvstTime_Comp_d Inge Van den Bergh/Danny Coyne Bioversity International/IITA temp52 Rachel Chase FrtLngth_M_1to5 Bioversity International 6 total discolouration of vascular tissue persistent flower relicts (> 20% of the fruits with relicts) 3 temp18 Bioversity International Rhiannon Crichton Rachel Chase Bioversity International FrtApxPt_Est_1to5 Bioversity International Rachel Chase CmpdTplLbCl_Est_1to16 BlotchCol_Est_1to4 Rachel Chase Bioversity International leafChlorosis_Comp_unitless KU Leuven Sebastien Carpentier leafChlorosis 1 dislike very much contrast between margin and petiole (with a contrasting colour line along) 2 Bioversity International Rhiannon Crichton temp32 Bioversity Selection criteria for genotype that was ranked lowest by male respondents during preference scoring exercise Noel Madalla 1 short (y less than or equal to 20 cm) Rachel Chase Bioversity International MlBdShp_Comp_1to5 green 7 5 > 75 % of roots galled other 5 IITA Danny Coyne PratyNumRtWt2_Comp_Nema less than or equal to 20 cm 1 DmgIdxInn_M_% Tendo Ssali IITA Bioversity International Rhiannon Crichton LfEmRate_Comp_LfMth 6 purple brown lengthily pointed (like plantain) 2 Selection criteria for genotype that was ranked second highest by female respondents during preference scoring exercise Noel Madalla Bioversity HndMltpFrtNum_Ct_Frt Bioversity International Rhiannon Crichton purple-brown 5 4 green yellow AvgWvlAdltHdCps_M_µM Tendo Ssali IITA 4 obtuse and split 6 medium green no visible sign of fusion 1 firm 10 IITA-BTI FlwT_Date_ymd Guillaume Bauchet light green 5 6 Stage 6 is when the centre of the spot dries out, turns clear gray and is surrounded by a well-defined black ring, which is, in turn, surrounded by a bright yellow halo. These spots remain visible after the leaf has dried out because the ring persists. 1 green not winged and not clasping the pseudostem 5 Bioversity International Rhiannon Crichton MltHandWt_M_kgPlnt 2 at an angle Rachel Chase Bioversity International CmpdTplLbCl_Est_1to5 5 obtuse and split Guillaume Bauchet IITA-BTI AvgFrtIntLng_M_cm YlLfPercFW_Comp_pct Bioversity International Rhiannon Crichton 2 red-purple < 1/3 discoloured 3 absent 0 nematodes 7 0 no intensity attribute 4 black-purple skinny (w/y less than or equal to 0.45) 1 Bioversity International Rhiannon Crichton OdStdLfRk_Ct_leaf FrtCvShp_Est_1to6 Rachel Chase Bioversity International 8 dark green base of the style prominent 3 rounded 5 > 1/3 discoloured 5 5 equal to or more than 31 cm moldable 10 4 other Bioversity International Rhiannon Crichton temp14 10 pink/pink-purple 4 only base of the style persists 0 absent corm completely clean, no vascular discolouration 1 1 pointed entity 3 totally fused (more than 50 % of the length of the pedicel) white 1 0 crumbly margins curved inward 4 4 26-30 cm purple 6 0 no intensity 4 spiral (all fruit are attached to a unique crown coiled around the stalk) 4 67-100 % of all leaves turning yellow and necrotic, with leaves hanging down the pseudostem 2 Stage 2 appears as a stripe, generally brown in colour and visible on the underside of the leaf, later the symptom also appears on the upper part of the limb as a stripe, the yellow colour of which resembles the stripe at stage 1 of Yellow Sigatoka. The colour of this stripe will change progressively from brown and later to black on the upper side of the limb, but will retain the brown colour on the underside. 1 bare KU Leuven pseudostemHeightIncrease Sebastien Carpentier pseudostemHeightIncrease_Comp_cm2 10 other 1 less than or equal to 2 m 3 equal to or more than 31 cm Boiled plantain Moistness scale 0-10 Hermann Antonin KOUASSI BoiPlantnMois_Meas_0to10 CIRAD / UNA, Côte d’Ivoire 7 blue 2 one yellow finger 2.1-2.9 m 2 3 34-66 % of older leaves turning yellow, with some hanging down the pseudostem Sebastien Carpentier widthPerLeaf singleLeafWidth_Meas_cm widthSingleLeaf KU Leuven like extremely 5 Inge Van den Bergh Bioversity International CormDiscol_Est_1to6 plant dead, with brown leaves hanging down the pseudostem 5 blunt-tipped (plateau at tip) 3 Bioversity International FrtWt_M_gFruit Rhiannon Crichton rounded 5 1 absent Sebastien Carpentier root_rwc KU Leuven rwcRoot_Comp_unitless rwcRoot few flower relicts (< 20% of the fruits with relicts) 2 Inge Van den Bergh/Danny Coyne temp49 Bioversity International/IITA 3 equal to or more than 17 1 clasping cream 2 yellow 3 First-spot stage: the streaks broaden and become more or less fusiform or elliptical in outline and a water soaked border appears around each lesion. 4 3 low shouldered (x/y more than or equal to 0.30) 2 medium (20 cm < y < 30 cm) not winged 2 4 banana bunchy top (BBTD) other 8 2 dark brown 50-75 % of roots galled 4 4 horizontal or supra-horizontal 1 less than or equal to 15 cm 5 erect 1 adult male 1 winged and undulating 4 like very much First-spot stage: the streak broadens and becomes more or less fusiform and elliptical in outline and similar to the first-spot stage of Sigatoka (Leach 1946). The transition from streak to spot is further characterised by the development of a light brown, water-soaked border around the spot. This water-soaked effect is especially clear in the early morning, when dew is still present on the leaf, or after rain. 4 Stage 5 is when the elliptical spot is totally black and has spread to the underside of the limb. It is surrounded by a yellow halo with the centre beginning to flatten out. 5 yellow 2 5 medium intensity orange red 10 Bioversity International Inge Van den Bergh PStmSplitPres_Est_0or1 Bioversity International Rhiannon Crichton temp17 2 16-20 cm 5 medium intensity 1 pointed green 1 no pigmentation 1 acronym obtuse 3 4 strongly bottle-necked (wider under tip than number 2) winged 1 Bioversity International/IITA Inge Van den Bergh/Danny Coyne temp46 sparse blotching (<20%) 2 highly imbricate 3 fusarium wilt 2 3 juvenile persistent style 2 5 light green Second-streak stage: the streaks may elongate slightly, but the most notable change is that in colour from reddish brown to dark brown or almost black, sometimes with a purplish tinge. The streak is now clearly visible on the upper surface of the leaf. The distribution of streaks at this stage of development varies in the same way as mentioned above. When streaks are very numerous, and more or less evenly distributed, the entire leaf blackens. 3 1 1-2 egg masses 3 red horizontal 4 less than 1% of lamina with symptoms, only streaks and/or up to 10 spots 1 0 no egg masses present 1 2 < 25 % of roots galled 6 orange intermediate 3 5 discolouration greater than two-thirds of vascular tissue firm 5 other 4 whitish 9 Third or mature spot stage: the centres of the lesions dry out becoming light grey or buff coloured and a bright yellow transitional zone appears between them and the normal green colour of the leaf. The lesions remain clearly visible after the leaf has collapsed or withered because of their light coloured centre and dark border. 6 not dwarf 2 2 brown not parthenocarpic 2 green yellow 4 4 curved (sharp curve) 0-33 % of older banana leaves turning yellow 2 4 obtuse 0 lumpy 1 less than or equal to 10 mm entire inner rhizome discoloured 6 4 grey 1 parthenocarpic 4 egg 8 dark green no intensity 0 cream 1 high intensity 10 moderate blotching (20-50%) 3 margins erect 2 3 winged and clasping the pseudostem asymmetrical 3 high intensity 10 like a top 1 partially fused (up to 50 % of the length of the pedicel) 2 FstYngLfsptd_Est_dmy IITA-Bioversity International Amos Alakonya-Rhiannon Crichton 3 21-25 cm 2 wide with erect margins Other 5 leaves/month Finger peel thickness - measurement Cut a finger in half, crosswise at its widest point, and on the cross-section measure the width of the peel, using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Length [x at maturity] Length of the internal arc of a finger ** subsume Fruit length BD colour chart B - 1996 The relative water content in a root Content Root relative water content rwcRoot nematodes/unit of fresh root weight Appearance in relation to the presence or absence of neutral, male and/or hermaphrodite flowers Male rachis appearance Appearance of flowers/bracts on the male rachis Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked third lowest in preference ranking exercise Focus group discussion for third lowest ranked traits with female respondents Number of leaves with a black leaf streak disease severity grade of 3 The number of leaves on a plant with a black leaf streak disease severity grade of 3. Number of leaves with black leaf streak disease severity grade 3 numLeaves Number The number of all leaves (including dried/dead/wilted/yellow) in a shoot system Leaf number Water loss rate - computation Measurement of system water loss between two timepoints (refer to experimental metadata) Volume The amount of water loss through the plant over time Plant water loss waterLossPlant Matooke smoothness geometrical textural attribute relating to lack of presence of particles in a product MatSmoothM Matooke Smoothness in mouth Angle to the vertical Angle between the axis of the bunch and the vertical Bunch position Mechanical textural attribute relating to the force required to achieve a given deformation, penetration, or breakage of a product. Matooke firmness Firmness in mouth Matooke MatFirmM Count how many hands are used to obtain a measurement. Number of hands measured - counting Matooke MatMoistM Moisture in mouth perception of moisture content of a food by the tactile receptors in the mouth and also in relation to the lubricating properties of the product Matooke moisture Visual estimation of bunch quality, for Early Evaluation Trial stage only. Bunch quality - estimation Calculate as: the Total finger weight, divided by the Number of hands in bunch, e.g. 46.8 / 16 = 2.9. Average hand weight - all - computation mL Colour The color of the unsmashed pulp of a mature fruit Fruit unsmashed pulp colour The relative water content in the whole plant Plant relative water content Content rwcPlant Number of nematodes (Pratylenchus goodeyi) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Put a part of the sample between thumb and index fingers and using tapping motions, evaluate the amount of product adhering on them Stickiness in hand method Average potential annual yield The average potential annual yield, assuming that all plants are harvested, from the number of plants planted. potannyield Average potential annual yield Number of peeper suckers numpeepersuckers Number of peeper suckers The number of peeper suckers (suckers less than 15 cm tall) in the mat. moldability scale 0-10 Description of traits considered important in selection of second best genotype by respondents Genotype selection traits Selection criteria for genotype ranked second highest by respondents The date and time of the start of data collection on a plant. Start date and time of data collection Date and time of start of data collection datestarttimedatacoll weevil Plant circumference at collar - measurement Measure the circumference of the pseudostem of the plant at the collar, or from the pseudostem base at the ground if the collar is not visible. leafDryMass The dry weight of a leaf Dry weight Leaf dry weight Number of fingers in bunch - counting Count how many fingers are in the bunch. The presence/absence of a splitting pseudostem base, caused by Fusarium wilt. SP Presence of splitting pseudostem base caused by Fusarium wilt Presence/absence of splitting Visually observe the pigmentation of the outer surface of the cigar leaf of a developed sucker or on another plant of the same accession. [x Observation should be made before shooting, on a developed sucker or on another plant of the same accession.] Pigmentation of outer surface of cigar leaf - estimation Extent of internal discolouration in corm of plant caused by Fusarium wilt The extent of discolouration in the corm of the plant, caused by Fusarium wilt. Extent of discolouration % Count how many hands are in the bunch. Number of hands in bunch - counting Number of maiden suckers Number of maiden suckers The number of maiden suckers (fully grown suckers with foliage leaves) in the mat. nummaidensuckers pulp colour Count how many functional leaves (leaves that have 50% or more of their surface as green, healthy, photosynthetic tissue) are on the plant. Number of functional leaves - counting The number of leaves on a plant with a black leaf streak disease severity grade of 2. Number of leaves with black leaf streak disease severity grade 2 Number of leaves with a black leaf streak disease severity grade of 2 Matooke moldability Matooke Moldability in hand MatMolT mechanical textural attribute relating to the degree to which a substance can be deformed before it breaks Damage to lower inner corm weevils - measurement Cut a transverse cross section of the corm at 10 cm below the collar (lower cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. No. per 10g Gauhls modification of Stovers severity scoring system (YYYY) Water loss - measurement Weighing of water loss though plant between timepoints (refer to experimental metadata) How many banana weevil larvae used to obtain a measurement. Number of banana weevil larvae measured Number of banana weevil larvae measured Fruit pedicel length Length of the fruit pedicel from rachis to the beginning of the pulp Length Visually observe the fruit apex point and choose the option that is most similar, from the 5 photos of descriptor 6.7.6 in the reference material. Fruit apex point - estimation g Measurement under microscope Weevil larvae body length - measurement The date the bunch is harvested from the plant. dateharvest Date of harvest Date of harvest Record a description of the damage to the plant, e.g. leaves ripped, pseudostem snapped, plant toppled, etc. Damage description - estimation Off-type description - estimation Record a description of what made the plant recogniseable as an off-type. numfingersouterwhorl2nddistalhand The number of fingers in the outer whorl of the second-most distal hand Number of fingers in outer whorl Number of fingers in outer whorl of 2nd-most distal hand Number of Radopholus similis per soil sample Population density Number of Radopholus similis in soil Ratio of non green to green plant pixels Leaf chlorosis - computation Average finger circumference - computation Calculate as: the sum of the Finger circumference measurements, divided by the number of those measurements, e.g. (7.0 + 8.0 + 9.0 + 10.0 + 7.0 + 9.5) / 6 = 8.4. Number of leaves with black leaf streak disease severity grade 5 The number of leaves on a plant with a black leaf streak disease severity grade of 5. Number of leaves with a black leaf streak disease severity grade of 5 Blotches colour - estimation Observe visually the colour of the blotches on the upper leaf sheath. Count how many fingers are in the outer whorl of the second-most distal hand. Number of fingers in outer whorl of 2nd-most distal hand - counting Researchers calculate the preference score by respondents Preference score individual respondents End date and time of data collection Date and time of end of data collection The date and time of the end of data collection on a plant. dateendtimedatacoll Place the sample in the mouth, chew it at the rate of one chewing per second and assess the number of chews before swallowing. Chewiness Sensory Texture method mL / cm? Individual male respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. Preference scoring scale male respondents Visual appearance - estimation Subjective visual assessment of smashed and unsmashed fruit pulp Ex: g/100 mL juice???? The amount of damage to the inner corm (central cylinder) upper cross-section, assessed by the percentage of tunnels caused by weevils. Percentage damage to upper cross-section inner corm caused by weevils Damage percent X-upper-inner Population density Number of Helicotylenchus multicinctus in soil Number of Helicotylenchus multicinctus per soil sample Visually observe the bract imbrication of the apex of the male bud and choose the option that is most similar, from the 3 photos of descriptor 6.5.3 in the reference material. Bract imbrication at apex of male bud - estimation Growth - computation Leaf area increase between timepoints (refer to experimental metadata) per number of new leaves formed between timepoints (refer to experimental metadata) Count from 100 ml of soil sample Number of nematodes (Radopholus similis ) per unit fresh root weight - method hand Calculate as: the time elapsed between the Date of planting and the Date of shooting. E.g. 24/09/2014 - 24/01/2014 = 243 days. Time from planting to shooting - computation Leaf area ratio - computation Computation of top leaf area with total (loose) leaf area plants dead/plants planted Percentage of plants with petiole collapse caused by Fusarium wilt Percentage of plants with petiole collapse, caused by Fusarium wilt. Combined percentage with petiole collapse Cut the hands off from the rachis and weigh all the hands together, using scales. Total finger weight - measurement cm Measure the circumference of the pseudostem of the plant at 75 cm from the collar, or from the pseudostem base at the ground if the collar is not visible, using a tape measure. Plant circumference 75 cm - measurement numfingers2ndhand The number of fingers in the second hand. Number of fingers in 2nd hand Number of fingers blotch pigmentation scale (1 to 4) The smoothness-mouth feel of the smashed pulp of a mature fruit Fruit smashed pulp smoothness-mouth feel Smoothness-mouth feel The date the last bracts fall from the most distal hand (closest to the male bud) to display the female flowers. Date of flowering dateflower Date of flowering fruit curve shape (scale 1 to 5) fruit length scale (1 to 5) Calculate as: the time elapsed between the Date of planting and the Date of first youngest leaf spotted, e.g. 24/06/2014 - 24/01/2014 = 151 days. Time from planting to first youngest spotted leaf - computation Nosal smell - estimation Nosal smell assessment of smashed and unsmashed fruit pulp aromas harvest2harvest Ratoon crop cycle The time elapsed from the harvest of the bunch of the plant from the preceding crop cycle to the harvest of the bunch of the plant from the current crop cycle. Ratoon crop cycle Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Hoplolaimus spp.) per unit fresh root weight -method µM Calculate as: the Plant density multiplied by the Death proportion, e.g. 1,667 * 0.17 = 283. Death density - computation Visually observe the male rachis appearance and choose the option that is most similar. Male rachis appearance - estimation wing presence/absence Mouth feel assessment of smashed and unsmashed fruit pulp Smoothness-Mouth feel - estimation The presence/absence of petiole collapse, caused by Fusarium wilt. PC Presence/absence of collapse Presence of petiole collapse caused by Fusarium wilt Average body weight Banana weevil larvae average body weight The average weight of a banana weevil larvae body. Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Record on the last developed leaf at [x flowering stage] [shooting]. Colour line along edge of petiole margin - estimation Population density Number of Hoplolaimus spp. in roots Number of Hoplolaimus spp. per fresh root weight Date of flowering - estimation Record the date of event. m2 BD colour chart A - 2016 unitless Margins clasping or not clasping Behaviour of the petiole margins - winged or not winged. the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Petiole margins clasping Presence of changes in new leaves caused by Fusarium wilt - estimation Visual assessment. Number of Pratylenchus spp. in soil Number of Pratylenchus spp. per soil sample Population density Population density Number of Pratylenchus goodeyi in roots Number of Pratylenchus goodeyi per fresh root weight plantcircumf100cm Plant circumference 100 cm from the collar The circumference of the pseudostem of the plant at 100 cm from the collar. Circumference 1 m above the ground Presence of petiole collapse caused by Fusarium wilt - estimation Visual assessment. The number of fingers in the second-most distal hand Number of fingers numfingers2nddistalhand Number of fingers in 2nd-most distal hand stickiness scale 0-10 Average lateral diameter of the pulp avepulpdiam The average lateral (side to side) diameter of the pulp of a fruit. Average fruit pulp lateral diameter Male bud shape - computation Calculate the ratio w/y, i.e. the broadest width of the male bud to the total length of the male bud. Do not measure the dimensions along the bud but rather on a projection/outline of the bud (e.g. trace the outline of the bud on paper) and use ruler or measuring tape. Pest and/or disease assessment and/or sample collection options - estimation Record the pest and/or disease assessment and/or sample collection taking place at the time of data collection. Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. From the functional roots, randomly select five primary roots that are at least 10 cm, and reduce their length to 10 cm. Where root knot nematode galls are present, determine the extent of the galling using the scale. Nematode root knot galling extent - estimation Fresh weight - measurement Weighing Bract behaviour before falling Behaviour of the last lifted bract - revolute or not revolute Behaviour before falling, whether revolute or not revolute Leaf yellowing and wilting caused by Fusarium wilt rating A rating of the extent of leaf yellowing and wilting, caused by Fusarium wilt. Rating of external symptoms of Fusarium wilt in the field Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Pratylenchus coffeae) per unit fresh root weight -method Number of hands on whole bunch - counting On a bunch with mostly hands of > 10 fingers, a possible ultimate hand with 1-5 (rather smaller) fingers should not be counted. Calculate as: the Number of plants dead, divided by the Number of plants planted, e.g. 4 / 24 = 0.17. Death proportion - computation plantdens The number of plants that are planted, in a defined area. Combined density of plants planted Plant density Colour of the blotches on the upper leaf sheath of the petiole base. Blotches colour at the petiole base Main colour Stunting expressed as height growth per new formed leaf Pseudostem height increase per leaf formed pseudostemHeightIncreasePerFormedLeaf Growth Number of dead roots - counting Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. Count the number of dead roots. The weight of the rachis. Weight rachisweight Rachis weight Population density Number of Meloidogyne spp. per fresh root weight Number of Meloidogyne spp. in roots Record the date of event. Date of planting - estimation Average finger external length - computation Calculate as: the sum of the Finger external length measurements, divided by the number of those measurements, e.g. (30 + 34 + 32 + 33 + 33 + 31) / 6 = 32.1. The average length of the fingers measured along the internal (ventral) arc, excluding the pedicel and the fruit tip. Average internal length avefingerintlength Average finger internal length kg Fruit shape of curve - estimation Visually observe the shape of the curve of the fruit and choose the option that is most similar, from the 5 photos of descriptor 6.7.4. in the reference material. Observe the inner fruit in the middle of the mid-hand of the bunch. In case of an asymmetric bunch that has straight and curved fruits, please indicate it in the note section and score only the fruit on the upper side of the bunch. Weighing Fresh weight - measurement Fusion Degree of fusion of the pedicels before they join the rachis Fusion of pedicels Count the number of leaves (functional only > 50% estimated green area) Leaf number - counting Record the date of event. Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 - estimation functional roots Genotype selection traits Selection criteria for genotype ranked second lowest by respondents Description of traits considered important in selection of second least genotype by respondents Single leaf width Width The width of a leaf singleLeafWidth Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked highest in preference ranking exercise Focus group discussion for top traits with male respondents Calculate as: the sum of the Finger internal length measurements, divided by the number of those measurements, e.g. (27 + 31 + 29 + 30 + 30 + 28) / 6 = 29.2. Average finger internal length - computation Date of data collection - estimation Record the date of event. Selection traits (text) Number of weevil larvae - counting Visual counting method Percentage of plants with external disease symptoms caused by Fusarium wilt Combined percentage with external symptoms of Fusarium wilt The percentage of plants with any or multiple of the external disease symptoms - yellowing leaves, splitting of pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. The longest diagonal of a fitted ellips Leaf length - measurement bunchmaturity Stage of maturity at harvest The stage of maturity of the bunch at harvest. Bunch maturity stage at harvest Ratio of projected leaf area to the actual leaf area, measure for the compactness of the plant crown leafAreaRatio Ratio Leaf area to plant area ratio Lobe colour at tip of the tepal Main colour of the tip of the compound tepal (lobe) Lobe colour of tip of compound tepal MatSweet Matooke sweetness Matooke Sweetness basic taste produced by dilute aqueous solutions of natural or artificial substances such as sucrose Firmness scale 0-10 The dry weight of the whole plant. Plant dry weight plantDryMass Dry weight 80?C, for 14 days, or longer untill dry Dry weight - measurement The number of fingers in a hand. numfingershand Number of fingers Number of fingers in hand Combined proportion of plants not harvested Non-harvest proportion The proportion of plants that are alive but not harvested (e.g. due to slow or poor development), from the number of plants planted. nonharvprop Weight multhandweight Multiple hand weight The weight of multiple hands. Detach the outermost sheath from the pseudostem (the sheath should not be too dry). Record the overall impression of colour of the exposed surface of the underlying pseudostem. Note that this main colour should cover more than 75% of the underlying pseudostem surface. Use colour chart A and observe out of direct sunlight. Pseudostem colour - estimation Percentage of functional roots The percentage of all roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant that are functional. Percentage of functional roots Smoothness in mouth method Put a part of the sample in mouth, chew it and after 5 chews, evaluate between tongue and palate the number and the size of the particles. Average hand weight The average weight of the hands. Average weight avehandweight Perception of the amount of water absorbed or released by the product Boiled plantain Moistness Boiled plantain Moistness BoiPlantnMois plant Dwarfism - estimation Visual estimation of dwarfism, for Early Evaluation Trial stage only. Record the apparent cause of the damage to the plant, e.g. strong winds, weevil damage, etc. Cause of damage - estimation Measurement under microscope Adult weevil head capsule - measurement day leavesFormedPerPseudostemGrowth Stunting expressed as number of leaves per height growth Leaves formed per pseudostem height increase Formation Finger pendulance quality Overall fruit pendulance quality pendulantfruit The overall estimation fruit pendulance on a bunch Put a part of the sample in the mouth, chew and evaluate the quantity of water within the sample. Moisture in mouth method Distance of internal discolouration in pseudostem of tallest sucker, caused by Fusarium wilt For the tallest sucker, the complete corm should be removed from the soil, the roots cut off and excess soil removed. Cross-sections of the corm should be cut (using a guillotine or other suitable device) to obtain five slices of equal thickness. The upper surface of each cut section should be examined and visually evaluate the extent of the vascular discolouration. Distance of internal discolouration in pseudostem of tallest sucker caused by Fusarium wilt bract apex scale (1 to 4) Measure the length of a finger that is at the ready to eat stage, along the external (dorsal) arc, excluding the pedicel and the fruit tip, using a tape measure. Associate the data with the Hand rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger external length - measurement Electronic weighing method Adult weevil body - measurement CNL Presence/absence of changes The presence/absence of changes in new leaves - irregular pale margins, narrowing of lamina, burning plus ripping of lamina, lamina becoming more erect, caused by Fusarium wilt. Presence of changes in new leaves caused by Fusarium wilt Pumpkin-like aroma Matooke MatAroPumpA Aroma of pumpkin. Possibly, precise if the taste is like boiled pumpkin or fried... Matooke aroma pumpkin The shortest diagonal of a fitted ellips Leaf width - measurement Annual crop cycle proportion - computation Calculate as: the length of a year (e.g. 365 days) divided by the Plant crop cycle or the Ratoon crop cycle, e.g. 365 / 334 = 1.09. astringency scale 0-10 Put a part of the product and by retro-olfaction evaluate the presence and the intensity of pumpkin-like aroma Pumpkin-like Aroma method Focus group discussion for lowest ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked lowest in preference ranking exercise Fruit unsmashed pulp smoothness-mouth feel The smoothness-mouth feel of the unsmashed pulp of a mature fruit Smoothness-mouth feel A rating of the extent of rhizome (corm) discolouration, caused by Fusarium wilt. Discolouration caused by Fusarium wilt rating Rating of internal symptoms of Fusarium wilt in the greenhouse % Focus group discussion for third lowest ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked third lowest in preference ranking exercise Time from planting to death - computation Calculate as: the time elapsed between the Date of planting and the Date of death, e.g. 24/05/2014 - 24/01/2014 = 120 days. Bunch weight - measurement Harvest the bunch by cutting the peduncle 10 cm above the first ridge above the most proximal, hand and immediately below the most distal hand and weigh the bunch (including the rachis), using scales. Finger lateral diameter The lateral (side to side) diameter of a finger. fingerlatdiam Lateral diameter Visually observe the colour of the external face of the first unlifted bract. Use colour chart A and observe out of direct sunlight. Colour of bract external face - estimation Date of tagged cigar leaf unrolling at Bruns stage B Date of tagging at Bruns stage B of unrolling The date a tagged cigar leaf is at Bruns stage B of unrolling. finger Finger external length fingerextlength External length The length of a finger measured along the external (dorsal) arc, excluding the pedicel and the fruit tip. Male bud shape Shape of male bud Shape Presence of yellowing leaves caused by Fusarium wilt - estimation Visual assessment. smoothness scale 0-10 Margin behaviour on petiole canal of third leaf Behaviour Margin behaviour of the petiole canal of the third leaf before bunch. The amount of water loss in the system normalized by the leaf area Volume waterLossSystPerLeafArea System water loss per leaf area waterLossDay Rate The average daily water loss Daily rate of water loss deathprop The proportion of plants that are dead, from the number of plants planted. Death proportion Combined proportion of plants that are dead Percentage damage to lower cross-section outer corm caused by weevils The amount of damage to the outer corm (cortex) lower cross-section, assessed by percentage of tunnels caused by weevils. Damage percent X-lower-outer Rachis weight - computation Calculate as: Bunch weight minus the sum of the Hand weight measurements coming from the count of number of hands (equivalent to Number of hands in bunch), e.g. 50 - (2.4 + 3.6 + 3.2 + 4.1 + 4.0 + 3.9 + 3.6 + 3.3 + 3.3 + 2.9 + 2.9 + 2.8) = 10.0. Banana weevil larvae average body length The average length of a weevil larvae body, from the head to the tip of the abdomen. Average body length Shape of a fruit (finger) in the middle of the bunch **subsume Shape of the longitudinal curvature Fruit shape of curve 1 to 5 acceptability score Number used to obtain a measurement The number of hands used to obtain a measurement. numhandsmeas Number of hands measured Internal length fingerintlength Finger internal length The length of a finger measured along the internal (ventral) arc, excluding the pedicel and the fruit tip. bract imbrication scale (1 to 3) Root necrosis caused by nematodes - estimation Select at random five functional primary roots, at least 10 cm long. Reduce the length of the five selected roots to 10 cm and slice through the roots length-wise. Evaluate one half of each of the five roots for the percentage of root cortex showing necrosis. The maximum root necrosis per root half can be 20 %, giving a maximum root necrosis of 100 % for the five halves together. Mentally determine the necrosis of the individual roots (1 to 5) and sum these together to get the total root necrosis of the sample. dd/mm/yyyy Total length of all leaves totLeafLength The length of a leaf Length Plant height - petiole of two youngest leaves - measurement Measure the distance from the collar, or from the pseudostem base at the ground if the collar is not visible, to the intersection of the petioles of the two youngest leaves (leaf ranks 1 and 2), using a measuring pole or sliding ruler. plantannyield Annual yield Plant annual yield The bunch weight produced by the plant over the course of the crop cycle, averaged across one year. Population density Number of Meloidogyne spp. in soil Number of Meloidogyne spp. per soil sample Put a part of the product and by retro-olfaction evaluate the presence and the intensity of grassy-like aroma Grassy-like Aroma method Number of nematodes in soil Population density Nematode population density by species in soil Number of nematodes (Radopholus similis ) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Leaf relative water content Content The relative water content in a leaf rwcLeaf leaf blotch color scale (1 to 5) Count from 100 ml of soil sample Number of nematodes (Helicotylenchus multicinctus) per unit fresh root weight - method Visual estimation of suckering, for Early Evaluation Trial stage only. Sucker quality - estimation Visually observe the colour of the internal face of the first unlifted bract. Use colour chart A and observe out of direct sunlight. Colour of bract internal face - estimation Distance of internal discolouration in pseudostem of plant caused by Fusarium wilt For the plant, the complete corm should be removed from the soil, the roots cut off and excess soil removed. Cross-sections of the corm should be cut (using a guillotine or other suitable device) to obtain five slices of equal thickness. The upper surface of each cut section should be examined and visually evaluate the extent of the vascular discolouration. None Index of black leaf streak disease severity mg Rank of marked youngest leaf - counting Mark the youngest completely unrolled leaf and record its rank, which is always 1. Associate the data with the Date of data collection. Calculate as: the sum of the Number of fingers in hand, divided by the Number of hands in bunch, e.g. (10 + 10 + 11 + 10 + 10 + 10 + 12 + 12 + 12 + 11 +12 + 12 + 10 + 10 + 10 + 11) / 16 = 10.8. Average number of fingers in hand - hands - computation Combined density of plants harvested harvdens The number of plants that are harvested, in a defined area. Harvest density BD colour chart A - 1996 Count from 100 ml of soil sample Number of nematodes (Pratylenchus coffeae) per unit fresh root weight - method Number of leaves with a black leaf streak disease severity grade of 4 Number of leaves with black leaf streak disease severity grade 4 The number of leaves on a plant with a black leaf streak disease severity grade of 4. Ratio - computation Ratio of root dry mass versus plant dry mass Hardness in hand method Take a part of the sample between fingers and evaluate how hard the sample is Measure the length (y) of a projection/outline of the male bud on paper, with a measuring tape or ruler. Male bud length - measurement Number of Pratylenchus coffeae in soil Population density Number of Pratylenchus coffeae per soil sample Record the date and time of event. Start date and time of data collection - estimation Count from 100 ml of soil sample Number of nematodes (Rotylenchulus spp.) per unit fresh root weight - method Dry weight Pseudostem dry weight The dry weight of the pseudostem pseudostemDryMass The percentage of the root cortex of five functional roots approximately 10 cm long that is necrotic due to nematode damage. Percentage necrotic Root necrosis caused by nematodes Calculate as: the sum of the Finger lateral diameter measurements, divided by the number of those measurements, e.g. (3.5 + 5.2 + 4.1 + 5 + 6 + 6.8) / 6 = 5.1. Average finger lateral diameter - computation BD colour chart B - 2016 Top view image of loose leaves Leaf area - measurement Number of leaves with black leaf streak disease severity grade 0 Number of leaves with a black leaf streak disease severity grade of 0 The number of leaves on a plant with a black leaf streak disease severity grade of 0. Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked third highest in preference ranking exercise Focus group discussion for third ranked traits with female respondents Apex point Description of fruit apex Fruit apex point A rate that expresses the number of leaves that have emerged from the pseudostem in a week. LER Weekly leaf emission rate Weekly leaf emission rate Banana weevil larvae body length Body length The length of a weevil larvae body, from the weevil larvae head to the tip of the abdomen. moisture scale 0-10 Growth - computation Pseudostem height difference between timepoints (refer to experimental metadata) Count only fully developed fruit. If there is an even number of hands, there will be two middle hands. Count the middle hand that developed first. Number of fruits on mid-hand of the bunch - counting A root weight trait which is the fresh weight of a root Fresh weight rootFreshMass Root fresh weight The distance of discolouration in the pseudostem of the plant, caused by Fusarium wilt. Distance of internal discolouration in pseudostem of plant caused by Fusarium wilt Distance of discolouration Calculate as: the Rank of previously marked leaf at one point in time, minus 1 (equivalent to the Rank of marked youngest leaf), divided by the time elapsed between the two Date of data collection events when the marked leaf was recorded as 1) the Rank of marked youngest leaf and 2) the Rank of previously marked leaf, e.g. (4 - 1) / 1 month = LER of 3 leaves/month during a particular time period. Monthly leaf emission rate - computation Number of Pratylenchus goodeyi in soil Number of Pratylenchus goodeyi per soil sample Population density Growth - computation Pseudostem height difference between timepoints (refer to experimental metadata) per number of new leaves formed between timepoints (refer to experimental metadata) Percentage of plants with changes in new leaves, caused by Fusarium wilt. Percentage of plants with changes in new leaves caused by Fusarium wilt Combined percentage with changes in new leaves Pseudostem height - estimation Recorded from the base of the pseudostem to the emerging point of the peduncle. Matooke MatSurfHomColV Surface color homogeneity Uniformity of color of the surface of the sample Matooke homogeneity of surface colour Stickiness scale 0-10 Date of planting Date of planting The date of planting the plant. dateplant Number of plants dead Combined number of plants that are dead numdead The number of plants that are dead. Selection criteria for genotype ranked third highest by respondents Genotype selection traits Description of traits considered important in selection of third best genotype by respondents Ratio - computation Ratio of pseudostem dry mass versus plant dry mass Nematode multiplication from single root inoculation - computation Calculation from count pseudostemFreshMass Fresh weight Pseudostem fresh weight A pseudostem weight trait which is the fresh weight of a pseudostem Calculate as: ((((Number of leaves with black leaf streak disease severity grade 0, multiplied by 0), plus (Number of leaves with black leaf streak disease severity grade 1, multiplied by 1), plus (Number of leaves with black leaf streak disease severity grade 2, multiplied by 2), plus (Number of leaves with black leaf streak disease severity grade 3, multiplied by 3), plus (Number of leaves with black leaf streak disease severity grade 4, multiplied by 4), plus (Number of leaves with black leaf streak disease severity grade 5, multiplied by 5), plus (Number of leaves with black leaf streak disease severity grade 6, multiplied by 6)), divided by the number of grades in the scale minus 1 (i.e. 7-1)), divided by the Number of standing leaves), multiplied by 100. For example, ((((3 * 0) + (1 * 1) + (1 * 2) + (3 * 3) + (5 * 4) + (0 * 5) + (0 * 6)) / 6) / 13) * 100 = ((32 / 6) / 13) * 100 = 41. Black leaf streak disease severity index - computation Leaf to whole plant mass ratio leafToPlantMassRatio_dry Measure for the biomass allocation: dry mass of leaves versus whole plant dry mass ratio Ratio Preference number scale plants harvested/plants planted scale_of Date of damage datedamage Date of damage The date damage to the plant is first observed. Record the date of event. Date of weevil damage to corm assessment - estimation leaf rank Count the number of leaves Leaf number - counting Sourness method Put a part of the sample in the mouth and evaluate the intensity of the sourness Average number of banana weevil larvae Average number of banana weevil larvae per trap The average of how many banana weevil larvae caught per trap. Number of leaves with black leaf streak disease severity grade 6 The number of leaves on a plant with a black leaf streak disease severity grade of 6. Number of leaves with a black leaf streak disease severity grade of 6 Projected top area Area topLeafArea The projected leaf area of a plant (photosynthetic surface) kg Measure the lateral diameter of a finger from the left to the right side (not from the ventral to the dorsal side), at the widest point, using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger lateral diameter - measurement Ratio Pseudostem to whole plant mass ratio pseudostemToPlantMassRatio_dry Measure for the biomass allocation: dry mass of pseudostem versus whole plant dry mass ratio Count how many fingers are in multiple hands. Associate the data with the Hand rank and the Number of hands measured. The recommendation is to collect this data from the second and second-most distal hands. Multiple number of fingers in hand - counting Total finger weight - computation Calculate as: the Bunch weight, minus the Rachis weight, e.g. 50 - 10 = 40. How many How many banana weevil traps used. Number of banana weevil traps used The average of how many adult banana weevils caught per trap. Average number of banana weevil adults Average number of banana weevil adults per trap Number of functional leaves tallsuckererfunctleaves The number of leaves on the tallest sucker that have 50 % or more of the leaf surface area as green, healthy, photosynthetic tissue. Tallest sucker number of functional leaves Winged petiole margin - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards. [x Observe at flowering time.] Petiole margin colour Colour Main colour of the petiole margin. Where the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Cause of damage The apparent cause of damage to the plant. Cause of damage causedamage The texture of the smashed pulp of a mature fruit Fruit smashed pulp texture Texture Record the date of event. Date of first youngest leaf spotted - estimation Visual assessment. Rating of external symptoms of Fusarium wilt in the field - estimation Presence absence 0 Number of fingers in bunch - estimation Calculate as: the sum of the Number of fingers in 3rd hand and the Number of fingers in 2nd-most distal hand, divided by 2, and multiplied by the Number of hands in bunch, e.g. (16 + 12) / 2 * 8 = 112. Calculate as: the Rank of previously marked leaf at one point in time, minus 1 (equivalent to the Rank of marked youngest leaf), divided by the time elapsed between the two Date of data collection events when the marked leaf was recorded as 1) the Rank of marked youngest leaf and 2) the Rank of previously marked leaf, e.g. (2 - 1) / 1 week = LER of 1 leaves/week during a particular time period. Weekly leaf emission rate - computation plant2harvest Plant crop cycle Time from planting to harvest The time elapsed from planting to the harvest of the bunch. The third leaf (Leaf III) is counted from the last leaf produced before [x bunch emergence] [shooting]. Cut the petiole halfway between the pseudostem and the leaf blade and examine the cross section. [x Observe at flowering time]. Margin behaviour on petiole canal of third leaf - estimation Visual assessment. Rating of internal symptoms of Fusarium wilt in the greenhouse - estimation rootDryMass Dry weight The dry weight of the root system Root dry weight bud shape scale (1 to 5) Number of all roots - computation Calculate as: the Number of dead roots plus the Number of functional roots, e.g. 8 + 12 = 20 roots. Percentage of plants with external disease symptoms caused by Fusarium wilt - computation Calculate as: the sum of the number of plants with any or multiple of the external disease symptoms caused by Fusarium wilt - yellowing leaves, splitting of pseudostem base, changes in new leaves, petiole collapse - divided by the Number of plants planted * 100, e.g. 112 / 300 * 100 = 37%. The proportion of the crop cycle that takes place in one year. anncropcycprop Proportion of crop cycle that takes place in one year Annual crop cycle proportion Fruit pedicel length - exact value - measurement Measure from the scar on the rachis until the beginning of the fruit. Record on the inner fruit in the middle of the mid-hand of the bunch. Tip: use string to measure or trace outline of fruit on paper. Record the exact value. Focus group discussion for third ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked third highest in preference ranking exercise Leaf fresh weight Fresh weight leafFreshMass A leaf weight trait which is the fresh weight of a leaf sword sucker The percentage of plants with any or multiple of the internal disease symptoms - discolouration in the corm, and/or discolouration in the pseudostem, caused by Fusarium wilt. Percentage of plants with internal disease symptoms caused by Fusarium wilt Combined percentage with internal symptoms of Fusarium wilt Fresh weight A plant weight trait which is the fresh weight of a plant Plant fresh weight plantFreshMass Water content - computation (Pseudostem Fresh Weight - Pseudostem Dry Weight) / Pseudostem Fresh Weight Focus group discussion for second lowest ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was second ranked lowest in preference ranking exercise Fresh weight - measurement Weighing sourness scale 0-10 Average lateral diameter avefingerlatdiam The average lateral (side to side) diameter of a finger. Average finger lateral diameter suckerheight Height The height of the pseudostem of the tallest sucker within the same mat as the plant (the sucker that will become the next plant with a bunch, once the bunch from the current plant is harvested) Tallest sucker height Selection criteria for genotype ranked highest by respondents Genotype selection traits Description of traits considered important in selection of best genotype by respondents Calculate as: the sum of the Hand weight measurements from all the hands in the bunch (number equivalent to Number of hands in bunch), e.g. 2.4 + 3.6 + 3.2 + 4.1 + 4.0 + 3.9 + 3.6 + 3.3 + 3.3 + 2.9 + 2.9 + 2.8 = 40.0. Total finger weight - hands - computation Gram accumulated biomass per volume water transpired Transpiration efficiency - computation Population density Number of Pratylenchus spp. per fresh root weight Number of Pratylenchus spp. in roots The date the plant is first recognised as an off-type. dateofftype Date of recognising off-type Date of recognising off-type Taste - estimation Subjective taste assessment of smashed and unsmashed fruit pulp Visually observe the angle of the bunch and choose the option that is most similar, from the 5 schematic drawings of descriptor 6.4.6 in the reference material. Bunch position - estimation mechanical textural attribute relating to the force required to achieve a given deformation, penetration, or breakage of a product. Matooke hardness Matooke MatHardT Hardness in hand bud shape scale (1 to 3) Pigmentation of outer surface Colour of pigmentation on outer surface of cigar leaf on a developed sucker Pigmentation of outer surface of cigar leaf on sucker roots System water loss The amount of water loss in the system over time waterLossSyst Volume Time from start of crop cycle to first external disease symptoms caused by Fusarium wilt Time from start of crop cycle to appearance of external disease symptoms caused by Fusarium wilt The time elapsed from the start of the crop cycle to the appearance on the plant of any of the external disease symptoms - - yellowing leaves, splitting pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Number of nematodes (Hoplolaimus spp.) per unit fresh root weight - method Count from 100 ml of soil sample yellowness scale 0-10 Visual counting method Number of adult larvae - counting Calculate as: the time elapsed between the Date of shooting to the Date of harvest. E.g. 24/12/2014 - 24/09/2014 = 91 days. Time from shooting to harvest - computation Number of leaves with black leaf streak disease severity grade 3 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 3. Calculate as: 100 multiplied by (Youngest leaf spotted, minus 1), divided by Number of standing leaves, e.g. 100 * (6 - 1) / 12 = 42. Index of non-spotted leaves - computation Number of plants not harvested - computation Calculate as: the Number of plants planted, minus the Number of plants dead, minus the Number of plants harvested, e.g. 24 - 4 - 18 = 2. Texture Fruit unsmashed pulp texture The texture of the unsmashed pulp of a mature fruit Press a piece of banana between the molars and appreciate the adhesion of the product Stickiness Sensory Texture method Time from start of crop cycle to first external disease symptoms caused by Fusarium wilt - computation Calculate as: the time elapsed between the Date of planting or the Date of harvest of the bunch from the plant of the previous crop cycle and the Date of first external disease symptoms caused by Fusarium wilt. Weight multfingerweight Multiple finger weight The weight of multiple fingers. Average finger peel thickness - computation Calculate as: the sum of the Finger peel thickness measurements, divided by the number of those measurements, e.g. (2 + 2 + 2 + 3 + 2 + 3 + 3) / 6 = 2. Average actual annual yield actualannyield Average actual annual yield The average actual annual yield, taking into account the proportion of plants harvested, from the number of plants planted Calculate as: the sum of values with a Black leaf streak disease severity grade of 0. Number of leaves with black leaf streak disease severity grade 0 - computation Index of non-spotted leaves Put a part of the sample in the mouth and evaluate the intensity of astringency impression due to the sample Astringency method 5 pt corm discolouration scale Weevil larvae head capsule - measurement Measurement under microscope Number of nematodes (unclassified spp.) per unit fresh root weight - method Count from 100 ml of soil sample Percentage of flower relicts at fruit apex Remains of flower relicts at apex Remains of flower relicts at fruit apex Boiled plantain Boiled plantain Mealiness BoiPlantnMeal Mechanical property linked to cohesion and the presence of fine particles in the product during chewing Mealiness Record the date of event. Date of harvest - estimation Count how many fingers are in a hand. Associate the data with the Hand rank. The recommendation is to collect this data from the second and second-most distal hands. Number of fingers in hand - counting Matooke mechanical textural attribute relating to the force required to remove material that sticks to the mouth Stickiness in hand Matooke stickiness MatStickT Individual female respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. Preference scoring scale female respondents Visual estimation of a fruit fill, (or parthenocarpic) for Early Evaluation Trial stage only. Fruit parthenocarpy - estimation Selection criteria for genotype ranked third lowest by respondents Description of traits considered important in selection of third least genotype by respondents Genotype selection traits pestdiseasesampleoptions Pest/disease assessment/sample collection options The options to record the pest and/or disease assessment and/or sample collection done at the time of data collection. Pest and/or disease assessment and/or sample collection options Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Use colour chart A and observe out of direct sunlight. Record the colour of the margin (general colour is below the rim). [x Observe at flowering time.] Petiole margin colour - estimation BLS An indication of the extent of damage on an individual leaf caused by black leaf streak disease. Black leaf streak disease severity Black leaf streak disease severity Wing presence Behaviour of the petiole margins - winged or not winged. the petiole and pseudostem meet at shooting. Margin is the part of the petiole that can be bent outwards/inwards Petiole margins winged Black leaf streak disease development time - computation Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 and the Date of tagged cigar leaf unrolling at stage B, e.g. 24/06/2014 - 24/03/2014 = 92 days. bract apex scale (1 to 5) Meredith and Lawrence (1969) - complete Extent of root knot nematodes Nematode root knot galling extent The extent of root knot nematodes, observed externally as galls on the roots. Number of banana weevil larvae How many banana weevil larvae caught per trap. Number of banana weevil larvae per trap Fruit pedical length - range - measurement Measure from the scar on the rachis until the beginning of the fruit. Record on the inner fruit in the middle of the mid-hand of the bunch. Tip: use string to measure or trace outline of fruit on paper. Record the range. Record the date of event. Date of first external disease symptoms caused by Fusarium wilt - estimation Number of banana weevil adults Number of banana weevil adults per trap How many banana weevil adults caught per trap. mm pestdiseasesampledescription A description of the pest and/or disease assessment and/or sample collection being done at the time of data collection. Pest and/or disease assessment and/or sample collection description Pest/disease assessment/sample collection description Non-harvest proportion - computation Calculate as: the Number of plants not harvested, divided by the Number of plants planted, e.g. 2 / 24 = 0.08. Colour of the internal face Main colour of the first external unlifted bract internal face Colour of bract internal face Number of Pratylenchus coffeae in roots Population density Number of Pratylenchus coffeae per fresh root weight Youngest leaf spotted The rank (order) of the first fully unfurled leaf with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 leaf spot light-coloued dry centres, caused by black leaf streak disease. Rank of youngest leaf spotted YLS t/unit of area/y Dwarfism of plants dwarfism Dwarfism The presence or absence of dwarfism on a mat Firmness Sensory Texture method Put in the mouth a piece of banana and Evaluate the force necessary to obtain the deformation of the product between the teeth during the first compression The weight of a banana weevil larvae body. Banana weevil larvae body weight Body weight fruit relicts scale (1 to 4) Number of hands on whole bunch Number of hands Number of hands on bunch **subsume Index of black leaf streak disease severity margin behaviour scale (1 to 5) Matooke-like Aroma method Put a part of the product and by retro-olfaction evaluate the presence and the intensity of matoke-like aroma finger The time elapsed from shooting (when the inflorescence emerges from the pseudostem and is still in an erect position) to the harvest of the bunch. shoot2harvest Time from shooting to harvest Time from shooting to harvest Percentage of plants with petiole collapse caused by Fusarium wilt - computation Calculate as: the sum of the number of values for Presence of petiole collapse caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 84 / 300 * 100 = 28%. Moistness Mouth impression method Once the flavors are detected, moisture is felt by turning the product in the mouth dateshoot Date of shooting The date the inflorescence emerges from the pseudostem and is still in an erect position. Date of shooting Genotype selection traits Description of traits considered important in selection of least genotype by respondents Selection criteria for genotype ranked lowest by respondents Number of standing leaves Number of standing leaves The number of leaves attached to the plant that have an erect petiole (not bent or broken). Preference score male respondents Researchers calculate the preference score by male respondents Water loss - measurement Weighing of water loss though system normalized by leaf area The date of death of the plant. Date of death datedeath Date of death Number of plants harvested - computation Calculate as: the sum of the number of values for Date of harvest. Blotches area - estimation Record the relative surface area coverage by blotches. [x Look at several plants if possible to get an overall idea. Observe at flowering time]. The color of the smashed pulp of a mature fruit Fruit smashed pulp colour Colour bad good Try to make a ball (agglomerate) of the sample and evaluate how easy it is to deform or break the sample Moldability in hand method []/cm Index of non-spotted leaves INSL An index to express the proportion of standing leaves without the typical late-stage symptoms of black leaf streak disease, i.e. a black spot with a necrotic centre. This index provides an estimation of available photosynthetic leaf area prior to fruit filling and is a measure of resistance. It also corrects for the difference in the number of leaves produced by different types of bananas. Index of non-spotted leaves leaves/week Rank of oldest standing leaf Rank of oldest standing leaf The rank (order) of the oldest (most-distal) standing leaf (with an erect petiole). The average width of a banana weevil larvae head capsule. Banana weevil larvae average head capsule width Average head capsule width Rank handid The rank (order) of a hand in the bunch. hand rank Hand rank Record the date of event. Date of death - estimation Count how many water suckers are in the mat. Number of water suckers - counting Growth Increase of leaf area Leaf area increase over time leafAreaIncrease Put a part of the sample in your mouth, evaluate during the first bite (between molars) how hard is the sample. Firmness in mouth method The number of sword suckers (suckers with narrow leaves and a large rhizome) in the mat. Number of sword suckers numswordsuckers Number of sword suckers Count how many of the standing leaves are spotted (with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres), considering all leaves in between and inclusive of, the youngest leaf and the oldest standing leaf. Number of spotted leaves - counting Number of fruits on the mid-hand of the bunch **subsume Number of fingers Number of fruits on mid-hand of the bunch Cut a transverse cross section of the corm at 10 cm below the collar (lower cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Damage to lower outer corm weevils - measurement Number Number of dead roots The number of dead roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Boiled plantain Sweetness Elemental flavor caused by dilute aqueous solutions of various substances such as sucrose or aspartame BoiPlantnSwt Sweetness Boiled plantain sucker Count how many sword suckers are in the mat. Number of sword suckers - counting Presence of splitting pseudostem base caused by Fusarium wilt - estimation Visual assessment. datedatacoll Date of data collection Date of data collection The date of data collection. Refers to the first external unlifted bract. Visually observe the apex shape of flattened bracts to determine shape and choose the option that is most similar, from the 4 photos of descriptor 6.5.2. in the reference material. Bract apex shape - estimation Colour of bract external face Colour of the external face Main colour of the first external unlifted bract external face plants not harvested/plants planted Colour line along edge of petiole margin Presence or absence of colour line along edge of petiole margin Presence The pulp aroma of the smashed pulp of a mature fruit Aromas Fruit smashed pulp aroma/smell Number of adult weevils measured How many banana weevil adults used to obtain a measurement. Number of banana weevil adults measured Dry plant growth Growth growthDry Dry mass accumulation over time Overall plant appearance PA Assessment by respondents of physical features of a plant by giving it a score on the preference scale Overall plant appearance The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the colour of the backside middle of tepal. Use colour chart B and observe out of direct sunlight. Compound tepal main colour - estimation Number of standing leaves - counting Count how many of the leaves are standing leaves (without a bent or broken petiole) Chewiness Boiled plantain BoiPlantnChew Boiled plantain Chewiness Energy or number of chews necessary to chew the banana to make it ready to be swallowed Calculate as: number of square metres in the area unit of choice, divided by the Plant spacing, e.g. 10,000 square metres in 1 hectare / 6 = 1,667 plants/ha. Plant density - computation Youngest leaf spotted - counting Record the rank (order) of the youngest leaf spotted (the first fully unfurled leaf with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres), counting the rank by starting with the youngest completely unrolled leaf as 1 and moving downwards, associating the data with the Date of data collection. numnotharv The number of plants that are alive but not harvested (e.g. due to slow or poor development). Number of plants not harvested Combined number of plants not harvested The extent to which vascular discolouration extends up the pseudostem should be determined by making cross-section cuts, from the base of the pseudostem upwards, and examining the internal tissues following each cut. The point at which discolouration is no longer visible should be noted and the distance from this point to the pseudostem base recorded. Extent of internal discolouration in corm of plant caused by Fusarium wilt - estimation Blotch surface area Percentage coverage of petiole base by blotches. Blotches relative area at petiole base Harvest proportion - computation Calculate as: the Number of plants harvested, divided by the Number of plants planted, e.g. 18 / 24 = 0.75. The date of the appearance on the plant of any of the external disease symptoms - yellowing leaves, splitting pseudostem base, changes in new leaves, petiole collapse, caused by Fusarium wilt. Date of appearance of external symptoms caused by Fusairum wilt Date of first external disease symptoms caused by Fusarium wilt Shape Bunch shape Shape of the fully developed bunch on a plant The number of plants that are planted. Combined number of plants planted numplanted Number of plants planted Finger circumference - measurement Measure the circumference of a finger at its widest point, using a tape measure. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. fusion of pedicel scale (1 to 2) plantheightpetioles The height of the pseudostem of the plant, from the collar to the intersection of the petioles of the two youngest leaves. Height Plant height fingercircumf Finger circumference The circumference of a finger. Circumference Cut a transverse cross section of the corm at the collar (upper cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Damage to upper inner corm weevils - measurement Record the date of event. Date of recognising off-type - estimation Fresh weight difference between timepoints (refer to experimental metadata) Growth - computation Percentage of functional roots - computation Calculate as: the Number of functional roots divided by the Number of all roots, multiplied by 100, e.g. 12 / 20 * 100 = 60 % functional roots. Fruit unsmashed pulp taste Taste The taste of the unsmashed pulp of a mature fruit Remains of flower relicts at fruit apex - estimation Visually observe the remains, or not, of flower relicts at the finger apex, and choose the option that is most similar, from the 4 photos of descriptor 6.7.7 in the reference materials. fruit relicts scale (1 to 3) Cut the hands off the rachis and weigh the rachis, using scales. Rachis weight - measurement Calculate as: the Number of dead roots divided by the Number of all roots, multiplied by 100, e.g. 8 / 20 * 100 = 40 % dead roots. Percentage of dead roots - computation Date of tagged cigar leaf unrolling at Bruns stage B - estimation Record the date of event. Finger peel thickness Thickness of the peel peelthickness The thickness of the peel of a finger. (Root Fresh Weight - Root Dry Weight) / Root Fresh Weight Water content - computation Time elapsed from Foures black leaf streak disease severity scale 1 to 6 Black leaf streak disease evolution time The time elapsed between the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1, to 6. DET Time from flowering to harvest The time elapsed from flowering (when the last bracts fall from the most distal hand to display the female flowers) to the harvest of the bunch. Time from flowering to harvest flower2harvest Weight fingerweight Finger weight The weight of a finger. numhands Number of hands in bunch The number of hands in the bunch. Number of hands The relative water content in a pseudostem Content Pseudostem relative water content rwcPseudostem Main colour of the face of anther opposite to dehiscence Anther colour Colour Record the rank (order) of the second-most distal hand in the bunch, starting with the hand at the proximal end (closest to the pseudostem) of the bunch as 1 and continuing to the hand at the most distal end (closest to the male bud). Hand rank - counting yyyy/mm/dd Potential annual yield - computation Calculate as: the Bunch weight divided by 1,000, multiplied by the Annual crop cycle proportion, multiplied by the Plant density, e.g. (50 / 1,000) * 1.09 * 1,667 = 90.85. plants/unit of area Damage percent X-lower-inner Percentage damage to lower cross-section inner corm caused by weevils The amount of damage to the inner corm (central cylinder) lower cross-section, assessed by percentage of tunnels caused by weevils. The weight of a banana weevil adult weevil body. Body weight Banana weevil adult body weight Weigh a finger, using scales. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger weight - measurement fruit curve shape (scale 1 to 6) Moistness scale 0-10 Surface color homogeneity Appearance method When you receive the sample, observe the surface and evaluate the homogeneity Boiled plantain Firmness Force required to obtain deformation, penetration or rupture of the banana Boiled plantain Firmness BoiPlantnFirm finger The length of a pseudostem Length lengthPseudostem Pseudostem length Hand rank of 2nd-most distal hand The rank (order) of a the second-most distal hand in the bunch. Rank handid2ndmostdistal Calculate as: the time elapsed between the Date of planting to the Date of harvest. E.g. 24/12/2014 - 24/01/2014 = 334 days. Plant crop cycle - computation Apex shape Shape of the first external unlifted bract of the male bud Bract apex shape Combined percentage with yellowing leaves Percentage of plants with yellowing leaves, caused by Fusarium wilt. Percentage of plants with yellowing leaves caused by Fusarium wilt Functional leaf number The number of functional leaves (greenness>50%) in a shoot system Number numFuncLeaves Sourness Matooke MatSour gustatory complex sensation, generally due to presence of organic acids Matooke sourness Ratio Ratio root to shoot rootShootDry The ratio of dry mass of belowground (root) to abovegrund (shoot) biomass Number of Rotylenchulus spp.in soil Number of Rotylenchulus spp.per soil sample Population density Focus group discussion for second lowest ranked traits with male respondents Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked second lowest in preference ranking exercise cm? The number of leaves with at least 10 discrete, mature, necrotic lesions or one large necrotic area with 10 light-coloured dry centres, caused by black leaf streak disease. Number of spotted leaves Number of spotted leaves The date a youngest leaf spotted is first observed on the plant, caused by black leaf streak disease. Date of first youngest leaf spotted Date of first youngest leaf spotted Average percentage damage to the corm caused by weevils Damage percent X-average The average percentage of damage in the whole corm (the inner corm and the outer corm) caused by weevils. Time from harvest to death - computation Calculate as: the time elapsed between the Date of harvest of the bunch of the plant from the preceeding crop cycle and the Date of death of the current plant, e.g. 24/03/2015 - 24/12/2014 = 90 days. Vitamin C - measurement Calculate as: the sum of the number of values for Presence of petiole collapse caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 84 / 300 * 100 = 28%. Chewiness Harvest proportion Combined proportion of plants harvested harvprop The proportion of plants that are harvested, from the number of plants planted. wing presence/absence and clasping presence/absence scale 1 to 5 Presence of yellowing leaves caused by Fusarium wilt - estimation Visual assessment. Calculate as: the sum of the number of values for Presence of yellowing leaves caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 78 / 300 * 100 = 26%. Percentage of plants with yellowing leaves caused by Fusarium wilt - computation The presence of root knot nematodes, observed externally as galls on the roots. Presence of root knot nematodes Nematode root knot galling presence End date and time of data collection - estimation Record the date and time of event. Extent of internal discolouration in corm of tallest sucker caused by Fusarium wilt Extent of discolouration The extent of discolouration in the corm of the tallest sucker, caused by Fusarium wilt. The overall estimation of fruit parthenocarpy level on a bunch Parthenocarpy Parthenocarpy goodfruitfill Rank of oldest standing leaf - counting Record the rank (order) of the oldest standing leaf (with an erect petiole), counting the rank by starting with the youngest completely unrolled leaf as 1 and moving downwards, associating the data with the Date of data collection. Number of leaves with black leaf streak disease severity grade 2 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 2. singleLeafArea Area Leaf area The area of a leaf Time from planting to shooting plant2shoot Time from planting to shooting The time elapsed from planting to shooting (when the inflorescence emerges from the pseudostem and is still in an erect position). Weighing of water loss though system between timepoints (refer to experimental metadata) Water loss - measurement A description of the damage to the plant. Description of damage Damage description describedamage Rank of previously marked leaf rankprevleaf Rank The current rank (position) of the leaf that was previously marked as the youngest leaf at the time of the last data collection event. Number used to obtain a measurement The number of fingers used to obtain a measurement. numfingersmeas Number of fingers measured Count from 10 g of chopped roots from the tested banana plant sample Number of nematodes (Pratylenchus spp.) per unit fresh root weight -method nematode lifecycle stages The average length of a banana weevil adult body, from the head to the tip of the abdomen. Average body length Banana weevil adult average body length Nematode multiplication from single root inoculation of 50 nematodes per 8 cm length after 12 weeks Population density Nematode reproduction factor (microplot screening) Force required to peel off the fraction of product adhering to the interior of the oral cavity BoiPlantnStic Stickiness Boiled plantain Stickiness Boiled plantain Calculate as: the Bunch weight multiplied by the Annual crop cycle proportion, e.g. 48 * 1.09 = 52.32. Annual plant productivity - computation The Vitamin C content of freeze-dried smashed pulp of a mature fruit Vitamin C content Vitamin C content height score (1 to 3) Cause of death - estimation Record the apparent cause of death of the plant, e.g. strong winds, Fusarium wilt, etc. bract behaviour scale (1 to 2) The average weight of a banana weevil adult body. Banana weevil adult average body weight Average body weight Banana pest disease Ratio - computation Ratio of belowground (root) to aboveground (shoot) dry mass Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 - estimation Record the date of event. LER A rate that expresses the number of leaves that have emerged from the pseudostem in a month. Monthly leaf emission rate Monthly leaf emission rate The number of plants that are dead, in a defined area. deathdens Combined density of plants that are dead Death density Manual height measurement Length - measurement Average head capsule width Banana weevil adult average head capsule width The average width of a banana weevil adult head. The date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1. Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 Date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1 Sweetness tasting method Put a part of the sample in the mouth and evaluate the intensity of taste of sweetness The length of a leaf Length singleLeafLength Single leaf length Visually observe the part of the rachis between the last hand and the male bud. Truncated means there is no bract scar below the last hand. Present means there is a degenerated or persistent male bud Male rachis type - estimation Growth Fresh plant growth growthFresh Fresh mass accumulation over time Time elapsed from Bruns stage B of unrolling to Foures black leaf streak disease severity scale 6 DDT The time elapsed between a tagged cigar leaf at Bruns stage B of unrolling and the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6. Black leaf streak disease development time Time from flowering to harvest - computation Calculate as: the time elapsed between the Date of flowering to the Date of harvest, e.g. 24/12/2014 - 24/10/2014 = 61 days. Number of nematodes (Meloidogyne spp.) per unit fresh root weight -method Count from 10 g of chopped roots from the tested banana plant sample Calculation of preference score by respondents Overall plant appearance Preference score PS The time elapsed from planting to the death of the plant. Time from planting to death Time from planting to death plant2death Visual observation Peripheral damage - measurement Calculate as: the Plant density multiplied by the Harvest proportion, e.g. 1,667 * 0.75 = 1,250. Harvest density - computation blotch pigmentation scale (1 to 5) Number of leaves with black leaf streak disease severity grade 6 - computation Calculate as: the sum of values with a Black leaf streak disease severity grade of 6. Visual assessment. Presence of petiole collapse caused by Fusarium wilt - estimation Matooke astringency complex sensation, accompanied by shrinking, drawing or puckering of the skin or mucosal surface in the mouth, produced by substances such as kaki tannins or sloe tannins Matooke Astringency MatAstr Finger feel assessment of smashed and unsmashed fruit pulp Finger feel - estimation rachis appearance scale (1 to 5) Dry weight - measurement 80?C, for 14 days, or longer untill dry Black leaf streak disease severity - estimation Visually observe and record the amount of the surface area of a standing leaf that is affected by black leaf streak disease. leaf Nematode stage - estimation Identify the nematode stage, using the options. Count how many fingers are in the second-most distal hand. Number of fingers in 2nd-most distal hand - counting Presence absence 1 Preference scale Open question Mealiness Sensory Texture method Put a piece of banana in your mouth and assess the presence of mealiness particles during chewing 80?C, for 14 days, or longer untill dry Dry weight - measurement Actual annual yield - computation Calculate as: the Bunch weight divided by 1,000, multiplied by the Annual crop cycle proportion, multiplied by the Harvest density, e.g. (50 / 1,000) * 1.09 * 1,250 = 68.13. Damage to upper outer corm weevils - measurement Cut a transverse cross section of the corm at the collar (upper cross-section). Score weevil damage (galleries) as percentage damage on the side of the cross-section that was facing the ground, downwards. Calculate as: the sum of values with a Black leaf streak disease severity grade of 1. Number of leaves with black leaf streak disease severity grade 1 - computation Visual assessment. Presence of splitting pseudostem base caused by Fusarium wilt - estimation Length Length of male bud from end of rachis to apex of the bud Male bud length Calculate as: multiply the distance of the spacing between the plants, both latitudinally and longitudinally, e.g. 2 m x 3 m = 6 m2. Plant spacing - computation Dry weight difference between timepoints (refer to experimental metadata) Growth - computation Combined weight totalfingerweight The weight of all the hands of the bunch, excluding the rachis. Total finger weight Chlorosis Leaf chlorosis Leaf trait correlated to the health stage of the leaf leafChlorosis Count how many peeper suckers are in the mat. Number of peeper suckers - counting Number of functional roots - counting Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. Count the number of functional roots. Alignment of bracts at the apex of the male bud Bract imbrication at apex of male bud Imbrication The taste of the smashed pulp of a mature fruit Taste Fruit smashed pulp taste Visually observe the male rachis position (the part between the last hand and the male bud) and choose the option that is most similar, from the 5 schematic drawings of descriptor 6.4.12 in the reference material. Male rachis position - estimation Banana weevil larvae head capsule width The width of a weevil larvae head capsule. Head capsule width Number of fingers in 2nd hand - counting Count how many fingers are in the third hand. Weight bunchweight The weight of the bunch, including the rachis. Bunch weight Distance from the base of the pseudostem to the emerging point of the peduncle. Height Pseudostem height Weight The weight of a hand. Hand weight handweight Meredith and Lawrence (1969) - condensed Visual assessment. Presence of changes in new leaves caused by Fusarium wilt - estimation Taste method Sweetness Put a piece of banana in your mouth, chew it and swirl it around your tongue to detect the sweet flavor maiden sucker Number of adult weevils - counting Visual counting method of Weevils per trap (the trap should be minimum 30 cm long) Calculate as: the sum of values with a Black leaf streak disease severity grade of 5. Number of leaves with black leaf streak disease severity grade 5 - computation Egg-laying female root knot nematode Count how many maiden suckers are in the mat. Number of maiden suckers - counting (Plant Fresh Weight - Plant Dry Weight) / Plant Fresh Weight Water content - computation open answer Count from 100 ml of soil sample Number of nematodes (Meloidogyne spp.) per unit fresh root weight - method The extent of egg-laying female root knot nematodes, observed internally as pit-like structures on the roots. Extent of root knot nematodes Nematode egg-laying females extent rachis type scale (1 to 2) Presence Behaviour of male rachis - truncated or present. Truncated means there is no bract scar below the last hand. Present means there is a degenerated or persistent male bud Male rachis type Fruit length - exact value - measurement Measure the length of the internal arc of a fruit, without pedicel. Record on the inner fruit in the middle of the mid-hand of the bunch. If there is an even number of hands, there will be two middle hands so use the upper hand that developed first. Record the exact value. Percentage of plants with a splitting pseudostem base caused by Fusarium wilt - computation Calculate as: the sum of the number of values for Presence of splitting pseudostem base caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 24 / 300 * 100 = 8%. Root nematode density The number of nematode individuals per unit of fresh root weight. Number of nematodes per unit of fresh weight Matooke-like aroma Matooke Aroma of the local matooke. MatAroMatA Matooke aroma matooke Tallest sucker height - measurement On the tallest sucker, measure the distance from the pseudostem base at the ground to the intersection of the petioles of the two youngest leaves (leaf ranks 1 and 2), using a measuring pole or sliding ruler. Average finger circumference avefingercircumf Average circumference The average circumference of the fingers. Average external length avefingerextlength Average finger external length The average length of the fingers measured along the external (dorsal) arc, excluding the pedicel and the fruit tip. Visual counting method of Weevil traps (the trap should be minimum 30 cm long). Number of adult weevil trap - counting Average finger weight - computation Calculate as: the sum of the the Finger weight measurements, divided by the number of those measurements, e.g. (150 + 146 + 147 + 152 + 140 + 146) / 6 = 147. Measure the distance from the collar, or from the pseudostem base at the ground if the collar is not visible, to under the neck of the curved peduncle, using a measuring pole or sliding ruler. Plant height - neck of peduncle - measurement Number of suckers - counting Count how many of all types of suckers are in the mat. Record the rank of the previously marked leaf (the leaf that was marked in the Rank of marked youngest leaf during the last data collection), counting the leaf rank by starting with the newest completely unrolled leaf as 1 and moving downwards. Associate the data with the Date of data collection. Rank of previously marked leaf - counting g / mL Number of fingers measured - counting Count how many fingers are used to obtain a measurement. Focus group discussion with male respondents, to discuss selection traits of genotype that was ranked second highest in preference ranking exercise Focus group discussion for second ranked traits with male respondents Average finger peel thickness The average thickness of the peel of a finger. Average thickness of the peel avepeelthickness Mealiness scale 0-10 goodbunch Quality Overall bunch quality The overall estimation of the bunch quality on a mat YL The presence/absence of yellowing leaves, caused by Fusarium wilt. Presence/absence of yellowing Presence of yellowing leaves caused by Fusarium wilt mm kg Record the date of event. Date of shooting - estimation Cause of death Cause of death causedeath The cause of death of the plant. The average number of fingers in a hand. Average number of fingers in hand Average number of fingers avenumfingshand Color of the surface of the sample from light yellow to bright yellow Matooke Matooke surface yellowness intensity Surface yellowness intensity MatSurfYellV Date of damage - estimation Record the date of event. bunch shape scale (1 to 5) Count from 100 ml of soil sample Number of nematodes (Pratylenchus goodeyi) per unit fresh root weight - method Dry weight - measurement 80?C, for 14 days, or longer untill dry Measure the length of a finger, along the internal (ventral) arc, excluding the pedicel and the fruit tip, using a tape measure. Associate the data with the Hand rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Finger internal length - measurement Lobe colour of tip of compound tepal - estimation The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the colour of the lobe at the tip of the tepal. Use colour chart B and observe out of direct sunlight. Cut multiple hands from the rachis and weigh them together, using scales. Associate the data with the Hank rank and the Number of hands measured. Multiple hand weight - measurement Calculate as: the sum of the number of plants with any or multiple of the internal disease symptoms caused by Fusarium wilt - discolouration in the corm, and/or discolouration in the pseudostem - divided by the Number of plants planted * 100, e.g. 108 / 300 * 100 = 36%. Percentage of plants with internal disease symptoms caused by Fusarium wilt - computation Description of how off-type A description of what made the plant recogniseable as an off-type. describeofftype Off-type description Body length The length of a banana weevil adult body, from the head to the tip of the abdomen. Banana weevil adult body length Plant state description A description of the state of the plant. State description statedescription Bract behaviour before falling - estimation Visually observe the last bract to lift from the hands, ideally when the bract has lifted to a horizontal position, and choose the option that is most similar, from the 2 photos of descriptpr 6.5.12 in the reference material. Percentage of plants with changes in new leaves caused by Fusarium wilt - computation Calculate as: the sum of the number of values for Presence of changes in new leaves caused by Fusarium wilt scored as Present, divided by the Number of plants planted, multiplied by 100, e.g. 67 / 300 * 100 = 22%. Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant, do not include roots from adjacent plants. Carefully wash the soil from the roots with tap water. Cut the roots into 10 cm long pieces and dry with paper tissue. Take a subsample of 15 g, add 100 ml distilled water and store the roots at 4 degrees Celsius. Put the roots in 100 ml of distilled water in a kitchen blender. Macerate the roots 3 times for 10 secs (separated by 5 sec intervals). Pour the macerated suspension through 250, 106 and 40 micro-metre sieves and rinse the sieves with tap water. Using distilled water, collect in a beaker the nematodes from the 40 micro-metre sieve. Using distilled water, dilute to 200 ml the nematode suspension in a graduated cylinder. Blow air through the nematode suspension with a pipette (to homogenise the suspension). Take a subsample of 6 ml (counting dish) or 2 ml (counting slide). Count the nematodes in the counting dish (stereo microscope) or in the counting slide (light microscope). Calculate the final nematode population per unit of fresh root weight. Root nematode density - computation Weigh multiple fingers together, using scales. Associate the data with the Hank rank and the Number of fingers measured. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Multiple finger weight - measurement The number of leaves on the plant that have 50 % or more of the leaf surface area as green, healthy, photosynthetic tissue. Number of functional leaves functleaves Number of functional leaves Count from 100 ml of soil sample Number of nematodes (Pratylenchus spp.) per unit fresh root weight - method Surface yellowness intensity Appearance method When you receive the sample, observe the surface and evaluate the intensity of the color Visually observe the fusion of pedicels and choose the option that is most similar, from the 3 photos of descriptor 6.7.11. in the reference materials. Observe before they join the rachis at the bract scar. Look upwards from the bottom of the bunch. Fusion of pedicels - estimation firmness scale 0-10 kg/plant/y peeper sucker Collect all roots from a standard size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Carefully wash the soil from the roots with tap water. Cut the roots into 1 cm pieces. Take a subsample of 5 g, add 100 ml distilled water and store the roots at 4 degrees Celsius. Stain the egg masses by immersing the roots in 0.15 g/L phloxine B for 15 mins. Count the number of egg-laying females under a stereo microscope. Nematode egg-laying females extent - estimation plantcircumf20cm The circumference of the pseudostem of the plant at 20 cm from the collar. Plant circumference 20 cm from the collar Circumference 20 cm above the ground clasping or not clasping sweetness scale 0-10 Calculate as: the sum of the Fruit pulp lateral diameter measurements, divided by the number of those measurements, e.g. (3.1 + 4.8 + 3.7 + 4.6 + 5.6 + 6.4) / 6 = 4.7. Average fruit pulp lateral diameter - computation (Leaf Fresh Weight - Leaf Dry Weight) / Leaf Fresh Weight Water content - computation Male bud shoulder description Male bud shoulder Ratio Calculate as: the sum of the Number of fingers in hand measurements from all the hands in the bunch (number equivalent to Number of hands in bunch), e.g. 16 + 17 + 19 + 18 + 19 + 18 + 17 + 17 + 16 + 14 + 14 + 13 = 198. Number of fingers in bunch - computation The number of water suckers (suckers with broad leaves, a small rhizome, and a weak connection to the plant) in the mat. numwatersuckers Number of water suckers Number of water suckers Average damage to the corm weevils - computation Calculate as: the sum of Damage to inner corm upper cross-section caused by weevils, Damage to outer corm upper cross-section caused by weevils, Damage to inner corm lower cross-section caused by weevils, and Damage to outer corm lower cross-section caused by weevils, divided by the number of traits included in the sum, e.g. (25 + 36 + 18 + 20) / 4 = 24.8. Length and maximum diamter of male bud at harvest. Male bud size - measurement bud shoulder scale (1 to 3) Percentage of plants with a splitting pseudostem base, caused by Fusarium wilt. Percentage of plants with a splitting pseudostem base caused by Fusarium wilt Combined percentage with splitting pseudostem base Circumference 75 cm above the ground Plant circumference 75 cm from the collar The circumference of the pseudostem of the plant at 75 cm from the collar. plantcircumf75cm Number of Radopholus similis in roots Population density Number of Radopholus similis per fresh root weight plantcircumfcollar Plant circumference at collar The circumference of the pseudostem of the plant at the collar. Circumference at collar Main colour Main colour of the backside of the compound tepal Compound tepal main colour Calculate the ratio w/y, i.e. the broadest width of the male bud to the total length of the male bud. Do not measure the dimensions along the bud but rather on a projection/outline of the bud (e.g. trace the outline of the bud on paper) and use ruler or measuring tape Male bud shoulder - computation Weighing Fresh weight - measurement MatAroGrassA Matooke aroma grassy Matooke Grassy-like aroma Aroma of fresh grass Researchers calculate the preference score by female respondents Preference score female respondents dd/mm/yyyy hh:mm:ss Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 and the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1, e.g. 24/06/2014 - 24/04/2014 = 61 days. Black leaf streak disease evolution time - computation Estimate maturity stage of the bunch. Bunch maturity stage - estimation Non-harvest density - computation Calculate as: the Plant density multiplied by the Non-harvest proportion, e.g. 1,667 * 0.08 = 133. The rank (position) of the youngest completely unrolled leaf. Rank rankyoungleaf Rank of marked youngest leaf harvest2death The time elapsed from the harvest of the bunch of the plant from the preceding crop cycle to the death of the plant from the current crop cycle. Time from harvest to death Time from harvest to death aroma scale 0-10 Root knot nematode galling Leaf area difference between timepoints (refer to experimental metadata) Growth - computation Lateral diameter of the pulp The lateral (side to side) diameter of the pulp of a fruit. Fruit pulp lateral diameter pulpdiam bud size scale (1 to 3) BD colour chart A - 2016 Describe the pest and/or disease assessment and/or sample collection taking place at the time of data collection. Pest and/or disease assessment and/or sample collection description - estimation Leaf area - measurement Top view image Number of fingers in 3rd hand - counting Count how many fingers are in the third hand. open answer Measure for the biomass allocation: dry mass of roots versus whole plant dry mass ratio Ratio rootToPlantMassRatio_dry Root to whole plant mass ratio Ratio of leaf dry mass versus plant dry mass Ratio - computation water suckers rachis angle scale (1 to 2) Plant height to neck of peduncle Height The height of the pseudostem of the plant from the collar to the curved neck of the peduncle. plantheightneck hardness scale 0-10 blotch color scale (1 to 4) hand Plant state description - estimation Record a description of the state of the plant. Focus group discussion for top traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked highest in preference ranking exercise margin behaviour scale (1 to 4) Measure the length of the internal arc of a fruit, without pedicel. Record on the inner fruit in the middle of the mid-hand of the bunch. If there is an even number of hands, there will be two middle hands so use the upper hand that developed first. Record the range. Fruit length - range - measurement Number of leaves with a black leaf streak disease severity grade of 1 The number of leaves on a plant with a black leaf streak disease severity grade of 1. Number of leaves with black leaf streak disease severity grade 1 Visually observe the shape of the mature bunch on a fully developed plant that is not experiencing environmental stress and choose the option that is most similar, from the 4 photos of descriptor 6.4.7. in the reference material. Bunch shape - estimation Identify the nematode species present in the banana plant root sample (Radopholus similis, Pratylenchus coffeae, Pratylenchus goodeyi, Helicotylenchus multicinctus, Meloidogyne incognita, ?) Nematode species - estimation Number of fingers The number of fingers in multiple hands. Multiple number of fingers in hand multnumfingshand 2 pt scale The overall estimation of sucker quality on a mat Overall suckering quality Sucker quality suckering Number of suckers The number of all types of suckers in the mat. numsuckers Number of all types of suckers Efficiency The growth (weight increase) per volume transpired water Transpiration efficiency transpirationEfficiency Damage to periphery of peeled corm caused by weevils The percentage of damage to the periphery of the peeled corm, assessed by tunnels caused by weevils Peripheral damage caused by weevils Calculate as: the time elapsed between the Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 1 and the Date of tagged cigar leaf unrolling at stage B, e.g. 24/04/2014 - 24/03/2014 = 31 days. Black leaf streak disease incubation time - computation Top view image of a single leave Leaf area - measurement Time elapsed from planting to first youngest leaf spotted Time from planting to first youngest leaf spotted Time elapsed between planting and the appearance of the first youngest leaf spotted. Preference scoring scale individual respondents Individual respondents give the plant one of three possible scoring cards: Like, Dont know, Dont like. Men and women use different colors of scoring cards. dwarfism BD colour chart A - 1996 parthenocarpy Percentage of dead roots Percentage of dead roots The percentage of all roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant that are dead. Calculate as: the sum of values with a Black leaf streak disease severity grade of 4. Number of leaves with black leaf streak disease severity grade 4 - computation Calculate as: the sum of the number of values for Date of death. Number of plants dead - computation estnumfingers Number of fingers The estimated number of fingers in the bunch based on a calculation using the number of fingers in the third and second-most distal hands and the number of hands in the bunch. Calculated number of fingers in bunch Anther colour - estimation The following flower descriptors refer to the flowers at the axil of the first external unlifted bract. Fresh material must be used (make the observation as soon as you detach the bract/flower from the rachis). Visually observe the anther colour on the face opposite to the dehiscence split of the anther. Use colour chart B and observe out of direct sunlight. pseudostemHeightIncrease Pseudostem height increase over time Height increase of pseudostem Growth leaf rank Population density Number of Hoplolaimus spp. per soil sample Number of Hoplolaimus spp. in soil Date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6 Date of tagged leaf presenting black leaf streak disease symptoms at Foures stage 6 The date a tagged leaf presents black leaf streak disease symptoms at Foures black leaf streak disease severity grade 6. The colour of the fruit pulp. Fruit pulp colour fruitpulpcolour Pulp colour Fruit pulp lateral diameter - measurement Cut a finger in half, crosswise at its widest point, and on the cross-section measure the lateral diameter of the fruit pulp, from the left to the right side (not from the ventral to the dorsal side), using calipers. Associate the data with the Hand rank. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Angle of the rachis between last hand and male bud Angle of the part of the rachis between the last hand and the male bud Male rachis position Average weight avefingerweight Average finger weight The average weight of the fingers. Visually observe the fruit pulp colour and select the option in the colour chart that is most representative. The recommendation is to collect this data from six fingers in total - three fingers in the middle of the outer whorl from the second hand and from the second-most distal hand. Fruit pulp colour - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Use colour chart A and observe out of direct sunlight. Record the colour of the margin (general colour is below the rim). [x Observe at flowering time.] Petiole margin colour - estimation Number of fingers The number of fingers in the third hand. numfingers3rdhand Number of fingers in 3rd hand fruit pedicel scale (1 to 3) The total number of all roots (both dead and functional) in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Number of all roots Number bud length scale (1 to 3) Main underlying colour Main underlying colour of pseudostem Main colour of the pseudostem under the outermost sheath. Weevil larvae body weight - measurement Measurement under microscope The number of functional roots in a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Number Number of functional roots Calculate as: the sum of the number of values for Date of planting. Number of plants planted - computation bunch maturity Collect all roots from a standard-size excavation of 20 x 20 x 20 cm extending outwards from the corm of the plant. Take only roots from the selected plant. Divide the collected roots into two categories: dead roots, functional roots. Dead roots are completely rotten or shrivelled whereas functional roots show at least some healthy tissue. From the functional roots, randomly select five primary roots that are at least 10 cm, and reduce their length to 10 cm. Determine the presence/absence of root knot nematodes, observed externally as galls on the roots. Nematode root knot galling presence - estimation Cut a hand from the rachis and weigh the hand, using scales. Associate the data with the Hand rank. Hand weight - measurement Date of weevil damage to corm assessment The date the assessment of weevil damage to the corm of the plant is conducted. Date of weevil damage assessment Ratoon crop cycle - computation Calculate as: the time elapsed between the Date of harvest of the bunch of the plant from the preceeding crop cycle and the Date of harvest of the bunch of the plant from the current crop cycle, e.g. 24/11/2015 - 24/12/2014 = 335 days. Petiole margin clasping - estimation Observations on the margins and petiole wings should be made where the petiole and pseudostem meet at shooting. Observation should be made at shooting on the neck, where the petiole and pseudostem meet. Margin is the part of the petiole that can be bent outwards/inwards. [x Observe at flowering time.] nonharvdens Non-harvest density Combined density of plants not harvested The number of plants that are alive but not harvested (e.g. due to slow or poor development), in a defined area. Black leaf streak disease incubation time Time elapsed from Bruns stage B of unrolling to Foures black leaf streak disease severity scale 1 The time elapsed between a tagged cigar leaf at Bruns stage B of unrolling and the development of black leaf streak disease symptoms at Foures black leaf streak disease severity grade 1. DIT Fruit pendulance - estimation Visual estimation of fruit pendulance on a bunch, for Early Evaluation Trial stage only. Sweetness scale 0-10 BD colour chart A - 2016 Fusarium wilt external symptoms scale Focus group discussion for lowest ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked lowest in preference ranking exercise Fusarium wilt internal symptoms scale Leaf length - measurement The longest diagonal of a fitted ellips Banana weevil adult head capsule width Head capsule width The width of a banana weevil adult weevil head. multiplication factor The nematode species found in the banana plant root sample. Species present Nematode species The number of plants that are harvested. Number of plants harvested Combined number of plants harvested numharv Presence absence 0 hands variable_of bunch position scale(1 to 5) fruit apex scale (1 to 5) crop cycle duration/year method_of Focus group discussion for second ranked traits with female respondents Focus group discussion with female respondents, to discuss selection traits of genotype that was ranked second highest in preference ranking exercise Nematode lifecycle stage Lifecycle stages present The lifecycle stage(s) of the nematode species found in the banana plant root sample. Tallest sucker number of functional leaves - counting Count how many functional leaves (leaves that have 50% or more of their surface as green, healthy, photosynthetic tissue) are on the tallest sucker. Plant spacing plantspacing The area allocated to one plant. Area allocated to one plant The extent to which vascular discolouration extends up the pseudostem of the tallest sucker should be determined by making cross-section cuts, from the base of the pseudostem upwards, and examining the internal tissues following each cut. The point at which discolouration is no longer visible should be noted and the distance from this point to the pseudostem base recorded. Extent of internal discolouration in corm of tallest sucker caused by Fusarium wilt - estimation Percentage damage to upper cross-section outer corm caused by weevils Damage percent X-upper-outer The amount of damage to the outer corm (cortex) upper cross-section, assessed by percentage of tunnels caused by weevils. No. per 100ml petiole-margin contrast color homogeneity scale 0-10 Faures stages of development of BLS disease scale (1987)